IFT122 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFT122
Entrez Gene ID	536731
Full Name	intraflagellar transport 122
General protein information	Preferred Names intraflagellar transport 122 Names intraflagellar transport protein 122 homolog WD repeat domain 10 intraflagellar transport 122 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005223076.3	XP_005223133.1	intraflagellar transport protein 122 homolog isoform X3
XM_024982901.1	XP_024838669.1	intraflagellar transport protein 122 homolog isoform X1
XM_005223076.4	XP_005223133.2	intraflagellar transport protein 122 homolog isoform X2
XM_010817917.3	XP_010816219.2	intraflagellar transport protein 122 homolog isoform X3
NM_001206592.1	NP_001193521.1	intraflagellar transport protein 122 homolog
XM_015459670.1	XP_015315156.1	intraflagellar transport protein 122 homolog isoform X2
XM_010817917.2	XP_010816219.1	intraflagellar transport protein 122 homolog isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ift122	NP_112454.2
Caenorhabditis elegans (roundworm)	daf-10	NP_501591.2
Xenopus tropicalis (tropical clawed frog)	ift122	NP_001106492.1
Pan troglodytes (chimpanzee)	IFT122	XP_516739.2
Rattus norvegicus (Norway rat)	Ift122	XP_006237129.1
Drosophila melanogaster (fruit fly)	Oseg1	NP_648221.3
Homo sapiens (human)	IFT122	NP_443715.1
Canis lupus familiaris (dog)	IFT122	XP_005632188.1
	IFT122	XP_002798754.1
Bos taurus (cattle)	IFT122	NP_001193521.1
Gallus gallus (chicken)	IFT122	XP_414454.4
Danio rerio (zebrafish)	ift122	NP_998055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006535	XP_316573.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IFT122 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFT122 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229059	XM_024982901.1 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229060	XM_005223076.4 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229061	XM_010817917.3 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa94466	XM_015459670.1 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa39692	NM_001206592.1 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa31748	XM_005223076.3 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa53439	XM_010817917.2 Latest version!	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFT122 ( XM_024982901.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838669.1 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229059
Clone ID Related Accession (Same CDS sequence)	XM_024982901.1
Accession Version	XM_024982901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGCCCCT CCCTTGCCTG TGTCTGCAGA GGTGCTGCAC 
CTGGTTGAAT TCTACCTGGA GGAGGGGATC ACAGACGAAG AAGCCGTCTC CCTCATTGAC 
CTGGAGGCGC CAAGACTCAA GCGGGAGAAC AAGTGGCAAG AGATCACGAG CAACAATGCC 
CAGACCCTGA GGCTGGACGA GAGCATGGAC TCCGTGGGGG ACGACGACCC CTTCACAGCC 
AAGCTGAGCT TTGAGCAAGG CGGCTCGGAG TTCGTGCCCG TGGTGGTGAG CCGCTCCGTG 
CTGCGCTCCA TGAGCCGGCG GGACGTCCTC ATCAAGCGCT GGCCGCCGCC CCTGCGGTGG 
CAGTACTTCC GCTCGCTGCT GCCCGACGCC TCCATCACCA TGTGCCCCTC CTGCTTCCAG 
ATGTTCCACT CGGAGGACTA CGAGCTGCTG CTGCTTCAGC ACACCTGCTG CCCTTACTGC 
CGGCGGCGCA TGGATGACCC CAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838669.1
CDS	119..3868
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSSRWENFWMNIEIEDSEEAIVSRYFQDIPSTLKSAVYSSQGSEAEEDEPEEEDNGAGDDSLEERNDILAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYAPPLPVSAEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_024982901.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982901.1

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGCCCCT CCCTTGCCTG TGTCTGCAGA GGTGCTGCAC 
CTGGTTGAAT TCTACCTGGA GGAGGGGATC ACAGACGAAG AAGCCGTCTC CCTCATTGAC 
CTGGAGGCGC CAAGACTCAA GCGGGAGAAC AAGTGGCAAG AGATCACGAG CAACAATGCC 
CAGACCCTGA GGCTGGACGA GAGCATGGAC TCCGTGGGGG ACGACGACCC CTTCACAGCC 
AAGCTGAGCT TTGAGCAAGG CGGCTCGGAG TTCGTGCCCG TGGTGGTGAG CCGCTCCGTG 
CTGCGCTCCA TGAGCCGGCG GGACGTCCTC ATCAAGCGCT GGCCGCCGCC CCTGCGGTGG 
CAGTACTTCC GCTCGCTGCT GCCCGACGCC TCCATCACCA TGTGCCCCTC CTGCTTCCAG 
ATGTTCCACT CGGAGGACTA CGAGCTGCTG CTGCTTCAGC ACACCTGCTG CCCTTACTGC 
CGGCGGCGCA TGGATGACCC CAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( XM_005223076.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223133.2 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229060
Clone ID Related Accession (Same CDS sequence)	XM_005223076.4
Accession Version	XM_005223076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGAGGTG CTGCACCTGG TTGAATTCTA CCTGGAGGAG 
GGGATCACAG ACGAAGAAGC CGTCTCCCTC ATTGACCTGG AGGCGCCAAG ACTCAAGCGG 
GAGAACAAGT GGCAAGAGAT CACGAGCAAC AATGCCCAGA CCCTGAGGCT GGACGAGAGC 
ATGGACTCCG TGGGGGACGA CGACCCCTTC ACAGCCAAGC TGAGCTTTGA GCAAGGCGGC 
TCGGAGTTCG TGCCCGTGGT GGTGAGCCGC TCCGTGCTGC GCTCCATGAG CCGGCGGGAC 
GTCCTCATCA AGCGCTGGCC GCCGCCCCTG CGGTGGCAGT ACTTCCGCTC GCTGCTGCCC 
GACGCCTCCA TCACCATGTG CCCCTCCTGC TTCCAGATGT TCCACTCGGA GGACTACGAG 
CTGCTGCTGC TTCAGCACAC CTGCTGCCCT TACTGCCGGC GGCGCATGGA TGACCCCAGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223133.2
CDS	119..3844
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSSRWENFWMNIEIEDSEEAIVSRYFQDIPSTLKSAVYSSQGSEAEEDEPEEEDNGAGDDSLEERNDILAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_005223076.4
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223076.4

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGAGGTG CTGCACCTGG TTGAATTCTA CCTGGAGGAG 
GGGATCACAG ACGAAGAAGC CGTCTCCCTC ATTGACCTGG AGGCGCCAAG ACTCAAGCGG 
GAGAACAAGT GGCAAGAGAT CACGAGCAAC AATGCCCAGA CCCTGAGGCT GGACGAGAGC 
ATGGACTCCG TGGGGGACGA CGACCCCTTC ACAGCCAAGC TGAGCTTTGA GCAAGGCGGC 
TCGGAGTTCG TGCCCGTGGT GGTGAGCCGC TCCGTGCTGC GCTCCATGAG CCGGCGGGAC 
GTCCTCATCA AGCGCTGGCC GCCGCCCCTG CGGTGGCAGT ACTTCCGCTC GCTGCTGCCC 
GACGCCTCCA TCACCATGTG CCCCTCCTGC TTCCAGATGT TCCACTCGGA GGACTACGAG 
CTGCTGCTGC TTCAGCACAC CTGCTGCCCT TACTGCCGGC GGCGCATGGA TGACCCCAGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( XM_010817917.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816219.2 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.

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CloneID	OBa229061
Clone ID Related Accession (Same CDS sequence)	XM_010817917.3
Accession Version	XM_010817917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGCC CCTCCCTTGC CTGTGTCTGC AGAGGTGCTG 
CACCTGGTTG AATTCTACCT GGAGGAGGGG ATCACAGACG AAGAAGCCGT CTCCCTCATT 
GACCTGGAGG CGCCAAGACT CAAGCGGGAG AACAAGTGGC AAGAGATCAC GAGCAACAAT 
GCCCAGACCC TGAGGCTGGA CGAGAGCATG GACTCCGTGG GGGACGACGA CCCCTTCACA 
GCCAAGCTGA GCTTTGAGCA AGGCGGCTCG GAGTTCGTGC CCGTGGTGGT GAGCCGCTCC 
GTGCTGCGCT CCATGAGCCG GCGGGACGTC CTCATCAAGC GCTGGCCGCC GCCCCTGCGG 
TGGCAGTACT TCCGCTCGCT GCTGCCCGAC GCCTCCATCA CCATGTGCCC CTCCTGCTTC 
CAGATGTTCC ACTCGGAGGA CTACGAGCTG CTGCTGCTTC AGCACACCTG CTGCCCTTAC 
TGCCGGCGGC GCATGGATGA CCCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816219.2
CDS	119..3691
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSREERNDILAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYAPPLPVSAEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_010817917.3
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817917.3

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGCC CCTCCCTTGC CTGTGTCTGC AGAGGTGCTG 
CACCTGGTTG AATTCTACCT GGAGGAGGGG ATCACAGACG AAGAAGCCGT CTCCCTCATT 
GACCTGGAGG CGCCAAGACT CAAGCGGGAG AACAAGTGGC AAGAGATCAC GAGCAACAAT 
GCCCAGACCC TGAGGCTGGA CGAGAGCATG GACTCCGTGG GGGACGACGA CCCCTTCACA 
GCCAAGCTGA GCTTTGAGCA AGGCGGCTCG GAGTTCGTGC CCGTGGTGGT GAGCCGCTCC 
GTGCTGCGCT CCATGAGCCG GCGGGACGTC CTCATCAAGC GCTGGCCGCC GCCCCTGCGG 
TGGCAGTACT TCCGCTCGCT GCTGCCCGAC GCCTCCATCA CCATGTGCCC CTCCTGCTTC 
CAGATGTTCC ACTCGGAGGA CTACGAGCTG CTGCTGCTTC AGCACACCTG CTGCCCTTAC 
TGCCGGCGGC GCATGGATGA CCCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( XM_015459670.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315156.1 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.

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CloneID	OBa94466
Clone ID Related Accession (Same CDS sequence)	XM_015459670.1
Accession Version	XM_015459670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATCATA AAAGGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGATCCTGC CCAGAAGGAA GTGATGCTCA GCAAGTTCCA CCACTTCCAG 
CACCTGGCCG AGCTGTACCA CGGCTACCAC GTCATCCACC GATACGTGGA GGAGCCATTC 
AGCTTCCACC TTCCCGAGAC GCTCTTCAAC ATCTCCAGGT TCCTGCTGCA CAGCCTGACC 
AAGGACACCC CCCTGGGCAT CTCCAAAGTG AACACACTCT TCACCCTGGC CAAACAGAGC 
AAGGCCCTGG GCGCCTACAA GCTGGCCCGG CACGCCTACG ACAAGCTGCA GGGCCTGCAG 
ATCCCCGCCC GTTTCCAGAA GTCCATTGAA CTGGGCAGCC TGACTATCCG CTCCAAGCCC 
TTCCATGACA GTGAGGAGCT GGTGCCCTTG TGCTACCGCT GCTCCACCAA CAACCCGCTG 
CTCAACAACC TGGGCAACGT CTGCATCAAC TGCCGCCAGC CCTTCGTCTT CTCCGCCTCT 
TCCTACGCCC CTCCCTTGCC TGTGTCTGCA GAGGTGCTGC ACCTGGTTGA ATTCTACCTG 
GAGGAGGGGA TCACAGACGA AGAAGCCGTC TCCCTCATTG ACCTGGAGGC GCCAAGACTC 
AAGCGGGAGA ACAAGTGGCA AGAGATCACG AGCAACAATG CCCAGACCCT GAGGCTGGAC 
GAGAGCATGG ACTCCGTGGG GGACGACGAC CCCTTCACAG CCAAGCTGAG CTTTGAGCAA 
GGCGGCTCGG AGTTCGTGCC CGTGGTGGTG AGCCGCTCCG TGCTGCGCTC CATGAGCCGG 
CGGGACGTCC TCATCAAGCG CTGGCCGCCG CCCCTGCGGT GGCAGTACTT CCGCTCGCTG 
CTGCCCGACG CCTCCATCAC CATGTGCCCC TCCTGCTTCC AGATGTTCCA CTCGGAGGAC 
TACGAGCTGC TGCTGCTTCA GCACACCTGC TGCCCTTACT GCCGGCGGCG CATGGATGAC 
CCCAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315156.1
CDS	110..3541
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSREERNHKRAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYAPPLPVSAEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_015459670.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459670.1

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATCATA AAAGGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGATCCTGC CCAGAAGGAA GTGATGCTCA GCAAGTTCCA CCACTTCCAG 
CACCTGGCCG AGCTGTACCA CGGCTACCAC GTCATCCACC GATACGTGGA GGAGCCATTC 
AGCTTCCACC TTCCCGAGAC GCTCTTCAAC ATCTCCAGGT TCCTGCTGCA CAGCCTGACC 
AAGGACACCC CCCTGGGCAT CTCCAAAGTG AACACACTCT TCACCCTGGC CAAACAGAGC 
AAGGCCCTGG GCGCCTACAA GCTGGCCCGG CACGCCTACG ACAAGCTGCA GGGCCTGCAG 
ATCCCCGCCC GTTTCCAGAA GTCCATTGAA CTGGGCAGCC TGACTATCCG CTCCAAGCCC 
TTCCATGACA GTGAGGAGCT GGTGCCCTTG TGCTACCGCT GCTCCACCAA CAACCCGCTG 
CTCAACAACC TGGGCAACGT CTGCATCAAC TGCCGCCAGC CCTTCGTCTT CTCCGCCTCT 
TCCTACGCCC CTCCCTTGCC TGTGTCTGCA GAGGTGCTGC ACCTGGTTGA ATTCTACCTG 
GAGGAGGGGA TCACAGACGA AGAAGCCGTC TCCCTCATTG ACCTGGAGGC GCCAAGACTC 
AAGCGGGAGA ACAAGTGGCA AGAGATCACG AGCAACAATG CCCAGACCCT GAGGCTGGAC 
GAGAGCATGG ACTCCGTGGG GGACGACGAC CCCTTCACAG CCAAGCTGAG CTTTGAGCAA 
GGCGGCTCGG AGTTCGTGCC CGTGGTGGTG AGCCGCTCCG TGCTGCGCTC CATGAGCCGG 
CGGGACGTCC TCATCAAGCG CTGGCCGCCG CCCCTGCGGT GGCAGTACTT CCGCTCGCTG 
CTGCCCGACG CCTCCATCAC CATGTGCCCC TCCTGCTTCC AGATGTTCCA CTCGGAGGAC 
TACGAGCTGC TGCTGCTTCA GCACACCTGC TGCCCTTACT GCCGGCGGCG CATGGATGAC 
CCCAGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( NM_001206592.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193521.1 Bos taurus intraflagellar transport 122 (IFT122), mRNA.

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CloneID	OBa39692
Clone ID Related Accession (Same CDS sequence)	NM_001206592.1
Accession Version	NM_001206592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1, BC151760.1, BE588612.1 and DY124385.1. On or before May 4, 2011 this sequence version replaced XM_875299.4, XM_002697103.1, XM_002697104.1, XM_002697105.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.51477.1, SRR5571262.31276.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGTAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAGTC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGAG GTGCTGCACC TGGTTGAATT CTACCTGGAG 
GAGGGGATCA CAGACGAAGA AGCCGTCTCC CTCATTGACC TGGAGGCGCC AAGACTCAAG 
CGGGAGAACA AGTGGCAAGA GATCACGAGC AACAATGCCC AGACCCTGAG GCTGGACGAG 
AGCATGGACT CCGTGGGGGA CGACGACCCC TTCACAGCCA AGCTGAGCTT TGAGCAAGGC 
GGCTCGGAGT TCGTGCCCGT GGTGGTGAGC CGCTCCGTGC TGCGCTCCAT GAGCCGGCGG 
GACGTCCTCA TCAAGCGCTG GCCGCCGCCC CTGCGGTGGC AGTACTTCCG CTCGCTGCTG 
CCCGACGCCT CCATCACCAT GTGCCCCTCC TGCTTCCAGA TGTTCCACTC GGAGGACTAC 
GAGCTGCTGC TGCTTCAGCA CACCTGCTGC CCTTACTGCC GGCGGCGCAT GGATGACCCC 
AGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193521.1
CDS	73..3621
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSREERNDILAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	NM_001206592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206592.1

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGTAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAGTC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGAG GTGCTGCACC TGGTTGAATT CTACCTGGAG 
GAGGGGATCA CAGACGAAGA AGCCGTCTCC CTCATTGACC TGGAGGCGCC AAGACTCAAG 
CGGGAGAACA AGTGGCAAGA GATCACGAGC AACAATGCCC AGACCCTGAG GCTGGACGAG 
AGCATGGACT CCGTGGGGGA CGACGACCCC TTCACAGCCA AGCTGAGCTT TGAGCAAGGC 
GGCTCGGAGT TCGTGCCCGT GGTGGTGAGC CGCTCCGTGC TGCGCTCCAT GAGCCGGCGG 
GACGTCCTCA TCAAGCGCTG GCCGCCGCCC CTGCGGTGGC AGTACTTCCG CTCGCTGCTG 
CCCGACGCCT CCATCACCAT GTGCCCCTCC TGCTTCCAGA TGTTCCACTC GGAGGACTAC 
GAGCTGCTGC TGCTTCAGCA CACCTGCTGC CCTTACTGCC GGCGGCGCAT GGATGACCCC 
AGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( XM_005223076.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223133.1 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.

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CloneID	OBa31748
Clone ID Related Accession (Same CDS sequence)	XM_005223076.3
Accession Version	XM_005223076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATCATAAAA GGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGAGGTG CTGCACCTGG TTGAATTCTA CCTGGAGGAG 
GGGATCACAG ACGAAGAAGC CGTCTCCCTC ATTGACCTGG AGGCGCCAAG ACTCAAGCGG 
GAGAACAAGT GGCAAGAGAT CACGAGCAAC AATGCCCAGA CCCTGAGGCT GGACGAGAGC 
ATGGACTCCG TGGGGGACGA CGACCCCTTC ACAGCCAAGC TGAGCTTTGA GCAAGGCGGC 
TCGGAGTTCG TGCCCGTGGT GGTGAGCCGC TCCGTGCTGC GCTCCATGAG CCGGCGGGAC 
GTCCTCATCA AGCGCTGGCC GCCGCCCCTG CGGTGGCAGT ACTTCCGCTC GCTGCTGCCC 
GACGCCTCCA TCACCATGTG CCCCTCCTGC TTCCAGATGT TCCACTCGGA GGACTACGAG 
CTGCTGCTGC TTCAGCACAC CTGCTGCCCT TACTGCCGGC GGCGCATGGA TGACCCCAGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223133.1
CDS	110..3835
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSSRWENFWMNIEIEDSEEAIVSRYFQDIPSTLKSAVYSSQGSEAEEDEPEEEDNGAGDDSLEERNHKRAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_005223076.3
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223076.3

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGCCGATGG GAGAATTTCT GGATGAACAT AGAGATCGAG 
GATTCCGAGG AAGCCATTGT CAGCAGATAC TTTCAGGATA TCCCTTCCAC TCTGAAGTCC 
GCAGTGTACA GTAGTCAGGG TAGTGAGGCA GAGGAGGACG AGCCCGAGGA AGAGGACAAC 
GGTGCCGGGG ACGACAGCCT AGAGGAACGC AATCATAAAA GGGCTGTAGC TGACTGGGGA 
CAGAAGCTTT CCTTCTACCA GCTGAGTGGA AAACAGATCG GGAAGGACCA CTCCCTGAAC 
TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGGGAGT ACATTTTGCT GGGGGGATCA 
GACAAGCAAG TGTCCCTTTT CACCAAGGAT GGTGTACGGC TTGGGGCCGT TGGGGAGCAG 
ACCTCCTGGG TGTGGACGTG TAAAGTGAAG CCTGACTCCA ACTACGTGGT GGTGGGCTGC 
CAGGATGGCA CCATTTCCTT CTACCAGCTG ATTTTCAGCA CAGTCCACGG GCTCTACAAG 
GACCGCTACG CCTACAGGGA TAGCATGACT GATGTCATTG TGCAGCACCT GATCACCGAG 
CAGAAAGTTC GGATTAAATG CAAAGAACTT GTCAAGAAAA TTGCCATCTA CAAGAATCGA 
TTAGCGATCC AGCTGCCCGA GAAGATCCTC ATCTACGAGC TGTACTCAGA GGACTCGCTG 
GACATGCGGT ACCGGGTGAA GGAGAAGATT GCCAGGAAGT TCGAGTGCAA CCTCCTGGTG 
GTGTGTGCCG ACCACATCAT CCTCTGTCAG GAGAGGCGGC TGCAGTGCCT GTCCTTCAGC 
GGCGTGAAGG AGCGGGAGTG GCAGATGGAG TCTCTCATTC GCTACATTAA AGTGATCGGT 
GGCCCCCCAG GAAGGGAGGG TCTGCTGGTC GGCCTGAAGA ATGGACAGAT CCTCAAGATC 
TTCGTGGACA ACCTCTTCGC CATCGTCCTG CTGAAGCAGG CCACCGCCGT GCGCTGCCTG 
GACATGAGCG CCTCCCGCAA CAAGTTGGCC GTGGTGGACG AGAACGACAT GTGCCTGGTG 
TACGACATCC ACACCAAGGA GCTGCTGTTT CAGGAACCCA ACGCCAACAG CGTGGCCTGG 
AACACCCAGT GTGAGGACAT GCTCTGCTTC TCTGGCGGTG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CGGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTCGGCTA CAACGGCTCC 
AAGATCTTCT GCCTCCACGT CTTCTCCATG TCGGCCGTGG AGGTGCCACA GTCTGCCCCG 
ATGTACCAGT ACCTGGACAG GAAGATGTTC CGGGAAGCCT ACCAGATCGC CTGCCTGGGC 
GTGACCGATG CCGACTGGCG CGAGCTGGCC ATGGAAGCCC TGGAAGGACT GGAGTTTGAG 
ACAGCGAAGA AGGCCTTCAC CAGAGTACAA GACCTCCGGT ATTTAGAGCT CATCAACAGC 
ATTGAGGAGC GGAAGAAGCG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTCCA CGAGGCCGCC AAACTGTACA AGAGGAGCGG GCACGAGAAC 
CTCGCGCTCG ACATGTACAC GGAACTGCGC ATGTTCGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGAAGACC CCAAAGAAAC AAAGATGCTA ATCACCAAAC AGGCTGACTG GGCTCGAAAC 
ATCAACGAGC CCAAAGCTGC CGTGGAGATG TACATCTCGG CGGGCGAGCA CTGCAAGGCC 
ATCGAGATCA GCGGGGAACA CGGCTGGGTC GACATGCTCA TCGACATTGC CCGCAAGCTG 
GACAAGGCCG AGCGGGAGCC GCTGCTGATG TGCGCCCACT TCTTCAAGAA GCTGGATAAC 
CCTGGCTATG CCGCTGAGAC CTACCTGAAG ATTGGTGACC TCAAATCCTT GGTTCAGCTG 
CACGTGGAGA CCCAGCGCTG GGATGAGGCC TTCGCCTTGG GTGAGAAGCA CCCCGAGTTT 
AAGGATGACA TCTACGTGCC CTATGCTCAG TGGCTCGCAG AGAATGATCG TTTTGAGGAA 
GCCCAGAAAG CGTTCCACAA GGCCGGGCGG CAGGGCGAGG CGGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCCGTGGT CGAGAGCAGG TTTAGTGACG CCGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA TATAGCTCAA GATCCTGCCC AGAAGGAAGT GATGCTCAGC 
AAGTTCCACC ACTTCCAGCA CCTGGCCGAG CTGTACCACG GCTACCACGT CATCCACCGA 
TACGTGGAGG AGCCATTCAG CTTCCACCTT CCCGAGACGC TCTTCAACAT CTCCAGGTTC 
CTGCTGCACA GCCTGACCAA GGACACCCCC CTGGGCATCT CCAAAGTGAA CACACTCTTC 
ACCCTGGCCA AACAGAGCAA GGCCCTGGGC GCCTACAAGC TGGCCCGGCA CGCCTACGAC 
AAGCTGCAGG GCCTGCAGAT CCCCGCCCGT TTCCAGAAGT CCATTGAACT GGGCAGCCTG 
ACTATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TGCCCTTGTG CTACCGCTGC 
TCCACCAACA ACCCGCTGCT CAACAACCTG GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCGTCTTCT CCGCCTCTTC CTACGAGGTG CTGCACCTGG TTGAATTCTA CCTGGAGGAG 
GGGATCACAG ACGAAGAAGC CGTCTCCCTC ATTGACCTGG AGGCGCCAAG ACTCAAGCGG 
GAGAACAAGT GGCAAGAGAT CACGAGCAAC AATGCCCAGA CCCTGAGGCT GGACGAGAGC 
ATGGACTCCG TGGGGGACGA CGACCCCTTC ACAGCCAAGC TGAGCTTTGA GCAAGGCGGC 
TCGGAGTTCG TGCCCGTGGT GGTGAGCCGC TCCGTGCTGC GCTCCATGAG CCGGCGGGAC 
GTCCTCATCA AGCGCTGGCC GCCGCCCCTG CGGTGGCAGT ACTTCCGCTC GCTGCTGCCC 
GACGCCTCCA TCACCATGTG CCCCTCCTGC TTCCAGATGT TCCACTCGGA GGACTACGAG 
CTGCTGCTGC TTCAGCACAC CTGCTGCCCT TACTGCCGGC GGCGCATGGA TGACCCCAGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT122 ( XM_010817917.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816219.1 Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.

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CloneID	OBa53439
Clone ID Related Accession (Same CDS sequence)	XM_010817917.2
Accession Version	XM_010817917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 122 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATCATA AAAGGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGCC CCTCCCTTGC CTGTGTCTGC AGAGGTGCTG 
CACCTGGTTG AATTCTACCT GGAGGAGGGG ATCACAGACG AAGAAGCCGT CTCCCTCATT 
GACCTGGAGG CGCCAAGACT CAAGCGGGAG AACAAGTGGC AAGAGATCAC GAGCAACAAT 
GCCCAGACCC TGAGGCTGGA CGAGAGCATG GACTCCGTGG GGGACGACGA CCCCTTCACA 
GCCAAGCTGA GCTTTGAGCA AGGCGGCTCG GAGTTCGTGC CCGTGGTGGT GAGCCGCTCC 
GTGCTGCGCT CCATGAGCCG GCGGGACGTC CTCATCAAGC GCTGGCCGCC GCCCCTGCGG 
TGGCAGTACT TCCGCTCGCT GCTGCCCGAC GCCTCCATCA CCATGTGCCC CTCCTGCTTC 
CAGATGTTCC ACTCGGAGGA CTACGAGCTG CTGCTGCTTC AGCACACCTG CTGCCCTTAC 
TGCCGGCGGC GCATGGATGA CCCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816219.1
CDS	110..3682
Translation	MRAVLTWRDKVEQCVYDLAFKPDGTQLIVAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDSIQCVSYNPITHQLASCSSSDIGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMFNGVVSIRNKNGEEKVKIERPGGSLSPIWSISWNPSREERNHKRAVADWGQKLSFYQLSGKQIGKDHSLNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGAVGEQTSWVWTCKVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYKNRLAIQLPEKILIYELYSEDSLDMRYRVKEKIARKFECNLLVVCADHIILCQERRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDMCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFREAYQIACLGVTDADWRELAMEALEGLEFETAKKAFTRVQDLRYLELINSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTELRMFEYAKDFLGSEDPKETKMLITKQADWARNINEPKAAVEMYISAGEHCKAIEISGEHGWVDMLIDIARKLDKAEREPLLMCAHFFKKLDNPGYAAETYLKIGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFSDAAYYYWMLSMQCLDIAQDPAQKEVMLSKFHHFQHLAELYHGYHVIHRYVEEPFSFHLPETLFNISRFLLHSLTKDTPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLQGLQIPARFQKSIELGSLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFVFSASSYAPPLPVSAEVLHLVEFYLEEGITDEEAVSLIDLEAPRLKRENKWQEITSNNAQTLRLDESMDSVGDDDPFTAKLSFEQGGSEFVPVVVSRSVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLLLQHTCCPYCRRRMDDPSP

Target ORF information:

RefSeq Version	XM_010817917.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 122 (IFT122), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817917.2

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGATAAA GTCGAACAGT GTGTATATGA CCTCGCGTTC 
AAGCCCGATG GAACCCAGCT GATTGTGGCT GCGGGAAACA GATTACTGGT TTATGACACA 
TCTGATGGCA CCTTGCTTCA GCCCCTAAAG GGACACAAGG ACACGGTATA CTGTGTGGCA 
TATGCGAAGG ATGGCAAACG CTTTGCTTCT GGATCAGCTG ACAAAAGTGT AATAATCTGG 
ACGTCAAAAC TGGAAGGCAT TCTGAAGTAC ACGCACAATG ACTCCATCCA GTGTGTCTCC 
TACAACCCCA TCACCCACCA GCTGGCATCC TGTTCCTCCA GCGACATCGG CTTATGGTCT 
CCTGAGCAGA AGTCTGTCTC TAAGCACAAA TCCAGCAGCA AGATAACCTG CTGCAGCTGG 
ACAAATGACG GGCAGTACCT GGCTCTGGGG ATGTTCAACG GGGTCGTCAG CATCCGCAAC 
AAGAACGGCG AGGAGAAGGT GAAGATCGAG CGGCCGGGGG GCTCCCTCTC CCCTATCTGG 
TCCATCAGCT GGAACCCATC CAGAGAGGAA CGCAATCATA AAAGGGCTGT AGCTGACTGG 
GGACAGAAGC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TCGGGAAGGA CCACTCCCTG 
AACTTTGACC CCTGCTGCAT CAGCTACTTT ACTAAAGGGG AGTACATTTT GCTGGGGGGA 
TCAGACAAGC AAGTGTCCCT TTTCACCAAG GATGGTGTAC GGCTTGGGGC CGTTGGGGAG 
CAGACCTCCT GGGTGTGGAC GTGTAAAGTG AAGCCTGACT CCAACTACGT GGTGGTGGGC 
TGCCAGGATG GCACCATTTC CTTCTACCAG CTGATTTTCA GCACAGTCCA CGGGCTCTAC 
AAGGACCGCT ACGCCTACAG GGATAGCATG ACTGATGTCA TTGTGCAGCA CCTGATCACC 
GAGCAGAAAG TTCGGATTAA ATGCAAAGAA CTTGTCAAGA AAATTGCCAT CTACAAGAAT 
CGATTAGCGA TCCAGCTGCC CGAGAAGATC CTCATCTACG AGCTGTACTC AGAGGACTCG 
CTGGACATGC GGTACCGGGT GAAGGAGAAG ATTGCCAGGA AGTTCGAGTG CAACCTCCTG 
GTGGTGTGTG CCGACCACAT CATCCTCTGT CAGGAGAGGC GGCTGCAGTG CCTGTCCTTC 
AGCGGCGTGA AGGAGCGGGA GTGGCAGATG GAGTCTCTCA TTCGCTACAT TAAAGTGATC 
GGTGGCCCCC CAGGAAGGGA GGGTCTGCTG GTCGGCCTGA AGAATGGACA GATCCTCAAG 
ATCTTCGTGG ACAACCTCTT CGCCATCGTC CTGCTGAAGC AGGCCACCGC CGTGCGCTGC 
CTGGACATGA GCGCCTCCCG CAACAAGTTG GCCGTGGTGG ACGAGAACGA CATGTGCCTG 
GTGTACGACA TCCACACCAA GGAGCTGCTG TTTCAGGAAC CCAACGCCAA CAGCGTGGCC 
TGGAACACCC AGTGTGAGGA CATGCTCTGC TTCTCTGGCG GTGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCGGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTCGG CTACAACGGC 
TCCAAGATCT TCTGCCTCCA CGTCTTCTCC ATGTCGGCCG TGGAGGTGCC ACAGTCTGCC 
CCGATGTACC AGTACCTGGA CAGGAAGATG TTCCGGGAAG CCTACCAGAT CGCCTGCCTG 
GGCGTGACCG ATGCCGACTG GCGCGAGCTG GCCATGGAAG CCCTGGAAGG ACTGGAGTTT 
GAGACAGCGA AGAAGGCCTT CACCAGAGTA CAAGACCTCC GGTATTTAGA GCTCATCAAC 
AGCATTGAGG AGCGGAAGAA GCGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT CCACGAGGCC GCCAAACTGT ACAAGAGGAG CGGGCACGAG 
AACCTCGCGC TCGACATGTA CACGGAACTG CGCATGTTCG AGTACGCCAA GGATTTCCTT 
GGATCTGAAG ACCCCAAAGA AACAAAGATG CTAATCACCA AACAGGCTGA CTGGGCTCGA 
AACATCAACG AGCCCAAAGC TGCCGTGGAG ATGTACATCT CGGCGGGCGA GCACTGCAAG 
GCCATCGAGA TCAGCGGGGA ACACGGCTGG GTCGACATGC TCATCGACAT TGCCCGCAAG 
CTGGACAAGG CCGAGCGGGA GCCGCTGCTG ATGTGCGCCC ACTTCTTCAA GAAGCTGGAT 
AACCCTGGCT ATGCCGCTGA GACCTACCTG AAGATTGGTG ACCTCAAATC CTTGGTTCAG 
CTGCACGTGG AGACCCAGCG CTGGGATGAG GCCTTCGCCT TGGGTGAGAA GCACCCCGAG 
TTTAAGGATG ACATCTACGT GCCCTATGCT CAGTGGCTCG CAGAGAATGA TCGTTTTGAG 
GAAGCCCAGA AAGCGTTCCA CAAGGCCGGG CGGCAGGGCG AGGCGGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCCGT GGTCGAGAGC AGGTTTAGTG ACGCCGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGATATAGCT CAAGATCCTG CCCAGAAGGA AGTGATGCTC 
AGCAAGTTCC ACCACTTCCA GCACCTGGCC GAGCTGTACC ACGGCTACCA CGTCATCCAC 
CGATACGTGG AGGAGCCATT CAGCTTCCAC CTTCCCGAGA CGCTCTTCAA CATCTCCAGG 
TTCCTGCTGC ACAGCCTGAC CAAGGACACC CCCCTGGGCA TCTCCAAAGT GAACACACTC 
TTCACCCTGG CCAAACAGAG CAAGGCCCTG GGCGCCTACA AGCTGGCCCG GCACGCCTAC 
GACAAGCTGC AGGGCCTGCA GATCCCCGCC CGTTTCCAGA AGTCCATTGA ACTGGGCAGC 
CTGACTATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTGCCCTT GTGCTACCGC 
TGCTCCACCA ACAACCCGCT GCTCAACAAC CTGGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCGTCT TCTCCGCCTC TTCCTACGCC CCTCCCTTGC CTGTGTCTGC AGAGGTGCTG 
CACCTGGTTG AATTCTACCT GGAGGAGGGG ATCACAGACG AAGAAGCCGT CTCCCTCATT 
GACCTGGAGG CGCCAAGACT CAAGCGGGAG AACAAGTGGC AAGAGATCAC GAGCAACAAT 
GCCCAGACCC TGAGGCTGGA CGAGAGCATG GACTCCGTGG GGGACGACGA CCCCTTCACA 
GCCAAGCTGA GCTTTGAGCA AGGCGGCTCG GAGTTCGTGC CCGTGGTGGT GAGCCGCTCC 
GTGCTGCGCT CCATGAGCCG GCGGGACGTC CTCATCAAGC GCTGGCCGCC GCCCCTGCGG 
TGGCAGTACT TCCGCTCGCT GCTGCCCGAC GCCTCCATCA CCATGTGCCC CTCCTGCTTC 
CAGATGTTCC ACTCGGAGGA CTACGAGCTG CTGCTGCTTC AGCACACCTG CTGCCCTTAC 
TGCCGGCGGC GCATGGATGA CCCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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IFT122 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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