PUM1 cDNA ORF clone, Bos taurus(cattle)

The following PUM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227448 XM_024976010.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227449 XM_024976019.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227450 XM_024976022.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227451 XM_024976031.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227451 XM_024976033.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227452 XM_024976043.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227452 XM_024976038.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227453 XM_024976052.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227454 XM_024976053.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227455 XM_024976058.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa88000 XM_015459546.2
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88001 XM_015459549.2
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa227438 XM_024975964.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa227439 XM_024975970.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227440 XM_024975975.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227441 XM_024975979.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227442 XM_024975984.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227443 XM_024975990.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227444 XM_024975997.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227445 XM_024976002.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227446 XM_024976005.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227447 XM_024976007.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa38270 NM_001193123.1
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16796 XM_005202942.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16796 XM_005202941.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16797 XM_005202943.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16798 XM_005202944.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16799 XM_005202945.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16800 XM_005202946.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16801 XM_005202947.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16802 XM_005202948.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa16803 XM_005202949.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa16804 XM_005202950.4
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa53174 XM_010802429.3
Latest version!
Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa16798 XM_005202944.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16797 XM_005202943.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16799 XM_005202945.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16801 XM_005202947.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16804 XM_005202950.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa88000 XM_015459546.1 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa16796 XM_005202941.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16796 XM_005202942.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16802 XM_005202948.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa53174 XM_010802429.2 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa16800 XM_005202946.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16803 XM_005202949.3 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa88001 XM_015459549.1 Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227448
    Clone ID Related Accession (Same CDS sequence) XM_024976010.1
    Accession Version XM_024976010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831778.1
    CDS131..3064
    Translation

    Target ORF information:

    RefSeq Version XM_024976010.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227449
    Clone ID Related Accession (Same CDS sequence) XM_024976019.1
    Accession Version XM_024976019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGCAGG TTTAGCTCCC
    GCTGCATTCG TCCCCAACCC ATATATCATC AGTGCTGCGC CCCCAGGGAC GGACCCCTAC
    ACAGCTGGAT TGGCTGCAGC AGCGACGCTA GGCCCGGCTG TGGTCCCTCC CCAGTATTAC
    GGAGTTACTC CCTGGGGAGT CTACCCTGCC AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT
    GCTGCAGCAA CAAATTCTGC TAATCAGCAG ACCACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACGC CGAATCAGAA CCAGCAGGGA
    CAGCAAGCCG ATCCACTGGT GGCAGCTGCA GCGGTGAATT CTGCCCTTGC GTTTGGACAA
    GGACTGGCTG CGGGCATGCC AGGTTATCCA GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGAGCTCC TGTTCGACTT
    GTAGCTCCCG CCCCAGTCAT CATTAGTTCC TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAACCTGGCA
    TCCAGCTCTT TCTACGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CCGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGACTCAC AAATGGCAGT
    GGAAGATACA TCTCTGCTGC TCCAGGCGCT GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTT TTTTCCTCCT CCCGTTTGCG ATACGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTTTGGAAG ATTTTCGAAA TAACCGATAT
    CCTAATTTAC AATTGCGGGA GATTGCTGGG CACATAATGG AGTTTTCCCA AGACCAGCAT
    GGTTCCAGAT TCATTCAGCT GAAACTTGAG CGTGCCACAC CAGCCGAACG CCAACTTGTC
    TTCAATGAAA TCCTTCAGGC CGCTTACCAG CTGATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAGAAGT TCTTTGAATT TGGCAGCCTT GAACAGAAGC TGGCTTTGGC TGAGCGGATT
    CGAGGCCACG TCCTGTCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831787.1
    CDS134..2977
    Translation

    Target ORF information:

    RefSeq Version XM_024976019.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGCAGG TTTAGCTCCC
    GCTGCATTCG TCCCCAACCC ATATATCATC AGTGCTGCGC CCCCAGGGAC GGACCCCTAC
    ACAGCTGGAT TGGCTGCAGC AGCGACGCTA GGCCCGGCTG TGGTCCCTCC CCAGTATTAC
    GGAGTTACTC CCTGGGGAGT CTACCCTGCC AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT
    GCTGCAGCAA CAAATTCTGC TAATCAGCAG ACCACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACGC CGAATCAGAA CCAGCAGGGA
    CAGCAAGCCG ATCCACTGGT GGCAGCTGCA GCGGTGAATT CTGCCCTTGC GTTTGGACAA
    GGACTGGCTG CGGGCATGCC AGGTTATCCA GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGAGCTCC TGTTCGACTT
    GTAGCTCCCG CCCCAGTCAT CATTAGTTCC TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAACCTGGCA
    TCCAGCTCTT TCTACGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CCGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGACTCAC AAATGGCAGT
    GGAAGATACA TCTCTGCTGC TCCAGGCGCT GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTT TTTTCCTCCT CCCGTTTGCG ATACGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTTTGGAAG ATTTTCGAAA TAACCGATAT
    CCTAATTTAC AATTGCGGGA GATTGCTGGG CACATAATGG AGTTTTCCCA AGACCAGCAT
    GGTTCCAGAT TCATTCAGCT GAAACTTGAG CGTGCCACAC CAGCCGAACG CCAACTTGTC
    TTCAATGAAA TCCTTCAGGC CGCTTACCAG CTGATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAGAAGT TCTTTGAATT TGGCAGCCTT GAACAGAAGC TGGCTTTGGC TGAGCGGATT
    CGAGGCCACG TCCTGTCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227450
    Clone ID Related Accession (Same CDS sequence) XM_024976022.1
    Accession Version XM_024976022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831790.1
    CDS134..2974
    Translation

    Target ORF information:

    RefSeq Version XM_024976022.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227451
    Clone ID Related Accession (Same CDS sequence) XM_024976033.1 , XM_024976031.1
    Accession Version XM_024976033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTA TGTTTTCAGC AGGTTTAGCT CCCGCTGCAT TCGTCCCCAA CCCATATATC
    ATCAGTGCTG CGCCCCCAGG GACGGACCCC TACACAGCTG GATTGGCTGC AGCAGCGACG
    CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT TACGGAGTTA CTCCCTGGGG AGTCTACCCT
    GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC GCTGCTGCAG CAACAAATTC TGCTAATCAG
    CAGACCACCC CACAGGCTCA GCAAGGGCAG CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG
    CGTCCTTTGA CGCCGAATCA GAACCAGCAG GGACAGCAAG CCGATCCACT GGTGGCAGCT
    GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA CAAGGACTGG CTGCGGGCAT GCCAGGTTAT
    CCAGTCTTGG CTCCTGCTGC TTACTATGAC CAAACCGGTG CCCTTGTGGT GAATGCAGGA
    GCGAGAAATG GTCTTGGAGC TCCTGTTCGA CTTGTAGCTC CCGCCCCAGT CATCATTAGT
    TCCTCAGCTG CACAAGCAGC TGTTGCAGCG GCCGCAGCTT CAGCAAATGG AGCAGCCGGT
    GGTCTTGCTG GAACAACAAA TGGACCATTT CGCCCTTTAG GAACTCAGCA GCCTCAGCCC
    CAGCCCCAGC AGCAGCCCAG TAACAACCTG GCATCCAGCT CTTTCTACGG CAACAACTCT
    CTGAGCAGCA ATTCACAGAG CAGCTCCCTC TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC
    ACATCCTTGG GATTTGGAAG TAGCAGTTCT CTCGGCGCCA CCCTGGGATC AGCCCTTGGA
    GGGTTTGGAA CAGCAGTTGC AAACTCCAAC ACTGGCAGTG GCTCCCGCCG TGACTCCCTG
    ACTGGCAGCA GTGACCTTTA TAAGAGGACA TCGAGCAGCT TGACCCCCAT TGGACACAGT
    TTTTATAACG GCCTTAGCTT TTCCTCCTCT CCTGGACCCG TGGGCATGCC TCTCCCTAGT
    CAGGGACCAG GACATTCACA GACACCACCT CCTTCCCTCT CTTCACATGG ATCCTCTTCA
    AGCTTAAACC TGGGAGGACT CACAAATGGC AGTGGAAGAT ACATCTCTGC TGCTCCAGGC
    GCTGAAGCCA AGTACCGCAG CGCAAGCAGT GCCTCCAGCC TCTTCAGCCC CAGCAGCACC
    CTTTTTTCCT CCTCCCGTTT GCGATACGGA ATGTCTGATG TCATGCCCTC TGGCAGGAGC
    AGGCTTTTGG AAGATTTTCG AAATAACCGA TATCCTAATT TACAATTGCG GGAGATTGCT
    GGGCACATAA TGGAGTTTTC CCAAGACCAG CATGGTTCCA GATTCATTCA GCTGAAACTT
    GAGCGTGCCA CACCAGCCGA ACGCCAACTT GTCTTCAATG AAATCCTTCA GGCCGCTTAC
    CAGCTGATGG TGGATGTATT TGGAAATTAC GTCATTCAGA AGTTCTTTGA ATTTGGCAGC
    CTTGAACAGA AGCTGGCTTT GGCTGAGCGG ATTCGAGGCC ACGTCCTGTC ATTGGCACTG
    CAGATGTATG GCTGCCGTGT TATCCAGAAA GCTCTTGAGT TTATTCCCTC AGACCAGCAG
    GTAATTAATG AAATGGTTCG GGAACTGGAT GGCCACGTCC TCAAGTGTGT GAAAGACCAG
    AATGGCAATC ACGTGGTGCA GAAGTGCATC GAATGTGTGC AGCCCCAGTC TCTGCAGTTC
    ATCATCGATG CGTTTAAGGG GCAGGTGTTT GCTTTGTCCA CGCATCCTTA TGGCTGCCGA
    GTGATTCAGA GGATTCTGGA GCACTGTCTC CCTGACCAGA CACTCCCTAT TTTAGAGGAG
    CTTCACCAGC ACACAGAGCA ACTTGTACAG GATCAGTATG GAAATTACGT AATCCAGCAT
    GTATTGGAGC ATGGTCGTCC TGAGGATAAA AGTAAAATTG TAGCAGAAAT TCGAGGCAAT
    GTACTTGTGT TGAGTCAGCA CAAATTTGCA AGCAATGTTG TGGAGAAGTG TGTCACTCAC
    GCCTCCCGTA CAGAGCGCGC TGTGCTTATT GACGAGGTGT GCACCATGAA CGATGGTCCC
    CACAGTGCCT TATACACCAT GATGAAGGAC CAGTATGCCA ACTACGTGGT CCAGAAGATG
    ATCGATGTGG CAGAGCCAGC CCAGAGGAAG ATCGTCATGC ACAAGATCCG GCCCCACATC
    GCGACTCTTC GCAAGTATAC TTACGGCAAG CACATCCTGG CCAAGCTGGA GAAATACTAC
    ATGAAGAATG GCGTTGACTT AGGGCCCATC TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831801.1
    CDS159..2735
    Translation

    Target ORF information:

    RefSeq Version XM_024976033.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTA TGTTTTCAGC AGGTTTAGCT CCCGCTGCAT TCGTCCCCAA CCCATATATC
    ATCAGTGCTG CGCCCCCAGG GACGGACCCC TACACAGCTG GATTGGCTGC AGCAGCGACG
    CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT TACGGAGTTA CTCCCTGGGG AGTCTACCCT
    GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC GCTGCTGCAG CAACAAATTC TGCTAATCAG
    CAGACCACCC CACAGGCTCA GCAAGGGCAG CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG
    CGTCCTTTGA CGCCGAATCA GAACCAGCAG GGACAGCAAG CCGATCCACT GGTGGCAGCT
    GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA CAAGGACTGG CTGCGGGCAT GCCAGGTTAT
    CCAGTCTTGG CTCCTGCTGC TTACTATGAC CAAACCGGTG CCCTTGTGGT GAATGCAGGA
    GCGAGAAATG GTCTTGGAGC TCCTGTTCGA CTTGTAGCTC CCGCCCCAGT CATCATTAGT
    TCCTCAGCTG CACAAGCAGC TGTTGCAGCG GCCGCAGCTT CAGCAAATGG AGCAGCCGGT
    GGTCTTGCTG GAACAACAAA TGGACCATTT CGCCCTTTAG GAACTCAGCA GCCTCAGCCC
    CAGCCCCAGC AGCAGCCCAG TAACAACCTG GCATCCAGCT CTTTCTACGG CAACAACTCT
    CTGAGCAGCA ATTCACAGAG CAGCTCCCTC TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC
    ACATCCTTGG GATTTGGAAG TAGCAGTTCT CTCGGCGCCA CCCTGGGATC AGCCCTTGGA
    GGGTTTGGAA CAGCAGTTGC AAACTCCAAC ACTGGCAGTG GCTCCCGCCG TGACTCCCTG
    ACTGGCAGCA GTGACCTTTA TAAGAGGACA TCGAGCAGCT TGACCCCCAT TGGACACAGT
    TTTTATAACG GCCTTAGCTT TTCCTCCTCT CCTGGACCCG TGGGCATGCC TCTCCCTAGT
    CAGGGACCAG GACATTCACA GACACCACCT CCTTCCCTCT CTTCACATGG ATCCTCTTCA
    AGCTTAAACC TGGGAGGACT CACAAATGGC AGTGGAAGAT ACATCTCTGC TGCTCCAGGC
    GCTGAAGCCA AGTACCGCAG CGCAAGCAGT GCCTCCAGCC TCTTCAGCCC CAGCAGCACC
    CTTTTTTCCT CCTCCCGTTT GCGATACGGA ATGTCTGATG TCATGCCCTC TGGCAGGAGC
    AGGCTTTTGG AAGATTTTCG AAATAACCGA TATCCTAATT TACAATTGCG GGAGATTGCT
    GGGCACATAA TGGAGTTTTC CCAAGACCAG CATGGTTCCA GATTCATTCA GCTGAAACTT
    GAGCGTGCCA CACCAGCCGA ACGCCAACTT GTCTTCAATG AAATCCTTCA GGCCGCTTAC
    CAGCTGATGG TGGATGTATT TGGAAATTAC GTCATTCAGA AGTTCTTTGA ATTTGGCAGC
    CTTGAACAGA AGCTGGCTTT GGCTGAGCGG ATTCGAGGCC ACGTCCTGTC ATTGGCACTG
    CAGATGTATG GCTGCCGTGT TATCCAGAAA GCTCTTGAGT TTATTCCCTC AGACCAGCAG
    GTAATTAATG AAATGGTTCG GGAACTGGAT GGCCACGTCC TCAAGTGTGT GAAAGACCAG
    AATGGCAATC ACGTGGTGCA GAAGTGCATC GAATGTGTGC AGCCCCAGTC TCTGCAGTTC
    ATCATCGATG CGTTTAAGGG GCAGGTGTTT GCTTTGTCCA CGCATCCTTA TGGCTGCCGA
    GTGATTCAGA GGATTCTGGA GCACTGTCTC CCTGACCAGA CACTCCCTAT TTTAGAGGAG
    CTTCACCAGC ACACAGAGCA ACTTGTACAG GATCAGTATG GAAATTACGT AATCCAGCAT
    GTATTGGAGC ATGGTCGTCC TGAGGATAAA AGTAAAATTG TAGCAGAAAT TCGAGGCAAT
    GTACTTGTGT TGAGTCAGCA CAAATTTGCA AGCAATGTTG TGGAGAAGTG TGTCACTCAC
    GCCTCCCGTA CAGAGCGCGC TGTGCTTATT GACGAGGTGT GCACCATGAA CGATGGTCCC
    CACAGTGCCT TATACACCAT GATGAAGGAC CAGTATGCCA ACTACGTGGT CCAGAAGATG
    ATCGATGTGG CAGAGCCAGC CCAGAGGAAG ATCGTCATGC ACAAGATCCG GCCCCACATC
    GCGACTCTTC GCAAGTATAC TTACGGCAAG CACATCCTGG CCAAGCTGGA GAAATACTAC
    ATGAAGAATG GCGTTGACTT AGGGCCCATC TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227451
    Clone ID Related Accession (Same CDS sequence) XM_024976033.1 , XM_024976031.1
    Accession Version XM_024976031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTA TGTTTTCAGC AGGTTTAGCT CCCGCTGCAT TCGTCCCCAA CCCATATATC
    ATCAGTGCTG CGCCCCCAGG GACGGACCCC TACACAGCTG GATTGGCTGC AGCAGCGACG
    CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT TACGGAGTTA CTCCCTGGGG AGTCTACCCT
    GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC GCTGCTGCAG CAACAAATTC TGCTAATCAG
    CAGACCACCC CACAGGCTCA GCAAGGGCAG CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG
    CGTCCTTTGA CGCCGAATCA GAACCAGCAG GGACAGCAAG CCGATCCACT GGTGGCAGCT
    GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA CAAGGACTGG CTGCGGGCAT GCCAGGTTAT
    CCAGTCTTGG CTCCTGCTGC TTACTATGAC CAAACCGGTG CCCTTGTGGT GAATGCAGGA
    GCGAGAAATG GTCTTGGAGC TCCTGTTCGA CTTGTAGCTC CCGCCCCAGT CATCATTAGT
    TCCTCAGCTG CACAAGCAGC TGTTGCAGCG GCCGCAGCTT CAGCAAATGG AGCAGCCGGT
    GGTCTTGCTG GAACAACAAA TGGACCATTT CGCCCTTTAG GAACTCAGCA GCCTCAGCCC
    CAGCCCCAGC AGCAGCCCAG TAACAACCTG GCATCCAGCT CTTTCTACGG CAACAACTCT
    CTGAGCAGCA ATTCACAGAG CAGCTCCCTC TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC
    ACATCCTTGG GATTTGGAAG TAGCAGTTCT CTCGGCGCCA CCCTGGGATC AGCCCTTGGA
    GGGTTTGGAA CAGCAGTTGC AAACTCCAAC ACTGGCAGTG GCTCCCGCCG TGACTCCCTG
    ACTGGCAGCA GTGACCTTTA TAAGAGGACA TCGAGCAGCT TGACCCCCAT TGGACACAGT
    TTTTATAACG GCCTTAGCTT TTCCTCCTCT CCTGGACCCG TGGGCATGCC TCTCCCTAGT
    CAGGGACCAG GACATTCACA GACACCACCT CCTTCCCTCT CTTCACATGG ATCCTCTTCA
    AGCTTAAACC TGGGAGGACT CACAAATGGC AGTGGAAGAT ACATCTCTGC TGCTCCAGGC
    GCTGAAGCCA AGTACCGCAG CGCAAGCAGT GCCTCCAGCC TCTTCAGCCC CAGCAGCACC
    CTTTTTTCCT CCTCCCGTTT GCGATACGGA ATGTCTGATG TCATGCCCTC TGGCAGGAGC
    AGGCTTTTGG AAGATTTTCG AAATAACCGA TATCCTAATT TACAATTGCG GGAGATTGCT
    GGGCACATAA TGGAGTTTTC CCAAGACCAG CATGGTTCCA GATTCATTCA GCTGAAACTT
    GAGCGTGCCA CACCAGCCGA ACGCCAACTT GTCTTCAATG AAATCCTTCA GGCCGCTTAC
    CAGCTGATGG TGGATGTATT TGGAAATTAC GTCATTCAGA AGTTCTTTGA ATTTGGCAGC
    CTTGAACAGA AGCTGGCTTT GGCTGAGCGG ATTCGAGGCC ACGTCCTGTC ATTGGCACTG
    CAGATGTATG GCTGCCGTGT TATCCAGAAA GCTCTTGAGT TTATTCCCTC AGACCAGCAG
    GTAATTAATG AAATGGTTCG GGAACTGGAT GGCCACGTCC TCAAGTGTGT GAAAGACCAG
    AATGGCAATC ACGTGGTGCA GAAGTGCATC GAATGTGTGC AGCCCCAGTC TCTGCAGTTC
    ATCATCGATG CGTTTAAGGG GCAGGTGTTT GCTTTGTCCA CGCATCCTTA TGGCTGCCGA
    GTGATTCAGA GGATTCTGGA GCACTGTCTC CCTGACCAGA CACTCCCTAT TTTAGAGGAG
    CTTCACCAGC ACACAGAGCA ACTTGTACAG GATCAGTATG GAAATTACGT AATCCAGCAT
    GTATTGGAGC ATGGTCGTCC TGAGGATAAA AGTAAAATTG TAGCAGAAAT TCGAGGCAAT
    GTACTTGTGT TGAGTCAGCA CAAATTTGCA AGCAATGTTG TGGAGAAGTG TGTCACTCAC
    GCCTCCCGTA CAGAGCGCGC TGTGCTTATT GACGAGGTGT GCACCATGAA CGATGGTCCC
    CACAGTGCCT TATACACCAT GATGAAGGAC CAGTATGCCA ACTACGTGGT CCAGAAGATG
    ATCGATGTGG CAGAGCCAGC CCAGAGGAAG ATCGTCATGC ACAAGATCCG GCCCCACATC
    GCGACTCTTC GCAAGTATAC TTACGGCAAG CACATCCTGG CCAAGCTGGA GAAATACTAC
    ATGAAGAATG GCGTTGACTT AGGGCCCATC TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831799.1
    CDS507..3083
    Translation

    Target ORF information:

    RefSeq Version XM_024976031.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTA TGTTTTCAGC AGGTTTAGCT CCCGCTGCAT TCGTCCCCAA CCCATATATC
    ATCAGTGCTG CGCCCCCAGG GACGGACCCC TACACAGCTG GATTGGCTGC AGCAGCGACG
    CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT TACGGAGTTA CTCCCTGGGG AGTCTACCCT
    GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC GCTGCTGCAG CAACAAATTC TGCTAATCAG
    CAGACCACCC CACAGGCTCA GCAAGGGCAG CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG
    CGTCCTTTGA CGCCGAATCA GAACCAGCAG GGACAGCAAG CCGATCCACT GGTGGCAGCT
    GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA CAAGGACTGG CTGCGGGCAT GCCAGGTTAT
    CCAGTCTTGG CTCCTGCTGC TTACTATGAC CAAACCGGTG CCCTTGTGGT GAATGCAGGA
    GCGAGAAATG GTCTTGGAGC TCCTGTTCGA CTTGTAGCTC CCGCCCCAGT CATCATTAGT
    TCCTCAGCTG CACAAGCAGC TGTTGCAGCG GCCGCAGCTT CAGCAAATGG AGCAGCCGGT
    GGTCTTGCTG GAACAACAAA TGGACCATTT CGCCCTTTAG GAACTCAGCA GCCTCAGCCC
    CAGCCCCAGC AGCAGCCCAG TAACAACCTG GCATCCAGCT CTTTCTACGG CAACAACTCT
    CTGAGCAGCA ATTCACAGAG CAGCTCCCTC TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC
    ACATCCTTGG GATTTGGAAG TAGCAGTTCT CTCGGCGCCA CCCTGGGATC AGCCCTTGGA
    GGGTTTGGAA CAGCAGTTGC AAACTCCAAC ACTGGCAGTG GCTCCCGCCG TGACTCCCTG
    ACTGGCAGCA GTGACCTTTA TAAGAGGACA TCGAGCAGCT TGACCCCCAT TGGACACAGT
    TTTTATAACG GCCTTAGCTT TTCCTCCTCT CCTGGACCCG TGGGCATGCC TCTCCCTAGT
    CAGGGACCAG GACATTCACA GACACCACCT CCTTCCCTCT CTTCACATGG ATCCTCTTCA
    AGCTTAAACC TGGGAGGACT CACAAATGGC AGTGGAAGAT ACATCTCTGC TGCTCCAGGC
    GCTGAAGCCA AGTACCGCAG CGCAAGCAGT GCCTCCAGCC TCTTCAGCCC CAGCAGCACC
    CTTTTTTCCT CCTCCCGTTT GCGATACGGA ATGTCTGATG TCATGCCCTC TGGCAGGAGC
    AGGCTTTTGG AAGATTTTCG AAATAACCGA TATCCTAATT TACAATTGCG GGAGATTGCT
    GGGCACATAA TGGAGTTTTC CCAAGACCAG CATGGTTCCA GATTCATTCA GCTGAAACTT
    GAGCGTGCCA CACCAGCCGA ACGCCAACTT GTCTTCAATG AAATCCTTCA GGCCGCTTAC
    CAGCTGATGG TGGATGTATT TGGAAATTAC GTCATTCAGA AGTTCTTTGA ATTTGGCAGC
    CTTGAACAGA AGCTGGCTTT GGCTGAGCGG ATTCGAGGCC ACGTCCTGTC ATTGGCACTG
    CAGATGTATG GCTGCCGTGT TATCCAGAAA GCTCTTGAGT TTATTCCCTC AGACCAGCAG
    GTAATTAATG AAATGGTTCG GGAACTGGAT GGCCACGTCC TCAAGTGTGT GAAAGACCAG
    AATGGCAATC ACGTGGTGCA GAAGTGCATC GAATGTGTGC AGCCCCAGTC TCTGCAGTTC
    ATCATCGATG CGTTTAAGGG GCAGGTGTTT GCTTTGTCCA CGCATCCTTA TGGCTGCCGA
    GTGATTCAGA GGATTCTGGA GCACTGTCTC CCTGACCAGA CACTCCCTAT TTTAGAGGAG
    CTTCACCAGC ACACAGAGCA ACTTGTACAG GATCAGTATG GAAATTACGT AATCCAGCAT
    GTATTGGAGC ATGGTCGTCC TGAGGATAAA AGTAAAATTG TAGCAGAAAT TCGAGGCAAT
    GTACTTGTGT TGAGTCAGCA CAAATTTGCA AGCAATGTTG TGGAGAAGTG TGTCACTCAC
    GCCTCCCGTA CAGAGCGCGC TGTGCTTATT GACGAGGTGT GCACCATGAA CGATGGTCCC
    CACAGTGCCT TATACACCAT GATGAAGGAC CAGTATGCCA ACTACGTGGT CCAGAAGATG
    ATCGATGTGG CAGAGCCAGC CCAGAGGAAG ATCGTCATGC ACAAGATCCG GCCCCACATC
    GCGACTCTTC GCAAGTATAC TTACGGCAAG CACATCCTGG CCAAGCTGGA GAAATACTAC
    ATGAAGAATG GCGTTGACTT AGGGCCCATC TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227452
    Clone ID Related Accession (Same CDS sequence) XM_024976038.1 , XM_024976043.1
    Accession Version XM_024976038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCTCTTG CCAGGTAAAA 
    AGTTTTGGGA AACAGATGAA TCCAGCAAAG ATGGACCAAA AGGAATATTC CTGGGTGATC
    AATGGCGAGA CAGTGCCTGG GGAACATCAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831806.1
    CDS154..2727
    Translation

    Target ORF information:

    RefSeq Version XM_024976038.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCTCTTG CCAGGTAAAA 
    AGTTTTGGGA AACAGATGAA TCCAGCAAAG ATGGACCAAA AGGAATATTC CTGGGTGATC
    AATGGCGAGA CAGTGCCTGG GGAACATCAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227452
    Clone ID Related Accession (Same CDS sequence) XM_024976038.1 , XM_024976043.1
    Accession Version XM_024976043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCTCTTG CCAGGTAAAA 
    AGTTTTGGGA AACAGATGAA TCCAGCAAAG ATGGACCAAA AGGAATATTC CTGGGTGATC
    AATGGCGAGA CAGTGCCTGG GGAACATCAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831811.1
    CDS159..2732
    Translation

    Target ORF information:

    RefSeq Version XM_024976043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCTCTTG CCAGGTAAAA 
    AGTTTTGGGA AACAGATGAA TCCAGCAAAG ATGGACCAAA AGGAATATTC CTGGGTGATC
    AATGGCGAGA CAGTGCCTGG GGAACATCAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227453
    Clone ID Related Accession (Same CDS sequence) XM_024976052.1
    Accession Version XM_024976052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGCAG GTTTAGCTCC CGCTGCATTC GTCCCCAACC CATATATCAT CAGTGCTGCG
    CCCCCAGGGA CGGACCCCTA CACAGCTGGA TTGGCTGCAG CAGCGACGCT AGGCCCGGCT
    GTGGTCCCTC CCCAGTATTA CGGAGTTACT CCCTGGGGAG TCTACCCTGC CAGTCTTTTC
    CAGCAGCAAG CCGCCGCCGC TGCTGCAGCA ACAAATTCTG CTAATCAGCA GACCACCCCA
    CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC CGTGGAGGAG CCAGCCAGCG TCCTTTGACG
    CCGAATCAGA ACCAGCAGGG ACAGCAAGCC GATCCACTGG TGGCAGCTGC AGCGGTGAAT
    TCTGCCCTTG CGTTTGGACA AGGACTGGCT GCGGGCATGC CAGGTTATCC AGTCTTGGCT
    CCTGCTGCTT ACTATGACCA AACCGGTGCC CTTGTGGTGA ATGCAGGAGC GAGAAATGGT
    CTTGGAGCTC CTGTTCGACT TGTAGCTCCC GCCCCAGTCA TCATTAGTTC CTCAGCTGCA
    CAAGCAGCTG TTGCAGCGGC CGCAGCTTCA GCAAATGGAG CAGCCGGTGG TCTTGCTGGA
    ACAACAAATG GACCATTTCG CCCTTTAGGA ACTCAGCAGC CTCAGCCCCA GCCCCAGCAG
    CAGCCCAGTA ACAACCTGGC ATCCAGCTCT TTCTACGGCA ACAACTCTCT GAGCAGCAAT
    TCACAGAGCA GCTCCCTCTT CTCCCAGGGC TCCGCCCAGC CTGCCAACAC ATCCTTGGGA
    TTTGGAAGTA GCAGTTCTCT CGGCGCCACC CTGGGATCAG CCCTTGGAGG GTTTGGAACA
    GCAGTTGCAA ACTCCAACAC TGGCAGTGGC TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT
    GACCTTTATA AGAGGACATC GAGCAGCTTG ACCCCCATTG GACACAGTTT TTATAACGGC
    CTTAGCTTTT CCTCCTCTCC TGGACCCGTG GGCATGCCTC TCCCTAGTCA GGGACCAGGA
    CATTCACAGA CACCACCTCC TTCCCTCTCT TCACATGGAT CCTCTTCAAG CTTAAACCTG
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831820.1
    CDS507..3071
    Translation

    Target ORF information:

    RefSeq Version XM_024976052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGCAG GTTTAGCTCC CGCTGCATTC GTCCCCAACC CATATATCAT CAGTGCTGCG
    CCCCCAGGGA CGGACCCCTA CACAGCTGGA TTGGCTGCAG CAGCGACGCT AGGCCCGGCT
    GTGGTCCCTC CCCAGTATTA CGGAGTTACT CCCTGGGGAG TCTACCCTGC CAGTCTTTTC
    CAGCAGCAAG CCGCCGCCGC TGCTGCAGCA ACAAATTCTG CTAATCAGCA GACCACCCCA
    CAGGCTCAGC AAGGGCAGCA GCAGGTTCTC CGTGGAGGAG CCAGCCAGCG TCCTTTGACG
    CCGAATCAGA ACCAGCAGGG ACAGCAAGCC GATCCACTGG TGGCAGCTGC AGCGGTGAAT
    TCTGCCCTTG CGTTTGGACA AGGACTGGCT GCGGGCATGC CAGGTTATCC AGTCTTGGCT
    CCTGCTGCTT ACTATGACCA AACCGGTGCC CTTGTGGTGA ATGCAGGAGC GAGAAATGGT
    CTTGGAGCTC CTGTTCGACT TGTAGCTCCC GCCCCAGTCA TCATTAGTTC CTCAGCTGCA
    CAAGCAGCTG TTGCAGCGGC CGCAGCTTCA GCAAATGGAG CAGCCGGTGG TCTTGCTGGA
    ACAACAAATG GACCATTTCG CCCTTTAGGA ACTCAGCAGC CTCAGCCCCA GCCCCAGCAG
    CAGCCCAGTA ACAACCTGGC ATCCAGCTCT TTCTACGGCA ACAACTCTCT GAGCAGCAAT
    TCACAGAGCA GCTCCCTCTT CTCCCAGGGC TCCGCCCAGC CTGCCAACAC ATCCTTGGGA
    TTTGGAAGTA GCAGTTCTCT CGGCGCCACC CTGGGATCAG CCCTTGGAGG GTTTGGAACA
    GCAGTTGCAA ACTCCAACAC TGGCAGTGGC TCCCGCCGTG ACTCCCTGAC TGGCAGCAGT
    GACCTTTATA AGAGGACATC GAGCAGCTTG ACCCCCATTG GACACAGTTT TTATAACGGC
    CTTAGCTTTT CCTCCTCTCC TGGACCCGTG GGCATGCCTC TCCCTAGTCA GGGACCAGGA
    CATTCACAGA CACCACCTCC TTCCCTCTCT TCACATGGAT CCTCTTCAAG CTTAAACCTG
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227454
    Clone ID Related Accession (Same CDS sequence) XM_024976053.1
    Accession Version XM_024976053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831821.1
    CDS507..3068
    Translation

    Target ORF information:

    RefSeq Version XM_024976053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATTTTCA AGCACTTGCT CTGGAGGGAA GAGCGATGGG AGAGCAGCTC TTGCCAGGTA 
    AAAAGTTTTG GGAAACAGAT GAATCCAGCA AAGATGGACC AAAAGGAATA TTCCTGGGTG
    ATCAATGGCG AGACAGTGCC TGGGGAACAT CAGCTGTTCC AAAGACCCAA TGCGCTTGCT
    GTCCAGCAGT TGACAGCCGC TCAGCAGCAG CAGTATGCGT TAGCAGCCGC GCATCAACCC
    CACATCGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227455
    Clone ID Related Accession (Same CDS sequence) XM_024976058.1
    Accession Version XM_024976058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831826.1
    CDS131..2626
    Translation

    Target ORF information:

    RefSeq Version XM_024976058.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88000
    Clone ID Related Accession (Same CDS sequence) XM_015459546.2 , XM_015459546.1
    Accession Version XM_015459546.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315032.1
    CDS134..2989
    Translation

    Target ORF information:

    RefSeq Version XM_015459546.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459546.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88000
    Clone ID Related Accession (Same CDS sequence) XM_015459546.2 , XM_015459546.1
    Accession Version XM_015459546.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315032.1
    CDS118..2973
    Translation

    Target ORF information:

    RefSeq Version XM_015459546.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTAT GTTTTCAGCA
    GGTTTAGCTC CCGCTGCATT CGTCCCCAAC CCATATATCA TCAGTGCTGC GCCCCCAGGG
    ACGGACCCCT ACACAGCTGG ATTGGCTGCA GCAGCGACGC TAGGCCCGGC TGTGGTCCCT
    CCCCAGTATT ACGGAGTTAC TCCCTGGGGA GTCTACCCTG CCAGTCTTTT CCAGCAGCAA
    GCCGCCGCCG CTGCTGCAGC AACAAATTCT GCTAATCAGC AGACCACCCC ACAGGCTCAG
    CAAGGGCAGC AGCAGGTTCT CCGTGGAGGA GCCAGCCAGC GTCCTTTGAC GCCGAATCAG
    AACCAGCAGG GACAGCAAGC CGATCCACTG GTGGCAGCTG CAGCGGTGAA TTCTGCCCTT
    GCGTTTGGAC AAGGACTGGC TGCGGGCATG CCAGGTTATC CAGTCTTGGC TCCTGCTGCT
    TACTATGACC AAACCGGTGC CCTTGTGGTG AATGCAGGAG CGAGAAATGG TCTTGGAGCT
    CCTGTTCGAC TTGTAGCTCC CGCCCCAGTC ATCATTAGTT CCTCAGCTGC ACAAGCAGCT
    GTTGCAGCGG CCGCAGCTTC AGCAAATGGA GCAGCCGGTG GTCTTGCTGG AACAACAAAT
    GGACCATTTC GCCCTTTAGG AACTCAGCAG CCTCAGCCCC AGCCCCAGCA GCAGCCCAGT
    AACAACCTGG CATCCAGCTC TTTCTACGGC AACAACTCTC TGAGCAGCAA TTCACAGAGC
    AGCTCCCTCT TCTCCCAGGG CTCCGCCCAG CCTGCCAACA CATCCTTGGG ATTTGGAAGT
    AGCAGTTCTC TCGGCGCCAC CCTGGGATCA GCCCTTGGAG GGTTTGGAAC AGCAGTTGCA
    AACTCCAACA CTGGCAGTGG CTCCCGCCGT GACTCCCTGA CTGGCAGCAG TGACCTTTAT
    AAGAGGACAT CGAGCAGCTT GACCCCCATT GGACACAGTT TTTATAACGG CCTTAGCTTT
    TCCTCCTCTC CTGGACCCGT GGGCATGCCT CTCCCTAGTC AGGGACCAGG ACATTCACAG
    ACACCACCTC CTTCCCTCTC TTCACATGGA TCCTCTTCAA GCTTAAACCT GGGAGGACTC
    ACAAATGGCA GTGGAAGATA CATCTCTGCT GCTCCAGGCG CTGAAGCCAA GTACCGCAGC
    GCAAGCAGTG CCTCCAGCCT CTTCAGCCCC AGCAGCACCC TTTTTTCCTC CTCCCGTTTG
    CGATACGGAA TGTCTGATGT CATGCCCTCT GGCAGGAGCA GGCTTTTGGA AGATTTTCGA
    AATAACCGAT ATCCTAATTT ACAATTGCGG GAGATTGCTG GGCACATAAT GGAGTTTTCC
    CAAGACCAGC ATGGTTCCAG ATTCATTCAG CTGAAACTTG AGCGTGCCAC ACCAGCCGAA
    CGCCAACTTG TCTTCAATGA AATCCTTCAG GCCGCTTACC AGCTGATGGT GGATGTATTT
    GGAAATTACG TCATTCAGAA GTTCTTTGAA TTTGGCAGCC TTGAACAGAA GCTGGCTTTG
    GCTGAGCGGA TTCGAGGCCA CGTCCTGTCA TTGGCACTGC AGATGTATGG CTGCCGTGTT
    ATCCAGAAAG CTCTTGAGTT TATTCCCTCA GACCAGCAGG TAATTAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88001
    Clone ID Related Accession (Same CDS sequence) XM_015459549.1 , XM_015459549.2
    Accession Version XM_015459549.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGAATGAA ATGGTTCGGG AACTGGATGG CCACGTCCTC
    AAGTGTGTGA AAGACCAGAA TGGCAATCAC GTGGTGCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTC TGCAGTTCAT CATCGATGCG TTTAAGGGGC AGGTGTTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATTCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTACGTAA TCCAGCATGT ATTGGAGCAT GGTCGTCCTG AGGATAAAAG TAAAATTGTA
    GCAGAAATTC GAGGCAATGT ACTTGTGTTG AGTCAGCACA AATTTGCAAG CAATGTTGTG
    GAGAAGTGTG TCACTCACGC CTCCCGTACA GAGCGCGCTG TGCTTATTGA CGAGGTGTGC
    ACCATGAACG ATGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGAGGAAGAT CGTCATGCAC
    AAGATCCGGC CCCACATCGC GACTCTTCGC AAGTATACTT ACGGCAAGCA CATCCTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG CGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315035.1
    CDS117..2951
    Translation

    Target ORF information:

    RefSeq Version XM_015459549.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGAATGAA ATGGTTCGGG AACTGGATGG CCACGTCCTC
    AAGTGTGTGA AAGACCAGAA TGGCAATCAC GTGGTGCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTC TGCAGTTCAT CATCGATGCG TTTAAGGGGC AGGTGTTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATTCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTACGTAA TCCAGCATGT ATTGGAGCAT GGTCGTCCTG AGGATAAAAG TAAAATTGTA
    GCAGAAATTC GAGGCAATGT ACTTGTGTTG AGTCAGCACA AATTTGCAAG CAATGTTGTG
    GAGAAGTGTG TCACTCACGC CTCCCGTACA GAGCGCGCTG TGCTTATTGA CGAGGTGTGC
    ACCATGAACG ATGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGAGGAAGAT CGTCATGCAC
    AAGATCCGGC CCCACATCGC GACTCTTCGC AAGTATACTT ACGGCAAGCA CATCCTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG CGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88001
    Clone ID Related Accession (Same CDS sequence) XM_015459549.1 , XM_015459549.2
    Accession Version XM_015459549.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGAATGAA ATGGTTCGGG AACTGGATGG CCACGTCCTC
    AAGTGTGTGA AAGACCAGAA TGGCAATCAC GTGGTGCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTC TGCAGTTCAT CATCGATGCG TTTAAGGGGC AGGTGTTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATTCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTACGTAA TCCAGCATGT ATTGGAGCAT GGTCGTCCTG AGGATAAAAG TAAAATTGTA
    GCAGAAATTC GAGGCAATGT ACTTGTGTTG AGTCAGCACA AATTTGCAAG CAATGTTGTG
    GAGAAGTGTG TCACTCACGC CTCCCGTACA GAGCGCGCTG TGCTTATTGA CGAGGTGTGC
    ACCATGAACG ATGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGAGGAAGAT CGTCATGCAC
    AAGATCCGGC CCCACATCGC GACTCTTCGC AAGTATACTT ACGGCAAGCA CATCCTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG CGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315035.1
    CDS134..2968
    Translation

    Target ORF information:

    RefSeq Version XM_015459549.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459549.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTGTTCCA AAGACCCAAT GCGCTTGCTG TCCAGCAGTT GACAGCCGCT
    CAGCAGCAGC AGTATGCGTT AGCAGCCGCG CATCAACCCC ACATCGGTTT AGCTCCCGCT
    GCATTCGTCC CCAACCCATA TATCATCAGT GCTGCGCCCC CAGGGACGGA CCCCTACACA
    GCTGGATTGG CTGCAGCAGC GACGCTAGGC CCGGCTGTGG TCCCTCCCCA GTATTACGGA
    GTTACTCCCT GGGGAGTCTA CCCTGCCAGT CTTTTCCAGC AGCAAGCCGC CGCCGCTGCT
    GCAGCAACAA ATTCTGCTAA TCAGCAGACC ACCCCACAGG CTCAGCAAGG GCAGCAGCAG
    GTTCTCCGTG GAGGAGCCAG CCAGCGTCCT TTGACGCCGA ATCAGAACCA GCAGGGACAG
    CAAGCCGATC CACTGGTGGC AGCTGCAGCG GTGAATTCTG CCCTTGCGTT TGGACAAGGA
    CTGGCTGCGG GCATGCCAGG TTATCCAGTC TTGGCTCCTG CTGCTTACTA TGACCAAACC
    GGTGCCCTTG TGGTGAATGC AGGAGCGAGA AATGGTCTTG GAGCTCCTGT TCGACTTGTA
    GCTCCCGCCC CAGTCATCAT TAGTTCCTCA GCTGCACAAG CAGCTGTTGC AGCGGCCGCA
    GCTTCAGCAA ATGGAGCAGC CGGTGGTCTT GCTGGAACAA CAAATGGACC ATTTCGCCCT
    TTAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGAATGAA ATGGTTCGGG AACTGGATGG CCACGTCCTC
    AAGTGTGTGA AAGACCAGAA TGGCAATCAC GTGGTGCAGA AGTGCATCGA ATGTGTGCAG
    CCCCAGTCTC TGCAGTTCAT CATCGATGCG TTTAAGGGGC AGGTGTTTGC TTTGTCCACG
    CATCCTTATG GCTGCCGAGT GATTCAGAGG ATTCTGGAGC ACTGTCTCCC TGACCAGACA
    CTCCCTATTT TAGAGGAGCT TCACCAGCAC ACAGAGCAAC TTGTACAGGA TCAGTATGGA
    AATTACGTAA TCCAGCATGT ATTGGAGCAT GGTCGTCCTG AGGATAAAAG TAAAATTGTA
    GCAGAAATTC GAGGCAATGT ACTTGTGTTG AGTCAGCACA AATTTGCAAG CAATGTTGTG
    GAGAAGTGTG TCACTCACGC CTCCCGTACA GAGCGCGCTG TGCTTATTGA CGAGGTGTGC
    ACCATGAACG ATGGTCCCCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAC
    TACGTGGTCC AGAAGATGAT CGATGTGGCA GAGCCAGCCC AGAGGAAGAT CGTCATGCAC
    AAGATCCGGC CCCACATCGC GACTCTTCGC AAGTATACTT ACGGCAAGCA CATCCTGGCC
    AAGCTGGAGA AATACTACAT GAAGAATGGC GTTGACTTAG GGCCCATCTG CGGCCCCCCT
    AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227438
    Clone ID Related Accession (Same CDS sequence) XM_024975964.1
    Accession Version XM_024975964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831732.1
    CDS134..3697
    Translation

    Target ORF information:

    RefSeq Version XM_024975964.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227439
    Clone ID Related Accession (Same CDS sequence) XM_024975970.1
    Accession Version XM_024975970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGGAACTCAG
    CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAACC TGGCATCCAG CTCTTTCTAC
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGA CTCACAAATG GCAGTGGAAG ATACATCTCT
    GCTGCTCCAG GCGCTGAAGC CAAGTACCGC AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT TTGCGATACG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTTT GGAAGATTTT CGAAATAACC GATATCCTAA TTTACAATTG
    CGGGAGATTG CTGGGCACAT AATGGAGTTT TCCCAAGACC AGCATGGTTC CAGATTCATT
    CAGCTGAAAC TTGAGCGTGC CACACCAGCC GAACGCCAAC TTGTCTTCAA TGAAATCCTT
    CAGGCCGCTT ACCAGCTGAT GGTGGATGTA TTTGGAAATT ACGTCATTCA GAAGTTCTTT
    GAATTTGGCA GCCTTGAACA GAAGCTGGCT TTGGCTGAGC GGATTCGAGG CCACGTCCTG
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA GTTTATTCCC
    TCAGACCAGC AGAATGAAAT GGTTCGGGAA CTGGATGGCC ACGTCCTCAA GTGTGTGAAA
    GACCAGAATG GCAATCACGT GGTGCAGAAG TGCATCGAAT GTGTGCAGCC CCAGTCTCTG
    CAGTTCATCA TCGATGCGTT TAAGGGGCAG GTGTTTGCTT TGTCCACGCA TCCTTATGGC
    TGCCGAGTGA TTCAGAGGAT TCTGGAGCAC TGTCTCCCTG ACCAGACACT CCCTATTTTA
    GAGGAGCTTC ACCAGCACAC AGAGCAACTT GTACAGGATC AGTATGGAAA TTACGTAATC
    CAGCATGTAT TGGAGCATGG TCGTCCTGAG GATAAAAGTA AAATTGTAGC AGAAATTCGA
    GGCAATGTAC TTGTGTTGAG TCAGCACAAA TTTGCAAGCA ATGTTGTGGA GAAGTGTGTC
    ACTCACGCCT CCCGTACAGA GCGCGCTGTG CTTATTGACG AGGTGTGCAC CATGAACGAT
    GGTCCCCACA GTGCCTTATA CACCATGATG AAGGACCAGT ATGCCAACTA CGTGGTCCAG
    AAGATGATCG ATGTGGCAGA GCCAGCCCAG AGGAAGATCG TCATGCACAA GATCCGGCCC
    CACATCGCGA CTCTTCGCAA GTATACTTAC GGCAAGCACA TCCTGGCCAA GCTGGAGAAA
    TACTACATGA AGAATGGCGT TGACTTAGGG CCCATCTGCG GCCCCCCTAA TGGTATTATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831738.1
    CDS134..3616
    Translation

    Target ORF information:

    RefSeq Version XM_024975970.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGGAACTCAG
    CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAACC TGGCATCCAG CTCTTTCTAC
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGA CTCACAAATG GCAGTGGAAG ATACATCTCT
    GCTGCTCCAG GCGCTGAAGC CAAGTACCGC AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT TTGCGATACG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTTT GGAAGATTTT CGAAATAACC GATATCCTAA TTTACAATTG
    CGGGAGATTG CTGGGCACAT AATGGAGTTT TCCCAAGACC AGCATGGTTC CAGATTCATT
    CAGCTGAAAC TTGAGCGTGC CACACCAGCC GAACGCCAAC TTGTCTTCAA TGAAATCCTT
    CAGGCCGCTT ACCAGCTGAT GGTGGATGTA TTTGGAAATT ACGTCATTCA GAAGTTCTTT
    GAATTTGGCA GCCTTGAACA GAAGCTGGCT TTGGCTGAGC GGATTCGAGG CCACGTCCTG
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA GTTTATTCCC
    TCAGACCAGC AGAATGAAAT GGTTCGGGAA CTGGATGGCC ACGTCCTCAA GTGTGTGAAA
    GACCAGAATG GCAATCACGT GGTGCAGAAG TGCATCGAAT GTGTGCAGCC CCAGTCTCTG
    CAGTTCATCA TCGATGCGTT TAAGGGGCAG GTGTTTGCTT TGTCCACGCA TCCTTATGGC
    TGCCGAGTGA TTCAGAGGAT TCTGGAGCAC TGTCTCCCTG ACCAGACACT CCCTATTTTA
    GAGGAGCTTC ACCAGCACAC AGAGCAACTT GTACAGGATC AGTATGGAAA TTACGTAATC
    CAGCATGTAT TGGAGCATGG TCGTCCTGAG GATAAAAGTA AAATTGTAGC AGAAATTCGA
    GGCAATGTAC TTGTGTTGAG TCAGCACAAA TTTGCAAGCA ATGTTGTGGA GAAGTGTGTC
    ACTCACGCCT CCCGTACAGA GCGCGCTGTG CTTATTGACG AGGTGTGCAC CATGAACGAT
    GGTCCCCACA GTGCCTTATA CACCATGATG AAGGACCAGT ATGCCAACTA CGTGGTCCAG
    AAGATGATCG ATGTGGCAGA GCCAGCCCAG AGGAAGATCG TCATGCACAA GATCCGGCCC
    CACATCGCGA CTCTTCGCAA GTATACTTAC GGCAAGCACA TCCTGGCCAA GCTGGAGAAA
    TACTACATGA AGAATGGCGT TGACTTAGGG CCCATCTGCG GCCCCCCTAA TGGTATTATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227440
    Clone ID Related Accession (Same CDS sequence) XM_024975975.1
    Accession Version XM_024975975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831743.1
    CDS384..3605
    Translation

    Target ORF information:

    RefSeq Version XM_024975975.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227441
    Clone ID Related Accession (Same CDS sequence) XM_024975979.1
    Accession Version XM_024975979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831747.1
    CDS131..3349
    Translation

    Target ORF information:

    RefSeq Version XM_024975979.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA 
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227442
    Clone ID Related Accession (Same CDS sequence) XM_024975984.1
    Accession Version XM_024975984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGGTATGT TTTCAGCAGG TTTAGCTCCC
    GCTGCATTCG TCCCCAACCC ATATATCATC AGTGCTGCGC CCCCAGGGAC GGACCCCTAC
    ACAGCTGGAT TGGCTGCAGC AGCGACGCTA GGCCCGGCTG TGGTCCCTCC CCAGTATTAC
    GGAGTTACTC CCTGGGGAGT CTACCCTGCC AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT
    GCTGCAGCAA CAAATTCTGC TAATCAGCAG ACCACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACGC CGAATCAGAA CCAGCAGGGA
    CAGCAAGCCG ATCCACTGGT GGCAGCTGCA GCGGTGAATT CTGCCCTTGC GTTTGGACAA
    GGACTGGCTG CGGGCATGCC AGGTTATCCA GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGAGCTCC TGTTCGACTT
    GTAGCTCCCG CCCCAGTCAT CATTAGTTCC TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAACCTGGCA
    TCCAGCTCTT TCTACGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CCGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGACTCAC AAATGGCAGT
    GGAAGATACA TCTCTGCTGC TCCAGGCGCT GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTT TTTTCCTCCT CCCGTTTGCG ATACGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTTTGGAAG ATTTTCGAAA TAACCGATAT
    CCTAATTTAC AATTGCGGGA GATTGCTGGG CACATAATGG AGTTTTCCCA AGACCAGCAT
    GGTTCCAGAT TCATTCAGCT GAAACTTGAG CGTGCCACAC CAGCCGAACG CCAACTTGTC
    TTCAATGAAA TCCTTCAGGC CGCTTACCAG CTGATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAGAAGT TCTTTGAATT TGGCAGCCTT GAACAGAAGC TGGCTTTGGC TGAGCGGATT
    CGAGGCCACG TCCTGTCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831752.1
    CDS134..3277
    Translation

    Target ORF information:

    RefSeq Version XM_024975984.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGGTATGT TTTCAGCAGG TTTAGCTCCC
    GCTGCATTCG TCCCCAACCC ATATATCATC AGTGCTGCGC CCCCAGGGAC GGACCCCTAC
    ACAGCTGGAT TGGCTGCAGC AGCGACGCTA GGCCCGGCTG TGGTCCCTCC CCAGTATTAC
    GGAGTTACTC CCTGGGGAGT CTACCCTGCC AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT
    GCTGCAGCAA CAAATTCTGC TAATCAGCAG ACCACCCCAC AGGCTCAGCA AGGGCAGCAG
    CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT CCTTTGACGC CGAATCAGAA CCAGCAGGGA
    CAGCAAGCCG ATCCACTGGT GGCAGCTGCA GCGGTGAATT CTGCCCTTGC GTTTGGACAA
    GGACTGGCTG CGGGCATGCC AGGTTATCCA GTCTTGGCTC CTGCTGCTTA CTATGACCAA
    ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG AGAAATGGTC TTGGAGCTCC TGTTCGACTT
    GTAGCTCCCG CCCCAGTCAT CATTAGTTCC TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC
    GCAGCTTCAG CAAATGGAGC AGCCGGTGGT CTTGCTGGAA CAACAAATGG ACCATTTCGC
    CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG CCCCAGCAGC AGCCCAGTAA CAACCTGGCA
    TCCAGCTCTT TCTACGGCAA CAACTCTCTG AGCAGCAATT CACAGAGCAG CTCCCTCTTC
    TCCCAGGGCT CCGCCCAGCC TGCCAACACA TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC
    GGCGCCACCC TGGGATCAGC CCTTGGAGGG TTTGGAACAG CAGTTGCAAA CTCCAACACT
    GGCAGTGGCT CCCGCCGTGA CTCCCTGACT GGCAGCAGTG ACCTTTATAA GAGGACATCG
    AGCAGCTTGA CCCCCATTGG ACACAGTTTT TATAACGGCC TTAGCTTTTC CTCCTCTCCT
    GGACCCGTGG GCATGCCTCT CCCTAGTCAG GGACCAGGAC ATTCACAGAC ACCACCTCCT
    TCCCTCTCTT CACATGGATC CTCTTCAAGC TTAAACCTGG GAGGACTCAC AAATGGCAGT
    GGAAGATACA TCTCTGCTGC TCCAGGCGCT GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC
    TCCAGCCTCT TCAGCCCCAG CAGCACCCTT TTTTCCTCCT CCCGTTTGCG ATACGGAATG
    TCTGATGTCA TGCCCTCTGG CAGGAGCAGG CTTTTGGAAG ATTTTCGAAA TAACCGATAT
    CCTAATTTAC AATTGCGGGA GATTGCTGGG CACATAATGG AGTTTTCCCA AGACCAGCAT
    GGTTCCAGAT TCATTCAGCT GAAACTTGAG CGTGCCACAC CAGCCGAACG CCAACTTGTC
    TTCAATGAAA TCCTTCAGGC CGCTTACCAG CTGATGGTGG ATGTATTTGG AAATTACGTC
    ATTCAGAAGT TCTTTGAATT TGGCAGCCTT GAACAGAAGC TGGCTTTGGC TGAGCGGATT
    CGAGGCCACG TCCTGTCATT GGCACTGCAG ATGTATGGCT GCCGTGTTAT CCAGAAAGCT
    CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA ATTAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227443
    Clone ID Related Accession (Same CDS sequence) XM_024975990.1
    Accession Version XM_024975990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGCAGGTT TAGCTCCCGC TGCATTCGTC
    CCCAACCCAT ATATCATCAG TGCTGCGCCC CCAGGGACGG ACCCCTACAC AGCTGGATTG
    GCTGCAGCAG CGACGCTAGG CCCGGCTGTG GTCCCTCCCC AGTATTACGG AGTTACTCCC
    TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG CAGCAAGCCG CCGCCGCTGC TGCAGCAACA
    AATTCTGCTA ATCAGCAGAC CACCCCACAG GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT
    GGAGGAGCCA GCCAGCGTCC TTTGACGCCG AATCAGAACC AGCAGGGACA GCAAGCCGAT
    CCACTGGTGG CAGCTGCAGC GGTGAATTCT GCCCTTGCGT TTGGACAAGG ACTGGCTGCG
    GGCATGCCAG GTTATCCAGT CTTGGCTCCT GCTGCTTACT ATGACCAAAC CGGTGCCCTT
    GTGGTGAATG CAGGAGCGAG AAATGGTCTT GGAGCTCCTG TTCGACTTGT AGCTCCCGCC
    CCAGTCATCA TTAGTTCCTC AGCTGCACAA GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA
    AATGGAGCAG CCGGTGGTCT TGCTGGAACA ACAAATGGAC CATTTCGCCC TTTAGGAACT
    CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG CCCAGTAACA ACCTGGCATC CAGCTCTTTC
    TACGGCAACA ACTCTCTGAG CAGCAATTCA CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC
    GCCCAGCCTG CCAACACATC CTTGGGATTT GGAAGTAGCA GTTCTCTCGG CGCCACCCTG
    GGATCAGCCC TTGGAGGGTT TGGAACAGCA GTTGCAAACT CCAACACTGG CAGTGGCTCC
    CGCCGTGACT CCCTGACTGG CAGCAGTGAC CTTTATAAGA GGACATCGAG CAGCTTGACC
    CCCATTGGAC ACAGTTTTTA TAACGGCCTT AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC
    ATGCCTCTCC CTAGTCAGGG ACCAGGACAT TCACAGACAC CACCTCCTTC CCTCTCTTCA
    CATGGATCCT CTTCAAGCTT AAACCTGGGA GGACTCACAA ATGGCAGTGG AAGATACATC
    TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC
    AGCCCCAGCA GCACCCTTTT TTCCTCCTCC CGTTTGCGAT ACGGAATGTC TGATGTCATG
    CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT TTTCGAAATA ACCGATATCC TAATTTACAA
    TTGCGGGAGA TTGCTGGGCA CATAATGGAG TTTTCCCAAG ACCAGCATGG TTCCAGATTC
    ATTCAGCTGA AACTTGAGCG TGCCACACCA GCCGAACGCC AACTTGTCTT CAATGAAATC
    CTTCAGGCCG CTTACCAGCT GATGGTGGAT GTATTTGGAA ATTACGTCAT TCAGAAGTTC
    TTTGAATTTG GCAGCCTTGA ACAGAAGCTG GCTTTGGCTG AGCGGATTCG AGGCCACGTC
    CTGTCATTGG CACTGCAGAT GTATGGCTGC CGTGTTATCC AGAAAGCTCT TGAGTTTATT
    CCCTCAGACC AGCAGGTAAT TAATGAAATG GTTCGGGAAC TGGATGGCCA CGTCCTCAAG
    TGTGTGAAAG ACCAGAATGG CAATCACGTG GTGCAGAAGT GCATCGAATG TGTGCAGCCC
    CAGTCTCTGC AGTTCATCAT CGATGCGTTT AAGGGGCAGG TGTTTGCTTT GTCCACGCAT
    CCTTATGGCT GCCGAGTGAT TCAGAGGATT CTGGAGCACT GTCTCCCTGA CCAGACACTC
    CCTATTTTAG AGGAGCTTCA CCAGCACACA GAGCAACTTG TACAGGATCA GTATGGAAAT
    TACGTAATCC AGCATGTATT GGAGCATGGT CGTCCTGAGG ATAAAAGTAA AATTGTAGCA
    GAAATTCGAG GCAATGTACT TGTGTTGAGT CAGCACAAAT TTGCAAGCAA TGTTGTGGAG
    AAGTGTGTCA CTCACGCCTC CCGTACAGAG CGCGCTGTGC TTATTGACGA GGTGTGCACC
    ATGAACGATG GTCCCCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAACTAC
    GTGGTCCAGA AGATGATCGA TGTGGCAGAG CCAGCCCAGA GGAAGATCGT CATGCACAAG
    ATCCGGCCCC ACATCGCGAC TCTTCGCAAG TATACTTACG GCAAGCACAT CCTGGCCAAG
    CTGGAGAAAT ACTACATGAA GAATGGCGTT GACTTAGGGC CCATCTGCGG CCCCCCTAAT
    GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831758.1
    CDS134..3265
    Translation

    Target ORF information:

    RefSeq Version XM_024975990.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGCAGGTT TAGCTCCCGC TGCATTCGTC
    CCCAACCCAT ATATCATCAG TGCTGCGCCC CCAGGGACGG ACCCCTACAC AGCTGGATTG
    GCTGCAGCAG CGACGCTAGG CCCGGCTGTG GTCCCTCCCC AGTATTACGG AGTTACTCCC
    TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG CAGCAAGCCG CCGCCGCTGC TGCAGCAACA
    AATTCTGCTA ATCAGCAGAC CACCCCACAG GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT
    GGAGGAGCCA GCCAGCGTCC TTTGACGCCG AATCAGAACC AGCAGGGACA GCAAGCCGAT
    CCACTGGTGG CAGCTGCAGC GGTGAATTCT GCCCTTGCGT TTGGACAAGG ACTGGCTGCG
    GGCATGCCAG GTTATCCAGT CTTGGCTCCT GCTGCTTACT ATGACCAAAC CGGTGCCCTT
    GTGGTGAATG CAGGAGCGAG AAATGGTCTT GGAGCTCCTG TTCGACTTGT AGCTCCCGCC
    CCAGTCATCA TTAGTTCCTC AGCTGCACAA GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA
    AATGGAGCAG CCGGTGGTCT TGCTGGAACA ACAAATGGAC CATTTCGCCC TTTAGGAACT
    CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG CCCAGTAACA ACCTGGCATC CAGCTCTTTC
    TACGGCAACA ACTCTCTGAG CAGCAATTCA CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC
    GCCCAGCCTG CCAACACATC CTTGGGATTT GGAAGTAGCA GTTCTCTCGG CGCCACCCTG
    GGATCAGCCC TTGGAGGGTT TGGAACAGCA GTTGCAAACT CCAACACTGG CAGTGGCTCC
    CGCCGTGACT CCCTGACTGG CAGCAGTGAC CTTTATAAGA GGACATCGAG CAGCTTGACC
    CCCATTGGAC ACAGTTTTTA TAACGGCCTT AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC
    ATGCCTCTCC CTAGTCAGGG ACCAGGACAT TCACAGACAC CACCTCCTTC CCTCTCTTCA
    CATGGATCCT CTTCAAGCTT AAACCTGGGA GGACTCACAA ATGGCAGTGG AAGATACATC
    TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC
    AGCCCCAGCA GCACCCTTTT TTCCTCCTCC CGTTTGCGAT ACGGAATGTC TGATGTCATG
    CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT TTTCGAAATA ACCGATATCC TAATTTACAA
    TTGCGGGAGA TTGCTGGGCA CATAATGGAG TTTTCCCAAG ACCAGCATGG TTCCAGATTC
    ATTCAGCTGA AACTTGAGCG TGCCACACCA GCCGAACGCC AACTTGTCTT CAATGAAATC
    CTTCAGGCCG CTTACCAGCT GATGGTGGAT GTATTTGGAA ATTACGTCAT TCAGAAGTTC
    TTTGAATTTG GCAGCCTTGA ACAGAAGCTG GCTTTGGCTG AGCGGATTCG AGGCCACGTC
    CTGTCATTGG CACTGCAGAT GTATGGCTGC CGTGTTATCC AGAAAGCTCT TGAGTTTATT
    CCCTCAGACC AGCAGGTAAT TAATGAAATG GTTCGGGAAC TGGATGGCCA CGTCCTCAAG
    TGTGTGAAAG ACCAGAATGG CAATCACGTG GTGCAGAAGT GCATCGAATG TGTGCAGCCC
    CAGTCTCTGC AGTTCATCAT CGATGCGTTT AAGGGGCAGG TGTTTGCTTT GTCCACGCAT
    CCTTATGGCT GCCGAGTGAT TCAGAGGATT CTGGAGCACT GTCTCCCTGA CCAGACACTC
    CCTATTTTAG AGGAGCTTCA CCAGCACACA GAGCAACTTG TACAGGATCA GTATGGAAAT
    TACGTAATCC AGCATGTATT GGAGCATGGT CGTCCTGAGG ATAAAAGTAA AATTGTAGCA
    GAAATTCGAG GCAATGTACT TGTGTTGAGT CAGCACAAAT TTGCAAGCAA TGTTGTGGAG
    AAGTGTGTCA CTCACGCCTC CCGTACAGAG CGCGCTGTGC TTATTGACGA GGTGTGCACC
    ATGAACGATG GTCCCCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAACTAC
    GTGGTCCAGA AGATGATCGA TGTGGCAGAG CCAGCCCAGA GGAAGATCGT CATGCACAAG
    ATCCGGCCCC ACATCGCGAC TCTTCGCAAG TATACTTACG GCAAGCACAT CCTGGCCAAG
    CTGGAGAAAT ACTACATGAA GAATGGCGTT GACTTAGGGC CCATCTGCGG CCCCCCTAAT
    GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227444
    Clone ID Related Accession (Same CDS sequence) XM_024975997.1
    Accession Version XM_024975997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC
    AACCCATATA TCATCAGTGC TGCGCCCCCA GGGACGGACC CCTACACAGC TGGATTGGCT
    GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC CCTCCCCAGT ATTACGGAGT TACTCCCTGG
    GGAGTCTACC CTGCCAGTCT TTTCCAGCAG CAAGCCGCCG CCGCTGCTGC AGCAACAAAT
    TCTGCTAATC AGCAGACCAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACGCCGAAT CAGAACCAGC AGGGACAGCA AGCCGATCCA
    CTGGTGGCAG CTGCAGCGGT GAATTCTGCC CTTGCGTTTG GACAAGGACT GGCTGCGGGC
    ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG TGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGA GCTCCTGTTC GACTTGTAGC TCCCGCCCCA
    GTCATCATTA GTTCCTCAGC TGCACAAGCA GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACTCAG
    CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAACC TGGCATCCAG CTCTTTCTAC
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGA CTCACAAATG GCAGTGGAAG ATACATCTCT
    GCTGCTCCAG GCGCTGAAGC CAAGTACCGC AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT TTGCGATACG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTTT GGAAGATTTT CGAAATAACC GATATCCTAA TTTACAATTG
    CGGGAGATTG CTGGGCACAT AATGGAGTTT TCCCAAGACC AGCATGGTTC CAGATTCATT
    CAGCTGAAAC TTGAGCGTGC CACACCAGCC GAACGCCAAC TTGTCTTCAA TGAAATCCTT
    CAGGCCGCTT ACCAGCTGAT GGTGGATGTA TTTGGAAATT ACGTCATTCA GAAGTTCTTT
    GAATTTGGCA GCCTTGAACA GAAGCTGGCT TTGGCTGAGC GGATTCGAGG CCACGTCCTG
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA GTTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAAATGGTT CGGGAACTGG ATGGCCACGT CCTCAAGTGT
    GTGAAAGACC AGAATGGCAA TCACGTGGTG CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTCTGCAGT TCATCATCGA TGCGTTTAAG GGGCAGGTGT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATTCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAC
    GTAATCCAGC ATGTATTGGA GCATGGTCGT CCTGAGGATA AAAGTAAAAT TGTAGCAGAA
    ATTCGAGGCA ATGTACTTGT GTTGAGTCAG CACAAATTTG CAAGCAATGT TGTGGAGAAG
    TGTGTCACTC ACGCCTCCCG TACAGAGCGC GCTGTGCTTA TTGACGAGGT GTGCACCATG
    AACGATGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGAGGA AGATCGTCAT GCACAAGATC
    CGGCCCCACA TCGCGACTCT TCGCAAGTAT ACTTACGGCA AGCACATCCT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGCGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831765.1
    CDS134..3262
    Translation

    Target ORF information:

    RefSeq Version XM_024975997.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    CTGTTCCAAA GACCCAATGC GCTTGCTGTC CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG
    TATGCGTTAG CAGCCGCGCA TCAACCCCAC ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC
    AACCCATATA TCATCAGTGC TGCGCCCCCA GGGACGGACC CCTACACAGC TGGATTGGCT
    GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC CCTCCCCAGT ATTACGGAGT TACTCCCTGG
    GGAGTCTACC CTGCCAGTCT TTTCCAGCAG CAAGCCGCCG CCGCTGCTGC AGCAACAAAT
    TCTGCTAATC AGCAGACCAC CCCACAGGCT CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA
    GGAGCCAGCC AGCGTCCTTT GACGCCGAAT CAGAACCAGC AGGGACAGCA AGCCGATCCA
    CTGGTGGCAG CTGCAGCGGT GAATTCTGCC CTTGCGTTTG GACAAGGACT GGCTGCGGGC
    ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT GCTTACTATG ACCAAACCGG TGCCCTTGTG
    GTGAATGCAG GAGCGAGAAA TGGTCTTGGA GCTCCTGTTC GACTTGTAGC TCCCGCCCCA
    GTCATCATTA GTTCCTCAGC TGCACAAGCA GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT
    GGAGCAGCCG GTGGTCTTGC TGGAACAACA AATGGACCAT TTCGCCCTTT AGGAACTCAG
    CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC AGTAACAACC TGGCATCCAG CTCTTTCTAC
    GGCAACAACT CTCTGAGCAG CAATTCACAG AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC
    CAGCCTGCCA ACACATCCTT GGGATTTGGA AGTAGCAGTT CTCTCGGCGC CACCCTGGGA
    TCAGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA ACACTGGCAG TGGCTCCCGC
    CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA CATCGAGCAG CTTGACCCCC
    ATTGGACACA GTTTTTATAA CGGCCTTAGC TTTTCCTCCT CTCCTGGACC CGTGGGCATG
    CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC CTCCTTCCCT CTCTTCACAT
    GGATCCTCTT CAAGCTTAAA CCTGGGAGGA CTCACAAATG GCAGTGGAAG ATACATCTCT
    GCTGCTCCAG GCGCTGAAGC CAAGTACCGC AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC
    CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT TTGCGATACG GAATGTCTGA TGTCATGCCC
    TCTGGCAGGA GCAGGCTTTT GGAAGATTTT CGAAATAACC GATATCCTAA TTTACAATTG
    CGGGAGATTG CTGGGCACAT AATGGAGTTT TCCCAAGACC AGCATGGTTC CAGATTCATT
    CAGCTGAAAC TTGAGCGTGC CACACCAGCC GAACGCCAAC TTGTCTTCAA TGAAATCCTT
    CAGGCCGCTT ACCAGCTGAT GGTGGATGTA TTTGGAAATT ACGTCATTCA GAAGTTCTTT
    GAATTTGGCA GCCTTGAACA GAAGCTGGCT TTGGCTGAGC GGATTCGAGG CCACGTCCTG
    TCATTGGCAC TGCAGATGTA TGGCTGCCGT GTTATCCAGA AAGCTCTTGA GTTTATTCCC
    TCAGACCAGC AGGTAATTAA TGAAATGGTT CGGGAACTGG ATGGCCACGT CCTCAAGTGT
    GTGAAAGACC AGAATGGCAA TCACGTGGTG CAGAAGTGCA TCGAATGTGT GCAGCCCCAG
    TCTCTGCAGT TCATCATCGA TGCGTTTAAG GGGCAGGTGT TTGCTTTGTC CACGCATCCT
    TATGGCTGCC GAGTGATTCA GAGGATTCTG GAGCACTGTC TCCCTGACCA GACACTCCCT
    ATTTTAGAGG AGCTTCACCA GCACACAGAG CAACTTGTAC AGGATCAGTA TGGAAATTAC
    GTAATCCAGC ATGTATTGGA GCATGGTCGT CCTGAGGATA AAAGTAAAAT TGTAGCAGAA
    ATTCGAGGCA ATGTACTTGT GTTGAGTCAG CACAAATTTG CAAGCAATGT TGTGGAGAAG
    TGTGTCACTC ACGCCTCCCG TACAGAGCGC GCTGTGCTTA TTGACGAGGT GTGCACCATG
    AACGATGGTC CCCACAGTGC CTTATACACC ATGATGAAGG ACCAGTATGC CAACTACGTG
    GTCCAGAAGA TGATCGATGT GGCAGAGCCA GCCCAGAGGA AGATCGTCAT GCACAAGATC
    CGGCCCCACA TCGCGACTCT TCGCAAGTAT ACTTACGGCA AGCACATCCT GGCCAAGCTG
    GAGAAATACT ACATGAAGAA TGGCGTTGAC TTAGGGCCCA TCTGCGGCCC CCCTAATGGT
    ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227445
    Clone ID Related Accession (Same CDS sequence) XM_024976002.1
    Accession Version XM_024976002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGACTCACA
    AATGGCAGTG GAAGATACAT CTCTGCTGCT CCAGGCGCTG AAGCCAAGTA CCGCAGCGCA
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTTT TTTCCTCCTC CCGTTTGCGA
    TACGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTTTGGAAGA TTTTCGAAAT
    AACCGATATC CTAATTTACA ATTGCGGGAG ATTGCTGGGC ACATAATGGA GTTTTCCCAA
    GACCAGCATG GTTCCAGATT CATTCAGCTG AAACTTGAGC GTGCCACACC AGCCGAACGC
    CAACTTGTCT TCAATGAAAT CCTTCAGGCC GCTTACCAGC TGATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAGAAGTT CTTTGAATTT GGCAGCCTTG AACAGAAGCT GGCTTTGGCT
    GAGCGGATTC GAGGCCACGT CCTGTCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAGTTTAT TCCCTCAGAC CAGCAGAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831770.1
    CDS119..3445
    Translation

    Target ORF information:

    RefSeq Version XM_024976002.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGG AGGACTCACA
    AATGGCAGTG GAAGATACAT CTCTGCTGCT CCAGGCGCTG AAGCCAAGTA CCGCAGCGCA
    AGCAGTGCCT CCAGCCTCTT CAGCCCCAGC AGCACCCTTT TTTCCTCCTC CCGTTTGCGA
    TACGGAATGT CTGATGTCAT GCCCTCTGGC AGGAGCAGGC TTTTGGAAGA TTTTCGAAAT
    AACCGATATC CTAATTTACA ATTGCGGGAG ATTGCTGGGC ACATAATGGA GTTTTCCCAA
    GACCAGCATG GTTCCAGATT CATTCAGCTG AAACTTGAGC GTGCCACACC AGCCGAACGC
    CAACTTGTCT TCAATGAAAT CCTTCAGGCC GCTTACCAGC TGATGGTGGA TGTATTTGGA
    AATTACGTCA TTCAGAAGTT CTTTGAATTT GGCAGCCTTG AACAGAAGCT GGCTTTGGCT
    GAGCGGATTC GAGGCCACGT CCTGTCATTG GCACTGCAGA TGTATGGCTG CCGTGTTATC
    CAGAAAGCTC TTGAGTTTAT TCCCTCAGAC CAGCAGAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227446
    Clone ID Related Accession (Same CDS sequence) XM_024976005.1
    Accession Version XM_024976005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831773.1
    CDS119..3442
    Translation

    Target ORF information:

    RefSeq Version XM_024976005.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGAATGAAA TGGTTCGGGA ACTGGATGGC
    CACGTCCTCA AGTGTGTGAA AGACCAGAAT GGCAATCACG TGGTGCAGAA GTGCATCGAA
    TGTGTGCAGC CCCAGTCTCT GCAGTTCATC ATCGATGCGT TTAAGGGGCA GGTGTTTGCT
    TTGTCCACGC ATCCTTATGG CTGCCGAGTG ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT
    GACCAGACAC TCCCTATTTT AGAGGAGCTT CACCAGCACA CAGAGCAACT TGTACAGGAT
    CAGTATGGAA ATTACGTAAT CCAGCATGTA TTGGAGCATG GTCGTCCTGA GGATAAAAGT
    AAAATTGTAG CAGAAATTCG AGGCAATGTA CTTGTGTTGA GTCAGCACAA ATTTGCAAGC
    AATGTTGTGG AGAAGTGTGT CACTCACGCC TCCCGTACAG AGCGCGCTGT GCTTATTGAC
    GAGGTGTGCA CCATGAACGA TGGTCCCCAC AGTGCCTTAT ACACCATGAT GAAGGACCAG
    TATGCCAACT ACGTGGTCCA GAAGATGATC GATGTGGCAG AGCCAGCCCA GAGGAAGATC
    GTCATGCACA AGATCCGGCC CCACATCGCG ACTCTTCGCA AGTATACTTA CGGCAAGCAC
    ATCCTGGCCA AGCTGGAGAA ATACTACATG AAGAATGGCG TTGACTTAGG GCCCATCTGC
    GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227447
    Clone ID Related Accession (Same CDS sequence) XM_024976007.1
    Accession Version XM_024976007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGAATGAAAT GGTTCGGGAA
    CTGGATGGCC ACGTCCTCAA GTGTGTGAAA GACCAGAATG GCAATCACGT GGTGCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTCTG CAGTTCATCA TCGATGCGTT TAAGGGGCAG
    GTGTTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT TCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTACGTAATC CAGCATGTAT TGGAGCATGG TCGTCCTGAG
    GATAAAAGTA AAATTGTAGC AGAAATTCGA GGCAATGTAC TTGTGTTGAG TCAGCACAAA
    TTTGCAAGCA ATGTTGTGGA GAAGTGTGTC ACTCACGCCT CCCGTACAGA GCGCGCTGTG
    CTTATTGACG AGGTGTGCAC CATGAACGAT GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    AGGAAGATCG TCATGCACAA GATCCGGCCC CACATCGCGA CTCTTCGCAA GTATACTTAC
    GGCAAGCACA TCCTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGCG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831775.1
    CDS119..3391
    Translation

    Target ORF information:

    RefSeq Version XM_024976007.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGAATGAAAT GGTTCGGGAA
    CTGGATGGCC ACGTCCTCAA GTGTGTGAAA GACCAGAATG GCAATCACGT GGTGCAGAAG
    TGCATCGAAT GTGTGCAGCC CCAGTCTCTG CAGTTCATCA TCGATGCGTT TAAGGGGCAG
    GTGTTTGCTT TGTCCACGCA TCCTTATGGC TGCCGAGTGA TTCAGAGGAT TCTGGAGCAC
    TGTCTCCCTG ACCAGACACT CCCTATTTTA GAGGAGCTTC ACCAGCACAC AGAGCAACTT
    GTACAGGATC AGTATGGAAA TTACGTAATC CAGCATGTAT TGGAGCATGG TCGTCCTGAG
    GATAAAAGTA AAATTGTAGC AGAAATTCGA GGCAATGTAC TTGTGTTGAG TCAGCACAAA
    TTTGCAAGCA ATGTTGTGGA GAAGTGTGTC ACTCACGCCT CCCGTACAGA GCGCGCTGTG
    CTTATTGACG AGGTGTGCAC CATGAACGAT GGTCCCCACA GTGCCTTATA CACCATGATG
    AAGGACCAGT ATGCCAACTA CGTGGTCCAG AAGATGATCG ATGTGGCAGA GCCAGCCCAG
    AGGAAGATCG TCATGCACAA GATCCGGCCC CACATCGCGA CTCTTCGCAA GTATACTTAC
    GGCAAGCACA TCCTGGCCAA GCTGGAGAAA TACTACATGA AGAATGGCGT TGACTTAGGG
    CCCATCTGCG GCCCCCCTAA TGGTATTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38270
    Clone ID Related Accession (Same CDS sequence) NM_001193123.1
    Accession Version NM_001193123.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product pumilio homolog 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_868856.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571255.26491.1, SRR5571265.24806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180052.1
    CDS99..3659
    Translation

    Target ORF information:

    RefSeq Version NM_001193123.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16796
    Clone ID Related Accession (Same CDS sequence) XM_005202942.4 , XM_005202941.4 , XM_005202941.3 , XM_005202942.3
    Accession Version XM_005202942.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202942.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202999.1
    CDS148..3729
    Translation

    Target ORF information:

    RefSeq Version XM_005202942.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202942.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16796
    Clone ID Related Accession (Same CDS sequence) XM_005202942.4 , XM_005202941.4 , XM_005202941.3 , XM_005202942.3
    Accession Version XM_005202941.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202941.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202998.1
    CDS134..3715
    Translation

    Target ORF information:

    RefSeq Version XM_005202941.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202941.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16796
    Clone ID Related Accession (Same CDS sequence) XM_005202942.4 , XM_005202941.4 , XM_005202941.3 , XM_005202942.3
    Accession Version XM_005202941.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202998.1
    CDS118..3699
    Translation

    Target ORF information:

    RefSeq Version XM_005202941.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202941.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16796
    Clone ID Related Accession (Same CDS sequence) XM_005202942.4 , XM_005202941.4 , XM_005202941.3 , XM_005202942.3
    Accession Version XM_005202942.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202999.1
    CDS75..3656
    Translation

    Target ORF information:

    RefSeq Version XM_005202942.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202942.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGGTAAT TAATGAAATG
    GTTCGGGAAC TGGATGGCCA CGTCCTCAAG TGTGTGAAAG ACCAGAATGG CAATCACGTG
    GTGCAGAAGT GCATCGAATG TGTGCAGCCC CAGTCTCTGC AGTTCATCAT CGATGCGTTT
    AAGGGGCAGG TGTTTGCTTT GTCCACGCAT CCTTATGGCT GCCGAGTGAT TCAGAGGATT
    CTGGAGCACT GTCTCCCTGA CCAGACACTC CCTATTTTAG AGGAGCTTCA CCAGCACACA
    GAGCAACTTG TACAGGATCA GTATGGAAAT TACGTAATCC AGCATGTATT GGAGCATGGT
    CGTCCTGAGG ATAAAAGTAA AATTGTAGCA GAAATTCGAG GCAATGTACT TGTGTTGAGT
    CAGCACAAAT TTGCAAGCAA TGTTGTGGAG AAGTGTGTCA CTCACGCCTC CCGTACAGAG
    CGCGCTGTGC TTATTGACGA GGTGTGCACC ATGAACGATG GTCCCCACAG TGCCTTATAC
    ACCATGATGA AGGACCAGTA TGCCAACTAC GTGGTCCAGA AGATGATCGA TGTGGCAGAG
    CCAGCCCAGA GGAAGATCGT CATGCACAAG ATCCGGCCCC ACATCGCGAC TCTTCGCAAG
    TATACTTACG GCAAGCACAT CCTGGCCAAG CTGGAGAAAT ACTACATGAA GAATGGCGTT
    GACTTAGGGC CCATCTGCGG CCCCCCTAAT GGTATTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16797
    Clone ID Related Accession (Same CDS sequence) XM_005202943.4 , XM_005202943.3
    Accession Version XM_005202943.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202943.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203000.1
    CDS134..3712
    Translation

    Target ORF information:

    RefSeq Version XM_005202943.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202943.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16797
    Clone ID Related Accession (Same CDS sequence) XM_005202943.4 , XM_005202943.3
    Accession Version XM_005202943.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203000.1
    CDS118..3696
    Translation

    Target ORF information:

    RefSeq Version XM_005202943.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202943.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCTCTTGCC AGGTAAAAAG TTTTGGGAAA CAGATGAATC CAGCAAAGAT
    GGACCAAAAG GAATATTCCT GGGTGATCAA TGGCGAGACA GTGCCTGGGG AACATCAGAT
    CATTCAGTTT CCCAGCCAAT CATGGTGCAG AGAAGACCTG GTCAGAGTTT CCATGTGAAC
    AGTGAGGTCA ATTCTGTACT GTCCCCACGG TCGGAGAGTG GGGGACTAGG CGTTAGCATG
    GTGGAGTATG TATTGAGCTC ATCCCCGGGC GATTCCTGTC TAAGAAAAGG AGGATTTGGC
    CCAAGGGATG CAGACAGTGA TGAAAACGAC AAAGGTGAAA AGAAGAACAA GGGTACGTTT
    GATGGAGATA AGCTAGGAGA TTTGAAGGAG GAGGGTGATG TGATGGACAA GACCAATGGT
    TTACCAGTGC AGAATGGGAT TGACGCAGAC GTCAAAGATT TTAGCCGTAC CCCTGGTAAT
    TGCCAGAACT CTGCTAATGA AGTGGATCTT CTGGGTCCAA ACCAGAATGG TTCTGAGGGT
    TTAGCCCAGC TGACCAGCAC CAATGGTGCC AAGCCTGTGG AGGATTTCTC CAACATGGAG
    TCCCAGAGTG TCCCCTTGGA CCCCATGGAA CATGTGGGCA TGGAGCCTCT TCAGTTTGAT
    TATTCAGGCA CGCAGGTACC TGTGGACTCA GCAGCAGCAA CTGTGGGACT TTTTGACTAC
    AATTCTCAAC AACAGCTGTT CCAAAGACCC AATGCGCTTG CTGTCCAGCA GTTGACAGCC
    GCTCAGCAGC AGCAGTATGC GTTAGCAGCC GCGCATCAAC CCCACATCGG TATGTTTTCA
    GCAGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16798
    Clone ID Related Accession (Same CDS sequence) XM_005202944.3 , XM_005202944.4
    Accession Version XM_005202944.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203001.1
    CDS118..3693
    Translation

    Target ORF information:

    RefSeq Version XM_005202944.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202944.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16798
    Clone ID Related Accession (Same CDS sequence) XM_005202944.3 , XM_005202944.4
    Accession Version XM_005202944.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202944.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203001.1
    CDS134..3709
    Translation

    Target ORF information:

    RefSeq Version XM_005202944.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202944.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GTTGCAAACT CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC
    CTTTATAAGA GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT
    AGCTTTTCCT CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT
    TCACAGACAC CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA
    GGACTCACAA ATGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC
    CGCAGCGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACCCTTTT TTCCTCCTCC
    CGTTTGCGAT ACGGAATGTC TGATGTCATG CCCTCTGGCA GGAGCAGGCT TTTGGAAGAT
    TTTCGAAATA ACCGATATCC TAATTTACAA TTGCGGGAGA TTGCTGGGCA CATAATGGAG
    TTTTCCCAAG ACCAGCATGG TTCCAGATTC ATTCAGCTGA AACTTGAGCG TGCCACACCA
    GCCGAACGCC AACTTGTCTT CAATGAAATC CTTCAGGCCG CTTACCAGCT GATGGTGGAT
    GTATTTGGAA ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGCCTTGA ACAGAAGCTG
    GCTTTGGCTG AGCGGATTCG AGGCCACGTC CTGTCATTGG CACTGCAGAT GTATGGCTGC
    CGTGTTATCC AGAAAGCTCT TGAGTTTATT CCCTCAGACC AGCAGAATGA AATGGTTCGG
    GAACTGGATG GCCACGTCCT CAAGTGTGTG AAAGACCAGA ATGGCAATCA CGTGGTGCAG
    AAGTGCATCG AATGTGTGCA GCCCCAGTCT CTGCAGTTCA TCATCGATGC GTTTAAGGGG
    CAGGTGTTTG CTTTGTCCAC GCATCCTTAT GGCTGCCGAG TGATTCAGAG GATTCTGGAG
    CACTGTCTCC CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAA
    CTTGTACAGG ATCAGTATGG AAATTACGTA ATCCAGCATG TATTGGAGCA TGGTCGTCCT
    GAGGATAAAA GTAAAATTGT AGCAGAAATT CGAGGCAATG TACTTGTGTT GAGTCAGCAC
    AAATTTGCAA GCAATGTTGT GGAGAAGTGT GTCACTCACG CCTCCCGTAC AGAGCGCGCT
    GTGCTTATTG ACGAGGTGTG CACCATGAAC GATGGTCCCC ACAGTGCCTT ATACACCATG
    ATGAAGGACC AGTATGCCAA CTACGTGGTC CAGAAGATGA TCGATGTGGC AGAGCCAGCC
    CAGAGGAAGA TCGTCATGCA CAAGATCCGG CCCCACATCG CGACTCTTCG CAAGTATACT
    TACGGCAAGC ACATCCTGGC CAAGCTGGAG AAATACTACA TGAAGAATGG CGTTGACTTA
    GGGCCCATCT GCGGCCCCCC TAATGGTATT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16799
    Clone ID Related Accession (Same CDS sequence) XM_005202945.4 , XM_005202945.3
    Accession Version XM_005202945.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202945.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGGTAATTA ATGAAATGGT TCGGGAACTG
    GATGGCCACG TCCTCAAGTG TGTGAAAGAC CAGAATGGCA ATCACGTGGT GCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTCTGCAG TTCATCATCG ATGCGTTTAA GGGGCAGGTG
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATTCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA CGTAATCCAG CATGTATTGG AGCATGGTCG TCCTGAGGAT
    AAAAGTAAAA TTGTAGCAGA AATTCGAGGC AATGTACTTG TGTTGAGTCA GCACAAATTT
    GCAAGCAATG TTGTGGAGAA GTGTGTCACT CACGCCTCCC GTACAGAGCG CGCTGTGCTT
    ATTGACGAGG TGTGCACCAT GAACGATGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGAGG
    AAGATCGTCA TGCACAAGAT CCGGCCCCAC ATCGCGACTC TTCGCAAGTA TACTTACGGC
    AAGCACATCC TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGCGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203002.1
    CDS134..3703
    Translation

    Target ORF information:

    RefSeq Version XM_005202945.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202945.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGGTAATTA ATGAAATGGT TCGGGAACTG
    GATGGCCACG TCCTCAAGTG TGTGAAAGAC CAGAATGGCA ATCACGTGGT GCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTCTGCAG TTCATCATCG ATGCGTTTAA GGGGCAGGTG
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATTCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA CGTAATCCAG CATGTATTGG AGCATGGTCG TCCTGAGGAT
    AAAAGTAAAA TTGTAGCAGA AATTCGAGGC AATGTACTTG TGTTGAGTCA GCACAAATTT
    GCAAGCAATG TTGTGGAGAA GTGTGTCACT CACGCCTCCC GTACAGAGCG CGCTGTGCTT
    ATTGACGAGG TGTGCACCAT GAACGATGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGAGG
    AAGATCGTCA TGCACAAGAT CCGGCCCCAC ATCGCGACTC TTCGCAAGTA TACTTACGGC
    AAGCACATCC TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGCGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16799
    Clone ID Related Accession (Same CDS sequence) XM_005202945.4 , XM_005202945.3
    Accession Version XM_005202945.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGGTAATTA ATGAAATGGT TCGGGAACTG
    GATGGCCACG TCCTCAAGTG TGTGAAAGAC CAGAATGGCA ATCACGTGGT GCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTCTGCAG TTCATCATCG ATGCGTTTAA GGGGCAGGTG
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATTCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA CGTAATCCAG CATGTATTGG AGCATGGTCG TCCTGAGGAT
    AAAAGTAAAA TTGTAGCAGA AATTCGAGGC AATGTACTTG TGTTGAGTCA GCACAAATTT
    GCAAGCAATG TTGTGGAGAA GTGTGTCACT CACGCCTCCC GTACAGAGCG CGCTGTGCTT
    ATTGACGAGG TGTGCACCAT GAACGATGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGAGG
    AAGATCGTCA TGCACAAGAT CCGGCCCCAC ATCGCGACTC TTCGCAAGTA TACTTACGGC
    AAGCACATCC TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGCGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203002.1
    CDS118..3687
    Translation

    Target ORF information:

    RefSeq Version XM_005202945.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGCAGGTTTA
    GCTCCCGCTG CATTCGTCCC CAACCCATAT ATCATCAGTG CTGCGCCCCC AGGGACGGAC
    CCCTACACAG CTGGATTGGC TGCAGCAGCG ACGCTAGGCC CGGCTGTGGT CCCTCCCCAG
    TATTACGGAG TTACTCCCTG GGGAGTCTAC CCTGCCAGTC TTTTCCAGCA GCAAGCCGCC
    GCCGCTGCTG CAGCAACAAA TTCTGCTAAT CAGCAGACCA CCCCACAGGC TCAGCAAGGG
    CAGCAGCAGG TTCTCCGTGG AGGAGCCAGC CAGCGTCCTT TGACGCCGAA TCAGAACCAG
    CAGGGACAGC AAGCCGATCC ACTGGTGGCA GCTGCAGCGG TGAATTCTGC CCTTGCGTTT
    GGACAAGGAC TGGCTGCGGG CATGCCAGGT TATCCAGTCT TGGCTCCTGC TGCTTACTAT
    GACCAAACCG GTGCCCTTGT GGTGAATGCA GGAGCGAGAA ATGGTCTTGG AGCTCCTGTT
    CGACTTGTAG CTCCCGCCCC AGTCATCATT AGTTCCTCAG CTGCACAAGC AGCTGTTGCA
    GCGGCCGCAG CTTCAGCAAA TGGAGCAGCC GGTGGTCTTG CTGGAACAAC AAATGGACCA
    TTTCGCCCTT TAGGAACTCA GCAGCCTCAG CCCCAGCCCC AGCAGCAGCC CAGTAACAAC
    CTGGCATCCA GCTCTTTCTA CGGCAACAAC TCTCTGAGCA GCAATTCACA GAGCAGCTCC
    CTCTTCTCCC AGGGCTCCGC CCAGCCTGCC AACACATCCT TGGGATTTGG AAGTAGCAGT
    TCTCTCGGCG CCACCCTGGG ATCAGCCCTT GGAGGGTTTG GAACAGCAGT TGCAAACTCC
    AACACTGGCA GTGGCTCCCG CCGTGACTCC CTGACTGGCA GCAGTGACCT TTATAAGAGG
    ACATCGAGCA GCTTGACCCC CATTGGACAC AGTTTTTATA ACGGCCTTAG CTTTTCCTCC
    TCTCCTGGAC CCGTGGGCAT GCCTCTCCCT AGTCAGGGAC CAGGACATTC ACAGACACCA
    CCTCCTTCCC TCTCTTCACA TGGATCCTCT TCAAGCTTAA ACCTGGGAGG ACTCACAAAT
    GGCAGTGGAA GATACATCTC TGCTGCTCCA GGCGCTGAAG CCAAGTACCG CAGCGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACCCTTTTTT CCTCCTCCCG TTTGCGATAC
    GGAATGTCTG ATGTCATGCC CTCTGGCAGG AGCAGGCTTT TGGAAGATTT TCGAAATAAC
    CGATATCCTA ATTTACAATT GCGGGAGATT GCTGGGCACA TAATGGAGTT TTCCCAAGAC
    CAGCATGGTT CCAGATTCAT TCAGCTGAAA CTTGAGCGTG CCACACCAGC CGAACGCCAA
    CTTGTCTTCA ATGAAATCCT TCAGGCCGCT TACCAGCTGA TGGTGGATGT ATTTGGAAAT
    TACGTCATTC AGAAGTTCTT TGAATTTGGC AGCCTTGAAC AGAAGCTGGC TTTGGCTGAG
    CGGATTCGAG GCCACGTCCT GTCATTGGCA CTGCAGATGT ATGGCTGCCG TGTTATCCAG
    AAAGCTCTTG AGTTTATTCC CTCAGACCAG CAGGTAATTA ATGAAATGGT TCGGGAACTG
    GATGGCCACG TCCTCAAGTG TGTGAAAGAC CAGAATGGCA ATCACGTGGT GCAGAAGTGC
    ATCGAATGTG TGCAGCCCCA GTCTCTGCAG TTCATCATCG ATGCGTTTAA GGGGCAGGTG
    TTTGCTTTGT CCACGCATCC TTATGGCTGC CGAGTGATTC AGAGGATTCT GGAGCACTGT
    CTCCCTGACC AGACACTCCC TATTTTAGAG GAGCTTCACC AGCACACAGA GCAACTTGTA
    CAGGATCAGT ATGGAAATTA CGTAATCCAG CATGTATTGG AGCATGGTCG TCCTGAGGAT
    AAAAGTAAAA TTGTAGCAGA AATTCGAGGC AATGTACTTG TGTTGAGTCA GCACAAATTT
    GCAAGCAATG TTGTGGAGAA GTGTGTCACT CACGCCTCCC GTACAGAGCG CGCTGTGCTT
    ATTGACGAGG TGTGCACCAT GAACGATGGT CCCCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAACTACGT GGTCCAGAAG ATGATCGATG TGGCAGAGCC AGCCCAGAGG
    AAGATCGTCA TGCACAAGAT CCGGCCCCAC ATCGCGACTC TTCGCAAGTA TACTTACGGC
    AAGCACATCC TGGCCAAGCT GGAGAAATAC TACATGAAGA ATGGCGTTGA CTTAGGGCCC
    ATCTGCGGCC CCCCTAATGG TATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16800
    Clone ID Related Accession (Same CDS sequence) XM_005202946.3 , XM_005202946.4
    Accession Version XM_005202946.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG GTAATTAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203003.1
    CDS118..3684
    Translation

    Target ORF information:

    RefSeq Version XM_005202946.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202946.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG GTAATTAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16800
    Clone ID Related Accession (Same CDS sequence) XM_005202946.3 , XM_005202946.4
    Accession Version XM_005202946.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202946.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG GTAATTAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203003.1
    CDS134..3700
    Translation

    Target ORF information:

    RefSeq Version XM_005202946.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202946.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTTTAGCT
    CCCGCTGCAT TCGTCCCCAA CCCATATATC ATCAGTGCTG CGCCCCCAGG GACGGACCCC
    TACACAGCTG GATTGGCTGC AGCAGCGACG CTAGGCCCGG CTGTGGTCCC TCCCCAGTAT
    TACGGAGTTA CTCCCTGGGG AGTCTACCCT GCCAGTCTTT TCCAGCAGCA AGCCGCCGCC
    GCTGCTGCAG CAACAAATTC TGCTAATCAG CAGACCACCC CACAGGCTCA GCAAGGGCAG
    CAGCAGGTTC TCCGTGGAGG AGCCAGCCAG CGTCCTTTGA CGCCGAATCA GAACCAGCAG
    GGACAGCAAG CCGATCCACT GGTGGCAGCT GCAGCGGTGA ATTCTGCCCT TGCGTTTGGA
    CAAGGACTGG CTGCGGGCAT GCCAGGTTAT CCAGTCTTGG CTCCTGCTGC TTACTATGAC
    CAAACCGGTG CCCTTGTGGT GAATGCAGGA GCGAGAAATG GTCTTGGAGC TCCTGTTCGA
    CTTGTAGCTC CCGCCCCAGT CATCATTAGT TCCTCAGCTG CACAAGCAGC TGTTGCAGCG
    GCCGCAGCTT CAGCAAATGG AGCAGCCGGT GGTCTTGCTG GAACAACAAA TGGACCATTT
    CGCCCTTTAG GAACTCAGCA GCCTCAGCCC CAGCCCCAGC AGCAGCCCAG TAACAACCTG
    GCATCCAGCT CTTTCTACGG CAACAACTCT CTGAGCAGCA ATTCACAGAG CAGCTCCCTC
    TTCTCCCAGG GCTCCGCCCA GCCTGCCAAC ACATCCTTGG GATTTGGAAG TAGCAGTTCT
    CTCGGCGCCA CCCTGGGATC AGCCCTTGGA GGGTTTGGAA CAGCAGTTGC AAACTCCAAC
    ACTGGCAGTG GCTCCCGCCG TGACTCCCTG ACTGGCAGCA GTGACCTTTA TAAGAGGACA
    TCGAGCAGCT TGACCCCCAT TGGACACAGT TTTTATAACG GCCTTAGCTT TTCCTCCTCT
    CCTGGACCCG TGGGCATGCC TCTCCCTAGT CAGGGACCAG GACATTCACA GACACCACCT
    CCTTCCCTCT CTTCACATGG ATCCTCTTCA AGCTTAAACC TGGGAGGACT CACAAATGGC
    AGTGGAAGAT ACATCTCTGC TGCTCCAGGC GCTGAAGCCA AGTACCGCAG CGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACC CTTTTTTCCT CCTCCCGTTT GCGATACGGA
    ATGTCTGATG TCATGCCCTC TGGCAGGAGC AGGCTTTTGG AAGATTTTCG AAATAACCGA
    TATCCTAATT TACAATTGCG GGAGATTGCT GGGCACATAA TGGAGTTTTC CCAAGACCAG
    CATGGTTCCA GATTCATTCA GCTGAAACTT GAGCGTGCCA CACCAGCCGA ACGCCAACTT
    GTCTTCAATG AAATCCTTCA GGCCGCTTAC CAGCTGATGG TGGATGTATT TGGAAATTAC
    GTCATTCAGA AGTTCTTTGA ATTTGGCAGC CTTGAACAGA AGCTGGCTTT GGCTGAGCGG
    ATTCGAGGCC ACGTCCTGTC ATTGGCACTG CAGATGTATG GCTGCCGTGT TATCCAGAAA
    GCTCTTGAGT TTATTCCCTC AGACCAGCAG GTAATTAATG AAATGGTTCG GGAACTGGAT
    GGCCACGTCC TCAAGTGTGT GAAAGACCAG AATGGCAATC ACGTGGTGCA GAAGTGCATC
    GAATGTGTGC AGCCCCAGTC TCTGCAGTTC ATCATCGATG CGTTTAAGGG GCAGGTGTTT
    GCTTTGTCCA CGCATCCTTA TGGCTGCCGA GTGATTCAGA GGATTCTGGA GCACTGTCTC
    CCTGACCAGA CACTCCCTAT TTTAGAGGAG CTTCACCAGC ACACAGAGCA ACTTGTACAG
    GATCAGTATG GAAATTACGT AATCCAGCAT GTATTGGAGC ATGGTCGTCC TGAGGATAAA
    AGTAAAATTG TAGCAGAAAT TCGAGGCAAT GTACTTGTGT TGAGTCAGCA CAAATTTGCA
    AGCAATGTTG TGGAGAAGTG TGTCACTCAC GCCTCCCGTA CAGAGCGCGC TGTGCTTATT
    GACGAGGTGT GCACCATGAA CGATGGTCCC CACAGTGCCT TATACACCAT GATGAAGGAC
    CAGTATGCCA ACTACGTGGT CCAGAAGATG ATCGATGTGG CAGAGCCAGC CCAGAGGAAG
    ATCGTCATGC ACAAGATCCG GCCCCACATC GCGACTCTTC GCAAGTATAC TTACGGCAAG
    CACATCCTGG CCAAGCTGGA GAAATACTAC ATGAAGAATG GCGTTGACTT AGGGCCCATC
    TGCGGCCCCC CTAATGGTAT TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16801
    Clone ID Related Accession (Same CDS sequence) XM_005202947.4 , XM_005202947.3
    Accession Version XM_005202947.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202947.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203004.1
    CDS134..3634
    Translation

    Target ORF information:

    RefSeq Version XM_005202947.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202947.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16801
    Clone ID Related Accession (Same CDS sequence) XM_005202947.4 , XM_005202947.3
    Accession Version XM_005202947.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203004.1
    CDS118..3618
    Translation

    Target ORF information:

    RefSeq Version XM_005202947.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202947.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGGAACTC AGCAGCCTCA GCCCCAGCCC CAGCAGCAGC CCAGTAACAA CCTGGCATCC
    AGCTCTTTCT ACGGCAACAA CTCTCTGAGC AGCAATTCAC AGAGCAGCTC CCTCTTCTCC
    CAGGGCTCCG CCCAGCCTGC CAACACATCC TTGGGATTTG GAAGTAGCAG TTCTCTCGGC
    GCCACCCTGG GATCAGCCCT TGGAGGGTTT GGAACAGCAG TTGCAAACTC CAACACTGGC
    AGTGGCTCCC GCCGTGACTC CCTGACTGGC AGCAGTGACC TTTATAAGAG GACATCGAGC
    AGCTTGACCC CCATTGGACA CAGTTTTTAT AACGGCCTTA GCTTTTCCTC CTCTCCTGGA
    CCCGTGGGCA TGCCTCTCCC TAGTCAGGGA CCAGGACATT CACAGACACC ACCTCCTTCC
    CTCTCTTCAC ATGGATCCTC TTCAAGCTTA AACCTGGGAG GACTCACAAA TGGCAGTGGA
    AGATACATCT CTGCTGCTCC AGGCGCTGAA GCCAAGTACC GCAGCGCAAG CAGTGCCTCC
    AGCCTCTTCA GCCCCAGCAG CACCCTTTTT TCCTCCTCCC GTTTGCGATA CGGAATGTCT
    GATGTCATGC CCTCTGGCAG GAGCAGGCTT TTGGAAGATT TTCGAAATAA CCGATATCCT
    AATTTACAAT TGCGGGAGAT TGCTGGGCAC ATAATGGAGT TTTCCCAAGA CCAGCATGGT
    TCCAGATTCA TTCAGCTGAA ACTTGAGCGT GCCACACCAG CCGAACGCCA ACTTGTCTTC
    AATGAAATCC TTCAGGCCGC TTACCAGCTG ATGGTGGATG TATTTGGAAA TTACGTCATT
    CAGAAGTTCT TTGAATTTGG CAGCCTTGAA CAGAAGCTGG CTTTGGCTGA GCGGATTCGA
    GGCCACGTCC TGTCATTGGC ACTGCAGATG TATGGCTGCC GTGTTATCCA GAAAGCTCTT
    GAGTTTATTC CCTCAGACCA GCAGGTAATT AATGAAATGG TTCGGGAACT GGATGGCCAC
    GTCCTCAAGT GTGTGAAAGA CCAGAATGGC AATCACGTGG TGCAGAAGTG CATCGAATGT
    GTGCAGCCCC AGTCTCTGCA GTTCATCATC GATGCGTTTA AGGGGCAGGT GTTTGCTTTG
    TCCACGCATC CTTATGGCTG CCGAGTGATT CAGAGGATTC TGGAGCACTG TCTCCCTGAC
    CAGACACTCC CTATTTTAGA GGAGCTTCAC CAGCACACAG AGCAACTTGT ACAGGATCAG
    TATGGAAATT ACGTAATCCA GCATGTATTG GAGCATGGTC GTCCTGAGGA TAAAAGTAAA
    ATTGTAGCAG AAATTCGAGG CAATGTACTT GTGTTGAGTC AGCACAAATT TGCAAGCAAT
    GTTGTGGAGA AGTGTGTCAC TCACGCCTCC CGTACAGAGC GCGCTGTGCT TATTGACGAG
    GTGTGCACCA TGAACGATGG TCCCCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAACTACG TGGTCCAGAA GATGATCGAT GTGGCAGAGC CAGCCCAGAG GAAGATCGTC
    ATGCACAAGA TCCGGCCCCA CATCGCGACT CTTCGCAAGT ATACTTACGG CAAGCACATC
    CTGGCCAAGC TGGAGAAATA CTACATGAAG AATGGCGTTG ACTTAGGGCC CATCTGCGGC
    CCCCCTAATG GTATTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16802
    Clone ID Related Accession (Same CDS sequence) XM_005202948.4 , XM_005202948.3
    Accession Version XM_005202948.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202948.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203005.1
    CDS134..3478
    Translation

    Target ORF information:

    RefSeq Version XM_005202948.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202948.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16802
    Clone ID Related Accession (Same CDS sequence) XM_005202948.4 , XM_005202948.3
    Accession Version XM_005202948.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203005.1
    CDS118..3462
    Translation

    Target ORF information:

    RefSeq Version XM_005202948.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202948.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGGT AATTAATGAA
    ATGGTTCGGG AACTGGATGG CCACGTCCTC AAGTGTGTGA AAGACCAGAA TGGCAATCAC
    GTGGTGCAGA AGTGCATCGA ATGTGTGCAG CCCCAGTCTC TGCAGTTCAT CATCGATGCG
    TTTAAGGGGC AGGTGTTTGC TTTGTCCACG CATCCTTATG GCTGCCGAGT GATTCAGAGG
    ATTCTGGAGC ACTGTCTCCC TGACCAGACA CTCCCTATTT TAGAGGAGCT TCACCAGCAC
    ACAGAGCAAC TTGTACAGGA TCAGTATGGA AATTACGTAA TCCAGCATGT ATTGGAGCAT
    GGTCGTCCTG AGGATAAAAG TAAAATTGTA GCAGAAATTC GAGGCAATGT ACTTGTGTTG
    AGTCAGCACA AATTTGCAAG CAATGTTGTG GAGAAGTGTG TCACTCACGC CTCCCGTACA
    GAGCGCGCTG TGCTTATTGA CGAGGTGTGC ACCATGAACG ATGGTCCCCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAC TACGTGGTCC AGAAGATGAT CGATGTGGCA
    GAGCCAGCCC AGAGGAAGAT CGTCATGCAC AAGATCCGGC CCCACATCGC GACTCTTCGC
    AAGTATACTT ACGGCAAGCA CATCCTGGCC AAGCTGGAGA AATACTACAT GAAGAATGGC
    GTTGACTTAG GGCCCATCTG CGGCCCCCCT AATGGTATTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16803
    Clone ID Related Accession (Same CDS sequence) XM_005202949.3 , XM_005202949.4
    Accession Version XM_005202949.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203006.1
    CDS118..3411
    Translation

    Target ORF information:

    RefSeq Version XM_005202949.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202949.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16803
    Clone ID Related Accession (Same CDS sequence) XM_005202949.3 , XM_005202949.4
    Accession Version XM_005202949.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202949.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203006.1
    CDS134..3427
    Translation

    Target ORF information:

    RefSeq Version XM_005202949.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202949.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTATGT TTTCAGCAGG TTTAGCTCCC GCTGCATTCG TCCCCAACCC ATATATCATC
    AGTGCTGCGC CCCCAGGGAC GGACCCCTAC ACAGCTGGAT TGGCTGCAGC AGCGACGCTA
    GGCCCGGCTG TGGTCCCTCC CCAGTATTAC GGAGTTACTC CCTGGGGAGT CTACCCTGCC
    AGTCTTTTCC AGCAGCAAGC CGCCGCCGCT GCTGCAGCAA CAAATTCTGC TAATCAGCAG
    ACCACCCCAC AGGCTCAGCA AGGGCAGCAG CAGGTTCTCC GTGGAGGAGC CAGCCAGCGT
    CCTTTGACGC CGAATCAGAA CCAGCAGGGA CAGCAAGCCG ATCCACTGGT GGCAGCTGCA
    GCGGTGAATT CTGCCCTTGC GTTTGGACAA GGACTGGCTG CGGGCATGCC AGGTTATCCA
    GTCTTGGCTC CTGCTGCTTA CTATGACCAA ACCGGTGCCC TTGTGGTGAA TGCAGGAGCG
    AGAAATGGTC TTGGAGCTCC TGTTCGACTT GTAGCTCCCG CCCCAGTCAT CATTAGTTCC
    TCAGCTGCAC AAGCAGCTGT TGCAGCGGCC GCAGCTTCAG CAAATGGAGC AGCCGGTGGT
    CTTGCTGGAA CAACAAATGG ACCATTTCGC CCTTTAGGAA CTCAGCAGCC TCAGCCCCAG
    CCCCAGCAGC AGCCCAGTAA CAACCTGGCA TCCAGCTCTT TCTACGGCAA CAACTCTCTG
    AGCAGCAATT CACAGAGCAG CTCCCTCTTC TCCCAGGGCT CCGCCCAGCC TGCCAACACA
    TCCTTGGGAT TTGGAAGTAG CAGTTCTCTC GGCGCCACCC TGGGATCAGC CCTTGGAGGG
    TTTGGAACAG CAGTTGCAAA CTCCAACACT GGCAGTGGCT CCCGCCGTGA CTCCCTGACT
    GGCAGCAGTG ACCTTTATAA GAGGACATCG AGCAGCTTGA CCCCCATTGG ACACAGTTTT
    TATAACGGCC TTAGCTTTTC CTCCTCTCCT GGACCCGTGG GCATGCCTCT CCCTAGTCAG
    GGACCAGGAC ATTCACAGAC ACCACCTCCT TCCCTCTCTT CACATGGATC CTCTTCAAGC
    TTAAACCTGG GAGGACTCAC AAATGGCAGT GGAAGATACA TCTCTGCTGC TCCAGGCGCT
    GAAGCCAAGT ACCGCAGCGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACCCTT
    TTTTCCTCCT CCCGTTTGCG ATACGGAATG TCTGATGTCA TGCCCTCTGG CAGGAGCAGG
    CTTTTGGAAG ATTTTCGAAA TAACCGATAT CCTAATTTAC AATTGCGGGA GATTGCTGGG
    CACATAATGG AGTTTTCCCA AGACCAGCAT GGTTCCAGAT TCATTCAGCT GAAACTTGAG
    CGTGCCACAC CAGCCGAACG CCAACTTGTC TTCAATGAAA TCCTTCAGGC CGCTTACCAG
    CTGATGGTGG ATGTATTTGG AAATTACGTC ATTCAGAAGT TCTTTGAATT TGGCAGCCTT
    GAACAGAAGC TGGCTTTGGC TGAGCGGATT CGAGGCCACG TCCTGTCATT GGCACTGCAG
    ATGTATGGCT GCCGTGTTAT CCAGAAAGCT CTTGAGTTTA TTCCCTCAGA CCAGCAGGTA
    ATTAATGAAA TGGTTCGGGA ACTGGATGGC CACGTCCTCA AGTGTGTGAA AGACCAGAAT
    GGCAATCACG TGGTGCAGAA GTGCATCGAA TGTGTGCAGC CCCAGTCTCT GCAGTTCATC
    ATCGATGCGT TTAAGGGGCA GGTGTTTGCT TTGTCCACGC ATCCTTATGG CTGCCGAGTG
    ATTCAGAGGA TTCTGGAGCA CTGTCTCCCT GACCAGACAC TCCCTATTTT AGAGGAGCTT
    CACCAGCACA CAGAGCAACT TGTACAGGAT CAGTATGGAA ATTACGTAAT CCAGCATGTA
    TTGGAGCATG GTCGTCCTGA GGATAAAAGT AAAATTGTAG CAGAAATTCG AGGCAATGTA
    CTTGTGTTGA GTCAGCACAA ATTTGCAAGC AATGTTGTGG AGAAGTGTGT CACTCACGCC
    TCCCGTACAG AGCGCGCTGT GCTTATTGAC GAGGTGTGCA CCATGAACGA TGGTCCCCAC
    AGTGCCTTAT ACACCATGAT GAAGGACCAG TATGCCAACT ACGTGGTCCA GAAGATGATC
    GATGTGGCAG AGCCAGCCCA GAGGAAGATC GTCATGCACA AGATCCGGCC CCACATCGCG
    ACTCTTCGCA AGTATACTTA CGGCAAGCAC ATCCTGGCCA AGCTGGAGAA ATACTACATG
    AAGAATGGCG TTGACTTAGG GCCCATCTGC GGCCCCCCTA ATGGTATTAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16804
    Clone ID Related Accession (Same CDS sequence) XM_005202950.4 , XM_005202950.3
    Accession Version XM_005202950.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202950.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203007.1
    CDS134..3412
    Translation

    Target ORF information:

    RefSeq Version XM_005202950.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202950.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16804
    Clone ID Related Accession (Same CDS sequence) XM_005202950.4 , XM_005202950.3
    Accession Version XM_005202950.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203007.1
    CDS118..3396
    Translation

    Target ORF information:

    RefSeq Version XM_005202950.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202950.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGGGCCCAAG GGATGCAGAC AGTGATGAAA ACGACAAAGG TGAAAAGAAG
    AACAAGGGTA CGTTTGATGG AGATAAGCTA GGAGATTTGA AGGAGGAGGG TGATGTGATG
    GACAAGACCA ATGGTTTACC AGTGCAGAAT GGGATTGACG CAGACGTCAA AGATTTTAGC
    CGTACCCCTG GTAATTGCCA GAACTCTGCT AATGAAGTGG ATCTTCTGGG TCCAAACCAG
    AATGGTTCTG AGGGTTTAGC CCAGCTGACC AGCACCAATG GTGCCAAGCC TGTGGAGGAT
    TTCTCCAACA TGGAGTCCCA GAGTGTCCCC TTGGACCCCA TGGAACATGT GGGCATGGAG
    CCTCTTCAGT TTGATTATTC AGGCACGCAG GTACCTGTGG ACTCAGCAGC AGCAACTGTG
    GGACTTTTTG ACTACAATTC TCAACAACAG CTGTTCCAAA GACCCAATGC GCTTGCTGTC
    CAGCAGTTGA CAGCCGCTCA GCAGCAGCAG TATGCGTTAG CAGCCGCGCA TCAACCCCAC
    ATCGGTTTAG CTCCCGCTGC ATTCGTCCCC AACCCATATA TCATCAGTGC TGCGCCCCCA
    GGGACGGACC CCTACACAGC TGGATTGGCT GCAGCAGCGA CGCTAGGCCC GGCTGTGGTC
    CCTCCCCAGT ATTACGGAGT TACTCCCTGG GGAGTCTACC CTGCCAGTCT TTTCCAGCAG
    CAAGCCGCCG CCGCTGCTGC AGCAACAAAT TCTGCTAATC AGCAGACCAC CCCACAGGCT
    CAGCAAGGGC AGCAGCAGGT TCTCCGTGGA GGAGCCAGCC AGCGTCCTTT GACGCCGAAT
    CAGAACCAGC AGGGACAGCA AGCCGATCCA CTGGTGGCAG CTGCAGCGGT GAATTCTGCC
    CTTGCGTTTG GACAAGGACT GGCTGCGGGC ATGCCAGGTT ATCCAGTCTT GGCTCCTGCT
    GCTTACTATG ACCAAACCGG TGCCCTTGTG GTGAATGCAG GAGCGAGAAA TGGTCTTGGA
    GCTCCTGTTC GACTTGTAGC TCCCGCCCCA GTCATCATTA GTTCCTCAGC TGCACAAGCA
    GCTGTTGCAG CGGCCGCAGC TTCAGCAAAT GGAGCAGCCG GTGGTCTTGC TGGAACAACA
    AATGGACCAT TTCGCCCTTT AGGAACTCAG CAGCCTCAGC CCCAGCCCCA GCAGCAGCCC
    AGTAACAACC TGGCATCCAG CTCTTTCTAC GGCAACAACT CTCTGAGCAG CAATTCACAG
    AGCAGCTCCC TCTTCTCCCA GGGCTCCGCC CAGCCTGCCA ACACATCCTT GGGATTTGGA
    AGTAGCAGTT CTCTCGGCGC CACCCTGGGA TCAGCCCTTG GAGGGTTTGG AACAGCAGTT
    GCAAACTCCA ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT
    TATAAGAGGA CATCGAGCAG CTTGACCCCC ATTGGACACA GTTTTTATAA CGGCCTTAGC
    TTTTCCTCCT CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA
    CAGACACCAC CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA
    CTCACAAATG GCAGTGGAAG ATACATCTCT GCTGCTCCAG GCGCTGAAGC CAAGTACCGC
    AGCGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CCCTTTTTTC CTCCTCCCGT
    TTGCGATACG GAATGTCTGA TGTCATGCCC TCTGGCAGGA GCAGGCTTTT GGAAGATTTT
    CGAAATAACC GATATCCTAA TTTACAATTG CGGGAGATTG CTGGGCACAT AATGGAGTTT
    TCCCAAGACC AGCATGGTTC CAGATTCATT CAGCTGAAAC TTGAGCGTGC CACACCAGCC
    GAACGCCAAC TTGTCTTCAA TGAAATCCTT CAGGCCGCTT ACCAGCTGAT GGTGGATGTA
    TTTGGAAATT ACGTCATTCA GAAGTTCTTT GAATTTGGCA GCCTTGAACA GAAGCTGGCT
    TTGGCTGAGC GGATTCGAGG CCACGTCCTG TCATTGGCAC TGCAGATGTA TGGCTGCCGT
    GTTATCCAGA AAGCTCTTGA GTTTATTCCC TCAGACCAGC AGGTAATTAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53174
    Clone ID Related Accession (Same CDS sequence) XM_010802429.3 , XM_010802429.2
    Accession Version XM_010802429.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800731.1
    CDS134..3472
    Translation

    Target ORF information:

    RefSeq Version XM_010802429.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802429.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53174
    Clone ID Related Accession (Same CDS sequence) XM_010802429.3 , XM_010802429.2
    Accession Version XM_010802429.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800731.1
    CDS118..3456
    Translation

    Target ORF information:

    RefSeq Version XM_010802429.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGCGTTG CATGTGTCTT GAAGAGAAAA GCAGTGCTTT GGCAGGACTC TTTCAGCCCC 
    CACCTGAAAC ATCACCCTCA AGAACCAGCT AATCCCAACA TGCCTGTTGT TTTGACATCT
    GGAACAGGGT CGCAAGCGCA GCCACAACCA GCTGCAAATC AGGCTCTTGC AGCTGGGACA
    CACTCCAGCC CTGTCCCAGG ATCCATAGGA GTTGCAGGCC GTTCCCAGGA CGACGCTATG
    GTGGACTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAATAGCAA ACATCGATGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTACGTT CTATGGATGA ACTGAATCAT GATTTTCAAG CACTTGCTCT GGAGGGAAGA
    GCGATGGGAG AGCAGCTCTT GCCAGGTAAA AAGTTTTGGG AAACAGATGA ATCCAGCAAA
    GATGGACCAA AAGGAATATT CCTGGGTGAT CAATGGCGAG ACAGTGCCTG GGGAACATCA
    GATCATTCAG TTTCCCAGCC AATCATGGTG CAGAGAAGAC CTGGTCAGAG TTTCCATGTG
    AACAGTGAGG TCAATTCTGT ACTGTCCCCA CGGTCGGAGA GTGGGGGACT AGGCGTTAGC
    ATGGTGGAGT ATGTATTGAG CTCATCCCCG GGCGATTCCT GTCTAAGAAA AGGAGGATTT
    GGCCCAAGGG ATGCAGACAG TGATGAAAAC GACAAAGGTG AAAAGAAGAA CAAGGGTACG
    TTTGATGGAG ATAAGCTAGG AGATTTGAAG GAGGAGGGTG ATGTGATGGA CAAGACCAAT
    GGTTTACCAG TGCAGAATGG GATTGACGCA GACGTCAAAG ATTTTAGCCG TACCCCTGGT
    AATTGCCAGA ACTCTGCTAA TGAAGTGGAT CTTCTGGGTC CAAACCAGAA TGGTTCTGAG
    GGTTTAGCCC AGCTGACCAG CACCAATGGT GCCAAGCCTG TGGAGGATTT CTCCAACATG
    GAGTCCCAGA GTGTCCCCTT GGACCCCATG GAACATGTGG GCATGGAGCC TCTTCAGTTT
    GATTATTCAG GCACGCAGGT ACCTGTGGAC TCAGCAGCAG CAACTGTGGG ACTTTTTGAC
    TACAATTCTC AACAACAGCT GTTCCAAAGA CCCAATGCGC TTGCTGTCCA GCAGTTGACA
    GCCGCTCAGC AGCAGCAGTA TGCGTTAGCA GCCGCGCATC AACCCCACAT CGGTATGTTT
    TCAGCAGGTT TAGCTCCCGC TGCATTCGTC CCCAACCCAT ATATCATCAG TGCTGCGCCC
    CCAGGGACGG ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACGCTAGG CCCGGCTGTG
    GTCCCTCCCC AGTATTACGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG
    CAGCAAGCCG CCGCCGCTGC TGCAGCAACA AATTCTGCTA ATCAGCAGAC CACCCCACAG
    GCTCAGCAAG GGCAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAGCGTCC TTTGACGCCG
    AATCAGAACC AGCAGGGACA GCAAGCCGAT CCACTGGTGG CAGCTGCAGC GGTGAATTCT
    GCCCTTGCGT TTGGACAAGG ACTGGCTGCG GGCATGCCAG GTTATCCAGT CTTGGCTCCT
    GCTGCTTACT ATGACCAAAC CGGTGCCCTT GTGGTGAATG CAGGAGCGAG AAATGGTCTT
    GGAGCTCCTG TTCGACTTGT AGCTCCCGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA
    GCAGCTGTTG CAGCGGCCGC AGCTTCAGCA AATGGAGCAG CCGGTGGTCT TGCTGGAACA
    ACAAATGGAC CATTTCGCCC TTTAGGAACT CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG
    CCCAGTAACA ACCTGGCATC CAGCTCTTTC TACGGCAACA ACTCTCTGAG CAGCAATTCA
    CAGAGCAGCT CCCTCTTCTC CCAGGGCTCC GCCCAGCCTG CCAACACATC CTTGGGATTT
    GGAAGTAGCA GTTCTCTCGG CGCCACCCTG GGATCAGCCC TTGGAGGGTT TGGAACAGCA
    GGAGGACTCA CAAATGGCAG TGGAAGATAC ATCTCTGCTG CTCCAGGCGC TGAAGCCAAG
    TACCGCAGCG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACCCT TTTTTCCTCC
    TCCCGTTTGC GATACGGAAT GTCTGATGTC ATGCCCTCTG GCAGGAGCAG GCTTTTGGAA
    GATTTTCGAA ATAACCGATA TCCTAATTTA CAATTGCGGG AGATTGCTGG GCACATAATG
    GAGTTTTCCC AAGACCAGCA TGGTTCCAGA TTCATTCAGC TGAAACTTGA GCGTGCCACA
    CCAGCCGAAC GCCAACTTGT CTTCAATGAA ATCCTTCAGG CCGCTTACCA GCTGATGGTG
    GATGTATTTG GAAATTACGT CATTCAGAAG TTCTTTGAAT TTGGCAGCCT TGAACAGAAG
    CTGGCTTTGG CTGAGCGGAT TCGAGGCCAC GTCCTGTCAT TGGCACTGCA GATGTATGGC
    TGCCGTGTTA TCCAGAAAGC TCTTGAGTTT ATTCCCTCAG ACCAGCAGAA TGAAATGGTT
    CGGGAACTGG ATGGCCACGT CCTCAAGTGT GTGAAAGACC AGAATGGCAA TCACGTGGTG
    CAGAAGTGCA TCGAATGTGT GCAGCCCCAG TCTCTGCAGT TCATCATCGA TGCGTTTAAG
    GGGCAGGTGT TTGCTTTGTC CACGCATCCT TATGGCTGCC GAGTGATTCA GAGGATTCTG
    GAGCACTGTC TCCCTGACCA GACACTCCCT ATTTTAGAGG AGCTTCACCA GCACACAGAG
    CAACTTGTAC AGGATCAGTA TGGAAATTAC GTAATCCAGC ATGTATTGGA GCATGGTCGT
    CCTGAGGATA AAAGTAAAAT TGTAGCAGAA ATTCGAGGCA ATGTACTTGT GTTGAGTCAG
    CACAAATTTG CAAGCAATGT TGTGGAGAAG TGTGTCACTC ACGCCTCCCG TACAGAGCGC
    GCTGTGCTTA TTGACGAGGT GTGCACCATG AACGATGGTC CCCACAGTGC CTTATACACC
    ATGATGAAGG ACCAGTATGC CAACTACGTG GTCCAGAAGA TGATCGATGT GGCAGAGCCA
    GCCCAGAGGA AGATCGTCAT GCACAAGATC CGGCCCCACA TCGCGACTCT TCGCAAGTAT
    ACTTACGGCA AGCACATCCT GGCCAAGCTG GAGAAATACT ACATGAAGAA TGGCGTTGAC
    TTAGGGCCCA TCTGCGGCCC CCCTAATGGT ATTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.