NOS1 cDNA ORF clone, Bos taurus(cattle)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224633 XM_024977752.1
Latest version!
Bos taurus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa224634 XM_024977753.1
Latest version!
Bos taurus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa93237 XM_015475535.1
Latest version!
Bos taurus nitric oxide synthase 1 (NOS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93237 XM_015467111.1
Latest version!
Bos taurus nitric oxide synthase 1 (NOS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224633
    Clone ID Related Accession (Same CDS sequence) XM_024977752.1
    Accession Version XM_024977752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC GGCCCCTGGG TCCCCCCACA GCAGCCGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ATGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCGGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAAG ACATCCTACA AGAGCAGCTG GCGGAACCCG TGTATCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATCTATGT GTGCGGGGAT GTCACCATGG CGGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACGCA GAAGGGCAAG CTCTCTGTGG AGGACGCTGG TGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGCTACCAT GAGGATATTT TCGGGGTCAC CCTGCGCACA
    TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAGAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833520.1
    CDS728..5134
    Translation

    Target ORF information:

    RefSeq Version XM_024977752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC GGCCCCTGGG TCCCCCCACA GCAGCCGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ATGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCGGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAAG ACATCCTACA AGAGCAGCTG GCGGAACCCG TGTATCGAGC CCTGAAGGAG
    CAAGGGGGCC ACATCTATGT GTGCGGGGAT GTCACCATGG CGGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACGCA GAAGGGCAAG CTCTCTGTGG AGGACGCTGG TGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGCTACCAT GAGGATATTT TCGGGGTCAC CCTGCGCACA
    TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAGAAA
    GACACCGATG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224634
    Clone ID Related Accession (Same CDS sequence) XM_024977753.1
    Accession Version XM_024977753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC GGCCCCTGGG TCCCCCCACA GCAGCCGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ATGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCGGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGAGCTACAA GGTGCGTTTC AACAGCGTTT CCTCGTACTC CGACTCCCGC
    AAATCATCAG GTGATGGGCC AGACCTCAGA GACAACTTTG AGAGTACTGG ACCCCTGGCC
    AACGTGAGGT TCTCTGTGTT CGGTCTTGGC TCACGGGCAT ACCCTCACTT CTGCGCCTTT
    GGACATGCCG TGGACACTCT CCTGGAAGAG CTTGGAGGAG AGAGGATCTT GAAGATGAGG
    GAGGGGGACG AGCTTTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCTAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTGTT CTGCGTGGGT GATGACGTCA ACATTGAGAA GGCGAACAAC
    TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGTCTCAC CTACGTGGCG
    GAAGCTCCGG AACTCACACA AGGTCTATCC AGTGTCCACA AAAAGCGGGT CTCAGCCGCT
    CGACTCCTCA GCCGCCAGAA CCTTCAGAGT CCAAAATCCA GTCGATCGAC CATCTTCGTG
    CGTCTCCACA CCAATGGGAG TCAGGAGCTG CAGTACCAGC CCGGGGACCA CCTGGGCGTC
    TTCCCCGGCA ACCACGAGGA CCTGGTGAAT GCCCTGATCG AGCGGCTGGA GGATGCCCCC
    CCTGCCAACC AGTTGGTGAA GGTGGAGCTG CTGGAGGAGC GGAACACGGC TTTGGGGGTT
    ATCAGTAACT GGACCGATGA GCATCGCCTG CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACGAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG
    GCCACCAGTG AGAAAGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTCT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAAG TGCTGGAGGA GTTCCCGTCC
    ATCCAGATGC CCTCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC TCGCTACTAT
    TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTCACCGT GGCCATCGTC
    TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCACG GCGTGTGCTC CTCCTGGCTC
    AACCGGATCC AGGCTGACGA AGTGGTGCCC TGTTTCGTGA GAGGAGCGCC CAGCTTCCAC
    CTGCCCCAAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATCGCCCCC
    TTCCGGAGCT TCTGGCAGCA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAGCCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAGGGAGGAG
    ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC AGAGAGCTGT ACACAGCCTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAAGAC ATCCTACAAG AGCAGCTGGC GGAACCCGTG
    TATCGAGCCC TGAAGGAGCA AGGGGGCCAC ATCTATGTGT GCGGGGATGT CACCATGGCG
    GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACGCAGA AGGGCAAGCT CTCTGTGGAG
    GACGCTGGTG TGTTCATCAG CAGGCTGAGG GATGACAACC GCTACCATGA GGATATTTTC
    GGGGTCACCC TGCGCACATA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC
    ATTGAGGAGA GCAAGAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833521.1
    CDS1..4305
    Translation

    Target ORF information:

    RefSeq Version XM_024977753.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC GGCCCCTGGG TCCCCCCACA GCAGCCGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ATGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCGGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGAGCTACAA GGTGCGTTTC AACAGCGTTT CCTCGTACTC CGACTCCCGC
    AAATCATCAG GTGATGGGCC AGACCTCAGA GACAACTTTG AGAGTACTGG ACCCCTGGCC
    AACGTGAGGT TCTCTGTGTT CGGTCTTGGC TCACGGGCAT ACCCTCACTT CTGCGCCTTT
    GGACATGCCG TGGACACTCT CCTGGAAGAG CTTGGAGGAG AGAGGATCTT GAAGATGAGG
    GAGGGGGACG AGCTTTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCTAA GAAGGTCTTC
    AAGGCAGCCT GTGATGTGTT CTGCGTGGGT GATGACGTCA ACATTGAGAA GGCGAACAAC
    TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGTCTCAC CTACGTGGCG
    GAAGCTCCGG AACTCACACA AGGTCTATCC AGTGTCCACA AAAAGCGGGT CTCAGCCGCT
    CGACTCCTCA GCCGCCAGAA CCTTCAGAGT CCAAAATCCA GTCGATCGAC CATCTTCGTG
    CGTCTCCACA CCAATGGGAG TCAGGAGCTG CAGTACCAGC CCGGGGACCA CCTGGGCGTC
    TTCCCCGGCA ACCACGAGGA CCTGGTGAAT GCCCTGATCG AGCGGCTGGA GGATGCCCCC
    CCTGCCAACC AGTTGGTGAA GGTGGAGCTG CTGGAGGAGC GGAACACGGC TTTGGGGGTT
    ATCAGTAACT GGACCGATGA GCATCGCCTG CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ACATCACGAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG
    GCCACCAGTG AGAAAGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTCT GCAGGAGTAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAAG TGCTGGAGGA GTTCCCGTCC
    ATCCAGATGC CCTCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC TCGCTACTAT
    TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTCACCGT GGCCATCGTC
    TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCACG GCGTGTGCTC CTCCTGGCTC
    AACCGGATCC AGGCTGACGA AGTGGTGCCC TGTTTCGTGA GAGGAGCGCC CAGCTTCCAC
    CTGCCCCAAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATCGCCCCC
    TTCCGGAGCT TCTGGCAGCA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAGCCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAGGGAGGAG
    ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC AGAGAGCTGT ACACAGCCTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAAGAC ATCCTACAAG AGCAGCTGGC GGAACCCGTG
    TATCGAGCCC TGAAGGAGCA AGGGGGCCAC ATCTATGTGT GCGGGGATGT CACCATGGCG
    GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACGCAGA AGGGCAAGCT CTCTGTGGAG
    GACGCTGGTG TGTTCATCAG CAGGCTGAGG GATGACAACC GCTACCATGA GGATATTTTC
    GGGGTCACCC TGCGCACATA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC
    ATTGAGGAGA GCAAGAAAGA CACCGATGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93237
    Clone ID Related Accession (Same CDS sequence) XM_015467111.1 , XM_015475535.1
    Accession Version XM_015467111.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, brain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC AGCCCCTGGG TCCCCCCACA GCAGCTGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ACGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCAGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGGTA
    AGCCCCAGCC CGTCACACAC GTGTGCATGC ACACTCTGCT CCCGAACACC CTCACCCCTT
    CTGAACTCTC CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322597.1
    CDS911..5068
    Translation

    Target ORF information:

    RefSeq Version XM_015467111.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 1 (NOS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467111.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC AGCCCCTGGG TCCCCCCACA GCAGCTGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ACGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCAGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGGTA
    AGCCCCAGCC CGTCACACAC GTGTGCATGC ACACTCTGCT CCCGAACACC CTCACCCCTT
    CTGAACTCTC CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93237
    Clone ID Related Accession (Same CDS sequence) XM_015467111.1 , XM_015475535.1
    Accession Version XM_015475535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, brain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC AGCCCCTGGG TCCCCCCACA GCAGCTGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ACGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCAGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGGTA
    AGCCCCAGCC CGTCACACAC GTGTGCATGC ACACTCTGCT CCCGAACACC CTCACCCCTT
    CTGAACTCTC CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331021.1
    CDS911..5068
    Translation

    Target ORF information:

    RefSeq Version XM_015475535.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 1 (NOS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGAGCC ACATGTTCAG CGTTCAACAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCC
    GTGATCATCT CTGACCTGAT CCGAGGGGGC GCCGCGGAGC AAAGCGGCCT CATCCAGGCT
    GGGGACATCA TTCTCGCCGT CAATGGCCAG CCCTTGGTGG ACCTGAGCTA CGACAGCGCC
    CTGGAGGTGC TCAGGGGGAT TGCCTCTGAG ACCCACGTGG TGCTCATCCT CAGGGGCCCC
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ACGGGACCCC CAAGACCATC
    CGGGTGACCC AGCCCCTGGG TCCCCCCACA GCAGCTGTTG ACCTATCCCA CCAAGCGTCA
    GCCGGCAGAG AGCAGACGCT GGTGGTGGAC GGGGCCACAG GGCCCGGCAA TGGGCCCCAG
    CATGCGCCAG ACCCGGGGCA GGAAGCCAGC TCCCTCGCCC ACGCCAACGG CCTGGCTGCC
    AGGCCCCCAG GCCAGGACCC AGCCAAGAAA AGCACTGGGG TCGCCCTCCA GGGCAGCAGA
    GAGAACAATA AACTGCTCAA AGAGATAGAG CCTGTCCTGA ACCTTCTCAC CAGTGGGGGC
    AAAGCGATCA ATGGAGGGGG ACCTGCCAAG ACAGAGACCA AGGACGTGGA AATCCAGGTG
    GACAGAGACC TGGACGGCAA ATCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAGTG ACCTGTGGGG GAAGGGCAGC ATGCCCGTCG TCCTCAACAA CCCGTACTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCTC GCTTCCTCAA GGTCAAGAAC TGGGAGACCG ACGTGGTTCT CTCTGACACC
    CTCCACCTCA AGAGCACGTT GGGAACGGGA TGCACTGAGC ACATCTGTAT GGGGTCCATC
    ATGCTCCCTT CTCAGCAAAT ACGAAAGCCT GAAGACGTCC GCACAAAAGA ACAGCTCTTT
    CCCCTTGCCA AAGAGTTCAT TGATCAGTAC TACTCATCAA TTAAAAGGTT TGGCTCCAAA
    GCCCACATGG AGAGGCTGGA AGAGGTGAAC AAAGAAATCG AAACCACCAG CACCTACCAG
    CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGT
    GTTGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTTGATG CCCGAGACTG TACCACTGCT
    CATGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGATCTGCGA TCACCATATT TCCCCAGAGG ACCGACGGCA AGCACGACTT CCGCGTTTGG
    AACTCCCAAC TCATCCGCTA CGCTGGCTAC AAGCAGCCCG ACGGCTCCAT CCTGGGGGAC
    CCAGCCAACG TGGAGTTCAC GGAGATCTGC ATACAGCAGG GCTGGAAACC CCCACGAAGC
    CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCAAACGGCA ATGACCCAGA GCTCTTCCAG
    ATCCCTCCAG AGTTGGTGCT GGAAGTCCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTCCT GGAGATCGGC
    GGCCTGGAGT TCAGCGCGTG TCCCTTCAGC GGCTGGTACA TGGGCACAGA GATTGGCGTC
    CGCGACTACT GCGACAGCTC CCGATACAAC ATCCTGGAGG ATGTGGCCAA GAAGATGAAC
    TTGGACATGA GGAAGACATC CTCTCTGTGG AAGGACCAGG CACTGGTGGA GATCAATATT
    GCGGTTCTGT ACAGCTTCCA GAGTGACAAG GTGACCATCG TCGACCACCA CTCCGCCACC
    GAGTCCTTCA TTAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TCGTGCCCCC CATGTCTGGC AGTATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACACC CTCCTTCGAA TACCAGCCTG ACCCTTGGAA CACCCATGTC
    TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCAGC CAAACTGATG GGGCAGGCTA TGGCCAAGAG GGTCAAGGCT
    ACCATCCTTT ATGCCACAGA GACGGGCAAG TCGCAGGCTT ATGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ATGCTTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTACAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT
    GAGAATGGGG AGAAATTTGG CTGTGCTCTG ATGGAAATGA GGAACCCTAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCTCC
    CGTGGTGACA GAGAAGTCCA CGGTCTGGCT GCAGTTCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTGCGTT TCAACAGCGT TTCCTCGTAC TCCGACTCCC GCAAATCATC AGGTGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACT GGACCCCTGG CCAACGTGAG GTTCTCTGTG
    TTCGGTCTTG GCTCACGGGC ATACCCTCAC TTCTGCGCCT TTGGACATGC CGTGGACACT
    CTCCTGGAAG AGCTTGGAGG AGAGAGGATC TTGAAGATGA GGGAGGGGGA CGAGCTTTGT
    GGGCAGGAAG AGGCTTTCAG GACCTGGGCT AAGAAGGTCT TCAAGGCAGC CTGTGATGTG
    TTCTGCGTGG GTGATGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGTCTC ACCTACGTGG CGGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAGTGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAG
    AACCTTCAGA GTCCAAAATC CAGTCGATCG ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AGTCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTGGTGA ATGCCCTGAT CGAGCGGCTG GAGGATGCCC CCCCTGCCAA CCAGTTGGTG
    AAGGTGGAGC TGCTGGAGGA GCGGAACACG GCTTTGGGGG TTATCAGTAA CTGGACCGAT
    GAGCATCGCC TGCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACG
    ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA AGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCTCCACC
    CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCTCGCTACT ATTCCATCAG CTCCTCCCCG
    GACATGTACC CCGACGAGGT GCACCTCACC GTGGCCATCG TCTCCTACCG CACCCGAGAT
    GGAGAAGGAC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC
    GAAGTGGTGC CCTGTTTCGT GAGAGGAGCG CCCAGCTTCC ACCTGCCCCA AAATCCCCAA
    GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATCGCCC CCTTCCGGAG CTTCTGGCAG
    CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAGCCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAGGGAGG AGACCCTGCA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAGCT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGGTA
    AGCCCCAGCC CGTCACACAC GTGTGCATGC ACACTCTGCT CCCGAACACC CTCACCCCTT
    CTGAACTCTC CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.