MID1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	534604
Full Name	midline 1
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005228472.4	XP_005228529.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_005228478.4	XP_005228535.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
NM_001192822.1	NP_001179751.1	E3 ubiquitin-protein ligase Midline-1
XM_005228478.3	XP_005228535.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_005228472.3	XP_005228529.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_005228477.3	XP_005228534.1	E3 ubiquitin-protein ligase Midline-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Widely spaced alternative promoters, conserved between human and rodent, control expression of the Opitz syndrome gene MID1.
Landry JR, Mager DL
Genomics80(5)499-508(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa35831	XM_005228478.4 Latest version!	Bos taurus midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa35830	NM_001192822.1 Latest version!	Bos taurus midline 1 (MID1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa35830	XM_005228477.3 Latest version!	Bos taurus midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa35830	XM_005228472.4 Latest version!	Bos taurus midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa35831	XM_005228478.3	Bos taurus midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa35830	XM_005228472.3	Bos taurus midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( XM_005228478.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228535.1 Bos taurus midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35831
Clone ID Related Accession (Same CDS sequence)	XM_005228478.4 , XM_005228478.3
Accession Version	XM_005228478.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228478.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGCAAA ACTTGGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC GGAACTGGAG 
ACGCTTTTGG CTAAACTCAT TCAAACATGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA ATGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATCATTGGAA CCAAGATCAA AGAAGGAAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATCGAGCGG TCGGCATCAC TCATCTCCCA AGCAGAACAC 
TCTCTGAAGG AGAATGATCA CGCGCGTTTC CTACAGACCG CTAAGAATAT CACTGAGAGA 
GTCTCCATGG CAACAGCATC ATCCCAGGTC CTCATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TCGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACGGCTTC CTATGACACC 
ATAACCGTTC ACTGGACCTC TGATGACGAA TTCAGTGTGG TTTCCTATGA GCTCCAGTAC 
ACCATCTTCA CTGGACAAGC CAATGTTGTG AGTCTGTGCA ACTCGGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCATG GGCTGCAGAG CGGCACCAAG 
TACATCTTCA TTGTCAAGGC CATCAACCAG GCAGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACGA ACAGCCAACC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTAAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGCCACACG 
CCTGAGCGCT TCACCAGCCA AGGCAGCTAT GGCGTAGCTG GAAACGTGTT CATTGATAGT 
GGCCGCCATT ACTGGGAAGT GGTCATAAGC GGAAGCACAT GGTATGCCAT TGGCCTTGCA 
TACAAGTCAG CCCCAAAGCA CGAGTGGATT GGCAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGCCACA ATACCTGGGT GGTAAGACAC AACAGCAAGG AGATCCCCAT CGAGCCAGCC 
CCCCACCTCC GGCGTGTCGG CATTCTGCTA GACTACGATA ATGGCTCCAT CGCTTTCTAT 
GATGCCTTGA ACTCCATCCA CCTCTACACT TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACCTTCA CTGTGTGGAA CAAATGTCTG ACCATCATAA CAGGCCTTCC CATCCCTGAC 
CATCTAGACT GTACAGAGCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228535.1
CDS	215..2104
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005228478.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228478.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGCAAA ACTTGGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC GGAACTGGAG 
ACGCTTTTGG CTAAACTCAT TCAAACATGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA ATGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATCATTGGAA CCAAGATCAA AGAAGGAAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATCGAGCGG TCGGCATCAC TCATCTCCCA AGCAGAACAC 
TCTCTGAAGG AGAATGATCA CGCGCGTTTC CTACAGACCG CTAAGAATAT CACTGAGAGA 
GTCTCCATGG CAACAGCATC ATCCCAGGTC CTCATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TCGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACGGCTTC CTATGACACC 
ATAACCGTTC ACTGGACCTC TGATGACGAA TTCAGTGTGG TTTCCTATGA GCTCCAGTAC 
ACCATCTTCA CTGGACAAGC CAATGTTGTG AGTCTGTGCA ACTCGGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCATG GGCTGCAGAG CGGCACCAAG 
TACATCTTCA TTGTCAAGGC CATCAACCAG GCAGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACGA ACAGCCAACC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTAAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGCCACACG 
CCTGAGCGCT TCACCAGCCA AGGCAGCTAT GGCGTAGCTG GAAACGTGTT CATTGATAGT 
GGCCGCCATT ACTGGGAAGT GGTCATAAGC GGAAGCACAT GGTATGCCAT TGGCCTTGCA 
TACAAGTCAG CCCCAAAGCA CGAGTGGATT GGCAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGCCACA ATACCTGGGT GGTAAGACAC AACAGCAAGG AGATCCCCAT CGAGCCAGCC 
CCCCACCTCC GGCGTGTCGG CATTCTGCTA GACTACGATA ATGGCTCCAT CGCTTTCTAT 
GATGCCTTGA ACTCCATCCA CCTCTACACT TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACCTTCA CTGTGTGGAA CAAATGTCTG ACCATCATAA CAGGCCTTCC CATCCCTGAC 
CATCTAGACT GTACAGAGCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005228478.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228535.1 Bos taurus midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35831
Clone ID Related Accession (Same CDS sequence)	XM_005228478.4 , XM_005228478.3
Accession Version	XM_005228478.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104781.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGCAAA ACTTGGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC GGAACTGGAG 
ACGCTTTTGG CTAAACTCAT TCAAACATGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA ATGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATCATTGGAA CCAAGATCAA AGAAGGAAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATCGAGCGG TCGGCATCAC TCATCTCCCA AGCAGAACAC 
TCTCTGAAGG AGAATGATCA CGCGCGTTTC CTACAGACCG CTAAGAATAT CACTGAGAGA 
GTCTCCATGG CAACAGCATC ATCCCAGGTC CTCATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TCGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACGGCTTC CTATGACACC 
ATAACCGTTC ACTGGACCTC TGATGACGAA TTCAGTGTGG TTTCCTATGA GCTCCAGTAC 
ACCATCTTCA CTGGACAAGC CAATGTTGTG AGTCTGTGCA ACTCGGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCATG GGCTGCAGAG CGGCACCAAG 
TACATCTTCA TTGTCAAGGC CATCAACCAG GCAGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACGA ACAGCCAACC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTAAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGCCACACG 
CCTGAGCGCT TCACCAGCCA AGGCAGCTAT GGCGTAGCTG GAAACGTGTT CATTGATAGT 
GGCCGCCATT ACTGGGAAGT GGTCATAAGC GGAAGCACAT GGTATGCCAT TGGCCTTGCA 
TACAAGTCAG CCCCAAAGCA CGAGTGGATT GGCAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGCCACA ATACCTGGGT GGTAAGACAC AACAGCAAGG AGATCCCCAT CGAGCCAGCC 
CCCCACCTCC GGCGTGTCGG CATTCTGCTA GACTACGATA ATGGCTCCAT CGCTTTCTAT 
GATGCCTTGA ACTCCATCCA CCTCTACACT TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACCTTCA CTGTGTGGAA CAAATGTCTG ACCATCATAA CAGGCCTTCC CATCCCTGAC 
CATCTAGACT GTACAGAGCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228535.1
CDS	212..2101
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005228478.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228478.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGCAAA ACTTGGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC GGAACTGGAG 
ACGCTTTTGG CTAAACTCAT TCAAACATGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA ATGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATCATTGGAA CCAAGATCAA AGAAGGAAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATCGAGCGG TCGGCATCAC TCATCTCCCA AGCAGAACAC 
TCTCTGAAGG AGAATGATCA CGCGCGTTTC CTACAGACCG CTAAGAATAT CACTGAGAGA 
GTCTCCATGG CAACAGCATC ATCCCAGGTC CTCATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TCGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACGGCTTC CTATGACACC 
ATAACCGTTC ACTGGACCTC TGATGACGAA TTCAGTGTGG TTTCCTATGA GCTCCAGTAC 
ACCATCTTCA CTGGACAAGC CAATGTTGTG AGTCTGTGCA ACTCGGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCATG GGCTGCAGAG CGGCACCAAG 
TACATCTTCA TTGTCAAGGC CATCAACCAG GCAGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACGA ACAGCCAACC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTAAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGCCACACG 
CCTGAGCGCT TCACCAGCCA AGGCAGCTAT GGCGTAGCTG GAAACGTGTT CATTGATAGT 
GGCCGCCATT ACTGGGAAGT GGTCATAAGC GGAAGCACAT GGTATGCCAT TGGCCTTGCA 
TACAAGTCAG CCCCAAAGCA CGAGTGGATT GGCAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGCCACA ATACCTGGGT GGTAAGACAC AACAGCAAGG AGATCCCCAT CGAGCCAGCC 
CCCCACCTCC GGCGTGTCGG CATTCTGCTA GACTACGATA ATGGCTCCAT CGCTTTCTAT 
GATGCCTTGA ACTCCATCCA CCTCTACACT TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACCTTCA CTGTGTGGAA CAAATGTCTG ACCATCATAA CAGGCCTTCC CATCCCTGAC 
CATCTAGACT GTACAGAGCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001192822.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179751.1 Bos taurus midline 1 (MID1), mRNA.

Price & Availability↑

CloneID	OBa35830
Clone ID Related Accession (Same CDS sequence)	XM_005228472.4 , NM_001192822.1 , XM_005228472.3 , XM_005228477.3
Accession Version	NM_001192822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000030.1. On Jul 17, 2010 this sequence version replaced XM_614425.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.27141.1, SRR5721694.1808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179751.1
CDS	60..2063
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_001192822.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192822.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005228472.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228529.1 Bos taurus midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35830
Clone ID Related Accession (Same CDS sequence)	XM_005228472.4 , NM_001192822.1 , XM_005228472.3 , XM_005228477.3
Accession Version	XM_005228472.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228472.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228529.1
CDS	495..2498
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005228472.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228472.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005228472.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228529.1 Bos taurus midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35830
Clone ID Related Accession (Same CDS sequence)	XM_005228472.4 , NM_001192822.1 , XM_005228472.3 , XM_005228477.3
Accession Version	XM_005228472.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104781.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228529.1
CDS	212..2215
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005228472.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228472.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005228477.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228534.1 Bos taurus midline 1 (MID1), transcript variant X2, mRNA.

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CloneID	OBa35830
Clone ID Related Accession (Same CDS sequence)	XM_005228472.4 , NM_001192822.1 , XM_005228472.3 , XM_005228477.3
Accession Version	XM_005228477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104781.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228534.1
CDS	299..2302
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANSMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005228477.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228477.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT CGAGGACCCT 
CTCCTGCTCC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAG 
AACATCATCG ACAGGTTCCA GAAAGCTTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCATTTGA CGCCAACAGC ATGACCTCGG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCC GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGACG AGTGCCTAAA AGCCACTCAC CCGAACAAGA AGCCCTTCAC AGGCCACCGT 
CTCATTGAGC CCATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA ACACGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAGCTG 
GTCGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACGGAACT GGAGACGCTT 
TTGGCTAAAC TCATTCAAAC ATGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAATGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATCATT 
GGAACCAAGA TCAAAGAAGG AAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGGTCGGCA TCACTCATCT CCCAAGCAGA ACACTCTCTG 
AAGGAGAATG ATCACGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACAG CATCATCCCA GGTCCTCATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTCGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATAACC 
GTTCACTGGA CCTCTGATGA CGAATTCAGT GTGGTTTCCT ATGAGCTCCA GTACACCATC 
TTCACTGGAC AAGCCAATGT TGTGAGTCTG TGCAACTCGG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CATGGGCTGC AGAGCGGCAC CAAGTACATC 
TTCATTGTCA AGGCCATCAA CCAGGCAGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACGAACAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGCCA CACGCCTGAG 
CGCTTCACCA GCCAAGGCAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGC 
CATTACTGGG AAGTGGTCAT AAGCGGAAGC ACATGGTATG CCATTGGCCT TGCATACAAG 
TCAGCCCCAA AGCACGAGTG GATTGGCAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGC 
CACAATACCT GGGTGGTAAG ACACAACAGC AAGGAGATCC CCATCGAGCC AGCCCCCCAC 
CTCCGGCGTG TCGGCATTCT GCTAGACTAC GATAATGGCT CCATCGCTTT CTATGATGCC 
TTGAACTCCA TCCACCTCTA CACTTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACTGTGT GGAACAAATG TCTGACCATC ATAACAGGCC TTCCCATCCC TGACCATCTA 
GACTGTACAG AGCAGCTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Widely spaced alternative promoters, conserved between human and rodent, control expression of the Opitz syndrome gene MID1.
Genomics80(5)499-508(2002 Nov)
Landry JR,Mager DL

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MID1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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