TLK1 cDNA ORF clone, Bos taurus(cattle)

The following TLK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa16367 XM_015462257.1
Latest version!
Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa16367 XM_002685281.5
Latest version!
Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53681 XM_015462258.1
Latest version!
Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53681 XM_010801927.2
Latest version!
Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa227662 XM_024980179.1
Latest version!
Bos taurus tousled like kinase 1 (TLK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OBa16367 XM_002685281.4 Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53681 XM_010801927.1 Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa16367
    Clone ID Related Accession (Same CDS sequence) XM_002685281.4 , XM_002685281.5 , XM_015462257.1
    Accession Version XM_002685281.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002685281.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002685327.1
    CDS132..2432
    Translation

    Target ORF information:

    RefSeq Version XM_002685281.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002685281.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16367
    Clone ID Related Accession (Same CDS sequence) XM_002685281.4 , XM_002685281.5 , XM_015462257.1
    Accession Version XM_002685281.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002685281.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002685327.1
    CDS681..2981
    Translation

    Target ORF information:

    RefSeq Version XM_002685281.5
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002685281.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16367
    Clone ID Related Accession (Same CDS sequence) XM_002685281.4 , XM_002685281.5 , XM_015462257.1
    Accession Version XM_015462257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317743.1
    CDS132..2432
    Translation

    Target ORF information:

    RefSeq Version XM_015462257.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCGT TAGCTTTTGG GGACCACCCT
    ATTGTACAGC CAAAGCAGTT ATCCTTTAAA ATTACTCAGA CTGATCTTAC GATGCTGAAA
    TTAGCAGCAT TAGAAAGTAA TAAAAACCAG GACCTGGAAA AGAAGGAAGG TCGCATAGAT
    GACTTGCTCA GGGCTAATTG TGATCTCAGA CGGCAGATAG ATGAGCAACA AAAGCTACTT
    GAAAAATACA AAGAGCGATT AAATAAGTGC ATATCAATGA GCAAGAAACT GCTTATTGAA
    AAGAGTACAC AAGAAAAACT GTCAAGCCGA GAGAAGAGCA TGCAAGACAG ATTACGCCTT
    GGGCATTTCA CAACAGTTAG ACATGGTGCT TCATTTACTG AACAATGGAC AGATGGTTTT
    GCATTTCAGA ATCTTGTAAA GCAACAAGAA TGGGTGAATC AGCAAAGGGA AGATATTGAA
    AGGCAAAGGA AACTTCTAGC CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCC
    TCTACCAATT CTGAATCAAA ACAAAGGAAA AACAAAGCAG TCAATGGAGC AGAAAATGAT
    CCCTTCGTTA GACCAAATTT ACCACAACTG TTGACTTTGG CAGAATATCA TGAACAGGAA
    GAAATTTTCA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA
    CTTGAACGTT TGGAAAGAGT CAGAAATCTT CATATTCGAG AGCTCAAAAG AATAAATAAT
    GAAGATAATT CACAGTTCAA AGATCACCCA ACGTTAAATG AAAGATATTT ATTGCTCCAT
    CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA TATAAGGCTT TTGACCTTTA TGAACAAAGA
    TATGCTGCTG TGAAGATCCA CCAGCTTAAT AAAAGCTGGA GGGATGAGAA GAAGGAAAAC
    TACCACAAAC ATGCCTGCAG AGAGTATAGA ATACACAAAG AACTGGATCA CCCCAGAATA
    GTTAAACTCT ATGACTATTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GTTAGAATAC
    TGTGAAGGCA ATGACTTGGA TTTCTATCTG AAGCAACATA AGTTAATGTC AGAGAAAGAA
    GCTAGGTCTA TTGTAATGCA AATTGTAAAT GCACTAAGAT ATCTCAATGA AATAAAACCC
    CCTATTATAC ATTATGATCT TAAGCCAGGA AACATCCTCC TGGTAGATGG AACAGCATGT
    GGAGAAATAA AAATCACGGA TTTTGGTCTG TCCAAGATCA TGGATGATGA CAGCTATGGT
    GTAGATGGAA TGGATCTAAC ATCCCAGGGG GCAGGCACAT ACTGGTATTT ACCTCCCGAA
    TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG ATTTCCAACA AGGTTGACGT GTGGTCAGTT
    GGAGTCATCT TCTTTCAATG TCTTTATGGT AGAAAGCCAT TTGGTCACAA TCAATCTCAG
    CAAGACATTC TTCAAGAAAA TACGATATTA AAAGCCACTG ATGTCCAATT CCCTGTAAAA
    CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT ATAAGACGCT GTTTGGCATA CCGAAAAGAA
    GATAGGTTTG ATGTGCACCA ACTGGCAAAT GACCCCTACC TTCTCCCACA CATGAGAAGA
    TCCAATTCTT CAGGCAACTT ACACATGGCT GGGCTGACAG CATCACCCAC ACCCCCTTCT
    TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53681
    Clone ID Related Accession (Same CDS sequence) XM_015462258.1 , XM_010801927.2 , XM_010801927.1
    Accession Version XM_015462258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317744.1
    CDS132..2342
    Translation

    Target ORF information:

    RefSeq Version XM_015462258.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53681
    Clone ID Related Accession (Same CDS sequence) XM_015462258.1 , XM_010801927.2 , XM_010801927.1
    Accession Version XM_010801927.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800229.1
    CDS154..2364
    Translation

    Target ORF information:

    RefSeq Version XM_010801927.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53681
    Clone ID Related Accession (Same CDS sequence) XM_015462258.1 , XM_010801927.2 , XM_010801927.1
    Accession Version XM_010801927.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800229.1
    CDS132..2342
    Translation

    Target ORF information:

    RefSeq Version XM_010801927.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCAGCTCTCC 
    ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA
    TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCATA GTCTGGATCC AAGAAGGCAA
    GAATTATTGG AAGCTAGATT TACAGGAGTT GCAAGTGGGA GCACGGGGAG CACGGGCAGT
    TGCAGTGTTG GAGCTAAAGC CTCAACAAAT AATGAAAGCT CTAATCACAG TTTTGGAAGC
    TTGGGATCTT TGAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAATCATCC
    AGAGGAAGAA AAAGAAAAGC AGAAAACCAG AATGAAAGTA GTCAGGGAAA AAGTATTGGG
    GGACGTGGCC ACAAAATTAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCAAGTCCA
    GTAAGAGGCA TACCTCCTGC AATCCGTTCG CCTCAAAATT CACATTCACA TTCCACGCCT
    TCTTCATCTA CTGATCTTAC GATGCTGAAA TTAGCAGCAT TAGAAAGTAA TAAAAACCAG
    GACCTGGAAA AGAAGGAAGG TCGCATAGAT GACTTGCTCA GGGCTAATTG TGATCTCAGA
    CGGCAGATAG ATGAGCAACA AAAGCTACTT GAAAAATACA AAGAGCGATT AAATAAGTGC
    ATATCAATGA GCAAGAAACT GCTTATTGAA AAGAGTACAC AAGAAAAACT GTCAAGCCGA
    GAGAAGAGCA TGCAAGACAG ATTACGCCTT GGGCATTTCA CAACAGTTAG ACATGGTGCT
    TCATTTACTG AACAATGGAC AGATGGTTTT GCATTTCAGA ATCTTGTAAA GCAACAAGAA
    TGGGTGAATC AGCAAAGGGA AGATATTGAA AGGCAAAGGA AACTTCTAGC CAAACGCAAA
    CCTCCCACAG CTAATAATTC TCAGGCACCC TCTACCAATT CTGAATCAAA ACAAAGGAAA
    AACAAAGCAG TCAATGGAGC AGAAAATGAT CCCTTCGTTA GACCAAATTT ACCACAACTG
    TTGACTTTGG CAGAATATCA TGAACAGGAA GAAATTTTCA AACTTAGACT AGGACATCTC
    AAAAAGGAAG AGGCAGAAAT CCAGGCAGAA CTTGAACGTT TGGAAAGAGT CAGAAATCTT
    CATATTCGAG AGCTCAAAAG AATAAATAAT GAAGATAATT CACAGTTCAA AGATCACCCA
    ACGTTAAATG AAAGATATTT ATTGCTCCAT CTGCTTGGTA GAGGTGGCTT TAGTGAAGTA
    TATAAGGCTT TTGACCTTTA TGAACAAAGA TATGCTGCTG TGAAGATCCA CCAGCTTAAT
    AAAAGCTGGA GGGATGAGAA GAAGGAAAAC TACCACAAAC ATGCCTGCAG AGAGTATAGA
    ATACACAAAG AACTGGATCA CCCCAGAATA GTTAAACTCT ATGACTATTT CTCCTTGGAT
    ACAGATACGT TTTGTACAGT GTTAGAATAC TGTGAAGGCA ATGACTTGGA TTTCTATCTG
    AAGCAACATA AGTTAATGTC AGAGAAAGAA GCTAGGTCTA TTGTAATGCA AATTGTAAAT
    GCACTAAGAT ATCTCAATGA AATAAAACCC CCTATTATAC ATTATGATCT TAAGCCAGGA
    AACATCCTCC TGGTAGATGG AACAGCATGT GGAGAAATAA AAATCACGGA TTTTGGTCTG
    TCCAAGATCA TGGATGATGA CAGCTATGGT GTAGATGGAA TGGATCTAAC ATCCCAGGGG
    GCAGGCACAT ACTGGTATTT ACCTCCCGAA TGTTTCGTAG TTGGAAAGGA ACCGCCAAAG
    ATTTCCAACA AGGTTGACGT GTGGTCAGTT GGAGTCATCT TCTTTCAATG TCTTTATGGT
    AGAAAGCCAT TTGGTCACAA TCAATCTCAG CAAGACATTC TTCAAGAAAA TACGATATTA
    AAAGCCACTG ATGTCCAATT CCCTGTAAAA CCTGTCGTAA GCAGTGAAGC AAAGGCCTTT
    ATAAGACGCT GTTTGGCATA CCGAAAAGAA GATAGGTTTG ATGTGCACCA ACTGGCAAAT
    GACCCCTACC TTCTCCCACA CATGAGAAGA TCCAATTCTT CAGGCAACTT ACACATGGCT
    GGGCTGACAG CATCACCCAC ACCCCCTTCT TCAAGCATAC TTACTTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227662
    Clone ID Related Accession (Same CDS sequence) XM_024980179.1
    Accession Version XM_024980179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGATGAGC TTCATAGTCT GGATCCAAGA AGGCAAGAAT TATTGGAAGC TAGATTTACA 
    GGAGTTGCAA GTGGGAGCAC GGGGAGCACG GGCAGTTGCA GTGTTGGAGC TAAAGCCTCA
    ACAAATAATG AAAGCTCTAA TCACAGTTTT GGAAGCTTGG GATCTTTGAG TGACAAAGAA
    TCAGAGACAC CAGAGAAGAA ACAATCAGAA TCATCCAGAG GAAGAAAAAG AAAAGCAGAA
    AACCAGAATG AAAGTAGTCA GGGAAAAAGT ATTGGGGGAC GTGGCCACAA AATTAGCGAC
    TATTTTGAAT ACCAAGGTGG AAATGGCTCA AGTCCAGTAA GAGGCATACC TCCTGCAATC
    CGTTCGCCTC AAAATTCACA TTCACATTCC ACGCCTTCTT CATCTGTTCG ACCGAATAGC
    CCTTCTCCTA CTGCGTTAGC TTTTGGGGAC CACCCTATTG TACAGCCAAA GCAGTTATCC
    TTTAAAATTA CTCAGACTGA TCTTACGATG CTGAAATTAG CAGCATTAGA AAGTAATAAA
    AACCAGGACC TGGAAAAGAA GGAAGGTCGC ATAGATGACT TGCTCAGGGC TAATTGTGAT
    CTCAGACGGC AGATAGATGA GCAACAAAAG CTACTTGAAA AATACAAAGA GCGATTAAAT
    AAGTGCATAT CAATGAGCAA GAAACTGCTT ATTGAAAAGA GTACACAAGA AAAACTGTCA
    AGCCGAGAGA AGAGCATGCA AGACAGATTA CGCCTTGGGC ATTTCACAAC AGTTAGACAT
    GGTGCTTCAT TTACTGAACA ATGGACAGAT GGTTTTGCAT TTCAGAATCT TGTAAAGCAA
    CAAGAATGGG TGAATCAGCA AAGGGAAGAT ATTGAAAGGC AAAGGAAACT TCTAGCCAAA
    CGCAAACCTC CCACAGCTAA TAATTCTCAG GCACCCTCTA CCAATTCTGA ATCAAAACAA
    AGGAAAAACA AAGCAGTCAA TGGAGCAGAA AATGATCCCT TCGTTAGACC AAATTTACCA
    CAACTGTTGA CTTTGGCAGA ATATCATGAA CAGGAAGAAA TTTTCAAACT TAGACTAGGA
    CATCTCAAAA AGGAAGAGGC AGAAATCCAG GCAGAACTTG AACGTTTGGA AAGAGTCAGA
    AATCTTCATA TTCGAGAGCT CAAAAGAATA AATAATGAAG ATAATTCACA GTTCAAAGAT
    CACCCAACGT TAAATGAAAG ATATTTATTG CTCCATCTGC TTGGTAGAGG TGGCTTTAGT
    GAAGTATATA AGGCTTTTGA CCTTTATGAA CAAAGATATG CTGCTGTGAA GATCCACCAG
    CTTAATAAAA GCTGGAGGGA TGAGAAGAAG GAAAACTACC ACAAACATGC CTGCAGAGAG
    TATAGAATAC ACAAAGAACT GGATCACCCC AGAATAGTTA AACTCTATGA CTATTTCTCC
    TTGGATACAG ATACGTTTTG TACAGTGTTA GAATACTGTG AAGGCAATGA CTTGGATTTC
    TATCTGAAGC AACATAAGTT AATGTCAGAG AAAGAAGCTA GGTCTATTGT AATGCAAATT
    GTAAATGCAC TAAGATATCT CAATGAAATA AAACCCCCTA TTATACATTA TGATCTTAAG
    CCAGGAAACA TCCTCCTGGT AGATGGAACA GCATGTGGAG AAATAAAAAT CACGGATTTT
    GGTCTGTCCA AGATCATGGA TGATGACAGC TATGGTGTAG ATGGAATGGA TCTAACATCC
    CAGGGGGCAG GCACATACTG GTATTTACCT CCCGAATGTT TCGTAGTTGG AAAGGAACCG
    CCAAAGATTT CCAACAAGGT TGACGTGTGG TCAGTTGGAG TCATCTTCTT TCAATGTCTT
    TATGGTAGAA AGCCATTTGG TCACAATCAA TCTCAGCAAG ACATTCTTCA AGAAAATACG
    ATATTAAAAG CCACTGATGT CCAATTCCCT GTAAAACCTG TCGTAAGCAG TGAAGCAAAG
    GCCTTTATAA GACGCTGTTT GGCATACCGA AAAGAAGATA GGTTTGATGT GCACCAACTG
    GCAAATGACC CCTACCTTCT CCCACACATG AGAAGATCCA ATTCTTCAGG CAACTTACAC
    ATGGCTGGGC TGACAGCATC ACCCACACCC CCTTCTTCAA GCATACTTAC TTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835947.1
    CDS108..2264
    Translation

    Target ORF information:

    RefSeq Version XM_024980179.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 1 (TLK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGATGAGC TTCATAGTCT GGATCCAAGA AGGCAAGAAT TATTGGAAGC TAGATTTACA 
    GGAGTTGCAA GTGGGAGCAC GGGGAGCACG GGCAGTTGCA GTGTTGGAGC TAAAGCCTCA
    ACAAATAATG AAAGCTCTAA TCACAGTTTT GGAAGCTTGG GATCTTTGAG TGACAAAGAA
    TCAGAGACAC CAGAGAAGAA ACAATCAGAA TCATCCAGAG GAAGAAAAAG AAAAGCAGAA
    AACCAGAATG AAAGTAGTCA GGGAAAAAGT ATTGGGGGAC GTGGCCACAA AATTAGCGAC
    TATTTTGAAT ACCAAGGTGG AAATGGCTCA AGTCCAGTAA GAGGCATACC TCCTGCAATC
    CGTTCGCCTC AAAATTCACA TTCACATTCC ACGCCTTCTT CATCTGTTCG ACCGAATAGC
    CCTTCTCCTA CTGCGTTAGC TTTTGGGGAC CACCCTATTG TACAGCCAAA GCAGTTATCC
    TTTAAAATTA CTCAGACTGA TCTTACGATG CTGAAATTAG CAGCATTAGA AAGTAATAAA
    AACCAGGACC TGGAAAAGAA GGAAGGTCGC ATAGATGACT TGCTCAGGGC TAATTGTGAT
    CTCAGACGGC AGATAGATGA GCAACAAAAG CTACTTGAAA AATACAAAGA GCGATTAAAT
    AAGTGCATAT CAATGAGCAA GAAACTGCTT ATTGAAAAGA GTACACAAGA AAAACTGTCA
    AGCCGAGAGA AGAGCATGCA AGACAGATTA CGCCTTGGGC ATTTCACAAC AGTTAGACAT
    GGTGCTTCAT TTACTGAACA ATGGACAGAT GGTTTTGCAT TTCAGAATCT TGTAAAGCAA
    CAAGAATGGG TGAATCAGCA AAGGGAAGAT ATTGAAAGGC AAAGGAAACT TCTAGCCAAA
    CGCAAACCTC CCACAGCTAA TAATTCTCAG GCACCCTCTA CCAATTCTGA ATCAAAACAA
    AGGAAAAACA AAGCAGTCAA TGGAGCAGAA AATGATCCCT TCGTTAGACC AAATTTACCA
    CAACTGTTGA CTTTGGCAGA ATATCATGAA CAGGAAGAAA TTTTCAAACT TAGACTAGGA
    CATCTCAAAA AGGAAGAGGC AGAAATCCAG GCAGAACTTG AACGTTTGGA AAGAGTCAGA
    AATCTTCATA TTCGAGAGCT CAAAAGAATA AATAATGAAG ATAATTCACA GTTCAAAGAT
    CACCCAACGT TAAATGAAAG ATATTTATTG CTCCATCTGC TTGGTAGAGG TGGCTTTAGT
    GAAGTATATA AGGCTTTTGA CCTTTATGAA CAAAGATATG CTGCTGTGAA GATCCACCAG
    CTTAATAAAA GCTGGAGGGA TGAGAAGAAG GAAAACTACC ACAAACATGC CTGCAGAGAG
    TATAGAATAC ACAAAGAACT GGATCACCCC AGAATAGTTA AACTCTATGA CTATTTCTCC
    TTGGATACAG ATACGTTTTG TACAGTGTTA GAATACTGTG AAGGCAATGA CTTGGATTTC
    TATCTGAAGC AACATAAGTT AATGTCAGAG AAAGAAGCTA GGTCTATTGT AATGCAAATT
    GTAAATGCAC TAAGATATCT CAATGAAATA AAACCCCCTA TTATACATTA TGATCTTAAG
    CCAGGAAACA TCCTCCTGGT AGATGGAACA GCATGTGGAG AAATAAAAAT CACGGATTTT
    GGTCTGTCCA AGATCATGGA TGATGACAGC TATGGTGTAG ATGGAATGGA TCTAACATCC
    CAGGGGGCAG GCACATACTG GTATTTACCT CCCGAATGTT TCGTAGTTGG AAAGGAACCG
    CCAAAGATTT CCAACAAGGT TGACGTGTGG TCAGTTGGAG TCATCTTCTT TCAATGTCTT
    TATGGTAGAA AGCCATTTGG TCACAATCAA TCTCAGCAAG ACATTCTTCA AGAAAATACG
    ATATTAAAAG CCACTGATGT CCAATTCCCT GTAAAACCTG TCGTAAGCAG TGAAGCAAAG
    GCCTTTATAA GACGCTGTTT GGCATACCGA AAAGAAGATA GGTTTGATGT GCACCAACTG
    GCAAATGACC CCTACCTTCT CCCACACATG AGAAGATCCA ATTCTTCAGG CAACTTACAC
    ATGGCTGGGC TGACAGCATC ACCCACACCC CCTTCTTCAA GCATACTTAC TTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.