The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa221699 | |
| Clone ID Related Accession (Same CDS sequence) | XM_025000716.1 | |
| Accession Version | XM_025000716.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA topoisomerase 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGCTGA GCCCCAAAGC GGAAGAAGTA GCTACGTTCT TTGCAAAAAT GCTCGACCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024856484.1 |
| CDS | 164..1723 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_025000716.1 |
1 | ATGAAGCTGA GCCCCAAAGC GGAAGAAGTA GCTACGTTCT TTGCAAAAAT GCTCGACCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa37757 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001206487.1 , NM_001206487.2 | |
| Accession Version | NM_001206487.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-27 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA topoisomerase 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE228015.1, BC118461.1, DAAA02036644.1, DV927671.1, EE351723.1, EH133197.1 and EE369776.1. On or before May 19, 2011 this sequence version replaced XM_002692463.1, XM_614691.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403639, SAMN02403640 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGTGGGG ACCATCTCCA CAACGATTCC CAGATCGAGG CGGATTTCCG ACTGAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001193416.1 |
| CDS | 232..2541 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001206487.1 |
1 | ATGAGTGGGG ACCATCTCCA CAACGATTCC CAGATCGAGG CGGATTTCCG ACTGAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa37757 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001206487.1 , NM_001206487.2 | |
| Accession Version | NM_001206487.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-15 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA topoisomerase 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE228015.1, BC118461.1, NKLS02000013.1, DV927671.1, EE351723.1, EH133197.1 and EE369776.1. On Jun 16, 2018 this sequence version replaced NM_001206487.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.840.1, SRR5571255.24168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGTGGGG ACCATCTCCA CAACGATTCC CAGATCGAGG CGGATTTCCG ACTGAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001193416.1 |
| CDS | 232..2541 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001206487.2 |
1 | ATGAGTGGGG ACCATCTCCA CAACGATTCC CAGATCGAGG CGGATTTCCG ACTGAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo. |
Cytogenetic alignment of the bovine chromosome 13 genome map by fluorescence in-situ hybridization of human chromosome 10 and 20 comparative markers. |
