POLQ cDNA ORF clone, Bos taurus(cattle)

The following POLQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219127 XM_024994620.1
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa87725 XM_015462017.1
Latest version!
Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa87725 XM_015471622.2
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa02530 XM_615375.6
Latest version!
Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa02530 XM_002684789.5
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53126 XM_010824625.2
Latest version!
Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53126 XM_010801194.3
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53127 XM_010824626.2
Latest version!
Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53127 XM_010801195.3
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53128 XM_010801196.3
Latest version!
Bos taurus DNA polymerase theta (POLQ), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53128 XM_010824627.2
Latest version!
Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OBa53126 XM_010801194.2 Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa87725 XM_015471622.1 Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa53127 XM_010801195.2 Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa02530 XM_002684789.4 Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa53128 XM_010801196.2 Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219127
    Clone ID Related Accession (Same CDS sequence) XM_024994620.1
    Accession Version XM_024994620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTGAGAGTA TCTTAGTTTG TAAAAACTCT GAGAAATCAA AAGGCATAAC TCTACTTCAG
    GGATCTCTGA AACCTGTTCA TAGCTGTTTG CAAAGACACG AAGGAGAAGA AGTAACCTCA
    AGCATGATAA GAGCTATTTT GGAGATAATA GTTGGTGGAG TGGCAAGTAC GTCACAAGAT
    ATACAGACTT ATGCTTCTTG CACATTTTTG GCTGCAAGTA TGAAAGAAGG GAAGCAGGGG
    ATTCAGAAAA ATAAAGATTC TGTTCAACTT GGAGCAATTG AGGCCTGTGT AATGTGGCTA
    CTAGAAAATG AATTCATCCA GATTACTGAA GCTAGTGATG GAACAGAAGG AAATGTGTAT
    CATCCAACAC ACCTTGGTTC CGCAACTCTT TCTTCTTCAC TGTCTCCAAT TGATACTTTA
    GATATTTTTG CTGACCTACA AAGAGCAATG AAGGGCTTTG TTTTAGAGAA TGATCTGCAT
    ATTGTTTATT TGGTTACACC TGTGTTTGAG GATTGGACTA CTATTGATTG GTATCGATTT
    TTCTGTTTAT GGGAGAAGTT GCCAGCTTCT ATGAAGCGGG TGGCAGAGCT AGTAGGAGTT
    GAAGAAGGGT TCTTGGCTCG CTGTGTGAAA GGAAATTTAG TAGCCAGAAC TGAGAGACAG
    CACCGACAAA TGGCCATCCA TAAAAGGTTT TTTACCAGTC TTGTGCTATT AGATTTAATC
    AGTGAAGTTC CCTTAAAGGA AATTAATCAG AAATATGGAT GCAATCGTGG ACAGATTCAG
    TCATTACAAC AGTCAGCTGC TGTTTATGCA GGGATGATTA CAGTGTTTTC CAACCGCCTG
    GGCTGGCACA ACATGGAACT ACTGCTTTCC CAATTCCAGA AGCGTCTTAC ATTTGGCATC
    CAAAGGGAGC TGTGTGACCT GGTCCGGGTG TCATTACTTA ATGCACAGCG GGCCAGATTC
    CTCTATGCAT CTGGTTTTCT TACTGTGTCT GATCTTGCTA GAGCAGATGT TGCTGAGGTG
    GAAAGTGTTC TGAAAAATGC TGCACCTTTT AAAAGTACTC GAAAGGCAGT GGATGAGGAA
    GAGGAAGCAG TTGAAGAGCG TCGAAATATG CGAACTATTT GGGTGACAGG CAGAAAAGGT
    TTAAGTGAAA GGGAAGCAGC AACCCTTATA GTAGAAGAAG CCAGAATGAT TCTGCAGCAG
    GACTTAGTTG AAATGGGAGT GCAATGGAAT CTACATTCCT TTCTAAATTC TGATACACAC
    TTACTGACCA GCAGTGAGTC AGAAGTAAAA GAACACAAAT TTATACCTCA AACTAAGAGT
    TCTTATAAAA GATTAACATC AAAGAAAAAA AGTGACACAA TATTTAGTGA TTCTGTTAAT
    CACTTACCAG ATACCATGCA AGATTTAGAT AAAAGTAGAG AACATACAGA TTCCTTTTAT
    TATAAATTTC AGGATGGGAA TCAAGAGTGT CATCATTCCA TCTCCAGAGG GAAAAAACAG
    GCTTCTTTGA GTATTAAAAA AGGAAAGCTA GGAACTTCTC TGAATGAGGG GAAAGCAAGC
    ACTTCGAAAG TTGTTCGGAC TCTCTCATCA CGGAAAACAA AAAGAGAAGC TTTAAATTTT
    AGTTCAGAAA AAGTGAGTAC AATCTCTCAA TCTTGGAAAC ATGGTAAGCA TCGAAAACGA
    TCCAGCGACA GTAGCCCTGT GAAAGACACA AAGATGCGTA GAATCAATTT ACAAGAACGG
    ACTATGTCTA ATTCTATTCA TTGTGAAGAC TCTTTTACTT TAGATAAGAT GAGTATGGAA
    TTTAGAAGTC CAGAGCCATT TGCTAAAAAT GTATCTTTCC GTGCTGAGAG AAGATGTAAC
    ATAATACCAT TTGCATCACA AACAGAACAA AGTTGTTCTA GGAACAATAA AATACCTAAT
    GATATTCTTG GGGAACACTT GATCACAGGA TCTCAGAGTA AACATGTGAT TCAAGCCACT
    AGTGTGGTCA GTGAAAATGG AAGAGAATTA GCTATTGAAT CAGAAAAAAA AAATAAAGTG
    GTGATACAAA ATGATTCAAA AATCCAGAAT GTTTATGTGA AACACCATGA CACCCATCCA
    ATTAACCAGT GCCTGGAAAA GCAGTTTAAT AAAGAGACAA ATACTCACAT CAAACTGAAT
    ACAACAGTAG CAAAACAAAT ACCCTATGAG ACAGACAGTA GTTATTTGAA TAGAGACTCA
    AATGTTATTA TGAAATGTGA AAGTACAAAG TTTAATGTTG AGGAAAATAA ATCAAGTAAT
    CTTAAGGCAT CAGGAGATAT CATAAGCAGC ATTGAGATAC CCAGTGAAGT ACATCTACCA
    AGTGGAGCAT TTGGTAAATT AAGAGGCCAG CCTGAGAATA TTCTAAATAC TCCTAGAGTA
    CAAGGAAGAA CAGATGCTTA TGCAGCAGAC AGAACTAAAA ATAATTGTGT TTCTGACTTA
    GGTTTAGATC TCTGTGATCT TGAAGATAGT TTCTACCTGG ATACTCAGTC AGAGAAAATA
    ATTCAGCAGA TAGCAACTGC AAATGCCAAC CAAGAAGGAC CCAAGGATAC CAGCCTGGCA
    ACAGGGATGA TTCAGAAGAA CCTAGACAAA CAGAGCTCAA TGAGCTCCTT TCAGAAAGAG
    CCTCATGTTG CTTTTCCAGG CGAACAGCAT TCACTAGAAA TGACAAATCA TTTGAAACAT
    AAGACTGTAG ATGTTGTGAA GCAAAGCACT GATTCACATG AGGTTGATAC CCTGACTCCA
    GAAAGGCCAA TTTTTTATTC TCCAGTACTA GTAGGGGAAA ATGGCCCATG TTTTAAAGGC
    AGTGAACTTT CTGTTACTGA CTCCCAATTA AATAGTTTTC TTCAAGGTTA TCAAACACAA
    GAAACTATGA AGCCAGTTAT ACCTGTGATT CCTCAAAAGA GAATTCCTAC TGATACAGAA
    GTAGAGTGTC TGCCAGTTCC TGAAACAAGT TTGAATTTGA GTGGTAGTTT ACTGTTTGAC
    AGTTTCAGTG AAGACTACCT TGTAGAAGAA CAACGACCTG ATGGACAAGA AAAAGAACAC
    CTTCCTTCTG AAATAACATC AGTCCATTTC GATGATTCTC TGTGTCTACA ACCAGAGGGC
    ATAATTAAAA AACCAGATAT GAATGAGAAT CAAGATAATC AGCAGCCATT GTCTTGTTTC
    AGTGATGAGT CTATTATATT TTCAGAAATC GATTCTGTCC AGATGGTTGC AGCTTTGGAC
    AATGTAGACA TAGTTCCTGT GCAGGAGAAA CATAATACTG CAGAATCTCT AAAAATACTG
    AAACGGAGTG ATCAAGTGAC TGTAGGTAAT GATGATTCCC AAAGAGAAGT AATTGGAGAA
    GAAAAAGATA AAAGGTCTCA AAAATCCAAG TTAACTGGCA CAAGGCAAGA TAATTCATTT
    CTATGGTCAG GAGCATCATT TGATTTAAGT CCAGGACTGC AAAGGATTTT AGACCAAGTG
    TCCAGTCCTC TAGAAAATGA AAAGCCAAAA TTAACTGCTA CAGACTTGCC TGGTTTGAAT
    GGAGAAAATG CAGAGGTAAA TGACAGACAA GTGATTTCAA ATACGGAGCC AAATCAAGTA
    CAAGGAATTC CATTTTCACC TAAAATTGTA GTAAGAGGCA ATCTTGAGGC ACTACAGAAC
    GATGTCATTC ATGGTGAAAC CTCCCTCTTG CCTTGTAAAG AAAACTGTAT AGTTCGTGAT
    AATGGACTCA TTCCTCCTAC ACCTATTCCT TCTGATTCTA AGTTGGCATT TCCAGATATT
    CTTGGAACAT CTGTGAAACT TAAGAAAATT AGTCATGGTT TTAAACTTGG TGAAAGTTCT
    TCATTTTGCT CACCTTCCAA TAATAAAAAC CATGATTTAC ATCCAGAGAG TAGAAATGGT
    TTTAAAGATA ACAGTTCTAT TAGAGACACA AATTTTTCAC CGCAGTTACT GCAGAATGGA
    TCACAGTTAA CTCTAGCTTC ATGCAGTCCT GAAAGTTTGG CCATAATTGA TGTAGCAAGT
    AACCAAACTC TCTTTCAAAC ATTCATGAAG GAATGGCAAA GCAAAACTCG ATTCTCCATC
    TCACTGGCTT GTGAAAAGAT CAGTAATTTG ACATCTTCTA AAAGTGCTAC TATTGGTGGT
    ATGTTTAAGC AAGTTAGTTT CCCTCAAGAA GCCTCTATTA GAGATGATGG ATTTCCTGTT
    AAAGACTGTG ATGACATCTT ACTGGTTGGG CTTGCAGTAT GTTGGGGTGG AAAAGATGCT
    TACTATTTTT CACTGCAGAA GGAACAAAAG CATTCTGAAA TTAGTGCCAG TTTGATCCCA
    CCTTCTCTGG ATCCAAGCCT GACTGTGAAG GATAGATTAC GGCACCTTCA ATCTTGCTTT
    CACAAAGAAT CTGATAAAGA ACGTTCTGTT ATCATCTATG ACTTCATCCA GAGTTATAAA
    ATTCTTCTTC TGTCTTGTGG CATCTCCCTG GAACAAAGTT ACGAAGATCC TAAGGTGGCA
    TGCTGGTTAC TAGATCCAGA TTCCAAGGAG CCGACTCTTC ACAGCATAGT TTCCCATTTT
    CTTCCTCATG AGCTTCCACT CCTGGAAGAA ATAGAGACTG GCCAAGGAAT TCAAAGTCTA
    GGGCTGAATG TCAGCACTAC GCATTCTGGA CGATACAGAG CATCTGTAGA GTCCATTCTC
    ATCTTCAACT CTATGAATCA ACTTAATTCT TTGTTGAAGA AGGAAAACCT TCAAGATGTT
    TTCTATAAAG TGGAAATGCC CTCCCAGTAC TGCCTAGCCT TGCTGGAACT AAATGGAATC
    GGCTTTAGTA CTGCAGAATG TGAAAGTCAG AAACACATAA TGCAAGCCAA ACTGGATGCA
    ATTGAGATGC AGGCCTATCA ACTAGCTGGC CATAGTTTTT CCTTCACCAG TTCAGATGAC
    ATTGCTGAGG TTTTATTTTT GGAACTAAAG TTGCCACCAA ATGGAGAGGG AAAAAACCAA
    GGCATCAAGA AAACTTTGGG TTCTACCAGA AGAGGAAATG ACAATGGACG GAAGCTAAGG
    CTGGGAAAAC AGTTCAGCAC TAGTAAGGGT GTTTTAAATA AATTAAGGGC ATTACATCCT
    TTACCAGGCT TGATCTTAGA GTGGAGAAGA ATCACTAATG CTATTACCAA AGTGGTCTTT
    CCCCTGCAGC GGGAAAAACG TCTGAATCCT TTTCTTGGAA TGGAAAGAAT CTATCCTGTA
    TCACAATCAC ACACTGCTAC AGGACGAATA ACCTTTATAG AGCCAAATAT TCAGAATGTA
    CCAAGAGATT TTGAGATAAA AATGCCAGCA GTAGTAGAGG AAAGCCCACC TTCCCAAGCT
    CTAGGAAAAG GCCTACTTCC TATGGGCAGA GGAGGAAGAA AGAAGGACTG TGCCATGAAC
    GCTGGGCGCC AGACAAAGGT GGAAGAGAGA GCCTCAGACA GAGGAATGCC CTTCTCGGTC
    AGCATGCGAC ATGCCTTTGT GCCTTTCACA GGTGGTTTAA TATTAGCGGC TGATTATTCT
    CAACTTGAGC TGAGGATCTT GGCTCATTTA TCCCACGATC GTCGTCTCAT TCAAGTGCTA
    AACACTGGAG CTGATGTTTT CAGGAGTATT GCAGCTGAAT GGAAGATGAT TGAGCCAGAG
    TCTGTTGGGG ATAATCTGAG GCAACAAGCA AAGCAGATTT GCTATGGAAT CATTTATGGA
    ATGGGAGCTA AGTCTTTGGG AGAGCAGATG GGTATTAAAG AAAACGATGC TGCTTGCTAC
    ATTGACTCCT TCAAATCCAG ATACACGGGG ATTAGTCATT TCATGAGAGA GACCGTGAAG
    AACTGTAAAA GAGATGGATT TGTTCAGACC ATTTTGGGAA GGCGTAGATA TTTACCAGGA
    ATCAAAGACA ACAACCCTTA TCATAAAGCT CATGCTGAGC GTCAGGCTAT CAACACAAGA
    GTCCAAGGTT CGGCGGCTGA TATTGTCAAA ATAGCCACAG TTAATATTCA GAAGCAGTTA
    GAGACCTTCC ACTCAACTTT CAAATCCCAT GGTCATCGAG AGGGTTTGCT CCAAAGTGAA
    AGAACAGGAT TGTTGCCAAA GAAAAAACTG CAAGGGATGT TCTGCCCCAT CAGAGGAGGC
    TTCTTTATCC TTCAGCTTCA TGATGAACTC TTATATGAAG TGGCAGAAGA AGACGTTGTT
    CAGGTAGCTC AGATTGTCAA GAATGAAATG GAAAGTGCCA TAAAACTGTC TGTGAAACTG
    AGAGTGAAAG TGAGGATAGG TGCCAGCTGG GGGGAGCTGA AGGACTTTGA CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850388.1
    CDS425..8041
    Translation

    Target ORF information:

    RefSeq Version XM_024994620.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTGAGAGTA TCTTAGTTTG TAAAAACTCT GAGAAATCAA AAGGCATAAC TCTACTTCAG
    GGATCTCTGA AACCTGTTCA TAGCTGTTTG CAAAGACACG AAGGAGAAGA AGTAACCTCA
    AGCATGATAA GAGCTATTTT GGAGATAATA GTTGGTGGAG TGGCAAGTAC GTCACAAGAT
    ATACAGACTT ATGCTTCTTG CACATTTTTG GCTGCAAGTA TGAAAGAAGG GAAGCAGGGG
    ATTCAGAAAA ATAAAGATTC TGTTCAACTT GGAGCAATTG AGGCCTGTGT AATGTGGCTA
    CTAGAAAATG AATTCATCCA GATTACTGAA GCTAGTGATG GAACAGAAGG AAATGTGTAT
    CATCCAACAC ACCTTGGTTC CGCAACTCTT TCTTCTTCAC TGTCTCCAAT TGATACTTTA
    GATATTTTTG CTGACCTACA AAGAGCAATG AAGGGCTTTG TTTTAGAGAA TGATCTGCAT
    ATTGTTTATT TGGTTACACC TGTGTTTGAG GATTGGACTA CTATTGATTG GTATCGATTT
    TTCTGTTTAT GGGAGAAGTT GCCAGCTTCT ATGAAGCGGG TGGCAGAGCT AGTAGGAGTT
    GAAGAAGGGT TCTTGGCTCG CTGTGTGAAA GGAAATTTAG TAGCCAGAAC TGAGAGACAG
    CACCGACAAA TGGCCATCCA TAAAAGGTTT TTTACCAGTC TTGTGCTATT AGATTTAATC
    AGTGAAGTTC CCTTAAAGGA AATTAATCAG AAATATGGAT GCAATCGTGG ACAGATTCAG
    TCATTACAAC AGTCAGCTGC TGTTTATGCA GGGATGATTA CAGTGTTTTC CAACCGCCTG
    GGCTGGCACA ACATGGAACT ACTGCTTTCC CAATTCCAGA AGCGTCTTAC ATTTGGCATC
    CAAAGGGAGC TGTGTGACCT GGTCCGGGTG TCATTACTTA ATGCACAGCG GGCCAGATTC
    CTCTATGCAT CTGGTTTTCT TACTGTGTCT GATCTTGCTA GAGCAGATGT TGCTGAGGTG
    GAAAGTGTTC TGAAAAATGC TGCACCTTTT AAAAGTACTC GAAAGGCAGT GGATGAGGAA
    GAGGAAGCAG TTGAAGAGCG TCGAAATATG CGAACTATTT GGGTGACAGG CAGAAAAGGT
    TTAAGTGAAA GGGAAGCAGC AACCCTTATA GTAGAAGAAG CCAGAATGAT TCTGCAGCAG
    GACTTAGTTG AAATGGGAGT GCAATGGAAT CTACATTCCT TTCTAAATTC TGATACACAC
    TTACTGACCA GCAGTGAGTC AGAAGTAAAA GAACACAAAT TTATACCTCA AACTAAGAGT
    TCTTATAAAA GATTAACATC AAAGAAAAAA AGTGACACAA TATTTAGTGA TTCTGTTAAT
    CACTTACCAG ATACCATGCA AGATTTAGAT AAAAGTAGAG AACATACAGA TTCCTTTTAT
    TATAAATTTC AGGATGGGAA TCAAGAGTGT CATCATTCCA TCTCCAGAGG GAAAAAACAG
    GCTTCTTTGA GTATTAAAAA AGGAAAGCTA GGAACTTCTC TGAATGAGGG GAAAGCAAGC
    ACTTCGAAAG TTGTTCGGAC TCTCTCATCA CGGAAAACAA AAAGAGAAGC TTTAAATTTT
    AGTTCAGAAA AAGTGAGTAC AATCTCTCAA TCTTGGAAAC ATGGTAAGCA TCGAAAACGA
    TCCAGCGACA GTAGCCCTGT GAAAGACACA AAGATGCGTA GAATCAATTT ACAAGAACGG
    ACTATGTCTA ATTCTATTCA TTGTGAAGAC TCTTTTACTT TAGATAAGAT GAGTATGGAA
    TTTAGAAGTC CAGAGCCATT TGCTAAAAAT GTATCTTTCC GTGCTGAGAG AAGATGTAAC
    ATAATACCAT TTGCATCACA AACAGAACAA AGTTGTTCTA GGAACAATAA AATACCTAAT
    GATATTCTTG GGGAACACTT GATCACAGGA TCTCAGAGTA AACATGTGAT TCAAGCCACT
    AGTGTGGTCA GTGAAAATGG AAGAGAATTA GCTATTGAAT CAGAAAAAAA AAATAAAGTG
    GTGATACAAA ATGATTCAAA AATCCAGAAT GTTTATGTGA AACACCATGA CACCCATCCA
    ATTAACCAGT GCCTGGAAAA GCAGTTTAAT AAAGAGACAA ATACTCACAT CAAACTGAAT
    ACAACAGTAG CAAAACAAAT ACCCTATGAG ACAGACAGTA GTTATTTGAA TAGAGACTCA
    AATGTTATTA TGAAATGTGA AAGTACAAAG TTTAATGTTG AGGAAAATAA ATCAAGTAAT
    CTTAAGGCAT CAGGAGATAT CATAAGCAGC ATTGAGATAC CCAGTGAAGT ACATCTACCA
    AGTGGAGCAT TTGGTAAATT AAGAGGCCAG CCTGAGAATA TTCTAAATAC TCCTAGAGTA
    CAAGGAAGAA CAGATGCTTA TGCAGCAGAC AGAACTAAAA ATAATTGTGT TTCTGACTTA
    GGTTTAGATC TCTGTGATCT TGAAGATAGT TTCTACCTGG ATACTCAGTC AGAGAAAATA
    ATTCAGCAGA TAGCAACTGC AAATGCCAAC CAAGAAGGAC CCAAGGATAC CAGCCTGGCA
    ACAGGGATGA TTCAGAAGAA CCTAGACAAA CAGAGCTCAA TGAGCTCCTT TCAGAAAGAG
    CCTCATGTTG CTTTTCCAGG CGAACAGCAT TCACTAGAAA TGACAAATCA TTTGAAACAT
    AAGACTGTAG ATGTTGTGAA GCAAAGCACT GATTCACATG AGGTTGATAC CCTGACTCCA
    GAAAGGCCAA TTTTTTATTC TCCAGTACTA GTAGGGGAAA ATGGCCCATG TTTTAAAGGC
    AGTGAACTTT CTGTTACTGA CTCCCAATTA AATAGTTTTC TTCAAGGTTA TCAAACACAA
    GAAACTATGA AGCCAGTTAT ACCTGTGATT CCTCAAAAGA GAATTCCTAC TGATACAGAA
    GTAGAGTGTC TGCCAGTTCC TGAAACAAGT TTGAATTTGA GTGGTAGTTT ACTGTTTGAC
    AGTTTCAGTG AAGACTACCT TGTAGAAGAA CAACGACCTG ATGGACAAGA AAAAGAACAC
    CTTCCTTCTG AAATAACATC AGTCCATTTC GATGATTCTC TGTGTCTACA ACCAGAGGGC
    ATAATTAAAA AACCAGATAT GAATGAGAAT CAAGATAATC AGCAGCCATT GTCTTGTTTC
    AGTGATGAGT CTATTATATT TTCAGAAATC GATTCTGTCC AGATGGTTGC AGCTTTGGAC
    AATGTAGACA TAGTTCCTGT GCAGGAGAAA CATAATACTG CAGAATCTCT AAAAATACTG
    AAACGGAGTG ATCAAGTGAC TGTAGGTAAT GATGATTCCC AAAGAGAAGT AATTGGAGAA
    GAAAAAGATA AAAGGTCTCA AAAATCCAAG TTAACTGGCA CAAGGCAAGA TAATTCATTT
    CTATGGTCAG GAGCATCATT TGATTTAAGT CCAGGACTGC AAAGGATTTT AGACCAAGTG
    TCCAGTCCTC TAGAAAATGA AAAGCCAAAA TTAACTGCTA CAGACTTGCC TGGTTTGAAT
    GGAGAAAATG CAGAGGTAAA TGACAGACAA GTGATTTCAA ATACGGAGCC AAATCAAGTA
    CAAGGAATTC CATTTTCACC TAAAATTGTA GTAAGAGGCA ATCTTGAGGC ACTACAGAAC
    GATGTCATTC ATGGTGAAAC CTCCCTCTTG CCTTGTAAAG AAAACTGTAT AGTTCGTGAT
    AATGGACTCA TTCCTCCTAC ACCTATTCCT TCTGATTCTA AGTTGGCATT TCCAGATATT
    CTTGGAACAT CTGTGAAACT TAAGAAAATT AGTCATGGTT TTAAACTTGG TGAAAGTTCT
    TCATTTTGCT CACCTTCCAA TAATAAAAAC CATGATTTAC ATCCAGAGAG TAGAAATGGT
    TTTAAAGATA ACAGTTCTAT TAGAGACACA AATTTTTCAC CGCAGTTACT GCAGAATGGA
    TCACAGTTAA CTCTAGCTTC ATGCAGTCCT GAAAGTTTGG CCATAATTGA TGTAGCAAGT
    AACCAAACTC TCTTTCAAAC ATTCATGAAG GAATGGCAAA GCAAAACTCG ATTCTCCATC
    TCACTGGCTT GTGAAAAGAT CAGTAATTTG ACATCTTCTA AAAGTGCTAC TATTGGTGGT
    ATGTTTAAGC AAGTTAGTTT CCCTCAAGAA GCCTCTATTA GAGATGATGG ATTTCCTGTT
    AAAGACTGTG ATGACATCTT ACTGGTTGGG CTTGCAGTAT GTTGGGGTGG AAAAGATGCT
    TACTATTTTT CACTGCAGAA GGAACAAAAG CATTCTGAAA TTAGTGCCAG TTTGATCCCA
    CCTTCTCTGG ATCCAAGCCT GACTGTGAAG GATAGATTAC GGCACCTTCA ATCTTGCTTT
    CACAAAGAAT CTGATAAAGA ACGTTCTGTT ATCATCTATG ACTTCATCCA GAGTTATAAA
    ATTCTTCTTC TGTCTTGTGG CATCTCCCTG GAACAAAGTT ACGAAGATCC TAAGGTGGCA
    TGCTGGTTAC TAGATCCAGA TTCCAAGGAG CCGACTCTTC ACAGCATAGT TTCCCATTTT
    CTTCCTCATG AGCTTCCACT CCTGGAAGAA ATAGAGACTG GCCAAGGAAT TCAAAGTCTA
    GGGCTGAATG TCAGCACTAC GCATTCTGGA CGATACAGAG CATCTGTAGA GTCCATTCTC
    ATCTTCAACT CTATGAATCA ACTTAATTCT TTGTTGAAGA AGGAAAACCT TCAAGATGTT
    TTCTATAAAG TGGAAATGCC CTCCCAGTAC TGCCTAGCCT TGCTGGAACT AAATGGAATC
    GGCTTTAGTA CTGCAGAATG TGAAAGTCAG AAACACATAA TGCAAGCCAA ACTGGATGCA
    ATTGAGATGC AGGCCTATCA ACTAGCTGGC CATAGTTTTT CCTTCACCAG TTCAGATGAC
    ATTGCTGAGG TTTTATTTTT GGAACTAAAG TTGCCACCAA ATGGAGAGGG AAAAAACCAA
    GGCATCAAGA AAACTTTGGG TTCTACCAGA AGAGGAAATG ACAATGGACG GAAGCTAAGG
    CTGGGAAAAC AGTTCAGCAC TAGTAAGGGT GTTTTAAATA AATTAAGGGC ATTACATCCT
    TTACCAGGCT TGATCTTAGA GTGGAGAAGA ATCACTAATG CTATTACCAA AGTGGTCTTT
    CCCCTGCAGC GGGAAAAACG TCTGAATCCT TTTCTTGGAA TGGAAAGAAT CTATCCTGTA
    TCACAATCAC ACACTGCTAC AGGACGAATA ACCTTTATAG AGCCAAATAT TCAGAATGTA
    CCAAGAGATT TTGAGATAAA AATGCCAGCA GTAGTAGAGG AAAGCCCACC TTCCCAAGCT
    CTAGGAAAAG GCCTACTTCC TATGGGCAGA GGAGGAAGAA AGAAGGACTG TGCCATGAAC
    GCTGGGCGCC AGACAAAGGT GGAAGAGAGA GCCTCAGACA GAGGAATGCC CTTCTCGGTC
    AGCATGCGAC ATGCCTTTGT GCCTTTCACA GGTGGTTTAA TATTAGCGGC TGATTATTCT
    CAACTTGAGC TGAGGATCTT GGCTCATTTA TCCCACGATC GTCGTCTCAT TCAAGTGCTA
    AACACTGGAG CTGATGTTTT CAGGAGTATT GCAGCTGAAT GGAAGATGAT TGAGCCAGAG
    TCTGTTGGGG ATAATCTGAG GCAACAAGCA AAGCAGATTT GCTATGGAAT CATTTATGGA
    ATGGGAGCTA AGTCTTTGGG AGAGCAGATG GGTATTAAAG AAAACGATGC TGCTTGCTAC
    ATTGACTCCT TCAAATCCAG ATACACGGGG ATTAGTCATT TCATGAGAGA GACCGTGAAG
    AACTGTAAAA GAGATGGATT TGTTCAGACC ATTTTGGGAA GGCGTAGATA TTTACCAGGA
    ATCAAAGACA ACAACCCTTA TCATAAAGCT CATGCTGAGC GTCAGGCTAT CAACACAAGA
    GTCCAAGGTT CGGCGGCTGA TATTGTCAAA ATAGCCACAG TTAATATTCA GAAGCAGTTA
    GAGACCTTCC ACTCAACTTT CAAATCCCAT GGTCATCGAG AGGGTTTGCT CCAAAGTGAA
    AGAACAGGAT TGTTGCCAAA GAAAAAACTG CAAGGGATGT TCTGCCCCAT CAGAGGAGGC
    TTCTTTATCC TTCAGCTTCA TGATGAACTC TTATATGAAG TGGCAGAAGA AGACGTTGTT
    CAGGTAGCTC AGATTGTCAA GAATGAAATG GAAAGTGCCA TAAAACTGTC TGTGAAACTG
    AGAGTGAAAG TGAGGATAGG TGCCAGCTGG GGGGAGCTGA AGGACTTTGA CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87725
    Clone ID Related Accession (Same CDS sequence) XM_015471622.1 , XM_015471622.2 , XM_015462017.1
    Accession Version XM_015471622.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327108.1
    CDS366..6926
    Translation

    Target ORF information:

    RefSeq Version XM_015471622.1
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87725
    Clone ID Related Accession (Same CDS sequence) XM_015471622.1 , XM_015471622.2 , XM_015462017.1
    Accession Version XM_015471622.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327108.1
    CDS425..6985
    Translation

    Target ORF information:

    RefSeq Version XM_015471622.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471622.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87725
    Clone ID Related Accession (Same CDS sequence) XM_015471622.1 , XM_015471622.2 , XM_015462017.1
    Accession Version XM_015462017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317503.1
    CDS366..6926
    Translation

    Target ORF information:

    RefSeq Version XM_015462017.1
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGGT GTTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02530
    Clone ID Related Accession (Same CDS sequence) XM_615375.6 , XM_002684789.4 , XM_002684789.5
    Accession Version XM_615375.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_615375.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_615375.3
    CDS362..8143
    Translation

    Target ORF information:

    RefSeq Version XM_615375.6
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_615375.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02530
    Clone ID Related Accession (Same CDS sequence) XM_615375.6 , XM_002684789.4 , XM_002684789.5
    Accession Version XM_002684789.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002684835.1
    CDS362..8143
    Translation

    Target ORF information:

    RefSeq Version XM_002684789.4
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002684789.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02530
    Clone ID Related Accession (Same CDS sequence) XM_615375.6 , XM_002684789.4 , XM_002684789.5
    Accession Version XM_002684789.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002684789.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002684835.1
    CDS425..8206
    Translation

    Target ORF information:

    RefSeq Version XM_002684789.5
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002684789.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGGT AGCTCAGATT
    GTCAAGAATG AAATGGAAAG TGCCATAAAA CTGTCTGTGA AACTGAGAGT GAAAGTGAGG
    ATAGGTGCCA GCTGGGGGGA GCTGAAGGAC TTTGACGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53126
    Clone ID Related Accession (Same CDS sequence) XM_010824625.2 , XM_010801194.2 , XM_010801194.3
    Accession Version XM_010824625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822927.1
    CDS363..8048
    Translation

    Target ORF information:

    RefSeq Version XM_010824625.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53126
    Clone ID Related Accession (Same CDS sequence) XM_010824625.2 , XM_010801194.2 , XM_010801194.3
    Accession Version XM_010801194.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799496.1
    CDS363..8048
    Translation

    Target ORF information:

    RefSeq Version XM_010801194.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801194.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53126
    Clone ID Related Accession (Same CDS sequence) XM_010824625.2 , XM_010801194.2 , XM_010801194.3
    Accession Version XM_010801194.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799496.1
    CDS425..8110
    Translation

    Target ORF information:

    RefSeq Version XM_010801194.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801194.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGATT GGTAAGATCA
    TCTGAACTTC CACCAGTGAT GCTGGAACAA AAGGGCCTCC TAGAAGTGAT GGATCAGTTA
    AAACGTTCAC CTTCAGGGTT GGACTCTGTA TTGCAAAAAA CTGTGCCATG GGGAGTAGCA
    TTTCATCATG CAGGTCTTAC TTTTGAGGAG AGAGATATCA TTGAAGGAGC CTTCCGTCAA
    GGTCAGATTC GAGTCTTGGC AGCAACTTCT ACTCTGTCAT CTGGTGTGAA TTTACCTGCA
    CGTCGTGTAA TCATTCGCAC TCCTATTTTC AGTGGTAGAC TTCTAGATAT TCTTACTTAT
    AAGCAGATGG TTGGTCGTGC TGGCAGGAAA GGTGTAGACA CAGTAGGTGA GAGTATCTTA
    GTTTGTAAAA ACTCTGAGAA ATCAAAAGGC ATAACTCTAC TTCAGGGATC TCTGAAACCT
    GTTCATAGCT GTTTGCAAAG ACACGAAGGA GAAGAAGTAA CCTCAAGCAT GATAAGAGCT
    ATTTTGGAGA TAATAGTTGG TGGAGTGGCA AGTACGTCAC AAGATATACA GACTTATGCT
    TCTTGCACAT TTTTGGCTGC AAGTATGAAA GAAGGGAAGC AGGGGATTCA GAAAAATAAA
    GATTCTGTTC AACTTGGAGC AATTGAGGCC TGTGTAATGT GGCTACTAGA AAATGAATTC
    ATCCAGATTA CTGAAGCTAG TGATGGAACA GAAGGAAATG TGTATCATCC AACACACCTT
    GGTTCCGCAA CTCTTTCTTC TTCACTGTCT CCAATTGATA CTTTAGATAT TTTTGCTGAC
    CTACAAAGAG CAATGAAGGG CTTTGTTTTA GAGAATGATC TGCATATTGT TTATTTGGTT
    ACACCTGTGT TTGAGGATTG GACTACTATT GATTGGTATC GATTTTTCTG TTTATGGGAG
    AAGTTGCCAG CTTCTATGAA GCGGGTGGCA GAGCTAGTAG GAGTTGAAGA AGGGTTCTTG
    GCTCGCTGTG TGAAAGGAAA TTTAGTAGCC AGAACTGAGA GACAGCACCG ACAAATGGCC
    ATCCATAAAA GGTTTTTTAC CAGTCTTGTG CTATTAGATT TAATCAGTGA AGTTCCCTTA
    AAGGAAATTA ATCAGAAATA TGGATGCAAT CGTGGACAGA TTCAGTCATT ACAACAGTCA
    GCTGCTGTTT ATGCAGGGAT GATTACAGTG TTTTCCAACC GCCTGGGCTG GCACAACATG
    GAACTACTGC TTTCCCAATT CCAGAAGCGT CTTACATTTG GCATCCAAAG GGAGCTGTGT
    GACCTGGTCC GGGTGTCATT ACTTAATGCA CAGCGGGCCA GATTCCTCTA TGCATCTGGT
    TTTCTTACTG TGTCTGATCT TGCTAGAGCA GATGTTGCTG AGGTGGAAAG TGTTCTGAAA
    AATGCTGCAC CTTTTAAAAG TACTCGAAAG GCAGTGGATG AGGAAGAGGA AGCAGTTGAA
    GAGCGTCGAA ATATGCGAAC TATTTGGGTG ACAGGCAGAA AAGGTTTAAG TGAAAGGGAA
    GCAGCAACCC TTATAGTAGA AGAAGCCAGA ATGATTCTGC AGCAGGACTT AGTTGAAATG
    GGAGTGCAAT GGAATCTACA TTCCTTTCTA AATTCTGATA CACACTTACT GACCAGCAGT
    GAGTCAGAAG TAAAAGAACA CAAATTTATA CCTCAAACTA AGAGTTCTTA TAAAAGATTA
    ACATCAAAGA AAAAAAGTGA CACAATATTT AGTGATTCTG TTAATCACTT ACCAGATACC
    ATGCAAGATT TAGATAAAAG TAGAGAACAT ACAGATTCCT TTTATTATAA ATTTCAGGAT
    GGGAATCAAG AGTGTCATCA TTCCATCTCC AGAGGGAAAA AACAGGCTTC TTTGAGTATT
    AAAAAAGGAA AGCTAGGAAC TTCTCTGAAT GAGGGGAAAG CAAGCACTTC GAAAGTTGTT
    CGGACTCTCT CATCACGGAA AACAAAAAGA GAAGCTTTAA ATTTTAGTTC AGAAAAAGTG
    AGTACAATCT CTCAATCTTG GAAACATGGT AAGCATCGAA AACGATCCAG CGACAGTAGC
    CCTGTGAAAG ACACAAAGAT GCGTAGAATC AATTTACAAG AACGGACTAT GTCTAATTCT
    ATTCATTGTG AAGACTCTTT TACTTTAGAT AAGATGAGTA TGGAATTTAG AAGTCCAGAG
    CCATTTGCTA AAAATGTATC TTTCCGTGCT GAGAGAAGAT GTAACATAAT ACCATTTGCA
    TCACAAACAG AACAAAGTTG TTCTAGGAAC AATAAAATAC CTAATGATAT TCTTGGGGAA
    CACTTGATCA CAGGATCTCA GAGTAAACAT GTGATTCAAG CCACTAGTGT GGTCAGTGAA
    AATGGAAGAG AATTAGCTAT TGAATCAGAA AAAAAAAATA AAGTGGTGAT ACAAAATGAT
    TCAAAAATCC AGAATGTTTA TGTGAAACAC CATGACACCC ATCCAATTAA CCAGTGCCTG
    GAAAAGCAGT TTAATAAAGA GACAAATACT CACATCAAAC TGAATACAAC AGTAGCAAAA
    CAAATACCCT ATGAGACAGA CAGTAGTTAT TTGAATAGAG ACTCAAATGT TATTATGAAA
    TGTGAAAGTA CAAAGTTTAA TGTTGAGGAA AATAAATCAA GTAATCTTAA GGCATCAGGA
    GATATCATAA GCAGCATTGA GATACCCAGT GAAGTACATC TACCAAGTGG AGCATTTGGT
    AAATTAAGAG GCCAGCCTGA GAATATTCTA AATACTCCTA GAGTACAAGG AAGAACAGAT
    GCTTATGCAG CAGACAGAAC TAAAAATAAT TGTGTTTCTG ACTTAGGTTT AGATCTCTGT
    GATCTTGAAG ATAGTTTCTA CCTGGATACT CAGTCAGAGA AAATAATTCA GCAGATAGCA
    ACTGCAAATG CCAACCAAGA AGGACCCAAG GATACCAGCC TGGCAACAGG GATGATTCAG
    AAGAACCTAG ACAAACAGAG CTCAATGAGC TCCTTTCAGA AAGAGCCTCA TGTTGCTTTT
    CCAGGCGAAC AGCATTCACT AGAAATGACA AATCATTTGA AACATAAGAC TGTAGATGTT
    GTGAAGCAAA GCACTGATTC ACATGAGGTT GATACCCTGA CTCCAGAAAG GCCAATTTTT
    TATTCTCCAG TACTAGTAGG GGAAAATGGC CCATGTTTTA AAGGCAGTGA ACTTTCTGTT
    ACTGACTCCC AATTAAATAG TTTTCTTCAA GGTTATCAAA CACAAGAAAC TATGAAGCCA
    GTTATACCTG TGATTCCTCA AAAGAGAATT CCTACTGATA CAGAAGTAGA GTGTCTGCCA
    GTTCCTGAAA CAAGTTTGAA TTTGAGTGGT AGTTTACTGT TTGACAGTTT CAGTGAAGAC
    TACCTTGTAG AAGAACAACG ACCTGATGGA CAAGAAAAAG AACACCTTCC TTCTGAAATA
    ACATCAGTCC ATTTCGATGA TTCTCTGTGT CTACAACCAG AGGGCATAAT TAAAAAACCA
    GATATGAATG AGAATCAAGA TAATCAGCAG CCATTGTCTT GTTTCAGTGA TGAGTCTATT
    ATATTTTCAG AAATCGATTC TGTCCAGATG GTTGCAGCTT TGGACAATGT AGACATAGTT
    CCTGTGCAGG AGAAACATAA TACTGCAGAA TCTCTAAAAA TACTGAAACG GAGTGATCAA
    GTGACTGTAG GTAATGATGA TTCCCAAAGA GAAGTAATTG GAGAAGAAAA AGATAAAAGG
    TCTCAAAAAT CCAAGTTAAC TGGCACAAGG CAAGATAATT CATTTCTATG GTCAGGAGCA
    TCATTTGATT TAAGTCCAGG ACTGCAAAGG ATTTTAGACC AAGTGTCCAG TCCTCTAGAA
    AATGAAAAGC CAAAATTAAC TGCTACAGAC TTGCCTGGTT TGAATGGAGA AAATGCAGAG
    GTAAATGACA GACAAGTGAT TTCAAATACG GAGCCAAATC AAGTACAAGG AATTCCATTT
    TCACCTAAAA TTGTAGTAAG AGGCAATCTT GAGGCACTAC AGAACGATGT CATTCATGGT
    GAAACCTCCC TCTTGCCTTG TAAAGAAAAC TGTATAGTTC GTGATAATGG ACTCATTCCT
    CCTACACCTA TTCCTTCTGA TTCTAAGTTG GCATTTCCAG ATATTCTTGG AACATCTGTG
    AAACTTAAGA AAATTAGTCA TGGTTTTAAA CTTGGTGAAA GTTCTTCATT TTGCTCACCT
    TCCAATAATA AAAACCATGA TTTACATCCA GAGAGTAGAA ATGGTTTTAA AGATAACAGT
    TCTATTAGAG ACACAAATTT TTCACCGCAG TTACTGCAGA ATGGATCACA GTTAACTCTA
    GCTTCATGCA GTCCTGAAAG TTTGGCCATA ATTGATGTAG CAAGTAACCA AACTCTCTTT
    CAAACATTCA TGAAGGAATG GCAAAGCAAA ACTCGATTCT CCATCTCACT GGCTTGTGAA
    AAGATCAGTA ATTTGACATC TTCTAAAAGT GCTACTATTG GTGGTATGTT TAAGCAAGTT
    AGTTTCCCTC AAGAAGCCTC TATTAGAGAT GATGGATTTC CTGTTAAAGA CTGTGATGAC
    ATCTTACTGG TTGGGCTTGC AGTATGTTGG GGTGGAAAAG ATGCTTACTA TTTTTCACTG
    CAGAAGGAAC AAAAGCATTC TGAAATTAGT GCCAGTTTGA TCCCACCTTC TCTGGATCCA
    AGCCTGACTG TGAAGGATAG ATTACGGCAC CTTCAATCTT GCTTTCACAA AGAATCTGAT
    AAAGAACGTT CTGTTATCAT CTATGACTTC ATCCAGAGTT ATAAAATTCT TCTTCTGTCT
    TGTGGCATCT CCCTGGAACA AAGTTACGAA GATCCTAAGG TGGCATGCTG GTTACTAGAT
    CCAGATTCCA AGGAGCCGAC TCTTCACAGC ATAGTTTCCC ATTTTCTTCC TCATGAGCTT
    CCACTCCTGG AAGAAATAGA GACTGGCCAA GGAATTCAAA GTCTAGGGCT GAATGTCAGC
    ACTACGCATT CTGGACGATA CAGAGCATCT GTAGAGTCCA TTCTCATCTT CAACTCTATG
    AATCAACTTA ATTCTTTGTT GAAGAAGGAA AACCTTCAAG ATGTTTTCTA TAAAGTGGAA
    ATGCCCTCCC AGTACTGCCT AGCCTTGCTG GAACTAAATG GAATCGGCTT TAGTACTGCA
    GAATGTGAAA GTCAGAAACA CATAATGCAA GCCAAACTGG ATGCAATTGA GATGCAGGCC
    TATCAACTAG CTGGCCATAG TTTTTCCTTC ACCAGTTCAG ATGACATTGC TGAGGTTTTA
    TTTTTGGAAC TAAAGTTGCC ACCAAATGGA GAGGGAAAAA ACCAAGGCAT CAAGAAAACT
    TTGGGTTCTA CCAGAAGAGG AAATGACAAT GGACGGAAGC TAAGGCTGGG AAAACAGTTC
    AGCACTAGTA AGGGTGTTTT AAATAAATTA AGGGCATTAC ATCCTTTACC AGGCTTGATC
    TTAGAGTGGA GAAGAATCAC TAATGCTATT ACCAAAGTGG TCTTTCCCCT GCAGCGGGAA
    AAACGTCTGA ATCCTTTTCT TGGAATGGAA AGAATCTATC CTGTATCACA ATCACACACT
    GCTACAGGAC GAATAACCTT TATAGAGCCA AATATTCAGA ATGTACCAAG AGATTTTGAG
    ATAAAAATGC CAGCAGTAGT AGAGGAAAGC CCACCTTCCC AAGCTCTAGG AAAAGGCCTA
    CTTCCTATGG GCAGAGGAGG AAGAAAGAAG GACTGTGCCA TGAACGCTGG GCGCCAGACA
    AAGGTGGAAG AGAGAGCCTC AGACAGAGGA ATGCCCTTCT CGGTCAGCAT GCGACATGCC
    TTTGTGCCTT TCACAGGTGG TTTAATATTA GCGGCTGATT ATTCTCAACT TGAGCTGAGG
    ATCTTGGCTC ATTTATCCCA CGATCGTCGT CTCATTCAAG TGCTAAACAC TGGAGCTGAT
    GTTTTCAGGA GTATTGCAGC TGAATGGAAG ATGATTGAGC CAGAGTCTGT TGGGGATAAT
    CTGAGGCAAC AAGCAAAGCA GATTTGCTAT GGAATCATTT ATGGAATGGG AGCTAAGTCT
    TTGGGAGAGC AGATGGGTAT TAAAGAAAAC GATGCTGCTT GCTACATTGA CTCCTTCAAA
    TCCAGATACA CGGGGATTAG TCATTTCATG AGAGAGACCG TGAAGAACTG TAAAAGAGAT
    GGATTTGTTC AGACCATTTT GGGAAGGCGT AGATATTTAC CAGGAATCAA AGACAACAAC
    CCTTATCATA AAGCTCATGC TGAGCGTCAG GCTATCAACA CAAGAGTCCA AGGTTCGGCG
    GCTGATATTG TCAAAATAGC CACAGTTAAT ATTCAGAAGC AGTTAGAGAC CTTCCACTCA
    ACTTTCAAAT CCCATGGTCA TCGAGAGGGT TTGCTCCAAA GTGAAAGAAC AGGATTGTTG
    CCAAAGAAAA AACTGCAAGG GATGTTCTGC CCCATCAGAG GAGGCTTCTT TATCCTTCAG
    CTTCATGATG AACTCTTATA TGAAGTGGCA GAAGAAGACG TTGTTCAGAA CTGGAATTAT
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53127
    Clone ID Related Accession (Same CDS sequence) XM_010824626.2 , XM_010801195.3 , XM_010801195.2
    Accession Version XM_010824626.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822928.1
    CDS362..7996
    Translation

    Target ORF information:

    RefSeq Version XM_010824626.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824626.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53127
    Clone ID Related Accession (Same CDS sequence) XM_010824626.2 , XM_010801195.3 , XM_010801195.2
    Accession Version XM_010801195.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
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    3721
    3781
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799497.1
    CDS425..8059
    Translation

    Target ORF information:

    RefSeq Version XM_010801195.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801195.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53127
    Clone ID Related Accession (Same CDS sequence) XM_010824626.2 , XM_010801195.3 , XM_010801195.2
    Accession Version XM_010801195.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799497.1
    CDS362..7996
    Translation

    Target ORF information:

    RefSeq Version XM_010801195.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGAATATAA CGCGCTGGAG TGGGAAACAG CGACGTTCAG CATCAGACTT AAACTCCTCG 
    TCGGGAAGCG GCTTTGACGG CAGTGACAGC CCTTTGCTCC TTTCCGGGCC GGTGCTGAGC
    CCACCTCCGG GCCTGGGACG CAGCTTGAAG GATCGTGGGC GCCGGCCGGC CGGAGCTACA
    GGGGAATGCA AGCAGACGTT TCCTGATGAT CAAATAGACA AGCTGCTGTT GGCAAATTGG
    GGACTTCCTA CAGCGGTTCT GGAGAAATAT CACAGTTTTG GTGTAAAAAA GATGTTTGAA
    TGGCAGGCAG AGTGCCTTTT GCTTGGACAA GTCCTGGAAG GAAAGAATTT AGTCTATTCA
    GCTCCTACAA GTGCTGGGAA GACTCTGGTT GCTGAGCTAC TTATTTTGAA GCGGGTTTTG
    GAAATGCGGA AGAAAGCTCT TTTTATTCTA CCCTTTGTTT CTGTGGCTAA AGAGAAGAAA
    TACTATCTCC AGAGTCTATT TCAGGAAGTA GGAATAAAAG TAGATGGTTA CATGGGCAGT
    ACCTCCCCAA CAAGGCATTT CTCTTCTTTG GATATTGCTG TCTGCACAAT TGAGAGAGCC
    AATGGTCTGA TCAATCGCCT CATAGAGGAA AATAAGATGG ATCTTTTAGG AATGGTGGTT
    GTGGATGAAT TACATATGTT GGGAGACTCT CACCGAGGGT ATCTACTGGA GCTTTTGCTG
    ACCAAGATTT GCTATATTAC TCAGAAATCA GCATCATGCC AGGCAGATTT AGCCATTCCT
    TTGTCTAATG CTGTGCAGAT TGTTGGCATG AGTGCTACTC TTCCAAATTT GGAGCTTGTG
    GCTTCCTGGT TGAATGCTGA ACTCTACCAC ACTGACTTTC GCCCTGTACC ACTGCTGGAA
    TCCATAAAAA TTGGAAATTC AATTTATGAC TCTTCAATGA AGCTTGTGAG GGAATTTCAA
    CCCATGCTGC AAGTGAAGGG AGATGAGGAT CATGTTGTTA GTTTATGTTA TGAGACTGTT
    CGTGATAACC ATTCAGTATT ACTTTTCTGT CCATCGAAGA AATGGTGTGA GAAGCTGGCA
    GATATCATTG CCCGTGAGTT TTACAGTCTG CACCATCAGG CTGAGGGTCT TACTTTTGAG
    GAGAGAGATA TCATTGAAGG AGCCTTCCGT CAAGGTCAGA TTCGAGTCTT GGCAGCAACT
    TCTACTCTGT CATCTGGTGT GAATTTACCT GCACGTCGTG TAATCATTCG CACTCCTATT
    TTCAGTGGTA GACTTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGTCG TGCTGGCAGG
    AAAGGTGTAG ACACAGTAGG TGAGAGTATC TTAGTTTGTA AAAACTCTGA GAAATCAAAA
    GGCATAACTC TACTTCAGGG ATCTCTGAAA CCTGTTCATA GCTGTTTGCA AAGACACGAA
    GGAGAAGAAG TAACCTCAAG CATGATAAGA GCTATTTTGG AGATAATAGT TGGTGGAGTG
    GCAAGTACGT CACAAGATAT ACAGACTTAT GCTTCTTGCA CATTTTTGGC TGCAAGTATG
    AAAGAAGGGA AGCAGGGGAT TCAGAAAAAT AAAGATTCTG TTCAACTTGG AGCAATTGAG
    GCCTGTGTAA TGTGGCTACT AGAAAATGAA TTCATCCAGA TTACTGAAGC TAGTGATGGA
    ACAGAAGGAA ATGTGTATCA TCCAACACAC CTTGGTTCCG CAACTCTTTC TTCTTCACTG
    TCTCCAATTG ATACTTTAGA TATTTTTGCT GACCTACAAA GAGCAATGAA GGGCTTTGTT
    TTAGAGAATG ATCTGCATAT TGTTTATTTG GTTACACCTG TGTTTGAGGA TTGGACTACT
    ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAGCTTCTAT GAAGCGGGTG
    GCAGAGCTAG TAGGAGTTGA AGAAGGGTTC TTGGCTCGCT GTGTGAAAGG AAATTTAGTA
    GCCAGAACTG AGAGACAGCA CCGACAAATG GCCATCCATA AAAGGTTTTT TACCAGTCTT
    GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAAGGAAA TTAATCAGAA ATATGGATGC
    AATCGTGGAC AGATTCAGTC ATTACAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA
    GTGTTTTCCA ACCGCCTGGG CTGGCACAAC ATGGAACTAC TGCTTTCCCA ATTCCAGAAG
    CGTCTTACAT TTGGCATCCA AAGGGAGCTG TGTGACCTGG TCCGGGTGTC ATTACTTAAT
    GCACAGCGGG CCAGATTCCT CTATGCATCT GGTTTTCTTA CTGTGTCTGA TCTTGCTAGA
    GCAGATGTTG CTGAGGTGGA AAGTGTTCTG AAAAATGCTG CACCTTTTAA AAGTACTCGA
    AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAGCGTC GAAATATGCG AACTATTTGG
    GTGACAGGCA GAAAAGGTTT AAGTGAAAGG GAAGCAGCAA CCCTTATAGT AGAAGAAGCC
    AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCT ACATTCCTTT
    CTAAATTCTG ATACACACTT ACTGACCAGC AGTGAGTCAG AAGTAAAAGA ACACAAATTT
    ATACCTCAAA CTAAGAGTTC TTATAAAAGA TTAACATCAA AGAAAAAAAG TGACACAATA
    TTTAGTGATT CTGTTAATCA CTTACCAGAT ACCATGCAAG ATTTAGATAA AAGTAGAGAA
    CATACAGATT CCTTTTATTA TAAATTTCAG GATGGGAATC AAGAGTGTCA TCATTCCATC
    TCCAGAGGGA AAAAACAGGC TTCTTTGAGT ATTAAAAAAG GAAAGCTAGG AACTTCTCTG
    AATGAGGGGA AAGCAAGCAC TTCGAAAGTT GTTCGGACTC TCTCATCACG GAAAACAAAA
    AGAGAAGCTT TAAATTTTAG TTCAGAAAAA GTGAGTACAA TCTCTCAATC TTGGAAACAT
    GGTAAGCATC GAAAACGATC CAGCGACAGT AGCCCTGTGA AAGACACAAA GATGCGTAGA
    ATCAATTTAC AAGAACGGAC TATGTCTAAT TCTATTCATT GTGAAGACTC TTTTACTTTA
    GATAAGATGA GTATGGAATT TAGAAGTCCA GAGCCATTTG CTAAAAATGT ATCTTTCCGT
    GCTGAGAGAA GATGTAACAT AATACCATTT GCATCACAAA CAGAACAAAG TTGTTCTAGG
    AACAATAAAA TACCTAATGA TATTCTTGGG GAACACTTGA TCACAGGATC TCAGAGTAAA
    CATGTGATTC AAGCCACTAG TGTGGTCAGT GAAAATGGAA GAGAATTAGC TATTGAATCA
    GAAAAAAAAA ATAAAGTGGT GATACAAAAT GATTCAAAAA TCCAGAATGT TTATGTGAAA
    CACCATGACA CCCATCCAAT TAACCAGTGC CTGGAAAAGC AGTTTAATAA AGAGACAAAT
    ACTCACATCA AACTGAATAC AACAGTAGCA AAACAAATAC CCTATGAGAC AGACAGTAGT
    TATTTGAATA GAGACTCAAA TGTTATTATG AAATGTGAAA GTACAAAGTT TAATGTTGAG
    GAAAATAAAT CAAGTAATCT TAAGGCATCA GGAGATATCA TAAGCAGCAT TGAGATACCC
    AGTGAAGTAC ATCTACCAAG TGGAGCATTT GGTAAATTAA GAGGCCAGCC TGAGAATATT
    CTAAATACTC CTAGAGTACA AGGAAGAACA GATGCTTATG CAGCAGACAG AACTAAAAAT
    AATTGTGTTT CTGACTTAGG TTTAGATCTC TGTGATCTTG AAGATAGTTT CTACCTGGAT
    ACTCAGTCAG AGAAAATAAT TCAGCAGATA GCAACTGCAA ATGCCAACCA AGAAGGACCC
    AAGGATACCA GCCTGGCAAC AGGGATGATT CAGAAGAACC TAGACAAACA GAGCTCAATG
    AGCTCCTTTC AGAAAGAGCC TCATGTTGCT TTTCCAGGCG AACAGCATTC ACTAGAAATG
    ACAAATCATT TGAAACATAA GACTGTAGAT GTTGTGAAGC AAAGCACTGA TTCACATGAG
    GTTGATACCC TGACTCCAGA AAGGCCAATT TTTTATTCTC CAGTACTAGT AGGGGAAAAT
    GGCCCATGTT TTAAAGGCAG TGAACTTTCT GTTACTGACT CCCAATTAAA TAGTTTTCTT
    CAAGGTTATC AAACACAAGA AACTATGAAG CCAGTTATAC CTGTGATTCC TCAAAAGAGA
    ATTCCTACTG ATACAGAAGT AGAGTGTCTG CCAGTTCCTG AAACAAGTTT GAATTTGAGT
    GGTAGTTTAC TGTTTGACAG TTTCAGTGAA GACTACCTTG TAGAAGAACA ACGACCTGAT
    GGACAAGAAA AAGAACACCT TCCTTCTGAA ATAACATCAG TCCATTTCGA TGATTCTCTG
    TGTCTACAAC CAGAGGGCAT AATTAAAAAA CCAGATATGA ATGAGAATCA AGATAATCAG
    CAGCCATTGT CTTGTTTCAG TGATGAGTCT ATTATATTTT CAGAAATCGA TTCTGTCCAG
    ATGGTTGCAG CTTTGGACAA TGTAGACATA GTTCCTGTGC AGGAGAAACA TAATACTGCA
    GAATCTCTAA AAATACTGAA ACGGAGTGAT CAAGTGACTG TAGGTAATGA TGATTCCCAA
    AGAGAAGTAA TTGGAGAAGA AAAAGATAAA AGGTCTCAAA AATCCAAGTT AACTGGCACA
    AGGCAAGATA ATTCATTTCT ATGGTCAGGA GCATCATTTG ATTTAAGTCC AGGACTGCAA
    AGGATTTTAG ACCAAGTGTC CAGTCCTCTA GAAAATGAAA AGCCAAAATT AACTGCTACA
    GACTTGCCTG GTTTGAATGG AGAAAATGCA GAGGTAAATG ACAGACAAGT GATTTCAAAT
    ACGGAGCCAA ATCAAGTACA AGGAATTCCA TTTTCACCTA AAATTGTAGT AAGAGGCAAT
    CTTGAGGCAC TACAGAACGA TGTCATTCAT GGTGAAACCT CCCTCTTGCC TTGTAAAGAA
    AACTGTATAG TTCGTGATAA TGGACTCATT CCTCCTACAC CTATTCCTTC TGATTCTAAG
    TTGGCATTTC CAGATATTCT TGGAACATCT GTGAAACTTA AGAAAATTAG TCATGGTTTT
    AAACTTGGTG AAAGTTCTTC ATTTTGCTCA CCTTCCAATA ATAAAAACCA TGATTTACAT
    CCAGAGAGTA GAAATGGTTT TAAAGATAAC AGTTCTATTA GAGACACAAA TTTTTCACCG
    CAGTTACTGC AGAATGGATC ACAGTTAACT CTAGCTTCAT GCAGTCCTGA AAGTTTGGCC
    ATAATTGATG TAGCAAGTAA CCAAACTCTC TTTCAAACAT TCATGAAGGA ATGGCAAAGC
    AAAACTCGAT TCTCCATCTC ACTGGCTTGT GAAAAGATCA GTAATTTGAC ATCTTCTAAA
    AGTGCTACTA TTGGTGGTAT GTTTAAGCAA GTTAGTTTCC CTCAAGAAGC CTCTATTAGA
    GATGATGGAT TTCCTGTTAA AGACTGTGAT GACATCTTAC TGGTTGGGCT TGCAGTATGT
    TGGGGTGGAA AAGATGCTTA CTATTTTTCA CTGCAGAAGG AACAAAAGCA TTCTGAAATT
    AGTGCCAGTT TGATCCCACC TTCTCTGGAT CCAAGCCTGA CTGTGAAGGA TAGATTACGG
    CACCTTCAAT CTTGCTTTCA CAAAGAATCT GATAAAGAAC GTTCTGTTAT CATCTATGAC
    TTCATCCAGA GTTATAAAAT TCTTCTTCTG TCTTGTGGCA TCTCCCTGGA ACAAAGTTAC
    GAAGATCCTA AGGTGGCATG CTGGTTACTA GATCCAGATT CCAAGGAGCC GACTCTTCAC
    AGCATAGTTT CCCATTTTCT TCCTCATGAG CTTCCACTCC TGGAAGAAAT AGAGACTGGC
    CAAGGAATTC AAAGTCTAGG GCTGAATGTC AGCACTACGC ATTCTGGACG ATACAGAGCA
    TCTGTAGAGT CCATTCTCAT CTTCAACTCT ATGAATCAAC TTAATTCTTT GTTGAAGAAG
    GAAAACCTTC AAGATGTTTT CTATAAAGTG GAAATGCCCT CCCAGTACTG CCTAGCCTTG
    CTGGAACTAA ATGGAATCGG CTTTAGTACT GCAGAATGTG AAAGTCAGAA ACACATAATG
    CAAGCCAAAC TGGATGCAAT TGAGATGCAG GCCTATCAAC TAGCTGGCCA TAGTTTTTCC
    TTCACCAGTT CAGATGACAT TGCTGAGGTT TTATTTTTGG AACTAAAGTT GCCACCAAAT
    GGAGAGGGAA AAAACCAAGG CATCAAGAAA ACTTTGGGTT CTACCAGAAG AGGAAATGAC
    AATGGACGGA AGCTAAGGCT GGGAAAACAG TTCAGCACTA GTAAGGGTGT TTTAAATAAA
    TTAAGGGCAT TACATCCTTT ACCAGGCTTG ATCTTAGAGT GGAGAAGAAT CACTAATGCT
    ATTACCAAAG TGGTCTTTCC CCTGCAGCGG GAAAAACGTC TGAATCCTTT TCTTGGAATG
    GAAAGAATCT ATCCTGTATC ACAATCACAC ACTGCTACAG GACGAATAAC CTTTATAGAG
    CCAAATATTC AGAATGTACC AAGAGATTTT GAGATAAAAA TGCCAGCAGT AGTAGAGGAA
    AGCCCACCTT CCCAAGCTCT AGGAAAAGGC CTACTTCCTA TGGGCAGAGG AGGAAGAAAG
    AAGGACTGTG CCATGAACGC TGGGCGCCAG ACAAAGGTGG AAGAGAGAGC CTCAGACAGA
    GGAATGCCCT TCTCGGTCAG CATGCGACAT GCCTTTGTGC CTTTCACAGG TGGTTTAATA
    TTAGCGGCTG ATTATTCTCA ACTTGAGCTG AGGATCTTGG CTCATTTATC CCACGATCGT
    CGTCTCATTC AAGTGCTAAA CACTGGAGCT GATGTTTTCA GGAGTATTGC AGCTGAATGG
    AAGATGATTG AGCCAGAGTC TGTTGGGGAT AATCTGAGGC AACAAGCAAA GCAGATTTGC
    TATGGAATCA TTTATGGAAT GGGAGCTAAG TCTTTGGGAG AGCAGATGGG TATTAAAGAA
    AACGATGCTG CTTGCTACAT TGACTCCTTC AAATCCAGAT ACACGGGGAT TAGTCATTTC
    ATGAGAGAGA CCGTGAAGAA CTGTAAAAGA GATGGATTTG TTCAGACCAT TTTGGGAAGG
    CGTAGATATT TACCAGGAAT CAAAGACAAC AACCCTTATC ATAAAGCTCA TGCTGAGCGT
    CAGGCTATCA ACACAAGAGT CCAAGGTTCG GCGGCTGATA TTGTCAAAAT AGCCACAGTT
    AATATTCAGA AGCAGTTAGA GACCTTCCAC TCAACTTTCA AATCCCATGG TCATCGAGAG
    GGTTTGCTCC AAAGTGAAAG AACAGGATTG TTGCCAAAGA AAAAACTGCA AGGGATGTTC
    TGCCCCATCA GAGGAGGCTT CTTTATCCTT CAGCTTCATG ATGAACTCTT ATATGAAGTG
    GCAGAAGAAG ACGTTGTTCA GGTAGCTCAG ATTGTCAAGA ATGAAATGGA AAGTGCCATA
    AAACTGTCTG TGAAACTGAG AGTGAAAGTG AGGATAGGTG CCAGCTGGGG GGAGCTGAAG
    GACTTTGACG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53128
    Clone ID Related Accession (Same CDS sequence) XM_010801196.2 , XM_010824627.2 , XM_010801196.3
    Accession Version XM_010801196.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799498.1
    CDS407..7657
    Translation

    Target ORF information:

    RefSeq Version XM_010801196.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801196.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53128
    Clone ID Related Accession (Same CDS sequence) XM_010801196.2 , XM_010824627.2 , XM_010801196.3
    Accession Version XM_010824627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822929.1
    CDS407..7657
    Translation

    Target ORF information:

    RefSeq Version XM_010824627.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), theta (POLQ), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53128
    Clone ID Related Accession (Same CDS sequence) XM_010801196.2 , XM_010824627.2 , XM_010801196.3
    Accession Version XM_010801196.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase theta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799498.1
    CDS243..7493
    Translation

    Target ORF information:

    RefSeq Version XM_010801196.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase theta (POLQ), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801196.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGGCAGTA CCTCCCCAAC AAGGCATTTC TCTTCTTTGG ATATTGCTGT CTGCACAATT 
    GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTTTTAGGA
    ATGGTGGTTG TGGATGAATT ACATATGTTG GGAGACTCTC ACCGAGGGTA TCTACTGGAG
    CTTTTGCTGA CCAAGATTTG CTATATTACT CAGAAATCAG CATCATGCCA GGCAGATTTA
    GCCATTCCTT TGTCTAATGC TGTGCAGATT GTTGGCATGA GTGCTACTCT TCCAAATTTG
    GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCACA CTGACTTTCG CCCTGTACCA
    CTGCTGGAAT CCATAAAAAT TGGAAATTCA ATTTATGACT CTTCAATGAA GCTTGTGAGG
    GAATTTCAAC CCATGCTGCA AGTGAAGGGA GATGAGGATC ATGTTGTTAG TTTATGTTAT
    GAGACTGTTC GTGATAACCA TTCAGTATTA CTTTTCTGTC CATCGAAGAA ATGGTGTGAG
    AAGCTGGCAG ATATCATTGC CCGTGAGTTT TACAGTCTGC ACCATCAGGC TGAGGGATTG
    GTAAGATCAT CTGAACTTCC ACCAGTGATG CTGGAACAAA AGGGCCTCCT AGAAGTGATG
    GATCAGTTAA AACGTTCACC TTCAGGGTTG GACTCTGTAT TGCAAAAAAC TGTGCCATGG
    GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GAGATATCAT TGAAGGAGCC
    TTCCGTCAAG GTCAGATTCG AGTCTTGGCA GCAACTTCTA CTCTGTCATC TGGTGTGAAT
    TTACCTGCAC GTCGTGTAAT CATTCGCACT CCTATTTTCA GTGGTAGACT TCTAGATATT
    CTTACTTATA AGCAGATGGT TGGTCGTGCT GGCAGGAAAG GTGTAGACAC AGTAGGTGAG
    AGTATCTTAG TTTGTAAAAA CTCTGAGAAA TCAAAAGGCA TAACTCTACT TCAGGGATCT
    CTGAAACCTG TTCATAGCTG TTTGCAAAGA CACGAAGGAG AAGAAGTAAC CTCAAGCATG
    ATAAGAGCTA TTTTGGAGAT AATAGTTGGT GGAGTGGCAA GTACGTCACA AGATATACAG
    ACTTATGCTT CTTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA GGGGATTCAG
    AAAAATAAAG ATTCTGTTCA ACTTGGAGCA ATTGAGGCCT GTGTAATGTG GCTACTAGAA
    AATGAATTCA TCCAGATTAC TGAAGCTAGT GATGGAACAG AAGGAAATGT GTATCATCCA
    ACACACCTTG GTTCCGCAAC TCTTTCTTCT TCACTGTCTC CAATTGATAC TTTAGATATT
    TTTGCTGACC TACAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT GCATATTGTT
    TATTTGGTTA CACCTGTGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT
    TTATGGGAGA AGTTGCCAGC TTCTATGAAG CGGGTGGCAG AGCTAGTAGG AGTTGAAGAA
    GGGTTCTTGG CTCGCTGTGT GAAAGGAAAT TTAGTAGCCA GAACTGAGAG ACAGCACCGA
    CAAATGGCCA TCCATAAAAG GTTTTTTACC AGTCTTGTGC TATTAGATTT AATCAGTGAA
    GTTCCCTTAA AGGAAATTAA TCAGAAATAT GGATGCAATC GTGGACAGAT TCAGTCATTA
    CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTGT TTTCCAACCG CCTGGGCTGG
    CACAACATGG AACTACTGCT TTCCCAATTC CAGAAGCGTC TTACATTTGG CATCCAAAGG
    GAGCTGTGTG ACCTGGTCCG GGTGTCATTA CTTAATGCAC AGCGGGCCAG ATTCCTCTAT
    GCATCTGGTT TTCTTACTGT GTCTGATCTT GCTAGAGCAG ATGTTGCTGA GGTGGAAAGT
    GTTCTGAAAA ATGCTGCACC TTTTAAAAGT ACTCGAAAGG CAGTGGATGA GGAAGAGGAA
    GCAGTTGAAG AGCGTCGAAA TATGCGAACT ATTTGGGTGA CAGGCAGAAA AGGTTTAAGT
    GAAAGGGAAG CAGCAACCCT TATAGTAGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA
    GTTGAAATGG GAGTGCAATG GAATCTACAT TCCTTTCTAA ATTCTGATAC ACACTTACTG
    ACCAGCAGTG AGTCAGAAGT AAAAGAACAC AAATTTATAC CTCAAACTAA GAGTTCTTAT
    AAAAGATTAA CATCAAAGAA AAAAAGTGAC ACAATATTTA GTGATTCTGT TAATCACTTA
    CCAGATACCA TGCAAGATTT AGATAAAAGT AGAGAACATA CAGATTCCTT TTATTATAAA
    TTTCAGGATG GGAATCAAGA GTGTCATCAT TCCATCTCCA GAGGGAAAAA ACAGGCTTCT
    TTGAGTATTA AAAAAGGAAA GCTAGGAACT TCTCTGAATG AGGGGAAAGC AAGCACTTCG
    AAAGTTGTTC GGACTCTCTC ATCACGGAAA ACAAAAAGAG AAGCTTTAAA TTTTAGTTCA
    GAAAAAGTGA GTACAATCTC TCAATCTTGG AAACATGGTA AGCATCGAAA ACGATCCAGC
    GACAGTAGCC CTGTGAAAGA CACAAAGATG CGTAGAATCA ATTTACAAGA ACGGACTATG
    TCTAATTCTA TTCATTGTGA AGACTCTTTT ACTTTAGATA AGATGAGTAT GGAATTTAGA
    AGTCCAGAGC CATTTGCTAA AAATGTATCT TTCCGTGCTG AGAGAAGATG TAACATAATA
    CCATTTGCAT CACAAACAGA ACAAAGTTGT TCTAGGAACA ATAAAATACC TAATGATATT
    CTTGGGGAAC ACTTGATCAC AGGATCTCAG AGTAAACATG TGATTCAAGC CACTAGTGTG
    GTCAGTGAAA ATGGAAGAGA ATTAGCTATT GAATCAGAAA AAAAAAATAA AGTGGTGATA
    CAAAATGATT CAAAAATCCA GAATGTTTAT GTGAAACACC ATGACACCCA TCCAATTAAC
    CAGTGCCTGG AAAAGCAGTT TAATAAAGAG ACAAATACTC ACATCAAACT GAATACAACA
    GTAGCAAAAC AAATACCCTA TGAGACAGAC AGTAGTTATT TGAATAGAGA CTCAAATGTT
    ATTATGAAAT GTGAAAGTAC AAAGTTTAAT GTTGAGGAAA ATAAATCAAG TAATCTTAAG
    GCATCAGGAG ATATCATAAG CAGCATTGAG ATACCCAGTG AAGTACATCT ACCAAGTGGA
    GCATTTGGTA AATTAAGAGG CCAGCCTGAG AATATTCTAA ATACTCCTAG AGTACAAGGA
    AGAACAGATG CTTATGCAGC AGACAGAACT AAAAATAATT GTGTTTCTGA CTTAGGTTTA
    GATCTCTGTG ATCTTGAAGA TAGTTTCTAC CTGGATACTC AGTCAGAGAA AATAATTCAG
    CAGATAGCAA CTGCAAATGC CAACCAAGAA GGACCCAAGG ATACCAGCCT GGCAACAGGG
    ATGATTCAGA AGAACCTAGA CAAACAGAGC TCAATGAGCT CCTTTCAGAA AGAGCCTCAT
    GTTGCTTTTC CAGGCGAACA GCATTCACTA GAAATGACAA ATCATTTGAA ACATAAGACT
    GTAGATGTTG TGAAGCAAAG CACTGATTCA CATGAGGTTG ATACCCTGAC TCCAGAAAGG
    CCAATTTTTT ATTCTCCAGT ACTAGTAGGG GAAAATGGCC CATGTTTTAA AGGCAGTGAA
    CTTTCTGTTA CTGACTCCCA ATTAAATAGT TTTCTTCAAG GTTATCAAAC ACAAGAAACT
    ATGAAGCCAG TTATACCTGT GATTCCTCAA AAGAGAATTC CTACTGATAC AGAAGTAGAG
    TGTCTGCCAG TTCCTGAAAC AAGTTTGAAT TTGAGTGGTA GTTTACTGTT TGACAGTTTC
    AGTGAAGACT ACCTTGTAGA AGAACAACGA CCTGATGGAC AAGAAAAAGA ACACCTTCCT
    TCTGAAATAA CATCAGTCCA TTTCGATGAT TCTCTGTGTC TACAACCAGA GGGCATAATT
    AAAAAACCAG ATATGAATGA GAATCAAGAT AATCAGCAGC CATTGTCTTG TTTCAGTGAT
    GAGTCTATTA TATTTTCAGA AATCGATTCT GTCCAGATGG TTGCAGCTTT GGACAATGTA
    GACATAGTTC CTGTGCAGGA GAAACATAAT ACTGCAGAAT CTCTAAAAAT ACTGAAACGG
    AGTGATCAAG TGACTGTAGG TAATGATGAT TCCCAAAGAG AAGTAATTGG AGAAGAAAAA
    GATAAAAGGT CTCAAAAATC CAAGTTAACT GGCACAAGGC AAGATAATTC ATTTCTATGG
    TCAGGAGCAT CATTTGATTT AAGTCCAGGA CTGCAAAGGA TTTTAGACCA AGTGTCCAGT
    CCTCTAGAAA ATGAAAAGCC AAAATTAACT GCTACAGACT TGCCTGGTTT GAATGGAGAA
    AATGCAGAGG TAAATGACAG ACAAGTGATT TCAAATACGG AGCCAAATCA AGTACAAGGA
    ATTCCATTTT CACCTAAAAT TGTAGTAAGA GGCAATCTTG AGGCACTACA GAACGATGTC
    ATTCATGGTG AAACCTCCCT CTTGCCTTGT AAAGAAAACT GTATAGTTCG TGATAATGGA
    CTCATTCCTC CTACACCTAT TCCTTCTGAT TCTAAGTTGG CATTTCCAGA TATTCTTGGA
    ACATCTGTGA AACTTAAGAA AATTAGTCAT GGTTTTAAAC TTGGTGAAAG TTCTTCATTT
    TGCTCACCTT CCAATAATAA AAACCATGAT TTACATCCAG AGAGTAGAAA TGGTTTTAAA
    GATAACAGTT CTATTAGAGA CACAAATTTT TCACCGCAGT TACTGCAGAA TGGATCACAG
    TTAACTCTAG CTTCATGCAG TCCTGAAAGT TTGGCCATAA TTGATGTAGC AAGTAACCAA
    ACTCTCTTTC AAACATTCAT GAAGGAATGG CAAAGCAAAA CTCGATTCTC CATCTCACTG
    GCTTGTGAAA AGATCAGTAA TTTGACATCT TCTAAAAGTG CTACTATTGG TGGTATGTTT
    AAGCAAGTTA GTTTCCCTCA AGAAGCCTCT ATTAGAGATG ATGGATTTCC TGTTAAAGAC
    TGTGATGACA TCTTACTGGT TGGGCTTGCA GTATGTTGGG GTGGAAAAGA TGCTTACTAT
    TTTTCACTGC AGAAGGAACA AAAGCATTCT GAAATTAGTG CCAGTTTGAT CCCACCTTCT
    CTGGATCCAA GCCTGACTGT GAAGGATAGA TTACGGCACC TTCAATCTTG CTTTCACAAA
    GAATCTGATA AAGAACGTTC TGTTATCATC TATGACTTCA TCCAGAGTTA TAAAATTCTT
    CTTCTGTCTT GTGGCATCTC CCTGGAACAA AGTTACGAAG ATCCTAAGGT GGCATGCTGG
    TTACTAGATC CAGATTCCAA GGAGCCGACT CTTCACAGCA TAGTTTCCCA TTTTCTTCCT
    CATGAGCTTC CACTCCTGGA AGAAATAGAG ACTGGCCAAG GAATTCAAAG TCTAGGGCTG
    AATGTCAGCA CTACGCATTC TGGACGATAC AGAGCATCTG TAGAGTCCAT TCTCATCTTC
    AACTCTATGA ATCAACTTAA TTCTTTGTTG AAGAAGGAAA ACCTTCAAGA TGTTTTCTAT
    AAAGTGGAAA TGCCCTCCCA GTACTGCCTA GCCTTGCTGG AACTAAATGG AATCGGCTTT
    AGTACTGCAG AATGTGAAAG TCAGAAACAC ATAATGCAAG CCAAACTGGA TGCAATTGAG
    ATGCAGGCCT ATCAACTAGC TGGCCATAGT TTTTCCTTCA CCAGTTCAGA TGACATTGCT
    GAGGTTTTAT TTTTGGAACT AAAGTTGCCA CCAAATGGAG AGGGAAAAAA CCAAGGCATC
    AAGAAAACTT TGGGTTCTAC CAGAAGAGGA AATGACAATG GACGGAAGCT AAGGCTGGGA
    AAACAGTTCA GCACTAGTAA GGGTGTTTTA AATAAATTAA GGGCATTACA TCCTTTACCA
    GGCTTGATCT TAGAGTGGAG AAGAATCACT AATGCTATTA CCAAAGTGGT CTTTCCCCTG
    CAGCGGGAAA AACGTCTGAA TCCTTTTCTT GGAATGGAAA GAATCTATCC TGTATCACAA
    TCACACACTG CTACAGGACG AATAACCTTT ATAGAGCCAA ATATTCAGAA TGTACCAAGA
    GATTTTGAGA TAAAAATGCC AGCAGTAGTA GAGGAAAGCC CACCTTCCCA AGCTCTAGGA
    AAAGGCCTAC TTCCTATGGG CAGAGGAGGA AGAAAGAAGG ACTGTGCCAT GAACGCTGGG
    CGCCAGACAA AGGTGGAAGA GAGAGCCTCA GACAGAGGAA TGCCCTTCTC GGTCAGCATG
    CGACATGCCT TTGTGCCTTT CACAGGTGGT TTAATATTAG CGGCTGATTA TTCTCAACTT
    GAGCTGAGGA TCTTGGCTCA TTTATCCCAC GATCGTCGTC TCATTCAAGT GCTAAACACT
    GGAGCTGATG TTTTCAGGAG TATTGCAGCT GAATGGAAGA TGATTGAGCC AGAGTCTGTT
    GGGGATAATC TGAGGCAACA AGCAAAGCAG ATTTGCTATG GAATCATTTA TGGAATGGGA
    GCTAAGTCTT TGGGAGAGCA GATGGGTATT AAAGAAAACG ATGCTGCTTG CTACATTGAC
    TCCTTCAAAT CCAGATACAC GGGGATTAGT CATTTCATGA GAGAGACCGT GAAGAACTGT
    AAAAGAGATG GATTTGTTCA GACCATTTTG GGAAGGCGTA GATATTTACC AGGAATCAAA
    GACAACAACC CTTATCATAA AGCTCATGCT GAGCGTCAGG CTATCAACAC AAGAGTCCAA
    GGTTCGGCGG CTGATATTGT CAAAATAGCC ACAGTTAATA TTCAGAAGCA GTTAGAGACC
    TTCCACTCAA CTTTCAAATC CCATGGTCAT CGAGAGGGTT TGCTCCAAAG TGAAAGAACA
    GGATTGTTGC CAAAGAAAAA ACTGCAAGGG ATGTTCTGCC CCATCAGAGG AGGCTTCTTT
    ATCCTTCAGC TTCATGATGA ACTCTTATAT GAAGTGGCAG AAGAAGACGT TGTTCAGGTA
    GCTCAGATTG TCAAGAATGA AATGGAAAGT GCCATAAAAC TGTCTGTGAA ACTGAGAGTG
    AAAGTGAGGA TAGGTGCCAG CTGGGGGGAG CTGAAGGACT TTGACGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.