RIMS2 cDNA ORF clone, Bos taurus(cattle)

The following RIMS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIMS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa41163 NM_001102190.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa53224 XM_010812124.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa53225 XM_010812125.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa53227 XM_010812127.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa53230 XM_010812130.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa222376 XM_025001671.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa222366 XM_025001660.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222367 XM_025001662.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222368 XM_025001663.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222369 XM_025001664.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222370 XM_025001665.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222371 XM_025001666.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222372 XM_025001667.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222373 XM_025001668.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222374 XM_025001669.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222375 XM_025001670.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222377 XM_025001672.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222378 XM_025001673.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222379 XM_025001674.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222380 XM_025001675.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa92550 XM_015474495.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92551 XM_015474496.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92552 XM_015474497.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa26046 XM_005215574.4
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26047 XM_005215575.4
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa222361 XM_025001653.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa222362 XM_025001654.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa222363 XM_025001656.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa222364 XM_025001657.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222365 XM_025001658.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa92546 XM_015474491.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92547 XM_015474492.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92548 XM_015474493.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92549 XM_015474494.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa26045 XM_005215573.3
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa53231 XM_025001659.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa53232 XM_025001661.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa26046 XM_005215574.3 Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26047 XM_005215575.3 Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa41163
    Clone ID Related Accession (Same CDS sequence) NM_001102190.1
    Accession Version NM_001102190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151717.1. On or before Aug 21, 2007 this sequence version replaced XM_001250297.1, XM_001250352.1, XM_001250393.1, XM_615784.3, XM_001250489.1, XM_001250533.1. ##Evidence-Data-START## Transcript exon combination :: BC151717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCCGGC AGGGCTTAGG GGGCGCCGGC GCCGCGGGGC GCTCCATGCA GCGCTCGCAG 
    AGCCGGAGCA GCCTCTCCGC CTCCTTCGAG GCACTGGCCG GCTACTTTCC CTGTATGAAC
    TCCCTGGAGG AAGAAGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095660.1
    CDS325..1176
    Translation

    Target ORF information:

    RefSeq Version NM_001102190.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCCGGC AGGGCTTAGG GGGCGCCGGC GCCGCGGGGC GCTCCATGCA GCGCTCGCAG 
    AGCCGGAGCA GCCTCTCCGC CTCCTTCGAG GCACTGGCCG GCTACTTTCC CTGTATGAAC
    TCCCTGGAGG AAGAAGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53224
    Clone ID Related Accession (Same CDS sequence) XM_010812124.1
    Accession Version XM_010812124.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCATTA TTCTCCAGAC AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA 
    GTCAGAGCGT TGAACATCAT CACAGGGATG GCAGGTCTCA TCCTCGTACT GGGTCTGTCC
    AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC
    CACCAAAGGG AACATTGGAG AGAAAAGACG TGGACGCCAC AAGGAGAAGA CATGCAGTAT
    CGATCGGGAT GGGATCCTCA TAGAGGGGCA GATAATATTT CCACTAAATC TTCGGACAGT
    GACGTAAGTG ATATATCTGC GGTTTCCAGG ACTAGTAGTG CTTCTCGTTT CAGCAGCACA
    AGCTACATGT CTGTCCAATC AGAACGGCCA AGAGGAAACA AGAAAATCAG GCCAAAGGGA
    CTAGAAGAGG GAGGGAAAGA CGGGGATAAG CATGAAGAGA TAGTTCATGC GGAGGAAGAG
    GTAAAGGAAG AAAGAACTAA TGAGAATGAG AAAGGGAAAG ACATTACAAA CATGTGTAAT
    AAGGAGACAA ACAGAGAATC AGGAGATGAG GAAAAAACAC CAGATATACC TGAGCAAGGG
    AAAGAAAAAG AACAGTGGAA TAAAGAGGAT TTGCAAAAGC AATTCTCACA AGATGATGAC
    AGTGTCTTTA CATCCAAAAT GCAAAGCAGA CAAATGGGCA TCTCAGGGAA GAACATGACC
    AAGAGCACCA GCATCAGCGG AGACATGTGC TCGCTGGAGA AGAATGATGG CAGCCAGTCG
    GACACCGCAG TGGGTGCCTT GGGCACCAGC AGCAAGAAGC GACGCTCTAG CATTGGGGCC
    AAAATGGTAG CTATCGTTGG TCTTTCCCGG AAGAGCCGCA GTGCTTCTCA GCTCAGCCAA
    ACGGAAACCG GAGGTAAAAA GCTACGGAGC ACTGTCCAGA GAAGTACAGA AACAGGCTTG
    GCTGTGGAAA TGAGGAACTG GATGACTCGA CAAGCGAGCC GAGAATCTAC CGACGGCAGC
    ATGAACAGCT ACAGCTCAGA AGGAAATCTG ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT
    AGCCAATTCA GTGATTTTCT GGATGGCCTT GGCCCTGCCC AGCTAGTGGG GCGCCAAACT
    CTGGCCACAC CCGCCATGGG TGACATTCAG GTAGGAATGA TGGACAAAAA GGGACAGCTG
    GAGGTGGAAA TAATTCGGGC CCGTGGCCTT GTTGTAAAAC CAGGTTCCAA GACACTGCCA
    GCACCATACG TAAAGGTGTA TCTATTAGAT AATGGAGTCT GCATAGCCAA AAAGAAAACA
    AAAGTGGCAA GAAAAACGCT GGAACCCCTT TACCAGCAAC TATTATCTTT TGAAGAAAGT
    CCCCAAGGAA AGGTTTTACA GATCATTGTC TGGGGAGACT ATGGCCGCAT GGATCACAAA
    TCCTTTATGG GAGTGGCCCA GATACTTTTA GATGAACTGG AGCTATCCAA CATGGTGATC
    GGCTGGTTCA AACTCTTCCC CCCTTCCTCC CTAGTAGATC CAACCTTGGC CCCTCTGACA
    AGAAGAGCTT CCCAATCATC TCTGGAAAGT TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810426.1
    CDS83..1702
    Translation

    Target ORF information:

    RefSeq Version XM_010812124.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACCATTA TTCTCCAGAC AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA 
    GTCAGAGCGT TGAACATCAT CACAGGGATG GCAGGTCTCA TCCTCGTACT GGGTCTGTCC
    AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC
    CACCAAAGGG AACATTGGAG AGAAAAGACG TGGACGCCAC AAGGAGAAGA CATGCAGTAT
    CGATCGGGAT GGGATCCTCA TAGAGGGGCA GATAATATTT CCACTAAATC TTCGGACAGT
    GACGTAAGTG ATATATCTGC GGTTTCCAGG ACTAGTAGTG CTTCTCGTTT CAGCAGCACA
    AGCTACATGT CTGTCCAATC AGAACGGCCA AGAGGAAACA AGAAAATCAG GCCAAAGGGA
    CTAGAAGAGG GAGGGAAAGA CGGGGATAAG CATGAAGAGA TAGTTCATGC GGAGGAAGAG
    GTAAAGGAAG AAAGAACTAA TGAGAATGAG AAAGGGAAAG ACATTACAAA CATGTGTAAT
    AAGGAGACAA ACAGAGAATC AGGAGATGAG GAAAAAACAC CAGATATACC TGAGCAAGGG
    AAAGAAAAAG AACAGTGGAA TAAAGAGGAT TTGCAAAAGC AATTCTCACA AGATGATGAC
    AGTGTCTTTA CATCCAAAAT GCAAAGCAGA CAAATGGGCA TCTCAGGGAA GAACATGACC
    AAGAGCACCA GCATCAGCGG AGACATGTGC TCGCTGGAGA AGAATGATGG CAGCCAGTCG
    GACACCGCAG TGGGTGCCTT GGGCACCAGC AGCAAGAAGC GACGCTCTAG CATTGGGGCC
    AAAATGGTAG CTATCGTTGG TCTTTCCCGG AAGAGCCGCA GTGCTTCTCA GCTCAGCCAA
    ACGGAAACCG GAGGTAAAAA GCTACGGAGC ACTGTCCAGA GAAGTACAGA AACAGGCTTG
    GCTGTGGAAA TGAGGAACTG GATGACTCGA CAAGCGAGCC GAGAATCTAC CGACGGCAGC
    ATGAACAGCT ACAGCTCAGA AGGAAATCTG ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT
    AGCCAATTCA GTGATTTTCT GGATGGCCTT GGCCCTGCCC AGCTAGTGGG GCGCCAAACT
    CTGGCCACAC CCGCCATGGG TGACATTCAG GTAGGAATGA TGGACAAAAA GGGACAGCTG
    GAGGTGGAAA TAATTCGGGC CCGTGGCCTT GTTGTAAAAC CAGGTTCCAA GACACTGCCA
    GCACCATACG TAAAGGTGTA TCTATTAGAT AATGGAGTCT GCATAGCCAA AAAGAAAACA
    AAAGTGGCAA GAAAAACGCT GGAACCCCTT TACCAGCAAC TATTATCTTT TGAAGAAAGT
    CCCCAAGGAA AGGTTTTACA GATCATTGTC TGGGGAGACT ATGGCCGCAT GGATCACAAA
    TCCTTTATGG GAGTGGCCCA GATACTTTTA GATGAACTGG AGCTATCCAA CATGGTGATC
    GGCTGGTTCA AACTCTTCCC CCCTTCCTCC CTAGTAGATC CAACCTTGGC CCCTCTGACA
    AGAAGAGCTT CCCAATCATC TCTGGAAAGT TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53225
    Clone ID Related Accession (Same CDS sequence) XM_010812125.1
    Accession Version XM_010812125.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACCATTA TTCTCCAGAC AGAGACAGGT CTCATCCTCG TACTGGGTCT GTCCAAACAA 
    GCCCATCAAG TACTCCAGTG GCAGGACGAA GGGGCCGACA ACTCCCACAG CTTCCACCAA
    AGGGAACATT GGAGAGAAAA GACGTGGACG CCACAAGGAG AAGACATGCA GTATCGATCG
    GGATGGGATC CTCATAGAGG GGCAGATAAT ATTTCCACTA AATCTTCGGA CAGTGACGTA
    AGTGATATAT CTGCGGTTTC CAGGACTAGT AGTGCTTCTC GTTTCAGCAG CACAAGCTAC
    ATGTCTGTCC AATCAGAACG GCCAAGAGGA AACAAGAAAA TCAGGCCAAA GGGACTAGAA
    GAGGGAGGGA AAGACGGGGA TAAGCATGAA GAGATAGTTC ATGCGGAGGA AGAGGTAAAG
    GAAGAAAGAA CTAATGAGAA TGAGAAAGGG AAAGACATTA CAAACATGTG TAATAAGGAG
    ACAAACAGAG AATCAGGAGA TGAGGAAAAA ACACCAGATA TACCTGAGCA AGGGAAAGAA
    AAAGAACAGT GGAATAAAGA GGATTTGCAA AAGCAATTCT CACAAGATGA TGACAGTGTC
    TTTACATCCA AAATGCAAAG CAGACAAATG GGCATCTCAG GGAAGAACAT GACCAAGAGC
    ACCAGCATCA GCGGAGACAT GTGCTCGCTG GAGAAGAATG ATGGCAGCCA GTCGGACACC
    GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG AAGCGACGCT CTAGCATTGG GGCCAAAATG
    GTAGCTATCG TTGGTCTTTC CCGGAAGAGC CGCAGTGCTT CTCAGCTCAG CCAAACGGAA
    ACCGGAGGTA AAAAGCTACG GAGCACTGTC CAGAGAAGTA CAGAAACAGG CTTGGCTGTG
    GAAATGAGGA ACTGGATGAC TCGACAAGCG AGCCGAGAAT CTACCGACGG CAGCATGAAC
    AGCTACAGCT CAGAAGGAAA TCTGATCTTC CCTGGTGTCC GCTTGGCCTC GGATAGCCAA
    TTCAGTGATT TTCTGGATGG CCTTGGCCCT GCCCAGCTAG TGGGGCGCCA AACTCTGGCC
    ACACCCGCCA TGGGTGACAT TCAGGTAGGA ATGATGGACA AAAAGGGACA GCTGGAGGTG
    GAAATAATTC GGGCCCGTGG CCTTGTTGTA AAACCAGGTT CCAAGACACT GCCAGCACCA
    TACGTAAAGG TGTATCTATT AGATAATGGA GTCTGCATAG CCAAAAAGAA AACAAAAGTG
    GCAAGAAAAA CGCTGGAACC CCTTTACCAG CAACTATTAT CTTTTGAAGA AAGTCCCCAA
    GGAAAGGTTT TACAGATCAT TGTCTGGGGA GACTATGGCC GCATGGATCA CAAATCCTTT
    ATGGGAGTGG CCCAGATACT TTTAGATGAA CTGGAGCTAT CCAACATGGT GATCGGCTGG
    TTCAAACTCT TCCCCCCTTC CTCCCTAGTA GATCCAACCT TGGCCCCTCT GACAAGAAGA
    GCTTCCCAAT CATCTCTGGA AAGTTCAACT GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810427.1
    CDS83..1636
    Translation

    Target ORF information:

    RefSeq Version XM_010812125.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACCATTA TTCTCCAGAC AGAGACAGGT CTCATCCTCG TACTGGGTCT GTCCAAACAA 
    GCCCATCAAG TACTCCAGTG GCAGGACGAA GGGGCCGACA ACTCCCACAG CTTCCACCAA
    AGGGAACATT GGAGAGAAAA GACGTGGACG CCACAAGGAG AAGACATGCA GTATCGATCG
    GGATGGGATC CTCATAGAGG GGCAGATAAT ATTTCCACTA AATCTTCGGA CAGTGACGTA
    AGTGATATAT CTGCGGTTTC CAGGACTAGT AGTGCTTCTC GTTTCAGCAG CACAAGCTAC
    ATGTCTGTCC AATCAGAACG GCCAAGAGGA AACAAGAAAA TCAGGCCAAA GGGACTAGAA
    GAGGGAGGGA AAGACGGGGA TAAGCATGAA GAGATAGTTC ATGCGGAGGA AGAGGTAAAG
    GAAGAAAGAA CTAATGAGAA TGAGAAAGGG AAAGACATTA CAAACATGTG TAATAAGGAG
    ACAAACAGAG AATCAGGAGA TGAGGAAAAA ACACCAGATA TACCTGAGCA AGGGAAAGAA
    AAAGAACAGT GGAATAAAGA GGATTTGCAA AAGCAATTCT CACAAGATGA TGACAGTGTC
    TTTACATCCA AAATGCAAAG CAGACAAATG GGCATCTCAG GGAAGAACAT GACCAAGAGC
    ACCAGCATCA GCGGAGACAT GTGCTCGCTG GAGAAGAATG ATGGCAGCCA GTCGGACACC
    GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG AAGCGACGCT CTAGCATTGG GGCCAAAATG
    GTAGCTATCG TTGGTCTTTC CCGGAAGAGC CGCAGTGCTT CTCAGCTCAG CCAAACGGAA
    ACCGGAGGTA AAAAGCTACG GAGCACTGTC CAGAGAAGTA CAGAAACAGG CTTGGCTGTG
    GAAATGAGGA ACTGGATGAC TCGACAAGCG AGCCGAGAAT CTACCGACGG CAGCATGAAC
    AGCTACAGCT CAGAAGGAAA TCTGATCTTC CCTGGTGTCC GCTTGGCCTC GGATAGCCAA
    TTCAGTGATT TTCTGGATGG CCTTGGCCCT GCCCAGCTAG TGGGGCGCCA AACTCTGGCC
    ACACCCGCCA TGGGTGACAT TCAGGTAGGA ATGATGGACA AAAAGGGACA GCTGGAGGTG
    GAAATAATTC GGGCCCGTGG CCTTGTTGTA AAACCAGGTT CCAAGACACT GCCAGCACCA
    TACGTAAAGG TGTATCTATT AGATAATGGA GTCTGCATAG CCAAAAAGAA AACAAAAGTG
    GCAAGAAAAA CGCTGGAACC CCTTTACCAG CAACTATTAT CTTTTGAAGA AAGTCCCCAA
    GGAAAGGTTT TACAGATCAT TGTCTGGGGA GACTATGGCC GCATGGATCA CAAATCCTTT
    ATGGGAGTGG CCCAGATACT TTTAGATGAA CTGGAGCTAT CCAACATGGT GATCGGCTGG
    TTCAAACTCT TCCCCCCTTC CTCCCTAGTA GATCCAACCT TGGCCCCTCT GACAAGAAGA
    GCTTCCCAAT CATCTCTGGA AAGTTCAACT GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53227
    Clone ID Related Accession (Same CDS sequence) XM_010812127.1
    Accession Version XM_010812127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGAAGA CGTGGACGCC ACAAGGAGAA GACATGCAGT ATCGATCGGG ATGGGATCCT 
    CATAGAGGGG CAGATAATAT TTCCACTAAA TCTTCGGACA GTGACGTAAG TGATATATCT
    GCGGTTTCCA GGACTAGTAG TGCTTCTCGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAA
    TCAGAACGGC CAAGAGGAAA CAAGAAAATC AGGCCAAAGG GACTAGAAGA GGGAGGGAAA
    GACGGGGATA AGCATGAAGA GATAGTTCAT GCGGAGGAAG AGGTAAAGGA AGAAAGAACT
    AATGAGAATG AGAAAGGGAA AGACATTACA AACATGTGTA ATAAGGAGAC AAACAGAGAA
    TCAGGAGATG AGGAAAAAAC ACCAGATATA CCTGAGCAAG GGAAAGAAAA AGAACAGTGG
    AATAAAGAGG ATTTGCAAAA GCAATTCTCA CAAGATGATG ACAGTGTCTT TACATCCAAA
    ATGCAAAGCA GACAAATGGG CATCTCAGGG AAGAACATGA CCAAGAGCAC CAGCATCAGC
    GGAGACATGT GCTCGCTGGA GAAGAATGAT GGCAGCCAGT CGGACACCGC AGTGGGTGCC
    TTGGGCACCA GCAGCAAGAA GCGACGCTCT AGCATTGGGG CCAAAATGGT AGCTATCGTT
    GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT CAGCTCAGCC AAACGGAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810429.1
    CDS28..1449
    Translation

    Target ORF information:

    RefSeq Version XM_010812127.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGAAGA CGTGGACGCC ACAAGGAGAA GACATGCAGT ATCGATCGGG ATGGGATCCT 
    CATAGAGGGG CAGATAATAT TTCCACTAAA TCTTCGGACA GTGACGTAAG TGATATATCT
    GCGGTTTCCA GGACTAGTAG TGCTTCTCGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAA
    TCAGAACGGC CAAGAGGAAA CAAGAAAATC AGGCCAAAGG GACTAGAAGA GGGAGGGAAA
    GACGGGGATA AGCATGAAGA GATAGTTCAT GCGGAGGAAG AGGTAAAGGA AGAAAGAACT
    AATGAGAATG AGAAAGGGAA AGACATTACA AACATGTGTA ATAAGGAGAC AAACAGAGAA
    TCAGGAGATG AGGAAAAAAC ACCAGATATA CCTGAGCAAG GGAAAGAAAA AGAACAGTGG
    AATAAAGAGG ATTTGCAAAA GCAATTCTCA CAAGATGATG ACAGTGTCTT TACATCCAAA
    ATGCAAAGCA GACAAATGGG CATCTCAGGG AAGAACATGA CCAAGAGCAC CAGCATCAGC
    GGAGACATGT GCTCGCTGGA GAAGAATGAT GGCAGCCAGT CGGACACCGC AGTGGGTGCC
    TTGGGCACCA GCAGCAAGAA GCGACGCTCT AGCATTGGGG CCAAAATGGT AGCTATCGTT
    GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT CAGCTCAGCC AAACGGAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53230
    Clone ID Related Accession (Same CDS sequence) XM_010812130.2
    Accession Version XM_010812130.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCAAAGCA GACAAATGGG CATCTCAGGG AAGAACATGA CCAAGAGCAC CAGCATCAGC 
    GGAGACATGT GCTCGCTGGA GAAGAATGAT GGCAGCCAGT CGGACACCGC AGTGGGTGCC
    TTGGGCACCA GCAGCAAGAA GCGACGCTCT AGCATTGGGG CCAAAATGGT AGCTATCGTT
    GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT CAGCTCAGCC AAACGGAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810432.1
    CDS151..1092
    Translation

    Target ORF information:

    RefSeq Version XM_010812130.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCAAAGCA GACAAATGGG CATCTCAGGG AAGAACATGA CCAAGAGCAC CAGCATCAGC 
    GGAGACATGT GCTCGCTGGA GAAGAATGAT GGCAGCCAGT CGGACACCGC AGTGGGTGCC
    TTGGGCACCA GCAGCAAGAA GCGACGCTCT AGCATTGGGG CCAAAATGGT AGCTATCGTT
    GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT CAGCTCAGCC AAACGGAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222376
    Clone ID Related Accession (Same CDS sequence) XM_025001671.1
    Accession Version XM_025001671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA 
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGG CCAAAGGGAC TAGAAGAGGG AGGGAAAGAC
    GGGGATAAGC ATGAAGAGAT AGTTCATGCG GAGGAAGAGG TAAAGGAAGA AAGAACTAAT
    GAGAATGAGA AAGGGAAAAA CATTACAAAC ATGTGTAATA AGGAGACAAA CAGAGAATCA
    GGAGATGAGG AAAAAACACC AGATATACCT GAGCAAGGGA AAGAAAAAGA ACAGTGGAAT
    AAAGAGGATT TGCAAAAGCA ATTCTCACAA GATGATGACA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857439.1
    CDS6250..7728
    Translation

    Target ORF information:

    RefSeq Version XM_025001671.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA 
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGG CCAAAGGGAC TAGAAGAGGG AGGGAAAGAC
    GGGGATAAGC ATGAAGAGAT AGTTCATGCG GAGGAAGAGG TAAAGGAAGA AAGAACTAAT
    GAGAATGAGA AAGGGAAAAA CATTACAAAC ATGTGTAATA AGGAGACAAA CAGAGAATCA
    GGAGATGAGG AAAAAACACC AGATATACCT GAGCAAGGGA AAGAAAAAGA ACAGTGGAAT
    AAAGAGGATT TGCAAAAGCA ATTCTCACAA GATGATGACA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222366
    Clone ID Related Accession (Same CDS sequence) XM_025001660.1
    Accession Version XM_025001660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857428.1
    CDS373..4683
    Translation

    Target ORF information:

    RefSeq Version XM_025001660.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222367
    Clone ID Related Accession (Same CDS sequence) XM_025001662.1
    Accession Version XM_025001662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAAGCC AAAGCCTTAG TAGAAGAACA
    ACGCCTTTTG TTCCTAGGGT TCAGATAAAA CTGTGGTTTG ACAAGGTTGG TCACCAATTA
    ATAGTTACAA TTTTGGGAGC AAAAGACCTC CCTTCCAGGG AAGATGGGAG GCCAAGGAAT
    CCTTATGTTA AAATTTACTT TCTTCCAGAC AGAAGTGACA AAAACAAGAG AAGAACTAAA
    ACAGTGAAGA AAACTTTGGA ACCCAAATGG AACCAAACAT TCATTTATTC TCCAGTCCAC
    CGAAGAGAGT TTCGGGAACG GATGCTGGAG ATTACCCTTT GGGATCAAGC TCGAGTTCGA
    GAGGAAGAAA GTGAATTTTT AGGAGAGATT TTAATTGAAT TAGAAACAGC ATTATTAGAT
    GATGAGCCAC ATTGGTACAA ACTTCAGACT CACGATGTTT CTTCATTGCC ACTTCCCCAT
    CCTTCACCAT ATATGCCACG ACGACAACTC CATGGAGAGA GCCCAACACG GAGGTTGCAA
    AGATCAAAGA GAATAAGTGA TAGTGAGGTC TCTGATTATG ACTGTGATGA TGGAATTGGT
    GTAGTATCAG ATTATCGACA CAATGGTCGG GATCTTCAAA GCTCAACATT GTCAGTGCCA
    GAACAAGTAA TGTCATCCAA CCACTGTTCG CCGTCAGGGT CTCCACACCG AGTAGATGTT
    ATAGGAAGGA CTAGATCTTG GTCACCCAGT GTCCCTCCTC CACAGAGTCG AAATGTGGAG
    CAGGGACTTC GAGGGACACG CTCTGCTATA GGACATTATA ATACAATTAG CCGAATGGAT
    AGACACCGTG TCATGGATGA CCATTATTCT CCAGACAGAG ACAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAAAACCG GAGGTAAAAA GCTACGGAGC
    ACTGTCCAGA GAAGTACAGA AACAGGCTTG GCTGTGGAAA TGAGGAACTG GATGACTCGA
    CAAGCGAGCC GAGAATCTAC CGACGGCAGC ATGAACAGCT ACAGCTCAGA AGGAAATCTG
    ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT AGCCAATTCA GTGATTTTCT GGATGGCCTT
    GGCCCTGCCC AGCTAGTGGG GCGCCAAACT CTGGCCACAC CCGCCATGGG TGACATTCAG
    GTAGGAATGA TGGACAAAAA GGGACAGCTG GAGGTGGAAA TAATTCGGGC CCGTGGCCTT
    GTTGTAAAAC CAGGTTCCAA GACACTGCCA GCACCATACG TAAAGGTGTA TCTATTAGAT
    AATGGAGTCT GCATAGCCAA AAAGAAAACA AAAGTGGCAA GAAAAACGCT GGAACCCCTT
    TACCAGCAAC TATTATCTTT TGAAGAAAGT CCCCAAGGAA AGGTTTTACA GATCATTGTC
    TGGGGAGACT ATGGCCGCAT GGATCACAAA TCCTTTATGG GAGTGGCCCA GATACTTTTA
    GATGAACTGG AGCTATCCAA CATGGTGATC GGCTGGTTCA AACTCTTCCC CCCTTCCTCC
    CTAGTAGATC CAACCTTGGC CCCTCTGACA AGAAGAGCTT CCCAATCATC TCTGGAAAGT
    TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857430.1
    CDS373..4542
    Translation

    Target ORF information:

    RefSeq Version XM_025001662.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAAGCC AAAGCCTTAG TAGAAGAACA
    ACGCCTTTTG TTCCTAGGGT TCAGATAAAA CTGTGGTTTG ACAAGGTTGG TCACCAATTA
    ATAGTTACAA TTTTGGGAGC AAAAGACCTC CCTTCCAGGG AAGATGGGAG GCCAAGGAAT
    CCTTATGTTA AAATTTACTT TCTTCCAGAC AGAAGTGACA AAAACAAGAG AAGAACTAAA
    ACAGTGAAGA AAACTTTGGA ACCCAAATGG AACCAAACAT TCATTTATTC TCCAGTCCAC
    CGAAGAGAGT TTCGGGAACG GATGCTGGAG ATTACCCTTT GGGATCAAGC TCGAGTTCGA
    GAGGAAGAAA GTGAATTTTT AGGAGAGATT TTAATTGAAT TAGAAACAGC ATTATTAGAT
    GATGAGCCAC ATTGGTACAA ACTTCAGACT CACGATGTTT CTTCATTGCC ACTTCCCCAT
    CCTTCACCAT ATATGCCACG ACGACAACTC CATGGAGAGA GCCCAACACG GAGGTTGCAA
    AGATCAAAGA GAATAAGTGA TAGTGAGGTC TCTGATTATG ACTGTGATGA TGGAATTGGT
    GTAGTATCAG ATTATCGACA CAATGGTCGG GATCTTCAAA GCTCAACATT GTCAGTGCCA
    GAACAAGTAA TGTCATCCAA CCACTGTTCG CCGTCAGGGT CTCCACACCG AGTAGATGTT
    ATAGGAAGGA CTAGATCTTG GTCACCCAGT GTCCCTCCTC CACAGAGTCG AAATGTGGAG
    CAGGGACTTC GAGGGACACG CTCTGCTATA GGACATTATA ATACAATTAG CCGAATGGAT
    AGACACCGTG TCATGGATGA CCATTATTCT CCAGACAGAG ACAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAAAACCG GAGGTAAAAA GCTACGGAGC
    ACTGTCCAGA GAAGTACAGA AACAGGCTTG GCTGTGGAAA TGAGGAACTG GATGACTCGA
    CAAGCGAGCC GAGAATCTAC CGACGGCAGC ATGAACAGCT ACAGCTCAGA AGGAAATCTG
    ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT AGCCAATTCA GTGATTTTCT GGATGGCCTT
    GGCCCTGCCC AGCTAGTGGG GCGCCAAACT CTGGCCACAC CCGCCATGGG TGACATTCAG
    GTAGGAATGA TGGACAAAAA GGGACAGCTG GAGGTGGAAA TAATTCGGGC CCGTGGCCTT
    GTTGTAAAAC CAGGTTCCAA GACACTGCCA GCACCATACG TAAAGGTGTA TCTATTAGAT
    AATGGAGTCT GCATAGCCAA AAAGAAAACA AAAGTGGCAA GAAAAACGCT GGAACCCCTT
    TACCAGCAAC TATTATCTTT TGAAGAAAGT CCCCAAGGAA AGGTTTTACA GATCATTGTC
    TGGGGAGACT ATGGCCGCAT GGATCACAAA TCCTTTATGG GAGTGGCCCA GATACTTTTA
    GATGAACTGG AGCTATCCAA CATGGTGATC GGCTGGTTCA AACTCTTCCC CCCTTCCTCC
    CTAGTAGATC CAACCTTGGC CCCTCTGACA AGAAGAGCTT CCCAATCATC TCTGGAAAGT
    TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222368
    Clone ID Related Accession (Same CDS sequence) XM_025001663.1
    Accession Version XM_025001663.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAATAA AACTGTGGTT TGACAAGGTT
    GGTCACCAAT TAATAGTTAC AATTTTGGGA GCAAAAGACC TCCCTTCCAG GGAAGATGGG
    AGGCCAAGGA ATCCTTATGT TAAAATTTAC TTTCTTCCAG ACAGAAGTGA CAAAAACAAG
    AGAAGAACTA AAACAGTGAA GAAAACTTTG GAACCCAAAT GGAACCAAAC ATTCATTTAT
    TCTCCAGTCC ACCGAAGAGA GTTTCGGGAA CGGATGCTGG AGATTACCCT TTGGGATCAA
    GCTCGAGTTC GAGAGGAAGA AAGTGAATTT TTAGGAGAGA TTTTAATTGA ATTAGAAACA
    GCATTATTAG ATGATGAGCC ACATTGGTAC AAACTTCAGA CTCACGATGT TTCTTCATTG
    CCACTTCCCC ATCCTTCACC ATATATGCCA CGACGACAAC TCCATGGAGA GAGCCCAACA
    CGGAGGTTGC AAAGATCAAA GAGAATAAGT GATAGTGAGG TCTCTGATTA TGACTGTGAT
    GATGGAATTG GTGTAGTATC AGATTATCGA CACAATGGTC GGGATCTTCA AAGCTCAACA
    TTGTCAGTGC CAGAACAAGT AATGTCATCC AACCACTGTT CGCCGTCAGG GTCTCCACAC
    CGAGTAGATG TTATAGGAAG GACTAGATCT TGGTCACCCA GTGTCCCTCC TCCACAGAGT
    CGAAATGTGG AGCAGGGACT TCGAGGGACA CGCTCTGCTA TAGGACATTA TAATACAATT
    AGCCGAATGG ATAGACACCG TGTCATGGAT GACCATTATT CTCCAGACAG AGACAGGGAT
    TGTGAAGCAG CAGATAGACA GCCATATCAC AGATCCAGAT CAACAGAACA GCGGCCTCTC
    CTAGAGCGGA CCACCACCCG CTCCAGATCC ACTGAACGTC CTGATACAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ATCGAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857431.1
    CDS373..4494
    Translation

    Target ORF information:

    RefSeq Version XM_025001663.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAATAA AACTGTGGTT TGACAAGGTT
    GGTCACCAAT TAATAGTTAC AATTTTGGGA GCAAAAGACC TCCCTTCCAG GGAAGATGGG
    AGGCCAAGGA ATCCTTATGT TAAAATTTAC TTTCTTCCAG ACAGAAGTGA CAAAAACAAG
    AGAAGAACTA AAACAGTGAA GAAAACTTTG GAACCCAAAT GGAACCAAAC ATTCATTTAT
    TCTCCAGTCC ACCGAAGAGA GTTTCGGGAA CGGATGCTGG AGATTACCCT TTGGGATCAA
    GCTCGAGTTC GAGAGGAAGA AAGTGAATTT TTAGGAGAGA TTTTAATTGA ATTAGAAACA
    GCATTATTAG ATGATGAGCC ACATTGGTAC AAACTTCAGA CTCACGATGT TTCTTCATTG
    CCACTTCCCC ATCCTTCACC ATATATGCCA CGACGACAAC TCCATGGAGA GAGCCCAACA
    CGGAGGTTGC AAAGATCAAA GAGAATAAGT GATAGTGAGG TCTCTGATTA TGACTGTGAT
    GATGGAATTG GTGTAGTATC AGATTATCGA CACAATGGTC GGGATCTTCA AAGCTCAACA
    TTGTCAGTGC CAGAACAAGT AATGTCATCC AACCACTGTT CGCCGTCAGG GTCTCCACAC
    CGAGTAGATG TTATAGGAAG GACTAGATCT TGGTCACCCA GTGTCCCTCC TCCACAGAGT
    CGAAATGTGG AGCAGGGACT TCGAGGGACA CGCTCTGCTA TAGGACATTA TAATACAATT
    AGCCGAATGG ATAGACACCG TGTCATGGAT GACCATTATT CTCCAGACAG AGACAGGGAT
    TGTGAAGCAG CAGATAGACA GCCATATCAC AGATCCAGAT CAACAGAACA GCGGCCTCTC
    CTAGAGCGGA CCACCACCCG CTCCAGATCC ACTGAACGTC CTGATACAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ATCGAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222369
    Clone ID Related Accession (Same CDS sequence) XM_025001664.1
    Accession Version XM_025001664.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA ACAATGGAGT CAAGGAGATA GAACCTTATG AAGGTGCTAT GGATATAGAG
    GAGAGAAATC GCCAAATGAA AATTAACAAA TACAAACAGG TAGCCGGATC AGATCCCAGA
    CTGGAACAAG ATTACCATGC AAAGTATCGA TCGGGATGGG ATCCTCATAG AGGGGCAGAT
    AATATTTCCA CTAAATCTTC GGACAGTGAC GTAAGTGATA TATCTGCGGT TTCCAGGACT
    AGTAGTGCTT CTCGTTTCAG CAGCACAAGC TACATGTCTG TCCAATCAGA ACGGCCAAGA
    GGAAACAAGA AAATCAGTGT CTTTACATCC AAAATGCAAA GCAGACAAAT GGGCATCTCA
    GGGAAGAACA TGACCAAGAG CACCAGCATC AGCGGAGACA TGTGCTCGCT GGAGAAGAAT
    GATGGCAGCC AGTCGGACAC CGCAGTGGGT GCCTTGGGCA CCAGCAGCAA GAAGCGACGC
    TCTAGCATTG GGGCCAAAAT GGTAGCTATC GTTGGTCTTT CCCGGAAGAG CCGCAGTGCT
    TCTCAGCTCA GCCAAACGGA AACCGGAGGT AAAAAGCTAC GGAGCACTGT CCAGAGAAGT
    ACAGAAACAG GCTTGGCTGT GGAAATGAGG AACTGGATGA CTCGACAAGC GAGCCGAGAA
    TCTACCGACG GCAGCATGAA CAGCTACAGC TCAGAAGGAA ATCTGATCTT CCCTGGTGTC
    CGCTTGGCCT CGGATAGCCA ATTCAGTGAT TTTCTGGATG GCCTTGGCCC TGCCCAGCTA
    GTGGGGCGCC AAACTCTGGC CACACCCGCC ATGGGTGACA TTCAGGTAGG AATGATGGAC
    AAAAAGGGAC AGCTGGAGGT GGAAATAATT CGGGCCCGTG GCCTTGTTGT AAAACCAGGT
    TCCAAGACAC TGCCAGCACC ATACGTAAAG GTGTATCTAT TAGATAATGG AGTCTGCATA
    GCCAAAAAGA AAACAAAAGT GGCAAGAAAA ACGCTGGAAC CCCTTTACCA GCAACTATTA
    TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT TTACAGATCA TTGTCTGGGG AGACTATGGC
    CGCATGGATC ACAAATCCTT TATGGGAGTG GCCCAGATAC TTTTAGATGA ACTGGAGCTA
    TCCAACATGG TGATCGGCTG GTTCAAACTC TTCCCCCCTT CCTCCCTAGT AGATCCAACC
    TTGGCCCCTC TGACAAGAAG AGCTTCCCAA TCATCTCTGG AAAGTTCAAC TGGACCTTCT
    TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857432.1
    CDS1..3975
    Translation

    Target ORF information:

    RefSeq Version XM_025001664.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001664.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA ACAATGGAGT CAAGGAGATA GAACCTTATG AAGGTGCTAT GGATATAGAG
    GAGAGAAATC GCCAAATGAA AATTAACAAA TACAAACAGG TAGCCGGATC AGATCCCAGA
    CTGGAACAAG ATTACCATGC AAAGTATCGA TCGGGATGGG ATCCTCATAG AGGGGCAGAT
    AATATTTCCA CTAAATCTTC GGACAGTGAC GTAAGTGATA TATCTGCGGT TTCCAGGACT
    AGTAGTGCTT CTCGTTTCAG CAGCACAAGC TACATGTCTG TCCAATCAGA ACGGCCAAGA
    GGAAACAAGA AAATCAGTGT CTTTACATCC AAAATGCAAA GCAGACAAAT GGGCATCTCA
    GGGAAGAACA TGACCAAGAG CACCAGCATC AGCGGAGACA TGTGCTCGCT GGAGAAGAAT
    GATGGCAGCC AGTCGGACAC CGCAGTGGGT GCCTTGGGCA CCAGCAGCAA GAAGCGACGC
    TCTAGCATTG GGGCCAAAAT GGTAGCTATC GTTGGTCTTT CCCGGAAGAG CCGCAGTGCT
    TCTCAGCTCA GCCAAACGGA AACCGGAGGT AAAAAGCTAC GGAGCACTGT CCAGAGAAGT
    ACAGAAACAG GCTTGGCTGT GGAAATGAGG AACTGGATGA CTCGACAAGC GAGCCGAGAA
    TCTACCGACG GCAGCATGAA CAGCTACAGC TCAGAAGGAA ATCTGATCTT CCCTGGTGTC
    CGCTTGGCCT CGGATAGCCA ATTCAGTGAT TTTCTGGATG GCCTTGGCCC TGCCCAGCTA
    GTGGGGCGCC AAACTCTGGC CACACCCGCC ATGGGTGACA TTCAGGTAGG AATGATGGAC
    AAAAAGGGAC AGCTGGAGGT GGAAATAATT CGGGCCCGTG GCCTTGTTGT AAAACCAGGT
    TCCAAGACAC TGCCAGCACC ATACGTAAAG GTGTATCTAT TAGATAATGG AGTCTGCATA
    GCCAAAAAGA AAACAAAAGT GGCAAGAAAA ACGCTGGAAC CCCTTTACCA GCAACTATTA
    TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT TTACAGATCA TTGTCTGGGG AGACTATGGC
    CGCATGGATC ACAAATCCTT TATGGGAGTG GCCCAGATAC TTTTAGATGA ACTGGAGCTA
    TCCAACATGG TGATCGGCTG GTTCAAACTC TTCCCCCCTT CCTCCCTAGT AGATCCAACC
    TTGGCCCCTC TGACAAGAAG AGCTTCCCAA TCATCTCTGG AAAGTTCAAC TGGACCTTCT
    TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222370
    Clone ID Related Accession (Same CDS sequence) XM_025001665.1
    Accession Version XM_025001665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAATAA AACTGTGGTT TGACAAGGTT
    GGTCACCAAT TAATAGTTAC AATTTTGGGA GCAAAAGACC TCCCTTCCAG GGAAGATGGG
    AGGCCAAGGA ATCCTTATGT TAAAATTTAC TTTCTTCCAG ACAGAAGTGA CAAAAACAAG
    AGAAGAACTA AAACAGTGAA GAAAACTTTG GAACCCAAAT GGAACCAAAC ATTCATTTAT
    TCTCCAGTCC ACCGAAGAGA GTTTCGGGAA CGGATGCTGG AGATTACCCT TTGGGATCAA
    GCTCGAGTTC GAGAGGAAGA AAGTGAATTT TTAGGAGAGA TTTTAATTGA ATTAGAAACA
    GCATTATTAG ATGATGAGCC ACATTGGTAC AAACTTCAGA CTCACGATGT TTCTTCATTG
    CCACTTCCCC ATCCTTCACC ATATATGCCA CGACGACAAC TCCATGGAGA GAGCCCAACA
    CGGAGGTTGC AAAGATCAAA GAGAATAAGT GATAGTGAGG TCTCTGATTA TGACTGTGAT
    GATGGAATTG GTGTAGTATC AGATTATCGA CACAATGGTC GGGATCTTCA AAGCTCAACA
    TTGTCAGTGC CAGAACAAGT AATGTCATCC AACCACTGTT CGCCGTCAGG GTCTCCACAC
    CGAGTAGATG TTATAGGAAG GACTAGATCT TGGTCACCCA GTGTCCCTCC TCCACAGAGT
    CGAAATGTGG AGCAGGGACT TCGAGGGACA CGCTCTGCTA TAGGACATTA TAATACAATT
    AGCCGAATGG ATAGACACCG TGTCATGGAT GACCATTATT CTCCAGACAG AGACAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857433.1
    CDS373..4314
    Translation

    Target ORF information:

    RefSeq Version XM_025001665.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAATAA AACTGTGGTT TGACAAGGTT
    GGTCACCAAT TAATAGTTAC AATTTTGGGA GCAAAAGACC TCCCTTCCAG GGAAGATGGG
    AGGCCAAGGA ATCCTTATGT TAAAATTTAC TTTCTTCCAG ACAGAAGTGA CAAAAACAAG
    AGAAGAACTA AAACAGTGAA GAAAACTTTG GAACCCAAAT GGAACCAAAC ATTCATTTAT
    TCTCCAGTCC ACCGAAGAGA GTTTCGGGAA CGGATGCTGG AGATTACCCT TTGGGATCAA
    GCTCGAGTTC GAGAGGAAGA AAGTGAATTT TTAGGAGAGA TTTTAATTGA ATTAGAAACA
    GCATTATTAG ATGATGAGCC ACATTGGTAC AAACTTCAGA CTCACGATGT TTCTTCATTG
    CCACTTCCCC ATCCTTCACC ATATATGCCA CGACGACAAC TCCATGGAGA GAGCCCAACA
    CGGAGGTTGC AAAGATCAAA GAGAATAAGT GATAGTGAGG TCTCTGATTA TGACTGTGAT
    GATGGAATTG GTGTAGTATC AGATTATCGA CACAATGGTC GGGATCTTCA AAGCTCAACA
    TTGTCAGTGC CAGAACAAGT AATGTCATCC AACCACTGTT CGCCGTCAGG GTCTCCACAC
    CGAGTAGATG TTATAGGAAG GACTAGATCT TGGTCACCCA GTGTCCCTCC TCCACAGAGT
    CGAAATGTGG AGCAGGGACT TCGAGGGACA CGCTCTGCTA TAGGACATTA TAATACAATT
    AGCCGAATGG ATAGACACCG TGTCATGGAT GACCATTATT CTCCAGACAG AGACAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAAAAC CGGAGGTAAA
    AAGCTACGGA GCACTGTCCA GAGAAGTACA GAAACAGGCT TGGCTGTGGA AATGAGGAAC
    TGGATGACTC GACAAGCGAG CCGAGAATCT ACCGACGGCA GCATGAACAG CTACAGCTCA
    GAAGGAAATC TGATCTTCCC TGGTGTCCGC TTGGCCTCGG ATAGCCAATT CAGTGATTTT
    CTGGATGGCC TTGGCCCTGC CCAGCTAGTG GGGCGCCAAA CTCTGGCCAC ACCCGCCATG
    GGTGACATTC AGGTAGGAAT GATGGACAAA AAGGGACAGC TGGAGGTGGA AATAATTCGG
    GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC AAGACACTGC CAGCACCATA CGTAAAGGTG
    TATCTATTAG ATAATGGAGT CTGCATAGCC AAAAAGAAAA CAAAAGTGGC AAGAAAAACG
    CTGGAACCCC TTTACCAGCA ACTATTATCT TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA
    CAGATCATTG TCTGGGGAGA CTATGGCCGC ATGGATCACA AATCCTTTAT GGGAGTGGCC
    CAGATACTTT TAGATGAACT GGAGCTATCC AACATGGTGA TCGGCTGGTT CAAACTCTTC
    CCCCCTTCCT CCCTAGTAGA TCCAACCTTG GCCCCTCTGA CAAGAAGAGC TTCCCAATCA
    TCTCTGGAAA GTTCAACTGG ACCTTCTTAC TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222371
    Clone ID Related Accession (Same CDS sequence) XM_025001666.1
    Accession Version XM_025001666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GTCATTTTCT TACTCTACCT
    CGCTCCAGAT ACAGTCAGAG CGTTGAACAT CATCACAGGG ATGGCAGGGA TTGTGAAGCA
    GCAGATAGAC AGCCATATCA CAGATCCAGA TCAACAGAAC AGCGGCCTCT CCTAGAGCGG
    ACCACCACCC GCTCCAGATC CACTGAACGT CCTGATACAA ACCTCATGAG GTCGATGCCT
    TCATTAATGA CTGGAAGATC TGCCCCTCCT TCACCTGCCT TATCGAGGTC TCATCCTCGT
    ACTGGGTCTG TCCAAACAAG CCCATCAAGT ACTCCAGTGG CAGGACGAAG GGGCCGACAA
    CTCCCACAGC TTCCACCAAA GGGAACATTG GAGAGAAAAA CCGGAGGTAA AAAGCTACGG
    AGCACTGTCC AGAGAAGTAC AGAAACAGGC TTGGCTGTGG AAATGAGGAA CTGGATGACT
    CGACAAGCGA GCCGAGAATC TACCGACGGC AGCATGAACA GCTACAGCTC AGAAGGAAAT
    CTGATCTTCC CTGGTGTCCG CTTGGCCTCG GATAGCCAAT TCAGTGATTT TCTGGATGGC
    CTTGGCCCTG CCCAGCTAGT GGGGCGCCAA ACTCTGGCCA CACCCGCCAT GGGTGACATT
    CAGGTAGGAA TGATGGACAA AAAGGGACAG CTGGAGGTGG AAATAATTCG GGCCCGTGGC
    CTTGTTGTAA AACCAGGTTC CAAGACACTG CCAGCACCAT ACGTAAAGGT GTATCTATTA
    GATAATGGAG TCTGCATAGC CAAAAAGAAA ACAAAAGTGG CAAGAAAAAC GCTGGAACCC
    CTTTACCAGC AACTATTATC TTTTGAAGAA AGTCCCCAAG GAAAGGTTTT ACAGATCATT
    GTCTGGGGAG ACTATGGCCG CATGGATCAC AAATCCTTTA TGGGAGTGGC CCAGATACTT
    TTAGATGAAC TGGAGCTATC CAACATGGTG ATCGGCTGGT TCAAACTCTT CCCCCCTTCC
    TCCCTAGTAG ATCCAACCTT GGCCCCTCTG ACAAGAAGAG CTTCCCAATC ATCTCTGGAA
    AGTTCAACTG GACCTTCTTA CTCTCGTTCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857434.1
    CDS2343..5975
    Translation

    Target ORF information:

    RefSeq Version XM_025001666.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GTCATTTTCT TACTCTACCT
    CGCTCCAGAT ACAGTCAGAG CGTTGAACAT CATCACAGGG ATGGCAGGGA TTGTGAAGCA
    GCAGATAGAC AGCCATATCA CAGATCCAGA TCAACAGAAC AGCGGCCTCT CCTAGAGCGG
    ACCACCACCC GCTCCAGATC CACTGAACGT CCTGATACAA ACCTCATGAG GTCGATGCCT
    TCATTAATGA CTGGAAGATC TGCCCCTCCT TCACCTGCCT TATCGAGGTC TCATCCTCGT
    ACTGGGTCTG TCCAAACAAG CCCATCAAGT ACTCCAGTGG CAGGACGAAG GGGCCGACAA
    CTCCCACAGC TTCCACCAAA GGGAACATTG GAGAGAAAAA CCGGAGGTAA AAAGCTACGG
    AGCACTGTCC AGAGAAGTAC AGAAACAGGC TTGGCTGTGG AAATGAGGAA CTGGATGACT
    CGACAAGCGA GCCGAGAATC TACCGACGGC AGCATGAACA GCTACAGCTC AGAAGGAAAT
    CTGATCTTCC CTGGTGTCCG CTTGGCCTCG GATAGCCAAT TCAGTGATTT TCTGGATGGC
    CTTGGCCCTG CCCAGCTAGT GGGGCGCCAA ACTCTGGCCA CACCCGCCAT GGGTGACATT
    CAGGTAGGAA TGATGGACAA AAAGGGACAG CTGGAGGTGG AAATAATTCG GGCCCGTGGC
    CTTGTTGTAA AACCAGGTTC CAAGACACTG CCAGCACCAT ACGTAAAGGT GTATCTATTA
    GATAATGGAG TCTGCATAGC CAAAAAGAAA ACAAAAGTGG CAAGAAAAAC GCTGGAACCC
    CTTTACCAGC AACTATTATC TTTTGAAGAA AGTCCCCAAG GAAAGGTTTT ACAGATCATT
    GTCTGGGGAG ACTATGGCCG CATGGATCAC AAATCCTTTA TGGGAGTGGC CCAGATACTT
    TTAGATGAAC TGGAGCTATC CAACATGGTG ATCGGCTGGT TCAAACTCTT CCCCCCTTCC
    TCCCTAGTAG ATCCAACCTT GGCCCCTCTG ACAAGAAGAG CTTCCCAATC ATCTCTGGAA
    AGTTCAACTG GACCTTCTTA CTCTCGTTCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222372
    Clone ID Related Accession (Same CDS sequence) XM_025001667.1
    Accession Version XM_025001667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA GTCAGAGCGT TGAACATCAT
    CACAGGGATG GCAGGGATTG TGAAGCAGCA GATAGACAGC CATATCACAG ATCCAGATCA
    ACAGAACAGC GGCCTCTCCT AGAGCGGACC ACCACCCGCT CCAGATCCAC TGAACGTCCT
    GATACAAACC TCATGAGGTC GATGCCTTCA TTAATGACTG GAAGATCTGC CCCTCCTTCA
    CCTGCCTTAT CGAGGTCTCA TCCTCGTACT GGGTCTGTCC AAACAAGCCC ATCAAGTACT
    CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC CACCAAAGGG AACATTGGAG
    AGAAAAACCG GAGGTAAAAA GCTACGGAGC ACTGTCCAGA GAAGTACAGA AACAGGCTTG
    GCTGTGGAAA TGAGGAACTG GATGACTCGA CAAGCGAGCC GAGAATCTAC CGACGGCAGC
    ATGAACAGCT ACAGCTCAGA AGGAAATCTG ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT
    AGCCAATTCA GTGATTTTCT GGATGGCCTT GGCCCTGCCC AGCTAGTGGG GCGCCAAACT
    CTGGCCACAC CCGCCATGGG TGACATTCAG GTAGGAATGA TGGACAAAAA GGGACAGCTG
    GAGGTGGAAA TAATTCGGGC CCGTGGCCTT GTTGTAAAAC CAGGTTCCAA GACACTGCCA
    GCACCATACG TAAAGGTGTA TCTATTAGAT AATGGAGTCT GCATAGCCAA AAAGAAAACA
    AAAGTGGCAA GAAAAACGCT GGAACCCCTT TACCAGCAAC TATTATCTTT TGAAGAAAGT
    CCCCAAGGAA AGGTTTTACA GATCATTGTC TGGGGAGACT ATGGCCGCAT GGATCACAAA
    TCCTTTATGG GAGTGGCCCA GATACTTTTA GATGAACTGG AGCTATCCAA CATGGTGATC
    GGCTGGTTCA AACTCTTCCC CCCTTCCTCC CTAGTAGATC CAACCTTGGC CCCTCTGACA
    AGAAGAGCTT CCCAATCATC TCTGGAAAGT TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857435.1
    CDS2343..5882
    Translation

    Target ORF information:

    RefSeq Version XM_025001667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA GTCAGAGCGT TGAACATCAT
    CACAGGGATG GCAGGGATTG TGAAGCAGCA GATAGACAGC CATATCACAG ATCCAGATCA
    ACAGAACAGC GGCCTCTCCT AGAGCGGACC ACCACCCGCT CCAGATCCAC TGAACGTCCT
    GATACAAACC TCATGAGGTC GATGCCTTCA TTAATGACTG GAAGATCTGC CCCTCCTTCA
    CCTGCCTTAT CGAGGTCTCA TCCTCGTACT GGGTCTGTCC AAACAAGCCC ATCAAGTACT
    CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC CACCAAAGGG AACATTGGAG
    AGAAAAACCG GAGGTAAAAA GCTACGGAGC ACTGTCCAGA GAAGTACAGA AACAGGCTTG
    GCTGTGGAAA TGAGGAACTG GATGACTCGA CAAGCGAGCC GAGAATCTAC CGACGGCAGC
    ATGAACAGCT ACAGCTCAGA AGGAAATCTG ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT
    AGCCAATTCA GTGATTTTCT GGATGGCCTT GGCCCTGCCC AGCTAGTGGG GCGCCAAACT
    CTGGCCACAC CCGCCATGGG TGACATTCAG GTAGGAATGA TGGACAAAAA GGGACAGCTG
    GAGGTGGAAA TAATTCGGGC CCGTGGCCTT GTTGTAAAAC CAGGTTCCAA GACACTGCCA
    GCACCATACG TAAAGGTGTA TCTATTAGAT AATGGAGTCT GCATAGCCAA AAAGAAAACA
    AAAGTGGCAA GAAAAACGCT GGAACCCCTT TACCAGCAAC TATTATCTTT TGAAGAAAGT
    CCCCAAGGAA AGGTTTTACA GATCATTGTC TGGGGAGACT ATGGCCGCAT GGATCACAAA
    TCCTTTATGG GAGTGGCCCA GATACTTTTA GATGAACTGG AGCTATCCAA CATGGTGATC
    GGCTGGTTCA AACTCTTCCC CCCTTCCTCC CTAGTAGATC CAACCTTGGC CCCTCTGACA
    AGAAGAGCTT CCCAATCATC TCTGGAAAGT TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222373
    Clone ID Related Accession (Same CDS sequence) XM_025001668.1
    Accession Version XM_025001668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGGG ATTGTGAAGC AGCAGATAGA CAGCCATATC ACAGATCCAG ATCAACAGAA
    CAGCGGCCTC TCCTAGAGCG GACCACCACC CGCTCCAGAT CCACTGAACG TCCTGATACA
    AACCTCATGA GGTCGATGCC TTCATTAATG ACTGGAAGAT CTGCCCCTCC TTCACCTGCC
    TTATCGAGGT CTCATCCTCG TACTGGGTCT GTCCAAACAA GCCCATCAAG TACTCCAGTG
    GCAGGACGAA GGGGCCGACA ACTCCCACAG CTTCCACCAA AGGGAACATT GGAGAGAAAA
    ACCGGAGGTA AAAAGCTACG GAGCACTGTC CAGAGAAGTA CAGAAACAGG CTTGGCTGTG
    GAAATGAGGA ACTGGATGAC TCGACAAGCG AGCCGAGAAT CTACCGACGG CAGCATGAAC
    AGCTACAGCT CAGAAGGAAA TCTGATCTTC CCTGGTGTCC GCTTGGCCTC GGATAGCCAA
    TTCAGTGATT TTCTGGATGG CCTTGGCCCT GCCCAGCTAG TGGGGCGCCA AACTCTGGCC
    ACACCCGCCA TGGGTGACAT TCAGGTAGGA ATGATGGACA AAAAGGGACA GCTGGAGGTG
    GAAATAATTC GGGCCCGTGG CCTTGTTGTA AAACCAGGTT CCAAGACACT GCCAGCACCA
    TACGTAAAGG TGTATCTATT AGATAATGGA GTCTGCATAG CCAAAAAGAA AACAAAAGTG
    GCAAGAAAAA CGCTGGAACC CCTTTACCAG CAACTATTAT CTTTTGAAGA AAGTCCCCAA
    GGAAAGGTTT TACAGATCAT TGTCTGGGGA GACTATGGCC GCATGGATCA CAAATCCTTT
    ATGGGAGTGG CCCAGATACT TTTAGATGAA CTGGAGCTAT CCAACATGGT GATCGGCTGG
    TTCAAACTCT TCCCCCCTTC CTCCCTAGTA GATCCAACCT TGGCCCCTCT GACAAGAAGA
    GCTTCCCAAT CATCTCTGGA AAGTTCAACT GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857436.1
    CDS2342..5815
    Translation

    Target ORF information:

    RefSeq Version XM_025001668.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGGG ATTGTGAAGC AGCAGATAGA CAGCCATATC ACAGATCCAG ATCAACAGAA
    CAGCGGCCTC TCCTAGAGCG GACCACCACC CGCTCCAGAT CCACTGAACG TCCTGATACA
    AACCTCATGA GGTCGATGCC TTCATTAATG ACTGGAAGAT CTGCCCCTCC TTCACCTGCC
    TTATCGAGGT CTCATCCTCG TACTGGGTCT GTCCAAACAA GCCCATCAAG TACTCCAGTG
    GCAGGACGAA GGGGCCGACA ACTCCCACAG CTTCCACCAA AGGGAACATT GGAGAGAAAA
    ACCGGAGGTA AAAAGCTACG GAGCACTGTC CAGAGAAGTA CAGAAACAGG CTTGGCTGTG
    GAAATGAGGA ACTGGATGAC TCGACAAGCG AGCCGAGAAT CTACCGACGG CAGCATGAAC
    AGCTACAGCT CAGAAGGAAA TCTGATCTTC CCTGGTGTCC GCTTGGCCTC GGATAGCCAA
    TTCAGTGATT TTCTGGATGG CCTTGGCCCT GCCCAGCTAG TGGGGCGCCA AACTCTGGCC
    ACACCCGCCA TGGGTGACAT TCAGGTAGGA ATGATGGACA AAAAGGGACA GCTGGAGGTG
    GAAATAATTC GGGCCCGTGG CCTTGTTGTA AAACCAGGTT CCAAGACACT GCCAGCACCA
    TACGTAAAGG TGTATCTATT AGATAATGGA GTCTGCATAG CCAAAAAGAA AACAAAAGTG
    GCAAGAAAAA CGCTGGAACC CCTTTACCAG CAACTATTAT CTTTTGAAGA AAGTCCCCAA
    GGAAAGGTTT TACAGATCAT TGTCTGGGGA GACTATGGCC GCATGGATCA CAAATCCTTT
    ATGGGAGTGG CCCAGATACT TTTAGATGAA CTGGAGCTAT CCAACATGGT GATCGGCTGG
    TTCAAACTCT TCCCCCCTTC CTCCCTAGTA GATCCAACCT TGGCCCCTCT GACAAGAAGA
    GCTTCCCAAT CATCTCTGGA AAGTTCAACT GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222374
    Clone ID Related Accession (Same CDS sequence) XM_025001669.1
    Accession Version XM_025001669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAACCGGAGG TAAAAAGCTA CGGAGCACTG TCCAGAGAAG TACAGAAACA
    GGCTTGGCTG TGGAAATGAG GAACTGGATG ACTCGACAAG CGAGCCGAGA ATCTACCGAC
    GGCAGCATGA ACAGCTACAG CTCAGAAGGA AATCTGATCT TCCCTGGTGT CCGCTTGGCC
    TCGGATAGCC AATTCAGTGA TTTTCTGGAT GGCCTTGGCC CTGCCCAGCT AGTGGGGCGC
    CAAACTCTGG CCACACCCGC CATGGGTGAC ATTCAGGTAG GAATGATGGA CAAAAAGGGA
    CAGCTGGAGG TGGAAATAAT TCGGGCCCGT GGCCTTGTTG TAAAACCAGG TTCCAAGACA
    CTGCCAGCAC CATACGTAAA GGTGTATCTA TTAGATAATG GAGTCTGCAT AGCCAAAAAG
    AAAACAAAAG TGGCAAGAAA AACGCTGGAA CCCCTTTACC AGCAACTATT ATCTTTTGAA
    GAAAGTCCCC AAGGAAAGGT TTTACAGATC ATTGTCTGGG GAGACTATGG CCGCATGGAT
    CACAAATCCT TTATGGGAGT GGCCCAGATA CTTTTAGATG AACTGGAGCT ATCCAACATG
    GTGATCGGCT GGTTCAAACT CTTCCCCCCT TCCTCCCTAG TAGATCCAAC CTTGGCCCCT
    CTGACAAGAA GAGCTTCCCA ATCATCTCTG GAAAGTTCAA CTGGACCTTC TTACTCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857437.1
    CDS2342..5767
    Translation

    Target ORF information:

    RefSeq Version XM_025001669.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAACCGGAGG TAAAAAGCTA CGGAGCACTG TCCAGAGAAG TACAGAAACA
    GGCTTGGCTG TGGAAATGAG GAACTGGATG ACTCGACAAG CGAGCCGAGA ATCTACCGAC
    GGCAGCATGA ACAGCTACAG CTCAGAAGGA AATCTGATCT TCCCTGGTGT CCGCTTGGCC
    TCGGATAGCC AATTCAGTGA TTTTCTGGAT GGCCTTGGCC CTGCCCAGCT AGTGGGGCGC
    CAAACTCTGG CCACACCCGC CATGGGTGAC ATTCAGGTAG GAATGATGGA CAAAAAGGGA
    CAGCTGGAGG TGGAAATAAT TCGGGCCCGT GGCCTTGTTG TAAAACCAGG TTCCAAGACA
    CTGCCAGCAC CATACGTAAA GGTGTATCTA TTAGATAATG GAGTCTGCAT AGCCAAAAAG
    AAAACAAAAG TGGCAAGAAA AACGCTGGAA CCCCTTTACC AGCAACTATT ATCTTTTGAA
    GAAAGTCCCC AAGGAAAGGT TTTACAGATC ATTGTCTGGG GAGACTATGG CCGCATGGAT
    CACAAATCCT TTATGGGAGT GGCCCAGATA CTTTTAGATG AACTGGAGCT ATCCAACATG
    GTGATCGGCT GGTTCAAACT CTTCCCCCCT TCCTCCCTAG TAGATCCAAC CTTGGCCCCT
    CTGACAAGAA GAGCTTCCCA ATCATCTCTG GAAAGTTCAA CTGGACCTTC TTACTCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222375
    Clone ID Related Accession (Same CDS sequence) XM_025001670.1
    Accession Version XM_025001670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAACCGGAGG TAAAAAGCTA CGGAGCACTG TCCAGAGAAG TACAGAAACA
    GGCTTGGCTG TGGAAATGAG GAACTGGATG ACTCGACAAG CGAGCCGAGA ATCTACCGAC
    GGCAGCATGA ACAGCTACAG CTCAGAAGGA AATCTGATCT TCCCTGGTGT CCGCTTGGCC
    TCGGATAGCC AATTCAGTGA TTTTCTGGAT GGCCTTGGCC CTGCCCAGCT AGTGGGGCGC
    CAAACTCTGG CCACACCCGC CATGGGTGAC ATTCAGGTAG GAATGATGGA CAAAAAGGGA
    CAGCTGGAGG TGGAAATAAT TCGGGCCCGT GGCCTTGTTG TAAAACCAGG TTCCAAGACA
    CTGCCAGCAC CATACGTAAA GGTGTATCTA TTAGATAATG GAGTCTGCAT AGCCAAAAAG
    AAAACAAAAG TGGCAAGAAA AACGCTGGAA CCCCTTTACC AGCAACTATT ATCTTTTGAA
    GAAAGTCCCC AAGGAAAGGT TTTACAGATC ATTGTCTGGG GAGACTATGG CCGCATGGAT
    CACAAATCCT TTATGGGAGT GGCCCAGATA CTTTTAGATG AACTGGAGCT ATCCAACATG
    GTGATCGGCT GGTTCAAACT CTTCCCCCCT TCCTCCCTAG TAGATCCAAC CTTGGCCCCT
    CTGACAAGAA GAGCTTCCCA ATCATCTCTG GAAAGTTCAA CTGGACCTTC TTACTCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857438.1
    CDS2341..5586
    Translation

    Target ORF information:

    RefSeq Version XM_025001670.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAACCGGAGG TAAAAAGCTA CGGAGCACTG TCCAGAGAAG TACAGAAACA
    GGCTTGGCTG TGGAAATGAG GAACTGGATG ACTCGACAAG CGAGCCGAGA ATCTACCGAC
    GGCAGCATGA ACAGCTACAG CTCAGAAGGA AATCTGATCT TCCCTGGTGT CCGCTTGGCC
    TCGGATAGCC AATTCAGTGA TTTTCTGGAT GGCCTTGGCC CTGCCCAGCT AGTGGGGCGC
    CAAACTCTGG CCACACCCGC CATGGGTGAC ATTCAGGTAG GAATGATGGA CAAAAAGGGA
    CAGCTGGAGG TGGAAATAAT TCGGGCCCGT GGCCTTGTTG TAAAACCAGG TTCCAAGACA
    CTGCCAGCAC CATACGTAAA GGTGTATCTA TTAGATAATG GAGTCTGCAT AGCCAAAAAG
    AAAACAAAAG TGGCAAGAAA AACGCTGGAA CCCCTTTACC AGCAACTATT ATCTTTTGAA
    GAAAGTCCCC AAGGAAAGGT TTTACAGATC ATTGTCTGGG GAGACTATGG CCGCATGGAT
    CACAAATCCT TTATGGGAGT GGCCCAGATA CTTTTAGATG AACTGGAGCT ATCCAACATG
    GTGATCGGCT GGTTCAAACT CTTCCCCCCT TCCTCCCTAG TAGATCCAAC CTTGGCCCCT
    CTGACAAGAA GAGCTTCCCA ATCATCTCTG GAAAGTTCAA CTGGACCTTC TTACTCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222377
    Clone ID Related Accession (Same CDS sequence) XM_025001672.1
    Accession Version XM_025001672.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA GTCAGAGCGT TGAACATCAT
    CACAGGGATG GCAGGGATTG TGAAGCAGCA GATAGACAGC CATATCACAG ATCCAGATCA
    ACAGAACAGC GGCCTCTCCT AGAGCGGACC ACCACCCGCT CCAGATCCAC TGAACGTCCT
    GATACAAACC TCATGAGGTC GATGCCTTCA TTAATGACTG GAAGATCTGC CCCTCCTTCA
    CCTGCCTTAT CGAGGTCTCA TCCTCGTACT GGGTCTGTCC AAACAAGCCC ATCAAGTACT
    CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC CACCAAAGGG AACATTGGAG
    AGAATGATCA CAGAAGACGT GGACGCCACA AGGAGAAGAC ATGCAGGTGC TATGGATATA
    GAGGAGAGAA ATCGCCAAAT GAAAATTAAC AAATACAAAC AGGTAGCCGG ATCAGATCCC
    AGACTGGAAC AAGATTACCA TGCAAAGTAT CGATCGGGAT GGGATCCTCA TAGAGGGGCA
    GATAATATTT CCACTAAATC TTCGGACAGT GACGTAAGTG ATATATCTGC GGTTTCCAGG
    ACTAGTAGTG CTTCTCGTTT CAGCAGCACA AGCTACATGT CTGTCCAATC AGAACGGCCA
    AGAGGAAACA AGAAAATCAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857440.1
    CDS2344..6441
    Translation

    Target ORF information:

    RefSeq Version XM_025001672.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG
    TTTGACAAGG TTGGTCACCA ATTAATAGTT ACAATTTTGG GAGCAAAAGA CCTCCCTTCC
    AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT
    GACAAAAACA AGAGAAGAAC TAAAACAGTG AAGAAAACTT TGGAACCCAA ATGGAACCAA
    ACATTCATTT ATTCTCCAGT CCACCGAAGA GAGTTTCGGG AACGGATGCT GGAGATTACC
    CTTTGGGATC AAGCTCGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTTTAATT
    GAATTAGAAA CAGCATTATT AGATGATGAG CCACATTGGT ACAAACTTCA GACTCACGAT
    GTTTCTTCAT TGCCACTTCC CCATCCTTCA CCATATATGC CACGACGACA ACTCCATGGA
    GAGAGCCCAA CACGGAGGTT GCAAAGATCA AAGAGAATAA GTGATAGTGA GGTCTCTGAT
    TATGACTGTG ATGATGGAAT TGGTGTAGTA TCAGATTATC GACACAATGG TCGGGATCTT
    CAAAGCTCAA CATTGTCAGT GCCAGAACAA GTAATGTCAT CCAACCACTG TTCGCCGTCA
    GGGTCTCCAC ACCGAGTAGA TGTTATAGGA AGGACTAGAT CTTGGTCACC CAGTGTCCCT
    CCTCCACAGA GTCGAAATGT GGAGCAGGGA CTTCGAGGGA CACGCTCTGC TATAGGACAT
    TATAATACAA TTAGCCGAAT GGATAGACAC CGTGTCATGG ATGACCATTA TTCTCCAGAC
    AGAGACAGTC ATTTTCTTAC TCTACCTCGC TCCAGATACA GTCAGAGCGT TGAACATCAT
    CACAGGGATG GCAGGGATTG TGAAGCAGCA GATAGACAGC CATATCACAG ATCCAGATCA
    ACAGAACAGC GGCCTCTCCT AGAGCGGACC ACCACCCGCT CCAGATCCAC TGAACGTCCT
    GATACAAACC TCATGAGGTC GATGCCTTCA TTAATGACTG GAAGATCTGC CCCTCCTTCA
    CCTGCCTTAT CGAGGTCTCA TCCTCGTACT GGGTCTGTCC AAACAAGCCC ATCAAGTACT
    CCAGTGGCAG GACGAAGGGG CCGACAACTC CCACAGCTTC CACCAAAGGG AACATTGGAG
    AGAATGATCA CAGAAGACGT GGACGCCACA AGGAGAAGAC ATGCAGGTGC TATGGATATA
    GAGGAGAGAA ATCGCCAAAT GAAAATTAAC AAATACAAAC AGGTAGCCGG ATCAGATCCC
    AGACTGGAAC AAGATTACCA TGCAAAGTAT CGATCGGGAT GGGATCCTCA TAGAGGGGCA
    GATAATATTT CCACTAAATC TTCGGACAGT GACGTAAGTG ATATATCTGC GGTTTCCAGG
    ACTAGTAGTG CTTCTCGTTT CAGCAGCACA AGCTACATGT CTGTCCAATC AGAACGGCCA
    AGAGGAAACA AGAAAATCAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222378
    Clone ID Related Accession (Same CDS sequence) XM_025001673.1
    Accession Version XM_025001673.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAACAA TGGAGTCAAG GAGATAGAAC CTTATGAAGA CGTGGACGCC
    ACAAGGAGAA GACATGCAGG TGCTATGGAT ATAGAGGAGA GAAATCGCCA AATGAAAATT
    AACAAATACA AACAGGTAGC CGGATCAGAT CCCAGACTGG AACAAGATTA CCATGCAAAG
    TATCGATCGG GATGGGATCC TCATAGAGGG GCAGATAATA TTTCCACTAA ATCTTCGGAC
    AGTGACGTAA GTGATATATC TGCGGTTTCC AGGACTAGTA GTGCTTCTCG TTTCAGCAGC
    ACAAGCTACA TGTCTGTCCA ATCAGAACGG CCAAGAGGAA ACAAGAAAAT CAGTGTCTTT
    ACATCCAAAA TGCAAAGCAG ACAAATGGGC ATCTCAGGGA AGAACATGAC CAAGAGCACC
    AGCATCAGCG GAGACATGTG CTCGCTGGAG AAGAATGATG GCAGCCAGTC GGACACCGCA
    GTGGGTGCCT TGGGCACCAG CAGCAAGAAG CGACGCTCTA GCATTGGGGC CAAAATGGTA
    GCTATCGTTG GTCTTTCCCG GAAGAGCCGC AGTGCTTCTC AGCTCAGCCA AACGGAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857441.1
    CDS2344..6414
    Translation

    Target ORF information:

    RefSeq Version XM_025001673.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAACAA TGGAGTCAAG GAGATAGAAC CTTATGAAGA CGTGGACGCC
    ACAAGGAGAA GACATGCAGG TGCTATGGAT ATAGAGGAGA GAAATCGCCA AATGAAAATT
    AACAAATACA AACAGGTAGC CGGATCAGAT CCCAGACTGG AACAAGATTA CCATGCAAAG
    TATCGATCGG GATGGGATCC TCATAGAGGG GCAGATAATA TTTCCACTAA ATCTTCGGAC
    AGTGACGTAA GTGATATATC TGCGGTTTCC AGGACTAGTA GTGCTTCTCG TTTCAGCAGC
    ACAAGCTACA TGTCTGTCCA ATCAGAACGG CCAAGAGGAA ACAAGAAAAT CAGTGTCTTT
    ACATCCAAAA TGCAAAGCAG ACAAATGGGC ATCTCAGGGA AGAACATGAC CAAGAGCACC
    AGCATCAGCG GAGACATGTG CTCGCTGGAG AAGAATGATG GCAGCCAGTC GGACACCGCA
    GTGGGTGCCT TGGGCACCAG CAGCAAGAAG CGACGCTCTA GCATTGGGGC CAAAATGGTA
    GCTATCGTTG GTCTTTCCCG GAAGAGCCGC AGTGCTTCTC AGCTCAGCCA AACGGAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222379
    Clone ID Related Accession (Same CDS sequence) XM_025001674.1
    Accession Version XM_025001674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAGACGTGGA CGCCACAAGG AGAAGACATG CAGGTGCTAT GGATATAGAG
    GAGAGAAATC GCCAAATGAA AATTAACAAA TACAAACAGG TAGCCGGATC AGATCCCAGA
    CTGGAACAAG ATTACCATGC AAAGTATCGA TCGGGATGGG ATCCTCATAG AGGGGCAGAT
    AATATTTCCA CTAAATCTTC GGACAGTGAC GTAAGTGATA TATCTGCGGT TTCCAGGACT
    AGTAGTGCTT CTCGTTTCAG CAGCACAAGC TACATGTCTG TCCAATCAGA ACGGCCAAGA
    GGAAACAAGA AAATCAGTGT CTTTACATCC AAAATGCAAA GCAGACAAAT GGGCATCTCA
    GGGAAGAACA TGACCAAGAG CACCAGCATC AGCGGAGACA TGTGCTCGCT GGAGAAGAAT
    GATGGCAGCC AGTCGGACAC CGCAGTGGGT GCCTTGGGCA CCAGCAGCAA GAAGCGACGC
    TCTAGCATTG GGGCCAAAAT GGTAGCTATC GTTGGTCTTT CCCGGAAGAG CCGCAGTGCT
    TCTCAGCTCA GCCAAACGGA AACCGGAGGT AAAAAGCTAC GGAGCACTGT CCAGAGAAGT
    ACAGAAACAG GCTTGGCTGT GGAAATGAGG AACTGGATGA CTCGACAAGC GAGCCGAGAA
    TCTACCGACG GCAGCATGAA CAGCTACAGC TCAGAAGGAA ATCTGATCTT CCCTGGTGTC
    CGCTTGGCCT CGGATAGCCA ATTCAGTGAT TTTCTGGATG GCCTTGGCCC TGCCCAGCTA
    GTGGGGCGCC AAACTCTGGC CACACCCGCC ATGGGTGACA TTCAGGTAGG AATGATGGAC
    AAAAAGGGAC AGCTGGAGGT GGAAATAATT CGGGCCCGTG GCCTTGTTGT AAAACCAGGT
    TCCAAGACAC TGCCAGCACC ATACGTAAAG GTGTATCTAT TAGATAATGG AGTCTGCATA
    GCCAAAAAGA AAACAAAAGT GGCAAGAAAA ACGCTGGAAC CCCTTTACCA GCAACTATTA
    TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT TTACAGATCA TTGTCTGGGG AGACTATGGC
    CGCATGGATC ACAAATCCTT TATGGGAGTG GCCCAGATAC TTTTAGATGA ACTGGAGCTA
    TCCAACATGG TGATCGGCTG GTTCAAACTC TTCCCCCCTT CCTCCCTAGT AGATCCAACC
    TTGGCCCCTC TGACAAGAAG AGCTTCCCAA TCATCTCTGG AAAGTTCAAC TGGACCTTCT
    TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857442.1
    CDS2343..6317
    Translation

    Target ORF information:

    RefSeq Version XM_025001674.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GGATTGTGAA GCAGCAGATA GACAGCCATA TCACAGATCC
    AGATCAACAG AACAGCGGCC TCTCCTAGAG CGGACCACCA CCCGCTCCAG ATCCACTGAA
    CGTCCTGATA CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACTGGAAG ATCTGCCCCT
    CCTTCACCTG CCTTATCGAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA AAGACGTGGA CGCCACAAGG AGAAGACATG CAGGTGCTAT GGATATAGAG
    GAGAGAAATC GCCAAATGAA AATTAACAAA TACAAACAGG TAGCCGGATC AGATCCCAGA
    CTGGAACAAG ATTACCATGC AAAGTATCGA TCGGGATGGG ATCCTCATAG AGGGGCAGAT
    AATATTTCCA CTAAATCTTC GGACAGTGAC GTAAGTGATA TATCTGCGGT TTCCAGGACT
    AGTAGTGCTT CTCGTTTCAG CAGCACAAGC TACATGTCTG TCCAATCAGA ACGGCCAAGA
    GGAAACAAGA AAATCAGTGT CTTTACATCC AAAATGCAAA GCAGACAAAT GGGCATCTCA
    GGGAAGAACA TGACCAAGAG CACCAGCATC AGCGGAGACA TGTGCTCGCT GGAGAAGAAT
    GATGGCAGCC AGTCGGACAC CGCAGTGGGT GCCTTGGGCA CCAGCAGCAA GAAGCGACGC
    TCTAGCATTG GGGCCAAAAT GGTAGCTATC GTTGGTCTTT CCCGGAAGAG CCGCAGTGCT
    TCTCAGCTCA GCCAAACGGA AACCGGAGGT AAAAAGCTAC GGAGCACTGT CCAGAGAAGT
    ACAGAAACAG GCTTGGCTGT GGAAATGAGG AACTGGATGA CTCGACAAGC GAGCCGAGAA
    TCTACCGACG GCAGCATGAA CAGCTACAGC TCAGAAGGAA ATCTGATCTT CCCTGGTGTC
    CGCTTGGCCT CGGATAGCCA ATTCAGTGAT TTTCTGGATG GCCTTGGCCC TGCCCAGCTA
    GTGGGGCGCC AAACTCTGGC CACACCCGCC ATGGGTGACA TTCAGGTAGG AATGATGGAC
    AAAAAGGGAC AGCTGGAGGT GGAAATAATT CGGGCCCGTG GCCTTGTTGT AAAACCAGGT
    TCCAAGACAC TGCCAGCACC ATACGTAAAG GTGTATCTAT TAGATAATGG AGTCTGCATA
    GCCAAAAAGA AAACAAAAGT GGCAAGAAAA ACGCTGGAAC CCCTTTACCA GCAACTATTA
    TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT TTACAGATCA TTGTCTGGGG AGACTATGGC
    CGCATGGATC ACAAATCCTT TATGGGAGTG GCCCAGATAC TTTTAGATGA ACTGGAGCTA
    TCCAACATGG TGATCGGCTG GTTCAAACTC TTCCCCCCTT CCTCCCTAGT AGATCCAACC
    TTGGCCCCTC TGACAAGAAG AGCTTCCCAA TCATCTCTGG AAAGTTCAAC TGGACCTTCT
    TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222380
    Clone ID Related Accession (Same CDS sequence) XM_025001675.1
    Accession Version XM_025001675.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA ACAATGGAGT CAAGGAGATA GAACCTTATG AAGAAGACGT GGACGCCACA
    AGGAGAAGAC ATGCAGGTGC TATGGATATA GAGGAGAGAA ATCGCCAAAT GAAAATTAAC
    AAATACAAAC AGGTAGCCGG ATCAGATCCC AGACTGGAAC AAGATTACCA TGCAAAGTAT
    CGATCGGGAT GGGATCCTCA TAGAGGGGCA GATAATATTT CCACTAAATC TTCGGACAGT
    GACGTAAGTG ATATATCTGC GGTTTCCAGG ACTAGTAGTG CTTCTCGTTT CAGCAGCACA
    AGCTACATGT CTGTCCAATC AGAACGGCCA AGAGGAAACA AGAAAATCAG TGTCTTTACA
    TCCAAAATGC AAAGCAGACA AATGGGCATC TCAGGGAAGA ACATGACCAA GAGCACCAGC
    ATCAGCGGAG ACATGTGCTC GCTGGAGAAG AATGATGGCA GCCAGTCGGA CACCGCAGTG
    GGTGCCTTGG GCACCAGCAG CAAGAAGCGA CGCTCTAGCA TTGGGGCCAA AATGGTAGCT
    ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT GCTTCTCAGC TCAGCCAAAC GGAAACCGGA
    GGTAAAAAGC TACGGAGCAC TGTCCAGAGA AGTACAGAAA CAGGCTTGGC TGTGGAAATG
    AGGAACTGGA TGACTCGACA AGCGAGCCGA GAATCTACCG ACGGCAGCAT GAACAGCTAC
    AGCTCAGAAG GAAATCTGAT CTTCCCTGGT GTCCGCTTGG CCTCGGATAG CCAATTCAGT
    GATTTTCTGG ATGGCCTTGG CCCTGCCCAG CTAGTGGGGC GCCAAACTCT GGCCACACCC
    GCCATGGGTG ACATTCAGGT AGGAATGATG GACAAAAAGG GACAGCTGGA GGTGGAAATA
    ATTCGGGCCC GTGGCCTTGT TGTAAAACCA GGTTCCAAGA CACTGCCAGC ACCATACGTA
    AAGGTGTATC TATTAGATAA TGGAGTCTGC ATAGCCAAAA AGAAAACAAA AGTGGCAAGA
    AAAACGCTGG AACCCCTTTA CCAGCAACTA TTATCTTTTG AAGAAAGTCC CCAAGGAAAG
    GTTTTACAGA TCATTGTCTG GGGAGACTAT GGCCGCATGG ATCACAAATC CTTTATGGGA
    GTGGCCCAGA TACTTTTAGA TGAACTGGAG CTATCCAACA TGGTGATCGG CTGGTTCAAA
    CTCTTCCCCC CTTCCTCCCT AGTAGATCCA ACCTTGGCCC CTCTGACAAG AAGAGCTTCC
    CAATCATCTC TGGAAAGTTC AACTGGACCT TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857443.1
    CDS2343..6170
    Translation

    Target ORF information:

    RefSeq Version XM_025001675.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001675.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG GTCTCATCCT CGTACTGGGT CTGTCCAAAC AAGCCCATCA
    AGTACTCCAG TGGCAGGACG AAGGGGCCGA CAACTCCCAC AGCTTCCACC AAAGGGAACA
    TTGGAGAGAA ACAATGGAGT CAAGGAGATA GAACCTTATG AAGAAGACGT GGACGCCACA
    AGGAGAAGAC ATGCAGGTGC TATGGATATA GAGGAGAGAA ATCGCCAAAT GAAAATTAAC
    AAATACAAAC AGGTAGCCGG ATCAGATCCC AGACTGGAAC AAGATTACCA TGCAAAGTAT
    CGATCGGGAT GGGATCCTCA TAGAGGGGCA GATAATATTT CCACTAAATC TTCGGACAGT
    GACGTAAGTG ATATATCTGC GGTTTCCAGG ACTAGTAGTG CTTCTCGTTT CAGCAGCACA
    AGCTACATGT CTGTCCAATC AGAACGGCCA AGAGGAAACA AGAAAATCAG TGTCTTTACA
    TCCAAAATGC AAAGCAGACA AATGGGCATC TCAGGGAAGA ACATGACCAA GAGCACCAGC
    ATCAGCGGAG ACATGTGCTC GCTGGAGAAG AATGATGGCA GCCAGTCGGA CACCGCAGTG
    GGTGCCTTGG GCACCAGCAG CAAGAAGCGA CGCTCTAGCA TTGGGGCCAA AATGGTAGCT
    ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT GCTTCTCAGC TCAGCCAAAC GGAAACCGGA
    GGTAAAAAGC TACGGAGCAC TGTCCAGAGA AGTACAGAAA CAGGCTTGGC TGTGGAAATG
    AGGAACTGGA TGACTCGACA AGCGAGCCGA GAATCTACCG ACGGCAGCAT GAACAGCTAC
    AGCTCAGAAG GAAATCTGAT CTTCCCTGGT GTCCGCTTGG CCTCGGATAG CCAATTCAGT
    GATTTTCTGG ATGGCCTTGG CCCTGCCCAG CTAGTGGGGC GCCAAACTCT GGCCACACCC
    GCCATGGGTG ACATTCAGGT AGGAATGATG GACAAAAAGG GACAGCTGGA GGTGGAAATA
    ATTCGGGCCC GTGGCCTTGT TGTAAAACCA GGTTCCAAGA CACTGCCAGC ACCATACGTA
    AAGGTGTATC TATTAGATAA TGGAGTCTGC ATAGCCAAAA AGAAAACAAA AGTGGCAAGA
    AAAACGCTGG AACCCCTTTA CCAGCAACTA TTATCTTTTG AAGAAAGTCC CCAAGGAAAG
    GTTTTACAGA TCATTGTCTG GGGAGACTAT GGCCGCATGG ATCACAAATC CTTTATGGGA
    GTGGCCCAGA TACTTTTAGA TGAACTGGAG CTATCCAACA TGGTGATCGG CTGGTTCAAA
    CTCTTCCCCC CTTCCTCCCT AGTAGATCCA ACCTTGGCCC CTCTGACAAG AAGAGCTTCC
    CAATCATCTC TGGAAAGTTC AACTGGACCT TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92550
    Clone ID Related Accession (Same CDS sequence) XM_015474495.1
    Accession Version XM_015474495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329981.1
    CDS104..4174
    Translation

    Target ORF information:

    RefSeq Version XM_015474495.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAACC
    GGAGGTAAAA AGCTACGGAG CACTGTCCAG AGAAGTACAG AAACAGGCTT GGCTGTGGAA
    ATGAGGAACT GGATGACTCG ACAAGCGAGC CGAGAATCTA CCGACGGCAG CATGAACAGC
    TACAGCTCAG AAGGAAATCT GATCTTCCCT GGTGTCCGCT TGGCCTCGGA TAGCCAATTC
    AGTGATTTTC TGGATGGCCT TGGCCCTGCC CAGCTAGTGG GGCGCCAAAC TCTGGCCACA
    CCCGCCATGG GTGACATTCA GGTAGGAATG ATGGACAAAA AGGGACAGCT GGAGGTGGAA
    ATAATTCGGG CCCGTGGCCT TGTTGTAAAA CCAGGTTCCA AGACACTGCC AGCACCATAC
    GTAAAGGTGT ATCTATTAGA TAATGGAGTC TGCATAGCCA AAAAGAAAAC AAAAGTGGCA
    AGAAAAACGC TGGAACCCCT TTACCAGCAA CTATTATCTT TTGAAGAAAG TCCCCAAGGA
    AAGGTTTTAC AGATCATTGT CTGGGGAGAC TATGGCCGCA TGGATCACAA ATCCTTTATG
    GGAGTGGCCC AGATACTTTT AGATGAACTG GAGCTATCCA ACATGGTGAT CGGCTGGTTC
    AAACTCTTCC CCCCTTCCTC CCTAGTAGAT CCAACCTTGG CCCCTCTGAC AAGAAGAGCT
    TCCCAATCAT CTCTGGAAAG TTCAACTGGA CCTTCTTACT CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92551
    Clone ID Related Accession (Same CDS sequence) XM_015474496.1
    Accession Version XM_015474496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGG
    GATTGTGAAG CAGCAGATAG ACAGCCATAT CACAGATCCA GATCAACAGA ACAGCGGCCT
    CTCCTAGAGC GGACCACCAC CCGCTCCAGA TCCACTGAAC GTCCTGATAC AAACCTCATG
    AGGTCGATGC CTTCATTAAT GACTGGAAGA TCTGCCCCTC CTTCACCTGC CTTATCGAGG
    TCTCATCCTC GTACTGGGTC TGTCCAAACA AGCCCATCAA GTACTCCAGT GGCAGGACGA
    AGGGGCCGAC AACTCCCACA GCTTCCACCA AAGGGAACAT TGGAGAGAAA AACCGGAGGT
    AAAAAGCTAC GGAGCACTGT CCAGAGAAGT ACAGAAACAG GCTTGGCTGT GGAAATGAGG
    AACTGGATGA CTCGACAAGC GAGCCGAGAA TCTACCGACG GCAGCATGAA CAGCTACAGC
    TCAGAAGGAA ATCTGATCTT CCCTGGTGTC CGCTTGGCCT CGGATAGCCA ATTCAGTGAT
    TTTCTGGATG GCCTTGGCCC TGCCCAGCTA GTGGGGCGCC AAACTCTGGC CACACCCGCC
    ATGGGTGACA TTCAGGTAGG AATGATGGAC AAAAAGGGAC AGCTGGAGGT GGAAATAATT
    CGGGCCCGTG GCCTTGTTGT AAAACCAGGT TCCAAGACAC TGCCAGCACC ATACGTAAAG
    GTGTATCTAT TAGATAATGG AGTCTGCATA GCCAAAAAGA AAACAAAAGT GGCAAGAAAA
    ACGCTGGAAC CCCTTTACCA GCAACTATTA TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT
    TTACAGATCA TTGTCTGGGG AGACTATGGC CGCATGGATC ACAAATCCTT TATGGGAGTG
    GCCCAGATAC TTTTAGATGA ACTGGAGCTA TCCAACATGG TGATCGGCTG GTTCAAACTC
    TTCCCCCCTT CCTCCCTAGT AGATCCAACC TTGGCCCCTC TGACAAGAAG AGCTTCCCAA
    TCATCTCTGG AAAGTTCAAC TGGACCTTCT TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329982.1
    CDS104..4108
    Translation

    Target ORF information:

    RefSeq Version XM_015474496.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGG
    GATTGTGAAG CAGCAGATAG ACAGCCATAT CACAGATCCA GATCAACAGA ACAGCGGCCT
    CTCCTAGAGC GGACCACCAC CCGCTCCAGA TCCACTGAAC GTCCTGATAC AAACCTCATG
    AGGTCGATGC CTTCATTAAT GACTGGAAGA TCTGCCCCTC CTTCACCTGC CTTATCGAGG
    TCTCATCCTC GTACTGGGTC TGTCCAAACA AGCCCATCAA GTACTCCAGT GGCAGGACGA
    AGGGGCCGAC AACTCCCACA GCTTCCACCA AAGGGAACAT TGGAGAGAAA AACCGGAGGT
    AAAAAGCTAC GGAGCACTGT CCAGAGAAGT ACAGAAACAG GCTTGGCTGT GGAAATGAGG
    AACTGGATGA CTCGACAAGC GAGCCGAGAA TCTACCGACG GCAGCATGAA CAGCTACAGC
    TCAGAAGGAA ATCTGATCTT CCCTGGTGTC CGCTTGGCCT CGGATAGCCA ATTCAGTGAT
    TTTCTGGATG GCCTTGGCCC TGCCCAGCTA GTGGGGCGCC AAACTCTGGC CACACCCGCC
    ATGGGTGACA TTCAGGTAGG AATGATGGAC AAAAAGGGAC AGCTGGAGGT GGAAATAATT
    CGGGCCCGTG GCCTTGTTGT AAAACCAGGT TCCAAGACAC TGCCAGCACC ATACGTAAAG
    GTGTATCTAT TAGATAATGG AGTCTGCATA GCCAAAAAGA AAACAAAAGT GGCAAGAAAA
    ACGCTGGAAC CCCTTTACCA GCAACTATTA TCTTTTGAAG AAAGTCCCCA AGGAAAGGTT
    TTACAGATCA TTGTCTGGGG AGACTATGGC CGCATGGATC ACAAATCCTT TATGGGAGTG
    GCCCAGATAC TTTTAGATGA ACTGGAGCTA TCCAACATGG TGATCGGCTG GTTCAAACTC
    TTCCCCCCTT CCTCCCTAGT AGATCCAACC TTGGCCCCTC TGACAAGAAG AGCTTCCCAA
    TCATCTCTGG AAAGTTCAAC TGGACCTTCT TACTCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92552
    Clone ID Related Accession (Same CDS sequence) XM_015474497.1
    Accession Version XM_015474497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA
    GACGTGGATC ACAACTGGTT GGACCATACG TCTTGGCATA GCAGTGAGGC ATCCCCAATG
    TCTTTGCACC CTGTGACGTG GCAGCCATCT AAAGATGGAG ATCGTTTAAT TGGTCGCATT
    TTATTAAATA AGCGTTTAAA AGATGGAAGT GTACCTCGAG ATTCAGGAGC AATGCTTGGC
    TTGAAGGTTG TAGGAGGAAA GATGACTGAA TCAGGTCGGC TCTGTGCATT TATAACCAAA
    GTTAAAAGAG GAAGTTTAGC TGATACTGTA GGACATCTTA GACCAGGTGA TGAAGTACTA
    GAGTGGAATG GAAGACTATT ACAAGGAGCC ACATTTGAAG AAGTATATAA CATCATCCTG
    GAATCCAAAC CTGAACCACA GGTGGAGCTT GTTGTTTCAA GGCCTATTGG AGATATACCT
    AGAATACCTG ACAGCACACA CACACAGCTG GAGTCCAGTT CTAGCTCATT CGAATCTCAA
    AAAATGGATC GTCCTTCTAT TTCTGTTACA TCTCCCATGA GTCCTGGCAT GTTGAGGGAT
    GTTCCACAGT TCTTATCTGG ACAACTTTCA ATAAAACTGT GGTTTGACAA GGTTGGTCAC
    CAATTAATAG TTACAATTTT GGGAGCAAAA GACCTCCCTT CCAGGGAAGA TGGGAGGCCA
    AGGAATCCTT ATGTTAAAAT TTACTTTCTT CCAGACAGAA GTGACAAAAA CAAGAGAAGA
    ACTAAAACAG TGAAGAAAAC TTTGGAACCC AAATGGAACC AAACATTCAT TTATTCTCCA
    GTCCACCGAA GAGAGTTTCG GGAACGGATG CTGGAGATTA CCCTTTGGGA TCAAGCTCGA
    GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA GAGATTTTAA TTGAATTAGA AACAGCATTA
    TTAGATGATG AGCCACATTG GTACAAACTT CAGACTCACG ATGTTTCTTC ATTGCCACTT
    CCCCATCCTT CACCATATAT GCCACGACGA CAACTCCATG GAGAGAGCCC AACACGGAGG
    TTGCAAAGAT CAAAGAGAAT AAGTGATAGT GAGGTCTCTG ATTATGACTG TGATGATGGA
    ATTGGTGTAG TATCAGATTA TCGACACAAT GGTCGGGATC TTCAAAGCTC AACATTGTCA
    GTGCCAGAAC AAGTAATGTC ATCCAACCAC TGTTCGCCGT CAGGGTCTCC ACACCGAGTA
    GATGTTATAG GAAGGACTAG ATCTTGGTCA CCCAGTGTCC CTCCTCCACA GAGTCGAAAT
    GTGGAGCAGG GACTTCGAGG GACACGCTCT GCTATAGGAC ATTATAATAC AATTAGCCGA
    ATGGATAGAC ACCGTGTCAT GGATGACCAT TATTCTCCAG ACAGAGACAG TCATTTTCTT
    ACTCTACCTC GCTCCAGATA CAGTCAGAGC GTTGAACATC ATCACAGGGA TGGCAGGGAT
    TGTGAAGCAG CAGATAGACA GCCATATCAC AGATCCAGAT CAACAGAACA GCGGCCTCTC
    CTAGAGCGGA CCACCACCCG CTCCAGATCC ACTGAACGTC CTGATACAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ATCGAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAGTGC TATGGATATA
    GAGGAGAGAA ATCGCCAAAT GAAAATTAAC AAATACAAAC AGGTAGCCGG ATCAGATCCC
    AGACTGGAAC AAGATTACCA TGCAAAGTAT CGATCGGGAT GGGATCCTCA TAGAGGGGCA
    GATAATATTT CCACTAAATC TTCGGACAGT GACGTAAGTG ATATATCTGC GGTTTCCAGG
    ACTAGTAGTG CTTCTCGTTT CAGCAGCACA AGCTACATGT CTGTCCAATC AGAACGGCCA
    AGAGGAAACA AGAAAATCAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329983.1
    CDS104..4501
    Translation

    Target ORF information:

    RefSeq Version XM_015474497.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA
    GACGTGGATC ACAACTGGTT GGACCATACG TCTTGGCATA GCAGTGAGGC ATCCCCAATG
    TCTTTGCACC CTGTGACGTG GCAGCCATCT AAAGATGGAG ATCGTTTAAT TGGTCGCATT
    TTATTAAATA AGCGTTTAAA AGATGGAAGT GTACCTCGAG ATTCAGGAGC AATGCTTGGC
    TTGAAGGTTG TAGGAGGAAA GATGACTGAA TCAGGTCGGC TCTGTGCATT TATAACCAAA
    GTTAAAAGAG GAAGTTTAGC TGATACTGTA GGACATCTTA GACCAGGTGA TGAAGTACTA
    GAGTGGAATG GAAGACTATT ACAAGGAGCC ACATTTGAAG AAGTATATAA CATCATCCTG
    GAATCCAAAC CTGAACCACA GGTGGAGCTT GTTGTTTCAA GGCCTATTGG AGATATACCT
    AGAATACCTG ACAGCACACA CACACAGCTG GAGTCCAGTT CTAGCTCATT CGAATCTCAA
    AAAATGGATC GTCCTTCTAT TTCTGTTACA TCTCCCATGA GTCCTGGCAT GTTGAGGGAT
    GTTCCACAGT TCTTATCTGG ACAACTTTCA ATAAAACTGT GGTTTGACAA GGTTGGTCAC
    CAATTAATAG TTACAATTTT GGGAGCAAAA GACCTCCCTT CCAGGGAAGA TGGGAGGCCA
    AGGAATCCTT ATGTTAAAAT TTACTTTCTT CCAGACAGAA GTGACAAAAA CAAGAGAAGA
    ACTAAAACAG TGAAGAAAAC TTTGGAACCC AAATGGAACC AAACATTCAT TTATTCTCCA
    GTCCACCGAA GAGAGTTTCG GGAACGGATG CTGGAGATTA CCCTTTGGGA TCAAGCTCGA
    GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA GAGATTTTAA TTGAATTAGA AACAGCATTA
    TTAGATGATG AGCCACATTG GTACAAACTT CAGACTCACG ATGTTTCTTC ATTGCCACTT
    CCCCATCCTT CACCATATAT GCCACGACGA CAACTCCATG GAGAGAGCCC AACACGGAGG
    TTGCAAAGAT CAAAGAGAAT AAGTGATAGT GAGGTCTCTG ATTATGACTG TGATGATGGA
    ATTGGTGTAG TATCAGATTA TCGACACAAT GGTCGGGATC TTCAAAGCTC AACATTGTCA
    GTGCCAGAAC AAGTAATGTC ATCCAACCAC TGTTCGCCGT CAGGGTCTCC ACACCGAGTA
    GATGTTATAG GAAGGACTAG ATCTTGGTCA CCCAGTGTCC CTCCTCCACA GAGTCGAAAT
    GTGGAGCAGG GACTTCGAGG GACACGCTCT GCTATAGGAC ATTATAATAC AATTAGCCGA
    ATGGATAGAC ACCGTGTCAT GGATGACCAT TATTCTCCAG ACAGAGACAG TCATTTTCTT
    ACTCTACCTC GCTCCAGATA CAGTCAGAGC GTTGAACATC ATCACAGGGA TGGCAGGGAT
    TGTGAAGCAG CAGATAGACA GCCATATCAC AGATCCAGAT CAACAGAACA GCGGCCTCTC
    CTAGAGCGGA CCACCACCCG CTCCAGATCC ACTGAACGTC CTGATACAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ATCGAGGTCT
    CATCCTCGTA CTGGGTCTGT CCAAACAAGC CCATCAAGTA CTCCAGTGGC AGGACGAAGG
    GGCCGACAAC TCCCACAGCT TCCACCAAAG GGAACATTGG AGAGAAGTGC TATGGATATA
    GAGGAGAGAA ATCGCCAAAT GAAAATTAAC AAATACAAAC AGGTAGCCGG ATCAGATCCC
    AGACTGGAAC AAGATTACCA TGCAAAGTAT CGATCGGGAT GGGATCCTCA TAGAGGGGCA
    GATAATATTT CCACTAAATC TTCGGACAGT GACGTAAGTG ATATATCTGC GGTTTCCAGG
    ACTAGTAGTG CTTCTCGTTT CAGCAGCACA AGCTACATGT CTGTCCAATC AGAACGGCCA
    AGAGGAAACA AGAAAATCAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26046
    Clone ID Related Accession (Same CDS sequence) XM_005215574.4 , XM_005215574.3
    Accession Version XM_005215574.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215574.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GGGATTGTGA AGCAGCAGAT
    AGACAGCCAT ATCACAGATC CAGATCAACA GAACAGCGGC CTCTCCTAGA GCGGACCACC
    ACCCGCTCCA GATCCACTGA ACGTCCTGAT ACAAACCTCA TGAGGTCGAT GCCTTCATTA
    ATGACTGGAA GATCTGCCCC TCCTTCACCT GCCTTATCGA GGTCTCATCC TCGTACTGGG
    TCTGTCCAAA CAAGCCCATC AAGTACTCCA GTGGCAGGAC GAAGGGGCCG ACAACTCCCA
    CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA AAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215631.1
    CDS2343..5909
    Translation

    Target ORF information:

    RefSeq Version XM_005215574.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215574.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GGGATTGTGA AGCAGCAGAT
    AGACAGCCAT ATCACAGATC CAGATCAACA GAACAGCGGC CTCTCCTAGA GCGGACCACC
    ACCCGCTCCA GATCCACTGA ACGTCCTGAT ACAAACCTCA TGAGGTCGAT GCCTTCATTA
    ATGACTGGAA GATCTGCCCC TCCTTCACCT GCCTTATCGA GGTCTCATCC TCGTACTGGG
    TCTGTCCAAA CAAGCCCATC AAGTACTCCA GTGGCAGGAC GAAGGGGCCG ACAACTCCCA
    CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA AAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26046
    Clone ID Related Accession (Same CDS sequence) XM_005215574.4 , XM_005215574.3
    Accession Version XM_005215574.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GGGATTGTGA AGCAGCAGAT
    AGACAGCCAT ATCACAGATC CAGATCAACA GAACAGCGGC CTCTCCTAGA GCGGACCACC
    ACCCGCTCCA GATCCACTGA ACGTCCTGAT ACAAACCTCA TGAGGTCGAT GCCTTCATTA
    ATGACTGGAA GATCTGCCCC TCCTTCACCT GCCTTATCGA GGTCTCATCC TCGTACTGGG
    TCTGTCCAAA CAAGCCCATC AAGTACTCCA GTGGCAGGAC GAAGGGGCCG ACAACTCCCA
    CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA AAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215631.1
    CDS609..4175
    Translation

    Target ORF information:

    RefSeq Version XM_005215574.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215574.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG GACAGTCAAA AGGGAAAAAG AAAAACTAGT
    GAGCAGGCAG TTTTGTCGGA CTCTAACACC AGGTCTGAGA GACAAAAGAA AATGATGTAC
    TTTGGAGGCC ACTCTTTGGA AGAGGATTTG GAATGGTCTG AGCCTCAGAT TAAGGACTCT
    GGGGTAGATA CGTGTAGTAG CACAACCCTT AATGAGGAGC ATAGCCATAG TGATAAGCAC
    CCTGTGACGT GGCAGCCATC TAAAGATGGA GATCGTTTAA TTGGTCGCAT TTTATTAAAT
    AAGCGTTTAA AAGATGGAAG TGTACCTCGA GATTCAGGAG CAATGCTTGG CTTGAAGGTT
    GTAGGAGGAA AGATGACTGA ATCAGGTCGG CTCTGTGCAT TTATAACCAA AGTTAAAAGA
    GGAAGTTTAG CTGATACTGT AGGACATCTT AGACCAGGTG ATGAAGTACT AGAGTGGAAT
    GGAAGACTAT TACAAGGAGC CACATTTGAA GAAGTATATA ACATCATCCT GGAATCCAAA
    CCTGAACCAC AGGTGGAGCT TGTTGTTTCA AGGCCTATTG GAGATATACC TAGAATACCT
    GACAGCACAC ACACACAGCT GGAGTCCAGT TCTAGCTCAT TCGAATCTCA AAAAATGGAT
    CGTCCTTCTA TTTCTGTTAC ATCTCCCATG AGTCCTGGCA TGTTGAGGGA TGTTCCACAG
    TTCTTATCTG GACAACTTTC AATAAAACTG TGGTTTGACA AGGTTGGTCA CCAATTAATA
    GTTACAATTT TGGGAGCAAA AGACCTCCCT TCCAGGGAAG ATGGGAGGCC AAGGAATCCT
    TATGTTAAAA TTTACTTTCT TCCAGACAGA AGTGACAAAA ACAAGAGAAG AACTAAAACA
    GTGAAGAAAA CTTTGGAACC CAAATGGAAC CAAACATTCA TTTATTCTCC AGTCCACCGA
    AGAGAGTTTC GGGAACGGAT GCTGGAGATT ACCCTTTGGG ATCAAGCTCG AGTTCGAGAG
    GAAGAAAGTG AATTTTTAGG AGAGATTTTA ATTGAATTAG AAACAGCATT ATTAGATGAT
    GAGCCACATT GGTACAAACT TCAGACTCAC GATGTTTCTT CATTGCCACT TCCCCATCCT
    TCACCATATA TGCCACGACG ACAACTCCAT GGAGAGAGCC CAACACGGAG GTTGCAAAGA
    TCAAAGAGAA TAAGTGATAG TGAGGTCTCT GATTATGACT GTGATGATGG AATTGGTGTA
    GTATCAGATT ATCGACACAA TGGTCGGGAT CTTCAAAGCT CAACATTGTC AGTGCCAGAA
    CAAGTAATGT CATCCAACCA CTGTTCGCCG TCAGGGTCTC CACACCGAGT AGATGTTATA
    GGAAGGACTA GATCTTGGTC ACCCAGTGTC CCTCCTCCAC AGAGTCGAAA TGTGGAGCAG
    GGACTTCGAG GGACACGCTC TGCTATAGGA CATTATAATA CAATTAGCCG AATGGATAGA
    CACCGTGTCA TGGATGACCA TTATTCTCCA GACAGAGACA GGGATTGTGA AGCAGCAGAT
    AGACAGCCAT ATCACAGATC CAGATCAACA GAACAGCGGC CTCTCCTAGA GCGGACCACC
    ACCCGCTCCA GATCCACTGA ACGTCCTGAT ACAAACCTCA TGAGGTCGAT GCCTTCATTA
    ATGACTGGAA GATCTGCCCC TCCTTCACCT GCCTTATCGA GGTCTCATCC TCGTACTGGG
    TCTGTCCAAA CAAGCCCATC AAGTACTCCA GTGGCAGGAC GAAGGGGCCG ACAACTCCCA
    CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA AAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26047
    Clone ID Related Accession (Same CDS sequence) XM_005215575.3 , XM_005215575.4
    Accession Version XM_005215575.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215632.1
    CDS606..4097
    Translation

    Target ORF information:

    RefSeq Version XM_005215575.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215575.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26047
    Clone ID Related Accession (Same CDS sequence) XM_005215575.3 , XM_005215575.4
    Accession Version XM_005215575.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215632.1
    CDS2342..5833
    Translation

    Target ORF information:

    RefSeq Version XM_005215575.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215575.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAATTTG AGACATTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
    TTTTCATCCC CACCAAATAT TTTACAAAAT GAGCTTTTTG GACAAACGCT GAACAATGCA
    AGGAAAAGAA GTCCATCAGT GTCCAGAGAT CAGAACAGAA GATACGACCA AAGGGAAGAA
    AGAGAAGAAT ATTCACAGTA TGCTACTTCG GACAGCGCAA TGCCTAGATC TCCATCAGAT
    TATGCTGATA GGAGGTCTCA ACCTGAACCT CAGTTTTATG AAGAGTCTGA TCATATAAAT
    TATAGGGACT CCAACAGGAG AAGTCATAGA CATTCCAAAG AATATATTGT AGATGACAAG
    GATGTGGAGA GTAGAGATGA ATATGAAAGA CAGAGGAGAG AGGAGGAATA CCAGGCGCGC
    TATCGAAGTG ATCCGAATTT GGCCCGTTAT CCAGTCAAGC CACAGCCCTA TGAAGAACAA
    ATGCGTATCC ATGCTGAAGT GTCACGAGCC AGGCATGAGA GAAGGCACAG TGATGTTTCT
    TTGGCAAATG CTGAATTGGA AGACTCCAGG ATTTCTATGC TAAGGATGGA ACGACCATCA
    AGGCAAAGGT CTATATCTGA ACGTAGAGCT GCCATGGAAA ATCAGCGATC TTATTCAATG
    GAAAGAACTC GAGAGGCTCA GGGACCAAGT TCTTATCCAC AAAGGACCAC AAACCATAGT
    CCTCCTACCC CCCGGAGGAG TCCAATACCC ATTGATAGAC CAGACGTGAG ACGTACTGAC
    TCACTAAGGA AACAACACCA CCTAGATCCT AGTTCTGCTG TAAGGAAAAC GAAACGGGAA
    AAAATGGAAA CAATGTTAAG GAATGATTCT CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA
    CCTCCACCAC CAAAGCCTCA TAAATCAAAG AAAGGAGGTA AAATGCGCCA GGTTTCATTG
    AGCAGTTCAG AGGAGGAATT AGCTTCTACA CCTGAATATA CCAGTTGCGA TGATGTTGAG
    ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA GACGTGGATC ACAACTGGTT GGACCATACG
    TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCACC CTGTGACGTG GCAGCCATCT
    AAAGATGGAG ATCGTTTAAT TGGTCGCATT TTATTAAATA AGCGTTTAAA AGATGGAAGT
    GTACCTCGAG ATTCAGGAGC AATGCTTGGC TTGAAGGTTG TAGGAGGAAA GATGACTGAA
    TCAGGTCGGC TCTGTGCATT TATAACCAAA GTTAAAAGAG GAAGTTTAGC TGATACTGTA
    GGACATCTTA GACCAGGTGA TGAAGTACTA GAGTGGAATG GAAGACTATT ACAAGGAGCC
    ACATTTGAAG AAGTATATAA CATCATCCTG GAATCCAAAC CTGAACCACA GGTGGAGCTT
    GTTGTTTCAA GGCCTATTGG AGATATACCT AGAATACCTG ACAGCACACA CACACAGCTG
    GAGTCCAGTT CTAGCTCATT CGAATCTCAA AAAATGGATC GTCCTTCTAT TTCTGTTACA
    TCTCCCATGA GTCCTGGCAT GTTGAGGGAT GTTCCACAGT TCTTATCTGG ACAACTTTCA
    ATAAAACTGT GGTTTGACAA GGTTGGTCAC CAATTAATAG TTACAATTTT GGGAGCAAAA
    GACCTCCCTT CCAGGGAAGA TGGGAGGCCA AGGAATCCTT ATGTTAAAAT TTACTTTCTT
    CCAGACAGAA GTGACAAAAA CAAGAGAAGA ACTAAAACAG TGAAGAAAAC TTTGGAACCC
    AAATGGAACC AAACATTCAT TTATTCTCCA GTCCACCGAA GAGAGTTTCG GGAACGGATG
    CTGGAGATTA CCCTTTGGGA TCAAGCTCGA GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA
    GAGATTTTAA TTGAATTAGA AACAGCATTA TTAGATGATG AGCCACATTG GTACAAACTT
    CAGACTCACG ATGTTTCTTC ATTGCCACTT CCCCATCCTT CACCATATAT GCCACGACGA
    CAACTCCATG GAGAGAGCCC AACACGGAGG TTGCAAAGAT CAAAGAGAAT AAGTGATAGT
    GAGGTCTCTG ATTATGACTG TGATGATGGA ATTGGTGTAG TATCAGATTA TCGACACAAT
    GGTCGGGATC TTCAAAGCTC AACATTGTCA GTGCCAGAAC AAGTAATGTC ATCCAACCAC
    TGTTCGCCGT CAGGGTCTCC ACACCGAGTA GATGTTATAG GAAGGACTAG ATCTTGGTCA
    CCCAGTGTCC CTCCTCCACA GAGTCGAAAT GTGGAGCAGG GACTTCGAGG GACACGCTCT
    GCTATAGGAC ATTATAATAC AATTAGCCGA ATGGATAGAC ACCGTGTCAT GGATGACCAT
    TATTCTCCAG ACAGAGACAG TCATTTTCTT ACTCTACCTC GCTCCAGATA CAGTCAGAGC
    GTTGAACATC ATCACAGGGA TGGCAGGGAT TGTGAAGCAG CAGATAGACA GCCATATCAC
    AGATCCAGAT CAACAGAACA GCGGCCTCTC CTAGAGCGGA CCACCACCCG CTCCAGATCC
    ACTGAACGTC CTGATACAAA CCTCATGAGG TCGATGCCTT CATTAATGAC TGGAAGATCT
    GCCCCTCCTT CACCTGCCTT ATCGAGGTCT CATCCTCGTA CTGGGTCTGT CCAAACAAGC
    CCATCAAGTA CTCCAGTGGC AGGACGAAGG GGCCGACAAC TCCCACAGCT TCCACCAAAG
    GGAACATTGG AGAGAAAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222361
    Clone ID Related Accession (Same CDS sequence) XM_025001653.1
    Accession Version XM_025001653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGTCATT TTCTTACTCT ACCTCGCTCC AGATACAGTC AGAGCGTTGA ACATCATCAC
    AGGGATGGCA GGGATTGTGA AGCAGCAGAT AGACAGCCAT ATCACAGATC CAGATCAACA
    GAACAGCGGC CTCTCCTAGA GCGGACCACC ACCCGCTCCA GATCCACTGA ACGTCCTGAT
    ACAAACCTCA TGAGGTCGAT GCCTTCATTA ATGACTGGAA GATCTGCCCC TCCTTCACCT
    GCCTTATCGA GGTCTCATCC TCGTACTGGG TCTGTCCAAA CAAGCCCATC AAGTACTCCA
    GTGGCAGGAC GAAGGGGCCG ACAACTCCCA CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA
    AACAATGGAG TCAAGGAGAT AGAACCTTAT GAAGGTGCTA TGGATATAGA GGAGAGAAAT
    CGCCAAATGA AAATTAACAA ATACAAACAG GTAGCCGGAT CAGATCCCAG ACTGGAACAA
    GATTACCATG CAAAGTATCG ATCGGGATGG GATCCTCATA GAGGGGCAGA TAATATTTCC
    ACTAAATCTT CGGACAGTGA CGTAAGTGAT ATATCTGCGG TTTCCAGGAC TAGTAGTGCT
    TCTCGTTTCA GCAGCACAAG CTACATGTCT GTCCAATCAG AACGGCCAAG AGGAAACAAG
    AAAATCAGGC CAAAGGGACT AGAAGAGGGA GGGAAAGACG GGGATAAGCA TGAAGAGATA
    GTTCATGCGG AGGAAGAGGT AAAGGAAGAA AGAACTAATG AGAATGAGAA AGGGAAAAAC
    ATTACAAACA TGTGTAATAA GGAGACAAAC AGAGAATCAG GAGATGAGGA AAAAACACCA
    GATATACCTG AGCAAGGGAA AGAAAAAGAA CAGTGGAATA AAGAGGATTT GCAAAAGCAA
    TTCTCACAAG ATGATGACAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857421.1
    CDS373..5550
    Translation

    Target ORF information:

    RefSeq Version XM_025001653.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGTCATT TTCTTACTCT ACCTCGCTCC AGATACAGTC AGAGCGTTGA ACATCATCAC
    AGGGATGGCA GGGATTGTGA AGCAGCAGAT AGACAGCCAT ATCACAGATC CAGATCAACA
    GAACAGCGGC CTCTCCTAGA GCGGACCACC ACCCGCTCCA GATCCACTGA ACGTCCTGAT
    ACAAACCTCA TGAGGTCGAT GCCTTCATTA ATGACTGGAA GATCTGCCCC TCCTTCACCT
    GCCTTATCGA GGTCTCATCC TCGTACTGGG TCTGTCCAAA CAAGCCCATC AAGTACTCCA
    GTGGCAGGAC GAAGGGGCCG ACAACTCCCA CAGCTTCCAC CAAAGGGAAC ATTGGAGAGA
    AACAATGGAG TCAAGGAGAT AGAACCTTAT GAAGGTGCTA TGGATATAGA GGAGAGAAAT
    CGCCAAATGA AAATTAACAA ATACAAACAG GTAGCCGGAT CAGATCCCAG ACTGGAACAA
    GATTACCATG CAAAGTATCG ATCGGGATGG GATCCTCATA GAGGGGCAGA TAATATTTCC
    ACTAAATCTT CGGACAGTGA CGTAAGTGAT ATATCTGCGG TTTCCAGGAC TAGTAGTGCT
    TCTCGTTTCA GCAGCACAAG CTACATGTCT GTCCAATCAG AACGGCCAAG AGGAAACAAG
    AAAATCAGGC CAAAGGGACT AGAAGAGGGA GGGAAAGACG GGGATAAGCA TGAAGAGATA
    GTTCATGCGG AGGAAGAGGT AAAGGAAGAA AGAACTAATG AGAATGAGAA AGGGAAAAAC
    ATTACAAACA TGTGTAATAA GGAGACAAAC AGAGAATCAG GAGATGAGGA AAAAACACCA
    GATATACCTG AGCAAGGGAA AGAAAAAGAA CAGTGGAATA AAGAGGATTT GCAAAAGCAA
    TTCTCACAAG ATGATGACAG TGTCTTTACA TCCAAAATGC AAAGCAGACA AATGGGCATC
    TCAGGGAAGA ACATGACCAA GAGCACCAGC ATCAGCGGAG ACATGTGCTC GCTGGAGAAG
    AATGATGGCA GCCAGTCGGA CACCGCAGTG GGTGCCTTGG GCACCAGCAG CAAGAAGCGA
    CGCTCTAGCA TTGGGGCCAA AATGGTAGCT ATCGTTGGTC TTTCCCGGAA GAGCCGCAGT
    GCTTCTCAGC TCAGCCAAAC GGAAACCGGA GGTAAAAAGC TACGGAGCAC TGTCCAGAGA
    AGTACAGAAA CAGGCTTGGC TGTGGAAATG AGGAACTGGA TGACTCGACA AGCGAGCCGA
    GAATCTACCG ACGGCAGCAT GAACAGCTAC AGCTCAGAAG GAAATCTGAT CTTCCCTGGT
    GTCCGCTTGG CCTCGGATAG CCAATTCAGT GATTTTCTGG ATGGCCTTGG CCCTGCCCAG
    CTAGTGGGGC GCCAAACTCT GGCCACACCC GCCATGGGTG ACATTCAGGT AGGAATGATG
    GACAAAAAGG GACAGCTGGA GGTGGAAATA ATTCGGGCCC GTGGCCTTGT TGTAAAACCA
    GGTTCCAAGA CACTGCCAGC ACCATACGTA AAGGTGTATC TATTAGATAA TGGAGTCTGC
    ATAGCCAAAA AGAAAACAAA AGTGGCAAGA AAAACGCTGG AACCCCTTTA CCAGCAACTA
    TTATCTTTTG AAGAAAGTCC CCAAGGAAAG GTTTTACAGA TCATTGTCTG GGGAGACTAT
    GGCCGCATGG ATCACAAATC CTTTATGGGA GTGGCCCAGA TACTTTTAGA TGAACTGGAG
    CTATCCAACA TGGTGATCGG CTGGTTCAAA CTCTTCCCCC CTTCCTCCCT AGTAGATCCA
    ACCTTGGCCC CTCTGACAAG AAGAGCTTCC CAATCATCTC TGGAAAGTTC AACTGGACCT
    TCTTACTCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222362
    Clone ID Related Accession (Same CDS sequence) XM_025001654.1
    Accession Version XM_025001654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAACAAT
    GGAGTCAAGG AGATAGAACC TTATGAAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AAACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857422.1
    CDS373..5484
    Translation

    Target ORF information:

    RefSeq Version XM_025001654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAACAAT
    GGAGTCAAGG AGATAGAACC TTATGAAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AAACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222363
    Clone ID Related Accession (Same CDS sequence) XM_025001656.1
    Accession Version XM_025001656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAACA ATGGAGTCAA GGAGATAGAA CCTTATGAAG GTGCTATGGA TATAGAGGAG
    AGAAATCGCC AAATGAAAAT TAACAAATAC AAACAGGTAG CCGGATCAGA TCCCAGACTG
    GAACAAGATT ACCATGCAAA GTATCGATCG GGATGGGATC CTCATAGAGG GGCAGATAAT
    ATTTCCACTA AATCTTCGGA CAGTGACGTA AGTGATATAT CTGCGGTTTC CAGGACTAGT
    AGTGCTTCTC GTTTCAGCAG CACAAGCTAC ATGTCTGTCC AATCAGAACG GCCAAGAGGA
    AACAAGAAAA TCAGGCCAAA GGGACTAGAA GAGGGAGGGA AAGACGGGGA TAAGCATGAA
    GAGATAGTTC ATGCGGAGGA AGAGGTAAAG GAAGAAAGAA CTAATGAGAA TGAGAAAGGG
    AAAAACATTA CAAACATGTG TAATAAGGAG ACAAACAGAG AATCAGGAGA TGAGGAAAAA
    ACACCAGATA TACCTGAGCA AGGGAAAGAA AAAGAACAGT GGAATAAAGA GGATTTGCAA
    AAGCAATTCT CACAAGATGA TGACAGTGTC TTTACATCCA AAATGCAAAG CAGACAAATG
    GGCATCTCAG GGAAGAACAT GACCAAGAGC ACCAGCATCA GCGGAGACAT GTGCTCGCTG
    GAGAAGAATG ATGGCAGCCA GTCGGACACC GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG
    AAGCGACGCT CTAGCATTGG GGCCAAAATG GTAGCTATCG TTGGTCTTTC CCGGAAGAGC
    CGCAGTGCTT CTCAGCTCAG CCAAACGGAA ACCGGAGGTA AAAAGCTACG GAGCACTGTC
    CAGAGAAGTA CAGAAACAGG CTTGGCTGTG GAAATGAGGA ACTGGATGAC TCGACAAGCG
    AGCCGAGAAT CTACCGACGG CAGCATGAAC AGCTACAGCT CAGAAGGAAA TCTGATCTTC
    CCTGGTGTCC GCTTGGCCTC GGATAGCCAA TTCAGTGATT TTCTGGATGG CCTTGGCCCT
    GCCCAGCTAG TGGGGCGCCA AACTCTGGCC ACACCCGCCA TGGGTGACAT TCAGGTAGGA
    ATGATGGACA AAAAGGGACA GCTGGAGGTG GAAATAATTC GGGCCCGTGG CCTTGTTGTA
    AAACCAGGTT CCAAGACACT GCCAGCACCA TACGTAAAGG TGTATCTATT AGATAATGGA
    GTCTGCATAG CCAAAAAGAA AACAAAAGTG GCAAGAAAAA CGCTGGAACC CCTTTACCAG
    CAACTATTAT CTTTTGAAGA AAGTCCCCAA GGAAAGGTTT TACAGATCAT TGTCTGGGGA
    GACTATGGCC GCATGGATCA CAAATCCTTT ATGGGAGTGG CCCAGATACT TTTAGATGAA
    CTGGAGCTAT CCAACATGGT GATCGGCTGG TTCAAACTCT TCCCCCCTTC CTCCCTAGTA
    GATCCAACCT TGGCCCCTCT GACAAGAAGA GCTTCCCAAT CATCTCTGGA AAGTTCAACT
    GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857424.1
    CDS373..5436
    Translation

    Target ORF information:

    RefSeq Version XM_025001656.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAACA ATGGAGTCAA GGAGATAGAA CCTTATGAAG GTGCTATGGA TATAGAGGAG
    AGAAATCGCC AAATGAAAAT TAACAAATAC AAACAGGTAG CCGGATCAGA TCCCAGACTG
    GAACAAGATT ACCATGCAAA GTATCGATCG GGATGGGATC CTCATAGAGG GGCAGATAAT
    ATTTCCACTA AATCTTCGGA CAGTGACGTA AGTGATATAT CTGCGGTTTC CAGGACTAGT
    AGTGCTTCTC GTTTCAGCAG CACAAGCTAC ATGTCTGTCC AATCAGAACG GCCAAGAGGA
    AACAAGAAAA TCAGGCCAAA GGGACTAGAA GAGGGAGGGA AAGACGGGGA TAAGCATGAA
    GAGATAGTTC ATGCGGAGGA AGAGGTAAAG GAAGAAAGAA CTAATGAGAA TGAGAAAGGG
    AAAAACATTA CAAACATGTG TAATAAGGAG ACAAACAGAG AATCAGGAGA TGAGGAAAAA
    ACACCAGATA TACCTGAGCA AGGGAAAGAA AAAGAACAGT GGAATAAAGA GGATTTGCAA
    AAGCAATTCT CACAAGATGA TGACAGTGTC TTTACATCCA AAATGCAAAG CAGACAAATG
    GGCATCTCAG GGAAGAACAT GACCAAGAGC ACCAGCATCA GCGGAGACAT GTGCTCGCTG
    GAGAAGAATG ATGGCAGCCA GTCGGACACC GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG
    AAGCGACGCT CTAGCATTGG GGCCAAAATG GTAGCTATCG TTGGTCTTTC CCGGAAGAGC
    CGCAGTGCTT CTCAGCTCAG CCAAACGGAA ACCGGAGGTA AAAAGCTACG GAGCACTGTC
    CAGAGAAGTA CAGAAACAGG CTTGGCTGTG GAAATGAGGA ACTGGATGAC TCGACAAGCG
    AGCCGAGAAT CTACCGACGG CAGCATGAAC AGCTACAGCT CAGAAGGAAA TCTGATCTTC
    CCTGGTGTCC GCTTGGCCTC GGATAGCCAA TTCAGTGATT TTCTGGATGG CCTTGGCCCT
    GCCCAGCTAG TGGGGCGCCA AACTCTGGCC ACACCCGCCA TGGGTGACAT TCAGGTAGGA
    ATGATGGACA AAAAGGGACA GCTGGAGGTG GAAATAATTC GGGCCCGTGG CCTTGTTGTA
    AAACCAGGTT CCAAGACACT GCCAGCACCA TACGTAAAGG TGTATCTATT AGATAATGGA
    GTCTGCATAG CCAAAAAGAA AACAAAAGTG GCAAGAAAAA CGCTGGAACC CCTTTACCAG
    CAACTATTAT CTTTTGAAGA AAGTCCCCAA GGAAAGGTTT TACAGATCAT TGTCTGGGGA
    GACTATGGCC GCATGGATCA CAAATCCTTT ATGGGAGTGG CCCAGATACT TTTAGATGAA
    CTGGAGCTAT CCAACATGGT GATCGGCTGG TTCAAACTCT TCCCCCCTTC CTCCCTAGTA
    GATCCAACCT TGGCCCCTCT GACAAGAAGA GCTTCCCAAT CATCTCTGGA AAGTTCAACT
    GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222364
    Clone ID Related Accession (Same CDS sequence) XM_025001657.1
    Accession Version XM_025001657.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAACAAT
    GGAGTCAAGG AGATAGAACC TTATGAAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AAACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857425.1
    CDS373..5304
    Translation

    Target ORF information:

    RefSeq Version XM_025001657.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001657.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAAGC
    CAAAGCCTTA GTAGAAGAAC AACGCCTTTT GTTCCTAGGG TTCAGATAAA ACTGTGGTTT
    GACAAGGTTG GTCACCAATT AATAGTTACA ATTTTGGGAG CAAAAGACCT CCCTTCCAGG
    GAAGATGGGA GGCCAAGGAA TCCTTATGTT AAAATTTACT TTCTTCCAGA CAGAAGTGAC
    AAAAACAAGA GAAGAACTAA AACAGTGAAG AAAACTTTGG AACCCAAATG GAACCAAACA
    TTCATTTATT CTCCAGTCCA CCGAAGAGAG TTTCGGGAAC GGATGCTGGA GATTACCCTT
    TGGGATCAAG CTCGAGTTCG AGAGGAAGAA AGTGAATTTT TAGGAGAGAT TTTAATTGAA
    TTAGAAACAG CATTATTAGA TGATGAGCCA CATTGGTACA AACTTCAGAC TCACGATGTT
    TCTTCATTGC CACTTCCCCA TCCTTCACCA TATATGCCAC GACGACAACT CCATGGAGAG
    AGCCCAACAC GGAGGTTGCA AAGATCAAAG AGAATAAGTG ATAGTGAGGT CTCTGATTAT
    GACTGTGATG ATGGAATTGG TGTAGTATCA GATTATCGAC ACAATGGTCG GGATCTTCAA
    AGCTCAACAT TGTCAGTGCC AGAACAAGTA ATGTCATCCA ACCACTGTTC GCCGTCAGGG
    TCTCCACACC GAGTAGATGT TATAGGAAGG ACTAGATCTT GGTCACCCAG TGTCCCTCCT
    CCACAGAGTC GAAATGTGGA GCAGGGACTT CGAGGGACAC GCTCTGCTAT AGGACATTAT
    AATACAATTA GCCGAATGGA TAGACACCGT GTCATGGATG ACCATTATTC TCCAGACAGA
    GACAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAACAAT
    GGAGTCAAGG AGATAGAACC TTATGAAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AAACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222365
    Clone ID Related Accession (Same CDS sequence) XM_025001658.1
    Accession Version XM_025001658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAGTG CTATGGATAT AGAGGAGAGA AATCGCCAAA TGAAAATTAA CAAATACAAA
    CAGGTAGCCG GATCAGATCC CAGACTGGAA CAAGATTACC ATGCAAAGTA TCGATCGGGA
    TGGGATCCTC ATAGAGGGGC AGATAATATT TCCACTAAAT CTTCGGACAG TGACGTAAGT
    GATATATCTG CGGTTTCCAG GACTAGTAGT GCTTCTCGTT TCAGCAGCAC AAGCTACATG
    TCTGTCCAAT CAGAACGGCC AAGAGGAAAC AAGAAAATCA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857426.1
    CDS373..5151
    Translation

    Target ORF information:

    RefSeq Version XM_025001658.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAGTG CTATGGATAT AGAGGAGAGA AATCGCCAAA TGAAAATTAA CAAATACAAA
    CAGGTAGCCG GATCAGATCC CAGACTGGAA CAAGATTACC ATGCAAAGTA TCGATCGGGA
    TGGGATCCTC ATAGAGGGGC AGATAATATT TCCACTAAAT CTTCGGACAG TGACGTAAGT
    GATATATCTG CGGTTTCCAG GACTAGTAGT GCTTCTCGTT TCAGCAGCAC AAGCTACATG
    TCTGTCCAAT CAGAACGGCC AAGAGGAAAC AAGAAAATCA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92546
    Clone ID Related Accession (Same CDS sequence) XM_015474491.1
    Accession Version XM_015474491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAGAC
    GTGGACGCCA CAAGGAGAAG ACATGCAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AGACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329977.1
    CDS104..4975
    Translation

    Target ORF information:

    RefSeq Version XM_015474491.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAAAGAC
    GTGGACGCCA CAAGGAGAAG ACATGCAGGT GCTATGGATA TAGAGGAGAG AAATCGCCAA
    ATGAAAATTA ACAAATACAA ACAGGTAGCC GGATCAGATC CCAGACTGGA ACAAGATTAC
    CATGCAAAGT ATCGATCGGG ATGGGATCCT CATAGAGGGG CAGATAATAT TTCCACTAAA
    TCTTCGGACA GTGACGTAAG TGATATATCT GCGGTTTCCA GGACTAGTAG TGCTTCTCGT
    TTCAGCAGCA CAAGCTACAT GTCTGTCCAA TCAGAACGGC CAAGAGGAAA CAAGAAAATC
    AGGCCAAAGG GACTAGAAGA GGGAGGGAAA GACGGGGATA AGCATGAAGA GATAGTTCAT
    GCGGAGGAAG AGGTAAAGGA AGAAAGAACT AATGAGAATG AGAAAGGGAA AGACATTACA
    AACATGTGTA ATAAGGAGAC AAACAGAGAA TCAGGAGATG AGGAAAAAAC ACCAGATATA
    CCTGAGCAAG GGAAAGAAAA AGAACAGTGG AATAAAGAGG ATTTGCAAAA GCAATTCTCA
    CAAGATGATG ACAGTGTCTT TACATCCAAA ATGCAAAGCA GACAAATGGG CATCTCAGGG
    AAGAACATGA CCAAGAGCAC CAGCATCAGC GGAGACATGT GCTCGCTGGA GAAGAATGAT
    GGCAGCCAGT CGGACACCGC AGTGGGTGCC TTGGGCACCA GCAGCAAGAA GCGACGCTCT
    AGCATTGGGG CCAAAATGGT AGCTATCGTT GGTCTTTCCC GGAAGAGCCG CAGTGCTTCT
    CAGCTCAGCC AAACGGAAAC CGGAGGTAAA AAGCTACGGA GCACTGTCCA GAGAAGTACA
    GAAACAGGCT TGGCTGTGGA AATGAGGAAC TGGATGACTC GACAAGCGAG CCGAGAATCT
    ACCGACGGCA GCATGAACAG CTACAGCTCA GAAGGAAATC TGATCTTCCC TGGTGTCCGC
    TTGGCCTCGG ATAGCCAATT CAGTGATTTT CTGGATGGCC TTGGCCCTGC CCAGCTAGTG
    GGGCGCCAAA CTCTGGCCAC ACCCGCCATG GGTGACATTC AGGTAGGAAT GATGGACAAA
    AAGGGACAGC TGGAGGTGGA AATAATTCGG GCCCGTGGCC TTGTTGTAAA ACCAGGTTCC
    AAGACACTGC CAGCACCATA CGTAAAGGTG TATCTATTAG ATAATGGAGT CTGCATAGCC
    AAAAAGAAAA CAAAAGTGGC AAGAAAAACG CTGGAACCCC TTTACCAGCA ACTATTATCT
    TTTGAAGAAA GTCCCCAAGG AAAGGTTTTA CAGATCATTG TCTGGGGAGA CTATGGCCGC
    ATGGATCACA AATCCTTTAT GGGAGTGGCC CAGATACTTT TAGATGAACT GGAGCTATCC
    AACATGGTGA TCGGCTGGTT CAAACTCTTC CCCCCTTCCT CCCTAGTAGA TCCAACCTTG
    GCCCCTCTGA CAAGAAGAGC TTCCCAATCA TCTCTGGAAA GTTCAACTGG ACCTTCTTAC
    TCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92547
    Clone ID Related Accession (Same CDS sequence) XM_015474492.1
    Accession Version XM_015474492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAGTGCT
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGG CCAAAGGGAC TAGAAGAGGG AGGGAAAGAC
    GGGGATAAGC ATGAAGAGAT AGTTCATGCG GAGGAAGAGG TAAAGGAAGA AAGAACTAAT
    GAGAATGAGA AAGGGAAAGA CATTACAAAC ATGTGTAATA AGGAGACAAA CAGAGAATCA
    GGAGATGAGG AAAAAACACC AGATATACCT GAGCAAGGGA AAGAAAAAGA ACAGTGGAAT
    AAAGAGGATT TGCAAAAGCA ATTCTCACAA GATGATGACA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329978.1
    CDS104..4942
    Translation

    Target ORF information:

    RefSeq Version XM_015474492.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAGTGCT
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGG CCAAAGGGAC TAGAAGAGGG AGGGAAAGAC
    GGGGATAAGC ATGAAGAGAT AGTTCATGCG GAGGAAGAGG TAAAGGAAGA AAGAACTAAT
    GAGAATGAGA AAGGGAAAGA CATTACAAAC ATGTGTAATA AGGAGACAAA CAGAGAATCA
    GGAGATGAGG AAAAAACACC AGATATACCT GAGCAAGGGA AAGAAAAAGA ACAGTGGAAT
    AAAGAGGATT TGCAAAAGCA ATTCTCACAA GATGATGACA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92548
    Clone ID Related Accession (Same CDS sequence) XM_015474493.1
    Accession Version XM_015474493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA
    GACGTGGATC ACAACTGGTT GGACCATACG TCTTGGCATA GCAGTGAGGC ATCCCCAATG
    TCTTTGCACC CTGTGACGTG GCAGCCATCT AAAGATGGAG ATCGTTTAAT TGGTCGCATT
    TTATTAAATA AGCGTTTAAA AGATGGAAGT GTACCTCGAG ATTCAGGAGC AATGCTTGGC
    TTGAAGGTTG TAGGAGGAAA GATGACTGAA TCAGGTCGGC TCTGTGCATT TATAACCAAA
    GTTAAAAGAG GAAGTTTAGC TGATACTGTA GGACATCTTA GACCAGGTGA TGAAGTACTA
    GAGTGGAATG GAAGACTATT ACAAGGAGCC ACATTTGAAG AAGTATATAA CATCATCCTG
    GAATCCAAAC CTGAACCACA GGTGGAGCTT GTTGTTTCAA GGCCTATTGG AGATATACCT
    AGAATACCTG ACAGCACACA CACACAGCTG GAGTCCAGTT CTAGCTCATT CGAATCTCAA
    AAAATGGATC GTCCTTCTAT TTCTGTTACA TCTCCCATGA GTCCTGGCAT GTTGAGGGAT
    GTTCCACAGT TCTTATCTGG ACAACTTTCA AGCCAAAGCC TTAGTAGAAG AACAACGCCT
    TTTGTTCCTA GGGTTCAGAT AAAACTGTGG TTTGACAAGG TTGGTCACCA ATTAATAGTT
    ACAATTTTGG GAGCAAAAGA CCTCCCTTCC AGGGAAGATG GGAGGCCAAG GAATCCTTAT
    GTTAAAATTT ACTTTCTTCC AGACAGAAGT GACAAAAACA AGAGAAGAAC TAAAACAGTG
    AAGAAAACTT TGGAACCCAA ATGGAACCAA ACATTCATTT ATTCTCCAGT CCACCGAAGA
    GAGTTTCGGG AACGGATGCT GGAGATTACC CTTTGGGATC AAGCTCGAGT TCGAGAGGAA
    GAAAGTGAAT TTTTAGGAGA GATTTTAATT GAATTAGAAA CAGCATTATT AGATGATGAG
    CCACATTGGT ACAAACTTCA GACTCACGAT GTTTCTTCAT TGCCACTTCC CCATCCTTCA
    CCATATATGC CACGACGACA ACTCCATGGA GAGAGCCCAA CACGGAGGTT GCAAAGATCA
    AAGAGAATAA GTGATAGTGA GGTCTCTGAT TATGACTGTG ATGATGGAAT TGGTGTAGTA
    TCAGATTATC GACACAATGG TCGGGATCTT CAAAGCTCAA CATTGTCAGT GCCAGAACAA
    GTAATGTCAT CCAACCACTG TTCGCCGTCA GGGTCTCCAC ACCGAGTAGA TGTTATAGGA
    AGGACTAGAT CTTGGTCACC CAGTGTCCCT CCTCCACAGA GTCGAAATGT GGAGCAGGGA
    CTTCGAGGGA CACGCTCTGC TATAGGACAT TATAATACAA TTAGCCGAAT GGATAGACAC
    CGTGTCATGG ATGACCATTA TTCTCCAGAC AGAGACAGTC ATTTTCTTAC TCTACCTCGC
    TCCAGATACA GTCAGAGCGT TGAACATCAT CACAGGGATG GCAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAACAATG GAGTCAAGGA GATAGAACCT
    TATGAAGAAG ACGTGGACGC CACAAGGAGA AGACATGCAG GTGCTATGGA TATAGAGGAG
    AGAAATCGCC AAATGAAAAT TAACAAATAC AAACAGGTAG CCGGATCAGA TCCCAGACTG
    GAACAAGATT ACCATGCAAA GTATCGATCG GGATGGGATC CTCATAGAGG GGCAGATAAT
    ATTTCCACTA AATCTTCGGA CAGTGACGTA AGTGATATAT CTGCGGTTTC CAGGACTAGT
    AGTGCTTCTC GTTTCAGCAG CACAAGCTAC ATGTCTGTCC AATCAGAACG GCCAAGAGGA
    AACAAGAAAA TCAGGCCAAA GGGACTAGAA GAGGGAGGGA AAGACGGGGA TAAGCATGAA
    GAGATAGTTC ATGCGGAGGA AGAGGTAAAG GAAGAAAGAA CTAATGAGAA TGAGAAAGGG
    AAAGACATTA CAAACATGTG TAATAAGGAG ACAAACAGAG AATCAGGAGA TGAGGAAAAA
    ACACCAGATA TACCTGAGCA AGGGAAAGAA AAAGAACAGT GGAATAAAGA GGATTTGCAA
    AAGCAATTCT CACAAGATGA TGACAGTGTC TTTACATCCA AAATGCAAAG CAGACAAATG
    GGCATCTCAG GGAAGAACAT GACCAAGAGC ACCAGCATCA GCGGAGACAT GTGCTCGCTG
    GAGAAGAATG ATGGCAGCCA GTCGGACACC GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG
    AAGCGACGCT CTAGCATTGG GGCCAAAATG GTAGCTATCG TTGGTCTTTC CCGGAAGAGC
    CGCAGTGCTT CTCAGCTCAG CCAAACGGAA ACCGGAGGTA AAAAGCTACG GAGCACTGTC
    CAGAGAAGTA CAGAAACAGG CTTGGCTGTG GAAATGAGGA ACTGGATGAC TCGACAAGCG
    AGCCGAGAAT CTACCGACGG CAGCATGAAC AGCTACAGCT CAGAAGGAAA TCTGATCTTC
    CCTGGTGTCC GCTTGGCCTC GGATAGCCAA TTCAGTGATT TTCTGGATGG CCTTGGCCCT
    GCCCAGCTAG TGGGGCGCCA AACTCTGGCC ACACCCGCCA TGGGTGACAT TCAGGTAGGA
    ATGATGGACA AAAAGGGACA GCTGGAGGTG GAAATAATTC GGGCCCGTGG CCTTGTTGTA
    AAACCAGGTT CCAAGACACT GCCAGCACCA TACGTAAAGG TGTATCTATT AGATAATGGA
    GTCTGCATAG CCAAAAAGAA AACAAAAGTG GCAAGAAAAA CGCTGGAACC CCTTTACCAG
    CAACTATTAT CTTTTGAAGA AAGTCCCCAA GGAAAGGTTT TACAGATCAT TGTCTGGGGA
    GACTATGGCC GCATGGATCA CAAATCCTTT ATGGGAGTGG CCCAGATACT TTTAGATGAA
    CTGGAGCTAT CCAACATGGT GATCGGCTGG TTCAAACTCT TCCCCCCTTC CTCCCTAGTA
    GATCCAACCT TGGCCCCTCT GACAAGAAGA GCTTCCCAAT CATCTCTGGA AAGTTCAACT
    GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329979.1
    CDS104..4867
    Translation

    Target ORF information:

    RefSeq Version XM_015474493.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGA
    GACGTGGATC ACAACTGGTT GGACCATACG TCTTGGCATA GCAGTGAGGC ATCCCCAATG
    TCTTTGCACC CTGTGACGTG GCAGCCATCT AAAGATGGAG ATCGTTTAAT TGGTCGCATT
    TTATTAAATA AGCGTTTAAA AGATGGAAGT GTACCTCGAG ATTCAGGAGC AATGCTTGGC
    TTGAAGGTTG TAGGAGGAAA GATGACTGAA TCAGGTCGGC TCTGTGCATT TATAACCAAA
    GTTAAAAGAG GAAGTTTAGC TGATACTGTA GGACATCTTA GACCAGGTGA TGAAGTACTA
    GAGTGGAATG GAAGACTATT ACAAGGAGCC ACATTTGAAG AAGTATATAA CATCATCCTG
    GAATCCAAAC CTGAACCACA GGTGGAGCTT GTTGTTTCAA GGCCTATTGG AGATATACCT
    AGAATACCTG ACAGCACACA CACACAGCTG GAGTCCAGTT CTAGCTCATT CGAATCTCAA
    AAAATGGATC GTCCTTCTAT TTCTGTTACA TCTCCCATGA GTCCTGGCAT GTTGAGGGAT
    GTTCCACAGT TCTTATCTGG ACAACTTTCA AGCCAAAGCC TTAGTAGAAG AACAACGCCT
    TTTGTTCCTA GGGTTCAGAT AAAACTGTGG TTTGACAAGG TTGGTCACCA ATTAATAGTT
    ACAATTTTGG GAGCAAAAGA CCTCCCTTCC AGGGAAGATG GGAGGCCAAG GAATCCTTAT
    GTTAAAATTT ACTTTCTTCC AGACAGAAGT GACAAAAACA AGAGAAGAAC TAAAACAGTG
    AAGAAAACTT TGGAACCCAA ATGGAACCAA ACATTCATTT ATTCTCCAGT CCACCGAAGA
    GAGTTTCGGG AACGGATGCT GGAGATTACC CTTTGGGATC AAGCTCGAGT TCGAGAGGAA
    GAAAGTGAAT TTTTAGGAGA GATTTTAATT GAATTAGAAA CAGCATTATT AGATGATGAG
    CCACATTGGT ACAAACTTCA GACTCACGAT GTTTCTTCAT TGCCACTTCC CCATCCTTCA
    CCATATATGC CACGACGACA ACTCCATGGA GAGAGCCCAA CACGGAGGTT GCAAAGATCA
    AAGAGAATAA GTGATAGTGA GGTCTCTGAT TATGACTGTG ATGATGGAAT TGGTGTAGTA
    TCAGATTATC GACACAATGG TCGGGATCTT CAAAGCTCAA CATTGTCAGT GCCAGAACAA
    GTAATGTCAT CCAACCACTG TTCGCCGTCA GGGTCTCCAC ACCGAGTAGA TGTTATAGGA
    AGGACTAGAT CTTGGTCACC CAGTGTCCCT CCTCCACAGA GTCGAAATGT GGAGCAGGGA
    CTTCGAGGGA CACGCTCTGC TATAGGACAT TATAATACAA TTAGCCGAAT GGATAGACAC
    CGTGTCATGG ATGACCATTA TTCTCCAGAC AGAGACAGTC ATTTTCTTAC TCTACCTCGC
    TCCAGATACA GTCAGAGCGT TGAACATCAT CACAGGGATG GCAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAACAATG GAGTCAAGGA GATAGAACCT
    TATGAAGAAG ACGTGGACGC CACAAGGAGA AGACATGCAG GTGCTATGGA TATAGAGGAG
    AGAAATCGCC AAATGAAAAT TAACAAATAC AAACAGGTAG CCGGATCAGA TCCCAGACTG
    GAACAAGATT ACCATGCAAA GTATCGATCG GGATGGGATC CTCATAGAGG GGCAGATAAT
    ATTTCCACTA AATCTTCGGA CAGTGACGTA AGTGATATAT CTGCGGTTTC CAGGACTAGT
    AGTGCTTCTC GTTTCAGCAG CACAAGCTAC ATGTCTGTCC AATCAGAACG GCCAAGAGGA
    AACAAGAAAA TCAGGCCAAA GGGACTAGAA GAGGGAGGGA AAGACGGGGA TAAGCATGAA
    GAGATAGTTC ATGCGGAGGA AGAGGTAAAG GAAGAAAGAA CTAATGAGAA TGAGAAAGGG
    AAAGACATTA CAAACATGTG TAATAAGGAG ACAAACAGAG AATCAGGAGA TGAGGAAAAA
    ACACCAGATA TACCTGAGCA AGGGAAAGAA AAAGAACAGT GGAATAAAGA GGATTTGCAA
    AAGCAATTCT CACAAGATGA TGACAGTGTC TTTACATCCA AAATGCAAAG CAGACAAATG
    GGCATCTCAG GGAAGAACAT GACCAAGAGC ACCAGCATCA GCGGAGACAT GTGCTCGCTG
    GAGAAGAATG ATGGCAGCCA GTCGGACACC GCAGTGGGTG CCTTGGGCAC CAGCAGCAAG
    AAGCGACGCT CTAGCATTGG GGCCAAAATG GTAGCTATCG TTGGTCTTTC CCGGAAGAGC
    CGCAGTGCTT CTCAGCTCAG CCAAACGGAA ACCGGAGGTA AAAAGCTACG GAGCACTGTC
    CAGAGAAGTA CAGAAACAGG CTTGGCTGTG GAAATGAGGA ACTGGATGAC TCGACAAGCG
    AGCCGAGAAT CTACCGACGG CAGCATGAAC AGCTACAGCT CAGAAGGAAA TCTGATCTTC
    CCTGGTGTCC GCTTGGCCTC GGATAGCCAA TTCAGTGATT TTCTGGATGG CCTTGGCCCT
    GCCCAGCTAG TGGGGCGCCA AACTCTGGCC ACACCCGCCA TGGGTGACAT TCAGGTAGGA
    ATGATGGACA AAAAGGGACA GCTGGAGGTG GAAATAATTC GGGCCCGTGG CCTTGTTGTA
    AAACCAGGTT CCAAGACACT GCCAGCACCA TACGTAAAGG TGTATCTATT AGATAATGGA
    GTCTGCATAG CCAAAAAGAA AACAAAAGTG GCAAGAAAAA CGCTGGAACC CCTTTACCAG
    CAACTATTAT CTTTTGAAGA AAGTCCCCAA GGAAAGGTTT TACAGATCAT TGTCTGGGGA
    GACTATGGCC GCATGGATCA CAAATCCTTT ATGGGAGTGG CCCAGATACT TTTAGATGAA
    CTGGAGCTAT CCAACATGGT GATCGGCTGG TTCAAACTCT TCCCCCCTTC CTCCCTAGTA
    GATCCAACCT TGGCCCCTCT GACAAGAAGA GCTTCCCAAT CATCTCTGGA AAGTTCAACT
    GGACCTTCTT ACTCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92549
    Clone ID Related Accession (Same CDS sequence) XM_015474494.1
    Accession Version XM_015474494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAGTGCT
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGT GTCTTTACAT CCAAAATGCA AAGCAGACAA
    ATGGGCATCT CAGGGAAGAA CATGACCAAG AGCACCAGCA TCAGCGGAGA CATGTGCTCG
    CTGGAGAAGA ATGATGGCAG CCAGTCGGAC ACCGCAGTGG GTGCCTTGGG CACCAGCAGC
    AAGAAGCGAC GCTCTAGCAT TGGGGCCAAA ATGGTAGCTA TCGTTGGTCT TTCCCGGAAG
    AGCCGCAGTG CTTCTCAGCT CAGCCAAACG GAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329980.1
    CDS104..4690
    Translation

    Target ORF information:

    RefSeq Version XM_015474494.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTATAAGG AGCAGGTAAA GAAGATGGGT GAAGAATCAC AGCAGCAACA AGAACAGAAG 
    GGTGATGCTC CAACCTGTGG CATCTGCCAC AAAACCAAGT TTGCTGACGG ATGTGGCCAT
    AACTGTTCAT ATTGCCAAAC CAAGTTCTGT GCTCGCTGTG GAGGTCGCGT GTCGTTACGC
    TCAAACAAGG AGGACAAAGT GGTTATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACTA AATCCGGAGC ATGGTTTTAT AATAGTGGAT CTAATACACC ACAGCAACCT
    GATCAAAAGG TTCTCCGAGG ACTGCGGAAT GAGGAGGCGC CTCAGGAGAA GAAAGCAAAA
    CTGCATGAGC AGGCCCAGTT CCAAGGACCC TCAGGTGACT TATCTGTACC TGCAGTGGAG
    AAAAGTCGAT CTCATGGGCT CACAAGACAG GATTCTATTA AAAATGGGTC AGGAGTGAAG
    CACCAAATTG CCAGTGACGT AGCTTCAGAC AGGAAAAGAA GTCCATCAGT GTCCAGAGAT
    CAGAACAGAA GATACGACCA AAGGGAAGAA AGAGAAGAAT ATTCACAGTA TGCTACTTCG
    GACAGCGCAA TGCCTAGATC TCCATCAGAT TATGCTGATA GGAGGTCTCA ACCTGAACCT
    CAGTTTTATG AAGAGTCTGA TCATATAAAT TATAGGGACT CCAACAGGAG AAGTCATAGA
    CATTCCAAAG AATATATTGT AGATGACAAG GATGTGGAGA GTAGAGATGA ATATGAAAGA
    CAGAGGAGAG AGGAGGAATA CCAGGCGCGC TATCGAAGTG ATCCGAATTT GGCCCGTTAT
    CCAGTCAAGC CACAGCCCTA TGAAGAACAA ATGCGTATCC ATGCTGAAGT GTCACGAGCC
    AGGCATGAGA GAAGGCACAG TGATGTTTCT TTGGCAAATG CTGAATTGGA AGACTCCAGG
    ATTTCTATGC TAAGGATGGA ACGACCATCA AGGCAAAGGT CTATATCTGA ACGTAGAGCT
    GCCATGGAAA ATCAGCGATC TTATTCAATG GAAAGAACTC GAGAGGCTCA GGGACCAAGT
    TCTTATCCAC AAAGGACCAC AAACCATAGT CCTCCTACCC CCCGGAGGAG TCCAATACCC
    ATTGATAGAC CAGACGTGAG ACGTACTGAC TCACTAAGGA AACAACACCA CCTAGATCCT
    AGTTCTGCTG TAAGGAAAAC GAAACGGGAA AAAATGGAAA CAATGTTAAG GAATGATTCT
    CTCAGTTCTG ACCAGTCAGA GTCAGTGAGA CCTCCACCAC CAAAGCCTCA TAAATCAAAG
    AAAGGAGGTA AAATGCGCCA GGTTTCATTG AGCAGTTCAG AGGAGGAATT AGCTTCTACA
    CCTGAATATA CCAGTTGCGA TGATGTTGAG ATTGAAAGTG AGAGTGTGAG TGAAAAAGGG
    GACAGTCAAA AGGGAAAAAG AAAAACTAGT GAGCAGGCAG TTTTGTCGGA CTCTAACACC
    AGGTCTGAGA GACAAAAGAA AATGATGTAC TTTGGAGGCC ACTCTTTGGA AGAGGATTTG
    GAATGGTCTG AGCCTCAGAT TAAGGACTCT GGGGTAGATA CGTGTAGTAG CACAACCCTT
    AATGAGGAGC ATAGCCATAG TGATAAGCAC CCTGTGACGT GGCAGCCATC TAAAGATGGA
    GATCGTTTAA TTGGTCGCAT TTTATTAAAT AAGCGTTTAA AAGATGGAAG TGTACCTCGA
    GATTCAGGAG CAATGCTTGG CTTGAAGGTT GTAGGAGGAA AGATGACTGA ATCAGGTCGG
    CTCTGTGCAT TTATAACCAA AGTTAAAAGA GGAAGTTTAG CTGATACTGT AGGACATCTT
    AGACCAGGTG ATGAAGTACT AGAGTGGAAT GGAAGACTAT TACAAGGAGC CACATTTGAA
    GAAGTATATA ACATCATCCT GGAATCCAAA CCTGAACCAC AGGTGGAGCT TGTTGTTTCA
    AGGCCTATTG GAGATATACC TAGAATACCT GACAGCACAC ACACACAGCT GGAGTCCAGT
    TCTAGCTCAT TCGAATCTCA AAAAATGGAT CGTCCTTCTA TTTCTGTTAC ATCTCCCATG
    AGTCCTGGCA TGTTGAGGGA TGTTCCACAG TTCTTATCTG GACAACTTTC AAGCCAAAGC
    CTTAGTAGAA GAACAACGCC TTTTGTTCCT AGGGTTCAGA TAAAACTGTG GTTTGACAAG
    GTTGGTCACC AATTAATAGT TACAATTTTG GGAGCAAAAG ACCTCCCTTC CAGGGAAGAT
    GGGAGGCCAA GGAATCCTTA TGTTAAAATT TACTTTCTTC CAGACAGAAG TGACAAAAAC
    AAGAGAAGAA CTAAAACAGT GAAGAAAACT TTGGAACCCA AATGGAACCA AACATTCATT
    TATTCTCCAG TCCACCGAAG AGAGTTTCGG GAACGGATGC TGGAGATTAC CCTTTGGGAT
    CAAGCTCGAG TTCGAGAGGA AGAAAGTGAA TTTTTAGGAG AGATTTTAAT TGAATTAGAA
    ACAGCATTAT TAGATGATGA GCCACATTGG TACAAACTTC AGACTCACGA TGTTTCTTCA
    TTGCCACTTC CCCATCCTTC ACCATATATG CCACGACGAC AACTCCATGG AGAGAGCCCA
    ACACGGAGGT TGCAAAGATC AAAGAGAATA AGTGATAGTG AGGTCTCTGA TTATGACTGT
    GATGATGGAA TTGGTGTAGT ATCAGATTAT CGACACAATG GTCGGGATCT TCAAAGCTCA
    ACATTGTCAG TGCCAGAACA AGTAATGTCA TCCAACCACT GTTCGCCGTC AGGGTCTCCA
    CACCGAGTAG ATGTTATAGG AAGGACTAGA TCTTGGTCAC CCAGTGTCCC TCCTCCACAG
    AGTCGAAATG TGGAGCAGGG ACTTCGAGGG ACACGCTCTG CTATAGGACA TTATAATACA
    ATTAGCCGAA TGGATAGACA CCGTGTCATG GATGACCATT ATTCTCCAGA CAGAGACAGT
    CATTTTCTTA CTCTACCTCG CTCCAGATAC AGTCAGAGCG TTGAACATCA TCACAGGGAT
    GGCAGGGATT GTGAAGCAGC AGATAGACAG CCATATCACA GATCCAGATC AACAGAACAG
    CGGCCTCTCC TAGAGCGGAC CACCACCCGC TCCAGATCCA CTGAACGTCC TGATACAAAC
    CTCATGAGGT CGATGCCTTC ATTAATGACT GGAAGATCTG CCCCTCCTTC ACCTGCCTTA
    TCGAGGTCTC ATCCTCGTAC TGGGTCTGTC CAAACAAGCC CATCAAGTAC TCCAGTGGCA
    GGACGAAGGG GCCGACAACT CCCACAGCTT CCACCAAAGG GAACATTGGA GAGAAGTGCT
    ATGGATATAG AGGAGAGAAA TCGCCAAATG AAAATTAACA AATACAAACA GGTAGCCGGA
    TCAGATCCCA GACTGGAACA AGATTACCAT GCAAAGTATC GATCGGGATG GGATCCTCAT
    AGAGGGGCAG ATAATATTTC CACTAAATCT TCGGACAGTG ACGTAAGTGA TATATCTGCG
    GTTTCCAGGA CTAGTAGTGC TTCTCGTTTC AGCAGCACAA GCTACATGTC TGTCCAATCA
    GAACGGCCAA GAGGAAACAA GAAAATCAGT GTCTTTACAT CCAAAATGCA AAGCAGACAA
    ATGGGCATCT CAGGGAAGAA CATGACCAAG AGCACCAGCA TCAGCGGAGA CATGTGCTCG
    CTGGAGAAGA ATGATGGCAG CCAGTCGGAC ACCGCAGTGG GTGCCTTGGG CACCAGCAGC
    AAGAAGCGAC GCTCTAGCAT TGGGGCCAAA ATGGTAGCTA TCGTTGGTCT TTCCCGGAAG
    AGCCGCAGTG CTTCTCAGCT CAGCCAAACG GAAACCGGAG GTAAAAAGCT ACGGAGCACT
    GTCCAGAGAA GTACAGAAAC AGGCTTGGCT GTGGAAATGA GGAACTGGAT GACTCGACAA
    GCGAGCCGAG AATCTACCGA CGGCAGCATG AACAGCTACA GCTCAGAAGG AAATCTGATC
    TTCCCTGGTG TCCGCTTGGC CTCGGATAGC CAATTCAGTG ATTTTCTGGA TGGCCTTGGC
    CCTGCCCAGC TAGTGGGGCG CCAAACTCTG GCCACACCCG CCATGGGTGA CATTCAGGTA
    GGAATGATGG ACAAAAAGGG ACAGCTGGAG GTGGAAATAA TTCGGGCCCG TGGCCTTGTT
    GTAAAACCAG GTTCCAAGAC ACTGCCAGCA CCATACGTAA AGGTGTATCT ATTAGATAAT
    GGAGTCTGCA TAGCCAAAAA GAAAACAAAA GTGGCAAGAA AAACGCTGGA ACCCCTTTAC
    CAGCAACTAT TATCTTTTGA AGAAAGTCCC CAAGGAAAGG TTTTACAGAT CATTGTCTGG
    GGAGACTATG GCCGCATGGA TCACAAATCC TTTATGGGAG TGGCCCAGAT ACTTTTAGAT
    GAACTGGAGC TATCCAACAT GGTGATCGGC TGGTTCAAAC TCTTCCCCCC TTCCTCCCTA
    GTAGATCCAA CCTTGGCCCC TCTGACAAGA AGAGCTTCCC AATCATCTCT GGAAAGTTCA
    ACTGGACCTT CTTACTCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26045
    Clone ID Related Accession (Same CDS sequence) XM_005215573.3
    Accession Version XM_005215573.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104398.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAAGCC AAAGCCTTAG TAGAAGAACA
    ACGCCTTTTG TTCCTAGGGT TCAGATAAAA CTGTGGTTTG ACAAGGTTGG TCACCAATTA
    ATAGTTACAA TTTTGGGAGC AAAAGACCTC CCTTCCAGGG AAGATGGGAG GCCAAGGAAT
    CCTTATGTTA AAATTTACTT TCTTCCAGAC AGAAGTGACA AAAACAAGAG AAGAACTAAA
    ACAGTGAAGA AAACTTTGGA ACCCAAATGG AACCAAACAT TCATTTATTC TCCAGTCCAC
    CGAAGAGAGT TTCGGGAACG GATGCTGGAG ATTACCCTTT GGGATCAAGC TCGAGTTCGA
    GAGGAAGAAA GTGAATTTTT AGGAGAGATT TTAATTGAAT TAGAAACAGC ATTATTAGAT
    GATGAGCCAC ATTGGTACAA ACTTCAGACT CACGATGTTT CTTCATTGCC ACTTCCCCAT
    CCTTCACCAT ATATGCCACG ACGACAACTC CATGGAGAGA GCCCAACACG GAGGTTGCAA
    AGATCAAAGA GAATAAGTGA TAGTGAGGTC TCTGATTATG ACTGTGATGA TGGAATTGGT
    GTAGTATCAG ATTATCGACA CAATGGTCGG GATCTTCAAA GCTCAACATT GTCAGTGCCA
    GAACAAGTAA TGTCATCCAA CCACTGTTCG CCGTCAGGGT CTCCACACCG AGTAGATGTT
    ATAGGAAGGA CTAGATCTTG GTCACCCAGT GTCCCTCCTC CACAGAGTCG AAATGTGGAG
    CAGGGACTTC GAGGGACACG CTCTGCTATA GGACATTATA ATACAATTAG CCGAATGGAT
    AGACACCGTG TCATGGATGA CCATTATTCT CCAGACAGAG ACAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAAAACCG GAGGTAAAAA GCTACGGAGC
    ACTGTCCAGA GAAGTACAGA AACAGGCTTG GCTGTGGAAA TGAGGAACTG GATGACTCGA
    CAAGCGAGCC GAGAATCTAC CGACGGCAGC ATGAACAGCT ACAGCTCAGA AGGAAATCTG
    ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT AGCCAATTCA GTGATTTTCT GGATGGCCTT
    GGCCCTGCCC AGCTAGTGGG GCGCCAAACT CTGGCCACAC CCGCCATGGG TGACATTCAG
    GTAGGAATGA TGGACAAAAA GGGACAGCTG GAGGTGGAAA TAATTCGGGC CCGTGGCCTT
    GTTGTAAAAC CAGGTTCCAA GACACTGCCA GCACCATACG TAAAGGTGTA TCTATTAGAT
    AATGGAGTCT GCATAGCCAA AAAGAAAACA AAAGTGGCAA GAAAAACGCT GGAACCCCTT
    TACCAGCAAC TATTATCTTT TGAAGAAAGT CCCCAAGGAA AGGTTTTACA GATCATTGTC
    TGGGGAGACT ATGGCCGCAT GGATCACAAA TCCTTTATGG GAGTGGCCCA GATACTTTTA
    GATGAACTGG AGCTATCCAA CATGGTGATC GGCTGGTTCA AACTCTTCCC CCCTTCCTCC
    CTAGTAGATC CAACCTTGGC CCCTCTGACA AGAAGAGCTT CCCAATCATC TCTGGAAAGT
    TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215630.1
    CDS381..4430
    Translation

    Target ORF information:

    RefSeq Version XM_005215573.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215573.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGAGACG TGGATCACAA CTGGTTGGAC CATACGTCTT GGCATAGCAG TGAGGCATCC
    CCAATGTCTT TGCACCCTGT GACGTGGCAG CCATCTAAAG ATGGAGATCG TTTAATTGGT
    CGCATTTTAT TAAATAAGCG TTTAAAAGAT GGAAGTGTAC CTCGAGATTC AGGAGCAATG
    CTTGGCTTGA AGGTTGTAGG AGGAAAGATG ACTGAATCAG GTCGGCTCTG TGCATTTATA
    ACCAAAGTTA AAAGAGGAAG TTTAGCTGAT ACTGTAGGAC ATCTTAGACC AGGTGATGAA
    GTACTAGAGT GGAATGGAAG ACTATTACAA GGAGCCACAT TTGAAGAAGT ATATAACATC
    ATCCTGGAAT CCAAACCTGA ACCACAGGTG GAGCTTGTTG TTTCAAGGCC TATTGGAGAT
    ATACCTAGAA TACCTGACAG CACACACACA CAGCTGGAGT CCAGTTCTAG CTCATTCGAA
    TCTCAAAAAA TGGATCGTCC TTCTATTTCT GTTACATCTC CCATGAGTCC TGGCATGTTG
    AGGGATGTTC CACAGTTCTT ATCTGGACAA CTTTCAAGCC AAAGCCTTAG TAGAAGAACA
    ACGCCTTTTG TTCCTAGGGT TCAGATAAAA CTGTGGTTTG ACAAGGTTGG TCACCAATTA
    ATAGTTACAA TTTTGGGAGC AAAAGACCTC CCTTCCAGGG AAGATGGGAG GCCAAGGAAT
    CCTTATGTTA AAATTTACTT TCTTCCAGAC AGAAGTGACA AAAACAAGAG AAGAACTAAA
    ACAGTGAAGA AAACTTTGGA ACCCAAATGG AACCAAACAT TCATTTATTC TCCAGTCCAC
    CGAAGAGAGT TTCGGGAACG GATGCTGGAG ATTACCCTTT GGGATCAAGC TCGAGTTCGA
    GAGGAAGAAA GTGAATTTTT AGGAGAGATT TTAATTGAAT TAGAAACAGC ATTATTAGAT
    GATGAGCCAC ATTGGTACAA ACTTCAGACT CACGATGTTT CTTCATTGCC ACTTCCCCAT
    CCTTCACCAT ATATGCCACG ACGACAACTC CATGGAGAGA GCCCAACACG GAGGTTGCAA
    AGATCAAAGA GAATAAGTGA TAGTGAGGTC TCTGATTATG ACTGTGATGA TGGAATTGGT
    GTAGTATCAG ATTATCGACA CAATGGTCGG GATCTTCAAA GCTCAACATT GTCAGTGCCA
    GAACAAGTAA TGTCATCCAA CCACTGTTCG CCGTCAGGGT CTCCACACCG AGTAGATGTT
    ATAGGAAGGA CTAGATCTTG GTCACCCAGT GTCCCTCCTC CACAGAGTCG AAATGTGGAG
    CAGGGACTTC GAGGGACACG CTCTGCTATA GGACATTATA ATACAATTAG CCGAATGGAT
    AGACACCGTG TCATGGATGA CCATTATTCT CCAGACAGAG ACAGGGATTG TGAAGCAGCA
    GATAGACAGC CATATCACAG ATCCAGATCA ACAGAACAGC GGCCTCTCCT AGAGCGGACC
    ACCACCCGCT CCAGATCCAC TGAACGTCCT GATACAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACTG GAAGATCTGC CCCTCCTTCA CCTGCCTTAT CGAGGTCTCA TCCTCGTACT
    GGGTCTGTCC AAACAAGCCC ATCAAGTACT CCAGTGGCAG GACGAAGGGG CCGACAACTC
    CCACAGCTTC CACCAAAGGG AACATTGGAG AGAAAAACCG GAGGTAAAAA GCTACGGAGC
    ACTGTCCAGA GAAGTACAGA AACAGGCTTG GCTGTGGAAA TGAGGAACTG GATGACTCGA
    CAAGCGAGCC GAGAATCTAC CGACGGCAGC ATGAACAGCT ACAGCTCAGA AGGAAATCTG
    ATCTTCCCTG GTGTCCGCTT GGCCTCGGAT AGCCAATTCA GTGATTTTCT GGATGGCCTT
    GGCCCTGCCC AGCTAGTGGG GCGCCAAACT CTGGCCACAC CCGCCATGGG TGACATTCAG
    GTAGGAATGA TGGACAAAAA GGGACAGCTG GAGGTGGAAA TAATTCGGGC CCGTGGCCTT
    GTTGTAAAAC CAGGTTCCAA GACACTGCCA GCACCATACG TAAAGGTGTA TCTATTAGAT
    AATGGAGTCT GCATAGCCAA AAAGAAAACA AAAGTGGCAA GAAAAACGCT GGAACCCCTT
    TACCAGCAAC TATTATCTTT TGAAGAAAGT CCCCAAGGAA AGGTTTTACA GATCATTGTC
    TGGGGAGACT ATGGCCGCAT GGATCACAAA TCCTTTATGG GAGTGGCCCA GATACTTTTA
    GATGAACTGG AGCTATCCAA CATGGTGATC GGCTGGTTCA AACTCTTCCC CCCTTCCTCC
    CTAGTAGATC CAACCTTGGC CCCTCTGACA AGAAGAGCTT CCCAATCATC TCTGGAAAGT
    TCAACTGGAC CTTCTTACTC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53231
    Clone ID Related Accession (Same CDS sequence) XM_025001659.1
    Accession Version XM_025001659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAGTG CTATGGATAT AGAGGAGAGA AATCGCCAAA TGAAAATTAA CAAATACAAA
    CAGGTAGCCG GATCAGATCC CAGACTGGAA CAAGATTACC ATGCAAAGTA TCGATCGGGA
    TGGGATCCTC ATAGAGGGGC AGATAATATT TCCACTAAAT CTTCGGACAG TGACGTAAGT
    GATATATCTG CGGTTTCCAG GACTAGTAGT GCTTCTCGTT TCAGCAGCAC AAGCTACATG
    TCTGTCCAAT CAGAACGGCC AAGAGGAAAC AAGAAAATCA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857427.1
    CDS1..4599
    Translation

    Target ORF information:

    RefSeq Version XM_025001659.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAGTG CTATGGATAT AGAGGAGAGA AATCGCCAAA TGAAAATTAA CAAATACAAA
    CAGGTAGCCG GATCAGATCC CAGACTGGAA CAAGATTACC ATGCAAAGTA TCGATCGGGA
    TGGGATCCTC ATAGAGGGGC AGATAATATT TCCACTAAAT CTTCGGACAG TGACGTAAGT
    GATATATCTG CGGTTTCCAG GACTAGTAGT GCTTCTCGTT TCAGCAGCAC AAGCTACATG
    TCTGTCCAAT CAGAACGGCC AAGAGGAAAC AAGAAAATCA GTGTCTTTAC ATCCAAAATG
    CAAAGCAGAC AAATGGGCAT CTCAGGGAAG AACATGACCA AGAGCACCAG CATCAGCGGA
    GACATGTGCT CGCTGGAGAA GAATGATGGC AGCCAGTCGG ACACCGCAGT GGGTGCCTTG
    GGCACCAGCA GCAAGAAGCG ACGCTCTAGC ATTGGGGCCA AAATGGTAGC TATCGTTGGT
    CTTTCCCGGA AGAGCCGCAG TGCTTCTCAG CTCAGCCAAA CGGAAACCGG AGGTAAAAAG
    CTACGGAGCA CTGTCCAGAG AAGTACAGAA ACAGGCTTGG CTGTGGAAAT GAGGAACTGG
    ATGACTCGAC AAGCGAGCCG AGAATCTACC GACGGCAGCA TGAACAGCTA CAGCTCAGAA
    GGAAATCTGA TCTTCCCTGG TGTCCGCTTG GCCTCGGATA GCCAATTCAG TGATTTTCTG
    GATGGCCTTG GCCCTGCCCA GCTAGTGGGG CGCCAAACTC TGGCCACACC CGCCATGGGT
    GACATTCAGG TAGGAATGAT GGACAAAAAG GGACAGCTGG AGGTGGAAAT AATTCGGGCC
    CGTGGCCTTG TTGTAAAACC AGGTTCCAAG ACACTGCCAG CACCATACGT AAAGGTGTAT
    CTATTAGATA ATGGAGTCTG CATAGCCAAA AAGAAAACAA AAGTGGCAAG AAAAACGCTG
    GAACCCCTTT ACCAGCAACT ATTATCTTTT GAAGAAAGTC CCCAAGGAAA GGTTTTACAG
    ATCATTGTCT GGGGAGACTA TGGCCGCATG GATCACAAAT CCTTTATGGG AGTGGCCCAG
    ATACTTTTAG ATGAACTGGA GCTATCCAAC ATGGTGATCG GCTGGTTCAA ACTCTTCCCC
    CCTTCCTCCC TAGTAGATCC AACCTTGGCC CCTCTGACAA GAAGAGCTTC CCAATCATCT
    CTGGAAAGTT CAACTGGACC TTCTTACTCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53232
    Clone ID Related Accession (Same CDS sequence) XM_025001661.1
    Accession Version XM_025001661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAAAA CCGGAGGTAA AAAGCTACGG AGCACTGTCC AGAGAAGTAC AGAAACAGGC
    TTGGCTGTGG AAATGAGGAA CTGGATGACT CGACAAGCGA GCCGAGAATC TACCGACGGC
    AGCATGAACA GCTACAGCTC AGAAGGAAAT CTGATCTTCC CTGGTGTCCG CTTGGCCTCG
    GATAGCCAAT TCAGTGATTT TCTGGATGGC CTTGGCCCTG CCCAGCTAGT GGGGCGCCAA
    ACTCTGGCCA CACCCGCCAT GGGTGACATT CAGGTAGGAA TGATGGACAA AAAGGGACAG
    CTGGAGGTGG AAATAATTCG GGCCCGTGGC CTTGTTGTAA AACCAGGTTC CAAGACACTG
    CCAGCACCAT ACGTAAAGGT GTATCTATTA GATAATGGAG TCTGCATAGC CAAAAAGAAA
    ACAAAAGTGG CAAGAAAAAC GCTGGAACCC CTTTACCAGC AACTATTATC TTTTGAAGAA
    AGTCCCCAAG GAAAGGTTTT ACAGATCATT GTCTGGGGAG ACTATGGCCG CATGGATCAC
    AAATCCTTTA TGGGAGTGGC CCAGATACTT TTAGATGAAC TGGAGCTATC CAACATGGTG
    ATCGGCTGGT TCAAACTCTT CCCCCCTTCC TCCCTAGTAG ATCCAACCTT GGCCCCTCTG
    ACAAGAAGAG CTTCCCAATC ATCTCTGGAA AGTTCAACTG GACCTTCTTA CTCTCGTTCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857429.1
    CDS373..4635
    Translation

    Target ORF information:

    RefSeq Version XM_025001661.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCGGCTC CTGTCGGGCC GCGGGGCCGC CCGGCTCCCA CCCCGGCGGG CTCTCAGCCG 
    CCTCTGCAGC CCGAGATGCC CGACCTCAGC CACCTCACGG AGGAGGAGAG GAAAATCATC
    CTGGCCGTCA TGGATCGGCA GAAGAAAGAA GAGGAGAAAG AGCAGTCGGT GCTGAAGGTC
    AAAGAAGAAC ACAAACCACA GACGACACAG TGGTTTCCCT TTAGTGGGAT CACTGAACTG
    GTAAATAACG TTCTTCAGCC CCAGCAAAAA CAACAAAATG AAAAGGAGCC CCAGACAAAA
    CTTCATCAGC AGTTTGAAAT GTATAAGGAG CAGGTAAAGA AGATGGGTGA AGAATCACAG
    CAGCAACAAG AACAGAAGGG TGATGCTCCA ACCTGTGGCA TCTGCCACAA AACCAAGTTT
    GCTGACGGAT GTGGCCATAA CTGTTCATAT TGCCAAACCA AGTTCTGTGC TCGCTGTGGA
    GGTCGCGTGT CGTTACGCTC AAACAAGGTT ATGTGGGTAT GTAATTTGTG CCGAAAACAA
    CAAGAAATCC TCACTAAATC CGGAGCATGG TTTTATAATA GTGGATCTAA TACACCACAG
    CAACCTGATC AAAAGGTTCT CCGAGGACTG CGGAATGAGG AGGCGCCTCA GGAGAAGAAA
    GCAAAACTGC ATGAGCAGGC CCAGTTCCAA GGACCCTCAG GTGACTTATC TGTACCTGCA
    GTGGAGAAAA GTCGATCTCA TGGGCTCACA AGACAGGATT CTATTAAAAA TGGGTCAGGA
    GTGAAGCACC AAATTGCCAG TGACGTAGCT TCAGACAGGA AAAGAAGTCC ATCAGTGTCC
    AGAGATCAGA ACAGAAGATA CGACCAAAGG GAAGAAAGAG AAGAATATTC ACAGTATGCT
    ACTTCGGACA GCGCAATGCC TAGATCTCCA TCAGATTATG CTGATAGGAG GTCTCAACCT
    GAACCTCAGT TTTATGAAGA GTCTGATCAT ATAAATTATA GGGACTCCAA CAGGAGAAGT
    CATAGACATT CCAAAGAATA TATTGTAGAT GACAAGGATG TGGAGAGTAG AGATGAATAT
    GAAAGACAGA GGAGAGAGGA GGAATACCAG GCGCGCTATC GAAGTGATCC GAATTTGGCC
    CGTTATCCAG TCAAGCCACA GCCCTATGAA GAACAAATGC GTATCCATGC TGAAGTGTCA
    CGAGCCAGGC ATGAGAGAAG GCACAGTGAT GTTTCTTTGG CAAATGCTGA ATTGGAAGAC
    TCCAGGATTT CTATGCTAAG GATGGAACGA CCATCAAGGC AAAGGTCTAT ATCTGAACGT
    AGAGCTGCCA TGGAAAATCA GCGATCTTAT TCAATGGAAA GAACTCGAGA GGCTCAGGGA
    CCAAGTTCTT ATCCACAAAG GACCACAAAC CATAGTCCTC CTACCCCCCG GAGGAGTCCA
    ATACCCATTG ATAGACCAGA CGTGAGACGT ACTGACTCAC TAAGGAAACA ACACCACCTA
    GATCCTAGTT CTGCTGTAAG GAAAACGAAA CGGGAAAAAA TGGAAACAAT GTTAAGGAAT
    GATTCTCTCA GTTCTGACCA GTCAGAGTCA GTGAGACCTC CACCACCAAA GCCTCATAAA
    TCAAAGAAAG GAGGTAAAAT GCGCCAGGTT TCATTGAGCA GTTCAGAGGA GGAATTAGCT
    TCTACACCTG AATATACCAG TTGCGATGAT GTTGAGATTG AAAGTGAGAG TGTGAGTGAA
    AAAGGGGACA GTCAAAAGGG AAAAAGAAAA ACTAGTGAGC AGGCAGTTTT GTCGGACTCT
    AACACCAGGT CTGAGAGACA AAAGAAAATG ATGTACTTTG GAGGCCACTC TTTGGAAGAG
    GATTTGGAAT GGTCTGAGCC TCAGATTAAG GACTCTGGGG TAGATACGTG TAGTAGCACA
    ACCCTTAATG AGGAGCATAG CCATAGTGAT AAGCACCCTG TGACGTGGCA GCCATCTAAA
    GATGGAGATC GTTTAATTGG TCGCATTTTA TTAAATAAGC GTTTAAAAGA TGGAAGTGTA
    CCTCGAGATT CAGGAGCAAT GCTTGGCTTG AAGGTTGTAG GAGGAAAGAT GACTGAATCA
    GGTCGGCTCT GTGCATTTAT AACCAAAGTT AAAAGAGGAA GTTTAGCTGA TACTGTAGGA
    CATCTTAGAC CAGGTGATGA AGTACTAGAG TGGAATGGAA GACTATTACA AGGAGCCACA
    TTTGAAGAAG TATATAACAT CATCCTGGAA TCCAAACCTG AACCACAGGT GGAGCTTGTT
    GTTTCAAGGC CTATTGGAGA TATACCTAGA ATACCTGACA GCACACACAC ACAGCTGGAG
    TCCAGTTCTA GCTCATTCGA ATCTCAAAAA ATGGATCGTC CTTCTATTTC TGTTACATCT
    CCCATGAGTC CTGGCATGTT GAGGGATGTT CCACAGTTCT TATCTGGACA ACTTTCAATA
    AAACTGTGGT TTGACAAGGT TGGTCACCAA TTAATAGTTA CAATTTTGGG AGCAAAAGAC
    CTCCCTTCCA GGGAAGATGG GAGGCCAAGG AATCCTTATG TTAAAATTTA CTTTCTTCCA
    GACAGAAGTG ACAAAAACAA GAGAAGAACT AAAACAGTGA AGAAAACTTT GGAACCCAAA
    TGGAACCAAA CATTCATTTA TTCTCCAGTC CACCGAAGAG AGTTTCGGGA ACGGATGCTG
    GAGATTACCC TTTGGGATCA AGCTCGAGTT CGAGAGGAAG AAAGTGAATT TTTAGGAGAG
    ATTTTAATTG AATTAGAAAC AGCATTATTA GATGATGAGC CACATTGGTA CAAACTTCAG
    ACTCACGATG TTTCTTCATT GCCACTTCCC CATCCTTCAC CATATATGCC ACGACGACAA
    CTCCATGGAG AGAGCCCAAC ACGGAGGTTG CAAAGATCAA AGAGAATAAG TGATAGTGAG
    GTCTCTGATT ATGACTGTGA TGATGGAATT GGTGTAGTAT CAGATTATCG ACACAATGGT
    CGGGATCTTC AAAGCTCAAC ATTGTCAGTG CCAGAACAAG TAATGTCATC CAACCACTGT
    TCGCCGTCAG GGTCTCCACA CCGAGTAGAT GTTATAGGAA GGACTAGATC TTGGTCACCC
    AGTGTCCCTC CTCCACAGAG TCGAAATGTG GAGCAGGGAC TTCGAGGGAC ACGCTCTGCT
    ATAGGACATT ATAATACAAT TAGCCGAATG GATAGACACC GTGTCATGGA TGACCATTAT
    TCTCCAGACA GAGACAGGGA TTGTGAAGCA GCAGATAGAC AGCCATATCA CAGATCCAGA
    TCAACAGAAC AGCGGCCTCT CCTAGAGCGG ACCACCACCC GCTCCAGATC CACTGAACGT
    CCTGATACAA ACCTCATGAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT
    TCACCTGCCT TATCGAGGTC TCATCCTCGT ACTGGGTCTG TCCAAACAAG CCCATCAAGT
    ACTCCAGTGG CAGGACGAAG GGGCCGACAA CTCCCACAGC TTCCACCAAA GGGAACATTG
    GAGAGAAAAA CCGGAGGTAA AAAGCTACGG AGCACTGTCC AGAGAAGTAC AGAAACAGGC
    TTGGCTGTGG AAATGAGGAA CTGGATGACT CGACAAGCGA GCCGAGAATC TACCGACGGC
    AGCATGAACA GCTACAGCTC AGAAGGAAAT CTGATCTTCC CTGGTGTCCG CTTGGCCTCG
    GATAGCCAAT TCAGTGATTT TCTGGATGGC CTTGGCCCTG CCCAGCTAGT GGGGCGCCAA
    ACTCTGGCCA CACCCGCCAT GGGTGACATT CAGGTAGGAA TGATGGACAA AAAGGGACAG
    CTGGAGGTGG AAATAATTCG GGCCCGTGGC CTTGTTGTAA AACCAGGTTC CAAGACACTG
    CCAGCACCAT ACGTAAAGGT GTATCTATTA GATAATGGAG TCTGCATAGC CAAAAAGAAA
    ACAAAAGTGG CAAGAAAAAC GCTGGAACCC CTTTACCAGC AACTATTATC TTTTGAAGAA
    AGTCCCCAAG GAAAGGTTTT ACAGATCATT GTCTGGGGAG ACTATGGCCG CATGGATCAC
    AAATCCTTTA TGGGAGTGGC CCAGATACTT TTAGATGAAC TGGAGCTATC CAACATGGTG
    ATCGGCTGGT TCAAACTCTT CCCCCCTTCC TCCCTAGTAG ATCCAACCTT GGCCCCTCTG
    ACAAGAAGAG CTTCCCAATC ATCTCTGGAA AGTTCAACTG GACCTTCTTA CTCTCGTTCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.