SPTBN4 cDNA ORF clone, Bos taurus(cattle)

The following SPTBN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225119 XM_010814782.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa225119 XM_010814783.3
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa239138 NM_001192910.3
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225117 XM_005218990.4
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225118 XM_010814781.3
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa216201 NM_001192910.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa38868 NM_001192910.1
Latest version!
Bos taurus spectrin, beta, non-erythrocytic 4 (SPTBN4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa28665 XM_005218990.3
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa53693 XM_010814780.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa53694 XM_010814781.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa53695 XM_010814783.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa53695 XM_010814782.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225119
    Clone ID Related Accession (Same CDS sequence) XM_010814782.2 , XM_010814783.3
    Accession Version XM_010814782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGTGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGCGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813084.1
    CDS137..3865
    Translation

    Target ORF information:

    RefSeq Version XM_010814782.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGTGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGCGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225119
    Clone ID Related Accession (Same CDS sequence) XM_010814782.2 , XM_010814783.3
    Accession Version XM_010814783.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGTGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGCGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813085.1
    CDS127..3855
    Translation

    Target ORF information:

    RefSeq Version XM_010814783.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGTGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGCGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239138
    Clone ID Related Accession (Same CDS sequence) NM_001192910.3
    Accession Version NM_001192910.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On May 30, 2018 this sequence version replaced NM_001192910.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCAGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG TGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA TGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGC GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179839.1
    CDS1..7695
    Translation

    Target ORF information:

    RefSeq Version NM_001192910.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192910.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCAGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG TGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA TGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGC GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225117
    Clone ID Related Accession (Same CDS sequence) XM_005218990.4
    Accession Version XM_005218990.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCA GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGTGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATATGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGCGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGC CTTCGCTGCC TCAGCCTCGC
    GAACTTCCCC CCGGCCGCCT GCCCAACGGG CTTGAGCCGC CCGAGCGGAC ACCTCGGCCG
    GACCGGCCGC GGGCTCGGGA CCGGCCCAAG CCGAGACGGC GGCCGCGGCC CCGAGAGGGT
    GGTGAGGGCG GGGGAAGCCG GCGCTCGCGC TCCGCCCCGG CCCAGGGCGG CTCCGCCCCC
    GCGCCTCCAC CACCGCCCAC TCATACAGTG CAGCACGAGG GCTTCCTGCT GCGCAAGCGC
    GAGCTCGACG CTAACCGCAA GTCGTCCAAC CGGTCATGGG TGAGCCTGTA CTGCGTGCTC
    AGCAAGGGGG AGCTGGGTTT CTACAAGGAC GCCAAGGGTC CGGCATCCGG GGGCACACAC
    GGCGGGGAGC CGCTGCTCAG CCTGCACAAG GCCACCAGCG AGGTGGCTAG TGACTACAAG
    AAAAAGAAGC ATGTCTTCAA GCTCCAGACC CAGGACGGCA GTGAGTTTTT GCTCCAGGCT
    AAAGATGAGG AGGAGATGAA CGGCTGGCTG GAGGCTGTGG CCGCCTCGGT TGGGGAACAC
    GCCGAGATCG CCCGCTGGGG CCAGACACAA CCCACCACAT CGTCCACAGA CGAGGGCAAC
    CCCAAGCGGG AGGCAGGGGA GCGCAGGGCC AGCGGGCGCC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219047.1
    CDS3..7850
    Translation

    Target ORF information:

    RefSeq Version XM_005218990.4
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218990.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCA GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGTGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATATGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGCGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGC CTTCGCTGCC TCAGCCTCGC
    GAACTTCCCC CCGGCCGCCT GCCCAACGGG CTTGAGCCGC CCGAGCGGAC ACCTCGGCCG
    GACCGGCCGC GGGCTCGGGA CCGGCCCAAG CCGAGACGGC GGCCGCGGCC CCGAGAGGGT
    GGTGAGGGCG GGGGAAGCCG GCGCTCGCGC TCCGCCCCGG CCCAGGGCGG CTCCGCCCCC
    GCGCCTCCAC CACCGCCCAC TCATACAGTG CAGCACGAGG GCTTCCTGCT GCGCAAGCGC
    GAGCTCGACG CTAACCGCAA GTCGTCCAAC CGGTCATGGG TGAGCCTGTA CTGCGTGCTC
    AGCAAGGGGG AGCTGGGTTT CTACAAGGAC GCCAAGGGTC CGGCATCCGG GGGCACACAC
    GGCGGGGAGC CGCTGCTCAG CCTGCACAAG GCCACCAGCG AGGTGGCTAG TGACTACAAG
    AAAAAGAAGC ATGTCTTCAA GCTCCAGACC CAGGACGGCA GTGAGTTTTT GCTCCAGGCT
    AAAGATGAGG AGGAGATGAA CGGCTGGCTG GAGGCTGTGG CCGCCTCGGT TGGGGAACAC
    GCCGAGATCG CCCGCTGGGG CCAGACACAA CCCACCACAT CGTCCACAGA CGAGGGCAAC
    CCCAAGCGGG AGGCAGGGGA GCGCAGGGCC AGCGGGCGCC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225118
    Clone ID Related Accession (Same CDS sequence) XM_010814781.3
    Accession Version XM_010814781.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCA GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGTGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATATGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGCGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGATCCTAG TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813083.1
    CDS7..6084
    Translation

    Target ORF information:

    RefSeq Version XM_010814781.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCA GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGTGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATATGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGCGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGATCCTAG TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216201
    Clone ID Related Accession (Same CDS sequence) NM_001192910.2
    Accession Version NM_001192910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02047080.1, DAAA02047081.1 and DAAA02047082.1. On Jun 30, 2017 this sequence version replaced NM_001192910.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCGGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG CGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA CGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGA GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179839.1
    CDS1..7695
    Translation

    Target ORF information:

    RefSeq Version NM_001192910.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCGGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG CGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA CGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGA GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38868
    Clone ID Related Accession (Same CDS sequence) NM_001192910.1
    Accession Version NM_001192910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product spectrin beta chain, non-erythrocytic 4
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03038929.1, AAFC03038928.1, AAFC03032912.1 and AAFC03038927.1. On Jul 17, 2010 this sequence version replaced XM_617751.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCGGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG TGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA CGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGA GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179839.1
    CDS1..7695
    Translation

    Target ORF information:

    RefSeq Version NM_001192910.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus spectrin, beta, non-erythrocytic 4 (SPTBN4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGGC TACCAGCCCC CAATAATAAC 
    AACCTTTCAG CCCGCTGGGA CAGTCCTGAT CGGGGCTGGG AGCGGGAGCA ACCAGCCGCC
    TCCACTGCAG CAGCCTCACT CTTTGAGTGC TCCCGGATCA AGGCCTTGGC AGATGAGCGG
    GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAACT CGCACCTCGC CCGCGTCGGC
    TGCCACATTG GGGACCTCTA CACAGACCTC AGGGACGGCT TCGTGCTCAC GCGGCTGCTG
    GAAGTGCTGT CCGGGGAGCA GCTGCCAAGG CCCACGCGTG GTCGCATGAG GATCCACTCA
    CTGGAAAACG TAGACAAGGC CCTGCAGTTC CTGAAGGAGC AGCGTGTGCA CCTGGAGAAC
    GTGGGCTCAC ATGACATCGT GGACGGGAAC CACCGACTGA CCTTGGGGCT GGTCTGGACC
    ATCATCCTTC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CTGAGGACAA CAGAGAGACA
    CGCTCAGCCA AGGACGCGCT GCTCTTGTGG TGTCAGATGA AGACAGCTGG TTACCCCGAG
    GTAAACATCC AGAATTTCAC CACCAGCTGG CGTGACGGCT TGGCCTTCAA TGCCCTCATT
    CACCGGCACA GGCCCGATCT CGTGGACTTC AGCAAGCTCA CCAAGTCCAA CGCCAACTAC
    AATCTGCAGA GAGCCTTCCG CACGGCTGAG CAGCACTTGG GGCTGGCACG TCTCCTGGAC
    CCTGAGGATG TGAACATGGA GGCTCCCGAT GAGAAGTCCA TCATCACCTA TGTGGTCTCT
    TTCTACCATT ATTTCTCCAA GATGAAGGCT CTGGCTGTGG AGGGGAAACG GATTGGAAAG
    GTCCTGGACC AGGTGCTGGA GGTGGGGAAG ATCATTGAGC GCTACGAGGA GCTGGCAACC
    GAGCTGCTGG CCTGGATCCA CCGCACCGTG GGCCTCATCA GCAACCAGAA GTTTGCCAAC
    TCATTAAGTG GGGTGCAGCA GCAGCTTCAG GCTTTCACGG CCTACTGCAC GCTGGAGAAG
    CCTGTCAAGT TTCAGGAGAA GGGAAACCTG GAGGTGCTGC TCTTCAGCAT CCAGAGCAAA
    CTGCGGGCCT GCAACCGTCG TCTCTACGTG CCCCGGGAGG GCTGTGGCAT CTGGGATATT
    GACAAGGCAT GGGGACAACT GGAGAAGGCA GAACATGAGC GAGAGGCTGC CCTCCGGGCC
    GAGCTGATCC GGCAGGAGAA GCTGGAACTG CTGGCCCAGA GGTTTGACCA CAAGGTGGCC
    ATGCGGGAGA GCTGGCTGAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA TTTTGGATAT
    GAGCTGCCAG CGGTGGAGGC AGCCATGAAG AAACACGAAG CCATCGAGGC AGACATAGCA
    GCCTATGAGG AGCGGGTGCA GGGCGTGGCA GAGCTGGCCC AGGCCTTGGC AACTGAAGGT
    TACTATGATG CCCGCCGGGT GGCAGCCCAG CGTGACAGTG TCCTGCGCCA GTGGGCCCTG
    CTGACCGGGC TGGTAGGTGC CCGGCGGACA CGGCTCGAGC AGAACCTGGC TCTGCAGAAG
    GTCTTCCAGG AGATGGTGTA CATGGTGGAC TGGATGGAGG AGATGCAGGC TCAGCTGCTG
    TCCCGGGAGT GTGGGCAGCA CCTGGTGGAG GCGGAGGACC TGTTGCAGAA GCATGGACTG
    CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGGGTGGAGG CCCTCAATGC TGCTGCCCTG
    CGTTTCTCAC AGCTGCAGGG TTACCAGCCC TGCGATCCGC AGGTCATCTG CAACCGCGTG
    AACCACGTGC ACGGCTGTCT GGCGGAGCTG CAGGAGCAGG CGGCCAGGCG GCGCGCGGAG
    CTGGAGGCCT CGCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAAGC CGAGAGCTGG
    GCCCGCGACA AGGAGCGCCT CCTGGAGGCG GCGGGCGGCG GCGGCGCGGC GGGCGCAACG
    GGCGCGGCGG GCGGCGCACA CGACCTGTCC AGCACCGCGC GCCTCCTGGC GCAGCACAAG
    ATCCTGCAGG GCGAGCTGGG CGGGCGGCGG GCGCTGCTGC AGCAGGCACT GCGGCGGGGC
    GAGGAGCTGG CCGCGGCGGG TGGCGCCGTG GGCCCGGGCG CCGAGACGGT GCACCTGGCG
    GGCTTGGCGG AGCGCGCGGC GAGCGCGCGG CGGCGCTGGC AGCGGCTGGA GGAGGCGGCG
    GCAAGGCGCG AGCGGCGGCT GCAGGAGGCG CGGGCGCTGC ACCAGTTCGG AGCGGACCTC
    GACGGGCTCC TGGACTGGCT TCGCGACGCC TACCGCCTGG CAGCCGCGGG CGACTTCGGC
    CACGACGAAG CGTCCAGCCG CCGCCTGGCC CGCCAGCACC GCGCGCTCAC AGGGGAGGTG
    GAGGCGCATC GCGGGCCCGT GGGCGGCCTG CGGCGTCAGC TGGCGACACT GGGGGGAGCC
    AGCGGAGCCG GGCCCCTGGT GGTGGCACTG CAGGTGCGCG TGGTGGAGGC CGAGCAGCTG
    TTCGCAGAGG TGACCGAGGT GGCTGCGCTG CGGCGCCAGT GGCTGCGCGA TGCCCTCGCT
    GTCTACCGCA TGTTCGGCGA GGTGCACGCG TGCGAGCTGT GGATTGGGGA GAAGGAGCAG
    TGGCTGCTGG CCATGCGCGT GCCCAATTCA TTGGACGACG TCGAGGTGGT GCAGCACCGA
    TTTGAGAGTC TGGACCAGGA GATGAACAGT CTGATGGGCC GTGTCCTGGA TGTGAACCAC
    ACGGTTCAGG AACTGGTGGA AGGAGGTCAC CCCAGCTCAG ATGAGGTGCG TTCCTGCCAG
    GACCACCTCA ACAGCAGGTG GAACCGCATC GTGGAGCTGG TGGAACAGCG AAAAGAAGAG
    GTGAGCGCGG TGCTGCTAGT GGAGAACCAC GTGCTGGAGG TGGCGGAGGT GCGCACCCAG
    GTGCGCGAGA AGCGGAGGGC TGTGGAGAGC GCGCCCCGGG CGGGCGGCGC CCTGCAGTGG
    CGCCTTAGCG GCCTGGAGGC GGCTCTGCAA GCGCTAGAGC CGCGCCAGGC GGCCCTCCTG
    GAGGAGGCGG CCCTGCTGGC TGTGCGCTTC CCGGCTCAGG CTGCGCGGCT GCATCAGGGT
    GCGGAGGAAC TGGGCGCCGA GTGGGGCGCT CTGGCCAGCG CGGCTCAGGC CTGTGGCGAG
    GCGGTGGCGG CCGCCGGACG CCTGCAGCGC TTTCTGCACG ACCTAGACGC CTTCCTGGAC
    TGGCTGGTAC GCGCCCAAGA GACGGCGGGC GGCGGCGAGG GGCCCCTGCC CCGCAGCCTG
    GAAGAGGCAG ACGCGCTGCT GGCGCGCCAC GCGGCGCTCA AGGAGGAAGT GGACCAGCGA
    GAGGAGGACT ACGCGCGCAT CGTGGCGGCC AGCGAGGCGC TGCTGGCGGG CGACGGCGCG
    GAGCTGGGCC CGGGCCTGGC ACTCGACGAG TGGCTGCCAC ACCTCGAACT TGGTTGGCAC
    AAGCTGCTCG GCTTGTGGGA GGCGCGCAGG GAGGCCCTGG TCCAGGCGCA CGTCTACCAG
    CTCTTTCTGC GCGACCTACG CCAGGCGCGA GCGGTGCTAC GCAACCAGGA GATGGCACTG
    TCAGGTCAGG AGCTGCCGGG CACCGTGGAG TCGGTGGAGG AGGCGCTGAA GCGGCACCGC
    GATTTCCTCA CCACGATGGA GCTGAACCAG CAGAAGATGC AGCTGGCCGT GCAGGCTGCC
    GAGGGCCTGC TGAGACAGGG CAACGCCTAC GGGGAGCAGG CCCAGGAGGC TGTGACGCGG
    CTGCTGGAGA AGAGCCAAGA AAATCAACTA CGGGCCCATC AATGGATGCA GAAGCTACAC
    GACCAGCTTG GGCTGCAGCA CTTCCTCCGA GACTGCCACG AGCTGGATGG CTGGATCCAT
    GAGAAGATGC TGATGGCGCG GGACGGCACA CGGGAAGACG GCCACAAGCT GCATAAGAGA
    TGGCTCCGGC ACCAGGCATT CATGGCCGAG CTGGCTCAGA ATAAGGAGTG GCTGGAGAAG
    ATTGAGAGGG AGGGCCAGCA ACTGATGCAG GAGAAACCAG AGCTGGCGGC CTCAGTGCGG
    AAGAAGCTGG GCGAGATCCG GCAGTGCTGG GCGGAGCTGG AAAGCACCAC TCAGGCCAAG
    GCGAGGCAGC TCTTTGAGGC CAGTAAGGCT GACCAACTGG TGCAGAGCTT CGCGGAGCTG
    GACAAGAAAC TCCTTCACAT GGAAAGCCAG CTGCAAGACG TGGATCCTGG TGGGGACCTG
    GCCACTGTCA ACAGCCAGCT GAAGAAGCTG CAGTCGATGG AGTCGCAGGT GGAGGAGTGG
    TACCGCGAGG TGGGGGAGTT GCAGGCGCAG ACGGCAGCGC TGCCCCTGGA GCCAGCGAGC
    AAGGAGCTGG TGGGCGAGCG GCAGAACGCG GTGGGCGAGC GCCTGGTGCG CCTGCTCGAG
    CCGCTGCAGG AGCGCCGCCG CCTGCTGCTC GCCTCCAAGG AGCTGCACCA GGTGGCGCAC
    GACCTCGAGG ACGAGCTGGC ATGGGTCCAG GAGCGACTGC CACTGGCCAT GCAGACAGAG
    CGAGGCAGTG GTTTGCAGGC CGTCCAGCAG TACATCAAAA AGAACCAGGG CCTGCGGCGG
    GAGATCCAGG CGCACGGGCC GCGCCTGGAG GAGGTGCTGG AGCGCGCGGG TGCCCTGGCG
    TCGCTGCGCA GCCCCGAGGC TGAGGCCGTG CGCCGCGGCC AGGAGCAGCT GCAGGGCGCC
    TGGGCCGGGC TGCGGGAGGC GGCCGAGCGG CGGCAGCAGG TGCTCGACGC CGCCTTCCAG
    GTGGAACAGT ACTACTTCGA CGTGGCCGAG GTGGAGGCGT GGCTGGGCGA GCAGGAGCTG
    CTCATGATGA GTGAGGACAA GGGCAAGGAC GAACAGAGCA CCCTGCAGCT GCTCAAGAAG
    CACCTGCAGC TGGAGCAGGG CGTCGAGAAC TACGAGGAAA GCATCGCGCA GCTGTCGCGC
    CAGTGCCGGG CGCTGCTGGA GATGGGGCAC CCGGACAGCG AGCAGATCAG CCGGCGGCAG
    TCTCAGGTGG ACCGCCTATA CGTGGCGCTC AAGGAGCTGG GCGAGGAACG CCGGGTGGGC
    CTGGAGCAGC AGTACTGGCT GTACCAGCTC AGTCGCCAGG TGGACGAGCT CGAGCACTGG
    ATCGCAGAGA AGGAGGTGGT GGCCGGCTCT CCTGAGCTCG GCCAGGACTT TGAGCATGTC
    ACGGTGCTAC AGGAGAAATT CTCAGAATTC GCCAGCGAGA CTGGCACGGC AGGGCGGGAG
    CGGCTGGCGG CCGTGAACCA GATGGTGGAT GAGCTGATCG AGTGTGGCCA CACGGCGGCG
    GCCACCATGG CCGAGTGGAA GGACGGGCTG AATGAGGCCT GGGCTGAGCT GCTGGAGCTT
    ATGGGCACGA GGGCCCAACT GCTGGCTGCC TCTCGGGAGC TGCATAAGTT CTTCAGTGAT
    GCCCGAGAAC TCCAAGGACA GATTGAGGAG AAGCGGAGGC GACTGCCTCG CCTGACCACC
    CCGCCAGAGC CGAGACCCAG TGCCAGCTCC ATGCAGCGGA CCCTGCGCGC CTTTGAACAT
    GACCTGCAGC TCCTTGTGTC CCAGGTACGG CAGCTGCAGG AGGGGGCAGC CCAGCTGCGG
    ACGGTGTACG CGGGCGAGCA CGCGGAGGCC ATCGCCAGCC GGGAGCAGGA GGTGCTGCAA
    GGCTGGAAGG AGCTGCTGGC AGCCTGCGAG GACGCCCGCC TGCACGTGAG CTCCACGGCC
    GACGCCCTGC GATTCCACAA CCAGGCCCGA GACCTGCTCT CCTGGATGGA TGGCATCGCT
    GGCCAGATTG GGGCAGCTGA CAAGCCCAGG GACGTGTCAT CGGTGGAGGT GCTCATGAAC
    TACCACCAGG GCCTGAAGAC TGAACTGGAG GCACGGATGC CAGAGCTGAC AGCCTGCCAG
    GAGCTGGGGC GCTCTCTGCT GCTCAACAAG AGTGCCATGG CTGACGAGAT CCAGGCACAG
    CTGGACAAGC TGGGGACCAG GAAGGAGGAA GTGTCGGATA AGTGGGACCG CCACTGGGAG
    TGGCTGCAGC AGATGCTGGA AGTGCACCAG TTTGCCCAAG AGGCAGTGGT GGCCGATGCC
    TGGCTGACAG CCCAGGAGCC GCTTTTGCAG AGCCGGGAGC TGGGCAGCAG TGTGGATGAA
    GTGGAACAGC TTATCCGGCG GCACGAGGCC TTCCGCAAAG CGGCCGCAGC CTGGGAAGAA
    AGGTTCAGCT CTCTGCGGCG CCTGACCACG ATTGAGAAAC TCAAGGCAGA ACAGAGCAAG
    CAGCCGCCCA CCCCCCTCCT GGGGCGCAAG TTCTTTGGAG ACCCTACGGA ACTGGCGGCC
    AAGGCCGCGC CCCTGCTGCG GCCAGGGGGC TCTGAGAGGG GCTTGGAGCC GCTGGCCCGC
    CGGGCCTCAG ACACGCTCTC GGCTGAGGTG CGGACCCGGG TGGGGTACGT GCGGCAGGAG
    CTCAAGCCGG AGCGCCTCCA GCCGCGCATC GACCGGCTGC CCGAGGTCCC GGGGAGGGTG
    GAGCCTACGG CCCAGCCGGC GGCACCAGAG GACGCCGCTG AGACCCCCGG GGCCCCTGCG
    GTAGTCGCAG CGGAGCAGGT CCGGCCGCGG CCCGAGCGGC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGAG GCGGCCCGAG CGGCAGGAAT CAGCCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC AGAGCGGCAG GAGTCAACGG AGCACGAGGT GGCACACAGC
    CTTACCCTGG GCCGCTACGA GCAGATGGAG AGGCGGCGGG AGCGGCGGGA GCGGCGGTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGC CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAGCAACTG CAGGAGAAAG AGGCAGGCCC AGGGCTCCCT
    GTTGGGCCTT CGCTGCCTCA GCCTCGCGAA CTTCCCCCCG GCCGCCTGCC CAACGGGCTT
    GAGCCGCCCG AGCGGACACC TCGGCCGGAC CGGCCGCGGG CTCGGGACCG GCCCAAGCCG
    AGACGGCGGC CGCGGCCCCG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCACCAC CGCCCACTCA TACAGTGCAG
    CACGAGGGCT TCCTGCTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCATGGGTGA GCCTGTACTG CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC
    AAGGGTCCGG CATCCGGGGG CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GACGGCAGTG AGTTTTTGCT CCAGGCTAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTGGCCG CCTCGGTTGG GGAACACGCC GAGATCGCCC GCTGGGGCCA GACACAACCC
    ACCACATCGT CCACAGACGA GGGCAACCCC AAGCGGGAGG CAGGGGAGCG CAGGGCCAGC
    GGGCGCCGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28665
    Clone ID Related Accession (Same CDS sequence) XM_005218990.3
    Accession Version XM_005218990.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGC CTTCGCTGCC TCAGCCTCGC
    GAACTTCCCC CCGGCCGCCT GCCCAACGGG CTTGAGCCGC CCGAGCGGAC ACCTCGGCCG
    GACCGGCCGC GGGCTCGGGA CCGGCCCAAG CCGAGACGGC GGCCGCGGCC CCGAGAGGGT
    GGTGAGGGCG GGGGAAGCCG GCGCTCGCGC TCCGCCCCGG CCCAGGGCGG CTCCGCCCCC
    GCGCCTCCAC CACCGCCCAC TCATACAGTG CAGCACGAGG GCTTCCTGCT GCGCAAGCGC
    GAGCTCGACG CTAACCGCAA GTCGTCCAAC CGGTCATGGG TGAGCCTGTA CTGCGTGCTC
    AGCAAGGGGG AGCTGGGTTT CTACAAGGAC GCCAAGGGTC CGGCATCCGG GGGCACACAC
    GGCGGGGAGC CGCTGCTCAG CCTGCACAAG GCCACCAGCG AGGTGGCTAG TGACTACAAG
    AAAAAGAAGC ATGTCTTCAA GCTCCAGACC CAGGACGGCA GTGAGTTTTT GCTCCAGGCT
    AAAGATGAGG AGGAGATGAA CGGCTGGCTG GAGGCTGTGG CCGCCTCGGT TGGGGAACAC
    GCCGAGATCG CCCGCTGGGG CCAGACACAA CCCACCACAT CGTCCACAGA CGAGGGCAAC
    CCCAAGCGGG AGGCAGGGGA GCGCAGGGCC AGCGGGCGCC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219047.1
    CDS5..7852
    Translation

    Target ORF information:

    RefSeq Version XM_005218990.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218990.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGC CTTCGCTGCC TCAGCCTCGC
    GAACTTCCCC CCGGCCGCCT GCCCAACGGG CTTGAGCCGC CCGAGCGGAC ACCTCGGCCG
    GACCGGCCGC GGGCTCGGGA CCGGCCCAAG CCGAGACGGC GGCCGCGGCC CCGAGAGGGT
    GGTGAGGGCG GGGGAAGCCG GCGCTCGCGC TCCGCCCCGG CCCAGGGCGG CTCCGCCCCC
    GCGCCTCCAC CACCGCCCAC TCATACAGTG CAGCACGAGG GCTTCCTGCT GCGCAAGCGC
    GAGCTCGACG CTAACCGCAA GTCGTCCAAC CGGTCATGGG TGAGCCTGTA CTGCGTGCTC
    AGCAAGGGGG AGCTGGGTTT CTACAAGGAC GCCAAGGGTC CGGCATCCGG GGGCACACAC
    GGCGGGGAGC CGCTGCTCAG CCTGCACAAG GCCACCAGCG AGGTGGCTAG TGACTACAAG
    AAAAAGAAGC ATGTCTTCAA GCTCCAGACC CAGGACGGCA GTGAGTTTTT GCTCCAGGCT
    AAAGATGAGG AGGAGATGAA CGGCTGGCTG GAGGCTGTGG CCGCCTCGGT TGGGGAACAC
    GCCGAGATCG CCCGCTGGGG CCAGACACAA CCCACCACAT CGTCCACAGA CGAGGGCAAC
    CCCAAGCGGG AGGCAGGGGA GCGCAGGGCC AGCGGGCGCC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53693
    Clone ID Related Accession (Same CDS sequence) XM_010814780.2
    Accession Version XM_010814780.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGG TCATGGGTGA GCCTGTACTG
    CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC AAGGGTCCGG CATCCGGGGG
    CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC ACCAGCGAGG TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813082.1
    CDS7..7323
    Translation

    Target ORF information:

    RefSeq Version XM_010814780.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGGACGTGT CATCGGTGGA GGTGCTCATG AACTACCACC AGGGCCTGAA GACTGAACTG
    GAGGCACGGA TGCCAGAGCT GACAGCCTGC CAGGAGCTGG GGCGCTCTCT GCTGCTCAAC
    AAGAGTGCCA TGGCTGACGA GATCCAGGCA CAGCTGGACA AGCTGGGGAC CAGGAAGGAG
    GAAGTGTCGG ATAAGTGGGA CCGCCACTGG GAGTGGCTGC AGCAGATGCT GGAAGTGCAC
    CAGTTTGCCC AAGAGGCAGT GGTGGCCGAT GCCTGGCTGA CAGCCCAGGA GCCGCTTTTG
    CAGAGCCGGG AGCTGGGCAG CAGTGTGGAT GAAGTGGAAC AGCTTATCCG GCGGCACGAG
    GCCTTCCGCA AAGCGGCCGC AGCCTGGGAA GAAAGGTTCA GCTCTCTGCG GCGCCTGACC
    ACGATTGAGA AACTCAAGGC AGAACAGAGC AAGCAGCCGC CCACCCCCCT CCTGGGGCGC
    AAGTTCTTTG GAGACCCTAC GGAACTGGCG GCCAAGGCCG CGCCCCTGCT GCGGCCAGGG
    GGCTCTGAGA GGGGCTTGGA GCCGCTGGCC CGCCGGGCCT CAGACACGCT CTCGGCTGAG
    GTGCGGACCC GGGTGGGGTA CGTGCGGCAG GAGCTCAAGC CGGAGCGCCT CCAGCCGCGC
    ATCGACCGGC TGCCCGAGGT CCCGGGGAGG GTGGAGCCTA CGGCCCAGCC GGCGGCACCA
    GAGGACGCCG CTGAGACCCC CGGGGCCCCT GCGGTAGTCG CAGCGGAGCA GGTCCGGCCG
    CGGCCCGAGC GGCAGGAGTC AGCTGATCGC GCGGAGGAGC TGCCCAGGAG GAGGCGGCCC
    GAGCGGCAGG AATCAGCCGA TCAATCCGAG GAGGCTGCGC GGAGGCGGCG GCCAGAGCGG
    CAGGAGTCAA CGGAGCACGA GGTGGCACAC AGCCTTACCC TGGGCCGCTA CGAGCAGATG
    GAGAGGCGGC GGGAGCGGCG GGAGCGGCGG TTGGAGCGGC AGGAGTCCAG CGAACAGGAG
    ATGCCCATCA GAGGAGACCT GGCCAAGGGG AAGGCCACCC TGGCTGACAT TGTGGAGCAA
    CTGCAGGAGA AAGAGGCAGG CCCAGGGCTC CCTGTTGGGG TCATGGGTGA GCCTGTACTG
    CGTGCTCAGC AAGGGGGAGC TGGGTTTCTA CAAGGACGCC AAGGGTCCGG CATCCGGGGG
    CACACACGGC GGGGAGCCGC TGCTCAGCCT GCACAAGGCC ACCAGCGAGG TGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53694
    Clone ID Related Accession (Same CDS sequence) XM_010814781.2
    Accession Version XM_010814781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGATCCTAG TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813083.1
    CDS11..6088
    Translation

    Target ORF information:

    RefSeq Version XM_010814781.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCGGATCT GGCCGAGCGG CGGAGCCGGT GGGGCCGAGC CGAGCCGGCT GGGCCGGGCC 
    GGGCCGAGGA CACCGACAGG GAGGGCGGTC GGCCACGGCG AGCAGCGGGG ACAGCGGCAC
    CGGCGGCCGC GGGGCCAGGC CTCATCTCCC CCAATGGCGC AGGTACCAGG GGAAGTGGAC
    AACATGGAGG GGCTACCAGC CCCCAATAAT AACAACCTTT CAGCCCGCTG GGACAGTCCT
    GATCGGGGCT GGGAGCGGGA GCAACCAGCC GCCTCCACTG CAGCAGCCTC ACTCTTTGAG
    TGCTCCCGGA TCAAGGCCTT GGCAGATGAG CGGGAGGCTG TGCAGAAGAA AACCTTCACC
    AAGTGGGTGA ACTCGCACCT CGCCCGCGTC GGCTGCCACA TTGGGGACCT CTACACAGAC
    CTCAGGGACG GCTTCGTGCT CACGCGGCTG CTGGAAGTGC TGTCCGGGGA GCAGCTGCCA
    AGGCCCACGC GTGGTCGCAT GAGGATCCAC TCACTGGAAA ACGTAGACAA GGCCCTGCAG
    TTCCTGAAGG AGCAGCGTGT GCACCTGGAG AACGTGGGCT CACATGACAT CGTGGACGGG
    AACCACCGAC TGACCTTGGG GCTGGTCTGG ACCATCATCC TTCGCTTCCA GATTCAAGTC
    ATCAAGATTG AGACTGAGGA CAACAGAGAG ACACGCTCAG CCAAGGACGC GCTGCTCTTG
    TGGTGTCAGA TGAAGACAGC TGGTTACCCC GAGGTAAACA TCCAGAATTT CACCACCAGC
    TGGCGTGACG GCTTGGCCTT CAATGCCCTC ATTCACCGGC ACAGGCCCGA TCTCGTGGAC
    TTCAGCAAGC TCACCAAGTC CAACGCCAAC TACAATCTGC AGAGAGCCTT CCGCACGGCT
    GAGCAGCACT TGGGGCTGGC ACGTCTCCTG GACCCTGAGG ATGTGAACAT GGAGGCTCCC
    GATGAGAAGT CCATCATCAC CTATGTGGTC TCTTTCTACC ATTATTTCTC CAAGATGAAG
    GCTCTGGCTG TGGAGGGGAA ACGGATTGGA AAGGTCCTGG ACCAGGTGCT GGAGGTGGGG
    AAGATCATTG AGCGCTACGA GGAGCTGGCA ACCGAGCTGC TGGCCTGGAT CCACCGCACC
    GTGGGCCTCA TCAGCAACCA GAAGTTTGCC AACTCATTAA GTGGGGTGCA GCAGCAGCTT
    CAGGCTTTCA CGGCCTACTG CACGCTGGAG AAGCCTGTCA AGTTTCAGGA GAAGGGAAAC
    CTGGAGGTGC TGCTCTTCAG CATCCAGAGC AAACTGCGGG CCTGCAACCG TCGTCTCTAC
    GTGCCCCGGG AGGGCTGTGG CATCTGGGAT ATTGACAAGG CATGGGGACA ACTGGAGAAG
    GCAGAACATG AGCGAGAGGC TGCCCTCCGG GCCGAGCTGA TCCGGCAGGA GAAGCTGGAA
    CTGCTGGCCC AGAGGTTTGA CCACAAGGTG GCCATGCGGG AGAGCTGGCT GAATGAGAAC
    CAGCGCCTGG TCTCCCAGGA CAATTTTGGA TATGAGCTGC CAGCGGTGGA GGCAGCCATG
    AAGAAACACG AAGCCATCGA GGCAGACATA GCAGCCTATG AGGAGCGGGT GCAGGGCGTG
    GCAGAGCTGG CCCAGGCCTT GGCAACTGAA GGTTACTATG ATGCCCGCCG GGTGGCAGCC
    CAGCGTGACA GTGTCCTGCG CCAGTGGGCC CTGCTGACCG GGCTGGTAGG TGCCCGGCGG
    ACACGGCTCG AGCAGAACCT GGCTCTGCAG AAGGTCTTCC AGGAGATGGT GTACATGGTG
    GACTGGATGG AGGAGATGCA GGCTCAGCTG CTGTCCCGGG AGTGTGGGCA GCACCTGGTG
    GAGGCGGAGG ACCTGTTGCA GAAGCATGGA CTGCTGGAGG GGGACATCGC AGCCCAGAGT
    GAGCGGGTGG AGGCCCTCAA TGCTGCTGCC CTGCGTTTCT CACAGCTGCA GGGTTACCAG
    CCCTGCGATC CGCAGGTCAT CTGCAACCGC GTGAACCACG TGCACGGCTG TCTGGCGGAG
    CTGCAGGAGC AGGCGGCCAG GCGGCGCGCG GAGCTGGAGG CCTCGCGGAG CCTGTGGGCG
    CTGCTGCAGG AGCTGGAGGA AGCCGAGAGC TGGGCCCGCG ACAAGGAGCG CCTCCTGGAG
    GCGGCGGGCG GCGGCGGCGC GGCGGGCGCA ACGGGCGCGG CGGGCGGCGC ACACGACCTG
    TCCAGCACCG CGCGCCTCCT GGCGCAGCAC AAGATCCTGC AGGGCGAGCT GGGCGGGCGG
    CGGGCGCTGC TGCAGCAGGC ACTGCGGCGG GGCGAGGAGC TGGCCGCGGC GGGTGGCGCC
    GTGGGCCCGG GCGCCGAGAC GGTGCACCTG GCGGGCTTGG CGGAGCGCGC GGCGAGCGCG
    CGGCGGCGCT GGCAGCGGCT GGAGGAGGCG GCGGCAAGGC GCGAGCGGCG GCTGCAGGAG
    GCGCGGGCGC TGCACCAGTT CGGAGCGGAC CTCGACGGGC TCCTGGACTG GCTTCGCGAC
    GCCTACCGCC TGGCAGCCGC GGGCGACTTC GGCCACGACG AAGCGTCCAG CCGCCGCCTG
    GCCCGCCAGC ACCGCGCGCT CACAGGGGAG GTGGAGGCGC ATCGCGGGCC CGTGGGCGGC
    CTGCGGCGTC AGCTGGCGAC ACTGGGGGGA GCCAGCGGAG CCGGGCCCCT GGTGGTGGCA
    CTGCAGGTGC GCGTGGTGGA GGCCGAGCAG CTGTTCGCAG AGGTGACCGA GGTGGCTGCG
    CTGCGGCGCC AGTGGCTGCG CGATGCCCTC GCTGTCTACC GCATGTTCGG CGAGGTGCAC
    GCGTGCGAGC TGTGGATTGG GGAGAAGGAG CAGTGGCTGC TGGCCATGCG CGTGCCCAAT
    TCATTGGACG ACGTCGAGGT GGTGCAGCAC CGATTTGAGA GTCTGGACCA GGAGATGAAC
    AGTCTGATGG GCCGTGTCCT GGATGTGAAC CACACGGTTC AGGAACTGGT GGAAGGAGGT
    CACCCCAGCT CAGATGAGGT GCGTTCCTGC CAGGACCACC TCAACAGCAG GTGGAACCGC
    ATCGTGGAGC TGGTGGAACA GCGAAAAGAA GAGGTGAGCG CGGTGCTGCT AGTGGAGAAC
    CACGTGCTGG AGGTGGCGGA GGTGCGCACC CAGGTGCGCG AGAAGCGGAG GGCTGTGGAG
    AGCGCGCCCC GGGCGGGCGG CGCCCTGCAG TGGCGCCTTA GCGGCCTGGA GGCGGCTCTG
    CAAGCGCTAG AGCCGCGCCA GGCGGCCCTC CTGGAGGAGG CGGCCCTGCT GGCTGTGCGC
    TTCCCGGCTC AGGCTGCGCG GCTGCATCAG GGTGCGGAGG AACTGGGCGC CGAGTGGGGC
    GCTCTGGCCA GCGCGGCTCA GGCCTGTGGC GAGGCGGTGG CGGCCGCCGG ACGCCTGCAG
    CGCTTTCTGC ACGACCTAGA CGCCTTCCTG GACTGGCTGG TACGCGCCCA AGAGACGGCG
    GGCGGCGGCG AGGGGCCCCT GCCCCGCAGC CTGGAAGAGG CAGACGCGCT GCTGGCGCGC
    CACGCGGCGC TCAAGGAGGA AGTGGACCAG CGAGAGGAGG ACTACGCGCG CATCGTGGCG
    GCCAGCGAGG CGCTGCTGGC GGGCGACGGC GCGGAGCTGG GCCCGGGCCT GGCACTCGAC
    GAGTGGCTGC CACACCTCGA ACTTGGTTGG CACAAGCTGC TCGGCTTGTG GGAGGCGCGC
    AGGGAGGCCC TGGTCCAGGC GCACGTCTAC CAGCTCTTTC TGCGCGACCT ACGCCAGGCG
    CGAGCGGTGC TACGCAACCA GGAGATGGCA CTGTCAGGTC AGGAGCTGCC GGGCACCGTG
    GAGTCGGTGG AGGAGGCGCT GAAGCGGCAC CGCGATTTCC TCACCACGAT GGAGCTGAAC
    CAGCAGAAGA TGCAGCTGGC CGTGCAGGCT GCCGAGGGCC TGCTGAGACA GGGCAACGCC
    TACGGGGAGC AGGCCCAGGA GGCTGTGACG CGGCTGCTGG AGAAGAGCCA AGAAAATCAA
    CTACGGGCCC ATCAATGGAT GCAGAAGCTA CACGACCAGC TTGGGCTGCA GCACTTCCTC
    CGAGACTGCC ACGAGCTGGA TGGCTGGATC CATGAGAAGA TGCTGATGGC GCGGGACGGC
    ACACGGGAAG ACGGCCACAA GCTGCATAAG AGATGGCTCC GGCACCAGGC ATTCATGGCC
    GAGCTGGCTC AGAATAAGGA GTGGCTGGAG AAGATTGAGA GGGAGGGCCA GCAACTGATG
    CAGGAGAAAC CAGAGCTGGC GGCCTCAGTG CGGAAGAAGC TGGGCGAGAT CCGGCAGTGC
    TGGGCGGAGC TGGAAAGCAC CACTCAGGCC AAGGCGAGGC AGCTCTTTGA GGCCAGTAAG
    GCTGACCAAC TGGTGCAGAG CTTCGCGGAG CTGGACAAGA AACTCCTTCA CATGGAAAGC
    CAGCTGCAAG ACGTGGATCC TGGTGGGGAC CTGGCCACTG TCAACAGCCA GCTGAAGAAG
    CTGCAGTCGA TGGAGTCGCA GGTGGAGGAG TGGTACCGCG AGGTGGGGGA GTTGCAGGCG
    CAGACGGCAG CGCTGCCCCT GGAGCCAGCG AGCAAGGAGC TGGTGGGCGA GCGGCAGAAC
    GCGGTGGGCG AGCGCCTGGT GCGCCTGCTC GAGCCGCTGC AGGAGCGCCG CCGCCTGCTG
    CTCGCCTCCA AGGAGCTGCA CCAGGTGGCG CACGACCTCG AGGACGAGCT GGCATGGGTC
    CAGGAGCGAC TGCCACTGGC CATGCAGACA GAGCGAGGCA GTGGTTTGCA GGCCGTCCAG
    CAGTACATCA AAAAGAACCA GGGCCTGCGG CGGGAGATCC AGGCGCACGG GCCGCGCCTG
    GAGGAGGTGC TGGAGCGCGC GGGCGCCCTG GCGTCGCTGC GCAGCCCCGA GGCTGAGGCC
    GTGCGCCGCG GCCAGGAGCA GCTGCAGGGC GCCTGGGCCG GGCTGCGGGA GGCGGCCGAG
    CGGCGGCAGC AGGTGCTCGA CGCCGCCTTC CAGGTGGAAC AGTACTACTT CGACGTGGCC
    GAGGTGGAGG CGTGGCTGGG CGAGCAGGAG CTGCTCATGA TGAGTGAGGA CAAGGGCAAG
    GACGAACAGA GCACCCTGCA GCTGCTCAAG AAGCACCTGC AGCTGGAGCA GGGCGTCGAG
    AACTACGAGG AAAGCATCGC GCAGCTGTCG CGCCAGTGCC GGGCGCTGCT GGAGATGGGG
    CACCCGGACA GCGAGCAGAT CAGCCGGCGG CAGTCTCAGG TGGACCGCCT ATACGTGGCG
    CTCAAGGAGC TGGGCGAGGA ACGCCGGGTG GGCCTGGAGC AGCAGTACTG GCTGTACCAG
    CTCAGTCGCC AGGTGGACGA GCTCGAGCAC TGGATCGCAG AGAAGGAGGT GGTGGCCGGC
    TCTCCTGAGC TCGGCCAGGA CTTTGAGCAT GTCACGGTGC TACAGGAGAA ATTCTCAGAA
    TTCGCCAGCG AGACTGGCAC GGCAGGGCGG GAGCGGCTGG CGGCCGTGAA CCAGATGGTG
    GATGAGCTGA TCGAGTGTGG CCACACGGCG GCGGCCACCA TGGCCGAGTG GAAGGACGGG
    CTGAATGAGG CCTGGGCTGA GCTGCTGGAG CTTATGGGCA CGAGGGCCCA ACTGCTGGCT
    GCCTCTCGGG AGCTGCATAA GTTCTTCAGT GATGCCCGAG AACTCCAAGG ACAGATTGAG
    GAGAAGCGGA GGCGACTGCC TCGCCTGACC ACCCCGCCAG AGCCGAGACC CAGTGCCAGC
    TCCATGCAGC GGACCCTGCG CGCCTTTGAA CATGACCTGC AGCTCCTTGT GTCCCAGGTA
    CGGCAGCTGC AGGAGGGGGC AGCCCAGCTG CGGACGGTGT ACGCGGGCGA GCACGCGGAG
    GCCATCGCCA GCCGGGAGCA GGAGGTGCTG CAAGGCTGGA AGGAGCTGCT GGCAGCCTGC
    GAGGACGCCC GCCTGCACGT GAGCTCCACG GCCGACGCCC TGCGATTCCA CAACCAGGCC
    CGAGACCTGC TCTCCTGGAT GGATGGCATC GCTGGCCAGA TTGGGGCAGC TGACAAGCCC
    AGGATCCTAG TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53695
    Clone ID Related Accession (Same CDS sequence) XM_010814783.2 , XM_010814782.1
    Accession Version XM_010814783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATACGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGAGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813085.1
    CDS131..3859
    Translation

    Target ORF information:

    RefSeq Version XM_010814783.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATACGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGAGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53695
    Clone ID Related Accession (Same CDS sequence) XM_010814783.2 , XM_010814782.1
    Accession Version XM_010814782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATACGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGAGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813084.1
    CDS134..3862
    Translation

    Target ORF information:

    RefSeq Version XM_010814782.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGATGG CGCGGGACGG CACACGGGAA GACGGCCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATTGAG
    AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCAGAGCTGG CGGCCTCAGT GCGGAAGAAG
    CTGGGCGAGA TCCGGCAGTG CTGGGCGGAG CTGGAAAGCA CCACTCAGGC CAAGGCGAGG
    CAGCTCTTTG AGGCCAGTAA GGCTGACCAA CTGGTGCAGA GCTTCGCGGA GCTGGACAAG
    AAACTCCTTC ACATGGAAAG CCAGCTGCAA GACGTGGATC CTGGTGGGGA CCTGGCCACT
    GTCAACAGCC AGCTGAAGAA GCTGCAGTCG ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGGG AGTTGCAGGC GCAGACGGCA GCGCTGCCCC TGGAGCCAGC GAGCAAGGAG
    CTGGTGGGCG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGCTG
    CAGGAGCGCC GCCGCCTGCT GCTCGCCTCC AAGGAGCTGC ACCAGGTGGC GCACGACCTC
    GAGGACGAGC TGGCATGGGT CCAGGAGCGA CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AGTGGTTTGC AGGCCGTCCA GCAGTACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCACG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCCCT GGCGTCGCTG
    CGCAGCCCCG AGGCTGAGGC CGTGCGCCGC GGCCAGGAGC AGCTGCAGGG CGCCTGGGCC
    GGGCTGCGGG AGGCGGCCGA GCGGCGGCAG CAGGTGCTCG ACGCCGCCTT CCAGGTGGAA
    CAGTACTACT TCGACGTGGC CGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAGCACCTG
    CAGCTGGAGC AGGGCGTCGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TATACGTGGC GCTCAAGGAG CTGGGCGAGG AACGCCGGGT GGGCCTGGAG
    CAGCAGTACT GGCTGTACCA GCTCAGTCGC CAGGTGGACG AGCTCGAGCA CTGGATCGCA
    GAGAAGGAGG TGGTGGCCGG CTCTCCTGAG CTCGGCCAGG ACTTTGAGCA TGTCACGGTG
    CTACAGGAGA AATTCTCAGA ATTCGCCAGC GAGACTGGCA CGGCAGGGCG GGAGCGGCTG
    GCGGCCGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCACACGGC GGCGGCCACC
    ATGGCCGAGT GGAAGGACGG GCTGAATGAG GCCTGGGCTG AGCTGCTGGA GCTTATGGGC
    ACGAGGGCCC AACTGCTGGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG TGATGCCCGA
    GAACTCCAAG GACAGATTGA GGAGAAGCGG AGGCGACTGC CTCGCCTGAC CACCCCGCCA
    GAGCCGAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GCGCCTTTGA ACATGACCTG
    CAGCTCCTTG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CAGCCCAGCT GCGGACGGTG
    TACGCGGGCG AGCACGCGGA GGCCATCGCC AGCCGGGAGC AGGAGGTGCT GCAAGGCTGG
    AAGGAGCTGC TGGCAGCCTG CGAGGACGCC CGCCTGCACG TGAGCTCCAC GGCCGACGCC
    CTGCGATTCC ACAACCAGGC CCGAGACCTG CTCTCCTGGA TGGATGGCAT CGCTGGCCAG
    ATTGGGGCAG CTGACAAGCC CAGGGACGTG TCATCGGTGG AGGTGCTCAT GAACTACCAC
    CAGGGCCTGA AGACTGAACT GGAGGCACGG ATGCCAGAGC TGACAGCCTG CCAGGAGCTG
    GGGCGCTCTC TGCTGCTCAA CAAGAGTGCC ATGGCTGACG AGATCCAGGC ACAGCTGGAC
    AAGCTGGGGA CCAGGAAGGA GGAAGTGTCG GATAAGTGGG ACCGCCACTG GGAGTGGCTG
    CAGCAGATGC TGGAAGTGCA CCAGTTTGCC CAAGAGGCAG TGGTGGCCGA TGCCTGGCTG
    ACAGCCCAGG AGCCGCTTTT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAAGTGGAA
    CAGCTTATCC GGCGGCACGA GGCCTTCCGC AAAGCGGCCG CAGCCTGGGA AGAAAGGTTC
    AGCTCTCTGC GGCGCCTGAC CACGATTGAG AAACTCAAGG CAGAACAGAG CAAGCAGCCG
    CCCACCCCCC TCCTGGGGCG CAAGTTCTTT GGAGACCCTA CGGAACTGGC GGCCAAGGCC
    GCGCCCCTGC TGCGGCCAGG GGGCTCTGAG AGGGGCTTGG AGCCGCTGGC CCGCCGGGCC
    TCAGACACGC TCTCGGCTGA GGTGCGGACC CGGGTGGGGT ACGTGCGGCA GGAGCTCAAG
    CCGGAGCGCC TCCAGCCGCG CATCGACCGG CTGCCCGAGG TCCCGGGGAG GGTGGAGCCT
    ACGGCCCAGC CGGCGGCACC AGAGGACGCC GCTGAGACCC CCGGGGCCCC TGCGGTAGTC
    GCAGCGGAGC AGGTCCGGCC GCGGCCCGAG CGGCAGGAGT CAGCTGATCG CGCGGAGGAG
    CTGCCCAGGA GGAGGCGGCC CGAGCGGCAG GAATCAGCCG ATCAATCCGA GGAGGCTGCG
    CGGAGGCGGC GGCCAGAGCG GCAGGAGTCA ACGGAGCACG AGGTGGCACA CAGCCTTACC
    CTGGGCCGCT ACGAGCAGAT GGAGAGGCGG CGGGAGCGGC GGGAGCGGCG GTTGGAGCGG
    CAGGAGTCCA GCGAACAGGA GATGCCCATC AGAGGAGACC TGGCCAAGGG GAAGGCCACC
    CTGGCTGACA TTGTGGAGCA ACTGCAGGAG AAAGAGGCAG GCCCAGGGCT CCCTGTTGGG
    CCTTCGCTGC CTCAGCCTCG CGAACTTCCC CCCGGCCGCC TGCCCAACGG GCTTGAGCCG
    CCCGAGCGGA CACCTCGGCC GGACCGGCCG CGGGCTCGGG ACCGGCCCAA GCCGAGACGG
    CGGCCGCGGC CCCGAGAGGG TGGTGAGGGC GGGGGAAGCC GGCGCTCGCG CTCCGCCCCG
    GCCCAGGGCG GCTCCGCCCC CGCGCCTCCA CCACCGCCCA CTCATACAGT GCAGCACGAG
    GGCTTCCTGC TGCGCAAGCG CGAGCTCGAC GCTAACCGCA AGTCGTCCAA CCGGTCATGG
    GTGAGCCTGT ACTGCGTGCT CAGCAAGGGG GAGCTGGGTT TCTACAAGGA CGCCAAGGGT
    CCGGCATCCG GGGGCACACA CGGCGGGGAG CCGCTGCTCA GCCTGCACAA GGCCACCAGC
    GAGGTGGCTA GTGACTACAA GAAAAAGAAG CATGTCTTCA AGCTCCAGAC CCAGGACGGC
    AGTGAGTTTT TGCTCCAGGC TAAAGATGAG GAGGAGATGA ACGGCTGGCT GGAGGCTGTG
    GCCGCCTCGG TTGGGGAACA CGCCGAGATC GCCCGCTGGG GCCAGACACA ACCCACCACA
    TCGTCCACAG ACGAGGGCAA CCCCAAGCGG GAGGCAGGGG AGCGCAGGGC CAGCGGGCGC
    CGGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.