MYO7A cDNA ORF clone, Bos taurus(cattle)

The following MYO7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa92776 XM_015466591.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92776 XM_015474831.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa223305 XM_010812768.3
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223306 XM_015474829.2
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223307 XM_024975887.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa92774 XM_015474829.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa92774 XM_015466589.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa92775 XM_015466590.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa92775 XM_015474830.1
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa53031 XM_010812768.2
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa53031 XM_865073.8
Latest version!
Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa92776
    Clone ID Related Accession (Same CDS sequence) XM_015474831.1 , XM_015466591.1
    Accession Version XM_015474831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGGGATC TTCCTGACCC AGGGATCGAA CCTGCATCTC
    TTGTGTCTCC TGCACTGGCA GATGGATTCT TTACCACTGA GCTACCACAG CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330317.1
    CDS2164..6180
    Translation

    Target ORF information:

    RefSeq Version XM_015474831.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGGGATC TTCCTGACCC AGGGATCGAA CCTGCATCTC
    TTGTGTCTCC TGCACTGGCA GATGGATTCT TTACCACTGA GCTACCACAG CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92776
    Clone ID Related Accession (Same CDS sequence) XM_015474831.1 , XM_015466591.1
    Accession Version XM_015466591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGGGATC TTCCTGACCC AGGGATCGAA CCTGCATCTC
    TTGTGTCTCC TGCACTGGCA GATGGATTCT TTACCACTGA GCTACCACAG CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322077.1
    CDS2164..6180
    Translation

    Target ORF information:

    RefSeq Version XM_015466591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGGGATC TTCCTGACCC AGGGATCGAA CCTGCATCTC
    TTGTGTCTCC TGCACTGGCA GATGGATTCT TTACCACTGA GCTACCACAG CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223305
    Clone ID Related Accession (Same CDS sequence) XM_010812768.3
    Accession Version XM_010812768.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCGGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGACAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGCGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811070.1
    CDS1284..7910
    Translation

    Target ORF information:

    RefSeq Version XM_010812768.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812768.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCGGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGACAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGCGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223306
    Clone ID Related Accession (Same CDS sequence) XM_015474829.2
    Accession Version XM_015474829.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCGGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    CGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330315.1
    CDS1283..7816
    Translation

    Target ORF information:

    RefSeq Version XM_015474829.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474829.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCGGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    CGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223307
    Clone ID Related Accession (Same CDS sequence) XM_024975887.1
    Accession Version XM_024975887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGCCCTGGT CACCATGACC CCCGACCAGA GGCAGGATGT TATCCGACTC
    CTGCAGCTGC GAACGGCAGA GCCCGAGGCG CGCACCAAGC CCTACACACT GGAGGAGTTT
    TCCTACGACT ACTTCAGGCC CCCACCCAAG CACACACTGA GCCGCGTCAT GGTGTCCAAG
    GCCCGCGGCA AGGACCGGCT GTGGAGCCAC ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC
    AAGAAGATCC TGGGCAGCGA GGAGCTCTCA CAGGAGGCCT GCATGGCCTT CACTGCTGTG
    CTCAAGTACA TGGGTGACTA CCCGTCCAAG AGGATGCGCT CCGTCAATGA GCTCACCGAC
    CAGATCTTCG AGGGAGCCCT GAAGGCCGAG CCCCTCAAGG ACGAGGTGTA TGTGCAGATC
    CTGAAGCAGC TGACTGACAA CCACATCAGG TACAGTGAGG AGCGGGGCTG GGAGCTACTC
    TGGTTGTGCA CAGGGCTCTT CCCACCCAGC AACATCCTCC TGCCCCACGT GCAGCGCTTC
    CTGCAGTCCC GCAAACACTG CCCACTTGCC ATCGACTGCC TGCAGCGACT TCAGAAAGCC
    CTGAGAAATG GGTCCCGGAA GTACCCCCCA CACTTGGTGG AGGTGGAAGC CATCCAGCAC
    AAAACCACCC AGATTTTCCA CAAGGTCTAC TTTCCTGATG ACACTGACGA GGCCTTTGAG
    GTGGAGTCCA GCACCAAGGC CAAGGACTTC TGCCAGAATA TCGCCGCCCG GCTGCTCCTC
    AAGTCCTCCG AGGGATTCAG CCTCTTTGTC AAAATTGTAG ATAAGGTCAT CAGCGTCCCT
    GAGAATGACT TCTTCTTCGA CTTTGTACGA CATCTGACGG ACTGGATAAA GAAAGCCCGG
    CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG ACCTACCAAG TGTTCTTCAT GAAGAAGCTG
    TGGACCACCA CAGTGCCGGG GAAGGACCCC ATGGCCGATT CCATCTTCCA CTATTACCAG
    GAGTTGCCCA AGTACCTCCG AGGCTACCAC AAGTGCACGC GGGAAGAGGT GCTGCAGCTG
    GGGGCGCTCA TCTATAGGGT CAAGTTCGAG GAGGACAAGT CCTACTTCCC CAGTATCCCC
    AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC CTCATCCGGC AGGTCTCGCC TGACGACTGG
    AAGCGGTCCA TCGTCGCCTA TTTCAACAAG CACGCGGGGA AGTCCAAGGA GGAGGCCAAG
    CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG CCCACCTTCG GCTCAGCCTT CTTTGAGGTG
    AAGCAAACTA CAGAGCCAAA CTTTCCTGAG ATCCTCTTAA TTGCCATCAA CAAGTATGGG
    GTCAGCCTCA TTGATCCGAG AACCAAGGAC ATCCTGACCA CTCACCCCTT CACCAAGATC
    TCCAACTGGA GCAGCGGCAA CACCTACTTC CACATCACCA TTGGGAACCT GGTGCGTGGG
    AGCAAACTCC TCTGTGAGAC GTCACTGGGC TACAAGATGG ATGACCTCCT GACTTCCTAT
    ATTAGCCAGA TGCTTACGGC CATGAGCAAA CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831655.1
    CDS1283..7699
    Translation

    Target ORF information:

    RefSeq Version XM_024975887.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGCCCTGGT CACCATGACC CCCGACCAGA GGCAGGATGT TATCCGACTC
    CTGCAGCTGC GAACGGCAGA GCCCGAGGCG CGCACCAAGC CCTACACACT GGAGGAGTTT
    TCCTACGACT ACTTCAGGCC CCCACCCAAG CACACACTGA GCCGCGTCAT GGTGTCCAAG
    GCCCGCGGCA AGGACCGGCT GTGGAGCCAC ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC
    AAGAAGATCC TGGGCAGCGA GGAGCTCTCA CAGGAGGCCT GCATGGCCTT CACTGCTGTG
    CTCAAGTACA TGGGTGACTA CCCGTCCAAG AGGATGCGCT CCGTCAATGA GCTCACCGAC
    CAGATCTTCG AGGGAGCCCT GAAGGCCGAG CCCCTCAAGG ACGAGGTGTA TGTGCAGATC
    CTGAAGCAGC TGACTGACAA CCACATCAGG TACAGTGAGG AGCGGGGCTG GGAGCTACTC
    TGGTTGTGCA CAGGGCTCTT CCCACCCAGC AACATCCTCC TGCCCCACGT GCAGCGCTTC
    CTGCAGTCCC GCAAACACTG CCCACTTGCC ATCGACTGCC TGCAGCGACT TCAGAAAGCC
    CTGAGAAATG GGTCCCGGAA GTACCCCCCA CACTTGGTGG AGGTGGAAGC CATCCAGCAC
    AAAACCACCC AGATTTTCCA CAAGGTCTAC TTTCCTGATG ACACTGACGA GGCCTTTGAG
    GTGGAGTCCA GCACCAAGGC CAAGGACTTC TGCCAGAATA TCGCCGCCCG GCTGCTCCTC
    AAGTCCTCCG AGGGATTCAG CCTCTTTGTC AAAATTGTAG ATAAGGTCAT CAGCGTCCCT
    GAGAATGACT TCTTCTTCGA CTTTGTACGA CATCTGACGG ACTGGATAAA GAAAGCCCGG
    CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG ACCTACCAAG TGTTCTTCAT GAAGAAGCTG
    TGGACCACCA CAGTGCCGGG GAAGGACCCC ATGGCCGATT CCATCTTCCA CTATTACCAG
    GAGTTGCCCA AGTACCTCCG AGGCTACCAC AAGTGCACGC GGGAAGAGGT GCTGCAGCTG
    GGGGCGCTCA TCTATAGGGT CAAGTTCGAG GAGGACAAGT CCTACTTCCC CAGTATCCCC
    AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC CTCATCCGGC AGGTCTCGCC TGACGACTGG
    AAGCGGTCCA TCGTCGCCTA TTTCAACAAG CACGCGGGGA AGTCCAAGGA GGAGGCCAAG
    CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG CCCACCTTCG GCTCAGCCTT CTTTGAGGTG
    AAGCAAACTA CAGAGCCAAA CTTTCCTGAG ATCCTCTTAA TTGCCATCAA CAAGTATGGG
    GTCAGCCTCA TTGATCCGAG AACCAAGGAC ATCCTGACCA CTCACCCCTT CACCAAGATC
    TCCAACTGGA GCAGCGGCAA CACCTACTTC CACATCACCA TTGGGAACCT GGTGCGTGGG
    AGCAAACTCC TCTGTGAGAC GTCACTGGGC TACAAGATGG ATGACCTCCT GACTTCCTAT
    ATTAGCCAGA TGCTTACGGC CATGAGCAAA CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92774
    Clone ID Related Accession (Same CDS sequence) XM_015474829.1 , XM_015466589.1
    Accession Version XM_015474829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330315.1
    CDS2164..8697
    Translation

    Target ORF information:

    RefSeq Version XM_015474829.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92774
    Clone ID Related Accession (Same CDS sequence) XM_015474829.1 , XM_015466589.1
    Accession Version XM_015466589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322075.1
    CDS2164..8697
    Translation

    Target ORF information:

    RefSeq Version XM_015466589.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGGCTAC AAGATGGATG ACCTCCTGAC TTCCTATATT
    AGCCAGATGC TTACGGCCAT GAGCAAACAG CGGGGCTCCA GGAGTGGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92775
    Clone ID Related Accession (Same CDS sequence) XM_015466590.1 , XM_015474830.1
    Accession Version XM_015466590.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGTCTCA TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322076.1
    CDS2164..8619
    Translation

    Target ORF information:

    RefSeq Version XM_015466590.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGTCTCA TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92775
    Clone ID Related Accession (Same CDS sequence) XM_015466590.1 , XM_015474830.1
    Accession Version XM_015474830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGTCTCA TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330316.1
    CDS2164..8619
    Translation

    Target ORF information:

    RefSeq Version XM_015474830.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG GAGCGAAGTT GGCGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAT
    GAGTACACCT TCACCTCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTCCTG
    GAGGGGCTCC GCAAGAGGTC TAAGTATGTG GTGGCCCTGC AGGACAACCC CAACCCTGCG
    GGCGAGGAGT CCGGCTTCCT CAGCTTCGCC AAGGGGGACC TCCTCATCCT GGACCACGAC
    ACGGGCGAAC AGGTGATGAA CTCGGGCTGG GCCAGCGGCA TCAACGAGAG GACCAAGCAG
    CGTGGGGACT TCCCCACTGA CTGCGTTTAT GTCATGCCTA CTGTCACTAT GCCACCGAGG
    GAGATTGTGG CCCTGGTCAC CATGACCCCC GACCAGAGGC AGGATGTTAT CCGACTCCTG
    CAGCTGCGAA CGGCAGAGCC CGAGGCGCGC ACCAAGCCCT ACACACTGGA GGAGTTTTCC
    TACGACTACT TCAGGCCCCC ACCCAAGCAC ACACTGAGCC GCGTCATGGT GTCCAAGGCC
    CGCGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAGCCGC TCAAGCAGGC CCTGCTCAAG
    AAGATCCTGG GCAGCGAGGA GCTCTCACAG GAGGCCTGCA TGGCCTTCAC TGCTGTGCTC
    AAGTACATGG GTGACTACCC GTCCAAGAGG ATGCGCTCCG TCAATGAGCT CACCGACCAG
    ATCTTCGAGG GAGCCCTGAA GGCCGAGCCC CTCAAGGACG AGGTGTATGT GCAGATCCTG
    AAGCAGCTGA CTGACAACCA CATCAGGTAC AGTGAGGAGC GGGGCTGGGA GCTACTCTGG
    TTGTGCACAG GGCTCTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG
    CAGTCCCGCA AACACTGCCC ACTTGCCATC GACTGCCTGC AGCGACTTCA GAAAGCCCTG
    AGAAATGGGT CCCGGAAGTA CCCCCCACAC TTGGTGGAGG TGGAAGCCAT CCAGCACAAA
    ACCACCCAGA TTTTCCACAA GGTCTACTTT CCTGATGACA CTGACGAGGC CTTTGAGGTG
    GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAATATCG CCGCCCGGCT GCTCCTCAAG
    TCCTCCGAGG GATTCAGCCT CTTTGTCAAA ATTGTAGATA AGGTCATCAG CGTCCCTGAG
    AATGACTTCT TCTTCGACTT TGTACGACAT CTGACGGACT GGATAAAGAA AGCCCGGCCT
    GTCAGGGATG GAGTCGTGCC CTCGCTGACC TACCAAGTGT TCTTCATGAA GAAGCTGTGG
    ACCACCACAG TGCCAGGGAA GGACCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG
    TTGCCCAAGT ACCTCCGAGG CTACCACAAG TGCACGCGGG AAGAGGTGCT GCAGCTGGGG
    GCGCTCATCT ATAGGGTCAA GTTCGAGGAG GATAAGTCCT ACTTCCCCAG TATCCCCAAG
    CTGCTCCGGG AGCTGGTGCC CCAGGACCTC ATCCGGCAGG TCTCGCCTGA CGACTGGAAG
    AGGTCCATCG TCGCCTATTT CAACAAGCAC GCGGGGAAGT CCAAGGAGGA GGCCAAGCTG
    GCTTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTCGGCT CAGCCTTCTT TGAGGTGAAG
    CAAACTACAG AGCCAAACTT TCCTGAGATC CTCTTAATTG CCATCAACAA GTATGGGGTC
    AGCCTCATTG ATCCGAGAAC CAAGGACATC CTGACCACTC ACCCCTTCAC CAAGATCTCC
    AACTGGAGCA GCGGCAACAC CTACTTCCAC ATCACCATTG GGAACCTGGT GCGTGGGAGC
    AAACTCCTCT GTGAGACGTC ACTGGTCTCA TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53031
    Clone ID Related Accession (Same CDS sequence) XM_010812768.2 , XM_865073.8
    Accession Version XM_010812768.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCAGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGATAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGAGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811070.1
    CDS2164..8790
    Translation

    Target ORF information:

    RefSeq Version XM_010812768.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812768.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCAGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGATAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGAGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53031
    Clone ID Related Accession (Same CDS sequence) XM_010812768.2 , XM_865073.8
    Accession Version XM_865073.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VIIa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_865073.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCAGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGATAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGAGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_870166.6
    CDS2164..8790
    Translation

    Target ORF information:

    RefSeq Version XM_865073.8
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VIIA (MYO7A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_865073.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGTGATTC TTCAGCAGGG CGACTACGTC TGGATGGACC TGAGATCCGG ACAGGAGTTT 
    GATGTGCCCA TTGGAGCGGT GGTGAAGCTC TGTGATTCTG GGCAGATCCA GGTGGTGGAC
    GATGAAGGCA ATGAACACTG GATTTCCCCG CAGAACGCCA CGCACATCAA GCCCATGCAC
    CCCACTTCAG TCCACGGCGT GGAGGACATG ATCCGCTTGG GGGACCTCAA TGAAGCCGGC
    ATCCTGCGCA ACCTGCTCAT TAGATACCGA GACCATCTCA TCTACACCTA CACGGGCTCC
    ATCTTGGTGG CTGTGAACCC CTACCAGCTG CTCTCTATCT ACTCGCCAGA ACACATCCGC
    CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAT
    TGCTACTTCA ATATGAAGCG CAACAGCCGT GACCAGTGCT GCATCATCAG TGGGGAGTCT
    GGGGCAGGGA AGACAGAGAG CACGAAGCTG ATCCTGCAGT TCCTGGCGGC CATCAGCGGG
    CAGCACTCGT GGATTGAACA GCAGGTGCTG GAAGCAACTC CCATCTTGGA AGCGTTTGGG
    AATGCTAAGA CCATCCGCAA TGACAACTCA AGCCGCTTCG GGAAGTACAT CGACATCCAC
    TTCAACAAGC GGGGCGCCAT CGAGGGCGCC AGGATCGAGC AGTACCTGCT GGAGAAGTCA
    CGAGTCTGTC GCCAGGCTCC AGATGAAAGG AATTACCACG TGTTCTACTG CATGCTGGAG
    GGCATGAATG AGGAGCAGAA GAAGAAGCTG GGCCTGGGCC AGGCCACGGA CTATAACTAC
    TTGGCCATGG GTAACTGCGT AATCTGTGAG GGCCGCGAGG ACAGCCAGGA ATATGCCAAC
    ATCCGCTCAG CCATGAAGGT GCTCATGTTC ACGGACACGG AGAACTGGGA GATCTCGAAG
    CTGCTAGCTG CCATCCTGCA TCTGGGCAAC CTGCAGTATG AGGACCGCAC GTTTGAAAAC
    CTGGATGCCT GCGAGGTCCT CTTCTCGCCA TCCCTGGCCA CAGCCGCGTC CTTGCTGGAG
    GTGAACCCCC GAGACCTGAT GAACTGCCTG ACCAGCCGCA CCCTCATCAC TCGAGGGGAG
    ACAGTGTCCA CTCCGCTCAG CAGGGAGCAG GCGCTGGACG TGCGAGATGC CTTTGTCAAG
    GGCATCTATG GGCGGCTCTT CGTGTGGATC GTGGACAAGA TCAATGCAGC CATTTATAAG
    CCCCCTTCCC AGGAAGTGAA GAACTCTCGC CGGTCCATTG GTCTCCTGGA CATTTTTGGG
    TTCGAGAACT TTGCAGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTTGC CAATGAGCAC
    CTGCAGCAGT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA TGACCTGGAG
    AGCATTGACT GGCTGCACAT TGAGTTCACA GACAACCAGG ATGCCCTGGA CATGATCGCC
    AACAAACCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG
    GACACCACCA TGTTGCATAA GCTCAACTCC CAGCACAAGC TCAACTCGAA CTACATTCCC
    CCCAAGAACA ATTACGAGAC CCAGTTTGGG ATCAACCACT TTGCAGGCAT CGTTTACTAT
    GAGAGTCAAG GCTTCCTGGA GAAGAACCGG GACACTCTGC ATGGAGACAT CATCCAGCTG
    GTGCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT TGCCATGGGC
    GCCGAGACCA GGAAGCGCTC GCCCACACTC AGCAGCCAGT TTAAGCGGTC GCTGGAGCTG
    CTGATGCGGA CACTGGGTGC CTGCCAGCCC TTCTTCGTGC GCTGCATCAA GCCCAATGAG
    TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGTGTGC GCCAGCTGCG GTACTCGGGC
    ATGATGGAGA CCATCCGCAT CCGCCGAGCC GGCTACCCCA TCCGCTACAG CTTCGTGGAG
    TTTGTGGAGC GGTACCGAGT GTTGCTGCCT GGCGTGAAGC CGGCCTACAA GCAGGACGAC
    CTTCGGGGGA CATGTCAGCG CATGGCTGAG GCTGTGCTGG GCACCCATGA CGACTGGCAG
    ATTGGCAAAA CCAAGATTTT TCTGAAGGAC CACCATGACA TGCTGCTGGA GGTGGAGCGG
    GACAAGGCCA TCACCGACAG AGTCATCCTC CTCCAGAAAG TCATCCGGGG CTTCAAAGAC
    AGGTCCAACT TCCTGAAGCT GAAGAATGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC
    CACAACTGCA GGAGGAACTA CGGGCTGATG CGGCTGGGCT TCCTGCGGCT GCAGGCCCTG
    CACCGCTCCC GGAAGCTGCA CCAGCAGTAC TGCCTGGCCC GCCGGCGCAT CATCGGGTTC
    CAGGCCCGCT GCCGGGCCTA CCTGGTACGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG
    ATCACCGTGC AGGCCTACGC CCGGGGCATG ATCGCCCGCA GGCTGCACCG GCGCCTCAGG
    GCCGAGTACC TGCGGCGCCT GGAGGCTGAG AAAATGCGGC TGGCAGAGGA GGAGAAGCTC
    CGGAAGGAGA TGAGTGCCAA GAAGGCCAAG GAGGAGGCCG AACGCAAACA TCAGGAGCGC
    CTGGCCCAGC TGGCCCGCGA GGATGCAGAG CGAGAGCTGA AGGAGAAGGA GGAGGCTCGG
    AGGAAGAAGG AACTCCTGGA GCAGATGGAG AGGGCCCGCC ATGAGCCCAT CAATCACTCG
    GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCGG GCGGCCTGCC AGGCCAGGAG
    GGCCAGGCAC CTAGTGGCTT CGAGGACCTG GAGCGCGGGC GGAGGGAGAT GGTAGAAGAG
    GACCTGGATG CAGCCCTGCC CCTGCCCGAC GAAGATGAGG AAGATCTCTC CGAGTATAAA
    TTCGCCAAGT TCGCTGCCAC TTACTTCCAG GGCACGACCA CGCACACCTA CACCCGGCGG
    CCACTCAAGC AGCCACTGCT GTACCACGAT GATGAGGGTG ATCAGCTGGC GGCCCTGGCA
    GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAGGTA CCACACGGCC
    ATGAGTGACG GCAGCGAGAA GATTCCCGTG ATGACCAAGA TTTACGAGAC CCTGGGCAAG
    AAGACCTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GTGAGGCCCA GCTCCCCGAT
    GGGCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCACT TGACCTTGAA AAAGAAGTCC
    AAACTGACGG AGGAGGTGAC CAAGAGGCTG CATGATGGGG AGTCCACGGT GCAGGGCAAC
    AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATTGGCAAC
    GGCATCCTGC GGCCAGCACT CCGGGATGAG ATCTACTGCC AGATCAGCAA GCAGCTCACT
    CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATCC TCGTGTCTCT CTGCGTGGGC
    TGCTTTGCCC CCTCTGAGAA GTTTGTTAAG TACCTGCGGA ACTTCATCCA TGGGGGACCC
    CCTGGCTACG CCCCGTACTG TGAGGAGCGA CTCAGGAGGA CCTTTGTCAA TGGGACACGG
    ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG
    CCTGTGACGT TCATGGACGG GACCACCAAG ACGCTGCTGA CGGACTCGGC GACCACGGCC
    AAGGAGCTCT GCAATGCGCT GGCCGACAAG ATCTCGCTCA AGGACCGCTT CGGGTTCTCC
    CTCTACATCG CCTTGTTTGA TAAGGTGTCC TCCCTGGGCA GTGGCAGCGA CCACGTCATG
    GACGCTATCT CCCAATGTGA ACAGTACGCC AAGGAGCAGG GTGCCCAGGA GCGCAACGCC
    CCGTGGAGGC TCTTCTTCCG CAAGGAGGTC TTCACGCCCT GGCACAACCC CTCAGAAGAC
    AATGTGGCCA CTAACCTCAT CTACCAGCAG GTGGTGCGAG GGGTCAAGTT TGGGGAGTAC
    AGGTGTGAGA AGGAGGATGA CCTGGCTGAA CTGGCCTCCC AGCAGTACTT CGTGGACTAT
    GGCTCCGAAA TGATCCTGGA GCGCCTGCTG AACCTCGTTC CCACCTACAT CCCTGACCGC
    GAGATCACGC CACTGAGGAC GCTGGAGAAG TGGGCCCAGC TGGCCATTGC GGCCCACAAG
    AAGGGAATTT ATGCCCAGAG GAGAACTGAT GCTCAGAAGG TCAAAGAGGA TGTGGTCAAC
    TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAGTTCTCA
    GGCCCCAACC TCCCCAAGAA CGACGTCATT GTGGCGGTCA ACTGGACAGG TGTCTACTTT
    GTGGATGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CCGAGATCAT GGCCGTGTCC
    AGCAGTAGGG AGTGCCGTAT CTTGCTCTCG CTGGGCTGCT CTGACCTGGG CTGTGCTGGA
    CTGACCCCGA CGGGGCCCTG TTCTCCGTGT TGGTCATGCA GGGGAGCGAA GTTGGCGGCC
    CCCAGCTTCA CGCTGGCCAC CATCAAGGGG GATGAGTACA CCTTCACCTC CAGCAATGCT
    GAGGACATCC GTGACCTGGT GGTCACCTTC CTGGAGGGGC TCCGCAAGAG GTCTAAGTAT
    GTGGTGGCCC TGCAGGACAA CCCCAACCCT GCGGGCGAGG AGTCCGGCTT CCTCAGCTTC
    GCCAAGGGGG ACCTCCTCAT CCTGGACCAC GACACGGGCG AACAGGTGAT GAACTCGGGC
    TGGGCCAGCG GCATCAACGA GAGGACCAAG CAGCGTGGGG ACTTCCCCAC TGACTGCGTT
    TATGTCATGC CTACTGTCAC TATGCCACCG AGGGAGATTG TGGCCCTGGT CACCATGACC
    CCCGACCAGA GGCAGGATGT TATCCGACTC CTGCAGCTGC GAACGGCAGA GCCCGAGGCG
    CGCACCAAGC CCTACACACT GGAGGAGTTT TCCTACGACT ACTTCAGGCC CCCACCCAAG
    CACACACTGA GCCGCGTCAT GGTGTCCAAG GCCCGCGGCA AGGACCGGCT GTGGAGCCAC
    ACGCGGGAGC CGCTCAAGCA GGCCCTGCTC AAGAAGATCC TGGGCAGCGA GGAGCTCTCA
    CAGGAGGCCT GCATGGCCTT CACTGCTGTG CTCAAGTACA TGGGTGACTA CCCGTCCAAG
    AGGATGCGCT CCGTCAATGA GCTCACCGAC CAGATCTTCG AGGGAGCCCT GAAGGCCGAG
    CCCCTCAAGG ACGAGGTGTA TGTGCAGATC CTGAAGCAGC TGACTGACAA CCACATCAGG
    TACAGTGAGG AGCGGGGCTG GGAGCTACTC TGGTTGTGCA CAGGGCTCTT CCCACCCAGC
    AACATCCTCC TGCCCCACGT GCAGCGCTTC CTGCAGTCCC GCAAACACTG CCCACTTGCC
    ATCGACTGCC TGCAGCGACT TCAGAAAGCC CTGAGAAATG GGTCCCGGAA GTACCCCCCA
    CACTTGGTGG AGGTGGAAGC CATCCAGCAC AAAACCACCC AGATTTTCCA CAAGGTCTAC
    TTTCCTGATG ACACTGACGA GGCCTTTGAG GTGGAGTCCA GCACCAAGGC CAAGGACTTC
    TGCCAGAATA TCGCCGCCCG GCTGCTCCTC AAGTCCTCCG AGGGATTCAG CCTCTTTGTC
    AAAATTGTAG ATAAGGTCAT CAGCGTCCCT GAGAATGACT TCTTCTTCGA CTTTGTACGA
    CATCTGACGG ACTGGATAAA GAAAGCCCGG CCTGTCAGGG ATGGAGTCGT GCCCTCGCTG
    ACCTACCAAG TGTTCTTCAT GAAGAAGCTG TGGACCACCA CAGTGCCAGG GAAGGACCCC
    ATGGCCGATT CCATCTTCCA CTATTACCAG GAGTTGCCCA AGTACCTCCG AGGCTACCAC
    AAGTGCACGC GGGAAGAGGT GCTGCAGCTG GGGGCGCTCA TCTATAGGGT CAAGTTCGAG
    GAGGATAAGT CCTACTTCCC CAGTATCCCC AAGCTGCTCC GGGAGCTGGT GCCCCAGGAC
    CTCATCCGGC AGGTCTCGCC TGACGACTGG AAGAGGTCCA TCGTCGCCTA TTTCAACAAG
    CACGCGGGGA AGTCCAAGGA GGAGGCCAAG CTGGCTTTCC TGAAGCTCAT CTTCAAGTGG
    CCCACCTTCG GCTCAGCCTT CTTTGAGGTG AAGCAAACTA CAGAGCCAAA CTTTCCTGAG
    ATCCTCTTAA TTGCCATCAA CAAGTATGGG GTCAGCCTCA TTGATCCGAG AACCAAGGAC
    ATCCTGACCA CTCACCCCTT CACCAAGATC TCCAACTGGA GCAGCGGCAA CACCTACTTC
    CACATCACCA TTGGGAACCT GGTGCGTGGG AGCAAACTCC TCTGTGAGAC GTCACTGGGC
    TACAAGATGG ATGACCTCCT GACTTCCTAT ATTAGCCAGA TGCTTACGGC CATGAGCAAA
    CAGCGGGGCT CCAGGAGTGG CAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.