GPATCH8 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010816406.2	XP_010814708.1	G patch domain-containing protein 8 isoform X3
XM_010816408.2	XP_010814710.1	G patch domain-containing protein 8 isoform X3
XM_003583622.4	XP_003583670.2	G patch domain-containing protein 8 isoform X4
XM_024980919.1	XP_024836687.1	G patch domain-containing protein 8 isoform X2
XM_010816409.2	XP_010814711.1	G patch domain-containing protein 8 isoform X3
XM_005220960.3	XP_005221017.1	G patch domain-containing protein 8 isoform X2
XM_010824215.2	XP_010822517.2	G patch domain-containing protein 8 isoform X3
XM_003587454.5	XP_003587502.1	G patch domain-containing protein 8 isoform X2
XM_024980917.1	XP_024836685.1	G patch domain-containing protein 8 isoform X2
XM_010824219.2	XP_010822521.2	G patch domain-containing protein 8 isoform X3
XM_024980916.1	XP_024836684.1	G patch domain-containing protein 8 isoform X2
XM_024980918.1	XP_024836686.1	G patch domain-containing protein 8 isoform X2
XM_010824217.2	XP_010822519.2	G patch domain-containing protein 8 isoform X3
XM_015468184.1	XP_015323670.1	G patch domain-containing protein 8 isoform X1
XM_010824216.2	XP_010822518.2	G patch domain-containing protein 8 isoform X3
XM_015458900.1	XP_015314386.1	G patch domain-containing protein 8 isoform X1
XM_010816407.2	XP_010814709.1	G patch domain-containing protein 8 isoform X3
XM_015458901.1	XP_015314387.1	G patch domain-containing protein 8 isoform X3
XM_015468185.1	XP_015323671.1	G patch domain-containing protein 8 isoform X3
XM_005195765.3	XP_005195822.2	G patch domain-containing protein 8 isoform X2
XM_003587454.4	XP_003587502.1	G patch domain-containing protein 8 isoform X4
XM_005220960.4	XP_005221017.1	G patch domain-containing protein 8 isoform X1
XM_024980921.1	XP_024836689.1	G patch domain-containing protein 8 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa30196	XM_024980917.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_024980918.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_024980916.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_003583622.4 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_003587454.5 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_024980921.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30196	XM_024980919.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30195	XM_005195765.3 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa30195	XM_005220960.4 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa93991	XM_015458900.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa93991	XM_015468184.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa52927	XM_010816408.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_015458901.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010824219.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010816409.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_015468185.1 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010816406.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010824216.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010824215.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010824217.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52927	XM_010816407.2 Latest version!	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa30196	XM_003587454.4	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa30195	XM_005220960.3	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
Hide Old Accession Versions >>

GPATCH8 ( XM_003583622.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583670.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_003583622.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583670.2
CDS	272..4567
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_003583622.4
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583622.4

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_024980919.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836687.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_024980919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836687.1
CDS	294..4589
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_024980919.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980919.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_003587454.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587502.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_003587454.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587454.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587502.1
CDS	288..4583
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_003587454.5
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587454.5

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_024980917.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836685.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

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CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_024980917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836685.1
CDS	299..4594
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_024980917.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980917.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_024980916.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836684.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_024980916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836684.1
CDS	300..4595
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_024980916.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980916.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_024980918.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836686.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_024980918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836686.1
CDS	308..4603
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_024980918.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980918.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_003587454.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587502.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.

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CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_003587454.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587502.1
CDS	272..4567
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_003587454.4
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587454.4

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_024980921.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836689.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

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CloneID	OBa30196
Clone ID Related Accession (Same CDS sequence)	XM_003583622.4 , XM_024980919.1 , XM_003587454.5 , XM_024980917.1 , XM_024980916.1 , XM_024980918.1 , XM_003587454.4 , XM_024980921.1
Accession Version	XM_024980921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836689.1
CDS	221..4516
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_024980921.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980921.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGAG ATTGAAAGAT CTCAAGCAGA GAGAGTTTGC TCGAAATGTC 
TCCTCAAGAT CCCGCAAGGA TGAGAAGAAA CAGGAGAAAG CCCTACGGCG GCTCCACGAG 
TTGGCAGAGC AAAGAAAACA AGCTGAATGT GCACCTGGAA GTGGTCCCAT GTTCAGACCA 
ACCACAGTGG CTGTAGATGA AGAAGCTGGA GATGATGAGA AGGATGAATC AGCAACAAAC 
AGTGGCACAA GTACCGCTGC CGCTTGTGGC CTGGGATCTG AATTCTCCAC GGATAAAGGA 
GGCCCATTCA CTGCAGTACA AATCACTAAT ACCACTGGGC TGGCACAGGC TCCTGGGCCA 
GCCTCTCAAG GCATCAGCTT TGGCATTAAG AATAATCTGG GGACCCCACT ACAAAAATTG 
GGAGTGTCAT TTTCCTTTGC CAAGAAGGCT CCTGTCAAAC TCGAATCAAT AGCATCAGTT 
TTCAAGGACC ACGCAGAGGA AGGGACCTCT GATGATGGAA CAAAAGCTGA TGAAAAGGGT 
GCAGACCAAG GACTGCAGAA GGTGGGAGAT TCTGATGGTA GCAGTAATCT CGATGGTAAA 
AAAGAGGATG AAGACCCTCA GGACGGAGGG TCCCTTGCCT CAACATTATC TAAGTTAAAA 
AGAATGAAGC GAGAAGAAGG AGCTGGGGCT ACAGAGCCAG AGTATTACCA CTACATCCCC 
CCAGCACACT GCAAAGTAAA ACCTAATTTT CCTTTTCTGC TCTTTATGAG AGCCAGTGAA 
CAAATGGAAG GTGATAATAG TACACACCCA AAGAATGCCC TAGACAGCAA AAAAAGTAGT 
TCTCCCAAGC CTAAAGGCTG CATCAAGGTG ACAGCAAGCC AAGGACCGGA AAAGACAGTC 
AGTGAAGTCT CCGAACAGCA AATGGAAACC AGTGCGGCTG AGCCCTCAGA GCCTGGAAGC 
AAAGCTGAGA CAAAGAAGCC CTCAGGAGGG GATGTAAGTG AGCATAGTTT AGAGATTCAG 
AGTCAGAAGG ATTCAGAGAT CCAAATGTGT GAGTCTGACC CTCATAAAGA AACTTCTCAG 
GCTACTCCAG CAGGGAGAGA AAGCCGGGAG GGACCCAAAC ATCCAACTGG TCCTTTCTTT 
CCGGTTTTAA GCAAAGATGA AAGCACTGCC CTCCAGTGGC CATCAGAACT GTTAATTTTC 
ACGAAGGCAG AACCCTCCAT TTCGTACAGT TGTAACCCTT TGTATTTTGA CTTCAAGCTT 
TCAAGGAACA AAGATGCCAG AGCAAAAGGG ACAGAAAAGC CAAAGGACAT AGGAGGCTCC 
TCAAAGGACC ATCTCCAAGG CCTTGATCCT AGTGAGCTAC ATAAAAGCCA GGAGGAGGAA 
GAGAGTGTTC AACATTCTTC AGGAGGCAGA ATCGATGCAC CTGCTTCAGG GGCTGCCTGT 
AGCGGCCTAA ATAAGCAGGA GCCTGGGGGT AGCCATGGAT CAGAGACAGA AGACACAGGG 
AGAAGCCTTC CTAGCAAGAA AGAACGATCT GGGAAGTCCC ACCGACACAA AAAGAAAAAG 
AAGCACAAAA AATCCAGCAA ACATAAACGG AAACACAGGG CTGACCCAGA AGAGAAAAGC 
TCTAAGGCAG AATCTGGGGA GAAGTCTAAG AAGCGCAAGA AGCGAAAACG AAAAAAGAAT 
AAATCATCAG CCCCAGCAGA TTCTGAACGG GGGCCCAAAC CAGAACCCCC TGGTAGTGGT 
AGCCCTGCAC CACCAAGAAG ACGGCGGCGA GCCCAGGATG ATTCGCAGCG GAGATCCCTT 
CCAGCTGAGG AAGGGAGCAG CGGCAAGAAG GATGAAGGCG GAGGCAGTGG GAGCGCCCAA 
GATCATAGTG GGAGGAAACA CAGGGGTGAA CCTCCCGCAT CATCCTGCCA GCGAAGAGCT 
GGCACCAAGC GGAGCAGCCG ATCCAGCCAT CGAAGTCGAC CCAGTAGTGG AGATGAGGAC 
AGTGATGATG CATCATCACG CCGGCTGCAC CAGAAGTCTC CATCCCAGTA CAGTGAGGAG 
GAGGAGGAGG AGGAGGAGGA AGAGGAAGAG TCGGGCAGTG AGCATTCCCG AAGCCGCTCA 
CGGTCTGGCC GGCACCATTC CTCTCGCCAT TCCTCCCGGC GCTCTTACTC CAGTAGCTCA 
GATGCTTCTT CAGACCAGAG CTGCTATAGT AGACAACACA GTTACTCTGA TGACAGCTAT 
AGTGACTATA GTGACCGGTC ACGAAGGCAC TCCAAGCGCT CCCATGACTC TGATGACTCA 
GACTACGCCA GCTCCAAGCA CCGGTCCAAG CGGCACAAAT ATTCATCTTC TGATGATGAC 
TATAGCCTCA GTGGCAGCCA GTCCCGAAGC CGATCTCGGA GTCATACCAG AGAGCGCTCA 
AGATCCAGGG GCCGTAGCCG CAGCAGCAGC TGTAGTCGCA GCCGGAGCAA AAGGAGAAGC 
CGCAGCACCA CAGCCCACAG CTGGCAGCGG AGTCGGAGCT ATAGCCGGGA CCGCAGCCGC 
AGCACCCGGA GCCCTTCACA GAGATCAGGC TCCAGGAAGG GATCGTGGGG GCACGAGAGC 
CCTGAAGAGA GGCGTTCTGG TCGTCGAGAC TTCATTCGCT CTAAAATCTA CCGCTCCCAG 
TCTCCCCATT ACTTCCGATC AGGCCGGGGA GAAGGTCCTG GGGAGAAGAA GAAAGAAGAC 
GGCAGAGGAG ATGATGGTAA GGGAATAGGC CCACCCTCCC AGAACAGCAG CGTTGGCCCA 
GGAAGAGGGT CAGAAGGTGA CTGCAGCCCT GAAGACAAGA ACTCTGTCAC TGCCAAGCTG 
CTGCTGGAAA AGATTCAGTC AAGGAAAGTG GAGAGGAAAC CCAGTATGAG TGAGGAGGTG 
CTGGCCACCC CTAATAAAGC CGGTCTCAAG CTCAAGGACC CCCCACAAGG TTATTTTGGA 
CCCAAGCTCC CCCCTTCTCT TGGCAACAAG CCTGTCCTTC CTCTGATAGG GAAGCTCCCA 
GCTACCCGAA AGCCCAACGC CAAGAAATGT GAAGAGTCTG GCTTAGAAAG GGGAGAAGAG 
CAAGAACAGT CAGAGACAGA AGAAGGGCCT CCAGGGAATA GTGATGCTCC ATTTGGACAT 
CAGTTCCCCT CAGAGGAGAC AGCTGGCCCC TTATCAGACC CACCCCCAGA AGAGCCAAAG 
TCTGAAGAAG CTACTGCTGA TCACCCTGTG GCTCCACTAG GCACCCCAGT GCACTCTGAC 
TGCTATCCTG GGGACCCATC CATCTCCCAT AACTACCTCC CTGACCCCAG CGACGGGGAC 
ACCCTTGAGT CCCTGGATAG TGGCAGTCAG CCAGGCCCCG TGGAGTCCAG CTTGCTGCCT 
ATAGCATCAG ACCTCGAGCC CTTCCCCGGT TACGCACCGC CCAGTGGGGA ACCCAGTATT 
GAGTCAGTTG ATGGAGCCGA GGATGCATCC CTCGCCCCAC TGGAGAGCCA GCCCATCACC 
TTCACTCCCG AGGAGATGGA GAAGTACAGC AAGCTCCAGC AGGCCGCACA GCAGCACATC 
CAGCAGCAGC TTCTGGCCAA GCAGGTGAAG GCCTTCCCCG CTTCGGCCGC CCTGGCCCCA 
GCCACTCCAG CCTTGCAGCC TATCCACATT CAGCAGCCGG CCACGGCCTC TGCCACCTCC 
ATCACAACTG TGCAGCATGC CATCCTACAG CATCATGCCG CAGCAGCTGC CGCTGCCATT 
GGCATTCACC CCCACCCTCA CCCCCAGCCA CTAGCCCAAG TGCATCATAT CCCCCAGCCC 
CACCTGACCC CCATCTCCTT GTCCCACCTC ACTCACTCCA TCATCCCTGG TCATCCCGCC 
ACCTTTCTGG CTAGCCACCC CATCCACATC ATTCCTGCCT CAGCCATCCA TCCCGGGCCC 
TTCACCTTCC ACCCTGTTCC ACACGCTGCC CTCTACCCTA CCCTGCTGGC TCCCCGGCCT 
GCTGCAGCAG CTGCCACTGC CCTCCACCTT CACCCACTAC TTCACCCCAT CTTCTCAGGT 
CAGGACCTGC AGCACCCCCC CAGCCATGGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_005220960.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221017.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30195
Clone ID Related Accession (Same CDS sequence)	XM_005220960.3 , XM_005195765.3 , XM_005220960.4
Accession Version	XM_005220960.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221017.1
CDS	175..4704
Translation	MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_005220960.3
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220960.3

ORF Insert Sequence:

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_005195765.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195822.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30195
Clone ID Related Accession (Same CDS sequence)	XM_005220960.3 , XM_005195765.3 , XM_005220960.4
Accession Version	XM_005195765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195822.2
CDS	175..4704
Translation	MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_005195765.3
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195765.3

ORF Insert Sequence:

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_005220960.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221017.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

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CloneID	OBa30195
Clone ID Related Accession (Same CDS sequence)	XM_005220960.3 , XM_005195765.3 , XM_005220960.4
Accession Version	XM_005220960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221017.1
CDS	199..4728
Translation	MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_005220960.4
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220960.4

ORF Insert Sequence:

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGAGATTGAA AGATCTCAAG CAGAGAGAGT TTGCTCGAAA TGTCTCCTCA 
AGATCCCGCA AGGATGAGAA GAAACAGGAG AAAGCCCTAC GGCGGCTCCA CGAGTTGGCA 
GAGCAAAGAA AACAAGCTGA ATGTGCACCT GGAAGTGGTC CCATGTTCAG ACCAACCACA 
GTGGCTGTAG ATGAAGAAGC TGGAGATGAT GAGAAGGATG AATCAGCAAC AAACAGTGGC 
ACAAGTACCG CTGCCGCTTG TGGCCTGGGA TCTGAATTCT CCACGGATAA AGGAGGCCCA 
TTCACTGCAG TACAAATCAC TAATACCACT GGGCTGGCAC AGGCTCCTGG GCCAGCCTCT 
CAAGGCATCA GCTTTGGCAT TAAGAATAAT CTGGGGACCC CACTACAAAA ATTGGGAGTG 
TCATTTTCCT TTGCCAAGAA GGCTCCTGTC AAACTCGAAT CAATAGCATC AGTTTTCAAG 
GACCACGCAG AGGAAGGGAC CTCTGATGAT GGAACAAAAG CTGATGAAAA GGGTGCAGAC 
CAAGGACTGC AGAAGGTGGG AGATTCTGAT GGTAGCAGTA ATCTCGATGG TAAAAAAGAG 
GATGAAGACC CTCAGGACGG AGGGTCCCTT GCCTCAACAT TATCTAAGTT AAAAAGAATG 
AAGCGAGAAG AAGGAGCTGG GGCTACAGAG CCAGAGTATT ACCACTACAT CCCCCCAGCA 
CACTGCAAAG TAAAACCTAA TTTTCCTTTT CTGCTCTTTA TGAGAGCCAG TGAACAAATG 
GAAGGTGATA ATAGTACACA CCCAAAGAAT GCCCTAGACA GCAAAAAAAG TAGTTCTCCC 
AAGCCTAAAG GCTGCATCAA GGTGACAGCA AGCCAAGGAC CGGAAAAGAC AGTCAGTGAA 
GTCTCCGAAC AGCAAATGGA AACCAGTGCG GCTGAGCCCT CAGAGCCTGG AAGCAAAGCT 
GAGACAAAGA AGCCCTCAGG AGGGGATGTA AGTGAGCATA GTTTAGAGAT TCAGAGTCAG 
AAGGATTCAG AGATCCAAAT GTGTGAGTCT GACCCTCATA AAGAAACTTC TCAGGCTACT 
CCAGCAGGGA GAGAAAGCCG GGAGGGACCC AAACATCCAA CTGGTCCTTT CTTTCCGGTT 
TTAAGCAAAG ATGAAAGCAC TGCCCTCCAG TGGCCATCAG AACTGTTAAT TTTCACGAAG 
GCAGAACCCT CCATTTCGTA CAGTTGTAAC CCTTTGTATT TTGACTTCAA GCTTTCAAGG 
AACAAAGATG CCAGAGCAAA AGGGACAGAA AAGCCAAAGG ACATAGGAGG CTCCTCAAAG 
GACCATCTCC AAGGCCTTGA TCCTAGTGAG CTACATAAAA GCCAGGAGGA GGAAGAGAGT 
GTTCAACATT CTTCAGGAGG CAGAATCGAT GCACCTGCTT CAGGGGCTGC CTGTAGCGGC 
CTAAATAAGC AGGAGCCTGG GGGTAGCCAT GGATCAGAGA CAGAAGACAC AGGGAGAAGC 
CTTCCTAGCA AGAAAGAACG ATCTGGGAAG TCCCACCGAC ACAAAAAGAA AAAGAAGCAC 
AAAAAATCCA GCAAACATAA ACGGAAACAC AGGGCTGACC CAGAAGAGAA AAGCTCTAAG 
GCAGAATCTG GGGAGAAGTC TAAGAAGCGC AAGAAGCGAA AACGAAAAAA GAATAAATCA 
TCAGCCCCAG CAGATTCTGA ACGGGGGCCC AAACCAGAAC CCCCTGGTAG TGGTAGCCCT 
GCACCACCAA GAAGACGGCG GCGAGCCCAG GATGATTCGC AGCGGAGATC CCTTCCAGCT 
GAGGAAGGGA GCAGCGGCAA GAAGGATGAA GGCGGAGGCA GTGGGAGCGC CCAAGATCAT 
AGTGGGAGGA AACACAGGGG TGAACCTCCC GCATCATCCT GCCAGCGAAG AGCTGGCACC 
AAGCGGAGCA GCCGATCCAG CCATCGAAGT CGACCCAGTA GTGGAGATGA GGACAGTGAT 
GATGCATCAT CACGCCGGCT GCACCAGAAG TCTCCATCCC AGTACAGTGA GGAGGAGGAG 
GAGGAGGAGG AGGAAGAGGA AGAGTCGGGC AGTGAGCATT CCCGAAGCCG CTCACGGTCT 
GGCCGGCACC ATTCCTCTCG CCATTCCTCC CGGCGCTCTT ACTCCAGTAG CTCAGATGCT 
TCTTCAGACC AGAGCTGCTA TAGTAGACAA CACAGTTACT CTGATGACAG CTATAGTGAC 
TATAGTGACC GGTCACGAAG GCACTCCAAG CGCTCCCATG ACTCTGATGA CTCAGACTAC 
GCCAGCTCCA AGCACCGGTC CAAGCGGCAC AAATATTCAT CTTCTGATGA TGACTATAGC 
CTCAGTGGCA GCCAGTCCCG AAGCCGATCT CGGAGTCATA CCAGAGAGCG CTCAAGATCC 
AGGGGCCGTA GCCGCAGCAG CAGCTGTAGT CGCAGCCGGA GCAAAAGGAG AAGCCGCAGC 
ACCACAGCCC ACAGCTGGCA GCGGAGTCGG AGCTATAGCC GGGACCGCAG CCGCAGCACC 
CGGAGCCCTT CACAGAGATC AGGCTCCAGG AAGGGATCGT GGGGGCACGA GAGCCCTGAA 
GAGAGGCGTT CTGGTCGTCG AGACTTCATT CGCTCTAAAA TCTACCGCTC CCAGTCTCCC 
CATTACTTCC GATCAGGCCG GGGAGAAGGT CCTGGGGAGA AGAAGAAAGA AGACGGCAGA 
GGAGATGATG GTAAGGGAAT AGGCCCACCC TCCCAGAACA GCAGCGTTGG CCCAGGAAGA 
GGGTCAGAAG GTGACTGCAG CCCTGAAGAC AAGAACTCTG TCACTGCCAA GCTGCTGCTG 
GAAAAGATTC AGTCAAGGAA AGTGGAGAGG AAACCCAGTA TGAGTGAGGA GGTGCTGGCC 
ACCCCTAATA AAGCCGGTCT CAAGCTCAAG GACCCCCCAC AAGGTTATTT TGGACCCAAG 
CTCCCCCCTT CTCTTGGCAA CAAGCCTGTC CTTCCTCTGA TAGGGAAGCT CCCAGCTACC 
CGAAAGCCCA ACGCCAAGAA ATGTGAAGAG TCTGGCTTAG AAAGGGGAGA AGAGCAAGAA 
CAGTCAGAGA CAGAAGAAGG GCCTCCAGGG AATAGTGATG CTCCATTTGG ACATCAGTTC 
CCCTCAGAGG AGACAGCTGG CCCCTTATCA GACCCACCCC CAGAAGAGCC AAAGTCTGAA 
GAAGCTACTG CTGATCACCC TGTGGCTCCA CTAGGCACCC CAGTGCACTC TGACTGCTAT 
CCTGGGGACC CATCCATCTC CCATAACTAC CTCCCTGACC CCAGCGACGG GGACACCCTT 
GAGTCCCTGG ATAGTGGCAG TCAGCCAGGC CCCGTGGAGT CCAGCTTGCT GCCTATAGCA 
TCAGACCTCG AGCCCTTCCC CGGTTACGCA CCGCCCAGTG GGGAACCCAG TATTGAGTCA 
GTTGATGGAG CCGAGGATGC ATCCCTCGCC CCACTGGAGA GCCAGCCCAT CACCTTCACT 
CCCGAGGAGA TGGAGAAGTA CAGCAAGCTC CAGCAGGCCG CACAGCAGCA CATCCAGCAG 
CAGCTTCTGG CCAAGCAGGT GAAGGCCTTC CCCGCTTCGG CCGCCCTGGC CCCAGCCACT 
CCAGCCTTGC AGCCTATCCA CATTCAGCAG CCGGCCACGG CCTCTGCCAC CTCCATCACA 
ACTGTGCAGC ATGCCATCCT ACAGCATCAT GCCGCAGCAG CTGCCGCTGC CATTGGCATT 
CACCCCCACC CTCACCCCCA GCCACTAGCC CAAGTGCATC ATATCCCCCA GCCCCACCTG 
ACCCCCATCT CCTTGTCCCA CCTCACTCAC TCCATCATCC CTGGTCATCC CGCCACCTTT 
CTGGCTAGCC ACCCCATCCA CATCATTCCT GCCTCAGCCA TCCATCCCGG GCCCTTCACC 
TTCCACCCTG TTCCACACGC TGCCCTCTAC CCTACCCTGC TGGCTCCCCG GCCTGCTGCA 
GCAGCTGCCA CTGCCCTCCA CCTTCACCCA CTACTTCACC CCATCTTCTC AGGTCAGGAC 
CTGCAGCACC CCCCCAGCCA TGGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_015468184.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323670.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

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CloneID	OBa93991
Clone ID Related Accession (Same CDS sequence)	XM_015468184.1 , XM_015458900.1
Accession Version	XM_015468184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGGCAGGCAC ACAGGATTAT TATGAATCTG AGATAATAGC TGATGTATTG 
AAAGCAAATC CTTCTAATTT GGAAGCCCAG ATCACAAGAA GATTGAAAGA TCTCAAGCAG 
AGAGAGTTTG CTCGAAATGT CTCCTCAAGA TCCCGCAAGG ATGAGAAGAA ACAGGAGAAA 
GCCCTACGGC GGCTCCACGA GTTGGCAGAG CAAAGAAAAC AAGCTGAATG TGCACCTGGA 
AGTGGTCCCA TGTTCAGACC AACCACAGTG GCTGTAGATG AAGAAGCTGG AGATGATGAG 
AAGGATGAAT CAGCAACAAA CAGTGGCACA AGTACCGCTG CCGCTTGTGG CCTGGGATCT 
GAATTCTCCA CGGATAAAGG AGGCCCATTC ACTGCAGTAC AAATCACTAA TACCACTGGG 
CTGGCACAGG CTCCTGGGCC AGCCTCTCAA GGCATCAGCT TTGGCATTAA GAATAATCTG 
GGGACCCCAC TACAAAAATT GGGAGTGTCA TTTTCCTTTG CCAAGAAGGC TCCTGTCAAA 
CTCGAATCAA TAGCATCAGT TTTCAAGGAC CACGCAGAGG AAGGGACCTC TGATGATGGA 
ACAAAAGCTG ATGAAAAGGG TGCAGACCAA GGACTGCAGA AGGTGGGAGA TTCTGATGGT 
AGCAGTAATC TCGATGGTAA AAAAGAGGAT GAAGACCCTC AGGACGGAGG GTCCCTTGCC 
TCAACATTAT CTAAGTTAAA AAGAATGAAG CGAGAAGAAG GAGCTGGGGC TACAGAGCCA 
GAGTATTACC ACTACATCCC CCCAGCACAC TGCAAAGTAA AACCTAATTT TCCTTTTCTG 
CTCTTTATGA GAGCCAGTGA ACAAATGGAA GGTGATAATA GTACACACCC AAAGAATGCC 
CTAGACAGCA AAAAAAGTAG TTCTCCCAAG CCTAAAGGCT GCATCAAGGT GACAGCAAGC 
CAAGGACCGG AAAAGACAGT CAGTGAAGTC TCCGAACAGC AAATGGAAAC CAGTGCGGCT 
GAGCCCTCAG AGCCTGGAAG CAAAGCTGAG ACAAAGAAGC CCTCAGGAGG GGATGTAAGT 
GAGCATAGTT TAGAGATTCA GAGTCAGAAG GATTCAGAGA TCCAAATGTG TGAGTCTGAC 
CCTCATAAAG AAACTTCTCA GGCTACTCCA GCAGGGAGAG AAAGCCGGGA GGGACCCAAA 
CATCCAACTG GTCCTTTCTT TCCGGTTTTA AGCAAAGATG AAAGCACTGC CCTCCAGTGG 
CCATCAGAAC TGTTAATTTT CACGAAGGCA GAACCCTCCA TTTCGTACAG TTGTAACCCT 
TTGTATTTTG ACTTCAAGCT TTCAAGGAAC AAAGATGCCA GAGCAAAAGG GACAGAAAAG 
CCAAAGGACA TAGGAGGCTC CTCAAAGGAC CATCTCCAAG GCCTTGATCC TAGTGAGCTA 
CATAAAAGCC AGGAGGAGGA AGAGAGTGTT CAACATTCTT CAGGAGGCAG AATCGATGCA 
CCTGCTTCAG GGGCTGCCTG TAGCGGCCTA AATAAGCAGG AGCCTGGGGG TAGCCATGGA 
TCAGAGACAG AAGACACAGG GAGAAGCCTT CCTAGCAAGA AAGAACGATC TGGGAAGTCC 
CACCGACACA AAAAGAAAAA GAAGCACAAA AAATCCAGCA AACATAAACG GAAACACAGG 
GCTGACCCAG AAGAGAAAAG CTCTAAGGCA GAATCTGGGG AGAAGTCTAA GAAGCGCAAG 
AAGCGAAAAC GAAAAAAGAA TAAATCATCA GCCCCAGCAG ATTCTGAACG GGGGCCCAAA 
CCAGAACCCC CTGGTAGTGG TAGCCCTGCA CCACCAAGAA GACGGCGGCG AGCCCAGGAT 
GATTCGCAGC GGAGATCCCT TCCAGCTGAG GAAGGGAGCA GCGGCAAGAA GGATGAAGGC 
GGAGGCAGTG GGAGCGCCCA AGATCATAGT GGGAGGAAAC ACAGGGGTGA ACCTCCCGCA 
TCATCCTGCC AGCGAAGAGC TGGCACCAAG CGGAGCAGCC GATCCAGCCA TCGAAGTCGA 
CCCAGTAGTG GAGATGAGGA CAGTGATGAT GCATCATCAC GCCGGCTGCA CCAGAAGTCT 
CCATCCCAGT ACAGTGAGGA GGAGGAGGAG GAGGAGGAGG AAGAGGAAGA GTCGGGCAGT 
GAGCATTCCC GAAGCCGCTC ACGGTCTGGC CGGCACCATT CCTCTCGCCA TTCCTCCCGG 
CGCTCTTACT CCAGTAGCTC AGATGCTTCT TCAGACCAGA GCTGCTATAG TAGACAACAC 
AGTTACTCTG ATGACAGCTA TAGTGACTAT AGTGACCGGT CACGAAGGCA CTCCAAGCGC 
TCCCATGACT CTGATGACTC AGACTACGCC AGCTCCAAGC ACCGGTCCAA GCGGCACAAA 
TATTCATCTT CTGATGATGA CTATAGCCTC AGTGGCAGCC AGTCCCGAAG CCGATCTCGG 
AGTCATACCA GAGAGCGCTC AAGATCCAGG GGCCGTAGCC GCAGCAGCAG CTGTAGTCGC 
AGCCGGAGCA AAAGGAGAAG CCGCAGCACC ACAGCCCACA GCTGGCAGCG GAGTCGGAGC 
TATAGCCGGG ACCGCAGCCG CAGCACCCGG AGCCCTTCAC AGAGATCAGG CTCCAGGAAG 
GGATCGTGGG GGCACGAGAG CCCTGAAGAG AGGCGTTCTG GTCGTCGAGA CTTCATTCGC 
TCTAAAATCT ACCGCTCCCA GTCTCCCCAT TACTTCCGAT CAGGCCGGGG AGAAGGTCCT 
GGGGAGAAGA AGAAAGAAGA CGGCAGAGGA GATGATGGTA AGGGAATAGG CCCACCCTCC 
CAGAACAGCA GCGTTGGCCC AGGAAGAGGG TCAGAAGGTG ACTGCAGCCC TGAAGACAAG 
AACTCTGTCA CTGCCAAGCT GCTGCTGGAA AAGATTCAGT CAAGGAAAGT GGAGAGGAAA 
CCCAGTATGA GTGAGGAGGT GCTGGCCACC CCTAATAAAG CCGGTCTCAA GCTCAAGGAC 
CCCCCACAAG GTTATTTTGG ACCCAAGCTC CCCCCTTCTC TTGGCAACAA GCCTGTCCTT 
CCTCTGATAG GGAAGCTCCC AGCTACCCGA AAGCCCAACG CCAAGAAATG TGAAGAGTCT 
GGCTTAGAAA GGGGAGAAGA GCAAGAACAG TCAGAGACAG AAGAAGGGCC TCCAGGGAAT 
AGTGATGCTC CATTTGGACA TCAGTTCCCC TCAGAGGAGA CAGCTGGCCC CTTATCAGAC 
CCACCCCCAG AAGAGCCAAA GTCTGAAGAA GCTACTGCTG ATCACCCTGT GGCTCCACTA 
GGCACCCCAG TGCACTCTGA CTGCTATCCT GGGGACCCAT CCATCTCCCA TAACTACCTC 
CCTGACCCCA GCGACGGGGA CACCCTTGAG TCCCTGGATA GTGGCAGTCA GCCAGGCCCC 
GTGGAGTCCA GCTTGCTGCC TATAGCATCA GACCTCGAGC CCTTCCCCGG TTACGCACCG 
CCCAGTGGGG AACCCAGTAT TGAGTCAGTT GATGGAGCCG AGGATGCATC CCTCGCCCCA 
CTGGAGAGCC AGCCCATCAC CTTCACTCCC GAGGAGATGG AGAAGTACAG CAAGCTCCAG 
CAGGCCGCAC AGCAGCACAT CCAGCAGCAG CTTCTGGCCA AGCAGGTGAA GGCCTTCCCC 
GCTTCGGCCG CCCTGGCCCC AGCCACTCCA GCCTTGCAGC CTATCCACAT TCAGCAGCCG 
GCCACGGCCT CTGCCACCTC CATCACAACT GTGCAGCATG CCATCCTACA GCATCATGCC 
GCAGCAGCTG CCGCTGCCAT TGGCATTCAC CCCCACCCTC ACCCCCAGCC ACTAGCCCAA 
GTGCATCATA TCCCCCAGCC CCACCTGACC CCCATCTCCT TGTCCCACCT CACTCACTCC 
ATCATCCCTG GTCATCCCGC CACCTTTCTG GCTAGCCACC CCATCCACAT CATTCCTGCC 
TCAGCCATCC ATCCCGGGCC CTTCACCTTC CACCCTGTTC CACACGCTGC CCTCTACCCT 
ACCCTGCTGG CTCCCCGGCC TGCTGCAGCA GCTGCCACTG CCCTCCACCT TCACCCACTA 
CTTCACCCCA TCTTCTCAGG TCAGGACCTG CAGCACCCCC CCAGCCATGG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323670.1
CDS	176..4792
Translation	MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_015468184.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468184.1

ORF Insert Sequence:

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGGCAGGCAC ACAGGATTAT TATGAATCTG AGATAATAGC TGATGTATTG 
AAAGCAAATC CTTCTAATTT GGAAGCCCAG ATCACAAGAA GATTGAAAGA TCTCAAGCAG 
AGAGAGTTTG CTCGAAATGT CTCCTCAAGA TCCCGCAAGG ATGAGAAGAA ACAGGAGAAA 
GCCCTACGGC GGCTCCACGA GTTGGCAGAG CAAAGAAAAC AAGCTGAATG TGCACCTGGA 
AGTGGTCCCA TGTTCAGACC AACCACAGTG GCTGTAGATG AAGAAGCTGG AGATGATGAG 
AAGGATGAAT CAGCAACAAA CAGTGGCACA AGTACCGCTG CCGCTTGTGG CCTGGGATCT 
GAATTCTCCA CGGATAAAGG AGGCCCATTC ACTGCAGTAC AAATCACTAA TACCACTGGG 
CTGGCACAGG CTCCTGGGCC AGCCTCTCAA GGCATCAGCT TTGGCATTAA GAATAATCTG 
GGGACCCCAC TACAAAAATT GGGAGTGTCA TTTTCCTTTG CCAAGAAGGC TCCTGTCAAA 
CTCGAATCAA TAGCATCAGT TTTCAAGGAC CACGCAGAGG AAGGGACCTC TGATGATGGA 
ACAAAAGCTG ATGAAAAGGG TGCAGACCAA GGACTGCAGA AGGTGGGAGA TTCTGATGGT 
AGCAGTAATC TCGATGGTAA AAAAGAGGAT GAAGACCCTC AGGACGGAGG GTCCCTTGCC 
TCAACATTAT CTAAGTTAAA AAGAATGAAG CGAGAAGAAG GAGCTGGGGC TACAGAGCCA 
GAGTATTACC ACTACATCCC CCCAGCACAC TGCAAAGTAA AACCTAATTT TCCTTTTCTG 
CTCTTTATGA GAGCCAGTGA ACAAATGGAA GGTGATAATA GTACACACCC AAAGAATGCC 
CTAGACAGCA AAAAAAGTAG TTCTCCCAAG CCTAAAGGCT GCATCAAGGT GACAGCAAGC 
CAAGGACCGG AAAAGACAGT CAGTGAAGTC TCCGAACAGC AAATGGAAAC CAGTGCGGCT 
GAGCCCTCAG AGCCTGGAAG CAAAGCTGAG ACAAAGAAGC CCTCAGGAGG GGATGTAAGT 
GAGCATAGTT TAGAGATTCA GAGTCAGAAG GATTCAGAGA TCCAAATGTG TGAGTCTGAC 
CCTCATAAAG AAACTTCTCA GGCTACTCCA GCAGGGAGAG AAAGCCGGGA GGGACCCAAA 
CATCCAACTG GTCCTTTCTT TCCGGTTTTA AGCAAAGATG AAAGCACTGC CCTCCAGTGG 
CCATCAGAAC TGTTAATTTT CACGAAGGCA GAACCCTCCA TTTCGTACAG TTGTAACCCT 
TTGTATTTTG ACTTCAAGCT TTCAAGGAAC AAAGATGCCA GAGCAAAAGG GACAGAAAAG 
CCAAAGGACA TAGGAGGCTC CTCAAAGGAC CATCTCCAAG GCCTTGATCC TAGTGAGCTA 
CATAAAAGCC AGGAGGAGGA AGAGAGTGTT CAACATTCTT CAGGAGGCAG AATCGATGCA 
CCTGCTTCAG GGGCTGCCTG TAGCGGCCTA AATAAGCAGG AGCCTGGGGG TAGCCATGGA 
TCAGAGACAG AAGACACAGG GAGAAGCCTT CCTAGCAAGA AAGAACGATC TGGGAAGTCC 
CACCGACACA AAAAGAAAAA GAAGCACAAA AAATCCAGCA AACATAAACG GAAACACAGG 
GCTGACCCAG AAGAGAAAAG CTCTAAGGCA GAATCTGGGG AGAAGTCTAA GAAGCGCAAG 
AAGCGAAAAC GAAAAAAGAA TAAATCATCA GCCCCAGCAG ATTCTGAACG GGGGCCCAAA 
CCAGAACCCC CTGGTAGTGG TAGCCCTGCA CCACCAAGAA GACGGCGGCG AGCCCAGGAT 
GATTCGCAGC GGAGATCCCT TCCAGCTGAG GAAGGGAGCA GCGGCAAGAA GGATGAAGGC 
GGAGGCAGTG GGAGCGCCCA AGATCATAGT GGGAGGAAAC ACAGGGGTGA ACCTCCCGCA 
TCATCCTGCC AGCGAAGAGC TGGCACCAAG CGGAGCAGCC GATCCAGCCA TCGAAGTCGA 
CCCAGTAGTG GAGATGAGGA CAGTGATGAT GCATCATCAC GCCGGCTGCA CCAGAAGTCT 
CCATCCCAGT ACAGTGAGGA GGAGGAGGAG GAGGAGGAGG AAGAGGAAGA GTCGGGCAGT 
GAGCATTCCC GAAGCCGCTC ACGGTCTGGC CGGCACCATT CCTCTCGCCA TTCCTCCCGG 
CGCTCTTACT CCAGTAGCTC AGATGCTTCT TCAGACCAGA GCTGCTATAG TAGACAACAC 
AGTTACTCTG ATGACAGCTA TAGTGACTAT AGTGACCGGT CACGAAGGCA CTCCAAGCGC 
TCCCATGACT CTGATGACTC AGACTACGCC AGCTCCAAGC ACCGGTCCAA GCGGCACAAA 
TATTCATCTT CTGATGATGA CTATAGCCTC AGTGGCAGCC AGTCCCGAAG CCGATCTCGG 
AGTCATACCA GAGAGCGCTC AAGATCCAGG GGCCGTAGCC GCAGCAGCAG CTGTAGTCGC 
AGCCGGAGCA AAAGGAGAAG CCGCAGCACC ACAGCCCACA GCTGGCAGCG GAGTCGGAGC 
TATAGCCGGG ACCGCAGCCG CAGCACCCGG AGCCCTTCAC AGAGATCAGG CTCCAGGAAG 
GGATCGTGGG GGCACGAGAG CCCTGAAGAG AGGCGTTCTG GTCGTCGAGA CTTCATTCGC 
TCTAAAATCT ACCGCTCCCA GTCTCCCCAT TACTTCCGAT CAGGCCGGGG AGAAGGTCCT 
GGGGAGAAGA AGAAAGAAGA CGGCAGAGGA GATGATGGTA AGGGAATAGG CCCACCCTCC 
CAGAACAGCA GCGTTGGCCC AGGAAGAGGG TCAGAAGGTG ACTGCAGCCC TGAAGACAAG 
AACTCTGTCA CTGCCAAGCT GCTGCTGGAA AAGATTCAGT CAAGGAAAGT GGAGAGGAAA 
CCCAGTATGA GTGAGGAGGT GCTGGCCACC CCTAATAAAG CCGGTCTCAA GCTCAAGGAC 
CCCCCACAAG GTTATTTTGG ACCCAAGCTC CCCCCTTCTC TTGGCAACAA GCCTGTCCTT 
CCTCTGATAG GGAAGCTCCC AGCTACCCGA AAGCCCAACG CCAAGAAATG TGAAGAGTCT 
GGCTTAGAAA GGGGAGAAGA GCAAGAACAG TCAGAGACAG AAGAAGGGCC TCCAGGGAAT 
AGTGATGCTC CATTTGGACA TCAGTTCCCC TCAGAGGAGA CAGCTGGCCC CTTATCAGAC 
CCACCCCCAG AAGAGCCAAA GTCTGAAGAA GCTACTGCTG ATCACCCTGT GGCTCCACTA 
GGCACCCCAG TGCACTCTGA CTGCTATCCT GGGGACCCAT CCATCTCCCA TAACTACCTC 
CCTGACCCCA GCGACGGGGA CACCCTTGAG TCCCTGGATA GTGGCAGTCA GCCAGGCCCC 
GTGGAGTCCA GCTTGCTGCC TATAGCATCA GACCTCGAGC CCTTCCCCGG TTACGCACCG 
CCCAGTGGGG AACCCAGTAT TGAGTCAGTT GATGGAGCCG AGGATGCATC CCTCGCCCCA 
CTGGAGAGCC AGCCCATCAC CTTCACTCCC GAGGAGATGG AGAAGTACAG CAAGCTCCAG 
CAGGCCGCAC AGCAGCACAT CCAGCAGCAG CTTCTGGCCA AGCAGGTGAA GGCCTTCCCC 
GCTTCGGCCG CCCTGGCCCC AGCCACTCCA GCCTTGCAGC CTATCCACAT TCAGCAGCCG 
GCCACGGCCT CTGCCACCTC CATCACAACT GTGCAGCATG CCATCCTACA GCATCATGCC 
GCAGCAGCTG CCGCTGCCAT TGGCATTCAC CCCCACCCTC ACCCCCAGCC ACTAGCCCAA 
GTGCATCATA TCCCCCAGCC CCACCTGACC CCCATCTCCT TGTCCCACCT CACTCACTCC 
ATCATCCCTG GTCATCCCGC CACCTTTCTG GCTAGCCACC CCATCCACAT CATTCCTGCC 
TCAGCCATCC ATCCCGGGCC CTTCACCTTC CACCCTGTTC CACACGCTGC CCTCTACCCT 
ACCCTGCTGG CTCCCCGGCC TGCTGCAGCA GCTGCCACTG CCCTCCACCT TCACCCACTA 
CTTCACCCCA TCTTCTCAGG TCAGGACCTG CAGCACCCCC CCAGCCATGG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_015458900.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314386.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93991
Clone ID Related Accession (Same CDS sequence)	XM_015468184.1 , XM_015458900.1
Accession Version	XM_015458900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGGCAGGCAC ACAGGATTAT TATGAATCTG AGATAATAGC TGATGTATTG 
AAAGCAAATC CTTCTAATTT GGAAGCCCAG ATCACAAGAA GATTGAAAGA TCTCAAGCAG 
AGAGAGTTTG CTCGAAATGT CTCCTCAAGA TCCCGCAAGG ATGAGAAGAA ACAGGAGAAA 
GCCCTACGGC GGCTCCACGA GTTGGCAGAG CAAAGAAAAC AAGCTGAATG TGCACCTGGA 
AGTGGTCCCA TGTTCAGACC AACCACAGTG GCTGTAGATG AAGAAGCTGG AGATGATGAG 
AAGGATGAAT CAGCAACAAA CAGTGGCACA AGTACCGCTG CCGCTTGTGG CCTGGGATCT 
GAATTCTCCA CGGATAAAGG AGGCCCATTC ACTGCAGTAC AAATCACTAA TACCACTGGG 
CTGGCACAGG CTCCTGGGCC AGCCTCTCAA GGCATCAGCT TTGGCATTAA GAATAATCTG 
GGGACCCCAC TACAAAAATT GGGAGTGTCA TTTTCCTTTG CCAAGAAGGC TCCTGTCAAA 
CTCGAATCAA TAGCATCAGT TTTCAAGGAC CACGCAGAGG AAGGGACCTC TGATGATGGA 
ACAAAAGCTG ATGAAAAGGG TGCAGACCAA GGACTGCAGA AGGTGGGAGA TTCTGATGGT 
AGCAGTAATC TCGATGGTAA AAAAGAGGAT GAAGACCCTC AGGACGGAGG GTCCCTTGCC 
TCAACATTAT CTAAGTTAAA AAGAATGAAG CGAGAAGAAG GAGCTGGGGC TACAGAGCCA 
GAGTATTACC ACTACATCCC CCCAGCACAC TGCAAAGTAA AACCTAATTT TCCTTTTCTG 
CTCTTTATGA GAGCCAGTGA ACAAATGGAA GGTGATAATA GTACACACCC AAAGAATGCC 
CTAGACAGCA AAAAAAGTAG TTCTCCCAAG CCTAAAGGCT GCATCAAGGT GACAGCAAGC 
CAAGGACCGG AAAAGACAGT CAGTGAAGTC TCCGAACAGC AAATGGAAAC CAGTGCGGCT 
GAGCCCTCAG AGCCTGGAAG CAAAGCTGAG ACAAAGAAGC CCTCAGGAGG GGATGTAAGT 
GAGCATAGTT TAGAGATTCA GAGTCAGAAG GATTCAGAGA TCCAAATGTG TGAGTCTGAC 
CCTCATAAAG AAACTTCTCA GGCTACTCCA GCAGGGAGAG AAAGCCGGGA GGGACCCAAA 
CATCCAACTG GTCCTTTCTT TCCGGTTTTA AGCAAAGATG AAAGCACTGC CCTCCAGTGG 
CCATCAGAAC TGTTAATTTT CACGAAGGCA GAACCCTCCA TTTCGTACAG TTGTAACCCT 
TTGTATTTTG ACTTCAAGCT TTCAAGGAAC AAAGATGCCA GAGCAAAAGG GACAGAAAAG 
CCAAAGGACA TAGGAGGCTC CTCAAAGGAC CATCTCCAAG GCCTTGATCC TAGTGAGCTA 
CATAAAAGCC AGGAGGAGGA AGAGAGTGTT CAACATTCTT CAGGAGGCAG AATCGATGCA 
CCTGCTTCAG GGGCTGCCTG TAGCGGCCTA AATAAGCAGG AGCCTGGGGG TAGCCATGGA 
TCAGAGACAG AAGACACAGG GAGAAGCCTT CCTAGCAAGA AAGAACGATC TGGGAAGTCC 
CACCGACACA AAAAGAAAAA GAAGCACAAA AAATCCAGCA AACATAAACG GAAACACAGG 
GCTGACCCAG AAGAGAAAAG CTCTAAGGCA GAATCTGGGG AGAAGTCTAA GAAGCGCAAG 
AAGCGAAAAC GAAAAAAGAA TAAATCATCA GCCCCAGCAG ATTCTGAACG GGGGCCCAAA 
CCAGAACCCC CTGGTAGTGG TAGCCCTGCA CCACCAAGAA GACGGCGGCG AGCCCAGGAT 
GATTCGCAGC GGAGATCCCT TCCAGCTGAG GAAGGGAGCA GCGGCAAGAA GGATGAAGGC 
GGAGGCAGTG GGAGCGCCCA AGATCATAGT GGGAGGAAAC ACAGGGGTGA ACCTCCCGCA 
TCATCCTGCC AGCGAAGAGC TGGCACCAAG CGGAGCAGCC GATCCAGCCA TCGAAGTCGA 
CCCAGTAGTG GAGATGAGGA CAGTGATGAT GCATCATCAC GCCGGCTGCA CCAGAAGTCT 
CCATCCCAGT ACAGTGAGGA GGAGGAGGAG GAGGAGGAGG AAGAGGAAGA GTCGGGCAGT 
GAGCATTCCC GAAGCCGCTC ACGGTCTGGC CGGCACCATT CCTCTCGCCA TTCCTCCCGG 
CGCTCTTACT CCAGTAGCTC AGATGCTTCT TCAGACCAGA GCTGCTATAG TAGACAACAC 
AGTTACTCTG ATGACAGCTA TAGTGACTAT AGTGACCGGT CACGAAGGCA CTCCAAGCGC 
TCCCATGACT CTGATGACTC AGACTACGCC AGCTCCAAGC ACCGGTCCAA GCGGCACAAA 
TATTCATCTT CTGATGATGA CTATAGCCTC AGTGGCAGCC AGTCCCGAAG CCGATCTCGG 
AGTCATACCA GAGAGCGCTC AAGATCCAGG GGCCGTAGCC GCAGCAGCAG CTGTAGTCGC 
AGCCGGAGCA AAAGGAGAAG CCGCAGCACC ACAGCCCACA GCTGGCAGCG GAGTCGGAGC 
TATAGCCGGG ACCGCAGCCG CAGCACCCGG AGCCCTTCAC AGAGATCAGG CTCCAGGAAG 
GGATCGTGGG GGCACGAGAG CCCTGAAGAG AGGCGTTCTG GTCGTCGAGA CTTCATTCGC 
TCTAAAATCT ACCGCTCCCA GTCTCCCCAT TACTTCCGAT CAGGCCGGGG AGAAGGTCCT 
GGGGAGAAGA AGAAAGAAGA CGGCAGAGGA GATGATGGTA AGGGAATAGG CCCACCCTCC 
CAGAACAGCA GCGTTGGCCC AGGAAGAGGG TCAGAAGGTG ACTGCAGCCC TGAAGACAAG 
AACTCTGTCA CTGCCAAGCT GCTGCTGGAA AAGATTCAGT CAAGGAAAGT GGAGAGGAAA 
CCCAGTATGA GTGAGGAGGT GCTGGCCACC CCTAATAAAG CCGGTCTCAA GCTCAAGGAC 
CCCCCACAAG GTTATTTTGG ACCCAAGCTC CCCCCTTCTC TTGGCAACAA GCCTGTCCTT 
CCTCTGATAG GGAAGCTCCC AGCTACCCGA AAGCCCAACG CCAAGAAATG TGAAGAGTCT 
GGCTTAGAAA GGGGAGAAGA GCAAGAACAG TCAGAGACAG AAGAAGGGCC TCCAGGGAAT 
AGTGATGCTC CATTTGGACA TCAGTTCCCC TCAGAGGAGA CAGCTGGCCC CTTATCAGAC 
CCACCCCCAG AAGAGCCAAA GTCTGAAGAA GCTACTGCTG ATCACCCTGT GGCTCCACTA 
GGCACCCCAG TGCACTCTGA CTGCTATCCT GGGGACCCAT CCATCTCCCA TAACTACCTC 
CCTGACCCCA GCGACGGGGA CACCCTTGAG TCCCTGGATA GTGGCAGTCA GCCAGGCCCC 
GTGGAGTCCA GCTTGCTGCC TATAGCATCA GACCTCGAGC CCTTCCCCGG TTACGCACCG 
CCCAGTGGGG AACCCAGTAT TGAGTCAGTT GATGGAGCCG AGGATGCATC CCTCGCCCCA 
CTGGAGAGCC AGCCCATCAC CTTCACTCCC GAGGAGATGG AGAAGTACAG CAAGCTCCAG 
CAGGCCGCAC AGCAGCACAT CCAGCAGCAG CTTCTGGCCA AGCAGGTGAA GGCCTTCCCC 
GCTTCGGCCG CCCTGGCCCC AGCCACTCCA GCCTTGCAGC CTATCCACAT TCAGCAGCCG 
GCCACGGCCT CTGCCACCTC CATCACAACT GTGCAGCATG CCATCCTACA GCATCATGCC 
GCAGCAGCTG CCGCTGCCAT TGGCATTCAC CCCCACCCTC ACCCCCAGCC ACTAGCCCAA 
GTGCATCATA TCCCCCAGCC CCACCTGACC CCCATCTCCT TGTCCCACCT CACTCACTCC 
ATCATCCCTG GTCATCCCGC CACCTTTCTG GCTAGCCACC CCATCCACAT CATTCCTGCC 
TCAGCCATCC ATCCCGGGCC CTTCACCTTC CACCCTGTTC CACACGCTGC CCTCTACCCT 
ACCCTGCTGG CTCCCCGGCC TGCTGCAGCA GCTGCCACTG CCCTCCACCT TCACCCACTA 
CTTCACCCCA TCTTCTCAGG TCAGGACCTG CAGCACCCCC CCAGCCATGG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314386.1
CDS	176..4792
Translation	MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_015458900.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458900.1

ORF Insert Sequence:

ATGGCGGACC GCTTCTCCCG CTTCAACGAG GACCGAGACT TTCAGGGTAA TCACTTTGAT 
CAATATGAAG AAGGACATTT GGAAATTGAA CAAGCATCCC TTGACAAGCC TATAGAATCG 
GATAATATTG GACATCGCTT ACTCCAGAAA CATGGGTGGA AGCTGGGCCA GGGATTGGGA 
AAATCTCTTC AGGGGAGAAC AGATCCCATT CCAATCGTCG TCAAATATGA TGTCATGGGC 
ATGGGTCGCA TGGAAATGGA GCTTGATTAT GCTGAAGATG CTACCGAACG GCGCCGTGTC 
TTAGAAGTAG AAAAAGAAGA CACGGAAGAA CTGAGGCAGA AGTACAAGGA TTATGTTGAC 
AAAGAGAAGG CCATTGCCAA AGCACTGGAA GACCTTAGAG CCAACTTTTA CTGTGAACTC 
TGTGATAAGC AGTATCAGAA ACACCAGGAA TTTGACAACC ATATCAACTC CTATGATCAT 
GCACACAAGC AGGCAGGCAC ACAGGATTAT TATGAATCTG AGATAATAGC TGATGTATTG 
AAAGCAAATC CTTCTAATTT GGAAGCCCAG ATCACAAGAA GATTGAAAGA TCTCAAGCAG 
AGAGAGTTTG CTCGAAATGT CTCCTCAAGA TCCCGCAAGG ATGAGAAGAA ACAGGAGAAA 
GCCCTACGGC GGCTCCACGA GTTGGCAGAG CAAAGAAAAC AAGCTGAATG TGCACCTGGA 
AGTGGTCCCA TGTTCAGACC AACCACAGTG GCTGTAGATG AAGAAGCTGG AGATGATGAG 
AAGGATGAAT CAGCAACAAA CAGTGGCACA AGTACCGCTG CCGCTTGTGG CCTGGGATCT 
GAATTCTCCA CGGATAAAGG AGGCCCATTC ACTGCAGTAC AAATCACTAA TACCACTGGG 
CTGGCACAGG CTCCTGGGCC AGCCTCTCAA GGCATCAGCT TTGGCATTAA GAATAATCTG 
GGGACCCCAC TACAAAAATT GGGAGTGTCA TTTTCCTTTG CCAAGAAGGC TCCTGTCAAA 
CTCGAATCAA TAGCATCAGT TTTCAAGGAC CACGCAGAGG AAGGGACCTC TGATGATGGA 
ACAAAAGCTG ATGAAAAGGG TGCAGACCAA GGACTGCAGA AGGTGGGAGA TTCTGATGGT 
AGCAGTAATC TCGATGGTAA AAAAGAGGAT GAAGACCCTC AGGACGGAGG GTCCCTTGCC 
TCAACATTAT CTAAGTTAAA AAGAATGAAG CGAGAAGAAG GAGCTGGGGC TACAGAGCCA 
GAGTATTACC ACTACATCCC CCCAGCACAC TGCAAAGTAA AACCTAATTT TCCTTTTCTG 
CTCTTTATGA GAGCCAGTGA ACAAATGGAA GGTGATAATA GTACACACCC AAAGAATGCC 
CTAGACAGCA AAAAAAGTAG TTCTCCCAAG CCTAAAGGCT GCATCAAGGT GACAGCAAGC 
CAAGGACCGG AAAAGACAGT CAGTGAAGTC TCCGAACAGC AAATGGAAAC CAGTGCGGCT 
GAGCCCTCAG AGCCTGGAAG CAAAGCTGAG ACAAAGAAGC CCTCAGGAGG GGATGTAAGT 
GAGCATAGTT TAGAGATTCA GAGTCAGAAG GATTCAGAGA TCCAAATGTG TGAGTCTGAC 
CCTCATAAAG AAACTTCTCA GGCTACTCCA GCAGGGAGAG AAAGCCGGGA GGGACCCAAA 
CATCCAACTG GTCCTTTCTT TCCGGTTTTA AGCAAAGATG AAAGCACTGC CCTCCAGTGG 
CCATCAGAAC TGTTAATTTT CACGAAGGCA GAACCCTCCA TTTCGTACAG TTGTAACCCT 
TTGTATTTTG ACTTCAAGCT TTCAAGGAAC AAAGATGCCA GAGCAAAAGG GACAGAAAAG 
CCAAAGGACA TAGGAGGCTC CTCAAAGGAC CATCTCCAAG GCCTTGATCC TAGTGAGCTA 
CATAAAAGCC AGGAGGAGGA AGAGAGTGTT CAACATTCTT CAGGAGGCAG AATCGATGCA 
CCTGCTTCAG GGGCTGCCTG TAGCGGCCTA AATAAGCAGG AGCCTGGGGG TAGCCATGGA 
TCAGAGACAG AAGACACAGG GAGAAGCCTT CCTAGCAAGA AAGAACGATC TGGGAAGTCC 
CACCGACACA AAAAGAAAAA GAAGCACAAA AAATCCAGCA AACATAAACG GAAACACAGG 
GCTGACCCAG AAGAGAAAAG CTCTAAGGCA GAATCTGGGG AGAAGTCTAA GAAGCGCAAG 
AAGCGAAAAC GAAAAAAGAA TAAATCATCA GCCCCAGCAG ATTCTGAACG GGGGCCCAAA 
CCAGAACCCC CTGGTAGTGG TAGCCCTGCA CCACCAAGAA GACGGCGGCG AGCCCAGGAT 
GATTCGCAGC GGAGATCCCT TCCAGCTGAG GAAGGGAGCA GCGGCAAGAA GGATGAAGGC 
GGAGGCAGTG GGAGCGCCCA AGATCATAGT GGGAGGAAAC ACAGGGGTGA ACCTCCCGCA 
TCATCCTGCC AGCGAAGAGC TGGCACCAAG CGGAGCAGCC GATCCAGCCA TCGAAGTCGA 
CCCAGTAGTG GAGATGAGGA CAGTGATGAT GCATCATCAC GCCGGCTGCA CCAGAAGTCT 
CCATCCCAGT ACAGTGAGGA GGAGGAGGAG GAGGAGGAGG AAGAGGAAGA GTCGGGCAGT 
GAGCATTCCC GAAGCCGCTC ACGGTCTGGC CGGCACCATT CCTCTCGCCA TTCCTCCCGG 
CGCTCTTACT CCAGTAGCTC AGATGCTTCT TCAGACCAGA GCTGCTATAG TAGACAACAC 
AGTTACTCTG ATGACAGCTA TAGTGACTAT AGTGACCGGT CACGAAGGCA CTCCAAGCGC 
TCCCATGACT CTGATGACTC AGACTACGCC AGCTCCAAGC ACCGGTCCAA GCGGCACAAA 
TATTCATCTT CTGATGATGA CTATAGCCTC AGTGGCAGCC AGTCCCGAAG CCGATCTCGG 
AGTCATACCA GAGAGCGCTC AAGATCCAGG GGCCGTAGCC GCAGCAGCAG CTGTAGTCGC 
AGCCGGAGCA AAAGGAGAAG CCGCAGCACC ACAGCCCACA GCTGGCAGCG GAGTCGGAGC 
TATAGCCGGG ACCGCAGCCG CAGCACCCGG AGCCCTTCAC AGAGATCAGG CTCCAGGAAG 
GGATCGTGGG GGCACGAGAG CCCTGAAGAG AGGCGTTCTG GTCGTCGAGA CTTCATTCGC 
TCTAAAATCT ACCGCTCCCA GTCTCCCCAT TACTTCCGAT CAGGCCGGGG AGAAGGTCCT 
GGGGAGAAGA AGAAAGAAGA CGGCAGAGGA GATGATGGTA AGGGAATAGG CCCACCCTCC 
CAGAACAGCA GCGTTGGCCC AGGAAGAGGG TCAGAAGGTG ACTGCAGCCC TGAAGACAAG 
AACTCTGTCA CTGCCAAGCT GCTGCTGGAA AAGATTCAGT CAAGGAAAGT GGAGAGGAAA 
CCCAGTATGA GTGAGGAGGT GCTGGCCACC CCTAATAAAG CCGGTCTCAA GCTCAAGGAC 
CCCCCACAAG GTTATTTTGG ACCCAAGCTC CCCCCTTCTC TTGGCAACAA GCCTGTCCTT 
CCTCTGATAG GGAAGCTCCC AGCTACCCGA AAGCCCAACG CCAAGAAATG TGAAGAGTCT 
GGCTTAGAAA GGGGAGAAGA GCAAGAACAG TCAGAGACAG AAGAAGGGCC TCCAGGGAAT 
AGTGATGCTC CATTTGGACA TCAGTTCCCC TCAGAGGAGA CAGCTGGCCC CTTATCAGAC 
CCACCCCCAG AAGAGCCAAA GTCTGAAGAA GCTACTGCTG ATCACCCTGT GGCTCCACTA 
GGCACCCCAG TGCACTCTGA CTGCTATCCT GGGGACCCAT CCATCTCCCA TAACTACCTC 
CCTGACCCCA GCGACGGGGA CACCCTTGAG TCCCTGGATA GTGGCAGTCA GCCAGGCCCC 
GTGGAGTCCA GCTTGCTGCC TATAGCATCA GACCTCGAGC CCTTCCCCGG TTACGCACCG 
CCCAGTGGGG AACCCAGTAT TGAGTCAGTT GATGGAGCCG AGGATGCATC CCTCGCCCCA 
CTGGAGAGCC AGCCCATCAC CTTCACTCCC GAGGAGATGG AGAAGTACAG CAAGCTCCAG 
CAGGCCGCAC AGCAGCACAT CCAGCAGCAG CTTCTGGCCA AGCAGGTGAA GGCCTTCCCC 
GCTTCGGCCG CCCTGGCCCC AGCCACTCCA GCCTTGCAGC CTATCCACAT TCAGCAGCCG 
GCCACGGCCT CTGCCACCTC CATCACAACT GTGCAGCATG CCATCCTACA GCATCATGCC 
GCAGCAGCTG CCGCTGCCAT TGGCATTCAC CCCCACCCTC ACCCCCAGCC ACTAGCCCAA 
GTGCATCATA TCCCCCAGCC CCACCTGACC CCCATCTCCT TGTCCCACCT CACTCACTCC 
ATCATCCCTG GTCATCCCGC CACCTTTCTG GCTAGCCACC CCATCCACAT CATTCCTGCC 
TCAGCCATCC ATCCCGGGCC CTTCACCTTC CACCCTGTTC CACACGCTGC CCTCTACCCT 
ACCCTGCTGG CTCCCCGGCC TGCTGCAGCA GCTGCCACTG CCCTCCACCT TCACCCACTA 
CTTCACCCCA TCTTCTCAGG TCAGGACCTG CAGCACCCCC CCAGCCATGG CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010816406.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814708.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010816406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814708.1
CDS	214..4596
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010816406.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816406.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010816408.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814710.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

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CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010816408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814710.1
CDS	259..4641
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010816408.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816408.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010816409.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814711.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

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CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010816409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814711.1
CDS	123..4505
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010816409.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816409.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010824215.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822517.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010824215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822517.2
CDS	214..4596
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010824215.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824215.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010824219.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822521.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010824219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822521.2
CDS	123..4505
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010824219.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824219.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010824217.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822519.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

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CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010824217.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824217.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822519.2
CDS	259..4641
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010824217.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824217.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010824216.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822518.2 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

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CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010824216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822518.2
CDS	249..4631
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010824216.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824216.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_010816407.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814709.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_010816407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816407.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814709.1
CDS	249..4631
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_010816407.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816407.2

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_015458901.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314387.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_015458901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314387.1
CDS	177..4559
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_015458901.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458901.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH8 ( XM_015468185.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323671.1 Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

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CloneID	OBa52927
Clone ID Related Accession (Same CDS sequence)	XM_010816406.2 , XM_010816408.2 , XM_010816409.2 , XM_010824215.2 , XM_010824219.2 , XM_010824217.2 , XM_010824216.2 , XM_010816407.2 , XM_015458901.1 , XM_015468185.1
Accession Version	XM_015468185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G patch domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323671.1
CDS	177..4559
Translation	MGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQAGTQDYYESEIIADVLKANPSNLEAQITRRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFRPTTVAVDEEAGDDEKDESATNSGTSTAAACGLGSEFSTDKGGPFTAVQITNTTGLAQAPGPASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSDDGTKADEKGADQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMEGDNSTHPKNALDSKKSSSPKPKGCIKVTASQGPEKTVSEVSEQQMETSAAEPSEPGSKAETKKPSGGDVSEHSLEIQSQKDSEIQMCESDPHKETSQATPAGRESREGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARAKGTEKPKDIGGSSKDHLQGLDPSELHKSQEEEESVQHSSGGRIDAPASGAACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHRADPEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGSGSAQDHSGRKHRGEPPASSCQRRAGTKRSSRSSHRSRPSSGDEDSDDASSRRLHQKSPSQYSEEEEEEEEEEEESGSEHSRSRSRSGRHHSSRHSSRRSYSSSSDASSDQSCYSRQHSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSGSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKGSWGHESPEERRSGRRDFIRSKIYRSQSPHYFRSGRGEGPGEKKKEDGRGDDGKGIGPPSQNSSVGPGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSMSEEVLATPNKAGLKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNAKKCEESGLERGEEQEQSETEEGPPGNSDAPFGHQFPSEETAGPLSDPPPEEPKSEEATADHPVAPLGTPVHSDCYPGDPSISHNYLPDPSDGDTLESLDSGSQPGPVESSLLPIASDLEPFPGYAPPSGEPSIESVDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Target ORF information:

RefSeq Version	XM_015468185.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G-patch domain containing 8 (GPATCH8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468185.1

ORF Insert Sequence:

ATGGGCATGG GTCGCATGGA AATGGAGCTT GATTATGCTG AAGATGCTAC CGAACGGCGC 
CGTGTCTTAG AAGTAGAAAA AGAAGACACG GAAGAACTGA GGCAGAAGTA CAAGGATTAT 
GTTGACAAAG AGAAGGCCAT TGCCAAAGCA CTGGAAGACC TTAGAGCCAA CTTTTACTGT 
GAACTCTGTG ATAAGCAGTA TCAGAAACAC CAGGAATTTG ACAACCATAT CAACTCCTAT 
GATCATGCAC ACAAGCAGGC AGGCACACAG GATTATTATG AATCTGAGAT AATAGCTGAT 
GTATTGAAAG CAAATCCTTC TAATTTGGAA GCCCAGATCA CAAGAAGATT GAAAGATCTC 
AAGCAGAGAG AGTTTGCTCG AAATGTCTCC TCAAGATCCC GCAAGGATGA GAAGAAACAG 
GAGAAAGCCC TACGGCGGCT CCACGAGTTG GCAGAGCAAA GAAAACAAGC TGAATGTGCA 
CCTGGAAGTG GTCCCATGTT CAGACCAACC ACAGTGGCTG TAGATGAAGA AGCTGGAGAT 
GATGAGAAGG ATGAATCAGC AACAAACAGT GGCACAAGTA CCGCTGCCGC TTGTGGCCTG 
GGATCTGAAT TCTCCACGGA TAAAGGAGGC CCATTCACTG CAGTACAAAT CACTAATACC 
ACTGGGCTGG CACAGGCTCC TGGGCCAGCC TCTCAAGGCA TCAGCTTTGG CATTAAGAAT 
AATCTGGGGA CCCCACTACA AAAATTGGGA GTGTCATTTT CCTTTGCCAA GAAGGCTCCT 
GTCAAACTCG AATCAATAGC ATCAGTTTTC AAGGACCACG CAGAGGAAGG GACCTCTGAT 
GATGGAACAA AAGCTGATGA AAAGGGTGCA GACCAAGGAC TGCAGAAGGT GGGAGATTCT 
GATGGTAGCA GTAATCTCGA TGGTAAAAAA GAGGATGAAG ACCCTCAGGA CGGAGGGTCC 
CTTGCCTCAA CATTATCTAA GTTAAAAAGA ATGAAGCGAG AAGAAGGAGC TGGGGCTACA 
GAGCCAGAGT ATTACCACTA CATCCCCCCA GCACACTGCA AAGTAAAACC TAATTTTCCT 
TTTCTGCTCT TTATGAGAGC CAGTGAACAA ATGGAAGGTG ATAATAGTAC ACACCCAAAG 
AATGCCCTAG ACAGCAAAAA AAGTAGTTCT CCCAAGCCTA AAGGCTGCAT CAAGGTGACA 
GCAAGCCAAG GACCGGAAAA GACAGTCAGT GAAGTCTCCG AACAGCAAAT GGAAACCAGT 
GCGGCTGAGC CCTCAGAGCC TGGAAGCAAA GCTGAGACAA AGAAGCCCTC AGGAGGGGAT 
GTAAGTGAGC ATAGTTTAGA GATTCAGAGT CAGAAGGATT CAGAGATCCA AATGTGTGAG 
TCTGACCCTC ATAAAGAAAC TTCTCAGGCT ACTCCAGCAG GGAGAGAAAG CCGGGAGGGA 
CCCAAACATC CAACTGGTCC TTTCTTTCCG GTTTTAAGCA AAGATGAAAG CACTGCCCTC 
CAGTGGCCAT CAGAACTGTT AATTTTCACG AAGGCAGAAC CCTCCATTTC GTACAGTTGT 
AACCCTTTGT ATTTTGACTT CAAGCTTTCA AGGAACAAAG ATGCCAGAGC AAAAGGGACA 
GAAAAGCCAA AGGACATAGG AGGCTCCTCA AAGGACCATC TCCAAGGCCT TGATCCTAGT 
GAGCTACATA AAAGCCAGGA GGAGGAAGAG AGTGTTCAAC ATTCTTCAGG AGGCAGAATC 
GATGCACCTG CTTCAGGGGC TGCCTGTAGC GGCCTAAATA AGCAGGAGCC TGGGGGTAGC 
CATGGATCAG AGACAGAAGA CACAGGGAGA AGCCTTCCTA GCAAGAAAGA ACGATCTGGG 
AAGTCCCACC GACACAAAAA GAAAAAGAAG CACAAAAAAT CCAGCAAACA TAAACGGAAA 
CACAGGGCTG ACCCAGAAGA GAAAAGCTCT AAGGCAGAAT CTGGGGAGAA GTCTAAGAAG 
CGCAAGAAGC GAAAACGAAA AAAGAATAAA TCATCAGCCC CAGCAGATTC TGAACGGGGG 
CCCAAACCAG AACCCCCTGG TAGTGGTAGC CCTGCACCAC CAAGAAGACG GCGGCGAGCC 
CAGGATGATT CGCAGCGGAG ATCCCTTCCA GCTGAGGAAG GGAGCAGCGG CAAGAAGGAT 
GAAGGCGGAG GCAGTGGGAG CGCCCAAGAT CATAGTGGGA GGAAACACAG GGGTGAACCT 
CCCGCATCAT CCTGCCAGCG AAGAGCTGGC ACCAAGCGGA GCAGCCGATC CAGCCATCGA 
AGTCGACCCA GTAGTGGAGA TGAGGACAGT GATGATGCAT CATCACGCCG GCTGCACCAG 
AAGTCTCCAT CCCAGTACAG TGAGGAGGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGTCG 
GGCAGTGAGC ATTCCCGAAG CCGCTCACGG TCTGGCCGGC ACCATTCCTC TCGCCATTCC 
TCCCGGCGCT CTTACTCCAG TAGCTCAGAT GCTTCTTCAG ACCAGAGCTG CTATAGTAGA 
CAACACAGTT ACTCTGATGA CAGCTATAGT GACTATAGTG ACCGGTCACG AAGGCACTCC 
AAGCGCTCCC ATGACTCTGA TGACTCAGAC TACGCCAGCT CCAAGCACCG GTCCAAGCGG 
CACAAATATT CATCTTCTGA TGATGACTAT AGCCTCAGTG GCAGCCAGTC CCGAAGCCGA 
TCTCGGAGTC ATACCAGAGA GCGCTCAAGA TCCAGGGGCC GTAGCCGCAG CAGCAGCTGT 
AGTCGCAGCC GGAGCAAAAG GAGAAGCCGC AGCACCACAG CCCACAGCTG GCAGCGGAGT 
CGGAGCTATA GCCGGGACCG CAGCCGCAGC ACCCGGAGCC CTTCACAGAG ATCAGGCTCC 
AGGAAGGGAT CGTGGGGGCA CGAGAGCCCT GAAGAGAGGC GTTCTGGTCG TCGAGACTTC 
ATTCGCTCTA AAATCTACCG CTCCCAGTCT CCCCATTACT TCCGATCAGG CCGGGGAGAA 
GGTCCTGGGG AGAAGAAGAA AGAAGACGGC AGAGGAGATG ATGGTAAGGG AATAGGCCCA 
CCCTCCCAGA ACAGCAGCGT TGGCCCAGGA AGAGGGTCAG AAGGTGACTG CAGCCCTGAA 
GACAAGAACT CTGTCACTGC CAAGCTGCTG CTGGAAAAGA TTCAGTCAAG GAAAGTGGAG 
AGGAAACCCA GTATGAGTGA GGAGGTGCTG GCCACCCCTA ATAAAGCCGG TCTCAAGCTC 
AAGGACCCCC CACAAGGTTA TTTTGGACCC AAGCTCCCCC CTTCTCTTGG CAACAAGCCT 
GTCCTTCCTC TGATAGGGAA GCTCCCAGCT ACCCGAAAGC CCAACGCCAA GAAATGTGAA 
GAGTCTGGCT TAGAAAGGGG AGAAGAGCAA GAACAGTCAG AGACAGAAGA AGGGCCTCCA 
GGGAATAGTG ATGCTCCATT TGGACATCAG TTCCCCTCAG AGGAGACAGC TGGCCCCTTA 
TCAGACCCAC CCCCAGAAGA GCCAAAGTCT GAAGAAGCTA CTGCTGATCA CCCTGTGGCT 
CCACTAGGCA CCCCAGTGCA CTCTGACTGC TATCCTGGGG ACCCATCCAT CTCCCATAAC 
TACCTCCCTG ACCCCAGCGA CGGGGACACC CTTGAGTCCC TGGATAGTGG CAGTCAGCCA 
GGCCCCGTGG AGTCCAGCTT GCTGCCTATA GCATCAGACC TCGAGCCCTT CCCCGGTTAC 
GCACCGCCCA GTGGGGAACC CAGTATTGAG TCAGTTGATG GAGCCGAGGA TGCATCCCTC 
GCCCCACTGG AGAGCCAGCC CATCACCTTC ACTCCCGAGG AGATGGAGAA GTACAGCAAG 
CTCCAGCAGG CCGCACAGCA GCACATCCAG CAGCAGCTTC TGGCCAAGCA GGTGAAGGCC 
TTCCCCGCTT CGGCCGCCCT GGCCCCAGCC ACTCCAGCCT TGCAGCCTAT CCACATTCAG 
CAGCCGGCCA CGGCCTCTGC CACCTCCATC ACAACTGTGC AGCATGCCAT CCTACAGCAT 
CATGCCGCAG CAGCTGCCGC TGCCATTGGC ATTCACCCCC ACCCTCACCC CCAGCCACTA 
GCCCAAGTGC ATCATATCCC CCAGCCCCAC CTGACCCCCA TCTCCTTGTC CCACCTCACT 
CACTCCATCA TCCCTGGTCA TCCCGCCACC TTTCTGGCTA GCCACCCCAT CCACATCATT 
CCTGCCTCAG CCATCCATCC CGGGCCCTTC ACCTTCCACC CTGTTCCACA CGCTGCCCTC 
TACCCTACCC TGCTGGCTCC CCGGCCTGCT GCAGCAGCTG CCACTGCCCT CCACCTTCAC 
CCACTACTTC ACCCCATCTT CTCAGGTCAG GACCTGCAGC ACCCCCCCAG CCATGGCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GPATCH8
Entrez Gene ID	534759
Full Name	G-patch domain containing 8
General protein information	Preferred Names G-patch domain containing 8 Names G patch domain-containing protein 8 G patch domain-containing protein 8 pseudogene
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	GPATCH8	XP_005221017.1
Canis lupus familiaris (dog)	GPATCH8	XP_537619.2
Homo sapiens (human)	GPATCH8	NP_001002909.1
Pan troglodytes (chimpanzee)	GPATCH8	XP_001150301.1
	GPATCH8	XP_001114589.1
Mus musculus (house mouse)	Gpatch8	NP_001152964.1
Rattus norvegicus (Norway rat)	Gpatch8	XP_001062937.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GPATCH8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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