MADD cDNA ORF clone, Bos taurus(cattle)

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222853 XM_024975351.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222853 XM_024975349.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222853 XM_024975352.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222853 XM_024975348.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222854 XM_024975353.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222855 XM_024975354.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222856 XM_024975355.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222857 XM_024975356.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222858 XM_024975358.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222859 XM_024975359.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222860 XM_024975360.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222861 XM_024975362.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222862 XM_024975363.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222863 XM_024975364.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222864 XM_024975365.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222865 XM_024975366.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222866 XM_024975367.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222867 XM_024975368.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222868 XM_024975371.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa92805 XM_015474893.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92805 XM_015474895.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92805 XM_015474897.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92805 XM_015474894.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92805 XM_015474896.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92806 XM_015474898.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92807 XM_015474899.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92808 XM_015474900.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92809 XM_015474901.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92810 XM_015474902.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92811 XM_015474903.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92812 XM_015474904.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92813 XM_015474905.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92814 XM_015474906.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92815 XM_015474908.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92816 XM_015474909.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa92817 XM_015474913.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa26755 NM_001193078.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26757 XM_015474907.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26757 XM_024975357.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26759 XM_015474910.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26760 XM_015474911.2
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26761 XM_015474912.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26762 XM_015474914.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26762 XM_024975370.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26763 XM_015474915.2
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26764 XM_015474916.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26765 XM_024975372.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26765 XM_015474917.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26766 XM_015474918.2
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26767 XM_015474919.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26768 XM_015474920.2
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26769 XM_015474921.1
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa52688 XM_010812836.2
Latest version!
Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa26763 XM_015474915.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26766 XM_015474918.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26760 XM_015474911.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa26768 XM_015474920.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222853
    Clone ID Related Accession (Same CDS sequence) XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
    Accession Version XM_024975352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831120.1
    CDS231..5180
    Translation

    Target ORF information:

    RefSeq Version XM_024975352.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222853
    Clone ID Related Accession (Same CDS sequence) XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
    Accession Version XM_024975349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831117.1
    CDS235..5184
    Translation

    Target ORF information:

    RefSeq Version XM_024975349.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222853
    Clone ID Related Accession (Same CDS sequence) XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
    Accession Version XM_024975348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831116.1
    CDS238..5187
    Translation

    Target ORF information:

    RefSeq Version XM_024975348.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222853
    Clone ID Related Accession (Same CDS sequence) XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
    Accession Version XM_024975351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831119.1
    CDS281..5230
    Translation

    Target ORF information:

    RefSeq Version XM_024975351.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222854
    Clone ID Related Accession (Same CDS sequence) XM_024975353.1
    Accession Version XM_024975353.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC
    TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG
    ACCCCGCCCC GGCCCGTCTC TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831121.1
    CDS238..5184
    Translation

    Target ORF information:

    RefSeq Version XM_024975353.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC
    TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG
    ACCCCGCCCC GGCCCGTCTC TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222855
    Clone ID Related Accession (Same CDS sequence) XM_024975354.1
    Accession Version XM_024975354.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG
    GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA
    GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC
    CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC
    GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA
    CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA
    CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG
    GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC
    ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC
    CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC
    ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG
    TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG
    ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831122.1
    CDS238..5175
    Translation

    Target ORF information:

    RefSeq Version XM_024975354.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG
    GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA
    GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC
    CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC
    GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA
    CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA
    CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG
    GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC
    ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC
    CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC
    ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG
    TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG
    ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222856
    Clone ID Related Accession (Same CDS sequence) XM_024975355.1
    Accession Version XM_024975355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG
    CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA
    TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG
    CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC
    TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG
    AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG
    AAGAGCACGC CCTTCCCCAG TCTGAAAGTA TTTGGGCTAA ATACTCTAAT GGAGATTGTT
    ACTGAAGCCG GCCCCGGGAG TGGTGAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG
    TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC
    AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC
    CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG
    CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG
    GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC
    AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC
    AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG
    CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT
    GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA
    CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC
    TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG
    GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA
    AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT
    CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT
    GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC
    AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA
    GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA
    GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT
    GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG
    ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT
    ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG
    AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG
    GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG
    CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG
    GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA
    GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC
    CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC
    TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC
    GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG
    TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT
    CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC
    ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG
    GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT
    GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG
    ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC
    AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831123.1
    CDS238..5145
    Translation

    Target ORF information:

    RefSeq Version XM_024975355.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG
    CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA
    TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG
    CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC
    TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG
    AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG
    AAGAGCACGC CCTTCCCCAG TCTGAAAGTA TTTGGGCTAA ATACTCTAAT GGAGATTGTT
    ACTGAAGCCG GCCCCGGGAG TGGTGAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG
    TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC
    AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC
    CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG
    CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG
    GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC
    AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC
    AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG
    CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT
    GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA
    CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC
    TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG
    GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA
    AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT
    CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT
    GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC
    AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA
    GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA
    GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT
    GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG
    ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT
    ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG
    AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG
    GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG
    CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG
    GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA
    GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC
    CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC
    TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC
    GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG
    TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT
    CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC
    ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG
    GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT
    GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG
    ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC
    AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222857
    Clone ID Related Accession (Same CDS sequence) XM_024975356.1
    Accession Version XM_024975356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCGAT GGCCCACGAG
    ATCTGCTACT CCGTGTTGTG TCTCTTCTCG TACGTGGCTG CCGTCCGCAG CAGTGAGGAG
    GACCTCAGGA CCCCGCCCCG GCCCGTCTCT AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831124.1
    CDS238..5133
    Translation

    Target ORF information:

    RefSeq Version XM_024975356.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCGAT GGCCCACGAG
    ATCTGCTACT CCGTGTTGTG TCTCTTCTCG TACGTGGCTG CCGTCCGCAG CAGTGAGGAG
    GACCTCAGGA CCCCGCCCCG GCCCGTCTCT AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222858
    Clone ID Related Accession (Same CDS sequence) XM_024975358.1
    Accession Version XM_024975358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC
    TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG
    ACCCCGCCCC GGCCCGTCTC TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831126.1
    CDS238..5124
    Translation

    Target ORF information:

    RefSeq Version XM_024975358.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC
    TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG
    ACCCCGCCCC GGCCCGTCTC TAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222859
    Clone ID Related Accession (Same CDS sequence) XM_024975359.1
    Accession Version XM_024975359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831127.1
    CDS238..5121
    Translation

    Target ORF information:

    RefSeq Version XM_024975359.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222860
    Clone ID Related Accession (Same CDS sequence) XM_024975360.1
    Accession Version XM_024975360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG
    CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA
    TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG
    CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC
    TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG
    AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG
    AAGAGCACGC CCTTCCCCAG TCTGAAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG
    TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC
    AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC
    CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG
    CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG
    GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC
    AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC
    AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG
    CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT
    GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA
    CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC
    TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG
    GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA
    AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT
    CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT
    GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC
    AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA
    GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA
    GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT
    GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG
    ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT
    ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG
    AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG
    GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG
    CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG
    GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA
    GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC
    CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC
    TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC
    GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG
    TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT
    CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC
    ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG
    GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT
    GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG
    ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC
    AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831128.1
    CDS238..5085
    Translation

    Target ORF information:

    RefSeq Version XM_024975360.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG
    CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA
    TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG
    CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC
    TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG
    AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG
    AAGAGCACGC CCTTCCCCAG TCTGAAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG
    TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC
    AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC
    CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG
    CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG
    GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC
    AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC
    AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG
    CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT
    GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA
    CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC
    TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG
    GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA
    AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT
    CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT
    GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC
    AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA
    GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA
    GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT
    GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG
    ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT
    ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG
    AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG
    GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG
    CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG
    GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA
    GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC
    CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC
    TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC
    GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG
    TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT
    CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC
    ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG
    GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT
    GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG
    ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC
    AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222861
    Clone ID Related Accession (Same CDS sequence) XM_024975362.1
    Accession Version XM_024975362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC
    GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG
    GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831130.1
    CDS238..5076
    Translation

    Target ORF information:

    RefSeq Version XM_024975362.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC
    GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG
    GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222862
    Clone ID Related Accession (Same CDS sequence) XM_024975363.1
    Accession Version XM_024975363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC
    TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC
    CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831131.1
    CDS1..4833
    Translation

    Target ORF information:

    RefSeq Version XM_024975363.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC
    TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC
    CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222863
    Clone ID Related Accession (Same CDS sequence) XM_024975364.1
    Accession Version XM_024975364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT
    GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGAATTGTGG
    AGCAAGCACC AGGAAGTGAA AAATCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC
    AAACCCACCT TTGACCTTGG TGAGACCGAC GAGAAAAAGT CCCAGGTCAG CGCAGACAGT
    GGTGTGAGCC TGACATCTGG TTCCCAGAGG ACCGATCCAG ACTCTGTCAT CAGTGTGAGT
    CCCGCTGTGA TGATCCGAAG CTCAAGTCAG GACTCTGAAG TTAGCACCGT GGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831132.1
    CDS238..5061
    Translation

    Target ORF information:

    RefSeq Version XM_024975364.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT
    GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGAATTGTGG
    AGCAAGCACC AGGAAGTGAA AAATCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC
    AAACCCACCT TTGACCTTGG TGAGACCGAC GAGAAAAAGT CCCAGGTCAG CGCAGACAGT
    GGTGTGAGCC TGACATCTGG TTCCCAGAGG ACCGATCCAG ACTCTGTCAT CAGTGTGAGT
    CCCGCTGTGA TGATCCGAAG CTCAAGTCAG GACTCTGAAG TTAGCACCGT GGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222864
    Clone ID Related Accession (Same CDS sequence) XM_024975365.1
    Accession Version XM_024975365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA
    GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT
    CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG
    CCCGTCTCTA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831133.1
    CDS1..4815
    Translation

    Target ORF information:

    RefSeq Version XM_024975365.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA
    GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT
    CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG
    CCCGTCTCTA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222865
    Clone ID Related Accession (Same CDS sequence) XM_024975366.1
    Accession Version XM_024975366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG
    GAAGACGCGT TCTTAGATGC CGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT
    CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG
    GACGATGAAG ACCGTCTGCT GGCCACACTC TTGCACAACC TCATCTCGTA CATGCTGCTG
    ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT
    ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT
    GGACGCGATC TCTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG
    GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC
    GTGGTGCTGC GCAGTAACAT CGGGACGGTG TACGAGCGCT GGTGGTACGA GAAACTCATC
    AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA TGGCTCCGAG
    ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC
    AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ATTGGGTGGC
    GAGTTCCCCG TGCAGGACAT GAAGACTGGC GAGGGGGGCT TGCTGCAGGT CACCCTCGAA
    GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG
    GTGAGCCACA AGTACAAGAC ACCGATGGCC CACGAGATCT GCTACTCCGT GTTGTGTCTC
    TTCTCGTACG TGGCTGCCGT CCGCAGCAGT GAGGAGGACC TCAGGACCCC GCCCCGGCCC
    GTCTCTAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831134.1
    CDS238..5049
    Translation

    Target ORF information:

    RefSeq Version XM_024975366.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC
    AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA
    GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC
    GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC
    CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG
    GAAGACGCGT TCTTAGATGC CGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT
    CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG
    GACGATGAAG ACCGTCTGCT GGCCACACTC TTGCACAACC TCATCTCGTA CATGCTGCTG
    ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT
    ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT
    GGACGCGATC TCTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG
    GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC
    GTGGTGCTGC GCAGTAACAT CGGGACGGTG TACGAGCGCT GGTGGTACGA GAAACTCATC
    AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA TGGCTCCGAG
    ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC
    AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ATTGGGTGGC
    GAGTTCCCCG TGCAGGACAT GAAGACTGGC GAGGGGGGCT TGCTGCAGGT CACCCTCGAA
    GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG
    GTGAGCCACA AGTACAAGAC ACCGATGGCC CACGAGATCT GCTACTCCGT GTTGTGTCTC
    TTCTCGTACG TGGCTGCCGT CCGCAGCAGT GAGGAGGACC TCAGGACCCC GCCCCGGCCC
    GTCTCTAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222866
    Clone ID Related Accession (Same CDS sequence) XM_024975367.1
    Accession Version XM_024975367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GGCTCAAAAG CTGCTGCGGC CCAGCAGCTT GAAGCTGGCA
    AGTGACTCAG ACGCAGAGTC GGACTCTCGA GCGAGCTCGC CCACCTCCAC TGTCTCCAGC
    AACAGCACGG AGGGCTTCGG GGGCATCATG TCTTTGGCCA GCAGCCTGTA TCGGAACCAC
    AGCACCAGCT TCAGCCTTTC GAACCTCACA CTGCCCACCA AAGGAGCCCG AGAGAAGAGC
    ACGCCCTTCC CCAGTCTGAA AGTATTTGGG CTAAATACTC TAATGGAGAT TGTTACTGAA
    GCCGGCCCCG GGAGTGGTGA AGGGCACAGG CGGGCGTTGG TGGACCAGAA GTCGTCCGTC
    ATTAAACACA GCCCGACCGT GAAGAGAGAG CCCCCGTCAC CCCAGGGCCG AGCCAGCAAC
    TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGC
    GTCGGCTGGC TCAACATGAA GAAGGTGCGG CGGCTGCTGG AGAGCGAGCA GCTGCGCGTC
    TTCGTCCTGA GCAAGCTGAA CCGCACGGTG CAGTCCGAGG ACGAGGCCCG GCAGGATGTC
    ATCCCCGACG TGGAGATCAG CCGGAAGGTG TACAAGGGCA TGTTGGATCT GCTCAAGTGC
    ACAGTCCTCA GTCTGGAGCA GTCCTTTGCC CACGCTGGCC TGGGTGGCAT GGCCAGCATC
    TTCGGGCTTC TGGAGATCGC CCAGACCCAC TACTACAGTA AAGAACCAGA CAAGCGGAAG
    AGAAGTCCCA CAGAGAGCAT CAGTACACCA GTCGGCAAGG ATCCTGGCCT GGCTGGGCGG
    GGGGACCCAA AGGCCATGGC ACAGCTGAGG GTCCCCCAGC TGGGACCTCT GGCACCGAGT
    GCCCCAGGAA AGAGTCCCAA AGAACTGGAC ACCAGAAGTC TAAAGGAAGA GAACTTTGTG
    GCATCTATCG AATTGTGGAG CAAGCACCAG GAAGTGAAAA ATCAAAAAGC TTTGGAAAAA
    CAGAGGCCTG AAGTAATCAA ACCCACCTTT GACCTTGGTG AGACCGACGA GAAAAAGTCC
    CAGGTCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC CGATCCAGAC
    TCTGTCATCA GTGTGAGTCC CGCTGTGATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGCAACAG CTCCGGAGAG ACCCTGGGAG CAGACAGCGA CCTCAGCAGC
    AGTGCGGGTG ATGGCCCAGG CGGCGAGGGC AGCGCCTACT TGGCAGGCTC TCGAGGCACC
    TTGTCTGACA GCGAGATCGA GACCAACTCT GCCACCAGTG CCATCTTTGG TAAAGCCCAT
    AGCTTGAAGC CAAGTGTGAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTT TTCTGAAGAC
    GTCAGCCAGC GAGTCTACCT CTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGACGCTGC TATGGAGACC TTTTCTATAA GCAAAGAGCG TTCTACTTTA
    TGGGACCAAA TGCAGTTCTG GGAAGACGCG TTCTTAGATG CCGTGATGTT GGAGAGAGAA
    GGAATGGGCA TGGACCAGGG TCCCCAGGAA ATGATAGACA GGTACCTGTC CCTGGGAGAA
    CACGACCGGA AGCGCCTGGA GGACGATGAA GACCGTCTGC TGGCCACACT CTTGCACAAC
    CTCATCTCGT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTGATGG GAAAGTCCCA TATTGGGCTT GTGTACAGTC AGCAAATCAA TGAAGTCCTT
    GACCAGCTGG CCAACCTGAA TGGACGCGAT CTCTCCATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAGAAGC AGACATTCGT GGTACATGCA GGGACAGACA CGAATGGAGA TATCTTTTTC
    ATGGAGGTGT GTGACGACTG CGTGGTGCTG CGCAGTAACA TCGGGACGGT GTACGAGCGC
    TGGTGGTACG AGAAACTCAT CAACATGACC TACTGCCCCA AGACCAAGGT GCTGTGCTTG
    TGGCGCAGAA ATGGCTCCGA GACCCAGCTC AACAAGTTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GCGTGAAGGA CAGCATGGAG CGCGCCGCCG CCCGCCAGCA GAGCATCAAG
    CCGGGGCCGG AATTGGGTGG CGAGTTCCCC GTGCAGGACA TGAAGACTGG CGAGGGGGGC
    TTGCTGCAGG TCACCCTCGA AGGGATCAAC CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCGATGGCC
    CACGAGATCT GCTACTCCGT GTTGTGTCTC TTCTCGTACG TGGCTGCCGT CCGCAGCAGT
    GAGGAGGACC TCAGGACCCC GCCCCGGCCC GTCTCTAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831135.1
    CDS238..5019
    Translation

    Target ORF information:

    RefSeq Version XM_024975367.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC
    GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT
    CAGACAGAAA CCGGAGAGGG GGCTCAAAAG CTGCTGCGGC CCAGCAGCTT GAAGCTGGCA
    AGTGACTCAG ACGCAGAGTC GGACTCTCGA GCGAGCTCGC CCACCTCCAC TGTCTCCAGC
    AACAGCACGG AGGGCTTCGG GGGCATCATG TCTTTGGCCA GCAGCCTGTA TCGGAACCAC
    AGCACCAGCT TCAGCCTTTC GAACCTCACA CTGCCCACCA AAGGAGCCCG AGAGAAGAGC
    ACGCCCTTCC CCAGTCTGAA AGTATTTGGG CTAAATACTC TAATGGAGAT TGTTACTGAA
    GCCGGCCCCG GGAGTGGTGA AGGGCACAGG CGGGCGTTGG TGGACCAGAA GTCGTCCGTC
    ATTAAACACA GCCCGACCGT GAAGAGAGAG CCCCCGTCAC CCCAGGGCCG AGCCAGCAAC
    TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGC
    GTCGGCTGGC TCAACATGAA GAAGGTGCGG CGGCTGCTGG AGAGCGAGCA GCTGCGCGTC
    TTCGTCCTGA GCAAGCTGAA CCGCACGGTG CAGTCCGAGG ACGAGGCCCG GCAGGATGTC
    ATCCCCGACG TGGAGATCAG CCGGAAGGTG TACAAGGGCA TGTTGGATCT GCTCAAGTGC
    ACAGTCCTCA GTCTGGAGCA GTCCTTTGCC CACGCTGGCC TGGGTGGCAT GGCCAGCATC
    TTCGGGCTTC TGGAGATCGC CCAGACCCAC TACTACAGTA AAGAACCAGA CAAGCGGAAG
    AGAAGTCCCA CAGAGAGCAT CAGTACACCA GTCGGCAAGG ATCCTGGCCT GGCTGGGCGG
    GGGGACCCAA AGGCCATGGC ACAGCTGAGG GTCCCCCAGC TGGGACCTCT GGCACCGAGT
    GCCCCAGGAA AGAGTCCCAA AGAACTGGAC ACCAGAAGTC TAAAGGAAGA GAACTTTGTG
    GCATCTATCG AATTGTGGAG CAAGCACCAG GAAGTGAAAA ATCAAAAAGC TTTGGAAAAA
    CAGAGGCCTG AAGTAATCAA ACCCACCTTT GACCTTGGTG AGACCGACGA GAAAAAGTCC
    CAGGTCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC CGATCCAGAC
    TCTGTCATCA GTGTGAGTCC CGCTGTGATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGCAACAG CTCCGGAGAG ACCCTGGGAG CAGACAGCGA CCTCAGCAGC
    AGTGCGGGTG ATGGCCCAGG CGGCGAGGGC AGCGCCTACT TGGCAGGCTC TCGAGGCACC
    TTGTCTGACA GCGAGATCGA GACCAACTCT GCCACCAGTG CCATCTTTGG TAAAGCCCAT
    AGCTTGAAGC CAAGTGTGAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTT TTCTGAAGAC
    GTCAGCCAGC GAGTCTACCT CTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGACGCTGC TATGGAGACC TTTTCTATAA GCAAAGAGCG TTCTACTTTA
    TGGGACCAAA TGCAGTTCTG GGAAGACGCG TTCTTAGATG CCGTGATGTT GGAGAGAGAA
    GGAATGGGCA TGGACCAGGG TCCCCAGGAA ATGATAGACA GGTACCTGTC CCTGGGAGAA
    CACGACCGGA AGCGCCTGGA GGACGATGAA GACCGTCTGC TGGCCACACT CTTGCACAAC
    CTCATCTCGT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTGATGG GAAAGTCCCA TATTGGGCTT GTGTACAGTC AGCAAATCAA TGAAGTCCTT
    GACCAGCTGG CCAACCTGAA TGGACGCGAT CTCTCCATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAGAAGC AGACATTCGT GGTACATGCA GGGACAGACA CGAATGGAGA TATCTTTTTC
    ATGGAGGTGT GTGACGACTG CGTGGTGCTG CGCAGTAACA TCGGGACGGT GTACGAGCGC
    TGGTGGTACG AGAAACTCAT CAACATGACC TACTGCCCCA AGACCAAGGT GCTGTGCTTG
    TGGCGCAGAA ATGGCTCCGA GACCCAGCTC AACAAGTTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GCGTGAAGGA CAGCATGGAG CGCGCCGCCG CCCGCCAGCA GAGCATCAAG
    CCGGGGCCGG AATTGGGTGG CGAGTTCCCC GTGCAGGACA TGAAGACTGG CGAGGGGGGC
    TTGCTGCAGG TCACCCTCGA AGGGATCAAC CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCGATGGCC
    CACGAGATCT GCTACTCCGT GTTGTGTCTC TTCTCGTACG TGGCTGCCGT CCGCAGCAGT
    GAGGAGGACC TCAGGACCCC GCCCCGGCCC GTCTCTAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222867
    Clone ID Related Accession (Same CDS sequence) XM_024975368.1
    Accession Version XM_024975368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC
    TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC
    CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831136.1
    CDS1..4773
    Translation

    Target ORF information:

    RefSeq Version XM_024975368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC
    TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC
    CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222868
    Clone ID Related Accession (Same CDS sequence) XM_024975371.1
    Accession Version XM_024975371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG
    GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA
    GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC
    CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC
    GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA
    CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA
    CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG
    GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC
    ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC
    CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC
    ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG
    TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG
    ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831139.1
    CDS238..4968
    Translation

    Target ORF information:

    RefSeq Version XM_024975371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG
    GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA
    GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC
    CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC
    GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA
    CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA
    CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG
    GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC
    ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC
    CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC
    ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG
    TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG
    ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92805
    Clone ID Related Accession (Same CDS sequence) XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
    Accession Version XM_015474894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330380.1
    CDS358..5325
    Translation

    Target ORF information:

    RefSeq Version XM_015474894.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92805
    Clone ID Related Accession (Same CDS sequence) XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
    Accession Version XM_015474897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330383.1
    CDS713..5680
    Translation

    Target ORF information:

    RefSeq Version XM_015474897.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92805
    Clone ID Related Accession (Same CDS sequence) XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
    Accession Version XM_015474893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330379.1
    CDS274..5241
    Translation

    Target ORF information:

    RefSeq Version XM_015474893.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92805
    Clone ID Related Accession (Same CDS sequence) XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
    Accession Version XM_015474895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330381.1
    CDS512..5479
    Translation

    Target ORF information:

    RefSeq Version XM_015474895.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92805
    Clone ID Related Accession (Same CDS sequence) XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
    Accession Version XM_015474896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330382.1
    CDS282..5249
    Translation

    Target ORF information:

    RefSeq Version XM_015474896.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92806
    Clone ID Related Accession (Same CDS sequence) XM_015474898.1
    Accession Version XM_015474898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC
    GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG
    AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330384.1
    CDS274..5238
    Translation

    Target ORF information:

    RefSeq Version XM_015474898.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC
    GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG
    AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92807
    Clone ID Related Accession (Same CDS sequence) XM_015474899.1
    Accession Version XM_015474899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC
    GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG
    GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330385.1
    CDS273..5231
    Translation

    Target ORF information:

    RefSeq Version XM_015474899.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC
    GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG
    GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92808
    Clone ID Related Accession (Same CDS sequence) XM_015474900.1
    Accession Version XM_015474900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT
    AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT
    CCAGAGGAGG GACTAGAGAC AGAGAAGAAG CGCCTCTGCA GAGAGGTCAG GAGGAGCAGG
    AGCGTAGGGA GGATGAGAGA AGGGAGGCAG GAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330386.1
    CDS274..5232
    Translation

    Target ORF information:

    RefSeq Version XM_015474900.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT
    AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT
    CCAGAGGAGG GACTAGAGAC AGAGAAGAAG CGCCTCTGCA GAGAGGTCAG GAGGAGCAGG
    AGCGTAGGGA GGATGAGAGA AGGGAGGCAG GAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92809
    Clone ID Related Accession (Same CDS sequence) XM_015474901.1
    Accession Version XM_015474901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT
    TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA
    GAGGAGGGAC TAGAGACAGA GAAGAAGCGC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC
    GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330387.1
    CDS274..5229
    Translation

    Target ORF information:

    RefSeq Version XM_015474901.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT
    TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA
    GAGGAGGGAC TAGAGACAGA GAAGAAGCGC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC
    GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92810
    Clone ID Related Accession (Same CDS sequence) XM_015474902.1
    Accession Version XM_015474902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG
    GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG
    GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG
    TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC
    ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC
    AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC
    TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC
    CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA
    GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG
    GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGGC ACAGGCGGGC GTTGGTGGAC
    CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG
    GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG
    CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC
    GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG
    GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG
    GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT
    GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA
    CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT
    GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA
    CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG
    GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA
    AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC
    GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG
    AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT
    CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC
    AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA
    GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC
    TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT
    CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC
    AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA
    GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG
    ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC
    CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC
    ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC
    CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA
    ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC
    AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT
    GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG
    ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC
    AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT
    AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC
    CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG
    ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC
    AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA
    GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA
    CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC
    CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330388.1
    CDS274..5199
    Translation

    Target ORF information:

    RefSeq Version XM_015474902.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG
    GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG
    GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG
    TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC
    ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC
    AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC
    TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC
    CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA
    GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG
    GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGGC ACAGGCGGGC GTTGGTGGAC
    CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG
    GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG
    CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC
    GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG
    GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG
    GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT
    GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA
    CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT
    GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA
    CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG
    GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA
    AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC
    GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG
    AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT
    CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC
    AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA
    GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC
    TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT
    CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC
    AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA
    GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG
    ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC
    CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC
    ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC
    CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA
    ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC
    AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT
    GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG
    ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC
    AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT
    AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC
    CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG
    ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC
    AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA
    GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA
    CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC
    CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92811
    Clone ID Related Accession (Same CDS sequence) XM_015474903.1
    Accession Version XM_015474903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG
    TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA
    GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA
    GTTAGCACCG TGGTGAGCAA CAGCTCCGGA GAGACCCTGG GAGCAGACAG CGACCTCAGC
    AGCAGTGCGG GTGATGGCCC AGGCGGCGAG GGCAGCGCCT ACTTGGCAGG CTCTCGAGGC
    ACCTTGTCTG ACAGCGAGAT CGAGACCAAC TCTGCCACCA GTGCCATCTT TGGTAAAGCC
    CATAGCTTGA AGCCAAGTGT GAAGGAGAAG CTGGTGGGCA GCCCAGTTCG CTTTTCTGAA
    GACGTCAGCC AGCGAGTCTA CCTCTACGAG GGACTCCTAG GAAGGGACAA AGGATCGATG
    TGGGACCAGT TAGAGGACGC TGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT
    TTATGGGACC AAATGCAGTT CTGGGAAGAC GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA
    GAAGGAATGG GCATGGACCA GGGTCCCCAG GAAATGATAG ACAGGTACCT GTCCCTGGGA
    GAACACGACC GGAAGCGCCT GGAGGACGAT GAAGACCGTC TGCTGGCCAC ACTCTTGCAC
    AACCTCATCT CGTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG GAAGAAGGTG
    AGGCGCCTGA TGGGAAAGTC CCATATTGGG CTTGTGTACA GTCAGCAAAT CAATGAAGTC
    CTTGACCAGC TGGCCAACCT GAATGGACGC GATCTCTCCA TCCGGTCCAG TGGCAGCCGG
    CACATGAAGA AGCAGACATT CGTGGTACAT GCAGGGACAG ACACGAATGG AGATATCTTT
    TTCATGGAGG TGTGTGACGA CTGCGTGGTG CTGCGCAGTA ACATCGGGAC GGTGTACGAG
    CGCTGGTGGT ACGAGAAACT CATCAACATG ACCTACTGCC CCAAGACCAA GGTGCTGTGC
    TTGTGGCGCA GAAATGGCTC CGAGACCCAG CTCAACAAGT TCTATACTAA GAAGTGTCGG
    GAGCTGTACT ACTGCGTGAA GGACAGCATG GAGCGCGCCG CCGCCCGCCA GCAGAGCATC
    AAGCCGGGGC CGGAATTGGG TGGCGAGTTC CCCGTGCAGG ACATGAAGAC TGGCGAGGGG
    GGCTTGCTGC AGGTCACCCT CGAAGGGATC AACCTCAAGT TCATGCACAA CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTCCGAGG CGTCTTTGTC
    CTGGAGGAAT TTGGTAATTA CACTATTTTG CTCTTAGGTC TGGACTCACA TGGCAGTAAC
    TCAAACCTCG GAGCTCCAGA GGAGGGACTA GAGACAGAGA AGAAGCGCCT CTGCAGAGAG
    GTCAGGAGGA GCAGGAGCGT AGGGAGGATG AGAGAAGGGA GGCAGGAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330389.1
    CDS273..5186
    Translation

    Target ORF information:

    RefSeq Version XM_015474903.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG
    TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA
    GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA
    GTTAGCACCG TGGTGAGCAA CAGCTCCGGA GAGACCCTGG GAGCAGACAG CGACCTCAGC
    AGCAGTGCGG GTGATGGCCC AGGCGGCGAG GGCAGCGCCT ACTTGGCAGG CTCTCGAGGC
    ACCTTGTCTG ACAGCGAGAT CGAGACCAAC TCTGCCACCA GTGCCATCTT TGGTAAAGCC
    CATAGCTTGA AGCCAAGTGT GAAGGAGAAG CTGGTGGGCA GCCCAGTTCG CTTTTCTGAA
    GACGTCAGCC AGCGAGTCTA CCTCTACGAG GGACTCCTAG GAAGGGACAA AGGATCGATG
    TGGGACCAGT TAGAGGACGC TGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT
    TTATGGGACC AAATGCAGTT CTGGGAAGAC GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA
    GAAGGAATGG GCATGGACCA GGGTCCCCAG GAAATGATAG ACAGGTACCT GTCCCTGGGA
    GAACACGACC GGAAGCGCCT GGAGGACGAT GAAGACCGTC TGCTGGCCAC ACTCTTGCAC
    AACCTCATCT CGTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG GAAGAAGGTG
    AGGCGCCTGA TGGGAAAGTC CCATATTGGG CTTGTGTACA GTCAGCAAAT CAATGAAGTC
    CTTGACCAGC TGGCCAACCT GAATGGACGC GATCTCTCCA TCCGGTCCAG TGGCAGCCGG
    CACATGAAGA AGCAGACATT CGTGGTACAT GCAGGGACAG ACACGAATGG AGATATCTTT
    TTCATGGAGG TGTGTGACGA CTGCGTGGTG CTGCGCAGTA ACATCGGGAC GGTGTACGAG
    CGCTGGTGGT ACGAGAAACT CATCAACATG ACCTACTGCC CCAAGACCAA GGTGCTGTGC
    TTGTGGCGCA GAAATGGCTC CGAGACCCAG CTCAACAAGT TCTATACTAA GAAGTGTCGG
    GAGCTGTACT ACTGCGTGAA GGACAGCATG GAGCGCGCCG CCGCCCGCCA GCAGAGCATC
    AAGCCGGGGC CGGAATTGGG TGGCGAGTTC CCCGTGCAGG ACATGAAGAC TGGCGAGGGG
    GGCTTGCTGC AGGTCACCCT CGAAGGGATC AACCTCAAGT TCATGCACAA CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTCCGAGG CGTCTTTGTC
    CTGGAGGAAT TTGGTAATTA CACTATTTTG CTCTTAGGTC TGGACTCACA TGGCAGTAAC
    TCAAACCTCG GAGCTCCAGA GGAGGGACTA GAGACAGAGA AGAAGCGCCT CTGCAGAGAG
    GTCAGGAGGA GCAGGAGCGT AGGGAGGATG AGAGAAGGGA GGCAGGAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92812
    Clone ID Related Accession (Same CDS sequence) XM_015474904.1
    Accession Version XM_015474904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330390.1
    CDS274..5181
    Translation

    Target ORF information:

    RefSeq Version XM_015474904.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG
    AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92813
    Clone ID Related Accession (Same CDS sequence) XM_015474905.1
    Accession Version XM_015474905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC
    GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG
    AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330391.1
    CDS274..5178
    Translation

    Target ORF information:

    RefSeq Version XM_015474905.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC
    CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG
    GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC
    CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC
    TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG
    CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG
    AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG
    GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG
    TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC
    AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG
    CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG
    CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC
    GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG
    AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92814
    Clone ID Related Accession (Same CDS sequence) XM_015474906.1
    Accession Version XM_015474906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG
    TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA
    GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA
    GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC
    AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330392.1
    CDS273..5174
    Translation

    Target ORF information:

    RefSeq Version XM_015474906.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG
    TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA
    GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA
    GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC
    AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92815
    Clone ID Related Accession (Same CDS sequence) XM_015474908.1
    Accession Version XM_015474908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG
    GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG
    GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG
    TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC
    ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC
    AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC
    TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC
    CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA
    GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGGGC ACAGGCGGGC GTTGGTGGAC
    CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG
    GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG
    CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC
    GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG
    GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG
    GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT
    GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA
    CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT
    GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA
    CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG
    GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA
    AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC
    GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG
    AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT
    CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC
    AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA
    GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC
    TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT
    CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC
    AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA
    GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG
    ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC
    CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC
    ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC
    CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA
    ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC
    AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT
    GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG
    ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC
    AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT
    AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC
    CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG
    ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC
    AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA
    GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA
    CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC
    CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330394.1
    CDS274..5139
    Translation

    Target ORF information:

    RefSeq Version XM_015474908.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG
    GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG
    GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG
    TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC
    ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC
    AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC
    TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC
    CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA
    GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGGGC ACAGGCGGGC GTTGGTGGAC
    CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG
    GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG
    CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC
    GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG
    GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG
    GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT
    GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA
    CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT
    GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA
    CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG
    GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA
    AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC
    GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG
    AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT
    CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC
    AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA
    GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC
    TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT
    CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC
    AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA
    GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG
    ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC
    CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC
    ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC
    CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA
    ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC
    AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT
    GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG
    ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC
    AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT
    AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC
    CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG
    ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC
    AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA
    GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA
    CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC
    CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92816
    Clone ID Related Accession (Same CDS sequence) XM_015474909.1
    Accession Version XM_015474909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGGCTCAAAA GCTGCTGCGG CCCAGCAGCT TGAAGCTGGC AAGTGACTCA
    GACGCAGAGT CGGACTCTCG AGCGAGCTCG CCCACCTCCA CTGTCTCCAG CAACAGCACG
    GAGGGCTTCG GGGGCATCAT GTCTTTGGCC AGCAGCCTGT ATCGGAACCA CAGCACCAGC
    TTCAGCCTTT CGAACCTCAC ACTGCCCACC AAAGGAGCCC GAGAGAAGAG CACGCCCTTC
    CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC
    GGGAGTGGTG AAGGGCACAG GCGGGCGTTG GTGGACCAGA AGTCGTCCGT CATTAAACAC
    AGCCCGACCG TGAAGAGAGA GCCCCCGTCA CCCCAGGGCC GAGCCAGCAA CTCTAGTGAG
    AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG CGTCGGCTGG
    CTCAACATGA AGAAGGTGCG GCGGCTGCTG GAGAGCGAGC AGCTGCGCGT CTTCGTCCTG
    AGCAAGCTGA ACCGCACGGT GCAGTCCGAG GACGAGGCCC GGCAGGATGT CATCCCCGAC
    GTGGAGATCA GCCGGAAGGT GTACAAGGGC ATGTTGGATC TGCTCAAGTG CACAGTCCTC
    AGTCTGGAGC AGTCCTTTGC CCACGCTGGC CTGGGTGGCA TGGCCAGCAT CTTCGGGCTT
    CTGGAGATCG CCCAGACCCA CTACTACAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCC
    ACAGAGAGCA TCAGTACACC AGTCGGCAAG GATCCTGGCC TGGCTGGGCG GGGGGACCCA
    AAGGCCATGG CACAGCTGAG GGTCCCCCAG CTGGGACCTC TGGCACCGAG TGCCCCAGGA
    AAGAGTCCCA AAGAACTGGA CACCAGAAGT CTAAAGGAAG AGAACTTTGT GGCATCTATC
    GAATTGTGGA GCAAGCACCA GGAAGTGAAA AATCAAAAAG CTTTGGAAAA ACAGAGGCCT
    GAAGTAATCA AACCCACCTT TGACCTTGGT GAGACCGACG AGAAAAAGTC CCAGGTCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CCGATCCAGA CTCTGTCATC
    AGTGTGAGTC CCGCTGTGAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA
    GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC
    AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330395.1
    CDS274..5115
    Translation

    Target ORF information:

    RefSeq Version XM_015474909.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGGCTCAAAA GCTGCTGCGG CCCAGCAGCT TGAAGCTGGC AAGTGACTCA
    GACGCAGAGT CGGACTCTCG AGCGAGCTCG CCCACCTCCA CTGTCTCCAG CAACAGCACG
    GAGGGCTTCG GGGGCATCAT GTCTTTGGCC AGCAGCCTGT ATCGGAACCA CAGCACCAGC
    TTCAGCCTTT CGAACCTCAC ACTGCCCACC AAAGGAGCCC GAGAGAAGAG CACGCCCTTC
    CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC
    GGGAGTGGTG AAGGGCACAG GCGGGCGTTG GTGGACCAGA AGTCGTCCGT CATTAAACAC
    AGCCCGACCG TGAAGAGAGA GCCCCCGTCA CCCCAGGGCC GAGCCAGCAA CTCTAGTGAG
    AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG CGTCGGCTGG
    CTCAACATGA AGAAGGTGCG GCGGCTGCTG GAGAGCGAGC AGCTGCGCGT CTTCGTCCTG
    AGCAAGCTGA ACCGCACGGT GCAGTCCGAG GACGAGGCCC GGCAGGATGT CATCCCCGAC
    GTGGAGATCA GCCGGAAGGT GTACAAGGGC ATGTTGGATC TGCTCAAGTG CACAGTCCTC
    AGTCTGGAGC AGTCCTTTGC CCACGCTGGC CTGGGTGGCA TGGCCAGCAT CTTCGGGCTT
    CTGGAGATCG CCCAGACCCA CTACTACAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCC
    ACAGAGAGCA TCAGTACACC AGTCGGCAAG GATCCTGGCC TGGCTGGGCG GGGGGACCCA
    AAGGCCATGG CACAGCTGAG GGTCCCCCAG CTGGGACCTC TGGCACCGAG TGCCCCAGGA
    AAGAGTCCCA AAGAACTGGA CACCAGAAGT CTAAAGGAAG AGAACTTTGT GGCATCTATC
    GAATTGTGGA GCAAGCACCA GGAAGTGAAA AATCAAAAAG CTTTGGAAAA ACAGAGGCCT
    GAAGTAATCA AACCCACCTT TGACCTTGGT GAGACCGACG AGAAAAAGTC CCAGGTCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CCGATCCAGA CTCTGTCATC
    AGTGTGAGTC CCGCTGTGAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA
    GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC
    AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92817
    Clone ID Related Accession (Same CDS sequence) XM_015474913.1
    Accession Version XM_015474913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGTT CCTGAAATTA
    AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330399.1
    CDS273..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015474913.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGTT CCTGAAATTA
    AAGAAGTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26755
    Clone ID Related Accession (Same CDS sequence) NM_001193078.1
    Accession Version NM_001193078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On Jul 17, 2010 this sequence version replaced XM_867337.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG
    TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG
    CCCCGGCCCG TCTCTAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180007.1
    CDS1..4941
    Translation

    Target ORF information:

    RefSeq Version NM_001193078.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG
    TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG
    CCCCGGCCCG TCTCTAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26757
    Clone ID Related Accession (Same CDS sequence) XM_015474907.1 , XM_024975357.1
    Accession Version XM_015474907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330393.1
    CDS1..4890
    Translation

    Target ORF information:

    RefSeq Version XM_015474907.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26757
    Clone ID Related Accession (Same CDS sequence) XM_015474907.1 , XM_024975357.1
    Accession Version XM_024975357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831125.1
    CDS238..5127
    Translation

    Target ORF information:

    RefSeq Version XM_024975357.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC
    TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC
    AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26759
    Clone ID Related Accession (Same CDS sequence) XM_015474910.1
    Accession Version XM_015474910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330396.1
    CDS1..4824
    Translation

    Target ORF information:

    RefSeq Version XM_015474910.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26760
    Clone ID Related Accession (Same CDS sequence) XM_015474911.2 , XM_015474911.1
    Accession Version XM_015474911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330397.1
    CDS1..4764
    Translation

    Target ORF information:

    RefSeq Version XM_015474911.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26760
    Clone ID Related Accession (Same CDS sequence) XM_015474911.2 , XM_015474911.1
    Accession Version XM_015474911.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330397.1
    CDS1..4764
    Translation

    Target ORF information:

    RefSeq Version XM_015474911.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG
    GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT
    GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG
    AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC
    CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA
    ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC
    ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG
    AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG
    GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG
    CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG
    TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC
    GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA
    AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG
    GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG
    GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC
    GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC
    CCGCCCCGGC CCGTCTCTAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26761
    Clone ID Related Accession (Same CDS sequence) XM_015474912.1
    Accession Version XM_015474912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG
    TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG
    CCCCGGCCCG TCTCTAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330398.1
    CDS1..4761
    Translation

    Target ORF information:

    RefSeq Version XM_015474912.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG
    CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC
    ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT
    GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC
    AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG
    CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG
    CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG
    TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG
    CCCCGGCCCG TCTCTAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26762
    Clone ID Related Accession (Same CDS sequence) XM_024975370.1 , XM_015474914.1
    Accession Version XM_024975370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831138.1
    CDS1..4743
    Translation

    Target ORF information:

    RefSeq Version XM_024975370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26762
    Clone ID Related Accession (Same CDS sequence) XM_024975370.1 , XM_015474914.1
    Accession Version XM_015474914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330400.1
    CDS1..4743
    Translation

    Target ORF information:

    RefSeq Version XM_015474914.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26763
    Clone ID Related Accession (Same CDS sequence) XM_015474915.1 , XM_015474915.2
    Accession Version XM_015474915.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330401.1
    CDS1..4740
    Translation

    Target ORF information:

    RefSeq Version XM_015474915.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26763
    Clone ID Related Accession (Same CDS sequence) XM_015474915.1 , XM_015474915.2
    Accession Version XM_015474915.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330401.1
    CDS1..4740
    Translation

    Target ORF information:

    RefSeq Version XM_015474915.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474915.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG
    AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC
    GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26764
    Clone ID Related Accession (Same CDS sequence) XM_015474916.1
    Accession Version XM_015474916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT
    GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330402.1
    CDS1..4698
    Translation

    Target ORF information:

    RefSeq Version XM_015474916.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT
    GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26765
    Clone ID Related Accession (Same CDS sequence) XM_024975372.1 , XM_015474917.1
    Accession Version XM_024975372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831140.1
    CDS1..4683
    Translation

    Target ORF information:

    RefSeq Version XM_024975372.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26765
    Clone ID Related Accession (Same CDS sequence) XM_024975372.1 , XM_015474917.1
    Accession Version XM_015474917.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330403.1
    CDS1..4683
    Translation

    Target ORF information:

    RefSeq Version XM_015474917.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC
    CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT
    GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC
    GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG
    GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC
    AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC
    AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC
    CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC
    CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC
    AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC
    AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG
    GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT
    CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG
    GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA
    GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG
    GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG
    AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA
    TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG
    GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG
    CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG
    GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG
    CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC
    AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG
    ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT
    GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG
    AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT
    GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC
    GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA
    TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC
    ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26766
    Clone ID Related Accession (Same CDS sequence) XM_015474918.1 , XM_015474918.2
    Accession Version XM_015474918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330404.1
    CDS1..4638
    Translation

    Target ORF information:

    RefSeq Version XM_015474918.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26766
    Clone ID Related Accession (Same CDS sequence) XM_015474918.1 , XM_015474918.2
    Accession Version XM_015474918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330404.1
    CDS1..4638
    Translation

    Target ORF information:

    RefSeq Version XM_015474918.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG
    TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC
    CGGCCCGTCT CTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26767
    Clone ID Related Accession (Same CDS sequence) XM_015474919.1
    Accession Version XM_015474919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA
    GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT
    CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG
    CCCGTCTCTA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330405.1
    CDS1..4635
    Translation

    Target ORF information:

    RefSeq Version XM_015474919.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC
    AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC
    CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG
    ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT
    GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC
    TACCTCTACG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC
    TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG
    GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG
    GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG
    AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC
    GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC
    TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC
    ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC
    GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG
    GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT
    GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC
    GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA
    GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT
    CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG
    CCCGTCTCTA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26768
    Clone ID Related Accession (Same CDS sequence) XM_015474920.1 , XM_015474920.2
    Accession Version XM_015474920.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC
    GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG
    GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT
    GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG
    GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG
    CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC
    GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT
    GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG
    CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG
    ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG
    CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG
    GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC
    CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC
    AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC
    CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG
    TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330406.1
    CDS1..4626
    Translation

    Target ORF information:

    RefSeq Version XM_015474920.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC
    GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG
    GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT
    GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG
    GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG
    CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC
    GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT
    GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG
    CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG
    ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG
    CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG
    GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC
    CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC
    AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC
    CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG
    TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26768
    Clone ID Related Accession (Same CDS sequence) XM_015474920.1 , XM_015474920.2
    Accession Version XM_015474920.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC
    GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG
    GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT
    GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG
    GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG
    CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC
    GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT
    GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG
    CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG
    ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG
    CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG
    GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC
    CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC
    AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC
    CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG
    TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330406.1
    CDS1..4626
    Translation

    Target ORF information:

    RefSeq Version XM_015474920.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474920.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC
    GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC
    GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC
    AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG
    AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC
    GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC
    GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG
    GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT
    GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG
    GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG
    CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC
    GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT
    GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG
    CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG
    ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG
    CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG
    GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC
    CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC
    AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC
    CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG
    TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26769
    Clone ID Related Accession (Same CDS sequence) XM_015474921.1
    Accession Version XM_015474921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330407.1
    CDS1..4440
    Translation

    Target ORF information:

    RefSeq Version XM_015474921.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA
    CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG
    CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT
    GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC
    AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG
    GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG
    AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC
    TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC
    AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA
    CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC
    AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC
    TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG
    ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG
    GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC
    AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA
    CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC
    ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG
    GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG
    TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC
    GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT
    TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG
    AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC
    GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG
    TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG
    AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG
    AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC
    AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG
    CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC
    GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC
    ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG
    GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC
    AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA
    GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA
    GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT
    GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG
    AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT
    GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC
    GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA
    AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA
    GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG
    TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC
    CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC
    CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC
    CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA
    TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC
    GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA
    CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC
    TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG
    AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG
    GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC
    CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52688
    Clone ID Related Accession (Same CDS sequence) XM_010812836.2
    Accession Version XM_010812836.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGTTCCTG
    AAATTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811138.1
    CDS274..5211
    Translation

    Target ORF information:

    RefSeq Version XM_010812836.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812836.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
    AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA
    GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC
    TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC
    CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC
    TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA
    GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA
    TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA
    CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC
    AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG
    GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG
    GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG
    CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT
    GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG
    ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC
    CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC
    AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG
    GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG
    GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC
    TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC
    AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA
    TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC
    AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC
    CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC
    TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC
    AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC
    CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT
    TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC
    CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC
    GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT
    GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA
    ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC
    CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG
    GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG
    AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC
    CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC
    AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG
    TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA
    ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT
    GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG
    CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT
    GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC
    AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC
    ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG
    CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC
    GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT
    AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT
    AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC
    GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC
    GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC
    CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA
    GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC
    GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA
    AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG
    GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC
    CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA
    TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG
    AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG
    GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT
    GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC
    ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT
    GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG
    TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC
    TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC
    AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC
    CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG
    GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA
    ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC
    GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC
    ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC
    CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG
    AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG
    GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG
    TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG
    CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG
    GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG
    CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG
    GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC
    CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGTTCCTG
    AAATTAAAGA AGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.