TTC28 cDNA ORF clone, Bos taurus(cattle)

The following TTC28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224678 XM_024977805.1
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa224679 XM_024977806.1
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa224680 XM_024977807.1
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa224681 XM_024977808.1
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa93256 XM_015475572.1
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa93256 XM_617836.9
Latest version!
Bos taurus tetratricopeptide repeat domain 28 (TTC28), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224678
    Clone ID Related Accession (Same CDS sequence) XM_024977805.1
    Accession Version XM_024977805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCAGT CACCGCCGCC CGCGCCTGAG CCCACCCCGG GGTCGACCCC AGCACGGAGT 
    CGTAGACGAC GGGAGGCCGA GTCGCCGCCG GCGCCGGCTC CGATTCCTCT CTTTGGTGCT
    AAGACTATTG GCCGGAGAAG TCCTGATGGA CCAGTGCTGA GCAAAGCTGA ATTTGTTGAG
    AAAGTTCGTC AGAGTAATCA GGCCTGTCAT GATGGAGATT TCCACACAGC TATTGTTCTC
    TACAATGAAG CCCTGGCTGT TGACCCTCAG AACTGTATCT TATACAGCAA TAGATCTGCA
    GCCTATATGA AAATCCAGCA GTATGACAAG GCACTGGATG ATGCAATCAA AGCTCGACTT
    CTCAATCCCA AGTGGCCAAA GGCATACTTC CGACAGGGTG TTGCCCTCCA GTACCTCGGA
    CGTCATGCCG ATGCCCTGGC AGCCTTTGCG TCTGGGTTGG CTCAGGACCC CAAGAGTCTC
    CAGCTTCTGG TGGGGATGGT AGAAGCAGCC ATGAAATCTC CCATGAGAGA CTCCCTCGAA
    CCCACTTATC AGCAGCTTCA GAAAATGAAA CTGGACAAGA GCCCCTTTGT GGTTGTCTCT
    GTGGTTGGAC AGGAGCTCCT GACAGCTGGC CATCACGGGG CCTCTGTGGT TGTCTTAGAA
    GCCGCTCTGA AGATTGGCAC CTGCAGCCTC AAGCTGAGAG GTTCCGTTTT CTCTGCACTG
    AGCAGTGCTT ACTGGTCTCT CGGGAACACA GAGAAGAGCA CTGGATACAT GCAGCAGGAC
    TTGGATGTAG CCAAGACCTT AGGTGACCAG ACAGGAGAAT GCCGAGCTCA TGGGAACCTG
    GGCTCTGCAT TCTTCTCCAA AGGAAATTAC CGGGAGGCTC TCACTAACCA CAGGCATCAG
    TTGGTGCTTG CCATGAAACT CAAGGATCGA GAGGCAGCTT CATCAGCCCT GAGCAGTCTG
    GGCCACGTGT ACACAGCCAT TGGAGATTAC CCCAATGCAC TGGCCAGTCA CAAACAGTGT
    GTTCTTCTCG CCAAGCAATC CAAAGATGAG CTTTCTGAAG CCCGAGAACT CGGCAACATG
    GGAGCTGTGT ATATTGCCAT GGGTGACTTT GAGAATGCTG TGCAGTGCCA CGAGCAGCAT
    CTGAAGATAG CCAAGGACCT GGGGAACAAG CGAGAAGAAG CCCGGGCCTA CAGCAATCTG
    GGCAGTGCCT ATCACTACAG GAGGAACTTT GACAAGGCCA TGTCTTACCA CAATTACGTG
    CTGGAGCTGG CGCAGGAGCT GATGGAGAAG GCCATTGAGA TGCGGGCCTA CGCTGGCCTG
    GGCCACGCTG CCAGGTGCAT GCAGGATTTG GAGAGGGCCA AACAGTACCA CGAGCAGCAG
    CTGGGCATTG CTGAGGATCT CAAGGACCGG GCTGCAGAGG GGCGAGCGTC TTCCAATCTG
    GGAATCATAC ACCAGATGAA AGGTGATTAT GACACTGCAC TGAAACTCCA CAAGACCCAC
    TTGTGCATCG CCCAGGAGCT GAGTGACTAC GCGGCCCAGG GCCGCGCCTA TGGAAACATG
    GGCAACGCCT ACAATGCTCT GGGCATGTAC GACCAAGCGG TCAAGTACCA CCGGCAGGAG
    CTACAGATCT CCATGGAAGT GAACGACCGT GCCTCACAGG CATCCACGCA CGGGAACCTC
    GCTGTGGCCT ACCAGGCGCT GGGCGCCCAC GACCGGGCCC TGCAGCACTA TCAGAACCAT
    TTGAACATCG CCCGGGAGCT GCGAGACATC CAGAGCGAGG CGCGGGCTCT CAGCAACCTG
    GGCAACTTCC ACTGCTCTCG AGGGGAGTAC GTCCAGGCCG CCCCCTATTA CGAACAGTAC
    CTCCGGCTTG CTCCTGACCT TCAAGACATG GAGGGAGAAG GGAAGGTCTG CCACAACCTC
    GGCTATGCCC ATTACTGCCT TGGAAATTAC CAGGAGGCGG TAAAGTACTA TGAGCAGGAT
    CTGGCCCTGG CCAAAGACCT TCACGACAAA TTGAGTCAAG CAAAAGCTTA CTGCAACTTG
    GGCTTGGCAT TCAAGGCTCT GCTGAATTTC AGCAAAGCTG AAGAGTGTCA AAAGTACCTA
    CTGTCCCTAG CCCAGTCCCT GAATAATTCC CAGGCTAAAT TTAGAGCCCT GGGGAACCTG
    GGCGATATAT TCATCTGTAA AAAAGATATC AATGGTGCAA TAAAATTCTA TGAGCAGCAG
    CTGGGCTTGG CTCACCACGT GAAGGACAGG AGACTGGAGG CCAGTGCATA TGCAGCCCTG
    GGCACTGCAT ACCGAATGAT CCAGAAACAC GACAAGGCCT TGGGTTATCA CACACAGGAG
    CTGGAAGTAT ATCAGGAGCT GAGTGACCTG CCGGGAGAGT GCAGAGCTCA TGGGCACCTG
    GCCGCAGTCT ACATGGCCCT GGGGAAGTAC ACAATGGCTT TCAAGTGTTA CGAAGAGCAG
    CTGGATCTAG GGCAGAAGCT GAAGGACCCA AGTCTCGAAG CCCAGGTCTA TGGCAACATG
    GGCATCACAA AGATGAACAT GAATGTGATG GAAGAAGCCA TCGGCTACTT CGAGCAGCAG
    CTGGCCATGC TGCAGCAGCT GAGTGGGAAC GAGTCTGTGC TTGACAGGGG CCGGGCCTAT
    GGCAACCTGG GGGACTGCTA CGAGGCCCTG GGTGACTACG AGGAAGCTAT CAAGTACTAT
    GAACAGTATT TATCTGTTGC CCAGAGTCTG AATCGCATGC AAGACCAAGC CAAGGCTTAC
    CGAGGCCTGG GAAATGGACA CAGGGCAATG GGGAGCTTGC AGCAAGCCCT GGTGTGCTTT
    GAAAAGAGGC TTGTGGTCGC CCATGAGCTC GGGGAGGCCT TTAATAAAGC CCAGGCCTAT
    GGCGAGCTGG GAAGTCTGCA CAGCCAATTA GGAAATTATG AACAAGCCAT TTCCTGCCTT
    GAACGCCAGC TGAACATTGC TAGAGAGATG AAGGACCGAG CTCTGGAAAG TGACGCGGCC
    TGTGGCCTGG GGGGTGTGTA CCAGCAGATG GGGGAGTACG ATACAGCCCT GCAGTACCAC
    CAGTTGGATC TGCAGATTGC CGAGGAGACC AACAACCCCA CGTGCCAGGG CCGGGCCTAC
    GGGAACCTGG GCCTGACCTA CGAGTCCCTG GGCACCTTTG AGAGAGCCGT GGTCTATCAG
    GAACAGCACT TGAGCATCGC TGCACAGATG AACGACTTGG TGGCCAAGAC GGTGTCTTAC
    AGCAGCCTTG GAAGGACGCA TCACGCTCTG CAGAACTACT CCCAGGCAGT CATGTACTTG
    CAGGAAGGTT TAAGGTTAGC AGAGCAGCTG GGCCGAAGAG AAGATGAAGC CAAGATTCGC
    CATGGTCTTG GCCTCTCCCT CTGGGCGAGC GGGAATCTGG AGGAGGCCCA ACATCAGCTC
    TACCGGGCAT CAGCCTTGTT TGAGACGATC CGACACGAGG CGCAGCTGAG CACAGACTAC
    AAGCTCTCCC TCTTCGACCT GCAGACGTCC TCCTACCAGG CCTTGCAGCG AGTGCTCGTC
    AGCCTCGGCC ATCATGATGA AGCTCTGGCT GTGGCAGAAA GAGGACGGAC GAGGGCATTT
    GCAGATCTCC TGGTGGAACG GCAAACAGGA CAGCAGGACT CTGACCCCTA CTCCCCGGTC
    ACTATCGATC AGATCTTAGA GATGGTTAAC GGCCAGAGGG GACTTGTGCT TTACTACTCC
    CTGGCGGCGG GCTACCTGTA CAGCTGGCTG CTTGCTCCTG GGGCAGGAAT TCTGAAGTTT
    CATGAGCACT ACCTGGGAGA CAGCACAGTG GGAAGCCCCA GTGACCCCCA GACGGGCGGC
    AGCGCGGCCC TTCCAACTGC CGCGAGCTCG GCCCTGGAGC AGCACGTTGC CAGCGTGCGG
    GAGGCCCTGG GCGTGGAGTC CTACTGCTCG AGGGCCTGTG CCAGCAGTGA GACCGAGAGT
    GAAGCTGGAG ACATCATGGA CCAGCAGTTC GAGGAGATGA ACAACAGGCT CAACTCGGTC
    ACCGACCCCA CTGGCTTTCT TCGGATGGTT CGCCGCAACA ACCTCTTTAA CAGGAGCTGC
    CAGAGCATGA CCAGCCTGGT CGGCAACACC GCGTCGCCCA TCAAGGACGG GACCTCCTCT
    CTTCCCAGGA GGCAGAGCTC ATTCGCCAGG CCGCCGCTCC GCGCCCTGTA CGACCTGCTT
    GTGGCACCCA TGGAAGGGGG CCTGATGCAC TCCAGCGGCC CAGTGGGCCG GCACCGGCAG
    CTGGTCCTGG TCCTGGAGGG GGACCTCTAC CTCATCCCGT TCGCCCTCCT GAAGGGAAGC
    TCCTCCAACG AGTACCTGTA CGAGCGCTTT GCCCTCATTG CCGTCCCCTC CATCCGCTCC
    CTCAGCCCGC AGGCCAAGTC TCACCTGAGG AAGAGCCCGC CCGCCTATTC CAGCTCCACG
    TCCATGGCGG CGGTGGTCGG CAACCCCAAG CTGCCGTCGG CGGTGATGGA CAGGTGGCTG
    TGGGGCCCGA TGCCGTCAGC CGAGGAGGAA GCCTACATGG TGTCCGAGCT GCTGGGCTGC
    CAGCCGCTGG TGGGCAGCGT GGCCACCAAA GAGCGGGTCA TGAGTGCCCT GACGCAGGCC
    GAGTGCGTCC ACTTCGCCAC CCACATCTCT TGGAAACTCT CGGCCTTGGT CCTCACGCCC
    AGTGTGGACG GCGCCCCTGC AGGCGGCAAG AGCTCCTTCG GCCACCCCTA CACCATCCCT
    GAGTCCCTGC GGGTGCAGGA CGACGCCAGC GACGGCGAGA GCATCTCAGA CTGCCCACCG
    CTGCAGGAGC TGCTGCTCAC GGCCGCCGAC ATCCTGGACC TGCGGCTGCC CGTCAAGCTG
    GTGGTGCTCG GCTCCTCCCA GGAGTCCAGC AGCAGGATCA CGGCCGATGG GGTGGTGGCG
    CTGACTCGGG CCTTCCTGGC GGCGGGTGCT CAGTGTGTCC TCGTGTCTCT GTGGCCTGTG
    CCTGTGGCCG CTTCCAAGAT GTTCCTGCAC GCCCTGTACT CGTCCCTGCT GAACGGCCTG
    AAGGCCAGTG CGGCACTGGG CGAGGCCATG AAGGTGGTGC AGAGCAGCAA GGCCTTCTCA
    CACCCCTCCA ACTGGGCAGG GTTCATGCTT ATCGGCAGCG ATGTGAAGCT CAACAGTCCC
    TCTTCGCTCA TCAGTCAGGC CCTCACCGAG ATCCTCCAGC ACCCAGAGCG CGCGCGGGAC
    GCCCTGCGCG TGCTGCTGCA CCTGGTGGAG AAGTCGCTGC AGCGCATTCA GAACGGGCAG
    CGCAACGCCA TGTACACGTC CCAGCAGAGC GTGGAGAACA AAGTGGGCGG CATCCCTGGC
    TGGCAGGCCC TCCTCACCGC TGTGGGCTTC CGGCTGGACC CCCCGGCCAG CGGCCTGCCA
    GCAGCCGTCT TCTTCCCAAC CTCCGACCCG GGCGACCGGC TCCAGCAGTG CAGCAGCACC
    GTCCAGTCCC TGCTGGGTCT GCCCAACCCT GCCCTCCAAG CCCTCTGCAA GCTCATCACG
    GCCTCGGAGA CAGGCGAGCA GCTCATCAGC CGGGCTGTTA AAAATATGGT TGGAATGCTC
    CACCAGGTGC TGGTGCAGCT GCAGGCCGGC GAGAAGGAGC AGGACTTCGC CTCAGCGCCC
    ATCCAGGTCT CCATCAGCGT CCAGCTGTGG CGGCTCCCCG GCTGCCACGA GTTCCTGGCG
    GCTCTAGGTT TTGATCTCTG TGAGGTCGGT CAGGAGGAAG TAATCCTGAA AACCGGGAAA
    CAAGCCAACC GACGGACCAT GCACTTTGCG CTCCAGGCCC TGCTCGCGCT CTTCGATTCC
    ACGGAGCTGC CCAAGCGCCT CAGCCTGGAC AGCTCGTCCT CCCTGGAGTC GCTGGCCTCG
    GCCCAGTCTG TCTCCAACGC TCTGCCCCTG GGCTTCCAGC ACCCTCCTTT CTCCCCGACC
    GGGGCAGAGA GCATAGCCTC AGACGCCATC TCTGTGTACA GCCTGAGCTC CATTGCCTCC
    TCCATGAGCT TCGTCTCCAA GCCTGACGGG GGGTCAGAAG GCGCGGTCCC TCGGGGACGG
    CAGGACTACG AGCGGTCCAA GAACACCTAC CCCCCTCGCG CTGCCCTGCC CCGGAGCCCG
    CTGTCCCCGC CTGCCCGCCC TGCAGGCGCC AAGGACGAGG AGGAGTACGA GGGGTTCTCC
    ATCATTAGCA CAGAGCCCTT GGCGGCCTCC CAGGAGGACC CACCGACGGG CTTCTCACCC
    GACCACAAGC AGTCCCTCGT GGGGACAGCG GGCGGCGTCA AAGTCTCGGT GAGCTCAAGC
    ACTCCAAACT CTCCCGTTAA AATGACTCTG ATTCCCAGCC CAAACTCACC CTTCCAGAAA
    GTTGGAAAAC TAGCAAGTTC AGATACGGGA GAATCAGACC AGTCGAGCAC AGAAACAGAT
    AGTACCGTGA AGTCCCAAGA AGAAAGTAAC TCAAAGCTTG ACCCTCAAGA GCTGGCACAG
    AAGATTCTCG AGGAGACACA AAGCCATCTC ATGGCAGTGG AGCGTCTTCA GAGGGGTGGC
    GGTCAGGGCG CCAGTTCTGA AGATGGCATC TCTGTGCCCA ACAGCGCTGC CGTCTTCCGA
    GCGTCCGAAA CCAGCGCCTT CAGCAGGCCC GCCCTCTCCC ACCAGAAGAG CCAGGCGGCG
    TTGTCGTCGT CGCCGGTCAC CATCAAACCA AAGCCCCCAG CCAGGAGCTC CTCCCTCCCC
    AAGGTGAGCT CGGGGTACAG CAGCCCCACC GCCTCCGAGG CGTCCGCCAA AGACAGCCCG
    AGCCAGCACA GCGGCCGGCC GTCGCCCGGC TCCGACTCGC AGACCTCCCT GAGCGACCAG
    CCTCTCTTCA AGCTGAAGTA CCCCAGCTCC CCCTACAGCG CTCACATCTC CAAATCTCCA
    AGGAACATGT CCCCCAGCTC AGGCCACCAG TCTCCCGCGG GAAGCGCACC CTCCCCGGCT
    CTCTCCTACT CCTCGGCGGG CTCCGCGCGG TCCAGCCCCG CCGACCACCC CGACCTCGAC
    AAGCTGAAGA TGGCGGCCAT CGACGAGAAG GTGCAGGCCG TCCACAACCT GAAGATGTTC
    TGGCAGAGCA CACCCCAGCA CTCCACGGGA CCAATGAAAA TACTCCGGGG GGCCCCTGGA
    ACCATGACTT CCAAGAGAGA TGTCCTCAGC CTATTGAATT TGTCACCTCG GCACAACAAG
    AAGGAGGAGG GCGCAGACAA GCTGGAACTC AAGGAGCTGT CTTTGCAGAG ACGTGGAGAA
    ACCCCCCCCA AAGCCCCGCC CAACGGACAC TGGCGCACGG AGACCACCTC GCTGAGCACG
    CTGCCGCTGC CCGCCCGCCC ACCTGCCCCG GCTCCCACGC GCCCTCTCAG ACTTCCTTCT
    GGAAACGGCT ACAAGTTTCT GTCCCCTGGA AGATTTTTTC CTTCCTCTAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833573.1
    CDS107..7543
    Translation

    Target ORF information:

    RefSeq Version XM_024977805.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCAGT CACCGCCGCC CGCGCCTGAG CCCACCCCGG GGTCGACCCC AGCACGGAGT 
    CGTAGACGAC GGGAGGCCGA GTCGCCGCCG GCGCCGGCTC CGATTCCTCT CTTTGGTGCT
    AAGACTATTG GCCGGAGAAG TCCTGATGGA CCAGTGCTGA GCAAAGCTGA ATTTGTTGAG
    AAAGTTCGTC AGAGTAATCA GGCCTGTCAT GATGGAGATT TCCACACAGC TATTGTTCTC
    TACAATGAAG CCCTGGCTGT TGACCCTCAG AACTGTATCT TATACAGCAA TAGATCTGCA
    GCCTATATGA AAATCCAGCA GTATGACAAG GCACTGGATG ATGCAATCAA AGCTCGACTT
    CTCAATCCCA AGTGGCCAAA GGCATACTTC CGACAGGGTG TTGCCCTCCA GTACCTCGGA
    CGTCATGCCG ATGCCCTGGC AGCCTTTGCG TCTGGGTTGG CTCAGGACCC CAAGAGTCTC
    CAGCTTCTGG TGGGGATGGT AGAAGCAGCC ATGAAATCTC CCATGAGAGA CTCCCTCGAA
    CCCACTTATC AGCAGCTTCA GAAAATGAAA CTGGACAAGA GCCCCTTTGT GGTTGTCTCT
    GTGGTTGGAC AGGAGCTCCT GACAGCTGGC CATCACGGGG CCTCTGTGGT TGTCTTAGAA
    GCCGCTCTGA AGATTGGCAC CTGCAGCCTC AAGCTGAGAG GTTCCGTTTT CTCTGCACTG
    AGCAGTGCTT ACTGGTCTCT CGGGAACACA GAGAAGAGCA CTGGATACAT GCAGCAGGAC
    TTGGATGTAG CCAAGACCTT AGGTGACCAG ACAGGAGAAT GCCGAGCTCA TGGGAACCTG
    GGCTCTGCAT TCTTCTCCAA AGGAAATTAC CGGGAGGCTC TCACTAACCA CAGGCATCAG
    TTGGTGCTTG CCATGAAACT CAAGGATCGA GAGGCAGCTT CATCAGCCCT GAGCAGTCTG
    GGCCACGTGT ACACAGCCAT TGGAGATTAC CCCAATGCAC TGGCCAGTCA CAAACAGTGT
    GTTCTTCTCG CCAAGCAATC CAAAGATGAG CTTTCTGAAG CCCGAGAACT CGGCAACATG
    GGAGCTGTGT ATATTGCCAT GGGTGACTTT GAGAATGCTG TGCAGTGCCA CGAGCAGCAT
    CTGAAGATAG CCAAGGACCT GGGGAACAAG CGAGAAGAAG CCCGGGCCTA CAGCAATCTG
    GGCAGTGCCT ATCACTACAG GAGGAACTTT GACAAGGCCA TGTCTTACCA CAATTACGTG
    CTGGAGCTGG CGCAGGAGCT GATGGAGAAG GCCATTGAGA TGCGGGCCTA CGCTGGCCTG
    GGCCACGCTG CCAGGTGCAT GCAGGATTTG GAGAGGGCCA AACAGTACCA CGAGCAGCAG
    CTGGGCATTG CTGAGGATCT CAAGGACCGG GCTGCAGAGG GGCGAGCGTC TTCCAATCTG
    GGAATCATAC ACCAGATGAA AGGTGATTAT GACACTGCAC TGAAACTCCA CAAGACCCAC
    TTGTGCATCG CCCAGGAGCT GAGTGACTAC GCGGCCCAGG GCCGCGCCTA TGGAAACATG
    GGCAACGCCT ACAATGCTCT GGGCATGTAC GACCAAGCGG TCAAGTACCA CCGGCAGGAG
    CTACAGATCT CCATGGAAGT GAACGACCGT GCCTCACAGG CATCCACGCA CGGGAACCTC
    GCTGTGGCCT ACCAGGCGCT GGGCGCCCAC GACCGGGCCC TGCAGCACTA TCAGAACCAT
    TTGAACATCG CCCGGGAGCT GCGAGACATC CAGAGCGAGG CGCGGGCTCT CAGCAACCTG
    GGCAACTTCC ACTGCTCTCG AGGGGAGTAC GTCCAGGCCG CCCCCTATTA CGAACAGTAC
    CTCCGGCTTG CTCCTGACCT TCAAGACATG GAGGGAGAAG GGAAGGTCTG CCACAACCTC
    GGCTATGCCC ATTACTGCCT TGGAAATTAC CAGGAGGCGG TAAAGTACTA TGAGCAGGAT
    CTGGCCCTGG CCAAAGACCT TCACGACAAA TTGAGTCAAG CAAAAGCTTA CTGCAACTTG
    GGCTTGGCAT TCAAGGCTCT GCTGAATTTC AGCAAAGCTG AAGAGTGTCA AAAGTACCTA
    CTGTCCCTAG CCCAGTCCCT GAATAATTCC CAGGCTAAAT TTAGAGCCCT GGGGAACCTG
    GGCGATATAT TCATCTGTAA AAAAGATATC AATGGTGCAA TAAAATTCTA TGAGCAGCAG
    CTGGGCTTGG CTCACCACGT GAAGGACAGG AGACTGGAGG CCAGTGCATA TGCAGCCCTG
    GGCACTGCAT ACCGAATGAT CCAGAAACAC GACAAGGCCT TGGGTTATCA CACACAGGAG
    CTGGAAGTAT ATCAGGAGCT GAGTGACCTG CCGGGAGAGT GCAGAGCTCA TGGGCACCTG
    GCCGCAGTCT ACATGGCCCT GGGGAAGTAC ACAATGGCTT TCAAGTGTTA CGAAGAGCAG
    CTGGATCTAG GGCAGAAGCT GAAGGACCCA AGTCTCGAAG CCCAGGTCTA TGGCAACATG
    GGCATCACAA AGATGAACAT GAATGTGATG GAAGAAGCCA TCGGCTACTT CGAGCAGCAG
    CTGGCCATGC TGCAGCAGCT GAGTGGGAAC GAGTCTGTGC TTGACAGGGG CCGGGCCTAT
    GGCAACCTGG GGGACTGCTA CGAGGCCCTG GGTGACTACG AGGAAGCTAT CAAGTACTAT
    GAACAGTATT TATCTGTTGC CCAGAGTCTG AATCGCATGC AAGACCAAGC CAAGGCTTAC
    CGAGGCCTGG GAAATGGACA CAGGGCAATG GGGAGCTTGC AGCAAGCCCT GGTGTGCTTT
    GAAAAGAGGC TTGTGGTCGC CCATGAGCTC GGGGAGGCCT TTAATAAAGC CCAGGCCTAT
    GGCGAGCTGG GAAGTCTGCA CAGCCAATTA GGAAATTATG AACAAGCCAT TTCCTGCCTT
    GAACGCCAGC TGAACATTGC TAGAGAGATG AAGGACCGAG CTCTGGAAAG TGACGCGGCC
    TGTGGCCTGG GGGGTGTGTA CCAGCAGATG GGGGAGTACG ATACAGCCCT GCAGTACCAC
    CAGTTGGATC TGCAGATTGC CGAGGAGACC AACAACCCCA CGTGCCAGGG CCGGGCCTAC
    GGGAACCTGG GCCTGACCTA CGAGTCCCTG GGCACCTTTG AGAGAGCCGT GGTCTATCAG
    GAACAGCACT TGAGCATCGC TGCACAGATG AACGACTTGG TGGCCAAGAC GGTGTCTTAC
    AGCAGCCTTG GAAGGACGCA TCACGCTCTG CAGAACTACT CCCAGGCAGT CATGTACTTG
    CAGGAAGGTT TAAGGTTAGC AGAGCAGCTG GGCCGAAGAG AAGATGAAGC CAAGATTCGC
    CATGGTCTTG GCCTCTCCCT CTGGGCGAGC GGGAATCTGG AGGAGGCCCA ACATCAGCTC
    TACCGGGCAT CAGCCTTGTT TGAGACGATC CGACACGAGG CGCAGCTGAG CACAGACTAC
    AAGCTCTCCC TCTTCGACCT GCAGACGTCC TCCTACCAGG CCTTGCAGCG AGTGCTCGTC
    AGCCTCGGCC ATCATGATGA AGCTCTGGCT GTGGCAGAAA GAGGACGGAC GAGGGCATTT
    GCAGATCTCC TGGTGGAACG GCAAACAGGA CAGCAGGACT CTGACCCCTA CTCCCCGGTC
    ACTATCGATC AGATCTTAGA GATGGTTAAC GGCCAGAGGG GACTTGTGCT TTACTACTCC
    CTGGCGGCGG GCTACCTGTA CAGCTGGCTG CTTGCTCCTG GGGCAGGAAT TCTGAAGTTT
    CATGAGCACT ACCTGGGAGA CAGCACAGTG GGAAGCCCCA GTGACCCCCA GACGGGCGGC
    AGCGCGGCCC TTCCAACTGC CGCGAGCTCG GCCCTGGAGC AGCACGTTGC CAGCGTGCGG
    GAGGCCCTGG GCGTGGAGTC CTACTGCTCG AGGGCCTGTG CCAGCAGTGA GACCGAGAGT
    GAAGCTGGAG ACATCATGGA CCAGCAGTTC GAGGAGATGA ACAACAGGCT CAACTCGGTC
    ACCGACCCCA CTGGCTTTCT TCGGATGGTT CGCCGCAACA ACCTCTTTAA CAGGAGCTGC
    CAGAGCATGA CCAGCCTGGT CGGCAACACC GCGTCGCCCA TCAAGGACGG GACCTCCTCT
    CTTCCCAGGA GGCAGAGCTC ATTCGCCAGG CCGCCGCTCC GCGCCCTGTA CGACCTGCTT
    GTGGCACCCA TGGAAGGGGG CCTGATGCAC TCCAGCGGCC CAGTGGGCCG GCACCGGCAG
    CTGGTCCTGG TCCTGGAGGG GGACCTCTAC CTCATCCCGT TCGCCCTCCT GAAGGGAAGC
    TCCTCCAACG AGTACCTGTA CGAGCGCTTT GCCCTCATTG CCGTCCCCTC CATCCGCTCC
    CTCAGCCCGC AGGCCAAGTC TCACCTGAGG AAGAGCCCGC CCGCCTATTC CAGCTCCACG
    TCCATGGCGG CGGTGGTCGG CAACCCCAAG CTGCCGTCGG CGGTGATGGA CAGGTGGCTG
    TGGGGCCCGA TGCCGTCAGC CGAGGAGGAA GCCTACATGG TGTCCGAGCT GCTGGGCTGC
    CAGCCGCTGG TGGGCAGCGT GGCCACCAAA GAGCGGGTCA TGAGTGCCCT GACGCAGGCC
    GAGTGCGTCC ACTTCGCCAC CCACATCTCT TGGAAACTCT CGGCCTTGGT CCTCACGCCC
    AGTGTGGACG GCGCCCCTGC AGGCGGCAAG AGCTCCTTCG GCCACCCCTA CACCATCCCT
    GAGTCCCTGC GGGTGCAGGA CGACGCCAGC GACGGCGAGA GCATCTCAGA CTGCCCACCG
    CTGCAGGAGC TGCTGCTCAC GGCCGCCGAC ATCCTGGACC TGCGGCTGCC CGTCAAGCTG
    GTGGTGCTCG GCTCCTCCCA GGAGTCCAGC AGCAGGATCA CGGCCGATGG GGTGGTGGCG
    CTGACTCGGG CCTTCCTGGC GGCGGGTGCT CAGTGTGTCC TCGTGTCTCT GTGGCCTGTG
    CCTGTGGCCG CTTCCAAGAT GTTCCTGCAC GCCCTGTACT CGTCCCTGCT GAACGGCCTG
    AAGGCCAGTG CGGCACTGGG CGAGGCCATG AAGGTGGTGC AGAGCAGCAA GGCCTTCTCA
    CACCCCTCCA ACTGGGCAGG GTTCATGCTT ATCGGCAGCG ATGTGAAGCT CAACAGTCCC
    TCTTCGCTCA TCAGTCAGGC CCTCACCGAG ATCCTCCAGC ACCCAGAGCG CGCGCGGGAC
    GCCCTGCGCG TGCTGCTGCA CCTGGTGGAG AAGTCGCTGC AGCGCATTCA GAACGGGCAG
    CGCAACGCCA TGTACACGTC CCAGCAGAGC GTGGAGAACA AAGTGGGCGG CATCCCTGGC
    TGGCAGGCCC TCCTCACCGC TGTGGGCTTC CGGCTGGACC CCCCGGCCAG CGGCCTGCCA
    GCAGCCGTCT TCTTCCCAAC CTCCGACCCG GGCGACCGGC TCCAGCAGTG CAGCAGCACC
    GTCCAGTCCC TGCTGGGTCT GCCCAACCCT GCCCTCCAAG CCCTCTGCAA GCTCATCACG
    GCCTCGGAGA CAGGCGAGCA GCTCATCAGC CGGGCTGTTA AAAATATGGT TGGAATGCTC
    CACCAGGTGC TGGTGCAGCT GCAGGCCGGC GAGAAGGAGC AGGACTTCGC CTCAGCGCCC
    ATCCAGGTCT CCATCAGCGT CCAGCTGTGG CGGCTCCCCG GCTGCCACGA GTTCCTGGCG
    GCTCTAGGTT TTGATCTCTG TGAGGTCGGT CAGGAGGAAG TAATCCTGAA AACCGGGAAA
    CAAGCCAACC GACGGACCAT GCACTTTGCG CTCCAGGCCC TGCTCGCGCT CTTCGATTCC
    ACGGAGCTGC CCAAGCGCCT CAGCCTGGAC AGCTCGTCCT CCCTGGAGTC GCTGGCCTCG
    GCCCAGTCTG TCTCCAACGC TCTGCCCCTG GGCTTCCAGC ACCCTCCTTT CTCCCCGACC
    GGGGCAGAGA GCATAGCCTC AGACGCCATC TCTGTGTACA GCCTGAGCTC CATTGCCTCC
    TCCATGAGCT TCGTCTCCAA GCCTGACGGG GGGTCAGAAG GCGCGGTCCC TCGGGGACGG
    CAGGACTACG AGCGGTCCAA GAACACCTAC CCCCCTCGCG CTGCCCTGCC CCGGAGCCCG
    CTGTCCCCGC CTGCCCGCCC TGCAGGCGCC AAGGACGAGG AGGAGTACGA GGGGTTCTCC
    ATCATTAGCA CAGAGCCCTT GGCGGCCTCC CAGGAGGACC CACCGACGGG CTTCTCACCC
    GACCACAAGC AGTCCCTCGT GGGGACAGCG GGCGGCGTCA AAGTCTCGGT GAGCTCAAGC
    ACTCCAAACT CTCCCGTTAA AATGACTCTG ATTCCCAGCC CAAACTCACC CTTCCAGAAA
    GTTGGAAAAC TAGCAAGTTC AGATACGGGA GAATCAGACC AGTCGAGCAC AGAAACAGAT
    AGTACCGTGA AGTCCCAAGA AGAAAGTAAC TCAAAGCTTG ACCCTCAAGA GCTGGCACAG
    AAGATTCTCG AGGAGACACA AAGCCATCTC ATGGCAGTGG AGCGTCTTCA GAGGGGTGGC
    GGTCAGGGCG CCAGTTCTGA AGATGGCATC TCTGTGCCCA ACAGCGCTGC CGTCTTCCGA
    GCGTCCGAAA CCAGCGCCTT CAGCAGGCCC GCCCTCTCCC ACCAGAAGAG CCAGGCGGCG
    TTGTCGTCGT CGCCGGTCAC CATCAAACCA AAGCCCCCAG CCAGGAGCTC CTCCCTCCCC
    AAGGTGAGCT CGGGGTACAG CAGCCCCACC GCCTCCGAGG CGTCCGCCAA AGACAGCCCG
    AGCCAGCACA GCGGCCGGCC GTCGCCCGGC TCCGACTCGC AGACCTCCCT GAGCGACCAG
    CCTCTCTTCA AGCTGAAGTA CCCCAGCTCC CCCTACAGCG CTCACATCTC CAAATCTCCA
    AGGAACATGT CCCCCAGCTC AGGCCACCAG TCTCCCGCGG GAAGCGCACC CTCCCCGGCT
    CTCTCCTACT CCTCGGCGGG CTCCGCGCGG TCCAGCCCCG CCGACCACCC CGACCTCGAC
    AAGCTGAAGA TGGCGGCCAT CGACGAGAAG GTGCAGGCCG TCCACAACCT GAAGATGTTC
    TGGCAGAGCA CACCCCAGCA CTCCACGGGA CCAATGAAAA TACTCCGGGG GGCCCCTGGA
    ACCATGACTT CCAAGAGAGA TGTCCTCAGC CTATTGAATT TGTCACCTCG GCACAACAAG
    AAGGAGGAGG GCGCAGACAA GCTGGAACTC AAGGAGCTGT CTTTGCAGAG ACGTGGAGAA
    ACCCCCCCCA AAGCCCCGCC CAACGGACAC TGGCGCACGG AGACCACCTC GCTGAGCACG
    CTGCCGCTGC CCGCCCGCCC ACCTGCCCCG GCTCCCACGC GCCCTCTCAG ACTTCCTTCT
    GGAAACGGCT ACAAGTTTCT GTCCCCTGGA AGATTTTTTC CTTCCTCTAA ATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224679
    Clone ID Related Accession (Same CDS sequence) XM_024977806.1
    Accession Version XM_024977806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCAGT CACCGCCGCC CGCGCCTGAG CCCACCCCGG GGTCGACCCC AGCACGGAGT 
    CGTAGACGAC GGGAGGCCGA GTCGCCGCCG GCGCCGGCTC CGATTCCTCT CTTTGGTGCT
    AAGACTATTG GCCGGAGAAG TCCTGATGGA CCAGTGCTGA GCAAAGCTGA ATTTGTTGAG
    AAAGTTCGTC AGAGTAATCA GGCCTGTCAT GATGGAGATT TCCACACAGC TATTGTTCTC
    TACAATGAAG CCCTGGCTGT TGACCCTCAG AACTGTATCT TATACAGCAA TAGATCTGCA
    GCCTATATGA AAATCCAGCA GTATGACAAG GCACTGGATG ATGCAATCAA AGCTCGACTT
    CTCAATCCCA AGTGGCCAAA GGCATACTTC CGACAGGGTG TTGCCCTCCA GTACCTCGGA
    CGTCATGCCG ATGCCCTGGC AGCCTTTGCG TCTGGGTTGG CTCAGGACCC CAAGAGTCTC
    CAGCTTCTGG TGGGGATGGT AGAAGCAGCC ATGAAATCTC CCATGAGAGA CTCCCTCGAA
    CCCACTTATC AGCAGCTTCA GAAAATGAAA CTGGACAAGA GCCCCTTTGT GGTTGTCTCT
    GTGGTTGGAC AGGAGCTCCT GACAGCTGGC CATCACGGGG CCTCTGTGGT TGTCTTAGAA
    GCCGCTCTGA AGATTGGCAC CTGCAGCCTC AAGCTGAGAG GTTCCGTTTT CTCTGCACTG
    AGCAGTGCTT ACTGGTCTCT CGGGAACACA GAGAAGAGCA CTGGATACAT GCAGCAGGAC
    TTGGATGTAG CCAAGACCTT AGGTGACCAG ACAGGAGAAT GCCGAGCTCA TGGGAACCTG
    GGCTCTGCAT TCTTCTCCAA AGGAAATTAC CGGGAGGCTC TCACTAACCA CAGGCATCAG
    TTGGTGCTTG CCATGAAACT CAAGGATCGA GAGGCAGCTT CATCAGCCCT GAGCAGTCTG
    GGCCACGTGT ACACAGCCAT TGGAGATTAC CCCAATGCAC TGGCCAGTCA CAAACAGTGT
    GTTCTTCTCG CCAAGCAATC CAAAGATGAG CTTTCTGAAG CCCGAGAACT CGGCAACATG
    GGAGCTGTGT ATATTGCCAT GGGTGACTTT GAGAATGCTG TGCAGTGCCA CGAGCAGCAT
    CTGAAGATAG CCAAGGACCT GGGGAACAAG CGAGAAGAAG CCCGGGCCTA CAGCAATCTG
    GGCAGTGCCT ATCACTACAG GAGGAACTTT GACAAGGCCA TGTCTTACCA CAATTACGTG
    CTGGAGCTGG CGCAGGAGCT GATGGAGAAG GCCATTGAGA TGCGGGCCTA CGCTGGCCTG
    GGCCACGCTG CCAGGTGCAT GCAGGATTTG GAGAGGGCCA AACAGTACCA CGAGCAGCAG
    CTGGGCATTG CTGAGGATCT CAAGGACCGG GCTGCAGAGG GGCGAGCGTC TTCCAATCTG
    GGAATCATAC ACCAGATGAA AGGTGATTAT GACACTGCAC TGAAACTCCA CAAGACCCAC
    TTGTGCATCG CCCAGGAGCT GAGTGACTAC GCGGCCCAGG GCCGCGCCTA TGGAAACATG
    GGCAACGCCT ACAATGCTCT GGGCATGTAC GACCAAGCGG TCAAGTACCA CCGGCAGGAG
    CTACAGATCT CCATGGAAGT GAACGACCGT GCCTCACAGG CATCCACGCA CGGGAACCTC
    GCTGTGGCCT ACCAGGCGCT GGGCGCCCAC GACCGGGCCC TGCAGCACTA TCAGAACCAT
    TTGAACATCG CCCGGGAGCT GCGAGACATC CAGAGCGAGG CGCGGGCTCT CAGCAACCTG
    GGCAACTTCC ACTGCTCTCG AGGGGAGTAC GTCCAGGCCG CCCCCTATTA CGAACAGTAC
    CTCCGGCTTG CTCCTGACCT TCAAGACATG GAGGGAGAAG GGAAGGTCTG CCACAACCTC
    GGCTATGCCC ATTACTGCCT TGGAAATTAC CAGGAGGCGG TAAAGTACTA TGAGCAGGAT
    CTGGCCCTGG CCAAAGACCT TCACGACAAA TTGAGTCAAG CAAAAGCTTA CTGCAACTTG
    GGCTTGGCAT TCAAGGCTCT GCTGAATTTC AGCAAAGCTG AAGAGTGTCA AAAGTACCTA
    CTGTCCCTAG CCCAGTCCCT GAATAATTCC CAGGCTAAAT TTAGAGCCCT GGGGAACCTG
    GGCGATATAT TCATCTGTAA AAAAGATATC AATGGTGCAA TAAAATTCTA TGAGCAGCAG
    CTGGGCTTGG CTCACCACGT GAAGGACAGG AGACTGGAGG CCAGTGCATA TGCAGCCCTG
    GGCACTGCAT ACCGAATGAT CCAGAAACAC GACAAGGCCT TGGGTTATCA CACACAGGAG
    CTGGAAGTAT ATCAGGAGCT GAGTGACCTG CCGGGAGAGT GCAGAGCTCA TGGGCACCTG
    GCCGCAGTCT ACATGGCCCT GGGGAAGTAC ACAATGGCTT TCAAGTGTTA CGAAGAGCAG
    CTGGATCTAG GGCAGAAGCT GAAGGACCCA AGTCTCGAAG CCCAGGTCTA TGGCAACATG
    GGCATCACAA AGATGAACAT GAATGTGATG GAAGAAGCCA TCGGCTACTT CGAGCAGCAG
    CTGGCCATGC TGCAGCAGCT GAGTGGGAAC GAGTCTGTGC TTGACAGGGG CCGGGCCTAT
    GGCAACCTGG GGGACTGCTA CGAGGCCCTG GGTGACTACG AGGAAGCTAT CAAGTACTAT
    GAACAGTATT TATCTGTTGC CCAGAGTCTG AATCGCATGC AAGACCAAGC CAAGGCTTAC
    CGAGGCCTGG GAAATGGACA CAGGGCAATG GGGAGCTTGC AGCAAGCCCT GGTGTGCTTT
    GAAAAGAGGC TTGTGGTCGC CCATGAGCTC GGGGAGGCCT TTAATAAAGC CCAGGCCTAT
    GGCGAGCTGG GAAGTCTGCA CAGCCAATTA GGAAATTATG AACAAGCCAT TTCCTGCCTT
    GAACGCCAGC TGAACATTGC TAGAGAGATG AAGGACCGAG CTCTGGAAAG TGACGCGGCC
    TGTGGCCTGG GGGGTGTGTA CCAGCAGATG GGGGAGTACG ATACAGCCCT GCAGTACCAC
    CAGTTGGATC TGCAGATTGC CGAGGAGACC AACAACCCCA CGTGCCAGGG CCGGGCCTAC
    GGGAACCTGG GCCTGACCTA CGAGTCCCTG GGCACCTTTG AGAGAGCCGT GGTCTATCAG
    GAACAGCACT TGAGCATCGC TGCACAGATG AACGACTTGG TGGCCAAGAC GGTGTCTTAC
    AGCAGCCTTG GAAGGACGCA TCACGCTCTG CAGAACTACT CCCAGGCAGT CATGTACTTG
    CAGGAAGGTT TAAGGTTAGC AGAGCAGCTG GGCCGAAGAG AAGATGAAGC CAAGATTCGC
    CATGGTCTTG GCCTCTCCCT CTGGGCGAGC GGGAATCTGG AGGAGGCCCA ACATCAGCTC
    TACCGGGCAT CAGCCTTGTT TGAGACGATC CGACACGAGG CGCAGCTGAG CACAGACTAC
    AAGCTCTCCC TCTTCGACCT GCAGACGTCC TCCTACCAGG CCTTGCAGCG AGTGCTCGTC
    AGCCTCGGCC ATCATGATGA AGCTCTGGCT GTGGCAGAAA GAGGACGGAC GAGGGCATTT
    GCAGATCTCC TGGTGGAACG GCAAACAGGA CAGCAGGACT CTGACCCCTA CTCCCCGGTC
    ACTATCGATC AGATCTTAGA GATGGTTAAC GGCCAGAGGG GACTTGTGCT TTACTACTCC
    CTGGCGGCGG GCTACCTGTA CAGCTGGCTG CTTGCTCCTG GGGCAGGAAT TCTGAAGTTT
    CATGAGCACT ACCTGGGAGA CAGCACAGTG GGAAGCCCCA GTGACCCCCA GACGGGCGGC
    AGCGCGGCCC TTCCAACTGC CGCGAGCTCG GCCCTGGAGC AGCACGTTGC CAGCGTGCGG
    GAGGCCCTGG GCGTGGAGTC CTACTGCTCG AGGGCCTGTG CCAGCAGTGA GACCGAGAGT
    GAAGCTGGAG ACATCATGGA CCAGCAGTTC GAGGAGATGA ACAACAGGCT CAACTCGGTC
    ACCGACCCCA CTGGCTTTCT TCGGATGGTT CGCCGCAACA ACCTCTTTAA CAGGAGCTGC
    CAGAGCATGA CCAGCCTGGT CGGCAACACC GCGTCGCCCA TCAAGGACGG GACCTCCTCT
    CTTCCCAGGA GGCAGAGCTC ATTCGCCAGG CCGCCGCTCC GCGCCCTGTA CGACCTGCTT
    GTGGCACCCA TGGAAGGGGG CCTGATGCAC TCCAGCGGCC CAGTGGGCCG GCACCGGCAG
    CTGGTCCTGG TCCTGGAGGG GGACCTCTAC CTCATCCCGT TCGCCCTCCT GAAGGGAAGC
    TCCTCCAACG AGTACCTGTA CGAGCGCTTT GCCCTCATTG CCGTCCCCTC CATCCGCTCC
    CTCAGCCCGC AGGCCAAGTC TCACCTGAGG AAGAGCCCGC CCGCCTATTC CAGCTCCACG
    TCCATGGCGG CGGTGGTCGG CAACCCCAAG CTGCCGTCGG CGGTGATGGA CAGGTGGCTG
    TGGGGCCCGA TGCCGTCAGC CGAGGAGGAA GCCTACATGG TGTCCGAGCT GCTGGGCTGC
    CAGCCGCTGG TGGGCAGCGT GGCCACCAAA GAGCGGGTCA TGAGTGCCCT GACGCAGGCC
    GAGTGCGTCC ACTTCGCCAC CCACATCTCT TGGAAACTCT CGGCCTTGGT CCTCACGCCC
    AGTGTGGACG GCGCCCCTGC AGGCGGCAAG AGCTCCTTCG GCCACCCCTA CACCATCCCT
    GAGTCCCTGC GGGTGCAGGA CGACGCCAGC GACGGCGAGA GCATCTCAGA CTGCCCACCG
    CTGCAGGAGC TGCTGCTCAC GGCCGCCGAC ATCCTGGACC TGCGGCTGCC CGTCAAGCTG
    GTGGTGCTCG GCTCCTCCCA GGAGTCCAGC AGCAGGATCA CGGCCGATGG GGTGGTGGCG
    CTGACTCGGG CCTTCCTGGC GGCGGGTGCT CAGTGTGTCC TCGTGTCTCT GTGGCCTGTG
    CCTGTGGCCG CTTCCAAGAT GTTCCTGCAC GCCCTGTACT CGTCCCTGCT GAACGGCCTG
    AAGGCCAGTG CGGCACTGGG CGAGGCCATG AAGGTGGTGC AGAGCAGCAA GGCCTTCTCA
    CACCCCTCCA ACTGGGCAGG GTTCATGCTT ATCGGCAGCG ATGTGAAGCT CAACAGTCCC
    TCTTCGCTCA TCAGTCAGGC CCTCACCGAG ATCCTCCAGC ACCCAGAGCG CGCGCGGGAC
    GCCCTGCGCG TGCTGCTGCA CCTGGTGGAG AAGTCGCTGC AGCGCATTCA GAACGGGCAG
    CGCAACGCCA TGTACACGTC CCAGCAGAGC GTGGAGAACA AAGTGGGCGG CATCCCTGGC
    TGGCAGGCCC TCCTCACCGC TGTGGGCTTC CGGCTGGACC CCCCGGCCAG CGGCCTGCCA
    GCAGCCGTCT TCTTCCCAAC CTCCGACCCG GGCGACCGGC TCCAGCAGTG CAGCAGCACC
    GTCCAGTCCC TGCTGGGTCT GCCCAACCCT GCCCTCCAAG CCCTCTGCAA GCTCATCACG
    GCCTCGGAGA CAGGCGAGCA GCTCATCAGC CGGCTCCACC AGGTGCTGGT GCAGCTGCAG
    GCCGGCGAGA AGGAGCAGGA CTTCGCCTCA GCGCCCATCC AGGTCTCCAT CAGCGTCCAG
    CTGTGGCGGC TCCCCGGCTG CCACGAGTTC CTGGCGGCTC TAGGTTTTGA TCTCTGTGAG
    GTCGGTCAGG AGGAAGTAAT CCTGAAAACC GGGAAACAAG CCAACCGACG GACCATGCAC
    TTTGCGCTCC AGGCCCTGCT CGCGCTCTTC GATTCCACGG AGCTGCCCAA GCGCCTCAGC
    CTGGACAGCT CGTCCTCCCT GGAGTCGCTG GCCTCGGCCC AGTCTGTCTC CAACGCTCTG
    CCCCTGGGCT TCCAGCACCC TCCTTTCTCC CCGACCGGGG CAGAGAGCAT AGCCTCAGAC
    GCCATCTCTG TGTACAGCCT GAGCTCCATT GCCTCCTCCA TGAGCTTCGT CTCCAAGCCT
    GACGGGGGGT CAGAAGGCGC GGTCCCTCGG GGACGGCAGG ACTACGAGCG GTCCAAGAAC
    ACCTACCCCC CTCGCGCTGC CCTGCCCCGG AGCCCGCTGT CCCCGCCTGC CCGCCCTGCA
    GGCGCCAAGG ACGAGGAGGA GTACGAGGGG TTCTCCATCA TTAGCACAGA GCCCTTGGCG
    GCCTCCCAGG AGGACCCACC GACGGGCTTC TCACCCGACC ACAAGCAGTC CCTCGTGGGG
    ACAGCGGGCG GCGTCAAAGT CTCGGTGAGC TCAAGCACTC CAAACTCTCC CGTTAAAATG
    ACTCTGATTC CCAGCCCAAA CTCACCCTTC CAGAAAGTTG GAAAACTAGC AAGTTCAGAT
    ACGGGAGAAT CAGACCAGTC GAGCACAGAA ACAGATAGTA CCGTGAAGTC CCAAGAAGAA
    AGTAACTCAA AGCTTGACCC TCAAGAGCTG GCACAGAAGA TTCTCGAGGA GACACAAAGC
    CATCTCATGG CAGTGGAGCG TCTTCAGAGG GGTGGCGGTC AGGGCGCCAG TTCTGAAGAT
    GGCATCTCTG TGCCCAACAG CGCTGCCGTC TTCCGAGCGT CCGAAACCAG CGCCTTCAGC
    AGGCCCGCCC TCTCCCACCA GAAGAGCCAG GCGGCGTTGT CGTCGTCGCC GGTCACCATC
    AAACCAAAGC CCCCAGCCAG GAGCTCCTCC CTCCCCAAGG TGAGCTCGGG GTACAGCAGC
    CCCACCGCCT CCGAGGCGTC CGCCAAAGAC AGCCCGAGCC AGCACAGCGG CCGGCCGTCG
    CCCGGCTCCG ACTCGCAGAC CTCCCTGAGC GACCAGCCTC TCTTCAAGCT GAAGTACCCC
    AGCTCCCCCT ACAGCGCTCA CATCTCCAAA TCTCCAAGGA ACATGTCCCC CAGCTCAGGC
    CACCAGTCTC CCGCGGGAAG CGCACCCTCC CCGGCTCTCT CCTACTCCTC GGCGGGCTCC
    GCGCGGTCCA GCCCCGCCGA CCACCCCGAC CTCGACAAGC TGAAGATGGC GGCCATCGAC
    GAGAAGGTGC AGGCCGTCCA CAACCTGAAG ATGTTCTGGC AGAGCACACC CCAGCACTCC
    ACGGGACCAA TGAAAATACT CCGGGGGGCC CCTGGAACCA TGACTTCCAA GAGAGATGTC
    CTCAGCCTAT TGAATTTGTC ACCTCGGCAC AACAAGAAGG AGGAGGGCGC AGACAAGCTG
    GAACTCAAGG AGCTGTCTTT GCAGAGACGT GGAGAAACCC CCCCCAAAGC CCCGCCCAAC
    GGACACTGGC GCACGGAGAC CACCTCGCTG AGCACGCTGC CGCTGCCCGC CCGCCCACCT
    GCCCCGGCTC CCACGCGCCC TCTCAGACTT CCTTCTGGAA ACGGCTACAA GTTTCTGTCC
    CCTGGAAGAT TTTTTCCTTC CTCTAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833574.1
    CDS107..7519
    Translation

    Target ORF information:

    RefSeq Version XM_024977806.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCAGT CACCGCCGCC CGCGCCTGAG CCCACCCCGG GGTCGACCCC AGCACGGAGT 
    CGTAGACGAC GGGAGGCCGA GTCGCCGCCG GCGCCGGCTC CGATTCCTCT CTTTGGTGCT
    AAGACTATTG GCCGGAGAAG TCCTGATGGA CCAGTGCTGA GCAAAGCTGA ATTTGTTGAG
    AAAGTTCGTC AGAGTAATCA GGCCTGTCAT GATGGAGATT TCCACACAGC TATTGTTCTC
    TACAATGAAG CCCTGGCTGT TGACCCTCAG AACTGTATCT TATACAGCAA TAGATCTGCA
    GCCTATATGA AAATCCAGCA GTATGACAAG GCACTGGATG ATGCAATCAA AGCTCGACTT
    CTCAATCCCA AGTGGCCAAA GGCATACTTC CGACAGGGTG TTGCCCTCCA GTACCTCGGA
    CGTCATGCCG ATGCCCTGGC AGCCTTTGCG TCTGGGTTGG CTCAGGACCC CAAGAGTCTC
    CAGCTTCTGG TGGGGATGGT AGAAGCAGCC ATGAAATCTC CCATGAGAGA CTCCCTCGAA
    CCCACTTATC AGCAGCTTCA GAAAATGAAA CTGGACAAGA GCCCCTTTGT GGTTGTCTCT
    GTGGTTGGAC AGGAGCTCCT GACAGCTGGC CATCACGGGG CCTCTGTGGT TGTCTTAGAA
    GCCGCTCTGA AGATTGGCAC CTGCAGCCTC AAGCTGAGAG GTTCCGTTTT CTCTGCACTG
    AGCAGTGCTT ACTGGTCTCT CGGGAACACA GAGAAGAGCA CTGGATACAT GCAGCAGGAC
    TTGGATGTAG CCAAGACCTT AGGTGACCAG ACAGGAGAAT GCCGAGCTCA TGGGAACCTG
    GGCTCTGCAT TCTTCTCCAA AGGAAATTAC CGGGAGGCTC TCACTAACCA CAGGCATCAG
    TTGGTGCTTG CCATGAAACT CAAGGATCGA GAGGCAGCTT CATCAGCCCT GAGCAGTCTG
    GGCCACGTGT ACACAGCCAT TGGAGATTAC CCCAATGCAC TGGCCAGTCA CAAACAGTGT
    GTTCTTCTCG CCAAGCAATC CAAAGATGAG CTTTCTGAAG CCCGAGAACT CGGCAACATG
    GGAGCTGTGT ATATTGCCAT GGGTGACTTT GAGAATGCTG TGCAGTGCCA CGAGCAGCAT
    CTGAAGATAG CCAAGGACCT GGGGAACAAG CGAGAAGAAG CCCGGGCCTA CAGCAATCTG
    GGCAGTGCCT ATCACTACAG GAGGAACTTT GACAAGGCCA TGTCTTACCA CAATTACGTG
    CTGGAGCTGG CGCAGGAGCT GATGGAGAAG GCCATTGAGA TGCGGGCCTA CGCTGGCCTG
    GGCCACGCTG CCAGGTGCAT GCAGGATTTG GAGAGGGCCA AACAGTACCA CGAGCAGCAG
    CTGGGCATTG CTGAGGATCT CAAGGACCGG GCTGCAGAGG GGCGAGCGTC TTCCAATCTG
    GGAATCATAC ACCAGATGAA AGGTGATTAT GACACTGCAC TGAAACTCCA CAAGACCCAC
    TTGTGCATCG CCCAGGAGCT GAGTGACTAC GCGGCCCAGG GCCGCGCCTA TGGAAACATG
    GGCAACGCCT ACAATGCTCT GGGCATGTAC GACCAAGCGG TCAAGTACCA CCGGCAGGAG
    CTACAGATCT CCATGGAAGT GAACGACCGT GCCTCACAGG CATCCACGCA CGGGAACCTC
    GCTGTGGCCT ACCAGGCGCT GGGCGCCCAC GACCGGGCCC TGCAGCACTA TCAGAACCAT
    TTGAACATCG CCCGGGAGCT GCGAGACATC CAGAGCGAGG CGCGGGCTCT CAGCAACCTG
    GGCAACTTCC ACTGCTCTCG AGGGGAGTAC GTCCAGGCCG CCCCCTATTA CGAACAGTAC
    CTCCGGCTTG CTCCTGACCT TCAAGACATG GAGGGAGAAG GGAAGGTCTG CCACAACCTC
    GGCTATGCCC ATTACTGCCT TGGAAATTAC CAGGAGGCGG TAAAGTACTA TGAGCAGGAT
    CTGGCCCTGG CCAAAGACCT TCACGACAAA TTGAGTCAAG CAAAAGCTTA CTGCAACTTG
    GGCTTGGCAT TCAAGGCTCT GCTGAATTTC AGCAAAGCTG AAGAGTGTCA AAAGTACCTA
    CTGTCCCTAG CCCAGTCCCT GAATAATTCC CAGGCTAAAT TTAGAGCCCT GGGGAACCTG
    GGCGATATAT TCATCTGTAA AAAAGATATC AATGGTGCAA TAAAATTCTA TGAGCAGCAG
    CTGGGCTTGG CTCACCACGT GAAGGACAGG AGACTGGAGG CCAGTGCATA TGCAGCCCTG
    GGCACTGCAT ACCGAATGAT CCAGAAACAC GACAAGGCCT TGGGTTATCA CACACAGGAG
    CTGGAAGTAT ATCAGGAGCT GAGTGACCTG CCGGGAGAGT GCAGAGCTCA TGGGCACCTG
    GCCGCAGTCT ACATGGCCCT GGGGAAGTAC ACAATGGCTT TCAAGTGTTA CGAAGAGCAG
    CTGGATCTAG GGCAGAAGCT GAAGGACCCA AGTCTCGAAG CCCAGGTCTA TGGCAACATG
    GGCATCACAA AGATGAACAT GAATGTGATG GAAGAAGCCA TCGGCTACTT CGAGCAGCAG
    CTGGCCATGC TGCAGCAGCT GAGTGGGAAC GAGTCTGTGC TTGACAGGGG CCGGGCCTAT
    GGCAACCTGG GGGACTGCTA CGAGGCCCTG GGTGACTACG AGGAAGCTAT CAAGTACTAT
    GAACAGTATT TATCTGTTGC CCAGAGTCTG AATCGCATGC AAGACCAAGC CAAGGCTTAC
    CGAGGCCTGG GAAATGGACA CAGGGCAATG GGGAGCTTGC AGCAAGCCCT GGTGTGCTTT
    GAAAAGAGGC TTGTGGTCGC CCATGAGCTC GGGGAGGCCT TTAATAAAGC CCAGGCCTAT
    GGCGAGCTGG GAAGTCTGCA CAGCCAATTA GGAAATTATG AACAAGCCAT TTCCTGCCTT
    GAACGCCAGC TGAACATTGC TAGAGAGATG AAGGACCGAG CTCTGGAAAG TGACGCGGCC
    TGTGGCCTGG GGGGTGTGTA CCAGCAGATG GGGGAGTACG ATACAGCCCT GCAGTACCAC
    CAGTTGGATC TGCAGATTGC CGAGGAGACC AACAACCCCA CGTGCCAGGG CCGGGCCTAC
    GGGAACCTGG GCCTGACCTA CGAGTCCCTG GGCACCTTTG AGAGAGCCGT GGTCTATCAG
    GAACAGCACT TGAGCATCGC TGCACAGATG AACGACTTGG TGGCCAAGAC GGTGTCTTAC
    AGCAGCCTTG GAAGGACGCA TCACGCTCTG CAGAACTACT CCCAGGCAGT CATGTACTTG
    CAGGAAGGTT TAAGGTTAGC AGAGCAGCTG GGCCGAAGAG AAGATGAAGC CAAGATTCGC
    CATGGTCTTG GCCTCTCCCT CTGGGCGAGC GGGAATCTGG AGGAGGCCCA ACATCAGCTC
    TACCGGGCAT CAGCCTTGTT TGAGACGATC CGACACGAGG CGCAGCTGAG CACAGACTAC
    AAGCTCTCCC TCTTCGACCT GCAGACGTCC TCCTACCAGG CCTTGCAGCG AGTGCTCGTC
    AGCCTCGGCC ATCATGATGA AGCTCTGGCT GTGGCAGAAA GAGGACGGAC GAGGGCATTT
    GCAGATCTCC TGGTGGAACG GCAAACAGGA CAGCAGGACT CTGACCCCTA CTCCCCGGTC
    ACTATCGATC AGATCTTAGA GATGGTTAAC GGCCAGAGGG GACTTGTGCT TTACTACTCC
    CTGGCGGCGG GCTACCTGTA CAGCTGGCTG CTTGCTCCTG GGGCAGGAAT TCTGAAGTTT
    CATGAGCACT ACCTGGGAGA CAGCACAGTG GGAAGCCCCA GTGACCCCCA GACGGGCGGC
    AGCGCGGCCC TTCCAACTGC CGCGAGCTCG GCCCTGGAGC AGCACGTTGC CAGCGTGCGG
    GAGGCCCTGG GCGTGGAGTC CTACTGCTCG AGGGCCTGTG CCAGCAGTGA GACCGAGAGT
    GAAGCTGGAG ACATCATGGA CCAGCAGTTC GAGGAGATGA ACAACAGGCT CAACTCGGTC
    ACCGACCCCA CTGGCTTTCT TCGGATGGTT CGCCGCAACA ACCTCTTTAA CAGGAGCTGC
    CAGAGCATGA CCAGCCTGGT CGGCAACACC GCGTCGCCCA TCAAGGACGG GACCTCCTCT
    CTTCCCAGGA GGCAGAGCTC ATTCGCCAGG CCGCCGCTCC GCGCCCTGTA CGACCTGCTT
    GTGGCACCCA TGGAAGGGGG CCTGATGCAC TCCAGCGGCC CAGTGGGCCG GCACCGGCAG
    CTGGTCCTGG TCCTGGAGGG GGACCTCTAC CTCATCCCGT TCGCCCTCCT GAAGGGAAGC
    TCCTCCAACG AGTACCTGTA CGAGCGCTTT GCCCTCATTG CCGTCCCCTC CATCCGCTCC
    CTCAGCCCGC AGGCCAAGTC TCACCTGAGG AAGAGCCCGC CCGCCTATTC CAGCTCCACG
    TCCATGGCGG CGGTGGTCGG CAACCCCAAG CTGCCGTCGG CGGTGATGGA CAGGTGGCTG
    TGGGGCCCGA TGCCGTCAGC CGAGGAGGAA GCCTACATGG TGTCCGAGCT GCTGGGCTGC
    CAGCCGCTGG TGGGCAGCGT GGCCACCAAA GAGCGGGTCA TGAGTGCCCT GACGCAGGCC
    GAGTGCGTCC ACTTCGCCAC CCACATCTCT TGGAAACTCT CGGCCTTGGT CCTCACGCCC
    AGTGTGGACG GCGCCCCTGC AGGCGGCAAG AGCTCCTTCG GCCACCCCTA CACCATCCCT
    GAGTCCCTGC GGGTGCAGGA CGACGCCAGC GACGGCGAGA GCATCTCAGA CTGCCCACCG
    CTGCAGGAGC TGCTGCTCAC GGCCGCCGAC ATCCTGGACC TGCGGCTGCC CGTCAAGCTG
    GTGGTGCTCG GCTCCTCCCA GGAGTCCAGC AGCAGGATCA CGGCCGATGG GGTGGTGGCG
    CTGACTCGGG CCTTCCTGGC GGCGGGTGCT CAGTGTGTCC TCGTGTCTCT GTGGCCTGTG
    CCTGTGGCCG CTTCCAAGAT GTTCCTGCAC GCCCTGTACT CGTCCCTGCT GAACGGCCTG
    AAGGCCAGTG CGGCACTGGG CGAGGCCATG AAGGTGGTGC AGAGCAGCAA GGCCTTCTCA
    CACCCCTCCA ACTGGGCAGG GTTCATGCTT ATCGGCAGCG ATGTGAAGCT CAACAGTCCC
    TCTTCGCTCA TCAGTCAGGC CCTCACCGAG ATCCTCCAGC ACCCAGAGCG CGCGCGGGAC
    GCCCTGCGCG TGCTGCTGCA CCTGGTGGAG AAGTCGCTGC AGCGCATTCA GAACGGGCAG
    CGCAACGCCA TGTACACGTC CCAGCAGAGC GTGGAGAACA AAGTGGGCGG CATCCCTGGC
    TGGCAGGCCC TCCTCACCGC TGTGGGCTTC CGGCTGGACC CCCCGGCCAG CGGCCTGCCA
    GCAGCCGTCT TCTTCCCAAC CTCCGACCCG GGCGACCGGC TCCAGCAGTG CAGCAGCACC
    GTCCAGTCCC TGCTGGGTCT GCCCAACCCT GCCCTCCAAG CCCTCTGCAA GCTCATCACG
    GCCTCGGAGA CAGGCGAGCA GCTCATCAGC CGGCTCCACC AGGTGCTGGT GCAGCTGCAG
    GCCGGCGAGA AGGAGCAGGA CTTCGCCTCA GCGCCCATCC AGGTCTCCAT CAGCGTCCAG
    CTGTGGCGGC TCCCCGGCTG CCACGAGTTC CTGGCGGCTC TAGGTTTTGA TCTCTGTGAG
    GTCGGTCAGG AGGAAGTAAT CCTGAAAACC GGGAAACAAG CCAACCGACG GACCATGCAC
    TTTGCGCTCC AGGCCCTGCT CGCGCTCTTC GATTCCACGG AGCTGCCCAA GCGCCTCAGC
    CTGGACAGCT CGTCCTCCCT GGAGTCGCTG GCCTCGGCCC AGTCTGTCTC CAACGCTCTG
    CCCCTGGGCT TCCAGCACCC TCCTTTCTCC CCGACCGGGG CAGAGAGCAT AGCCTCAGAC
    GCCATCTCTG TGTACAGCCT GAGCTCCATT GCCTCCTCCA TGAGCTTCGT CTCCAAGCCT
    GACGGGGGGT CAGAAGGCGC GGTCCCTCGG GGACGGCAGG ACTACGAGCG GTCCAAGAAC
    ACCTACCCCC CTCGCGCTGC CCTGCCCCGG AGCCCGCTGT CCCCGCCTGC CCGCCCTGCA
    GGCGCCAAGG ACGAGGAGGA GTACGAGGGG TTCTCCATCA TTAGCACAGA GCCCTTGGCG
    GCCTCCCAGG AGGACCCACC GACGGGCTTC TCACCCGACC ACAAGCAGTC CCTCGTGGGG
    ACAGCGGGCG GCGTCAAAGT CTCGGTGAGC TCAAGCACTC CAAACTCTCC CGTTAAAATG
    ACTCTGATTC CCAGCCCAAA CTCACCCTTC CAGAAAGTTG GAAAACTAGC AAGTTCAGAT
    ACGGGAGAAT CAGACCAGTC GAGCACAGAA ACAGATAGTA CCGTGAAGTC CCAAGAAGAA
    AGTAACTCAA AGCTTGACCC TCAAGAGCTG GCACAGAAGA TTCTCGAGGA GACACAAAGC
    CATCTCATGG CAGTGGAGCG TCTTCAGAGG GGTGGCGGTC AGGGCGCCAG TTCTGAAGAT
    GGCATCTCTG TGCCCAACAG CGCTGCCGTC TTCCGAGCGT CCGAAACCAG CGCCTTCAGC
    AGGCCCGCCC TCTCCCACCA GAAGAGCCAG GCGGCGTTGT CGTCGTCGCC GGTCACCATC
    AAACCAAAGC CCCCAGCCAG GAGCTCCTCC CTCCCCAAGG TGAGCTCGGG GTACAGCAGC
    CCCACCGCCT CCGAGGCGTC CGCCAAAGAC AGCCCGAGCC AGCACAGCGG CCGGCCGTCG
    CCCGGCTCCG ACTCGCAGAC CTCCCTGAGC GACCAGCCTC TCTTCAAGCT GAAGTACCCC
    AGCTCCCCCT ACAGCGCTCA CATCTCCAAA TCTCCAAGGA ACATGTCCCC CAGCTCAGGC
    CACCAGTCTC CCGCGGGAAG CGCACCCTCC CCGGCTCTCT CCTACTCCTC GGCGGGCTCC
    GCGCGGTCCA GCCCCGCCGA CCACCCCGAC CTCGACAAGC TGAAGATGGC GGCCATCGAC
    GAGAAGGTGC AGGCCGTCCA CAACCTGAAG ATGTTCTGGC AGAGCACACC CCAGCACTCC
    ACGGGACCAA TGAAAATACT CCGGGGGGCC CCTGGAACCA TGACTTCCAA GAGAGATGTC
    CTCAGCCTAT TGAATTTGTC ACCTCGGCAC AACAAGAAGG AGGAGGGCGC AGACAAGCTG
    GAACTCAAGG AGCTGTCTTT GCAGAGACGT GGAGAAACCC CCCCCAAAGC CCCGCCCAAC
    GGACACTGGC GCACGGAGAC CACCTCGCTG AGCACGCTGC CGCTGCCCGC CCGCCCACCT
    GCCCCGGCTC CCACGCGCCC TCTCAGACTT CCTTCTGGAA ACGGCTACAA GTTTCTGTCC
    CCTGGAAGAT TTTTTCCTTC CTCTAAATGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224680
    Clone ID Related Accession (Same CDS sequence) XM_024977807.1
    Accession Version XM_024977807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGATGAAAT GGCAGCCCCG GAAGAAGGCA TACTTCCGAC AGGGTGTTGC CCTCCAGTAC 
    CTCGGACGTC ATGCCGATGC CCTGGCAGCC TTTGCGTCTG GGTTGGCTCA GGACCCCAAG
    AGTCTCCAGC TTCTGGTGGG GATGGTAGAA GCAGCCATGA AATCTCCCAT GAGAGACTCC
    CTCGAACCCA CTTATCAGCA GCTTCAGAAA ATGAAACTGG ACAAGAGCCC CTTTGTGGTT
    GTCTCTGTGG TTGGACAGGA GCTCCTGACA GCTGGCCATC ACGGGGCCTC TGTGGTTGTC
    TTAGAAGCCG CTCTGAAGAT TGGCACCTGC AGCCTCAAGC TGAGAGGTTC CGTTTTCTCT
    GCACTGAGCA GTGCTTACTG GTCTCTCGGG AACACAGAGA AGAGCACTGG ATACATGCAG
    CAGGACTTGG ATGTAGCCAA GACCTTAGGT GACCAGACAG GAGAATGCCG AGCTCATGGG
    AACCTGGGCT CTGCATTCTT CTCCAAAGGA AATTACCGGG AGGCTCTCAC TAACCACAGG
    CATCAGTTGG TGCTTGCCAT GAAACTCAAG GATCGAGAGG CAGCTTCATC AGCCCTGAGC
    AGTCTGGGCC ACGTGTACAC AGCCATTGGA GATTACCCCA ATGCACTGGC CAGTCACAAA
    CAGTGTGTTC TTCTCGCCAA GCAATCCAAA GATGAGCTTT CTGAAGCCCG AGAACTCGGC
    AACATGGGAG CTGTGTATAT TGCCATGGGT GACTTTGAGA ATGCTGTGCA GTGCCACGAG
    CAGCATCTGA AGATAGCCAA GGACCTGGGG AACAAGCGAG AAGAAGCCCG GGCCTACAGC
    AATCTGGGCA GTGCCTATCA CTACAGGAGG AACTTTGACA AGGCCATGTC TTACCACAAT
    TACGTGCTGG AGCTGGCGCA GGAGCTGATG GAGAAGGCCA TTGAGATGCG GGCCTACGCT
    GGCCTGGGCC ACGCTGCCAG GTGCATGCAG GATTTGGAGA GGGCCAAACA GTACCACGAG
    CAGCAGCTGG GCATTGCTGA GGATCTCAAG GACCGGGCTG CAGAGGGGCG AGCGTCTTCC
    AATCTGGGAA TCATACACCA GATGAAAGGT GATTATGACA CTGCACTGAA ACTCCACAAG
    ACCCACTTGT GCATCGCCCA GGAGCTGAGT GACTACGCGG CCCAGGGCCG CGCCTATGGA
    AACATGGGCA ACGCCTACAA TGCTCTGGGC ATGTACGACC AAGCGGTCAA GTACCACCGG
    CAGGAGCTAC AGATCTCCAT GGAAGTGAAC GACCGTGCCT CACAGGCATC CACGCACGGG
    AACCTCGCTG TGGCCTACCA GGCGCTGGGC GCCCACGACC GGGCCCTGCA GCACTATCAG
    AACCATTTGA ACATCGCCCG GGAGCTGCGA GACATCCAGA GCGAGGCGCG GGCTCTCAGC
    AACCTGGGCA ACTTCCACTG CTCTCGAGGG GAGTACGTCC AGGCCGCCCC CTATTACGAA
    CAGTACCTCC GGCTTGCTCC TGACCTTCAA GACATGGAGG GAGAAGGGAA GGTCTGCCAC
    AACCTCGGCT ATGCCCATTA CTGCCTTGGA AATTACCAGG AGGCGGTAAA GTACTATGAG
    CAGGATCTGG CCCTGGCCAA AGACCTTCAC GACAAATTGA GTCAAGCAAA AGCTTACTGC
    AACTTGGGCT TGGCATTCAA GGCTCTGCTG AATTTCAGCA AAGCTGAAGA GTGTCAAAAG
    TACCTACTGT CCCTAGCCCA GTCCCTGAAT AATTCCCAGG CTAAATTTAG AGCCCTGGGG
    AACCTGGGCG ATATATTCAT CTGTAAAAAA GATATCAATG GTGCAATAAA ATTCTATGAG
    CAGCAGCTGG GCTTGGCTCA CCACGTGAAG GACAGGAGAC TGGAGGCCAG TGCATATGCA
    GCCCTGGGCA CTGCATACCG AATGATCCAG AAACACGACA AGGCCTTGGG TTATCACACA
    CAGGAGCTGG AAGTATATCA GGAGCTGAGT GACCTGCCGG GAGAGTGCAG AGCTCATGGG
    CACCTGGCCG CAGTCTACAT GGCCCTGGGG AAGTACACAA TGGCTTTCAA GTGTTACGAA
    GAGCAGCTGG ATCTAGGGCA GAAGCTGAAG GACCCAAGTC TCGAAGCCCA GGTCTATGGC
    AACATGGGCA TCACAAAGAT GAACATGAAT GTGATGGAAG AAGCCATCGG CTACTTCGAG
    CAGCAGCTGG CCATGCTGCA GCAGCTGAGT GGGAACGAGT CTGTGCTTGA CAGGGGCCGG
    GCCTATGGCA ACCTGGGGGA CTGCTACGAG GCCCTGGGTG ACTACGAGGA AGCTATCAAG
    TACTATGAAC AGTATTTATC TGTTGCCCAG AGTCTGAATC GCATGCAAGA CCAAGCCAAG
    GCTTACCGAG GCCTGGGAAA TGGACACAGG GCAATGGGGA GCTTGCAGCA AGCCCTGGTG
    TGCTTTGAAA AGAGGCTTGT GGTCGCCCAT GAGCTCGGGG AGGCCTTTAA TAAAGCCCAG
    GCCTATGGCG AGCTGGGAAG TCTGCACAGC CAATTAGGAA ATTATGAACA AGCCATTTCC
    TGCCTTGAAC GCCAGCTGAA CATTGCTAGA GAGATGAAGG ACCGAGCTCT GGAAAGTGAC
    GCGGCCTGTG GCCTGGGGGG TGTGTACCAG CAGATGGGGG AGTACGATAC AGCCCTGCAG
    TACCACCAGT TGGATCTGCA GATTGCCGAG GAGACCAACA ACCCCACGTG CCAGGGCCGG
    GCCTACGGGA ACCTGGGCCT GACCTACGAG TCCCTGGGCA CCTTTGAGAG AGCCGTGGTC
    TATCAGGAAC AGCACTTGAG CATCGCTGCA CAGATGAACG ACTTGGTGGC CAAGACGGTG
    TCTTACAGCA GCCTTGGAAG GACGCATCAC GCTCTGCAGA ACTACTCCCA GGCAGTCATG
    TACTTGCAGG AAGGTTTAAG GTTAGCAGAG CAGCTGGGCC GAAGAGAAGA TGAAGCCAAG
    ATTCGCCATG GTCTTGGCCT CTCCCTCTGG GCGAGCGGGA ATCTGGAGGA GGCCCAACAT
    CAGCTCTACC GGGCATCAGC CTTGTTTGAG ACGATCCGAC ACGAGGCGCA GCTGAGCACA
    GACTACAAGC TCTCCCTCTT CGACCTGCAG ACGTCCTCCT ACCAGGCCTT GCAGCGAGTG
    CTCGTCAGCC TCGGCCATCA TGATGAAGCT CTGGCTGTGG CAGAAAGAGG ACGGACGAGG
    GCATTTGCAG ATCTCCTGGT GGAACGGCAA ACAGGACAGC AGGACTCTGA CCCCTACTCC
    CCGGTCACTA TCGATCAGAT CTTAGAGATG GTTAACGGCC AGAGGGGACT TGTGCTTTAC
    TACTCCCTGG CGGCGGGCTA CCTGTACAGC TGGCTGCTTG CTCCTGGGGC AGGAATTCTG
    AAGTTTCATG AGCACTACCT GGGAGACAGC ACAGTGGGAA GCCCCAGTGA CCCCCAGACG
    GGCGGCAGCG CGGCCCTTCC AACTGCCGCG AGCTCGGCCC TGGAGCAGCA CGTTGCCAGC
    GTGCGGGAGG CCCTGGGCGT GGAGTCCTAC TGCTCGAGGG CCTGTGCCAG CAGTGAGACC
    GAGAGTGAAG CTGGAGACAT CATGGACCAG CAGTTCGAGG AGATGAACAA CAGGCTCAAC
    TCGGTCACCG ACCCCACTGG CTTTCTTCGG ATGGTTCGCC GCAACAACCT CTTTAACAGG
    AGCTGCCAGA GCATGACCAG CCTGGTCGGC AACACCGCGT CGCCCATCAA GGACGGGACC
    TCCTCTCTTC CCAGGAGGCA GAGCTCATTC GCCAGGCCGC CGCTCCGCGC CCTGTACGAC
    CTGCTTGTGG CACCCATGGA AGGGGGCCTG ATGCACTCCA GCGGCCCAGT GGGCCGGCAC
    CGGCAGCTGG TCCTGGTCCT GGAGGGGGAC CTCTACCTCA TCCCGTTCGC CCTCCTGAAG
    GGAAGCTCCT CCAACGAGTA CCTGTACGAG CGCTTTGCCC TCATTGCCGT CCCCTCCATC
    CGCTCCCTCA GCCCGCAGGC CAAGTCTCAC CTGAGGAAGA GCCCGCCCGC CTATTCCAGC
    TCCACGTCCA TGGCGGCGGT GGTCGGCAAC CCCAAGCTGC CGTCGGCGGT GATGGACAGG
    TGGCTGTGGG GCCCGATGCC GTCAGCCGAG GAGGAAGCCT ACATGGTGTC CGAGCTGCTG
    GGCTGCCAGC CGCTGGTGGG CAGCGTGGCC ACCAAAGAGC GGGTCATGAG TGCCCTGACG
    CAGGCCGAGT GCGTCCACTT CGCCACCCAC ATCTCTTGGA AACTCTCGGC CTTGGTCCTC
    ACGCCCAGTG TGGACGGCGC CCCTGCAGGC GGCAAGAGCT CCTTCGGCCA CCCCTACACC
    ATCCCTGAGT CCCTGCGGGT GCAGGACGAC GCCAGCGACG GCGAGAGCAT CTCAGACTGC
    CCACCGCTGC AGGAGCTGCT GCTCACGGCC GCCGACATCC TGGACCTGCG GCTGCCCGTC
    AAGCTGGTGG TGCTCGGCTC CTCCCAGGAG TCCAGCAGCA GGATCACGGC CGATGGGGTG
    GTGGCGCTGA CTCGGGCCTT CCTGGCGGCG GGTGCTCAGT GTGTCCTCGT GTCTCTGTGG
    CCTGTGCCTG TGGCCGCTTC CAAGATGTTC CTGCACGCCC TGTACTCGTC CCTGCTGAAC
    GGCCTGAAGG CCAGTGCGGC ACTGGGCGAG GCCATGAAGG TGGTGCAGAG CAGCAAGGCC
    TTCTCACACC CCTCCAACTG GGCAGGGTTC ATGCTTATCG GCAGCGATGT GAAGCTCAAC
    AGTCCCTCTT CGCTCATCAG TCAGGCCCTC ACCGAGATCC TCCAGCACCC AGAGCGCGCG
    CGGGACGCCC TGCGCGTGCT GCTGCACCTG GTGGAGAAGT CGCTGCAGCG CATTCAGAAC
    GGGCAGCGCA ACGCCATGTA CACGTCCCAG CAGAGCGTGG AGAACAAAGT GGGCGGCATC
    CCTGGCTGGC AGGCCCTCCT CACCGCTGTG GGCTTCCGGC TGGACCCCCC GGCCAGCGGC
    CTGCCAGCAG CCGTCTTCTT CCCAACCTCC GACCCGGGCG ACCGGCTCCA GCAGTGCAGC
    AGCACCGTCC AGTCCCTGCT GGGTCTGCCC AACCCTGCCC TCCAAGCCCT CTGCAAGCTC
    ATCACGGCCT CGGAGACAGG CGAGCAGCTC ATCAGCCGGG CTGTTAAAAA TATGGTTGGA
    ATGCTCCACC AGGTGCTGGT GCAGCTGCAG GCCGGCGAGA AGGAGCAGGA CTTCGCCTCA
    GCGCCCATCC AGGTCTCCAT CAGCGTCCAG CTGTGGCGGC TCCCCGGCTG CCACGAGTTC
    CTGGCGGCTC TAGGTTTTGA TCTCTGTGAG GTCGGTCAGG AGGAAGTAAT CCTGAAAACC
    GGGAAACAAG CCAACCGACG GACCATGCAC TTTGCGCTCC AGGCCCTGCT CGCGCTCTTC
    GATTCCACGG AGCTGCCCAA GCGCCTCAGC CTGGACAGCT CGTCCTCCCT GGAGTCGCTG
    GCCTCGGCCC AGTCTGTCTC CAACGCTCTG CCCCTGGGCT TCCAGCACCC TCCTTTCTCC
    CCGACCGGGG CAGAGAGCAT AGCCTCAGAC GCCATCTCTG TGTACAGCCT GAGCTCCATT
    GCCTCCTCCA TGAGCTTCGT CTCCAAGCCT GACGGGGGGT CAGAAGGCGC GGTCCCTCGG
    GGACGGCAGG ACTACGAGCG GTCCAAGAAC ACCTACCCCC CTCGCGCTGC CCTGCCCCGG
    AGCCCGCTGT CCCCGCCTGC CCGCCCTGCA GGCGCCAAGG ACGAGGAGGA GTACGAGGGG
    TTCTCCATCA TTAGCACAGA GCCCTTGGCG GCCTCCCAGG AGGACCCACC GACGGGCTTC
    TCACCCGACC ACAAGCAGTC CCTCGTGGGG ACAGCGGGCG GCGTCAAAGT CTCGGTGAGC
    TCAAGCACTC CAAACTCTCC CGTTAAAATG ACTCTGATTC CCAGCCCAAA CTCACCCTTC
    CAGAAAGTTG GAAAACTAGC AAGTTCAGAT ACGGGAGAAT CAGACCAGTC GAGCACAGAA
    ACAGATAGTA CCGTGAAGTC CCAAGAAGAA AGTAACTCAA AGCTTGACCC TCAAGAGCTG
    GCACAGAAGA TTCTCGAGGA GACACAAAGC CATCTCATGG CAGTGGAGCG TCTTCAGAGG
    GGTGGCGGTC AGGGCGCCAG TTCTGAAGAT GGCATCTCTG TGCCCAACAG CGCTGCCGTC
    TTCCGAGCGT CCGAAACCAG CGCCTTCAGC AGGCCCGCCC TCTCCCACCA GAAGAGCCAG
    GCGGCGTTGT CGTCGTCGCC GGTCACCATC AAACCAAAGC CCCCAGCCAG GAGCTCCTCC
    CTCCCCAAGG TGAGCTCGGG GTACAGCAGC CCCACCGCCT CCGAGGCGTC CGCCAAAGAC
    AGCCCGAGCC AGCACAGCGG CCGGCCGTCG CCCGGCTCCG ACTCGCAGAC CTCCCTGAGC
    GACCAGCCTC TCTTCAAGCT GAAGTACCCC AGCTCCCCCT ACAGCGCTCA CATCTCCAAA
    TCTCCAAGGA ACATGTCCCC CAGCTCAGGC CACCAGTCTC CCGCGGGAAG CGCACCCTCC
    CCGGCTCTCT CCTACTCCTC GGCGGGCTCC GCGCGGTCCA GCCCCGCCGA CCACCCCGAC
    CTCGACAAGC TGAAGATGGC GGCCATCGAC GAGAAGGTGC AGGCCGTCCA CAACCTGAAG
    ATGTTCTGGC AGAGCACACC CCAGCACTCC ACGGGACCAA TGAAAATACT CCGGGGGGCC
    CCTGGAACCA TGACTTCCAA GAGAGATGTC CTCAGCCTAT TGAATTTGTC ACCTCGGCAC
    AACAAGAAGG AGGAGGGCGC AGACAAGCTG GAACTCAAGG AGCTGTCTTT GCAGAGACGT
    GGAGAAACCC CCCCCAAAGC CCCGCCCAAC GGACACTGGC GCACGGAGAC CACCTCGCTG
    AGCACGCTGC CGCTGCCCGC CCGCCCACCT GCCCCGGCTC CCACGCGCCC TCTCAGACTT
    CCTTCTGGAA ACGGCTACAA GTTTCTGTCC CCTGGAAGAT TTTTTCCTTC CTCTAAATGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833575.1
    CDS6955..14037
    Translation

    Target ORF information:

    RefSeq Version XM_024977807.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGATGAAAT GGCAGCCCCG GAAGAAGGCA TACTTCCGAC AGGGTGTTGC CCTCCAGTAC 
    CTCGGACGTC ATGCCGATGC CCTGGCAGCC TTTGCGTCTG GGTTGGCTCA GGACCCCAAG
    AGTCTCCAGC TTCTGGTGGG GATGGTAGAA GCAGCCATGA AATCTCCCAT GAGAGACTCC
    CTCGAACCCA CTTATCAGCA GCTTCAGAAA ATGAAACTGG ACAAGAGCCC CTTTGTGGTT
    GTCTCTGTGG TTGGACAGGA GCTCCTGACA GCTGGCCATC ACGGGGCCTC TGTGGTTGTC
    TTAGAAGCCG CTCTGAAGAT TGGCACCTGC AGCCTCAAGC TGAGAGGTTC CGTTTTCTCT
    GCACTGAGCA GTGCTTACTG GTCTCTCGGG AACACAGAGA AGAGCACTGG ATACATGCAG
    CAGGACTTGG ATGTAGCCAA GACCTTAGGT GACCAGACAG GAGAATGCCG AGCTCATGGG
    AACCTGGGCT CTGCATTCTT CTCCAAAGGA AATTACCGGG AGGCTCTCAC TAACCACAGG
    CATCAGTTGG TGCTTGCCAT GAAACTCAAG GATCGAGAGG CAGCTTCATC AGCCCTGAGC
    AGTCTGGGCC ACGTGTACAC AGCCATTGGA GATTACCCCA ATGCACTGGC CAGTCACAAA
    CAGTGTGTTC TTCTCGCCAA GCAATCCAAA GATGAGCTTT CTGAAGCCCG AGAACTCGGC
    AACATGGGAG CTGTGTATAT TGCCATGGGT GACTTTGAGA ATGCTGTGCA GTGCCACGAG
    CAGCATCTGA AGATAGCCAA GGACCTGGGG AACAAGCGAG AAGAAGCCCG GGCCTACAGC
    AATCTGGGCA GTGCCTATCA CTACAGGAGG AACTTTGACA AGGCCATGTC TTACCACAAT
    TACGTGCTGG AGCTGGCGCA GGAGCTGATG GAGAAGGCCA TTGAGATGCG GGCCTACGCT
    GGCCTGGGCC ACGCTGCCAG GTGCATGCAG GATTTGGAGA GGGCCAAACA GTACCACGAG
    CAGCAGCTGG GCATTGCTGA GGATCTCAAG GACCGGGCTG CAGAGGGGCG AGCGTCTTCC
    AATCTGGGAA TCATACACCA GATGAAAGGT GATTATGACA CTGCACTGAA ACTCCACAAG
    ACCCACTTGT GCATCGCCCA GGAGCTGAGT GACTACGCGG CCCAGGGCCG CGCCTATGGA
    AACATGGGCA ACGCCTACAA TGCTCTGGGC ATGTACGACC AAGCGGTCAA GTACCACCGG
    CAGGAGCTAC AGATCTCCAT GGAAGTGAAC GACCGTGCCT CACAGGCATC CACGCACGGG
    AACCTCGCTG TGGCCTACCA GGCGCTGGGC GCCCACGACC GGGCCCTGCA GCACTATCAG
    AACCATTTGA ACATCGCCCG GGAGCTGCGA GACATCCAGA GCGAGGCGCG GGCTCTCAGC
    AACCTGGGCA ACTTCCACTG CTCTCGAGGG GAGTACGTCC AGGCCGCCCC CTATTACGAA
    CAGTACCTCC GGCTTGCTCC TGACCTTCAA GACATGGAGG GAGAAGGGAA GGTCTGCCAC
    AACCTCGGCT ATGCCCATTA CTGCCTTGGA AATTACCAGG AGGCGGTAAA GTACTATGAG
    CAGGATCTGG CCCTGGCCAA AGACCTTCAC GACAAATTGA GTCAAGCAAA AGCTTACTGC
    AACTTGGGCT TGGCATTCAA GGCTCTGCTG AATTTCAGCA AAGCTGAAGA GTGTCAAAAG
    TACCTACTGT CCCTAGCCCA GTCCCTGAAT AATTCCCAGG CTAAATTTAG AGCCCTGGGG
    AACCTGGGCG ATATATTCAT CTGTAAAAAA GATATCAATG GTGCAATAAA ATTCTATGAG
    CAGCAGCTGG GCTTGGCTCA CCACGTGAAG GACAGGAGAC TGGAGGCCAG TGCATATGCA
    GCCCTGGGCA CTGCATACCG AATGATCCAG AAACACGACA AGGCCTTGGG TTATCACACA
    CAGGAGCTGG AAGTATATCA GGAGCTGAGT GACCTGCCGG GAGAGTGCAG AGCTCATGGG
    CACCTGGCCG CAGTCTACAT GGCCCTGGGG AAGTACACAA TGGCTTTCAA GTGTTACGAA
    GAGCAGCTGG ATCTAGGGCA GAAGCTGAAG GACCCAAGTC TCGAAGCCCA GGTCTATGGC
    AACATGGGCA TCACAAAGAT GAACATGAAT GTGATGGAAG AAGCCATCGG CTACTTCGAG
    CAGCAGCTGG CCATGCTGCA GCAGCTGAGT GGGAACGAGT CTGTGCTTGA CAGGGGCCGG
    GCCTATGGCA ACCTGGGGGA CTGCTACGAG GCCCTGGGTG ACTACGAGGA AGCTATCAAG
    TACTATGAAC AGTATTTATC TGTTGCCCAG AGTCTGAATC GCATGCAAGA CCAAGCCAAG
    GCTTACCGAG GCCTGGGAAA TGGACACAGG GCAATGGGGA GCTTGCAGCA AGCCCTGGTG
    TGCTTTGAAA AGAGGCTTGT GGTCGCCCAT GAGCTCGGGG AGGCCTTTAA TAAAGCCCAG
    GCCTATGGCG AGCTGGGAAG TCTGCACAGC CAATTAGGAA ATTATGAACA AGCCATTTCC
    TGCCTTGAAC GCCAGCTGAA CATTGCTAGA GAGATGAAGG ACCGAGCTCT GGAAAGTGAC
    GCGGCCTGTG GCCTGGGGGG TGTGTACCAG CAGATGGGGG AGTACGATAC AGCCCTGCAG
    TACCACCAGT TGGATCTGCA GATTGCCGAG GAGACCAACA ACCCCACGTG CCAGGGCCGG
    GCCTACGGGA ACCTGGGCCT GACCTACGAG TCCCTGGGCA CCTTTGAGAG AGCCGTGGTC
    TATCAGGAAC AGCACTTGAG CATCGCTGCA CAGATGAACG ACTTGGTGGC CAAGACGGTG
    TCTTACAGCA GCCTTGGAAG GACGCATCAC GCTCTGCAGA ACTACTCCCA GGCAGTCATG
    TACTTGCAGG AAGGTTTAAG GTTAGCAGAG CAGCTGGGCC GAAGAGAAGA TGAAGCCAAG
    ATTCGCCATG GTCTTGGCCT CTCCCTCTGG GCGAGCGGGA ATCTGGAGGA GGCCCAACAT
    CAGCTCTACC GGGCATCAGC CTTGTTTGAG ACGATCCGAC ACGAGGCGCA GCTGAGCACA
    GACTACAAGC TCTCCCTCTT CGACCTGCAG ACGTCCTCCT ACCAGGCCTT GCAGCGAGTG
    CTCGTCAGCC TCGGCCATCA TGATGAAGCT CTGGCTGTGG CAGAAAGAGG ACGGACGAGG
    GCATTTGCAG ATCTCCTGGT GGAACGGCAA ACAGGACAGC AGGACTCTGA CCCCTACTCC
    CCGGTCACTA TCGATCAGAT CTTAGAGATG GTTAACGGCC AGAGGGGACT TGTGCTTTAC
    TACTCCCTGG CGGCGGGCTA CCTGTACAGC TGGCTGCTTG CTCCTGGGGC AGGAATTCTG
    AAGTTTCATG AGCACTACCT GGGAGACAGC ACAGTGGGAA GCCCCAGTGA CCCCCAGACG
    GGCGGCAGCG CGGCCCTTCC AACTGCCGCG AGCTCGGCCC TGGAGCAGCA CGTTGCCAGC
    GTGCGGGAGG CCCTGGGCGT GGAGTCCTAC TGCTCGAGGG CCTGTGCCAG CAGTGAGACC
    GAGAGTGAAG CTGGAGACAT CATGGACCAG CAGTTCGAGG AGATGAACAA CAGGCTCAAC
    TCGGTCACCG ACCCCACTGG CTTTCTTCGG ATGGTTCGCC GCAACAACCT CTTTAACAGG
    AGCTGCCAGA GCATGACCAG CCTGGTCGGC AACACCGCGT CGCCCATCAA GGACGGGACC
    TCCTCTCTTC CCAGGAGGCA GAGCTCATTC GCCAGGCCGC CGCTCCGCGC CCTGTACGAC
    CTGCTTGTGG CACCCATGGA AGGGGGCCTG ATGCACTCCA GCGGCCCAGT GGGCCGGCAC
    CGGCAGCTGG TCCTGGTCCT GGAGGGGGAC CTCTACCTCA TCCCGTTCGC CCTCCTGAAG
    GGAAGCTCCT CCAACGAGTA CCTGTACGAG CGCTTTGCCC TCATTGCCGT CCCCTCCATC
    CGCTCCCTCA GCCCGCAGGC CAAGTCTCAC CTGAGGAAGA GCCCGCCCGC CTATTCCAGC
    TCCACGTCCA TGGCGGCGGT GGTCGGCAAC CCCAAGCTGC CGTCGGCGGT GATGGACAGG
    TGGCTGTGGG GCCCGATGCC GTCAGCCGAG GAGGAAGCCT ACATGGTGTC CGAGCTGCTG
    GGCTGCCAGC CGCTGGTGGG CAGCGTGGCC ACCAAAGAGC GGGTCATGAG TGCCCTGACG
    CAGGCCGAGT GCGTCCACTT CGCCACCCAC ATCTCTTGGA AACTCTCGGC CTTGGTCCTC
    ACGCCCAGTG TGGACGGCGC CCCTGCAGGC GGCAAGAGCT CCTTCGGCCA CCCCTACACC
    ATCCCTGAGT CCCTGCGGGT GCAGGACGAC GCCAGCGACG GCGAGAGCAT CTCAGACTGC
    CCACCGCTGC AGGAGCTGCT GCTCACGGCC GCCGACATCC TGGACCTGCG GCTGCCCGTC
    AAGCTGGTGG TGCTCGGCTC CTCCCAGGAG TCCAGCAGCA GGATCACGGC CGATGGGGTG
    GTGGCGCTGA CTCGGGCCTT CCTGGCGGCG GGTGCTCAGT GTGTCCTCGT GTCTCTGTGG
    CCTGTGCCTG TGGCCGCTTC CAAGATGTTC CTGCACGCCC TGTACTCGTC CCTGCTGAAC
    GGCCTGAAGG CCAGTGCGGC ACTGGGCGAG GCCATGAAGG TGGTGCAGAG CAGCAAGGCC
    TTCTCACACC CCTCCAACTG GGCAGGGTTC ATGCTTATCG GCAGCGATGT GAAGCTCAAC
    AGTCCCTCTT CGCTCATCAG TCAGGCCCTC ACCGAGATCC TCCAGCACCC AGAGCGCGCG
    CGGGACGCCC TGCGCGTGCT GCTGCACCTG GTGGAGAAGT CGCTGCAGCG CATTCAGAAC
    GGGCAGCGCA ACGCCATGTA CACGTCCCAG CAGAGCGTGG AGAACAAAGT GGGCGGCATC
    CCTGGCTGGC AGGCCCTCCT CACCGCTGTG GGCTTCCGGC TGGACCCCCC GGCCAGCGGC
    CTGCCAGCAG CCGTCTTCTT CCCAACCTCC GACCCGGGCG ACCGGCTCCA GCAGTGCAGC
    AGCACCGTCC AGTCCCTGCT GGGTCTGCCC AACCCTGCCC TCCAAGCCCT CTGCAAGCTC
    ATCACGGCCT CGGAGACAGG CGAGCAGCTC ATCAGCCGGG CTGTTAAAAA TATGGTTGGA
    ATGCTCCACC AGGTGCTGGT GCAGCTGCAG GCCGGCGAGA AGGAGCAGGA CTTCGCCTCA
    GCGCCCATCC AGGTCTCCAT CAGCGTCCAG CTGTGGCGGC TCCCCGGCTG CCACGAGTTC
    CTGGCGGCTC TAGGTTTTGA TCTCTGTGAG GTCGGTCAGG AGGAAGTAAT CCTGAAAACC
    GGGAAACAAG CCAACCGACG GACCATGCAC TTTGCGCTCC AGGCCCTGCT CGCGCTCTTC
    GATTCCACGG AGCTGCCCAA GCGCCTCAGC CTGGACAGCT CGTCCTCCCT GGAGTCGCTG
    GCCTCGGCCC AGTCTGTCTC CAACGCTCTG CCCCTGGGCT TCCAGCACCC TCCTTTCTCC
    CCGACCGGGG CAGAGAGCAT AGCCTCAGAC GCCATCTCTG TGTACAGCCT GAGCTCCATT
    GCCTCCTCCA TGAGCTTCGT CTCCAAGCCT GACGGGGGGT CAGAAGGCGC GGTCCCTCGG
    GGACGGCAGG ACTACGAGCG GTCCAAGAAC ACCTACCCCC CTCGCGCTGC CCTGCCCCGG
    AGCCCGCTGT CCCCGCCTGC CCGCCCTGCA GGCGCCAAGG ACGAGGAGGA GTACGAGGGG
    TTCTCCATCA TTAGCACAGA GCCCTTGGCG GCCTCCCAGG AGGACCCACC GACGGGCTTC
    TCACCCGACC ACAAGCAGTC CCTCGTGGGG ACAGCGGGCG GCGTCAAAGT CTCGGTGAGC
    TCAAGCACTC CAAACTCTCC CGTTAAAATG ACTCTGATTC CCAGCCCAAA CTCACCCTTC
    CAGAAAGTTG GAAAACTAGC AAGTTCAGAT ACGGGAGAAT CAGACCAGTC GAGCACAGAA
    ACAGATAGTA CCGTGAAGTC CCAAGAAGAA AGTAACTCAA AGCTTGACCC TCAAGAGCTG
    GCACAGAAGA TTCTCGAGGA GACACAAAGC CATCTCATGG CAGTGGAGCG TCTTCAGAGG
    GGTGGCGGTC AGGGCGCCAG TTCTGAAGAT GGCATCTCTG TGCCCAACAG CGCTGCCGTC
    TTCCGAGCGT CCGAAACCAG CGCCTTCAGC AGGCCCGCCC TCTCCCACCA GAAGAGCCAG
    GCGGCGTTGT CGTCGTCGCC GGTCACCATC AAACCAAAGC CCCCAGCCAG GAGCTCCTCC
    CTCCCCAAGG TGAGCTCGGG GTACAGCAGC CCCACCGCCT CCGAGGCGTC CGCCAAAGAC
    AGCCCGAGCC AGCACAGCGG CCGGCCGTCG CCCGGCTCCG ACTCGCAGAC CTCCCTGAGC
    GACCAGCCTC TCTTCAAGCT GAAGTACCCC AGCTCCCCCT ACAGCGCTCA CATCTCCAAA
    TCTCCAAGGA ACATGTCCCC CAGCTCAGGC CACCAGTCTC CCGCGGGAAG CGCACCCTCC
    CCGGCTCTCT CCTACTCCTC GGCGGGCTCC GCGCGGTCCA GCCCCGCCGA CCACCCCGAC
    CTCGACAAGC TGAAGATGGC GGCCATCGAC GAGAAGGTGC AGGCCGTCCA CAACCTGAAG
    ATGTTCTGGC AGAGCACACC CCAGCACTCC ACGGGACCAA TGAAAATACT CCGGGGGGCC
    CCTGGAACCA TGACTTCCAA GAGAGATGTC CTCAGCCTAT TGAATTTGTC ACCTCGGCAC
    AACAAGAAGG AGGAGGGCGC AGACAAGCTG GAACTCAAGG AGCTGTCTTT GCAGAGACGT
    GGAGAAACCC CCCCCAAAGC CCCGCCCAAC GGACACTGGC GCACGGAGAC CACCTCGCTG
    AGCACGCTGC CGCTGCCCGC CCGCCCACCT GCCCCGGCTC CCACGCGCCC TCTCAGACTT
    CCTTCTGGAA ACGGCTACAA GTTTCTGTCC CCTGGAAGAT TTTTTCCTTC CTCTAAATGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224681
    Clone ID Related Accession (Same CDS sequence) XM_024977808.1
    Accession Version XM_024977808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGATGAAAT GGCAGCCCCG GAAGAAGGCA TACTTCCGAC AGGGTGTTGC CCTCCAGTAC 
    CTCGGACGTC ATGCCGATGC CCTGGCAGCC TTTGCGTCTG GGTTGGCTCA GGACCCCAAG
    AGTCTCCAGC TTCTGGTGGG GATGGTAGAA GCAGCCATGA AATCTCCCAT GAGAGACTCC
    CTCGAACCCA CTTATCAGCA GCTTCAGAAA ATGAAACTGG ACAAGAGCCC CTTTGTGGTT
    GTCTCTGTGG TTGGACAGGA GCTCCTGACA GCTGGCCATC ACGGGGCCTC TGTGGTTGTC
    TTAGAAGCCG CTCTGAAGAT TGGCACCTGC AGCCTCAAGC TGAGAGGTTC CGTTTTCTCT
    GCACTGAGCA GTGCTTACTG GTCTCTCGGG AACACAGAGA AGAGCACTGG ATACATGCAG
    CAGGACTTGG ATGTAGCCAA GACCTTAGGT GACCAGACAG GAGAATGCCG AGCTCATGGG
    AACCTGGGCT CTGCATTCTT CTCCAAAGGA AATTACCGGG AGGCTCTCAC TAACCACAGG
    CATCAGTTGG TGCTTGCCAT GAAACTCAAG GATCGAGAGG CAGCTTCATC AGCCCTGAGC
    AGTCTGGGCC ACGTGTACAC AGCCATTGGA GATTACCCCA ATGCACTGGC CAGTCACAAA
    CAGTGTGTTC TTCTCGCCAA GCAATCCAAA GATGAGCTTT CTGAAGCCCG AGAACTCGGC
    AACATGGGAG CTGTGTATAT TGCCATGGGT GACTTTGAGA ATGCTGTGCA GTGCCACGAG
    CAGCATCTGA AGATAGCCAA GGACCTGGGG AACAAGCGAG AAGAAGCCCG GGCCTACAGC
    AATCTGGGCA GTGCCTATCA CTACAGGAGG AACTTTGACA AGGCCATGTC TTACCACAAT
    TACGTGCTGG AGCTGGCGCA GGAGCTGATG GAGAAGGCCA TTGAGATGCG GGCCTACGCT
    GGCCTGGGCC ACGCTGCCAG GTGCATGCAG GATTTGGAGA GGGCCAAACA GTACCACGAG
    CAGCAGCTGG GCATTGCTGA GGATCTCAAG GACCGGGCTG CAGAGGGGCG AGCGTCTTCC
    AATCTGGGAA TCATACACCA GATGAAAGGT GATTATGACA CTGCACTGAA ACTCCACAAG
    ACCCACTTGT GCATCGCCCA GGAGCTGAGT GACTACGCGG CCCAGGGCCG CGCCTATGGA
    AACATGGGCA ACGCCTACAA TGCTCTGGGC ATGTACGACC AAGCGGTCAA GTACCACCGG
    CAGGAGCTAC AGATCTCCAT GGAAGTGAAC GACCGTGCCT CACAGGCATC CACGCACGGG
    AACCTCGCTG TGGCCTACCA GGCGCTGGGC GCCCACGACC GGGCCCTGCA GCACTATCAG
    AACCATTTGA ACATCGCCCG GGAGCTGCGA GACATCCAGA GCGAGGCGCG GGCTCTCAGC
    AACCTGGGCA ACTTCCACTG CTCTCGAGGG GAGTACGTCC AGGCCGCCCC CTATTACGAA
    CAGTACCTCC GGCTTGCTCC TGACCTTCAA GACATGGAGG GAGAAGGGAA GGTCTGCCAC
    AACCTCGGCT ATGCCCATTA CTGCCTTGGA AATTACCAGG AGGCGGTAAA GTACTATGAG
    CAGGATCTGG CCCTGGCCAA AGACCTTCAC GACAAATTGA GTCAAGCAAA AGCTTACTGC
    AACTTGGGCT TGGCATTCAA GGCTCTGCTG AATTTCAGCA AAGCTGAAGA GTGTCAAAAG
    TACCTACTGT CCCTAGCCCA GTCCCTGAAT AATTCCCAGG CTAAATTTAG AGCCCTGGGG
    AACCTGGGCG ATATATTCAT CTGTAAAAAA GATATCAATG GTGCAATAAA ATTCTATGAG
    CAGCAGCTGG GCTTGGCTCA CCACGTGAAG GACAGGAGAC TGGAGGCCAG TGCATATGCA
    GCCCTGGGCA CTGCATACCG AATGATCCAG AAACACGACA AGGCCTTGGG TTATCACACA
    CAGGAGCTGG AAGTATATCA GGAGCTGAGT GACCTGCCGG GAGAGTGCAG AGCTCATGGG
    CACCTGGCCG CAGTCTACAT GGCCCTGGGG AAGTACACAA TGGCTTTCAA GTGTTACGAA
    GAGCAGCTGG ATCTAGGGCA GAAGCTGAAG GACCCAAGTC TCGAAGCCCA GGTCTATGGC
    AACATGGGCA TCACAAAGAT GAACATGAAT GTGATGGAAG AAGCCATCGG CTACTTCGAG
    CAGCAGCTGG CCATGCTGCA GCAGCTGAGT GGGAACGAGT CTGTGCTTGA CAGGGGCCGG
    GCCTATGGCA ACCTGGGGGA CTGCTACGAG GCCCTGGGTG ACTACGAGGA AGCTATCAAG
    TACTATGAAC AGTATTTATC TGTTGCCCAG AGTCTGAATC GCATGCAAGA CCAAGCCAAG
    GCTTACCGAG GCCTGGGAAA TGGACACAGG GCAATGGGGA GCTTGCAGCA AGCCCTGGTG
    TGCTTTGAAA AGAGGCTTGT GGTCGCCCAT GAGCTCGGGG AGGCCTTTAA TAAAGCCCAG
    GCCTATGGCG AGCTGGGAAG TCTGCACAGC CAATTAGGAA ATTATGAACA AGCCATTTCC
    TGCCTTGAAC GCCAGCTGAA CATTGCTAGA GAGATGAAGG ACCGAGCTCT GGAAAGTGAC
    GCGGCCTGTG GCCTGGGGGG TGTGTACCAG CAGATGGGGG AGTACGATAC AGCCCTGCAG
    TACCACCAGT TGGATCTGCA GATTGCCGAG GAGACCAACA ACCCCACGTG CCAGGGCCGG
    GCCTACGGGA ACCTGGGCCT GACCTACGAG TCCCTGGGCA CCTTTGAGAG AGCCGTGGTC
    TATCAGGAAC AGCACTTGAG CATCGCTGCA CAGATGAACG ACTTGGTGGC CAAGACGGTG
    TCTTACAGCA GCCTTGGAAG GACGCATCAC GCTCTGCAGA ACTACTCCCA GGCAGTCATG
    TACTTGCAGG AAGGTTTAAG GTTAGCAGAG CAGCTGGGCC GAAGAGAAGA TGAAGCCAAG
    ATTCGCCATG GTCTTGGCCT CTCCCTCTGG GCGAGCGGGA ATCTGGAGGA GGCCCAACAT
    CAGCTCTACC GGGCATCAGC CTTGTTTGAG ACGATCCGAC ACGAGGCGCA GCTGAGCACA
    GACTACAAGC TCTCCCTCTT CGACCTGCAG ACGTCCTCCT ACCAGGCCTT GCAGCGAGTG
    CTCGTCAGCC TCGGCCATCA TGATGAAGCT CTGGCTGTGG CAGAAAGAGG ACGGACGAGG
    GCATTTGCAG ATCTCCTGGT GGAACGGCAA ACAGGACAGC AGGACTCTGA CCCCTACTCC
    CCGGTCACTA TCGATCAGAT CTTAGAGATG GTTAACGGCC AGAGGGGACT TGTGCTTTAC
    TACTCCCTGG CGGCGGGCTA CCTGTACAGC TGGCTGCTTG CTCCTGGGGC AGGAATTCTG
    AAGTTTCATG AGCACTACCT GGGAGACAGC ACAGTGGGAA GCCCCAGTGA CCCCCAGACG
    GGCGGCAGCG CGGCCCTTCC AACTGCCGCG AGCTCGGCCC TGGAGCAGCA CGTTGCCAGC
    GTGCGGGAGG CCCTGGGCGT GGAGTCCTAC TGCTCGAGGG CCTGTGCCAG CAGTGAGACC
    GAGAGTGAAG CTGGAGACAT CATGGACCAG CAGTTCGAGG AGATGAACAA CAGGCTCAAC
    TCGGTCACCG ACCCCACTGG CTTTCTTCGG ATGGTTCGCC GCAACAACCT CTTTAACAGG
    AGCTGCCAGA GCATGACCAG CCTGGTCGGC AACACCGCGT CGCCCATCAA GGACGGGACC
    TCCTCTCTTC CCAGGAGGCA GAGCTCATTC GCCAGGCCGC CGCTCCGCGC CCTGTACGAC
    CTGCTTGTGG CACCCATGGA AGGGGGCCTG ATGCACTCCA GCGGCCCAGT GGGCCGGCAC
    CGGCAGCTGG TCCTGGTCCT GGAGGGGGAC CTCTACCTCA TCCCGTTCGC CCTCCTGAAG
    GGAAGCTCCT CCAACGAGTA CCTGTACGAG CGCTTTGCCC TCATTGCCGT CCCCTCCATC
    CGCTCCCTCA GCCCGCAGGC CAAGTCTCAC CTGAGGAAGA GCCCGCCCGC CTATTCCAGC
    TCCACGTCCA TGGCGGCGGT GGTCGGCAAC CCCAAGCTGC CGTCGGCGGT GATGGACAGG
    TGGCTGTGGG GCCCGATGCC GTCAGCCGAG GAGGAAGCCT ACATGGTGTC CGAGCTGCTG
    GGCTGCCAGC CGCTGGTGGG CAGCGTGGCC ACCAAAGAGC GGGTCATGAG TGCCCTGACG
    CAGGCCGAGT GCGTCCACTT CGCCACCCAC ATCTCTTGGA AACTCTCGGC CTTGGTCCTC
    ACGCCCAGTG TGGACGGCGC CCCTGCAGGC GGCAAGAGCT CCTTCGGCCA CCCCTACACC
    ATCCCTGAGT CCCTGCGGGT GCAGGACGAC GCCAGCGACG GCGAGAGCAT CTCAGACTGC
    CCACCGCTGC AGGAGCTGCT GCTCACGGCC GCCGACATCC TGGACCTGCG GCTGCCCGTC
    AAGCTGGTGG TGCTCGGCTC CTCCCAGGAG TCCAGCAGCA GGATCACGGC CGATGGGGTG
    GTGGCGCTGA CTCGGGCCTT CCTGGCGGCG GGTGCTCAGT GTGTCCTCGT GTCTCTGTGG
    CCTGTGCCTG TGGCCGCTTC CAAGATGTTC CTGCACGCCC TGTACTCGTC CCTGCTGAAC
    GGCCTGAAGG CCAGTGCGGC ACTGGGCGAG GCCATGAAGG TGGTGCAGAG CAGCAAGGCC
    TTCTCACACC CCTCCAACTG GGCAGGGTTC ATGCTTATCG GCAGCGATGT GAAGCTCAAC
    AGTCCCTCTT CGCTCATCAG TCAGGCCCTC ACCGAGATCC TCCAGCACCC AGAGCGCGCG
    CGGGACGCCC TGCGCGTGCT GCTGCACCTG GTGGAGAAGT CGCTGCAGCG CATTCAGAAC
    GGGCAGCGCA ACGCCATGTA CACGTCCCAG CAGAGCGTGG AGAACAAAGT GGGCGGCATC
    CCTGGCTGGC AGGCCCTCCT CACCGCTGTG GGCTTCCGGC TGGACCCCCC GGCCAGCGGC
    CTGCCAGCAG CCGTCTTCTT CCCAACCTCC GACCCGGGCG ACCGGCTCCA GCAGTGCAGC
    AGCACCGTCC AGTCCCTGCT GGGTCTGCCC AACCCTGCCC TCCAAGCCCT CTGCAAGCTC
    ATCACGGCCT CGGAGACAGG CGAGCAGCTC ATCAGCCGGC TCCACCAGGT GCTGGTGCAG
    CTGCAGGCCG GCGAGAAGGA GCAGGACTTC GCCTCAGCGC CCATCCAGGT CTCCATCAGC
    GTCCAGCTGT GGCGGCTCCC CGGCTGCCAC GAGTTCCTGG CGGCTCTAGG TTTTGATCTC
    TGTGAGGTCG GTCAGGAGGA AGTAATCCTG AAAACCGGGA AACAAGCCAA CCGACGGACC
    ATGCACTTTG CGCTCCAGGC CCTGCTCGCG CTCTTCGATT CCACGGAGCT GCCCAAGCGC
    CTCAGCCTGG ACAGCTCGTC CTCCCTGGAG TCGCTGGCCT CGGCCCAGTC TGTCTCCAAC
    GCTCTGCCCC TGGGCTTCCA GCACCCTCCT TTCTCCCCGA CCGGGGCAGA GAGCATAGCC
    TCAGACGCCA TCTCTGTGTA CAGCCTGAGC TCCATTGCCT CCTCCATGAG CTTCGTCTCC
    AAGCCTGACG GGGGGTCAGA AGGCGCGGTC CCTCGGGGAC GGCAGGACTA CGAGCGGTCC
    AAGAACACCT ACCCCCCTCG CGCTGCCCTG CCCCGGAGCC CGCTGTCCCC GCCTGCCCGC
    CCTGCAGGCG CCAAGGACGA GGAGGAGTAC GAGGGGTTCT CCATCATTAG CACAGAGCCC
    TTGGCGGCCT CCCAGGAGGA CCCACCGACG GGCTTCTCAC CCGACCACAA GCAGTCCCTC
    GTGGGGACAG CGGGCGGCGT CAAAGTCTCG GTGAGCTCAA GCACTCCAAA CTCTCCCGTT
    AAAATGACTC TGATTCCCAG CCCAAACTCA CCCTTCCAGA AAGTTGGAAA ACTAGCAAGT
    TCAGATACGG GAGAATCAGA CCAGTCGAGC ACAGAAACAG ATAGTACCGT GAAGTCCCAA
    GAAGAAAGTA ACTCAAAGCT TGACCCTCAA GAGCTGGCAC AGAAGATTCT CGAGGAGACA
    CAAAGCCATC TCATGGCAGT GGAGCGTCTT CAGAGGGGTG GCGGTCAGGG CGCCAGTTCT
    GAAGATGGCA TCTCTGTGCC CAACAGCGCT GCCGTCTTCC GAGCGTCCGA AACCAGCGCC
    TTCAGCAGGC CCGCCCTCTC CCACCAGAAG AGCCAGGCGG CGTTGTCGTC GTCGCCGGTC
    ACCATCAAAC CAAAGCCCCC AGCCAGGAGC TCCTCCCTCC CCAAGGTGAG CTCGGGGTAC
    AGCAGCCCCA CCGCCTCCGA GGCGTCCGCC AAAGACAGCC CGAGCCAGCA CAGCGGCCGG
    CCGTCGCCCG GCTCCGACTC GCAGACCTCC CTGAGCGACC AGCCTCTCTT CAAGCTGAAG
    TACCCCAGCT CCCCCTACAG CGCTCACATC TCCAAATCTC CAAGGAACAT GTCCCCCAGC
    TCAGGCCACC AGTCTCCCGC GGGAAGCGCA CCCTCCCCGG CTCTCTCCTA CTCCTCGGCG
    GGCTCCGCGC GGTCCAGCCC CGCCGACCAC CCCGACCTCG ACAAGCTGAA GATGGCGGCC
    ATCGACGAGA AGGTGCAGGC CGTCCACAAC CTGAAGATGT TCTGGCAGAG CACACCCCAG
    CACTCCACGG GACCAATGAA AATACTCCGG GGGGCCCCTG GAACCATGAC TTCCAAGAGA
    GATGTCCTCA GCCTATTGAA TTTGTCACCT CGGCACAACA AGAAGGAGGA GGGCGCAGAC
    AAGCTGGAAC TCAAGGAGCT GTCTTTGCAG AGACGTGGAG AAACCCCCCC CAAAGCCCCG
    CCCAACGGAC ACTGGCGCAC GGAGACCACC TCGCTGAGCA CGCTGCCGCT GCCCGCCCGC
    CCACCTGCCC CGGCTCCCAC GCGCCCTCTC AGACTTCCTT CTGGAAACGG CTACAAGTTT
    CTGTCCCCTG GAAGATTTTT TCCTTCCTCT AAATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833576.1
    CDS6954..14012
    Translation

    Target ORF information:

    RefSeq Version XM_024977808.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGATGAAAT GGCAGCCCCG GAAGAAGGCA TACTTCCGAC AGGGTGTTGC CCTCCAGTAC 
    CTCGGACGTC ATGCCGATGC CCTGGCAGCC TTTGCGTCTG GGTTGGCTCA GGACCCCAAG
    AGTCTCCAGC TTCTGGTGGG GATGGTAGAA GCAGCCATGA AATCTCCCAT GAGAGACTCC
    CTCGAACCCA CTTATCAGCA GCTTCAGAAA ATGAAACTGG ACAAGAGCCC CTTTGTGGTT
    GTCTCTGTGG TTGGACAGGA GCTCCTGACA GCTGGCCATC ACGGGGCCTC TGTGGTTGTC
    TTAGAAGCCG CTCTGAAGAT TGGCACCTGC AGCCTCAAGC TGAGAGGTTC CGTTTTCTCT
    GCACTGAGCA GTGCTTACTG GTCTCTCGGG AACACAGAGA AGAGCACTGG ATACATGCAG
    CAGGACTTGG ATGTAGCCAA GACCTTAGGT GACCAGACAG GAGAATGCCG AGCTCATGGG
    AACCTGGGCT CTGCATTCTT CTCCAAAGGA AATTACCGGG AGGCTCTCAC TAACCACAGG
    CATCAGTTGG TGCTTGCCAT GAAACTCAAG GATCGAGAGG CAGCTTCATC AGCCCTGAGC
    AGTCTGGGCC ACGTGTACAC AGCCATTGGA GATTACCCCA ATGCACTGGC CAGTCACAAA
    CAGTGTGTTC TTCTCGCCAA GCAATCCAAA GATGAGCTTT CTGAAGCCCG AGAACTCGGC
    AACATGGGAG CTGTGTATAT TGCCATGGGT GACTTTGAGA ATGCTGTGCA GTGCCACGAG
    CAGCATCTGA AGATAGCCAA GGACCTGGGG AACAAGCGAG AAGAAGCCCG GGCCTACAGC
    AATCTGGGCA GTGCCTATCA CTACAGGAGG AACTTTGACA AGGCCATGTC TTACCACAAT
    TACGTGCTGG AGCTGGCGCA GGAGCTGATG GAGAAGGCCA TTGAGATGCG GGCCTACGCT
    GGCCTGGGCC ACGCTGCCAG GTGCATGCAG GATTTGGAGA GGGCCAAACA GTACCACGAG
    CAGCAGCTGG GCATTGCTGA GGATCTCAAG GACCGGGCTG CAGAGGGGCG AGCGTCTTCC
    AATCTGGGAA TCATACACCA GATGAAAGGT GATTATGACA CTGCACTGAA ACTCCACAAG
    ACCCACTTGT GCATCGCCCA GGAGCTGAGT GACTACGCGG CCCAGGGCCG CGCCTATGGA
    AACATGGGCA ACGCCTACAA TGCTCTGGGC ATGTACGACC AAGCGGTCAA GTACCACCGG
    CAGGAGCTAC AGATCTCCAT GGAAGTGAAC GACCGTGCCT CACAGGCATC CACGCACGGG
    AACCTCGCTG TGGCCTACCA GGCGCTGGGC GCCCACGACC GGGCCCTGCA GCACTATCAG
    AACCATTTGA ACATCGCCCG GGAGCTGCGA GACATCCAGA GCGAGGCGCG GGCTCTCAGC
    AACCTGGGCA ACTTCCACTG CTCTCGAGGG GAGTACGTCC AGGCCGCCCC CTATTACGAA
    CAGTACCTCC GGCTTGCTCC TGACCTTCAA GACATGGAGG GAGAAGGGAA GGTCTGCCAC
    AACCTCGGCT ATGCCCATTA CTGCCTTGGA AATTACCAGG AGGCGGTAAA GTACTATGAG
    CAGGATCTGG CCCTGGCCAA AGACCTTCAC GACAAATTGA GTCAAGCAAA AGCTTACTGC
    AACTTGGGCT TGGCATTCAA GGCTCTGCTG AATTTCAGCA AAGCTGAAGA GTGTCAAAAG
    TACCTACTGT CCCTAGCCCA GTCCCTGAAT AATTCCCAGG CTAAATTTAG AGCCCTGGGG
    AACCTGGGCG ATATATTCAT CTGTAAAAAA GATATCAATG GTGCAATAAA ATTCTATGAG
    CAGCAGCTGG GCTTGGCTCA CCACGTGAAG GACAGGAGAC TGGAGGCCAG TGCATATGCA
    GCCCTGGGCA CTGCATACCG AATGATCCAG AAACACGACA AGGCCTTGGG TTATCACACA
    CAGGAGCTGG AAGTATATCA GGAGCTGAGT GACCTGCCGG GAGAGTGCAG AGCTCATGGG
    CACCTGGCCG CAGTCTACAT GGCCCTGGGG AAGTACACAA TGGCTTTCAA GTGTTACGAA
    GAGCAGCTGG ATCTAGGGCA GAAGCTGAAG GACCCAAGTC TCGAAGCCCA GGTCTATGGC
    AACATGGGCA TCACAAAGAT GAACATGAAT GTGATGGAAG AAGCCATCGG CTACTTCGAG
    CAGCAGCTGG CCATGCTGCA GCAGCTGAGT GGGAACGAGT CTGTGCTTGA CAGGGGCCGG
    GCCTATGGCA ACCTGGGGGA CTGCTACGAG GCCCTGGGTG ACTACGAGGA AGCTATCAAG
    TACTATGAAC AGTATTTATC TGTTGCCCAG AGTCTGAATC GCATGCAAGA CCAAGCCAAG
    GCTTACCGAG GCCTGGGAAA TGGACACAGG GCAATGGGGA GCTTGCAGCA AGCCCTGGTG
    TGCTTTGAAA AGAGGCTTGT GGTCGCCCAT GAGCTCGGGG AGGCCTTTAA TAAAGCCCAG
    GCCTATGGCG AGCTGGGAAG TCTGCACAGC CAATTAGGAA ATTATGAACA AGCCATTTCC
    TGCCTTGAAC GCCAGCTGAA CATTGCTAGA GAGATGAAGG ACCGAGCTCT GGAAAGTGAC
    GCGGCCTGTG GCCTGGGGGG TGTGTACCAG CAGATGGGGG AGTACGATAC AGCCCTGCAG
    TACCACCAGT TGGATCTGCA GATTGCCGAG GAGACCAACA ACCCCACGTG CCAGGGCCGG
    GCCTACGGGA ACCTGGGCCT GACCTACGAG TCCCTGGGCA CCTTTGAGAG AGCCGTGGTC
    TATCAGGAAC AGCACTTGAG CATCGCTGCA CAGATGAACG ACTTGGTGGC CAAGACGGTG
    TCTTACAGCA GCCTTGGAAG GACGCATCAC GCTCTGCAGA ACTACTCCCA GGCAGTCATG
    TACTTGCAGG AAGGTTTAAG GTTAGCAGAG CAGCTGGGCC GAAGAGAAGA TGAAGCCAAG
    ATTCGCCATG GTCTTGGCCT CTCCCTCTGG GCGAGCGGGA ATCTGGAGGA GGCCCAACAT
    CAGCTCTACC GGGCATCAGC CTTGTTTGAG ACGATCCGAC ACGAGGCGCA GCTGAGCACA
    GACTACAAGC TCTCCCTCTT CGACCTGCAG ACGTCCTCCT ACCAGGCCTT GCAGCGAGTG
    CTCGTCAGCC TCGGCCATCA TGATGAAGCT CTGGCTGTGG CAGAAAGAGG ACGGACGAGG
    GCATTTGCAG ATCTCCTGGT GGAACGGCAA ACAGGACAGC AGGACTCTGA CCCCTACTCC
    CCGGTCACTA TCGATCAGAT CTTAGAGATG GTTAACGGCC AGAGGGGACT TGTGCTTTAC
    TACTCCCTGG CGGCGGGCTA CCTGTACAGC TGGCTGCTTG CTCCTGGGGC AGGAATTCTG
    AAGTTTCATG AGCACTACCT GGGAGACAGC ACAGTGGGAA GCCCCAGTGA CCCCCAGACG
    GGCGGCAGCG CGGCCCTTCC AACTGCCGCG AGCTCGGCCC TGGAGCAGCA CGTTGCCAGC
    GTGCGGGAGG CCCTGGGCGT GGAGTCCTAC TGCTCGAGGG CCTGTGCCAG CAGTGAGACC
    GAGAGTGAAG CTGGAGACAT CATGGACCAG CAGTTCGAGG AGATGAACAA CAGGCTCAAC
    TCGGTCACCG ACCCCACTGG CTTTCTTCGG ATGGTTCGCC GCAACAACCT CTTTAACAGG
    AGCTGCCAGA GCATGACCAG CCTGGTCGGC AACACCGCGT CGCCCATCAA GGACGGGACC
    TCCTCTCTTC CCAGGAGGCA GAGCTCATTC GCCAGGCCGC CGCTCCGCGC CCTGTACGAC
    CTGCTTGTGG CACCCATGGA AGGGGGCCTG ATGCACTCCA GCGGCCCAGT GGGCCGGCAC
    CGGCAGCTGG TCCTGGTCCT GGAGGGGGAC CTCTACCTCA TCCCGTTCGC CCTCCTGAAG
    GGAAGCTCCT CCAACGAGTA CCTGTACGAG CGCTTTGCCC TCATTGCCGT CCCCTCCATC
    CGCTCCCTCA GCCCGCAGGC CAAGTCTCAC CTGAGGAAGA GCCCGCCCGC CTATTCCAGC
    TCCACGTCCA TGGCGGCGGT GGTCGGCAAC CCCAAGCTGC CGTCGGCGGT GATGGACAGG
    TGGCTGTGGG GCCCGATGCC GTCAGCCGAG GAGGAAGCCT ACATGGTGTC CGAGCTGCTG
    GGCTGCCAGC CGCTGGTGGG CAGCGTGGCC ACCAAAGAGC GGGTCATGAG TGCCCTGACG
    CAGGCCGAGT GCGTCCACTT CGCCACCCAC ATCTCTTGGA AACTCTCGGC CTTGGTCCTC
    ACGCCCAGTG TGGACGGCGC CCCTGCAGGC GGCAAGAGCT CCTTCGGCCA CCCCTACACC
    ATCCCTGAGT CCCTGCGGGT GCAGGACGAC GCCAGCGACG GCGAGAGCAT CTCAGACTGC
    CCACCGCTGC AGGAGCTGCT GCTCACGGCC GCCGACATCC TGGACCTGCG GCTGCCCGTC
    AAGCTGGTGG TGCTCGGCTC CTCCCAGGAG TCCAGCAGCA GGATCACGGC CGATGGGGTG
    GTGGCGCTGA CTCGGGCCTT CCTGGCGGCG GGTGCTCAGT GTGTCCTCGT GTCTCTGTGG
    CCTGTGCCTG TGGCCGCTTC CAAGATGTTC CTGCACGCCC TGTACTCGTC CCTGCTGAAC
    GGCCTGAAGG CCAGTGCGGC ACTGGGCGAG GCCATGAAGG TGGTGCAGAG CAGCAAGGCC
    TTCTCACACC CCTCCAACTG GGCAGGGTTC ATGCTTATCG GCAGCGATGT GAAGCTCAAC
    AGTCCCTCTT CGCTCATCAG TCAGGCCCTC ACCGAGATCC TCCAGCACCC AGAGCGCGCG
    CGGGACGCCC TGCGCGTGCT GCTGCACCTG GTGGAGAAGT CGCTGCAGCG CATTCAGAAC
    GGGCAGCGCA ACGCCATGTA CACGTCCCAG CAGAGCGTGG AGAACAAAGT GGGCGGCATC
    CCTGGCTGGC AGGCCCTCCT CACCGCTGTG GGCTTCCGGC TGGACCCCCC GGCCAGCGGC
    CTGCCAGCAG CCGTCTTCTT CCCAACCTCC GACCCGGGCG ACCGGCTCCA GCAGTGCAGC
    AGCACCGTCC AGTCCCTGCT GGGTCTGCCC AACCCTGCCC TCCAAGCCCT CTGCAAGCTC
    ATCACGGCCT CGGAGACAGG CGAGCAGCTC ATCAGCCGGC TCCACCAGGT GCTGGTGCAG
    CTGCAGGCCG GCGAGAAGGA GCAGGACTTC GCCTCAGCGC CCATCCAGGT CTCCATCAGC
    GTCCAGCTGT GGCGGCTCCC CGGCTGCCAC GAGTTCCTGG CGGCTCTAGG TTTTGATCTC
    TGTGAGGTCG GTCAGGAGGA AGTAATCCTG AAAACCGGGA AACAAGCCAA CCGACGGACC
    ATGCACTTTG CGCTCCAGGC CCTGCTCGCG CTCTTCGATT CCACGGAGCT GCCCAAGCGC
    CTCAGCCTGG ACAGCTCGTC CTCCCTGGAG TCGCTGGCCT CGGCCCAGTC TGTCTCCAAC
    GCTCTGCCCC TGGGCTTCCA GCACCCTCCT TTCTCCCCGA CCGGGGCAGA GAGCATAGCC
    TCAGACGCCA TCTCTGTGTA CAGCCTGAGC TCCATTGCCT CCTCCATGAG CTTCGTCTCC
    AAGCCTGACG GGGGGTCAGA AGGCGCGGTC CCTCGGGGAC GGCAGGACTA CGAGCGGTCC
    AAGAACACCT ACCCCCCTCG CGCTGCCCTG CCCCGGAGCC CGCTGTCCCC GCCTGCCCGC
    CCTGCAGGCG CCAAGGACGA GGAGGAGTAC GAGGGGTTCT CCATCATTAG CACAGAGCCC
    TTGGCGGCCT CCCAGGAGGA CCCACCGACG GGCTTCTCAC CCGACCACAA GCAGTCCCTC
    GTGGGGACAG CGGGCGGCGT CAAAGTCTCG GTGAGCTCAA GCACTCCAAA CTCTCCCGTT
    AAAATGACTC TGATTCCCAG CCCAAACTCA CCCTTCCAGA AAGTTGGAAA ACTAGCAAGT
    TCAGATACGG GAGAATCAGA CCAGTCGAGC ACAGAAACAG ATAGTACCGT GAAGTCCCAA
    GAAGAAAGTA ACTCAAAGCT TGACCCTCAA GAGCTGGCAC AGAAGATTCT CGAGGAGACA
    CAAAGCCATC TCATGGCAGT GGAGCGTCTT CAGAGGGGTG GCGGTCAGGG CGCCAGTTCT
    GAAGATGGCA TCTCTGTGCC CAACAGCGCT GCCGTCTTCC GAGCGTCCGA AACCAGCGCC
    TTCAGCAGGC CCGCCCTCTC CCACCAGAAG AGCCAGGCGG CGTTGTCGTC GTCGCCGGTC
    ACCATCAAAC CAAAGCCCCC AGCCAGGAGC TCCTCCCTCC CCAAGGTGAG CTCGGGGTAC
    AGCAGCCCCA CCGCCTCCGA GGCGTCCGCC AAAGACAGCC CGAGCCAGCA CAGCGGCCGG
    CCGTCGCCCG GCTCCGACTC GCAGACCTCC CTGAGCGACC AGCCTCTCTT CAAGCTGAAG
    TACCCCAGCT CCCCCTACAG CGCTCACATC TCCAAATCTC CAAGGAACAT GTCCCCCAGC
    TCAGGCCACC AGTCTCCCGC GGGAAGCGCA CCCTCCCCGG CTCTCTCCTA CTCCTCGGCG
    GGCTCCGCGC GGTCCAGCCC CGCCGACCAC CCCGACCTCG ACAAGCTGAA GATGGCGGCC
    ATCGACGAGA AGGTGCAGGC CGTCCACAAC CTGAAGATGT TCTGGCAGAG CACACCCCAG
    CACTCCACGG GACCAATGAA AATACTCCGG GGGGCCCCTG GAACCATGAC TTCCAAGAGA
    GATGTCCTCA GCCTATTGAA TTTGTCACCT CGGCACAACA AGAAGGAGGA GGGCGCAGAC
    AAGCTGGAAC TCAAGGAGCT GTCTTTGCAG AGACGTGGAG AAACCCCCCC CAAAGCCCCG
    CCCAACGGAC ACTGGCGCAC GGAGACCACC TCGCTGAGCA CGCTGCCGCT GCCCGCCCGC
    CCACCTGCCC CGGCTCCCAC GCGCCCTCTC AGACTTCCTT CTGGAAACGG CTACAAGTTT
    CTGTCCCCTG GAAGATTTTT TCCTTCCTCT AAATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93256
    Clone ID Related Accession (Same CDS sequence) XM_617836.9 , XM_015475572.1
    Accession Version XM_617836.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_617836.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGAAGAATA TTCCTCTCTT TGGTGCTAAG ACTATTGGCC GGAGAAGTCC TGATGGACCA 
    GTGCTGAGCA AAGCTGAATT TGTTGAGAAA GTTCGTCAGA GTAATCAGGC CTGTCATGAT
    GGAGATTTCC ACACAGCTAT TGTTCTCTAC AATGAAGCCC TGGCTGTTGA CCCTCAGAAC
    TGTATCTTAT ACAGCAATAG ATCTGCAGCC TATATGAAAA TCCAGCAGTA TGACAAGGCA
    CTGGATGATG CAATCAAAGC TCGACTTCTC AATCCCAAGT GGCCAAAGGC ATACTTCCGA
    CAGGGTGTTG CCCTCCAGTA CCTCGGACGT CATGCCGATG CCCTGGCAGC CTTTGCGTCT
    GGGTTGGCTC AGGACCCCAA GAGTCTCCAG CTTCTGGTGG GGATGGTAGA AGCAGCCATG
    AAATCTCCCA TGAGAGACTC CCTCGAACCC ACTTATCAGC AGCTTCAGAA AATGAAACTG
    GACAAGAGCC CCTTTGTGGT TGTCTCTGTG GTTGGACAGG AGCTCCTGAC AGCTGGCCAT
    CACGGGGCCT CTGTGGTTGT CTTAGAAGCC GCTCTGAAGA TTGGCACCTG CAGCCTCAAG
    CTGAGAGGTT CCGTTTTCTC TGCACTGAGC AGTGCTTACT GGTCTCTCGG GAACACAGAG
    AAGAGCACTG GATACATGCA GCAGGACTTG GATGTAGCCA AGACCTTAGG TGACCAGACA
    GGAGAATGCC GAGCTCATGG GAACCTGGGC TCTGCATTCT TCTCCAAAGG AAATTACCGG
    GAGGCTCTCA CTAACCACAG GCATCAGTTG GTGCTTGCCA TGAAACTCAA GGATCGAGAG
    GCAGCTTCAT CAGCCCTGAG CAGTCTGGGC CACGTGTACA CAGCCATTGG AGATTACCCC
    AATGCACTGG CCAGTCACAA ACAGTGTGTT CTTCTCGCCA AGCAATCCAA AGATGAGCTT
    TCTGAAGCCC GAGAACTCGG CAACATGGGA GCTGTGTATA TTGCCATGGG TGACTTTGAG
    AATGCTGTGC AGTGCCACGA GCAGCATCTG AAGATAGCCA AGGACCTGGG GAACAAGCGA
    GAAGAAGCCC GGGCCTACAG CAATCTGGGC AGTGCCTATC ACTACAGGAG GAACTTTGAC
    AAGGCCATGT CTTACCACAA TTACGTGCTG GAGCTGGCGC AGGAGCTGAT GGAGAAGGCC
    ATTGAGATGC GGGCCTACGC TGGCCTGGGC CACGCTGCCA GGTGCATGCA GGATTTGGAG
    AGGGCCAAAC AGTACCACGA GCAGCAGCTG GGCATTGCTG AGGATCTCAA GGACCGGGCT
    GCAGAGGGGC GAGCGTCTTC CAATCTGGGA ATCATACACC AGATGAAAGG TGATTATGAC
    ACTGCACTGA AACTCCACAA GACCCACTTG TGCATCGCCC AGGAGCTGAG TGACTACGCG
    GCCCAGGGCC GCGCCTATGG AAACATGGGC AACGCCTACA ATGCTCTGGG CATGTACGAC
    CAAGCGGTCA AGTACCACCG GCAGGAGCTA CAGATCTCCA TGGAAGTGAA CGACCGTGCC
    TCACAGGCAT CCACGCACGG GAACCTCGCT GTGGCCTACC AGGCGCTGGG CGCCCACGAC
    CGGGCCCTGC AGCACTATCA GAACCATTTG AACATCGCCC GGGAGCTGCG AGACATCCAG
    AGCGAGGCGC GGGCTCTCAG CAACCTGGGC AACTTCCACT GCTCTCGAGG GGAGTACGTC
    CAGGCCGCCC CCTATTACGA ACAGTACCTC CGGCTTGCTC CTGACCTTCA AGACATGGAG
    GGAGAAGGGA AGGTCTGCCA CAACCTCGGC TATGCCCATT ACTGCCTTGG AAATTACCAG
    GAGGCGGTAA AGTACTATGA GCAGGATCTG GCCCTGGCCA AAGACCTTCA CGACAAATTG
    AGTCAAGCAA AAGCTTACTG CAACTTGGGC TTGGCATTCA AGGCTCTGCT GAATTTCAGC
    AAAGCTGAAG AGTGTCAAAA GTACCTACTG TCCCTAGCCC AGTCCCTGAA TAATTCCCAG
    GCTAAATTTA GAGCCCTGGG GAACCTGGGC GATATATTCA TCTGTAAAAA AGATATCAAT
    GGTGCAATAA AATTCTATGA GCAGCAGCTG GGCTTGGCTC ACCACGTGAA GGACAGGAGA
    CTGGAGGCCA GTGCATATGC AGCCCTGGGC ACTGCATACC GAATGATCCA GAAACACGAC
    AAGGCCTTGG GTTATCACAC ACAGGAGCTG GAAGTATATC AGGAGCTGAG TGACCTGCCG
    GGAGAGTGCA GAGCTCATGG GCACCTGGCC GCAGTCTACA TGGCCCTGGG GAAGTACACA
    ATGGCTTTCA AGTGTTACGA AGAGCAGCTG GATCTAGGGC AGAAGCTGAA GGACCCAAGT
    CTCGAAGCCC AGGTCTATGG CAACATGGGC ATCACAAAGA TGAACATGAA TGTGATGGAA
    GAAGCCATCG GCTACTTCGA GCAGCAGCTG GCCATGCTGC AGCAGCTGAG TGGGAACGAG
    TCTGTGCTTG ACAGGGGCCG GGCCTATGGC AACCTGGGGG ACTGCTACGA GGCCCTGGGT
    GACTACGAGG AAGCTATCAA GTACTATGAA CAGTATTTAT CTGTTGCCCA GAGTCTGAAT
    CGCATGCAAG ACCAAGCCAA GGCTTACCGA GGCCTGGGAA ATGGACACAG GGCAATGGGG
    AGCTTGCAGC AAGCCCTGGT GTGCTTTGAA AAGAGGCTTG TGGTCGCCCA TGAGCTCGGG
    GAGGCCTTTA ATAAAGCCCA GGCCTATGGC GAGCTGGGAA GTCTGCACAG CCAATTAGGA
    AATTATGAAC AAGCCATTTC CTGCCTTGAA CGCCAGCTGA ACATTGCTAG AGAGATGAAG
    GACCGAGCTC TGGAAAGTGA CGCGGCCTGT GGCCTGGGGG GTGTGTACCA GCAGATGGGG
    GAGTACGATA CAGCCCTGCA GTACCACCAG TTGGATCTGC AGATTGCCGA GGAGACCAAC
    AACCCCACGT GCCAGGGCCG GGCCTACGGG AACCTGGGCC TGACCTACGA GTCCCTGGGC
    ACCTTTGAGA GAGCCGTGGT CTATCAGGAA CAGCACTTGA GCATCGCTGC ACAGATGAAC
    GACTTGGTGG CCAAGACGGT GTCTTACAGC AGCCTTGGAA GGACGCATCA CGCTCTGCAG
    AACTACTCCC AGGCAGTCAT GTACTTGCAG GAAGGTTTAA GGTTAGCAGA GCAGCTGGGC
    CGAAGAGAAG ATGAAGCCAA GATTCGCCAT GGTCTTGGCC TCTCCCTCTG GGCGAGCGGG
    AATCTGGAGG AGGCCCAACA TCAGCTCTAC CGGGCATCAG CCTTGTTTGA GACGATCCGA
    CACGAGGCGC AGCTGAGCAC AGACTACAAG CTCTCCCTCT TCGACCTGCA GACGTCCTCC
    TACCAGGCCT TGCAGCGAGT GCTCGTCAGC CTCGGCCATC ATGATGAAGC TCTGGCTGTG
    GCAGAAAGAG GACGGACGAG GGCATTTGCA GATCTCCTGG TGGAACGGCA AACAGGACAG
    CAGGACTCTG ACCCCTACTC CCCGGTCACT ATCGATCAGA TCTTAGAGAT GGTTAACGGC
    CAGAGGGGAC TTGTGCTTTA CTACTCCCTG GCGGCGGGCT ACCTGTACAG CTGGCTGCTT
    GCTCCTGGGG CAGGAATTCT GAAGTTTCAT GAGCACTACC TGGGAGACAG CACAGTGGGA
    AGCCCCAGTG ACCCCCAGAC GGGCGGCAGC GCGGCCCTTC CAACTGCCGC GAGCTCGGCC
    CTGGAGCAGC ACGTTGCCAG CGTGCGGGAG GCCCTGGGCG TGGAGTCCTA CTGCTCGAGG
    GCCTGTGCCA GCAGTGAGAC CGAGAGTGAA GCTGGAGACA TCATGGACCA GCAGTTCGAG
    GAGATGAACA ACAGGCTCAA CTCGGTCACC GACCCCACTG GCTTTCTTCG GATGGTTCGC
    CGCAACAACC TCTTTAACAG GAGCTGCCAG AGCATGACCA GCCTGGTCGG CAACACCGCG
    TCGCCCATCA AGGACGGGAC CTCCTCTCTT CCCAGGAGGC AGAGCTCATT CGCCAGGCCG
    CCGCTCCGCG CCCTGTACGA CCTGCTTGTG GCACCCATGG AAGGGGGCCT GATGCACTCC
    AGCGGCCCAG TGGGCCGGCA CCGGCAGCTG GTCCTGGTCC TGGAGGGGGA CCTCTACCTC
    ATCCCGTTCG CCCTCCTGAA GGGAAGCTCC TCCAACGAGT ACCTGTACGA GCGCTTTGCC
    CTCATTGCCG TCCCCTCCAT CCGCTCCCTC AGCCCGCAGG CCAAGTCTCA CCTGAGGAAG
    AGCCCGCCCG CCTATTCCAG CTCCACGTCC ATGGCGGCGG TGGTCGGCAA CCCCAAGCTG
    CCGTCGGCGG TGATGGACAG GTGGCTGTGG GGCCCGATGC CGTCAGCCGA GGAGGAAGCC
    TACATGGTGT CCGAGCTGCT GGGCTGCCAG CCGCTGGTGG GCAGCGTGGC CACCAAAGAG
    CGGGTCATGA GTGCCCTGAC GCAGGCCGAG TGCGTCCACT TCGCCACCCA CATCTCTTGG
    AAACTCTCGG CCTTGGTCCT CACGCCCAGT GTGGACGGCG CCCCTGCAGG CGGCAAGAGC
    TCCTTCGGCC ACCCCTACAC CATCCCTGAG TCCCTGCGGG TGCAGGACGA CGCCAGCGAC
    GGCGAGAGCA TCTCAGACTG CCCACCGCTG CAGGAGCTGC TGCTCACGGC CGCCGACATC
    CTGGACCTGC GGCTGCCCGT CAAGCTGGTG GTGCTCGGCT CCTCCCAGGA GTCCAGCAGC
    AGGATCACGG CCGATGGGGT GGTGGCGCTG ACTCGGGCCT TCCTGGCGGC GGGTGCTCAG
    TGTGTCCTCG TGTCTCTGTG GCCTGTGCCT GTGGCCGCTT CCAAGATGTT CCTGCACGCC
    CTGTACTCGT CCCTGCTGAA CGGCCTGAAG GCCAGTGCGG CACTGGGCGA GGCCATGAAG
    GTGGTGCAGA GCAGCAAGGC CTTCTCACAC CCCTCCAACT GGGCAGGGTT CATGCTTATC
    GGCAGCGATG TGAAGCTCAA CAGTCCCTCT TCGCTCATCG GTCAGGCCCT CACCGAGATC
    CTCCAGCACC CGGAGCGCGC GCGGGACGCC CTGCGCGTGC TGCTGCACCT GGTGGAGAAG
    TCGCTGCAGC GCATTCAGAA CGGGCAGCGC AACGCCATGT ACACGTCCCA GCAGAGCGTG
    GAGAACAAAG TGGGCGGCAT CCCTGGCTGG CAGGCCCTCC TCACCGCTGT GGGCTTCCGG
    CTGGACCCCC CGGCCAGCGG CCTGCCAGCA GCCGTCTTCT TCCCAACCTC CGACCCGGGC
    GACCGGCTCC AGCAGTGCAG CAGCACCGTC CAGTCCCTGC TGGGTCTGCC CAACCCTGCC
    CTCCAAGCCC TCTGCAAGCT CATCACGGCC TCGGAGACAG GCGAGCAGCT CATCAGCCGG
    GCTGTTAAAA ATATGGTTGG AATGCTCCAC CAGGTGCTGG TGCAGCTGCA GGCCGGCGAG
    AAGGAGCAGG ACTTCGCCTC AGCGCCCATC CAGGTCTCCA TCAGCGTCCA GCTGTGGCGG
    CTCCCCGGCT GCCACGAGTT CCTGGCGGCT CTAGGTTTTG ATCTCTGTGA GGTCGGTCAG
    GAGGAAGTAA TCCTGAAAAC CGGGAAACAA GCCAACCGAC GGACCATGCA CTTTGCGCTC
    CAGGCCCTGC TCGCGCTCTT CGATTCCACG GAGCTGCCCA AGCGCCTCAG CCTGGACAGC
    TCGTCCTCCC TGGAGTCGCT GGCCTCGGCC CAGTCTGTCT CCAACGCTCT GCCCCTGGGC
    TTCCAGCACC CTCCTTTCTC CCCGACCGGG GCAGAGAGCA TAGCCTCAGA CGCCATCTCT
    GTGTACAGCC TGAGCTCCAT TGCCTCCTCC ATGAGCTTCG TCTCCAAGCC TGACGGGGGG
    TCAGAAGGCG CGGTCCCTCG GGGACGGCAG GACTACGAGC GGTCCAAGAA CACCTACCCC
    CCTCGCGCTG CCCTGCCCCG GAGCCCGCTG TCCCCGCCTG CCCGCCCTGC AGGCGCCAAG
    GACGAGGAGG AGTACGAGGG GTTCTCCATC ATTAGCACAG AGCCCTTGGC GGCCTCCCAG
    GAGGACCCAC CGACGGGCTT CTCACCCGAC CACAAGCAGT CCCTCGTGGG GACAGCGGGC
    GGCGTCAAAG TCTCGGTGAG CTCAAGCACT CCAAACTCTC CCGTTAAAAT GACTCTGATT
    CCCAGCCCAA ACTCACCCTT CCAGAAAGTT GGAAAACTAG CAAGTTCAGA TACGGGAGAA
    TCAGACCAGT CGAGCACAGA AACAGATAGT ACCGTGAAGT CCCAAGAAGA AAGTAACTCA
    AAGCTTGACC CTCAAGAGCT GGCACAGAAG ATTCTCGAGG AGACACAAAG CCATCTCATG
    GCAGTGGAGC GTCTTCAGAG GGGTGGCGGT CAGGGCGCCA GTTCTGAAGA TGGCATCTCT
    GTGCCCAACA GCGCTGCCGT CTTCCGAGCG TCCGAAACCA GCGCCTTCAG CAGGCCCGCC
    CTCTCCCACC AGAAGAGCCA GGCGGCGTCG TCGTCGTCGC CGGTCACCAT CAAACCAAAG
    CCCCCAGCCA GGAGCTCCTC CCTCCCCAAG GTGAGCTCGG GGTACAGCAG CCCCACCGCC
    TCCGAGGCGT CCGCCAAAGA CAGCCCGAGC CAGCACAGCG GCCGGCCGTC GCCCGGCTCC
    GACTCGCAGA CCTCCCTGAG CGACCAGCCT CTCTTCAAGC TGAAGTACCC CAGCTCCCCC
    TACAGCGCTC ACATCTCCAA ATCTCCAAGG AACATGTCCC CCAGCTCAGG CCACCAGTCT
    CCCGCGGGAA GCGCACCCTC CCCGGCTCTC TCCTACTCCT CGGCGGGCTC CGCGCGGTCC
    AGCCCCGCCG ACCACCCCGA CCTCGACAAG CTGAAGATGG CGGCCATCGA CGAGAAGGTG
    CAGGCCGTCC ACAACCTGAA GATGTTCTGG CAGAGCACAC CCCAGCACTC CACGGGACCA
    ATGAAAATAC TCCGGGGGGC CCCTGGAACC ATGACTTCCA AGAGAGATGT CCTCAGCCTA
    TTGAATTTGT CACCTCGGCA CAACAAGAAG GAGGAGGGCG CAGACAAGCT GGAACTCAAG
    GAGCTGTCTT TGCAGAGACG TGGAGAAACC CCCCCCAAAG CCCCGCCCAA CGGACACTGG
    CGCACGGAGA CCACCTCGCT GAGCACGCTG CCGCTGCCCG CCCGCCCACC TGCCCCGGCT
    CCCACGCGCC CTCTCAGACT TCCTTCTGGA AACGGCTACA AGTTTCTGTC CCCTGGAAGA
    TTTTTTCCTT CCTCTAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_617836.7
    CDS1..7344
    Translation

    Target ORF information:

    RefSeq Version XM_617836.9
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_617836.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGAAGAATA TTCCTCTCTT TGGTGCTAAG ACTATTGGCC GGAGAAGTCC TGATGGACCA 
    GTGCTGAGCA AAGCTGAATT TGTTGAGAAA GTTCGTCAGA GTAATCAGGC CTGTCATGAT
    GGAGATTTCC ACACAGCTAT TGTTCTCTAC AATGAAGCCC TGGCTGTTGA CCCTCAGAAC
    TGTATCTTAT ACAGCAATAG ATCTGCAGCC TATATGAAAA TCCAGCAGTA TGACAAGGCA
    CTGGATGATG CAATCAAAGC TCGACTTCTC AATCCCAAGT GGCCAAAGGC ATACTTCCGA
    CAGGGTGTTG CCCTCCAGTA CCTCGGACGT CATGCCGATG CCCTGGCAGC CTTTGCGTCT
    GGGTTGGCTC AGGACCCCAA GAGTCTCCAG CTTCTGGTGG GGATGGTAGA AGCAGCCATG
    AAATCTCCCA TGAGAGACTC CCTCGAACCC ACTTATCAGC AGCTTCAGAA AATGAAACTG
    GACAAGAGCC CCTTTGTGGT TGTCTCTGTG GTTGGACAGG AGCTCCTGAC AGCTGGCCAT
    CACGGGGCCT CTGTGGTTGT CTTAGAAGCC GCTCTGAAGA TTGGCACCTG CAGCCTCAAG
    CTGAGAGGTT CCGTTTTCTC TGCACTGAGC AGTGCTTACT GGTCTCTCGG GAACACAGAG
    AAGAGCACTG GATACATGCA GCAGGACTTG GATGTAGCCA AGACCTTAGG TGACCAGACA
    GGAGAATGCC GAGCTCATGG GAACCTGGGC TCTGCATTCT TCTCCAAAGG AAATTACCGG
    GAGGCTCTCA CTAACCACAG GCATCAGTTG GTGCTTGCCA TGAAACTCAA GGATCGAGAG
    GCAGCTTCAT CAGCCCTGAG CAGTCTGGGC CACGTGTACA CAGCCATTGG AGATTACCCC
    AATGCACTGG CCAGTCACAA ACAGTGTGTT CTTCTCGCCA AGCAATCCAA AGATGAGCTT
    TCTGAAGCCC GAGAACTCGG CAACATGGGA GCTGTGTATA TTGCCATGGG TGACTTTGAG
    AATGCTGTGC AGTGCCACGA GCAGCATCTG AAGATAGCCA AGGACCTGGG GAACAAGCGA
    GAAGAAGCCC GGGCCTACAG CAATCTGGGC AGTGCCTATC ACTACAGGAG GAACTTTGAC
    AAGGCCATGT CTTACCACAA TTACGTGCTG GAGCTGGCGC AGGAGCTGAT GGAGAAGGCC
    ATTGAGATGC GGGCCTACGC TGGCCTGGGC CACGCTGCCA GGTGCATGCA GGATTTGGAG
    AGGGCCAAAC AGTACCACGA GCAGCAGCTG GGCATTGCTG AGGATCTCAA GGACCGGGCT
    GCAGAGGGGC GAGCGTCTTC CAATCTGGGA ATCATACACC AGATGAAAGG TGATTATGAC
    ACTGCACTGA AACTCCACAA GACCCACTTG TGCATCGCCC AGGAGCTGAG TGACTACGCG
    GCCCAGGGCC GCGCCTATGG AAACATGGGC AACGCCTACA ATGCTCTGGG CATGTACGAC
    CAAGCGGTCA AGTACCACCG GCAGGAGCTA CAGATCTCCA TGGAAGTGAA CGACCGTGCC
    TCACAGGCAT CCACGCACGG GAACCTCGCT GTGGCCTACC AGGCGCTGGG CGCCCACGAC
    CGGGCCCTGC AGCACTATCA GAACCATTTG AACATCGCCC GGGAGCTGCG AGACATCCAG
    AGCGAGGCGC GGGCTCTCAG CAACCTGGGC AACTTCCACT GCTCTCGAGG GGAGTACGTC
    CAGGCCGCCC CCTATTACGA ACAGTACCTC CGGCTTGCTC CTGACCTTCA AGACATGGAG
    GGAGAAGGGA AGGTCTGCCA CAACCTCGGC TATGCCCATT ACTGCCTTGG AAATTACCAG
    GAGGCGGTAA AGTACTATGA GCAGGATCTG GCCCTGGCCA AAGACCTTCA CGACAAATTG
    AGTCAAGCAA AAGCTTACTG CAACTTGGGC TTGGCATTCA AGGCTCTGCT GAATTTCAGC
    AAAGCTGAAG AGTGTCAAAA GTACCTACTG TCCCTAGCCC AGTCCCTGAA TAATTCCCAG
    GCTAAATTTA GAGCCCTGGG GAACCTGGGC GATATATTCA TCTGTAAAAA AGATATCAAT
    GGTGCAATAA AATTCTATGA GCAGCAGCTG GGCTTGGCTC ACCACGTGAA GGACAGGAGA
    CTGGAGGCCA GTGCATATGC AGCCCTGGGC ACTGCATACC GAATGATCCA GAAACACGAC
    AAGGCCTTGG GTTATCACAC ACAGGAGCTG GAAGTATATC AGGAGCTGAG TGACCTGCCG
    GGAGAGTGCA GAGCTCATGG GCACCTGGCC GCAGTCTACA TGGCCCTGGG GAAGTACACA
    ATGGCTTTCA AGTGTTACGA AGAGCAGCTG GATCTAGGGC AGAAGCTGAA GGACCCAAGT
    CTCGAAGCCC AGGTCTATGG CAACATGGGC ATCACAAAGA TGAACATGAA TGTGATGGAA
    GAAGCCATCG GCTACTTCGA GCAGCAGCTG GCCATGCTGC AGCAGCTGAG TGGGAACGAG
    TCTGTGCTTG ACAGGGGCCG GGCCTATGGC AACCTGGGGG ACTGCTACGA GGCCCTGGGT
    GACTACGAGG AAGCTATCAA GTACTATGAA CAGTATTTAT CTGTTGCCCA GAGTCTGAAT
    CGCATGCAAG ACCAAGCCAA GGCTTACCGA GGCCTGGGAA ATGGACACAG GGCAATGGGG
    AGCTTGCAGC AAGCCCTGGT GTGCTTTGAA AAGAGGCTTG TGGTCGCCCA TGAGCTCGGG
    GAGGCCTTTA ATAAAGCCCA GGCCTATGGC GAGCTGGGAA GTCTGCACAG CCAATTAGGA
    AATTATGAAC AAGCCATTTC CTGCCTTGAA CGCCAGCTGA ACATTGCTAG AGAGATGAAG
    GACCGAGCTC TGGAAAGTGA CGCGGCCTGT GGCCTGGGGG GTGTGTACCA GCAGATGGGG
    GAGTACGATA CAGCCCTGCA GTACCACCAG TTGGATCTGC AGATTGCCGA GGAGACCAAC
    AACCCCACGT GCCAGGGCCG GGCCTACGGG AACCTGGGCC TGACCTACGA GTCCCTGGGC
    ACCTTTGAGA GAGCCGTGGT CTATCAGGAA CAGCACTTGA GCATCGCTGC ACAGATGAAC
    GACTTGGTGG CCAAGACGGT GTCTTACAGC AGCCTTGGAA GGACGCATCA CGCTCTGCAG
    AACTACTCCC AGGCAGTCAT GTACTTGCAG GAAGGTTTAA GGTTAGCAGA GCAGCTGGGC
    CGAAGAGAAG ATGAAGCCAA GATTCGCCAT GGTCTTGGCC TCTCCCTCTG GGCGAGCGGG
    AATCTGGAGG AGGCCCAACA TCAGCTCTAC CGGGCATCAG CCTTGTTTGA GACGATCCGA
    CACGAGGCGC AGCTGAGCAC AGACTACAAG CTCTCCCTCT TCGACCTGCA GACGTCCTCC
    TACCAGGCCT TGCAGCGAGT GCTCGTCAGC CTCGGCCATC ATGATGAAGC TCTGGCTGTG
    GCAGAAAGAG GACGGACGAG GGCATTTGCA GATCTCCTGG TGGAACGGCA AACAGGACAG
    CAGGACTCTG ACCCCTACTC CCCGGTCACT ATCGATCAGA TCTTAGAGAT GGTTAACGGC
    CAGAGGGGAC TTGTGCTTTA CTACTCCCTG GCGGCGGGCT ACCTGTACAG CTGGCTGCTT
    GCTCCTGGGG CAGGAATTCT GAAGTTTCAT GAGCACTACC TGGGAGACAG CACAGTGGGA
    AGCCCCAGTG ACCCCCAGAC GGGCGGCAGC GCGGCCCTTC CAACTGCCGC GAGCTCGGCC
    CTGGAGCAGC ACGTTGCCAG CGTGCGGGAG GCCCTGGGCG TGGAGTCCTA CTGCTCGAGG
    GCCTGTGCCA GCAGTGAGAC CGAGAGTGAA GCTGGAGACA TCATGGACCA GCAGTTCGAG
    GAGATGAACA ACAGGCTCAA CTCGGTCACC GACCCCACTG GCTTTCTTCG GATGGTTCGC
    CGCAACAACC TCTTTAACAG GAGCTGCCAG AGCATGACCA GCCTGGTCGG CAACACCGCG
    TCGCCCATCA AGGACGGGAC CTCCTCTCTT CCCAGGAGGC AGAGCTCATT CGCCAGGCCG
    CCGCTCCGCG CCCTGTACGA CCTGCTTGTG GCACCCATGG AAGGGGGCCT GATGCACTCC
    AGCGGCCCAG TGGGCCGGCA CCGGCAGCTG GTCCTGGTCC TGGAGGGGGA CCTCTACCTC
    ATCCCGTTCG CCCTCCTGAA GGGAAGCTCC TCCAACGAGT ACCTGTACGA GCGCTTTGCC
    CTCATTGCCG TCCCCTCCAT CCGCTCCCTC AGCCCGCAGG CCAAGTCTCA CCTGAGGAAG
    AGCCCGCCCG CCTATTCCAG CTCCACGTCC ATGGCGGCGG TGGTCGGCAA CCCCAAGCTG
    CCGTCGGCGG TGATGGACAG GTGGCTGTGG GGCCCGATGC CGTCAGCCGA GGAGGAAGCC
    TACATGGTGT CCGAGCTGCT GGGCTGCCAG CCGCTGGTGG GCAGCGTGGC CACCAAAGAG
    CGGGTCATGA GTGCCCTGAC GCAGGCCGAG TGCGTCCACT TCGCCACCCA CATCTCTTGG
    AAACTCTCGG CCTTGGTCCT CACGCCCAGT GTGGACGGCG CCCCTGCAGG CGGCAAGAGC
    TCCTTCGGCC ACCCCTACAC CATCCCTGAG TCCCTGCGGG TGCAGGACGA CGCCAGCGAC
    GGCGAGAGCA TCTCAGACTG CCCACCGCTG CAGGAGCTGC TGCTCACGGC CGCCGACATC
    CTGGACCTGC GGCTGCCCGT CAAGCTGGTG GTGCTCGGCT CCTCCCAGGA GTCCAGCAGC
    AGGATCACGG CCGATGGGGT GGTGGCGCTG ACTCGGGCCT TCCTGGCGGC GGGTGCTCAG
    TGTGTCCTCG TGTCTCTGTG GCCTGTGCCT GTGGCCGCTT CCAAGATGTT CCTGCACGCC
    CTGTACTCGT CCCTGCTGAA CGGCCTGAAG GCCAGTGCGG CACTGGGCGA GGCCATGAAG
    GTGGTGCAGA GCAGCAAGGC CTTCTCACAC CCCTCCAACT GGGCAGGGTT CATGCTTATC
    GGCAGCGATG TGAAGCTCAA CAGTCCCTCT TCGCTCATCG GTCAGGCCCT CACCGAGATC
    CTCCAGCACC CGGAGCGCGC GCGGGACGCC CTGCGCGTGC TGCTGCACCT GGTGGAGAAG
    TCGCTGCAGC GCATTCAGAA CGGGCAGCGC AACGCCATGT ACACGTCCCA GCAGAGCGTG
    GAGAACAAAG TGGGCGGCAT CCCTGGCTGG CAGGCCCTCC TCACCGCTGT GGGCTTCCGG
    CTGGACCCCC CGGCCAGCGG CCTGCCAGCA GCCGTCTTCT TCCCAACCTC CGACCCGGGC
    GACCGGCTCC AGCAGTGCAG CAGCACCGTC CAGTCCCTGC TGGGTCTGCC CAACCCTGCC
    CTCCAAGCCC TCTGCAAGCT CATCACGGCC TCGGAGACAG GCGAGCAGCT CATCAGCCGG
    GCTGTTAAAA ATATGGTTGG AATGCTCCAC CAGGTGCTGG TGCAGCTGCA GGCCGGCGAG
    AAGGAGCAGG ACTTCGCCTC AGCGCCCATC CAGGTCTCCA TCAGCGTCCA GCTGTGGCGG
    CTCCCCGGCT GCCACGAGTT CCTGGCGGCT CTAGGTTTTG ATCTCTGTGA GGTCGGTCAG
    GAGGAAGTAA TCCTGAAAAC CGGGAAACAA GCCAACCGAC GGACCATGCA CTTTGCGCTC
    CAGGCCCTGC TCGCGCTCTT CGATTCCACG GAGCTGCCCA AGCGCCTCAG CCTGGACAGC
    TCGTCCTCCC TGGAGTCGCT GGCCTCGGCC CAGTCTGTCT CCAACGCTCT GCCCCTGGGC
    TTCCAGCACC CTCCTTTCTC CCCGACCGGG GCAGAGAGCA TAGCCTCAGA CGCCATCTCT
    GTGTACAGCC TGAGCTCCAT TGCCTCCTCC ATGAGCTTCG TCTCCAAGCC TGACGGGGGG
    TCAGAAGGCG CGGTCCCTCG GGGACGGCAG GACTACGAGC GGTCCAAGAA CACCTACCCC
    CCTCGCGCTG CCCTGCCCCG GAGCCCGCTG TCCCCGCCTG CCCGCCCTGC AGGCGCCAAG
    GACGAGGAGG AGTACGAGGG GTTCTCCATC ATTAGCACAG AGCCCTTGGC GGCCTCCCAG
    GAGGACCCAC CGACGGGCTT CTCACCCGAC CACAAGCAGT CCCTCGTGGG GACAGCGGGC
    GGCGTCAAAG TCTCGGTGAG CTCAAGCACT CCAAACTCTC CCGTTAAAAT GACTCTGATT
    CCCAGCCCAA ACTCACCCTT CCAGAAAGTT GGAAAACTAG CAAGTTCAGA TACGGGAGAA
    TCAGACCAGT CGAGCACAGA AACAGATAGT ACCGTGAAGT CCCAAGAAGA AAGTAACTCA
    AAGCTTGACC CTCAAGAGCT GGCACAGAAG ATTCTCGAGG AGACACAAAG CCATCTCATG
    GCAGTGGAGC GTCTTCAGAG GGGTGGCGGT CAGGGCGCCA GTTCTGAAGA TGGCATCTCT
    GTGCCCAACA GCGCTGCCGT CTTCCGAGCG TCCGAAACCA GCGCCTTCAG CAGGCCCGCC
    CTCTCCCACC AGAAGAGCCA GGCGGCGTCG TCGTCGTCGC CGGTCACCAT CAAACCAAAG
    CCCCCAGCCA GGAGCTCCTC CCTCCCCAAG GTGAGCTCGG GGTACAGCAG CCCCACCGCC
    TCCGAGGCGT CCGCCAAAGA CAGCCCGAGC CAGCACAGCG GCCGGCCGTC GCCCGGCTCC
    GACTCGCAGA CCTCCCTGAG CGACCAGCCT CTCTTCAAGC TGAAGTACCC CAGCTCCCCC
    TACAGCGCTC ACATCTCCAA ATCTCCAAGG AACATGTCCC CCAGCTCAGG CCACCAGTCT
    CCCGCGGGAA GCGCACCCTC CCCGGCTCTC TCCTACTCCT CGGCGGGCTC CGCGCGGTCC
    AGCCCCGCCG ACCACCCCGA CCTCGACAAG CTGAAGATGG CGGCCATCGA CGAGAAGGTG
    CAGGCCGTCC ACAACCTGAA GATGTTCTGG CAGAGCACAC CCCAGCACTC CACGGGACCA
    ATGAAAATAC TCCGGGGGGC CCCTGGAACC ATGACTTCCA AGAGAGATGT CCTCAGCCTA
    TTGAATTTGT CACCTCGGCA CAACAAGAAG GAGGAGGGCG CAGACAAGCT GGAACTCAAG
    GAGCTGTCTT TGCAGAGACG TGGAGAAACC CCCCCCAAAG CCCCGCCCAA CGGACACTGG
    CGCACGGAGA CCACCTCGCT GAGCACGCTG CCGCTGCCCG CCCGCCCACC TGCCCCGGCT
    CCCACGCGCC CTCTCAGACT TCCTTCTGGA AACGGCTACA AGTTTCTGTC CCCTGGAAGA
    TTTTTTCCTT CCTCTAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93256
    Clone ID Related Accession (Same CDS sequence) XM_617836.9 , XM_015475572.1
    Accession Version XM_015475572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tetratricopeptide repeat protein 28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGAAGAATA TTCCTCTCTT TGGTGCTAAG ACTATTGGCC GGAGAAGTCC TGATGGACCA 
    GTGCTGAGCA AAGCTGAATT TGTTGAGAAA GTTCGTCAGA GTAATCAGGC CTGTCATGAT
    GGAGATTTCC ACACAGCTAT TGTTCTCTAC AATGAAGCCC TGGCTGTTGA CCCTCAGAAC
    TGTATCTTAT ACAGCAATAG ATCTGCAGCC TATATGAAAA TCCAGCAGTA TGACAAGGCA
    CTGGATGATG CAATCAAAGC TCGACTTCTC AATCCCAAGT GGCCAAAGGC ATACTTCCGA
    CAGGGTGTTG CCCTCCAGTA CCTCGGACGT CATGCCGATG CCCTGGCAGC CTTTGCGTCT
    GGGTTGGCTC AGGACCCCAA GAGTCTCCAG CTTCTGGTGG GGATGGTAGA AGCAGCCATG
    AAATCTCCCA TGAGAGACTC CCTCGAACCC ACTTATCAGC AGCTTCAGAA AATGAAACTG
    GACAAGAGCC CCTTTGTGGT TGTCTCTGTG GTTGGACAGG AGCTCCTGAC AGCTGGCCAT
    CACGGGGCCT CTGTGGTTGT CTTAGAAGCC GCTCTGAAGA TTGGCACCTG CAGCCTCAAG
    CTGAGAGGTT CCGTTTTCTC TGCACTGAGC AGTGCTTACT GGTCTCTCGG GAACACAGAG
    AAGAGCACTG GATACATGCA GCAGGACTTG GATGTAGCCA AGACCTTAGG TGACCAGACA
    GGAGAATGCC GAGCTCATGG GAACCTGGGC TCTGCATTCT TCTCCAAAGG AAATTACCGG
    GAGGCTCTCA CTAACCACAG GCATCAGTTG GTGCTTGCCA TGAAACTCAA GGATCGAGAG
    GCAGCTTCAT CAGCCCTGAG CAGTCTGGGC CACGTGTACA CAGCCATTGG AGATTACCCC
    AATGCACTGG CCAGTCACAA ACAGTGTGTT CTTCTCGCCA AGCAATCCAA AGATGAGCTT
    TCTGAAGCCC GAGAACTCGG CAACATGGGA GCTGTGTATA TTGCCATGGG TGACTTTGAG
    AATGCTGTGC AGTGCCACGA GCAGCATCTG AAGATAGCCA AGGACCTGGG GAACAAGCGA
    GAAGAAGCCC GGGCCTACAG CAATCTGGGC AGTGCCTATC ACTACAGGAG GAACTTTGAC
    AAGGCCATGT CTTACCACAA TTACGTGCTG GAGCTGGCGC AGGAGCTGAT GGAGAAGGCC
    ATTGAGATGC GGGCCTACGC TGGCCTGGGC CACGCTGCCA GGTGCATGCA GGATTTGGAG
    AGGGCCAAAC AGTACCACGA GCAGCAGCTG GGCATTGCTG AGGATCTCAA GGACCGGGCT
    GCAGAGGGGC GAGCGTCTTC CAATCTGGGA ATCATACACC AGATGAAAGG TGATTATGAC
    ACTGCACTGA AACTCCACAA GACCCACTTG TGCATCGCCC AGGAGCTGAG TGACTACGCG
    GCCCAGGGCC GCGCCTATGG AAACATGGGC AACGCCTACA ATGCTCTGGG CATGTACGAC
    CAAGCGGTCA AGTACCACCG GCAGGAGCTA CAGATCTCCA TGGAAGTGAA CGACCGTGCC
    TCACAGGCAT CCACGCACGG GAACCTCGCT GTGGCCTACC AGGCGCTGGG CGCCCACGAC
    CGGGCCCTGC AGCACTATCA GAACCATTTG AACATCGCCC GGGAGCTGCG AGACATCCAG
    AGCGAGGCGC GGGCTCTCAG CAACCTGGGC AACTTCCACT GCTCTCGAGG GGAGTACGTC
    CAGGCCGCCC CCTATTACGA ACAGTACCTC CGGCTTGCTC CTGACCTTCA AGACATGGAG
    GGAGAAGGGA AGGTCTGCCA CAACCTCGGC TATGCCCATT ACTGCCTTGG AAATTACCAG
    GAGGCGGTAA AGTACTATGA GCAGGATCTG GCCCTGGCCA AAGACCTTCA CGACAAATTG
    AGTCAAGCAA AAGCTTACTG CAACTTGGGC TTGGCATTCA AGGCTCTGCT GAATTTCAGC
    AAAGCTGAAG AGTGTCAAAA GTACCTACTG TCCCTAGCCC AGTCCCTGAA TAATTCCCAG
    GCTAAATTTA GAGCCCTGGG GAACCTGGGC GATATATTCA TCTGTAAAAA AGATATCAAT
    GGTGCAATAA AATTCTATGA GCAGCAGCTG GGCTTGGCTC ACCACGTGAA GGACAGGAGA
    CTGGAGGCCA GTGCATATGC AGCCCTGGGC ACTGCATACC GAATGATCCA GAAACACGAC
    AAGGCCTTGG GTTATCACAC ACAGGAGCTG GAAGTATATC AGGAGCTGAG TGACCTGCCG
    GGAGAGTGCA GAGCTCATGG GCACCTGGCC GCAGTCTACA TGGCCCTGGG GAAGTACACA
    ATGGCTTTCA AGTGTTACGA AGAGCAGCTG GATCTAGGGC AGAAGCTGAA GGACCCAAGT
    CTCGAAGCCC AGGTCTATGG CAACATGGGC ATCACAAAGA TGAACATGAA TGTGATGGAA
    GAAGCCATCG GCTACTTCGA GCAGCAGCTG GCCATGCTGC AGCAGCTGAG TGGGAACGAG
    TCTGTGCTTG ACAGGGGCCG GGCCTATGGC AACCTGGGGG ACTGCTACGA GGCCCTGGGT
    GACTACGAGG AAGCTATCAA GTACTATGAA CAGTATTTAT CTGTTGCCCA GAGTCTGAAT
    CGCATGCAAG ACCAAGCCAA GGCTTACCGA GGCCTGGGAA ATGGACACAG GGCAATGGGG
    AGCTTGCAGC AAGCCCTGGT GTGCTTTGAA AAGAGGCTTG TGGTCGCCCA TGAGCTCGGG
    GAGGCCTTTA ATAAAGCCCA GGCCTATGGC GAGCTGGGAA GTCTGCACAG CCAATTAGGA
    AATTATGAAC AAGCCATTTC CTGCCTTGAA CGCCAGCTGA ACATTGCTAG AGAGATGAAG
    GACCGAGCTC TGGAAAGTGA CGCGGCCTGT GGCCTGGGGG GTGTGTACCA GCAGATGGGG
    GAGTACGATA CAGCCCTGCA GTACCACCAG TTGGATCTGC AGATTGCCGA GGAGACCAAC
    AACCCCACGT GCCAGGGCCG GGCCTACGGG AACCTGGGCC TGACCTACGA GTCCCTGGGC
    ACCTTTGAGA GAGCCGTGGT CTATCAGGAA CAGCACTTGA GCATCGCTGC ACAGATGAAC
    GACTTGGTGG CCAAGACGGT GTCTTACAGC AGCCTTGGAA GGACGCATCA CGCTCTGCAG
    AACTACTCCC AGGCAGTCAT GTACTTGCAG GAAGGTTTAA GGTTAGCAGA GCAGCTGGGC
    CGAAGAGAAG ATGAAGCCAA GATTCGCCAT GGTCTTGGCC TCTCCCTCTG GGCGAGCGGG
    AATCTGGAGG AGGCCCAACA TCAGCTCTAC CGGGCATCAG CCTTGTTTGA GACGATCCGA
    CACGAGGCGC AGCTGAGCAC AGACTACAAG CTCTCCCTCT TCGACCTGCA GACGTCCTCC
    TACCAGGCCT TGCAGCGAGT GCTCGTCAGC CTCGGCCATC ATGATGAAGC TCTGGCTGTG
    GCAGAAAGAG GACGGACGAG GGCATTTGCA GATCTCCTGG TGGAACGGCA AACAGGACAG
    CAGGACTCTG ACCCCTACTC CCCGGTCACT ATCGATCAGA TCTTAGAGAT GGTTAACGGC
    CAGAGGGGAC TTGTGCTTTA CTACTCCCTG GCGGCGGGCT ACCTGTACAG CTGGCTGCTT
    GCTCCTGGGG CAGGAATTCT GAAGTTTCAT GAGCACTACC TGGGAGACAG CACAGTGGGA
    AGCCCCAGTG ACCCCCAGAC GGGCGGCAGC GCGGCCCTTC CAACTGCCGC GAGCTCGGCC
    CTGGAGCAGC ACGTTGCCAG CGTGCGGGAG GCCCTGGGCG TGGAGTCCTA CTGCTCGAGG
    GCCTGTGCCA GCAGTGAGAC CGAGAGTGAA GCTGGAGACA TCATGGACCA GCAGTTCGAG
    GAGATGAACA ACAGGCTCAA CTCGGTCACC GACCCCACTG GCTTTCTTCG GATGGTTCGC
    CGCAACAACC TCTTTAACAG GAGCTGCCAG AGCATGACCA GCCTGGTCGG CAACACCGCG
    TCGCCCATCA AGGACGGGAC CTCCTCTCTT CCCAGGAGGC AGAGCTCATT CGCCAGGCCG
    CCGCTCCGCG CCCTGTACGA CCTGCTTGTG GCACCCATGG AAGGGGGCCT GATGCACTCC
    AGCGGCCCAG TGGGCCGGCA CCGGCAGCTG GTCCTGGTCC TGGAGGGGGA CCTCTACCTC
    ATCCCGTTCG CCCTCCTGAA GGGAAGCTCC TCCAACGAGT ACCTGTACGA GCGCTTTGCC
    CTCATTGCCG TCCCCTCCAT CCGCTCCCTC AGCCCGCAGG CCAAGTCTCA CCTGAGGAAG
    AGCCCGCCCG CCTATTCCAG CTCCACGTCC ATGGCGGCGG TGGTCGGCAA CCCCAAGCTG
    CCGTCGGCGG TGATGGACAG GTGGCTGTGG GGCCCGATGC CGTCAGCCGA GGAGGAAGCC
    TACATGGTGT CCGAGCTGCT GGGCTGCCAG CCGCTGGTGG GCAGCGTGGC CACCAAAGAG
    CGGGTCATGA GTGCCCTGAC GCAGGCCGAG TGCGTCCACT TCGCCACCCA CATCTCTTGG
    AAACTCTCGG CCTTGGTCCT CACGCCCAGT GTGGACGGCG CCCCTGCAGG CGGCAAGAGC
    TCCTTCGGCC ACCCCTACAC CATCCCTGAG TCCCTGCGGG TGCAGGACGA CGCCAGCGAC
    GGCGAGAGCA TCTCAGACTG CCCACCGCTG CAGGAGCTGC TGCTCACGGC CGCCGACATC
    CTGGACCTGC GGCTGCCCGT CAAGCTGGTG GTGCTCGGCT CCTCCCAGGA GTCCAGCAGC
    AGGATCACGG CCGATGGGGT GGTGGCGCTG ACTCGGGCCT TCCTGGCGGC GGGTGCTCAG
    TGTGTCCTCG TGTCTCTGTG GCCTGTGCCT GTGGCCGCTT CCAAGATGTT CCTGCACGCC
    CTGTACTCGT CCCTGCTGAA CGGCCTGAAG GCCAGTGCGG CACTGGGCGA GGCCATGAAG
    GTGGTGCAGA GCAGCAAGGC CTTCTCACAC CCCTCCAACT GGGCAGGGTT CATGCTTATC
    GGCAGCGATG TGAAGCTCAA CAGTCCCTCT TCGCTCATCG GTCAGGCCCT CACCGAGATC
    CTCCAGCACC CGGAGCGCGC GCGGGACGCC CTGCGCGTGC TGCTGCACCT GGTGGAGAAG
    TCGCTGCAGC GCATTCAGAA CGGGCAGCGC AACGCCATGT ACACGTCCCA GCAGAGCGTG
    GAGAACAAAG TGGGCGGCAT CCCTGGCTGG CAGGCCCTCC TCACCGCTGT GGGCTTCCGG
    CTGGACCCCC CGGCCAGCGG CCTGCCAGCA GCCGTCTTCT TCCCAACCTC CGACCCGGGC
    GACCGGCTCC AGCAGTGCAG CAGCACCGTC CAGTCCCTGC TGGGTCTGCC CAACCCTGCC
    CTCCAAGCCC TCTGCAAGCT CATCACGGCC TCGGAGACAG GCGAGCAGCT CATCAGCCGG
    GCTGTTAAAA ATATGGTTGG AATGCTCCAC CAGGTGCTGG TGCAGCTGCA GGCCGGCGAG
    AAGGAGCAGG ACTTCGCCTC AGCGCCCATC CAGGTCTCCA TCAGCGTCCA GCTGTGGCGG
    CTCCCCGGCT GCCACGAGTT CCTGGCGGCT CTAGGTTTTG ATCTCTGTGA GGTCGGTCAG
    GAGGAAGTAA TCCTGAAAAC CGGGAAACAA GCCAACCGAC GGACCATGCA CTTTGCGCTC
    CAGGCCCTGC TCGCGCTCTT CGATTCCACG GAGCTGCCCA AGCGCCTCAG CCTGGACAGC
    TCGTCCTCCC TGGAGTCGCT GGCCTCGGCC CAGTCTGTCT CCAACGCTCT GCCCCTGGGC
    TTCCAGCACC CTCCTTTCTC CCCGACCGGG GCAGAGAGCA TAGCCTCAGA CGCCATCTCT
    GTGTACAGCC TGAGCTCCAT TGCCTCCTCC ATGAGCTTCG TCTCCAAGCC TGACGGGGGG
    TCAGAAGGCG CGGTCCCTCG GGGACGGCAG GACTACGAGC GGTCCAAGAA CACCTACCCC
    CCTCGCGCTG CCCTGCCCCG GAGCCCGCTG TCCCCGCCTG CCCGCCCTGC AGGCGCCAAG
    GACGAGGAGG AGTACGAGGG GTTCTCCATC ATTAGCACAG AGCCCTTGGC GGCCTCCCAG
    GAGGACCCAC CGACGGGCTT CTCACCCGAC CACAAGCAGT CCCTCGTGGG GACAGCGGGC
    GGCGTCAAAG TCTCGGTGAG CTCAAGCACT CCAAACTCTC CCGTTAAAAT GACTCTGATT
    CCCAGCCCAA ACTCACCCTT CCAGAAAGTT GGAAAACTAG CAAGTTCAGA TACGGGAGAA
    TCAGACCAGT CGAGCACAGA AACAGATAGT ACCGTGAAGT CCCAAGAAGA AAGTAACTCA
    AAGCTTGACC CTCAAGAGCT GGCACAGAAG ATTCTCGAGG AGACACAAAG CCATCTCATG
    GCAGTGGAGC GTCTTCAGAG GGGTGGCGGT CAGGGCGCCA GTTCTGAAGA TGGCATCTCT
    GTGCCCAACA GCGCTGCCGT CTTCCGAGCG TCCGAAACCA GCGCCTTCAG CAGGCCCGCC
    CTCTCCCACC AGAAGAGCCA GGCGGCGTCG TCGTCGTCGC CGGTCACCAT CAAACCAAAG
    CCCCCAGCCA GGAGCTCCTC CCTCCCCAAG GTGAGCTCGG GGTACAGCAG CCCCACCGCC
    TCCGAGGCGT CCGCCAAAGA CAGCCCGAGC CAGCACAGCG GCCGGCCGTC GCCCGGCTCC
    GACTCGCAGA CCTCCCTGAG CGACCAGCCT CTCTTCAAGC TGAAGTACCC CAGCTCCCCC
    TACAGCGCTC ACATCTCCAA ATCTCCAAGG AACATGTCCC CCAGCTCAGG CCACCAGTCT
    CCCGCGGGAA GCGCACCCTC CCCGGCTCTC TCCTACTCCT CGGCGGGCTC CGCGCGGTCC
    AGCCCCGCCG ACCACCCCGA CCTCGACAAG CTGAAGATGG CGGCCATCGA CGAGAAGGTG
    CAGGCCGTCC ACAACCTGAA GATGTTCTGG CAGAGCACAC CCCAGCACTC CACGGGACCA
    ATGAAAATAC TCCGGGGGGC CCCTGGAACC ATGACTTCCA AGAGAGATGT CCTCAGCCTA
    TTGAATTTGT CACCTCGGCA CAACAAGAAG GAGGAGGGCG CAGACAAGCT GGAACTCAAG
    GAGCTGTCTT TGCAGAGACG TGGAGAAACC CCCCCCAAAG CCCCGCCCAA CGGACACTGG
    CGCACGGAGA CCACCTCGCT GAGCACGCTG CCGCTGCCCG CCCGCCCACC TGCCCCGGCT
    CCCACGCGCC CTCTCAGACT TCCTTCTGGA AACGGCTACA AGTTTCTGTC CCCTGGAAGA
    TTTTTTCCTT CCTCTAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331058.1
    CDS1..7344
    Translation

    Target ORF information:

    RefSeq Version XM_015475572.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 28 (TTC28), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGAAGAATA TTCCTCTCTT TGGTGCTAAG ACTATTGGCC GGAGAAGTCC TGATGGACCA 
    GTGCTGAGCA AAGCTGAATT TGTTGAGAAA GTTCGTCAGA GTAATCAGGC CTGTCATGAT
    GGAGATTTCC ACACAGCTAT TGTTCTCTAC AATGAAGCCC TGGCTGTTGA CCCTCAGAAC
    TGTATCTTAT ACAGCAATAG ATCTGCAGCC TATATGAAAA TCCAGCAGTA TGACAAGGCA
    CTGGATGATG CAATCAAAGC TCGACTTCTC AATCCCAAGT GGCCAAAGGC ATACTTCCGA
    CAGGGTGTTG CCCTCCAGTA CCTCGGACGT CATGCCGATG CCCTGGCAGC CTTTGCGTCT
    GGGTTGGCTC AGGACCCCAA GAGTCTCCAG CTTCTGGTGG GGATGGTAGA AGCAGCCATG
    AAATCTCCCA TGAGAGACTC CCTCGAACCC ACTTATCAGC AGCTTCAGAA AATGAAACTG
    GACAAGAGCC CCTTTGTGGT TGTCTCTGTG GTTGGACAGG AGCTCCTGAC AGCTGGCCAT
    CACGGGGCCT CTGTGGTTGT CTTAGAAGCC GCTCTGAAGA TTGGCACCTG CAGCCTCAAG
    CTGAGAGGTT CCGTTTTCTC TGCACTGAGC AGTGCTTACT GGTCTCTCGG GAACACAGAG
    AAGAGCACTG GATACATGCA GCAGGACTTG GATGTAGCCA AGACCTTAGG TGACCAGACA
    GGAGAATGCC GAGCTCATGG GAACCTGGGC TCTGCATTCT TCTCCAAAGG AAATTACCGG
    GAGGCTCTCA CTAACCACAG GCATCAGTTG GTGCTTGCCA TGAAACTCAA GGATCGAGAG
    GCAGCTTCAT CAGCCCTGAG CAGTCTGGGC CACGTGTACA CAGCCATTGG AGATTACCCC
    AATGCACTGG CCAGTCACAA ACAGTGTGTT CTTCTCGCCA AGCAATCCAA AGATGAGCTT
    TCTGAAGCCC GAGAACTCGG CAACATGGGA GCTGTGTATA TTGCCATGGG TGACTTTGAG
    AATGCTGTGC AGTGCCACGA GCAGCATCTG AAGATAGCCA AGGACCTGGG GAACAAGCGA
    GAAGAAGCCC GGGCCTACAG CAATCTGGGC AGTGCCTATC ACTACAGGAG GAACTTTGAC
    AAGGCCATGT CTTACCACAA TTACGTGCTG GAGCTGGCGC AGGAGCTGAT GGAGAAGGCC
    ATTGAGATGC GGGCCTACGC TGGCCTGGGC CACGCTGCCA GGTGCATGCA GGATTTGGAG
    AGGGCCAAAC AGTACCACGA GCAGCAGCTG GGCATTGCTG AGGATCTCAA GGACCGGGCT
    GCAGAGGGGC GAGCGTCTTC CAATCTGGGA ATCATACACC AGATGAAAGG TGATTATGAC
    ACTGCACTGA AACTCCACAA GACCCACTTG TGCATCGCCC AGGAGCTGAG TGACTACGCG
    GCCCAGGGCC GCGCCTATGG AAACATGGGC AACGCCTACA ATGCTCTGGG CATGTACGAC
    CAAGCGGTCA AGTACCACCG GCAGGAGCTA CAGATCTCCA TGGAAGTGAA CGACCGTGCC
    TCACAGGCAT CCACGCACGG GAACCTCGCT GTGGCCTACC AGGCGCTGGG CGCCCACGAC
    CGGGCCCTGC AGCACTATCA GAACCATTTG AACATCGCCC GGGAGCTGCG AGACATCCAG
    AGCGAGGCGC GGGCTCTCAG CAACCTGGGC AACTTCCACT GCTCTCGAGG GGAGTACGTC
    CAGGCCGCCC CCTATTACGA ACAGTACCTC CGGCTTGCTC CTGACCTTCA AGACATGGAG
    GGAGAAGGGA AGGTCTGCCA CAACCTCGGC TATGCCCATT ACTGCCTTGG AAATTACCAG
    GAGGCGGTAA AGTACTATGA GCAGGATCTG GCCCTGGCCA AAGACCTTCA CGACAAATTG
    AGTCAAGCAA AAGCTTACTG CAACTTGGGC TTGGCATTCA AGGCTCTGCT GAATTTCAGC
    AAAGCTGAAG AGTGTCAAAA GTACCTACTG TCCCTAGCCC AGTCCCTGAA TAATTCCCAG
    GCTAAATTTA GAGCCCTGGG GAACCTGGGC GATATATTCA TCTGTAAAAA AGATATCAAT
    GGTGCAATAA AATTCTATGA GCAGCAGCTG GGCTTGGCTC ACCACGTGAA GGACAGGAGA
    CTGGAGGCCA GTGCATATGC AGCCCTGGGC ACTGCATACC GAATGATCCA GAAACACGAC
    AAGGCCTTGG GTTATCACAC ACAGGAGCTG GAAGTATATC AGGAGCTGAG TGACCTGCCG
    GGAGAGTGCA GAGCTCATGG GCACCTGGCC GCAGTCTACA TGGCCCTGGG GAAGTACACA
    ATGGCTTTCA AGTGTTACGA AGAGCAGCTG GATCTAGGGC AGAAGCTGAA GGACCCAAGT
    CTCGAAGCCC AGGTCTATGG CAACATGGGC ATCACAAAGA TGAACATGAA TGTGATGGAA
    GAAGCCATCG GCTACTTCGA GCAGCAGCTG GCCATGCTGC AGCAGCTGAG TGGGAACGAG
    TCTGTGCTTG ACAGGGGCCG GGCCTATGGC AACCTGGGGG ACTGCTACGA GGCCCTGGGT
    GACTACGAGG AAGCTATCAA GTACTATGAA CAGTATTTAT CTGTTGCCCA GAGTCTGAAT
    CGCATGCAAG ACCAAGCCAA GGCTTACCGA GGCCTGGGAA ATGGACACAG GGCAATGGGG
    AGCTTGCAGC AAGCCCTGGT GTGCTTTGAA AAGAGGCTTG TGGTCGCCCA TGAGCTCGGG
    GAGGCCTTTA ATAAAGCCCA GGCCTATGGC GAGCTGGGAA GTCTGCACAG CCAATTAGGA
    AATTATGAAC AAGCCATTTC CTGCCTTGAA CGCCAGCTGA ACATTGCTAG AGAGATGAAG
    GACCGAGCTC TGGAAAGTGA CGCGGCCTGT GGCCTGGGGG GTGTGTACCA GCAGATGGGG
    GAGTACGATA CAGCCCTGCA GTACCACCAG TTGGATCTGC AGATTGCCGA GGAGACCAAC
    AACCCCACGT GCCAGGGCCG GGCCTACGGG AACCTGGGCC TGACCTACGA GTCCCTGGGC
    ACCTTTGAGA GAGCCGTGGT CTATCAGGAA CAGCACTTGA GCATCGCTGC ACAGATGAAC
    GACTTGGTGG CCAAGACGGT GTCTTACAGC AGCCTTGGAA GGACGCATCA CGCTCTGCAG
    AACTACTCCC AGGCAGTCAT GTACTTGCAG GAAGGTTTAA GGTTAGCAGA GCAGCTGGGC
    CGAAGAGAAG ATGAAGCCAA GATTCGCCAT GGTCTTGGCC TCTCCCTCTG GGCGAGCGGG
    AATCTGGAGG AGGCCCAACA TCAGCTCTAC CGGGCATCAG CCTTGTTTGA GACGATCCGA
    CACGAGGCGC AGCTGAGCAC AGACTACAAG CTCTCCCTCT TCGACCTGCA GACGTCCTCC
    TACCAGGCCT TGCAGCGAGT GCTCGTCAGC CTCGGCCATC ATGATGAAGC TCTGGCTGTG
    GCAGAAAGAG GACGGACGAG GGCATTTGCA GATCTCCTGG TGGAACGGCA AACAGGACAG
    CAGGACTCTG ACCCCTACTC CCCGGTCACT ATCGATCAGA TCTTAGAGAT GGTTAACGGC
    CAGAGGGGAC TTGTGCTTTA CTACTCCCTG GCGGCGGGCT ACCTGTACAG CTGGCTGCTT
    GCTCCTGGGG CAGGAATTCT GAAGTTTCAT GAGCACTACC TGGGAGACAG CACAGTGGGA
    AGCCCCAGTG ACCCCCAGAC GGGCGGCAGC GCGGCCCTTC CAACTGCCGC GAGCTCGGCC
    CTGGAGCAGC ACGTTGCCAG CGTGCGGGAG GCCCTGGGCG TGGAGTCCTA CTGCTCGAGG
    GCCTGTGCCA GCAGTGAGAC CGAGAGTGAA GCTGGAGACA TCATGGACCA GCAGTTCGAG
    GAGATGAACA ACAGGCTCAA CTCGGTCACC GACCCCACTG GCTTTCTTCG GATGGTTCGC
    CGCAACAACC TCTTTAACAG GAGCTGCCAG AGCATGACCA GCCTGGTCGG CAACACCGCG
    TCGCCCATCA AGGACGGGAC CTCCTCTCTT CCCAGGAGGC AGAGCTCATT CGCCAGGCCG
    CCGCTCCGCG CCCTGTACGA CCTGCTTGTG GCACCCATGG AAGGGGGCCT GATGCACTCC
    AGCGGCCCAG TGGGCCGGCA CCGGCAGCTG GTCCTGGTCC TGGAGGGGGA CCTCTACCTC
    ATCCCGTTCG CCCTCCTGAA GGGAAGCTCC TCCAACGAGT ACCTGTACGA GCGCTTTGCC
    CTCATTGCCG TCCCCTCCAT CCGCTCCCTC AGCCCGCAGG CCAAGTCTCA CCTGAGGAAG
    AGCCCGCCCG CCTATTCCAG CTCCACGTCC ATGGCGGCGG TGGTCGGCAA CCCCAAGCTG
    CCGTCGGCGG TGATGGACAG GTGGCTGTGG GGCCCGATGC CGTCAGCCGA GGAGGAAGCC
    TACATGGTGT CCGAGCTGCT GGGCTGCCAG CCGCTGGTGG GCAGCGTGGC CACCAAAGAG
    CGGGTCATGA GTGCCCTGAC GCAGGCCGAG TGCGTCCACT TCGCCACCCA CATCTCTTGG
    AAACTCTCGG CCTTGGTCCT CACGCCCAGT GTGGACGGCG CCCCTGCAGG CGGCAAGAGC
    TCCTTCGGCC ACCCCTACAC CATCCCTGAG TCCCTGCGGG TGCAGGACGA CGCCAGCGAC
    GGCGAGAGCA TCTCAGACTG CCCACCGCTG CAGGAGCTGC TGCTCACGGC CGCCGACATC
    CTGGACCTGC GGCTGCCCGT CAAGCTGGTG GTGCTCGGCT CCTCCCAGGA GTCCAGCAGC
    AGGATCACGG CCGATGGGGT GGTGGCGCTG ACTCGGGCCT TCCTGGCGGC GGGTGCTCAG
    TGTGTCCTCG TGTCTCTGTG GCCTGTGCCT GTGGCCGCTT CCAAGATGTT CCTGCACGCC
    CTGTACTCGT CCCTGCTGAA CGGCCTGAAG GCCAGTGCGG CACTGGGCGA GGCCATGAAG
    GTGGTGCAGA GCAGCAAGGC CTTCTCACAC CCCTCCAACT GGGCAGGGTT CATGCTTATC
    GGCAGCGATG TGAAGCTCAA CAGTCCCTCT TCGCTCATCG GTCAGGCCCT CACCGAGATC
    CTCCAGCACC CGGAGCGCGC GCGGGACGCC CTGCGCGTGC TGCTGCACCT GGTGGAGAAG
    TCGCTGCAGC GCATTCAGAA CGGGCAGCGC AACGCCATGT ACACGTCCCA GCAGAGCGTG
    GAGAACAAAG TGGGCGGCAT CCCTGGCTGG CAGGCCCTCC TCACCGCTGT GGGCTTCCGG
    CTGGACCCCC CGGCCAGCGG CCTGCCAGCA GCCGTCTTCT TCCCAACCTC CGACCCGGGC
    GACCGGCTCC AGCAGTGCAG CAGCACCGTC CAGTCCCTGC TGGGTCTGCC CAACCCTGCC
    CTCCAAGCCC TCTGCAAGCT CATCACGGCC TCGGAGACAG GCGAGCAGCT CATCAGCCGG
    GCTGTTAAAA ATATGGTTGG AATGCTCCAC CAGGTGCTGG TGCAGCTGCA GGCCGGCGAG
    AAGGAGCAGG ACTTCGCCTC AGCGCCCATC CAGGTCTCCA TCAGCGTCCA GCTGTGGCGG
    CTCCCCGGCT GCCACGAGTT CCTGGCGGCT CTAGGTTTTG ATCTCTGTGA GGTCGGTCAG
    GAGGAAGTAA TCCTGAAAAC CGGGAAACAA GCCAACCGAC GGACCATGCA CTTTGCGCTC
    CAGGCCCTGC TCGCGCTCTT CGATTCCACG GAGCTGCCCA AGCGCCTCAG CCTGGACAGC
    TCGTCCTCCC TGGAGTCGCT GGCCTCGGCC CAGTCTGTCT CCAACGCTCT GCCCCTGGGC
    TTCCAGCACC CTCCTTTCTC CCCGACCGGG GCAGAGAGCA TAGCCTCAGA CGCCATCTCT
    GTGTACAGCC TGAGCTCCAT TGCCTCCTCC ATGAGCTTCG TCTCCAAGCC TGACGGGGGG
    TCAGAAGGCG CGGTCCCTCG GGGACGGCAG GACTACGAGC GGTCCAAGAA CACCTACCCC
    CCTCGCGCTG CCCTGCCCCG GAGCCCGCTG TCCCCGCCTG CCCGCCCTGC AGGCGCCAAG
    GACGAGGAGG AGTACGAGGG GTTCTCCATC ATTAGCACAG AGCCCTTGGC GGCCTCCCAG
    GAGGACCCAC CGACGGGCTT CTCACCCGAC CACAAGCAGT CCCTCGTGGG GACAGCGGGC
    GGCGTCAAAG TCTCGGTGAG CTCAAGCACT CCAAACTCTC CCGTTAAAAT GACTCTGATT
    CCCAGCCCAA ACTCACCCTT CCAGAAAGTT GGAAAACTAG CAAGTTCAGA TACGGGAGAA
    TCAGACCAGT CGAGCACAGA AACAGATAGT ACCGTGAAGT CCCAAGAAGA AAGTAACTCA
    AAGCTTGACC CTCAAGAGCT GGCACAGAAG ATTCTCGAGG AGACACAAAG CCATCTCATG
    GCAGTGGAGC GTCTTCAGAG GGGTGGCGGT CAGGGCGCCA GTTCTGAAGA TGGCATCTCT
    GTGCCCAACA GCGCTGCCGT CTTCCGAGCG TCCGAAACCA GCGCCTTCAG CAGGCCCGCC
    CTCTCCCACC AGAAGAGCCA GGCGGCGTCG TCGTCGTCGC CGGTCACCAT CAAACCAAAG
    CCCCCAGCCA GGAGCTCCTC CCTCCCCAAG GTGAGCTCGG GGTACAGCAG CCCCACCGCC
    TCCGAGGCGT CCGCCAAAGA CAGCCCGAGC CAGCACAGCG GCCGGCCGTC GCCCGGCTCC
    GACTCGCAGA CCTCCCTGAG CGACCAGCCT CTCTTCAAGC TGAAGTACCC CAGCTCCCCC
    TACAGCGCTC ACATCTCCAA ATCTCCAAGG AACATGTCCC CCAGCTCAGG CCACCAGTCT
    CCCGCGGGAA GCGCACCCTC CCCGGCTCTC TCCTACTCCT CGGCGGGCTC CGCGCGGTCC
    AGCCCCGCCG ACCACCCCGA CCTCGACAAG CTGAAGATGG CGGCCATCGA CGAGAAGGTG
    CAGGCCGTCC ACAACCTGAA GATGTTCTGG CAGAGCACAC CCCAGCACTC CACGGGACCA
    ATGAAAATAC TCCGGGGGGC CCCTGGAACC ATGACTTCCA AGAGAGATGT CCTCAGCCTA
    TTGAATTTGT CACCTCGGCA CAACAAGAAG GAGGAGGGCG CAGACAAGCT GGAACTCAAG
    GAGCTGTCTT TGCAGAGACG TGGAGAAACC CCCCCCAAAG CCCCGCCCAA CGGACACTGG
    CGCACGGAGA CCACCTCGCT GAGCACGCTG CCGCTGCCCG CCCGCCCACC TGCCCCGGCT
    CCCACGCGCC CTCTCAGACT TCCTTCTGGA AACGGCTACA AGTTTCTGTC CCCTGGAAGA
    TTTTTTCCTT CCTCTAAATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.