MADD cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADD
Entrez Gene ID	534306
Full Name	MAP kinase activating death domain
General protein information	Preferred Names MAP kinase activating death domain Names MAP kinase-activating death domain protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015474894.1	XP_015330380.1	MAP kinase-activating death domain protein isoform X1
XM_015474898.1	XP_015330384.1	MAP kinase-activating death domain protein isoform X2
XM_015474907.1	XP_015330393.1	MAP kinase-activating death domain protein isoform X12
XM_015474909.1	XP_015330395.1	MAP kinase-activating death domain protein isoform X14
XM_015474915.1	XP_015330401.1	MAP kinase-activating death domain protein isoform X20
XM_024975357.1	XP_024831125.1	MAP kinase-activating death domain protein isoform X6
XM_024975364.1	XP_024831132.1	MAP kinase-activating death domain protein isoform X13
XM_015474915.2	XP_015330401.1	MAP kinase-activating death domain protein isoform X21
XM_015474919.1	XP_015330405.1	MAP kinase-activating death domain protein isoform X26
XM_015474897.1	XP_015330383.1	MAP kinase-activating death domain protein isoform X1
XM_015474899.1	XP_015330385.1	MAP kinase-activating death domain protein isoform X3
XM_015474918.1	XP_015330404.1	MAP kinase-activating death domain protein isoform X23
NM_001193078.1	NP_001180007.1	MAP kinase-activating death domain protein
XM_015474911.2	XP_015330397.1	MAP kinase-activating death domain protein isoform X18
XM_015474918.2	XP_015330404.1	MAP kinase-activating death domain protein isoform X25
XM_015474921.1	XP_015330407.1	MAP kinase-activating death domain protein isoform X28
XM_015474893.1	XP_015330379.1	MAP kinase-activating death domain protein isoform X1
XM_015474903.1	XP_015330389.1	MAP kinase-activating death domain protein isoform X8
XM_015474904.1	XP_015330390.1	MAP kinase-activating death domain protein isoform X9
XM_015474906.1	XP_015330392.1	MAP kinase-activating death domain protein isoform X11
XM_015474908.1	XP_015330394.1	MAP kinase-activating death domain protein isoform X13
XM_015474913.1	XP_015330399.1	MAP kinase-activating death domain protein isoform X18
XM_015474920.1	XP_015330406.1	MAP kinase-activating death domain protein isoform X25
XM_024975352.1	XP_024831120.1	MAP kinase-activating death domain protein isoform X1
XM_024975363.1	XP_024831131.1	MAP kinase-activating death domain protein isoform X11
XM_015474910.1	XP_015330396.1	MAP kinase-activating death domain protein isoform X12
XM_015474912.1	XP_015330398.1	MAP kinase-activating death domain protein isoform X19
XM_024975370.1	XP_024831138.1	MAP kinase-activating death domain protein isoform X20
XM_015474916.1	XP_015330402.1	MAP kinase-activating death domain protein isoform X23
XM_024975372.1	XP_024831140.1	MAP kinase-activating death domain protein isoform X24
XM_015474900.1	XP_015330386.1	MAP kinase-activating death domain protein isoform X4
XM_015474901.1	XP_015330387.1	MAP kinase-activating death domain protein isoform X5
XM_010812836.2	XP_010811138.1	MAP kinase-activating death domain protein isoform X6
XM_015474902.1	XP_015330388.1	MAP kinase-activating death domain protein isoform X7
XM_015474905.1	XP_015330391.1	MAP kinase-activating death domain protein isoform X10
XM_015474911.1	XP_015330397.1	MAP kinase-activating death domain protein isoform X16
XM_015474914.1	XP_015330400.1	MAP kinase-activating death domain protein isoform X19
XM_024975349.1	XP_024831117.1	MAP kinase-activating death domain protein isoform X1
XM_024975354.1	XP_024831122.1	MAP kinase-activating death domain protein isoform X3
XM_024975358.1	XP_024831126.1	MAP kinase-activating death domain protein isoform X7
XM_024975359.1	XP_024831127.1	MAP kinase-activating death domain protein isoform X8
XM_024975362.1	XP_024831130.1	MAP kinase-activating death domain protein isoform X10
XM_024975365.1	XP_024831133.1	MAP kinase-activating death domain protein isoform X14
XM_024975366.1	XP_024831134.1	MAP kinase-activating death domain protein isoform X15
XM_024975368.1	XP_024831136.1	MAP kinase-activating death domain protein isoform X17
XM_015474895.1	XP_015330381.1	MAP kinase-activating death domain protein isoform X1
XM_015474896.1	XP_015330382.1	MAP kinase-activating death domain protein isoform X1
XM_015474917.1	XP_015330403.1	MAP kinase-activating death domain protein isoform X22
XM_024975348.1	XP_024831116.1	MAP kinase-activating death domain protein isoform X1
XM_024975351.1	XP_024831119.1	MAP kinase-activating death domain protein isoform X1
XM_024975353.1	XP_024831121.1	MAP kinase-activating death domain protein isoform X2
XM_024975355.1	XP_024831123.1	MAP kinase-activating death domain protein isoform X4
XM_024975356.1	XP_024831124.1	MAP kinase-activating death domain protein isoform X5
XM_024975360.1	XP_024831128.1	MAP kinase-activating death domain protein isoform X9
XM_024975367.1	XP_024831135.1	MAP kinase-activating death domain protein isoform X16
XM_024975371.1	XP_024831139.1	MAP kinase-activating death domain protein isoform X22
XM_015474920.2	XP_015330406.1	MAP kinase-activating death domain protein isoform X27

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222853	XM_024975351.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222853	XM_024975349.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222853	XM_024975352.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222853	XM_024975348.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222854	XM_024975353.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222855	XM_024975354.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222856	XM_024975355.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222857	XM_024975356.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222858	XM_024975358.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222859	XM_024975359.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222860	XM_024975360.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222861	XM_024975362.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222862	XM_024975363.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222863	XM_024975364.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222864	XM_024975365.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222865	XM_024975366.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222866	XM_024975367.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222867	XM_024975368.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222868	XM_024975371.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa92805	XM_015474893.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92805	XM_015474895.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92805	XM_015474897.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92805	XM_015474894.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92805	XM_015474896.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92806	XM_015474898.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92807	XM_015474899.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92808	XM_015474900.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92809	XM_015474901.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92810	XM_015474902.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92811	XM_015474903.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92812	XM_015474904.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92813	XM_015474905.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92814	XM_015474906.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92815	XM_015474908.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92816	XM_015474909.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92817	XM_015474913.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa26755	NM_001193078.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26757	XM_015474907.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26757	XM_024975357.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26759	XM_015474910.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26760	XM_015474911.2 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26761	XM_015474912.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26762	XM_015474914.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26762	XM_024975370.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26763	XM_015474915.2 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26764	XM_015474916.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26765	XM_024975372.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26765	XM_015474917.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26766	XM_015474918.2 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26767	XM_015474919.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26768	XM_015474920.2 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26769	XM_015474921.1 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52688	XM_010812836.2 Latest version!	Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa26763	XM_015474915.1	Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26766	XM_015474918.1	Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26760	XM_015474911.1	Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa26768	XM_015474920.1	Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADD ( XM_024975352.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831120.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa222853
Clone ID Related Accession (Same CDS sequence)	XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
Accession Version	XM_024975352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831120.1
CDS	231..5180
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975352.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975352.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975349.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831117.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OBa222853
Clone ID Related Accession (Same CDS sequence)	XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
Accession Version	XM_024975349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831117.1
CDS	235..5184
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975349.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975349.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975348.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831116.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

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CloneID	OBa222853
Clone ID Related Accession (Same CDS sequence)	XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
Accession Version	XM_024975348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831116.1
CDS	238..5187
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975348.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975348.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975351.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831119.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OBa222853
Clone ID Related Accession (Same CDS sequence)	XM_024975352.1 , XM_024975349.1 , XM_024975348.1 , XM_024975351.1
Accession Version	XM_024975351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831119.1
CDS	281..5230
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975351.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975351.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975353.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831121.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa222854
Clone ID Related Accession (Same CDS sequence)	XM_024975353.1
Accession Version	XM_024975353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC 
TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG 
ACCCCGCCCC GGCCCGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831121.1
CDS	238..5184
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975353.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975353.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC 
TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG 
ACCCCGCCCC GGCCCGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975354.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831122.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa222855
Clone ID Related Accession (Same CDS sequence)	XM_024975354.1
Accession Version	XM_024975354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831122.1
CDS	238..5175
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975354.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975354.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975355.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831123.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OBa222856
Clone ID Related Accession (Same CDS sequence)	XM_024975355.1
Accession Version	XM_024975355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG 
CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA 
TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG 
CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC 
TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG 
AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG 
AAGAGCACGC CCTTCCCCAG TCTGAAAGTA TTTGGGCTAA ATACTCTAAT GGAGATTGTT 
ACTGAAGCCG GCCCCGGGAG TGGTGAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG 
TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC 
AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC 
CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG 
CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG 
GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC 
AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC 
AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG 
CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT 
GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA 
CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC 
TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG 
GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA 
AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT 
CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT 
GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC 
AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA 
GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA 
GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT 
GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG 
ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG 
AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG 
GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG 
CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG 
GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA 
GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC 
TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC 
GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG 
TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT 
CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC 
ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG 
GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG 
ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC 
AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831123.1
CDS	238..5145
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975355.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975355.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG 
CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA 
TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG 
CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC 
TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG 
AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG 
AAGAGCACGC CCTTCCCCAG TCTGAAAGTA TTTGGGCTAA ATACTCTAAT GGAGATTGTT 
ACTGAAGCCG GCCCCGGGAG TGGTGAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG 
TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC 
AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC 
CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG 
CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG 
GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC 
AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC 
AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG 
CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT 
GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA 
CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC 
TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG 
GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA 
AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT 
CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT 
GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC 
AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA 
GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA 
GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT 
GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG 
ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG 
AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG 
GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG 
CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG 
GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA 
GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC 
TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC 
GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG 
TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT 
CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC 
ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG 
GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG 
ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC 
AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975356.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831124.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OBa222857
Clone ID Related Accession (Same CDS sequence)	XM_024975356.1
Accession Version	XM_024975356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCGAT GGCCCACGAG 
ATCTGCTACT CCGTGTTGTG TCTCTTCTCG TACGTGGCTG CCGTCCGCAG CAGTGAGGAG 
GACCTCAGGA CCCCGCCCCG GCCCGTCTCT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831124.1
CDS	238..5133
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975356.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975356.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCGAT GGCCCACGAG 
ATCTGCTACT CCGTGTTGTG TCTCTTCTCG TACGTGGCTG CCGTCCGCAG CAGTGAGGAG 
GACCTCAGGA CCCCGCCCCG GCCCGTCTCT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831126.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OBa222858
Clone ID Related Accession (Same CDS sequence)	XM_024975358.1
Accession Version	XM_024975358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC 
TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG 
ACCCCGCCCC GGCCCGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831126.1
CDS	238..5124
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975358.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCGA TGGCCCACGA GATCTGCTAC 
TCCGTGTTGT GTCTCTTCTC GTACGTGGCT GCCGTCCGCA GCAGTGAGGA GGACCTCAGG 
ACCCCGCCCC GGCCCGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975359.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831127.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OBa222859
Clone ID Related Accession (Same CDS sequence)	XM_024975359.1
Accession Version	XM_024975359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831127.1
CDS	238..5121
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975359.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975360.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831128.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa222860
Clone ID Related Accession (Same CDS sequence)	XM_024975360.1
Accession Version	XM_024975360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG 
CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA 
TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG 
CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC 
TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG 
AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG 
AAGAGCACGC CCTTCCCCAG TCTGAAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG 
TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC 
AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC 
CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG 
CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG 
GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC 
AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC 
AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG 
CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT 
GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA 
CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC 
TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG 
GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA 
AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT 
CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT 
GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC 
AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA 
GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA 
GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT 
GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG 
ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG 
AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG 
GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG 
CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG 
GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA 
GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC 
TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC 
GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG 
TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT 
CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC 
ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG 
GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG 
ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC 
AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831128.1
CDS	238..5085
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975360.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975360.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GTCAGTGCGC CAGCGAGCCT CTGACAGCCC GTGCCTCCAG 
CCCCAGTGTG GCTTTCCCCC TGAGGAAGAC GATGAGCAGG GTGAAAGTTA CACCCCCCGA 
TTCAGCCAGC ATGTCAGTGG CCATCGGGCT CAAAAGCTGC TGCGGCCCAG CAGCTTGAAG 
CTGGCAAGTG ACTCAGACGC AGAGTCGGAC TCTCGAGCGA GCTCGCCCAC CTCCACTGTC 
TCCAGCAACA GCACGGAGGG CTTCGGGGGC ATCATGTCTT TGGCCAGCAG CCTGTATCGG 
AACCACAGCA CCAGCTTCAG CCTTTCGAAC CTCACACTGC CCACCAAAGG AGCCCGAGAG 
AAGAGCACGC CCTTCCCCAG TCTGAAAGGG CACAGGCGGG CGTTGGTGGA CCAGAAGTCG 
TCCGTCATTA AACACAGCCC GACCGTGAAG AGAGAGCCCC CGTCACCCCA GGGCCGAGCC 
AGCAACTCTA GTGAGAACCA GCAGTTCCTG AAGGAGGTGG TGCACAGCGT GCTGGACGGC 
CAGGGCGTCG GCTGGCTCAA CATGAAGAAG GTGCGGCGGC TGCTGGAGAG CGAGCAGCTG 
CGCGTCTTCG TCCTGAGCAA GCTGAACCGC ACGGTGCAGT CCGAGGACGA GGCCCGGCAG 
GATGTCATCC CCGACGTGGA GATCAGCCGG AAGGTGTACA AGGGCATGTT GGATCTGCTC 
AAGTGCACAG TCCTCAGTCT GGAGCAGTCC TTTGCCCACG CTGGCCTGGG TGGCATGGCC 
AGCATCTTCG GGCTTCTGGA GATCGCCCAG ACCCACTACT ACAGTAAAGA ACCAGACAAG 
CGGAAGAGAA GTCCCACAGA GAGCATCAGT ACACCAGTCG GCAAGGATCC TGGCCTGGCT 
GGGCGGGGGG ACCCAAAGGC CATGGCACAG CTGAGGGTCC CCCAGCTGGG ACCTCTGGCA 
CCGAGTGCCC CAGGAAAGAG TCCCAAAGAA CTGGACACCA GAAGTCTAAA GGAAGAGAAC 
TTTGTGGCAT CTATCGAATT GTGGAGCAAG CACCAGGAAG TGAAAAATCA AAAAGCTTTG 
GAAAAACAGA GGCCTGAAGT AATCAAACCC ACCTTTGACC TTGGTGAGAC CGACGAGAAA 
AAGTCCCAGG TCAGCGCAGA CAGTGGTGTG AGCCTGACAT CTGGTTCCCA GAGGACCGAT 
CCAGACTCTG TCATCAGTGT GAGTCCCGCT GTGATGATCC GAAGCTCAAG TCAGGACTCT 
GAAGTTAGCA CCGTGGTGAG CAACAGCTCC GGAGAGACCC TGGGAGCAGA CAGCGACCTC 
AGCAGCAGTG CGGGTGATGG CCCAGGCGGC GAGGGCAGCG CCTACTTGGC AGGCTCTCGA 
GGCACCTTGT CTGACAGCGA GATCGAGACC AACTCTGCCA CCAGTGCCAT CTTTGGTAAA 
GCCCATAGCT TGAAGCCAAG TGTGAAGGAG AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT 
GAAGACGTCA GCCAGCGAGT CTACCTCTAC GAGGGACTCC TAGGAAGGGA CAAAGGATCG 
ATGTGGGACC AGTTAGAGGA CGCTGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA GTTCTGGGAA GACGCGTTCT TAGATGCCGT GATGTTGGAG 
AGAGAAGGAA TGGGCATGGA CCAGGGTCCC CAGGAAATGA TAGACAGGTA CCTGTCCCTG 
GGAGAACACG ACCGGAAGCG CCTGGAGGAC GATGAAGACC GTCTGCTGGC CACACTCTTG 
CACAACCTCA TCTCGTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGGAAGAAG 
GTGAGGCGCC TGATGGGAAA GTCCCATATT GGGCTTGTGT ACAGTCAGCA AATCAATGAA 
GTCCTTGACC AGCTGGCCAA CCTGAATGGA CGCGATCTCT CCATCCGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTCGTGGTA CATGCAGGGA CAGACACGAA TGGAGATATC 
TTTTTCATGG AGGTGTGTGA CGACTGCGTG GTGCTGCGCA GTAACATCGG GACGGTGTAC 
GAGCGCTGGT GGTACGAGAA ACTCATCAAC ATGACCTACT GCCCCAAGAC CAAGGTGCTG 
TGCTTGTGGC GCAGAAATGG CTCCGAGACC CAGCTCAACA AGTTCTATAC TAAGAAGTGT 
CGGGAGCTGT ACTACTGCGT GAAGGACAGC ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC 
ATCAAGCCGG GGCCGGAATT GGGTGGCGAG TTCCCCGTGC AGGACATGAA GACTGGCGAG 
GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG ATCAACCTCA AGTTCATGCA CAACCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTCCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCG 
ATGGCCCACG AGATCTGCTA CTCCGTGTTG TGTCTCTTCT CGTACGTGGC TGCCGTCCGC 
AGCAGTGAGG AGGACCTCAG GACCCCGCCC CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975362.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831130.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa222861
Clone ID Related Accession (Same CDS sequence)	XM_024975362.1
Accession Version	XM_024975362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC 
GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG 
GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831130.1
CDS	238..5076
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975362.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975362.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC 
GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG 
GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975363.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831131.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OBa222862
Clone ID Related Accession (Same CDS sequence)	XM_024975363.1
Accession Version	XM_024975363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC 
TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC 
CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831131.1
CDS	1..4833
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975363.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC 
TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC 
CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975364.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831132.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OBa222863
Clone ID Related Accession (Same CDS sequence)	XM_024975364.1
Accession Version	XM_024975364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGAATTGTGG 
AGCAAGCACC AGGAAGTGAA AAATCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCCACCT TTGACCTTGG TGAGACCGAC GAGAAAAAGT CCCAGGTCAG CGCAGACAGT 
GGTGTGAGCC TGACATCTGG TTCCCAGAGG ACCGATCCAG ACTCTGTCAT CAGTGTGAGT 
CCCGCTGTGA TGATCCGAAG CTCAAGTCAG GACTCTGAAG TTAGCACCGT GGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831132.1
CDS	238..5061
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975364.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975364.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGAATTGTGG 
AGCAAGCACC AGGAAGTGAA AAATCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCCACCT TTGACCTTGG TGAGACCGAC GAGAAAAAGT CCCAGGTCAG CGCAGACAGT 
GGTGTGAGCC TGACATCTGG TTCCCAGAGG ACCGATCCAG ACTCTGTCAT CAGTGTGAGT 
CCCGCTGTGA TGATCCGAAG CTCAAGTCAG GACTCTGAAG TTAGCACCGT GGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975365.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831133.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OBa222864
Clone ID Related Accession (Same CDS sequence)	XM_024975365.1
Accession Version	XM_024975365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT 
CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG 
CCCGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831133.1
CDS	1..4815
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975365.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975365.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT 
CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG 
CCCGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975366.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831134.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa222865
Clone ID Related Accession (Same CDS sequence)	XM_024975366.1
Accession Version	XM_024975366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC CGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGTCTGCT GGCCACACTC TTGCACAACC TCATCTCGTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGCGATC TCTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGGTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA TGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ATTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGC GAGGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCGATGGCC CACGAGATCT GCTACTCCGT GTTGTGTCTC 
TTCTCGTACG TGGCTGCCGT CCGCAGCAGT GAGGAGGACC TCAGGACCCC GCCCCGGCCC 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831134.1
CDS	238..5049
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975366.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975366.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG TGATGGCCCA 
GGCGGCGAGG GCAGCGCCTA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGCGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTG 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TTTTCTGAAG ACGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC CGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGTCTGCT GGCCACACTC TTGCACAACC TCATCTCGTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGCGATC TCTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGGTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA TGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ATTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGC GAGGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCGATGGCC CACGAGATCT GCTACTCCGT GTTGTGTCTC 
TTCTCGTACG TGGCTGCCGT CCGCAGCAGT GAGGAGGACC TCAGGACCCC GCCCCGGCCC 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975367.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831135.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa222866
Clone ID Related Accession (Same CDS sequence)	XM_024975367.1
Accession Version	XM_024975367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GGCTCAAAAG CTGCTGCGGC CCAGCAGCTT GAAGCTGGCA 
AGTGACTCAG ACGCAGAGTC GGACTCTCGA GCGAGCTCGC CCACCTCCAC TGTCTCCAGC 
AACAGCACGG AGGGCTTCGG GGGCATCATG TCTTTGGCCA GCAGCCTGTA TCGGAACCAC 
AGCACCAGCT TCAGCCTTTC GAACCTCACA CTGCCCACCA AAGGAGCCCG AGAGAAGAGC 
ACGCCCTTCC CCAGTCTGAA AGTATTTGGG CTAAATACTC TAATGGAGAT TGTTACTGAA 
GCCGGCCCCG GGAGTGGTGA AGGGCACAGG CGGGCGTTGG TGGACCAGAA GTCGTCCGTC 
ATTAAACACA GCCCGACCGT GAAGAGAGAG CCCCCGTCAC CCCAGGGCCG AGCCAGCAAC 
TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGC 
GTCGGCTGGC TCAACATGAA GAAGGTGCGG CGGCTGCTGG AGAGCGAGCA GCTGCGCGTC 
TTCGTCCTGA GCAAGCTGAA CCGCACGGTG CAGTCCGAGG ACGAGGCCCG GCAGGATGTC 
ATCCCCGACG TGGAGATCAG CCGGAAGGTG TACAAGGGCA TGTTGGATCT GCTCAAGTGC 
ACAGTCCTCA GTCTGGAGCA GTCCTTTGCC CACGCTGGCC TGGGTGGCAT GGCCAGCATC 
TTCGGGCTTC TGGAGATCGC CCAGACCCAC TACTACAGTA AAGAACCAGA CAAGCGGAAG 
AGAAGTCCCA CAGAGAGCAT CAGTACACCA GTCGGCAAGG ATCCTGGCCT GGCTGGGCGG 
GGGGACCCAA AGGCCATGGC ACAGCTGAGG GTCCCCCAGC TGGGACCTCT GGCACCGAGT 
GCCCCAGGAA AGAGTCCCAA AGAACTGGAC ACCAGAAGTC TAAAGGAAGA GAACTTTGTG 
GCATCTATCG AATTGTGGAG CAAGCACCAG GAAGTGAAAA ATCAAAAAGC TTTGGAAAAA 
CAGAGGCCTG AAGTAATCAA ACCCACCTTT GACCTTGGTG AGACCGACGA GAAAAAGTCC 
CAGGTCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC CGATCCAGAC 
TCTGTCATCA GTGTGAGTCC CGCTGTGATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGCAACAG CTCCGGAGAG ACCCTGGGAG CAGACAGCGA CCTCAGCAGC 
AGTGCGGGTG ATGGCCCAGG CGGCGAGGGC AGCGCCTACT TGGCAGGCTC TCGAGGCACC 
TTGTCTGACA GCGAGATCGA GACCAACTCT GCCACCAGTG CCATCTTTGG TAAAGCCCAT 
AGCTTGAAGC CAAGTGTGAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTT TTCTGAAGAC 
GTCAGCCAGC GAGTCTACCT CTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGACGCTGC TATGGAGACC TTTTCTATAA GCAAAGAGCG TTCTACTTTA 
TGGGACCAAA TGCAGTTCTG GGAAGACGCG TTCTTAGATG CCGTGATGTT GGAGAGAGAA 
GGAATGGGCA TGGACCAGGG TCCCCAGGAA ATGATAGACA GGTACCTGTC CCTGGGAGAA 
CACGACCGGA AGCGCCTGGA GGACGATGAA GACCGTCTGC TGGCCACACT CTTGCACAAC 
CTCATCTCGT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTGATGG GAAAGTCCCA TATTGGGCTT GTGTACAGTC AGCAAATCAA TGAAGTCCTT 
GACCAGCTGG CCAACCTGAA TGGACGCGAT CTCTCCATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAGAAGC AGACATTCGT GGTACATGCA GGGACAGACA CGAATGGAGA TATCTTTTTC 
ATGGAGGTGT GTGACGACTG CGTGGTGCTG CGCAGTAACA TCGGGACGGT GTACGAGCGC 
TGGTGGTACG AGAAACTCAT CAACATGACC TACTGCCCCA AGACCAAGGT GCTGTGCTTG 
TGGCGCAGAA ATGGCTCCGA GACCCAGCTC AACAAGTTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GCGTGAAGGA CAGCATGGAG CGCGCCGCCG CCCGCCAGCA GAGCATCAAG 
CCGGGGCCGG AATTGGGTGG CGAGTTCCCC GTGCAGGACA TGAAGACTGG CGAGGGGGGC 
TTGCTGCAGG TCACCCTCGA AGGGATCAAC CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCGATGGCC 
CACGAGATCT GCTACTCCGT GTTGTGTCTC TTCTCGTACG TGGCTGCCGT CCGCAGCAGT 
GAGGAGGACC TCAGGACCCC GCCCCGGCCC GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831135.1
CDS	238..5019
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975367.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975367.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGAACC TGTTGACTCA GCAGAGACCG ACGATAAGGC GGCAGGAGGC 
GTCCCCAAGT CCGTACCCAC CGTGCCTCCC GGCATGGGCA AGTGCAGCAT GGACAGGCAT 
CAGACAGAAA CCGGAGAGGG GGCTCAAAAG CTGCTGCGGC CCAGCAGCTT GAAGCTGGCA 
AGTGACTCAG ACGCAGAGTC GGACTCTCGA GCGAGCTCGC CCACCTCCAC TGTCTCCAGC 
AACAGCACGG AGGGCTTCGG GGGCATCATG TCTTTGGCCA GCAGCCTGTA TCGGAACCAC 
AGCACCAGCT TCAGCCTTTC GAACCTCACA CTGCCCACCA AAGGAGCCCG AGAGAAGAGC 
ACGCCCTTCC CCAGTCTGAA AGTATTTGGG CTAAATACTC TAATGGAGAT TGTTACTGAA 
GCCGGCCCCG GGAGTGGTGA AGGGCACAGG CGGGCGTTGG TGGACCAGAA GTCGTCCGTC 
ATTAAACACA GCCCGACCGT GAAGAGAGAG CCCCCGTCAC CCCAGGGCCG AGCCAGCAAC 
TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGC 
GTCGGCTGGC TCAACATGAA GAAGGTGCGG CGGCTGCTGG AGAGCGAGCA GCTGCGCGTC 
TTCGTCCTGA GCAAGCTGAA CCGCACGGTG CAGTCCGAGG ACGAGGCCCG GCAGGATGTC 
ATCCCCGACG TGGAGATCAG CCGGAAGGTG TACAAGGGCA TGTTGGATCT GCTCAAGTGC 
ACAGTCCTCA GTCTGGAGCA GTCCTTTGCC CACGCTGGCC TGGGTGGCAT GGCCAGCATC 
TTCGGGCTTC TGGAGATCGC CCAGACCCAC TACTACAGTA AAGAACCAGA CAAGCGGAAG 
AGAAGTCCCA CAGAGAGCAT CAGTACACCA GTCGGCAAGG ATCCTGGCCT GGCTGGGCGG 
GGGGACCCAA AGGCCATGGC ACAGCTGAGG GTCCCCCAGC TGGGACCTCT GGCACCGAGT 
GCCCCAGGAA AGAGTCCCAA AGAACTGGAC ACCAGAAGTC TAAAGGAAGA GAACTTTGTG 
GCATCTATCG AATTGTGGAG CAAGCACCAG GAAGTGAAAA ATCAAAAAGC TTTGGAAAAA 
CAGAGGCCTG AAGTAATCAA ACCCACCTTT GACCTTGGTG AGACCGACGA GAAAAAGTCC 
CAGGTCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC CGATCCAGAC 
TCTGTCATCA GTGTGAGTCC CGCTGTGATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGCAACAG CTCCGGAGAG ACCCTGGGAG CAGACAGCGA CCTCAGCAGC 
AGTGCGGGTG ATGGCCCAGG CGGCGAGGGC AGCGCCTACT TGGCAGGCTC TCGAGGCACC 
TTGTCTGACA GCGAGATCGA GACCAACTCT GCCACCAGTG CCATCTTTGG TAAAGCCCAT 
AGCTTGAAGC CAAGTGTGAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTT TTCTGAAGAC 
GTCAGCCAGC GAGTCTACCT CTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGACGCTGC TATGGAGACC TTTTCTATAA GCAAAGAGCG TTCTACTTTA 
TGGGACCAAA TGCAGTTCTG GGAAGACGCG TTCTTAGATG CCGTGATGTT GGAGAGAGAA 
GGAATGGGCA TGGACCAGGG TCCCCAGGAA ATGATAGACA GGTACCTGTC CCTGGGAGAA 
CACGACCGGA AGCGCCTGGA GGACGATGAA GACCGTCTGC TGGCCACACT CTTGCACAAC 
CTCATCTCGT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTGATGG GAAAGTCCCA TATTGGGCTT GTGTACAGTC AGCAAATCAA TGAAGTCCTT 
GACCAGCTGG CCAACCTGAA TGGACGCGAT CTCTCCATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAGAAGC AGACATTCGT GGTACATGCA GGGACAGACA CGAATGGAGA TATCTTTTTC 
ATGGAGGTGT GTGACGACTG CGTGGTGCTG CGCAGTAACA TCGGGACGGT GTACGAGCGC 
TGGTGGTACG AGAAACTCAT CAACATGACC TACTGCCCCA AGACCAAGGT GCTGTGCTTG 
TGGCGCAGAA ATGGCTCCGA GACCCAGCTC AACAAGTTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GCGTGAAGGA CAGCATGGAG CGCGCCGCCG CCCGCCAGCA GAGCATCAAG 
CCGGGGCCGG AATTGGGTGG CGAGTTCCCC GTGCAGGACA TGAAGACTGG CGAGGGGGGC 
TTGCTGCAGG TCACCCTCGA AGGGATCAAC CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCGATGGCC 
CACGAGATCT GCTACTCCGT GTTGTGTCTC TTCTCGTACG TGGCTGCCGT CCGCAGCAGT 
GAGGAGGACC TCAGGACCCC GCCCCGGCCC GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975368.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831136.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OBa222867
Clone ID Related Accession (Same CDS sequence)	XM_024975368.1
Accession Version	XM_024975368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC 
TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC 
CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831136.1
CDS	1..4773
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975368.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975368.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCGATGGC CCACGAGATC 
TGCTACTCCG TGTTGTGTCT CTTCTCGTAC GTGGCTGCCG TCCGCAGCAG TGAGGAGGAC 
CTCAGGACCC CGCCCCGGCC CGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975371.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831139.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

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CloneID	OBa222868
Clone ID Related Accession (Same CDS sequence)	XM_024975371.1
Accession Version	XM_024975371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831139.1
CDS	238..4968
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024975371.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975371.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCCGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GTCTGCTGGC CACACTCTTG CACAACCTCA TCTCGTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGCGATCTCT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGGTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAATGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAATT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGCGAG GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474894.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330380.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OBa92805
Clone ID Related Accession (Same CDS sequence)	XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
Accession Version	XM_015474894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330380.1
CDS	358..5325
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474894.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474894.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474897.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330383.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa92805
Clone ID Related Accession (Same CDS sequence)	XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
Accession Version	XM_015474897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330383.1
CDS	713..5680
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474897.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474897.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474893.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330379.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92805
Clone ID Related Accession (Same CDS sequence)	XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
Accession Version	XM_015474893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330379.1
CDS	274..5241
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474893.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474893.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474895.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330381.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OBa92805
Clone ID Related Accession (Same CDS sequence)	XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
Accession Version	XM_015474895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330381.1
CDS	512..5479
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474895.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474895.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474896.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330382.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OBa92805
Clone ID Related Accession (Same CDS sequence)	XM_015474894.1 , XM_015474897.1 , XM_015474893.1 , XM_015474895.1 , XM_015474896.1
Accession Version	XM_015474896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330382.1
CDS	282..5249
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474896.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474896.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474898.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330384.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OBa92806
Clone ID Related Accession (Same CDS sequence)	XM_015474898.1
Accession Version	XM_015474898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG 
AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330384.1
CDS	274..5238
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474898.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474898.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG 
AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474899.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330385.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OBa92807
Clone ID Related Accession (Same CDS sequence)	XM_015474899.1
Accession Version	XM_015474899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC 
GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG 
GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330385.1
CDS	273..5231
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474899.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474899.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC GATGGCCCAC 
GAGATCTGCT ACTCCGTGTT GTGTCTCTTC TCGTACGTGG CTGCCGTCCG CAGCAGTGAG 
GAGGACCTCA GGACCCCGCC CCGGCCCGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474900.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330386.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa92808
Clone ID Related Accession (Same CDS sequence)	XM_015474900.1
Accession Version	XM_015474900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGAAG CGCCTCTGCA GAGAGGTCAG GAGGAGCAGG 
AGCGTAGGGA GGATGAGAGA AGGGAGGCAG GAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330386.1
CDS	274..5232
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474900.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474900.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGAAG CGCCTCTGCA GAGAGGTCAG GAGGAGCAGG 
AGCGTAGGGA GGATGAGAGA AGGGAGGCAG GAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474901.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330387.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa92809
Clone ID Related Accession (Same CDS sequence)	XM_015474901.1
Accession Version	XM_015474901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGAAGCGC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330387.1
CDS	274..5229
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474901.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474901.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGAAGCGC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474902.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330388.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

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CloneID	OBa92810
Clone ID Related Accession (Same CDS sequence)	XM_015474902.1
Accession Version	XM_015474902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG 
GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG 
GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG 
TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC 
ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC 
AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC 
TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC 
CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA 
GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG 
GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGGC ACAGGCGGGC GTTGGTGGAC 
CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG 
GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC 
GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG 
GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG 
GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT 
GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT 
GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA 
CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG 
GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC 
GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG 
AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT 
CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC 
AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA 
GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC 
TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT 
CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC 
AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG 
ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC 
CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC 
ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA 
ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT 
GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG 
ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC 
AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT 
AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC 
CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG 
ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC 
AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA 
GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA 
CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC 
CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330388.1
CDS	274..5199
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474902.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474902.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG 
GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG 
GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG 
TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC 
ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC 
AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC 
TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC 
CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA 
GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG 
GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGGC ACAGGCGGGC GTTGGTGGAC 
CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG 
GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC 
GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG 
GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG 
GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT 
GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT 
GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA 
CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG 
GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC 
GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG 
AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT 
CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC 
AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA 
GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC 
TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT 
CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC 
AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG 
ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC 
CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC 
ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA 
ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT 
GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG 
ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC 
AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT 
AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC 
CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG 
ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC 
AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA 
GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA 
CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC 
CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474903.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330389.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

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CloneID	OBa92811
Clone ID Related Accession (Same CDS sequence)	XM_015474903.1
Accession Version	XM_015474903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG 
TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA 
GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA 
GTTAGCACCG TGGTGAGCAA CAGCTCCGGA GAGACCCTGG GAGCAGACAG CGACCTCAGC 
AGCAGTGCGG GTGATGGCCC AGGCGGCGAG GGCAGCGCCT ACTTGGCAGG CTCTCGAGGC 
ACCTTGTCTG ACAGCGAGAT CGAGACCAAC TCTGCCACCA GTGCCATCTT TGGTAAAGCC 
CATAGCTTGA AGCCAAGTGT GAAGGAGAAG CTGGTGGGCA GCCCAGTTCG CTTTTCTGAA 
GACGTCAGCC AGCGAGTCTA CCTCTACGAG GGACTCCTAG GAAGGGACAA AGGATCGATG 
TGGGACCAGT TAGAGGACGC TGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT 
TTATGGGACC AAATGCAGTT CTGGGAAGAC GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA 
GAAGGAATGG GCATGGACCA GGGTCCCCAG GAAATGATAG ACAGGTACCT GTCCCTGGGA 
GAACACGACC GGAAGCGCCT GGAGGACGAT GAAGACCGTC TGCTGGCCAC ACTCTTGCAC 
AACCTCATCT CGTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG GAAGAAGGTG 
AGGCGCCTGA TGGGAAAGTC CCATATTGGG CTTGTGTACA GTCAGCAAAT CAATGAAGTC 
CTTGACCAGC TGGCCAACCT GAATGGACGC GATCTCTCCA TCCGGTCCAG TGGCAGCCGG 
CACATGAAGA AGCAGACATT CGTGGTACAT GCAGGGACAG ACACGAATGG AGATATCTTT 
TTCATGGAGG TGTGTGACGA CTGCGTGGTG CTGCGCAGTA ACATCGGGAC GGTGTACGAG 
CGCTGGTGGT ACGAGAAACT CATCAACATG ACCTACTGCC CCAAGACCAA GGTGCTGTGC 
TTGTGGCGCA GAAATGGCTC CGAGACCCAG CTCAACAAGT TCTATACTAA GAAGTGTCGG 
GAGCTGTACT ACTGCGTGAA GGACAGCATG GAGCGCGCCG CCGCCCGCCA GCAGAGCATC 
AAGCCGGGGC CGGAATTGGG TGGCGAGTTC CCCGTGCAGG ACATGAAGAC TGGCGAGGGG 
GGCTTGCTGC AGGTCACCCT CGAAGGGATC AACCTCAAGT TCATGCACAA CCAGGAGCGG 
AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTCCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGGTAATTA CACTATTTTG CTCTTAGGTC TGGACTCACA TGGCAGTAAC 
TCAAACCTCG GAGCTCCAGA GGAGGGACTA GAGACAGAGA AGAAGCGCCT CTGCAGAGAG 
GTCAGGAGGA GCAGGAGCGT AGGGAGGATG AGAGAAGGGA GGCAGGAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330389.1
CDS	273..5186
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474903.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474903.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG 
TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA 
GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA 
GTTAGCACCG TGGTGAGCAA CAGCTCCGGA GAGACCCTGG GAGCAGACAG CGACCTCAGC 
AGCAGTGCGG GTGATGGCCC AGGCGGCGAG GGCAGCGCCT ACTTGGCAGG CTCTCGAGGC 
ACCTTGTCTG ACAGCGAGAT CGAGACCAAC TCTGCCACCA GTGCCATCTT TGGTAAAGCC 
CATAGCTTGA AGCCAAGTGT GAAGGAGAAG CTGGTGGGCA GCCCAGTTCG CTTTTCTGAA 
GACGTCAGCC AGCGAGTCTA CCTCTACGAG GGACTCCTAG GAAGGGACAA AGGATCGATG 
TGGGACCAGT TAGAGGACGC TGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT 
TTATGGGACC AAATGCAGTT CTGGGAAGAC GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA 
GAAGGAATGG GCATGGACCA GGGTCCCCAG GAAATGATAG ACAGGTACCT GTCCCTGGGA 
GAACACGACC GGAAGCGCCT GGAGGACGAT GAAGACCGTC TGCTGGCCAC ACTCTTGCAC 
AACCTCATCT CGTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG GAAGAAGGTG 
AGGCGCCTGA TGGGAAAGTC CCATATTGGG CTTGTGTACA GTCAGCAAAT CAATGAAGTC 
CTTGACCAGC TGGCCAACCT GAATGGACGC GATCTCTCCA TCCGGTCCAG TGGCAGCCGG 
CACATGAAGA AGCAGACATT CGTGGTACAT GCAGGGACAG ACACGAATGG AGATATCTTT 
TTCATGGAGG TGTGTGACGA CTGCGTGGTG CTGCGCAGTA ACATCGGGAC GGTGTACGAG 
CGCTGGTGGT ACGAGAAACT CATCAACATG ACCTACTGCC CCAAGACCAA GGTGCTGTGC 
TTGTGGCGCA GAAATGGCTC CGAGACCCAG CTCAACAAGT TCTATACTAA GAAGTGTCGG 
GAGCTGTACT ACTGCGTGAA GGACAGCATG GAGCGCGCCG CCGCCCGCCA GCAGAGCATC 
AAGCCGGGGC CGGAATTGGG TGGCGAGTTC CCCGTGCAGG ACATGAAGAC TGGCGAGGGG 
GGCTTGCTGC AGGTCACCCT CGAAGGGATC AACCTCAAGT TCATGCACAA CCAGGAGCGG 
AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTCCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGGTAATTA CACTATTTTG CTCTTAGGTC TGGACTCACA TGGCAGTAAC 
TCAAACCTCG GAGCTCCAGA GGAGGGACTA GAGACAGAGA AGAAGCGCCT CTGCAGAGAG 
GTCAGGAGGA GCAGGAGCGT AGGGAGGATG AGAGAAGGGA GGCAGGAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474904.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330390.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OBa92812
Clone ID Related Accession (Same CDS sequence)	XM_015474904.1
Accession Version	XM_015474904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330390.1
CDS	274..5181
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474904.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474904.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGGTCAGG 
AGGAGCAGGA GCGTAGGGAG GATGAGAGAA GGGAGGCAGG AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474905.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330391.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OBa92813
Clone ID Related Accession (Same CDS sequence)	XM_015474905.1
Accession Version	XM_015474905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG 
AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330391.1
CDS	274..5178
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474905.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474905.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCCGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGT CTGCTGGCCA CACTCTTGCA CAACCTCATC 
TCGTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG CGATCTCTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGGTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAATGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAATTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGCGAGGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGAAGCGCC TCTGCAGAGA GGTCAGGAGG 
AGCAGGAGCG TAGGGAGGAT GAGAGAAGGG AGGCAGGAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474906.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330392.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa92814
Clone ID Related Accession (Same CDS sequence)	XM_015474906.1
Accession Version	XM_015474906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG 
TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA 
GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA 
GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330392.1
CDS	273..5174
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474906.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474906.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGGGC CTGAAGTAAT CAAACCCACC TTTGACCTTG GTGAGACCGA CGAGAAAAAG 
TCCCAGGTCA GCGCAGACAG TGGTGTGAGC CTGACATCTG GTTCCCAGAG GACCGATCCA 
GACTCTGTCA TCAGTGTGAG TCCCGCTGTG ATGATCCGAA GCTCAAGTCA GGACTCTGAA 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA 
GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474908.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330394.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa92815
Clone ID Related Accession (Same CDS sequence)	XM_015474908.1
Accession Version	XM_015474908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG 
GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG 
GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG 
TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC 
ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC 
AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC 
TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC 
CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA 
GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGGGC ACAGGCGGGC GTTGGTGGAC 
CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG 
GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC 
GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG 
GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG 
GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT 
GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT 
GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA 
CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG 
GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC 
GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG 
AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT 
CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC 
AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA 
GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC 
TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT 
CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC 
AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG 
ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC 
CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC 
ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA 
ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT 
GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG 
ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC 
AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT 
AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC 
CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG 
ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC 
AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA 
GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA 
CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC 
CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330394.1
CDS	274..5139
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474908.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474908.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGAACCT GTTGACTCAG CAGAGACCGA CGATAAGGCG 
GCAGGAGGCG TCCCCAAGTC CGTACCCACC GTGCCTCCCG GCATGGGCAA GTGCAGCATG 
GACAGGCATC AGACAGAAAC CGGAGAGGGG TCAGTGCGCC AGCGAGCCTC TGACAGCCCG 
TGCCTCCAGC CCCAGTGTGG CTTTCCCCCT GAGGAAGACG ATGAGCAGGG TGAAAGTTAC 
ACCCCCCGAT TCAGCCAGCA TGTCAGTGGC CATCGGGCTC AAAAGCTGCT GCGGCCCAGC 
AGCTTGAAGC TGGCAAGTGA CTCAGACGCA GAGTCGGACT CTCGAGCGAG CTCGCCCACC 
TCCACTGTCT CCAGCAACAG CACGGAGGGC TTCGGGGGCA TCATGTCTTT GGCCAGCAGC 
CTGTATCGGA ACCACAGCAC CAGCTTCAGC CTTTCGAACC TCACACTGCC CACCAAAGGA 
GCCCGAGAGA AGAGCACGCC CTTCCCCAGT CTGAAAGGGC ACAGGCGGGC GTTGGTGGAC 
CAGAAGTCGT CCGTCATTAA ACACAGCCCG ACCGTGAAGA GAGAGCCCCC GTCACCCCAG 
GGCCGAGCCA GCAACTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGCGTCGG CTGGCTCAAC ATGAAGAAGG TGCGGCGGCT GCTGGAGAGC 
GAGCAGCTGC GCGTCTTCGT CCTGAGCAAG CTGAACCGCA CGGTGCAGTC CGAGGACGAG 
GCCCGGCAGG ATGTCATCCC CGACGTGGAG ATCAGCCGGA AGGTGTACAA GGGCATGTTG 
GATCTGCTCA AGTGCACAGT CCTCAGTCTG GAGCAGTCCT TTGCCCACGC TGGCCTGGGT 
GGCATGGCCA GCATCTTCGG GCTTCTGGAG ATCGCCCAGA CCCACTACTA CAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCCACAGAG AGCATCAGTA CACCAGTCGG CAAGGATCCT 
GGCCTGGCTG GGCGGGGGGA CCCAAAGGCC ATGGCACAGC TGAGGGTCCC CCAGCTGGGA 
CCTCTGGCAC CGAGTGCCCC AGGAAAGAGT CCCAAAGAAC TGGACACCAG AAGTCTAAAG 
GAAGAGAACT TTGTGGCATC TATCGAATTG TGGAGCAAGC ACCAGGAAGT GAAAAATCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCCA CCTTTGACCT TGGTGAGACC 
GACGAGAAAA AGTCCCAGGT CAGCGCAGAC AGTGGTGTGA GCCTGACATC TGGTTCCCAG 
AGGACCGATC CAGACTCTGT CATCAGTGTG AGTCCCGCTG TGATGATCCG AAGCTCAAGT 
CAGGACTCTG AAGTTAGCAC CGTGGTGAGC AACAGCTCCG GAGAGACCCT GGGAGCAGAC 
AGCGACCTCA GCAGCAGTGC GGGTGATGGC CCAGGCGGCG AGGGCAGCGC CTACTTGGCA 
GGCTCTCGAG GCACCTTGTC TGACAGCGAG ATCGAGACCA ACTCTGCCAC CAGTGCCATC 
TTTGGTAAAG CCCATAGCTT GAAGCCAAGT GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT 
CGCTTTTCTG AAGACGTCAG CCAGCGAGTC TACCTCTACG AGGGACTCCT AGGAAGGGAC 
AAAGGATCGA TGTGGGACCA GTTAGAGGAC GCTGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAG TTCTGGGAAG ACGCGTTCTT AGATGCCGTG 
ATGTTGGAGA GAGAAGGAAT GGGCATGGAC CAGGGTCCCC AGGAAATGAT AGACAGGTAC 
CTGTCCCTGG GAGAACACGA CCGGAAGCGC CTGGAGGACG ATGAAGACCG TCTGCTGGCC 
ACACTCTTGC ACAACCTCAT CTCGTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGGAAGAAGG TGAGGCGCCT GATGGGAAAG TCCCATATTG GGCTTGTGTA CAGTCAGCAA 
ATCAATGAAG TCCTTGACCA GCTGGCCAAC CTGAATGGAC GCGATCTCTC CATCCGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTCGTGGTAC ATGCAGGGAC AGACACGAAT 
GGAGATATCT TTTTCATGGA GGTGTGTGAC GACTGCGTGG TGCTGCGCAG TAACATCGGG 
ACGGTGTACG AGCGCTGGTG GTACGAGAAA CTCATCAACA TGACCTACTG CCCCAAGACC 
AAGGTGCTGT GCTTGTGGCG CAGAAATGGC TCCGAGACCC AGCTCAACAA GTTCTATACT 
AAGAAGTGTC GGGAGCTGTA CTACTGCGTG AAGGACAGCA TGGAGCGCGC CGCCGCCCGC 
CAGCAGAGCA TCAAGCCGGG GCCGGAATTG GGTGGCGAGT TCCCCGTGCA GGACATGAAG 
ACTGGCGAGG GGGGCTTGCT GCAGGTCACC CTCGAAGGGA TCAACCTCAA GTTCATGCAC 
AACCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTCCGA 
GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT TACACTATTT TGCTCTTAGG TCTGGACTCA 
CATGGCAGTA ACTCAAACCT CGGAGCTCCA GAGGAGGGAC TAGAGACAGA GAAGAAGCGC 
CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC GTAGGGAGGA TGAGAGAAGG GAGGCAGGAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474909.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330395.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

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CloneID	OBa92816
Clone ID Related Accession (Same CDS sequence)	XM_015474909.1
Accession Version	XM_015474909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGGCTCAAAA GCTGCTGCGG CCCAGCAGCT TGAAGCTGGC AAGTGACTCA 
GACGCAGAGT CGGACTCTCG AGCGAGCTCG CCCACCTCCA CTGTCTCCAG CAACAGCACG 
GAGGGCTTCG GGGGCATCAT GTCTTTGGCC AGCAGCCTGT ATCGGAACCA CAGCACCAGC 
TTCAGCCTTT CGAACCTCAC ACTGCCCACC AAAGGAGCCC GAGAGAAGAG CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGGCACAG GCGGGCGTTG GTGGACCAGA AGTCGTCCGT CATTAAACAC 
AGCCCGACCG TGAAGAGAGA GCCCCCGTCA CCCCAGGGCC GAGCCAGCAA CTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG CGTCGGCTGG 
CTCAACATGA AGAAGGTGCG GCGGCTGCTG GAGAGCGAGC AGCTGCGCGT CTTCGTCCTG 
AGCAAGCTGA ACCGCACGGT GCAGTCCGAG GACGAGGCCC GGCAGGATGT CATCCCCGAC 
GTGGAGATCA GCCGGAAGGT GTACAAGGGC ATGTTGGATC TGCTCAAGTG CACAGTCCTC 
AGTCTGGAGC AGTCCTTTGC CCACGCTGGC CTGGGTGGCA TGGCCAGCAT CTTCGGGCTT 
CTGGAGATCG CCCAGACCCA CTACTACAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCC 
ACAGAGAGCA TCAGTACACC AGTCGGCAAG GATCCTGGCC TGGCTGGGCG GGGGGACCCA 
AAGGCCATGG CACAGCTGAG GGTCCCCCAG CTGGGACCTC TGGCACCGAG TGCCCCAGGA 
AAGAGTCCCA AAGAACTGGA CACCAGAAGT CTAAAGGAAG AGAACTTTGT GGCATCTATC 
GAATTGTGGA GCAAGCACCA GGAAGTGAAA AATCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCCACCTT TGACCTTGGT GAGACCGACG AGAAAAAGTC CCAGGTCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CCGATCCAGA CTCTGTCATC 
AGTGTGAGTC CCGCTGTGAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA 
GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330395.1
CDS	274..5115
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREVRRSRSVGRMREGRQES

Target ORF information:

RefSeq Version	XM_015474909.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474909.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGGCTCAAAA GCTGCTGCGG CCCAGCAGCT TGAAGCTGGC AAGTGACTCA 
GACGCAGAGT CGGACTCTCG AGCGAGCTCG CCCACCTCCA CTGTCTCCAG CAACAGCACG 
GAGGGCTTCG GGGGCATCAT GTCTTTGGCC AGCAGCCTGT ATCGGAACCA CAGCACCAGC 
TTCAGCCTTT CGAACCTCAC ACTGCCCACC AAAGGAGCCC GAGAGAAGAG CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGGCACAG GCGGGCGTTG GTGGACCAGA AGTCGTCCGT CATTAAACAC 
AGCCCGACCG TGAAGAGAGA GCCCCCGTCA CCCCAGGGCC GAGCCAGCAA CTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG CGTCGGCTGG 
CTCAACATGA AGAAGGTGCG GCGGCTGCTG GAGAGCGAGC AGCTGCGCGT CTTCGTCCTG 
AGCAAGCTGA ACCGCACGGT GCAGTCCGAG GACGAGGCCC GGCAGGATGT CATCCCCGAC 
GTGGAGATCA GCCGGAAGGT GTACAAGGGC ATGTTGGATC TGCTCAAGTG CACAGTCCTC 
AGTCTGGAGC AGTCCTTTGC CCACGCTGGC CTGGGTGGCA TGGCCAGCAT CTTCGGGCTT 
CTGGAGATCG CCCAGACCCA CTACTACAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCC 
ACAGAGAGCA TCAGTACACC AGTCGGCAAG GATCCTGGCC TGGCTGGGCG GGGGGACCCA 
AAGGCCATGG CACAGCTGAG GGTCCCCCAG CTGGGACCTC TGGCACCGAG TGCCCCAGGA 
AAGAGTCCCA AAGAACTGGA CACCAGAAGT CTAAAGGAAG AGAACTTTGT GGCATCTATC 
GAATTGTGGA GCAAGCACCA GGAAGTGAAA AATCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCCACCTT TGACCTTGGT GAGACCGACG AGAAAAAGTC CCAGGTCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CCGATCCAGA CTCTGTCATC 
AGTGTGAGTC CCGCTGTGAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGACGCTG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GGTAATTACA CTATTTTGCT CTTAGGTCTG GACTCACATG GCAGTAACTC AAACCTCGGA 
GCTCCAGAGG AGGGACTAGA GACAGAGAAG AAGCGCCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGATGAG AGAAGGGAGG CAGGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474913.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330399.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

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CloneID	OBa92817
Clone ID Related Accession (Same CDS sequence)	XM_015474913.1
Accession Version	XM_015474913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330399.1
CDS	273..5024
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474913.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474913.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001193078.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180007.1 Bos taurus MAP kinase activating death domain (MADD), mRNA.

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CloneID	OBa26755
Clone ID Related Accession (Same CDS sequence)	NM_001193078.1
Accession Version	NM_001193078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On Jul 17, 2010 this sequence version replaced XM_867337.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG 
TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG 
CCCCGGCCCG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180007.1
CDS	1..4941
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001193078.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193078.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG 
TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG 
CCCCGGCCCG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474907.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330393.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa26757
Clone ID Related Accession (Same CDS sequence)	XM_015474907.1 , XM_024975357.1
Accession Version	XM_015474907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330393.1
CDS	1..4890
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474907.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474907.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975357.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831125.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa26757
Clone ID Related Accession (Same CDS sequence)	XM_015474907.1 , XM_024975357.1
Accession Version	XM_024975357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831125.1
CDS	238..5127
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024975357.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975357.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CGATGGCCCA CGAGATCTGC 
TACTCCGTGT TGTGTCTCTT CTCGTACGTG GCTGCCGTCC GCAGCAGTGA GGAGGACCTC 
AGGACCCCGC CCCGGCCCGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474910.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330396.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OBa26759
Clone ID Related Accession (Same CDS sequence)	XM_015474910.1
Accession Version	XM_015474910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330396.1
CDS	1..4824
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474910.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474910.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474911.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330397.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OBa26760
Clone ID Related Accession (Same CDS sequence)	XM_015474911.2 , XM_015474911.1
Accession Version	XM_015474911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330397.1
CDS	1..4764
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474911.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474911.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474911.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330397.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OBa26760
Clone ID Related Accession (Same CDS sequence)	XM_015474911.2 , XM_015474911.1
Accession Version	XM_015474911.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330397.1
CDS	1..4764
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474911.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474911.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGTGATGGCC CAGGCGGCGA GGGCAGCGCC TACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGCGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TGAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTTTTCTGA AGACGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCCGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGTCTG CTGGCCACAC TCTTGCACAA CCTCATCTCG 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGCGA TCTCTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGG TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AATGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAATTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GCGAGGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCGATGG CCCACGAGAT CTGCTACTCC 
GTGTTGTGTC TCTTCTCGTA CGTGGCTGCC GTCCGCAGCA GTGAGGAGGA CCTCAGGACC 
CCGCCCCGGC CCGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474912.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330398.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

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CloneID	OBa26761
Clone ID Related Accession (Same CDS sequence)	XM_015474912.1
Accession Version	XM_015474912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG 
TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG 
CCCCGGCCCG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330398.1
CDS	1..4761
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474912.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474912.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACCGATCC AGACTCTGTC 
ATCAGTGTGA GTCCCGCTGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGT 
GATGGCCCAG GCGGCGAGGG CAGCGCCTAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGCGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTGA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT TTTCTGAAGA CGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCGATGGCCC ACGAGATCTG CTACTCCGTG 
TTGTGTCTCT TCTCGTACGT GGCTGCCGTC CGCAGCAGTG AGGAGGACCT CAGGACCCCG 
CCCCGGCCCG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975370.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831138.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OBa26762
Clone ID Related Accession (Same CDS sequence)	XM_024975370.1 , XM_015474914.1
Accession Version	XM_024975370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831138.1
CDS	1..4743
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024975370.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975370.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474914.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330400.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OBa26762
Clone ID Related Accession (Same CDS sequence)	XM_024975370.1 , XM_015474914.1
Accession Version	XM_015474914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330400.1
CDS	1..4743
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474914.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474914.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474915.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330401.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

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CloneID	OBa26763
Clone ID Related Accession (Same CDS sequence)	XM_015474915.1 , XM_015474915.2
Accession Version	XM_015474915.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330401.1
CDS	1..4740
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474915.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474915.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474915.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330401.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OBa26763
Clone ID Related Accession (Same CDS sequence)	XM_015474915.1 , XM_015474915.2
Accession Version	XM_015474915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330401.1
CDS	1..4740
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474915.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474915.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCCGGG 
AGTGGTGAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474916.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330402.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

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CloneID	OBa26764
Clone ID Related Accession (Same CDS sequence)	XM_015474916.1
Accession Version	XM_015474916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330402.1
CDS	1..4698
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474916.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474916.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024975372.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831140.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OBa26765
Clone ID Related Accession (Same CDS sequence)	XM_024975372.1 , XM_015474917.1
Accession Version	XM_024975372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831140.1
CDS	1..4683
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024975372.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975372.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474917.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330403.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OBa26765
Clone ID Related Accession (Same CDS sequence)	XM_024975372.1 , XM_015474917.1
Accession Version	XM_015474917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330403.1
CDS	1..4683
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474917.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474917.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGTCAGTGC GCCAGCGAGC CTCTGACAGC CCGTGCCTCC AGCCCCAGTG TGGCTTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GATTCAGCCA GCATGTCAGT 
GGCCATCGGG CTCAAAAGCT GCTGCGGCCC AGCAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCTCGAGC GAGCTCGCCC ACCTCCACTG TCTCCAGCAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTGGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCGA ACCTCACACT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GGCACAGGCG GGCGTTGGTG GACCAGAAGT CGTCCGTCAT TAAACACAGC 
CCGACCGTGA AGAGAGAGCC CCCGTCACCC CAGGGCCGAG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGCGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT CGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAC GAGGCCCGGC AGGATGTCAT CCCCGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGCATG TTGGATCTGC TCAAGTGCAC AGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCTGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTTCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACA 
GAGAGCATCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCAC AGCTGAGGGT CCCCCAGCTG GGACCTCTGG CACCGAGTGC CCCAGGAAAG 
AGTCCCAAAG AACTGGACAC CAGAAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATCGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAT CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCC GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGTCTGCTG GCCACACTCT TGCACAACCT CATCTCGTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGCGATCT CTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGGTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAT 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
TTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGCG AGGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474918.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330404.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

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CloneID	OBa26766
Clone ID Related Accession (Same CDS sequence)	XM_015474918.1 , XM_015474918.2
Accession Version	XM_015474918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330404.1
CDS	1..4638
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474918.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474918.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474918.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330404.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

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CloneID	OBa26766
Clone ID Related Accession (Same CDS sequence)	XM_015474918.1 , XM_015474918.2
Accession Version	XM_015474918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330404.1
CDS	1..4638
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474918.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474918.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCG ATGGCCCACG AGATCTGCTA CTCCGTGTTG 
TGTCTCTTCT CGTACGTGGC TGCCGTCCGC AGCAGTGAGG AGGACCTCAG GACCCCGCCC 
CGGCCCGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474919.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330405.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OBa26767
Clone ID Related Accession (Same CDS sequence)	XM_015474919.1
Accession Version	XM_015474919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT 
CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG 
CCCGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330405.1
CDS	1..4635
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474919.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474919.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGTGATGGC 
CCAGGCGGCG AGGGCAGCGC CTACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGCGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTGAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTTTTCTG AAGACGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCCGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGTCT GCTGGCCACA CTCTTGCACA ACCTCATCTC GTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGCG ATCTCTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG GTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAATGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAATTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGCGAGGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCGATG GCCCACGAGA TCTGCTACTC CGTGTTGTGT 
CTCTTCTCGT ACGTGGCTGC CGTCCGCAGC AGTGAGGAGG ACCTCAGGAC CCCGCCCCGG 
CCCGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474920.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330406.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OBa26768
Clone ID Related Accession (Same CDS sequence)	XM_015474920.1 , XM_015474920.2
Accession Version	XM_015474920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC 
GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG 
GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT 
GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG 
CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC 
GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT 
GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG 
CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG 
ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG 
CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG 
GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC 
CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC 
AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG 
TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330406.1
CDS	1..4626
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474920.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474920.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC 
GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG 
GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT 
GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG 
CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC 
GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT 
GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG 
CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG 
ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG 
CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG 
GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC 
CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC 
AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG 
TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474920.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330406.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

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CloneID	OBa26768
Clone ID Related Accession (Same CDS sequence)	XM_015474920.1 , XM_015474920.2
Accession Version	XM_015474920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC 
GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG 
GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT 
GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG 
CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC 
GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT 
GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG 
CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG 
ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG 
CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG 
GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC 
CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC 
AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG 
TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330406.1
CDS	1..4626
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_015474920.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474920.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGTGATGG CCCAGGCGGC 
GAGGGCAGCG CCTACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGCGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTGAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTTTTCT GAAGACGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAGTT CTGGGAAGAC 
GCGTTCTTAG ATGCCGTGAT GTTGGAGAGA GAAGGAATGG GCATGGACCA GGGTCCCCAG 
GAAATGATAG ACAGGTACCT GTCCCTGGGA GAACACGACC GGAAGCGCCT GGAGGACGAT 
GAAGACCGTC TGCTGGCCAC ACTCTTGCAC AACCTCATCT CGTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG GAAGAAGGTG AGGCGCCTGA TGGGAAAGTC CCATATTGGG 
CTTGTGTACA GTCAGCAAAT CAATGAAGTC CTTGACCAGC TGGCCAACCT GAATGGACGC 
GATCTCTCCA TCCGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT CGTGGTACAT 
GCAGGGACAG ACACGAATGG AGATATCTTT TTCATGGAGG TGTGTGACGA CTGCGTGGTG 
CTGCGCAGTA ACATCGGGAC GGTGTACGAG CGCTGGTGGT ACGAGAAACT CATCAACATG 
ACCTACTGCC CCAAGACCAA GGTGCTGTGC TTGTGGCGCA GAAATGGCTC CGAGACCCAG 
CTCAACAAGT TCTATACTAA GAAGTGTCGG GAGCTGTACT ACTGCGTGAA GGACAGCATG 
GAGCGCGCCG CCGCCCGCCA GCAGAGCATC AAGCCGGGGC CGGAATTGGG TGGCGAGTTC 
CCCGTGCAGG ACATGAAGAC TGGCGAGGGG GGCTTGCTGC AGGTCACCCT CGAAGGGATC 
AACCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCGAT GGCCCACGAG ATCTGCTACT CCGTGTTGTG TCTCTTCTCG 
TACGTGGCTG CCGTCCGCAG CAGTGAGGAG GACCTCAGGA CCCCGCCCCG GCCCGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_015474921.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330407.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

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CloneID	OBa26769
Clone ID Related Accession (Same CDS sequence)	XM_015474921.1
Accession Version	XM_015474921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330407.1
CDS	1..4440
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_015474921.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474921.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT CTACGGCAAT 
GATGTGGATT CGGTGGATGT TGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC AGGGCTTTCA GATGCACACA CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTTTAGCC TCACGCCCCC GGCAGACTCC TTTCGCCGAG 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTATG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG TGGTTCCCAA 
CTGGCCGAGG CGCTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGACGAC 
AGTGGGAGTG ACAGCATGGA TTATGACGAT TCAAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA ATGCGATATC AACGGTGATA CTCCTAACGT GGACCCTCTG 
ACGCACGCGG CACTGGGAGA TGCCAGCGAG GTGGCGATTG AGGAGCTGCA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCC GGATGGCGAG AGCTCTCAGG AAAACCCGCC CCTGCGCTCC 
AGCCCCAGCA CCACTGTGAG CAGTAGCCCC AGCACCATTA TCCACGGCGC CAGCTCTGAA 
CCTGTTGACT CAGCAGAGAC CGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCGTACCC 
ACCGTGCCTC CCGGCATGGG CAAGTGCAGC ATGGACAGGC ATCAGACAGA AACCGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAGCAGC TTGAAGCTGG CAAGTGACTC AGACGCAGAG 
TCGGACTCTC GAGCGAGCTC GCCCACCTCC ACTGTCTCCA GCAACAGCAC GGAGGGCTTC 
GGGGGCATCA TGTCTTTGGC CAGCAGCCTG TATCGGAACC ACAGCACCAG CTTCAGCCTT 
TCGAACCTCA CACTGCCCAC CAAAGGAGCC CGAGAGAAGA GCACGCCCTT CCCCAGTCTG 
AAAGGGCACA GGCGGGCGTT GGTGGACCAG AAGTCGTCCG TCATTAAACA CAGCCCGACC 
GTGAAGAGAG AGCCCCCGTC ACCCCAGGGC CGAGCCAGCA ACTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GCGTCGGCTG GCTCAACATG 
AAGAAGGTGC GGCGGCTGCT GGAGAGCGAG CAGCTGCGCG TCTTCGTCCT GAGCAAGCTG 
AACCGCACGG TGCAGTCCGA GGACGAGGCC CGGCAGGATG TCATCCCCGA CGTGGAGATC 
AGCCGGAAGG TGTACAAGGG CATGTTGGAT CTGCTCAAGT GCACAGTCCT CAGTCTGGAG 
CAGTCCTTTG CCCACGCTGG CCTGGGTGGC ATGGCCAGCA TCTTCGGGCT TCTGGAGATC 
GCCCAGACCC ACTACTACAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC CACAGAGAGC 
ATCAGTACAC CAGTCGGCAA GGATCCTGGC CTGGCTGGGC GGGGGGACCC AAAGGCCATG 
GCACAGCTGA GGGTCCCCCA GCTGGGACCT CTGGCACCGA GTGCCCCAGG AAAGAGTCCC 
AAAGAACTGG ACACCAGAAG TCTAAAGGAA GAGAACTTTG TGGCATCTAT CGGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGGTTCC CAGAGGACCG ATCCAGACTC TGTCATCAGT 
GTGAGTCCCG CTGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGTGAT 
GGCCCAGGCG GCGAGGGCAG CGCCTACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGC 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTGAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTTTT CTGAAGACGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCCGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG TCTGCTGGCC ACACTCTTGC ACAACCTCAT CTCGTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GCGATCTCTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGGTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAATGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAATTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGCGAGG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_010812836.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811138.1 Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OBa52688
Clone ID Related Accession (Same CDS sequence)	XM_010812836.2
Accession Version	XM_010812836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGTTCCTG 
AAATTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811138.1
CDS	274..5211
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHSEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPFVSEEVGPKTSESGPSLQPPSADSTPDVSQSPRARRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQYLKALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVAIEELQNQKEAEEPGPDGESSQENPPLRSSPSTTVSSSPSTIIHGASSEPVDSAETDDKAAGGVPKSVPTVPPGMGKCSMDRHQTETGEGSVRQRASDSPCLQPQCGFPPEEDDEQGESYTPRFSQHVSGHRAQKLLRPSSLKLASDSDAESDSRASSPTSTVSSNSTEGFGGIMSLASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGHRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDEARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESISTPVGKDPGLAGRGDPKAMAQLRVPQLGPLAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKNQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVISVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAYLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRFSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKKRLCREFLKLKKW

Target ORF information:

RefSeq Version	XM_010812836.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812836.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCACTA 
GAGGACCACT CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCGGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGACC CTGCGGGATG ACACCTCTTT TGTCTTCACC 
CTCACGGACA AGGACACTGG TGTCACCCGA TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GCGTGCCTAA GGAAAAGGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTTCGT CTCCGAGGAG GTGGGCCCCA AGACCTCGGA GAGCGGCCCA 
TCCCTGCAGC CGCCCAGTGC CGACTCCACC CCAGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCCGGGCCA AGGCGGGGAG CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC TACCTTCCGA GAGTGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGAATCTT TACTGGATCG TTGTTAGTGG AGGAGAAGTC AAGTGCCCTG 
CTGCACGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGGT TGCTGCGCTC CCCGGTACCT 
GTCTCGGGGC AGAAGCGAGT GGACATTGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTCCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAGCAC 
AAGGTTGTGC TCCAGTCCCG AGACTACAAT GCACTCTCCA TGTCCGTGAT GGCCTTTGTG 
GCGATGATCT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCATCAGCAG AACAGCTGCT CTTGGCTCCA ACACCATACA TCATCGGAGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGACG TGTGGCTAGT AGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACTAATGCG GAAGTGCTGC CTATCCTGCC AGAGCCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG TACCTGAAGG CCCTCGCCAG CATGAGTCTC 
AACACCCAAC CCATCCTCAA TCTGGAGAAA TTCCACGAGG GCCAGGAGAT CCCCCTTCTC 
CTGGGAAGGC CTTCTAATGA CCTGCAGTCC ACACCTTCCA CCGAGTTCAA CCCGCTCATC 
TACGGCAATG ATGTGGATTC GGTGGATGTT GCAACCAGAG TGGCCATGGT CCGTTTCTTC 
AACTCCCCCA ACGTGCTGCA GGGCTTTCAG ATGCACACAC GTACCCTACG TCTCTTCCCC 
CGGCCCGTGG TGGCTTTCCA AGCCGGCTCC TTTTTAGCCT CACGCCCCCG GCAGACTCCT 
TTCGCCGAGA AACTGGCCAG GACTCAGGCC GTGGAGTACT TTGGAGAATG GATCCTGAAC 
CCCACCAACT ACGCCTTCCA GCGGATTCAC AACAACATGT TCGATCCAGC TCTGATTGGC 
GACAAGCCCA AGTGGTATGC CCACCAGCTG CAGCCCATTC ACTATCGAGT GTATGACAGT 
GGTTCCCAAC TGGCCGAGGC GCTGAGCGTG CCGCCCGAGC GAGACTCTGA CTCTGACCCA 
ACTGACGACA GTGGGAGTGA CAGCATGGAT TATGACGATT CAAGCTCTTC TTACTCTTCC 
CTTGGTGACT TTGTCAGCGA GATGATGAAA TGCGATATCA ACGGTGATAC TCCTAACGTG 
GACCCTCTGA CGCACGCGGC ACTGGGAGAT GCCAGCGAGG TGGCGATTGA GGAGCTGCAG 
AACCAGAAGG AGGCAGAGGA ACCCGGCCCG GATGGCGAGA GCTCTCAGGA AAACCCGCCC 
CTGCGCTCCA GCCCCAGCAC CACTGTGAGC AGTAGCCCCA GCACCATTAT CCACGGCGCC 
AGCTCTGAAC CTGTTGACTC AGCAGAGACC GACGATAAGG CGGCAGGAGG CGTCCCCAAG 
TCCGTACCCA CCGTGCCTCC CGGCATGGGC AAGTGCAGCA TGGACAGGCA TCAGACAGAA 
ACCGGAGAGG GGTCAGTGCG CCAGCGAGCC TCTGACAGCC CGTGCCTCCA GCCCCAGTGT 
GGCTTTCCCC CTGAGGAAGA CGATGAGCAG GGTGAAAGTT ACACCCCCCG ATTCAGCCAG 
CATGTCAGTG GCCATCGGGC TCAAAAGCTG CTGCGGCCCA GCAGCTTGAA GCTGGCAAGT 
GACTCAGACG CAGAGTCGGA CTCTCGAGCG AGCTCGCCCA CCTCCACTGT CTCCAGCAAC 
AGCACGGAGG GCTTCGGGGG CATCATGTCT TTGGCCAGCA GCCTGTATCG GAACCACAGC 
ACCAGCTTCA GCCTTTCGAA CCTCACACTG CCCACCAAAG GAGCCCGAGA GAAGAGCACG 
CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA AATACTCTAA TGGAGATTGT TACTGAAGCC 
GGCCCCGGGA GTGGTGAAGG GCACAGGCGG GCGTTGGTGG ACCAGAAGTC GTCCGTCATT 
AAACACAGCC CGACCGTGAA GAGAGAGCCC CCGTCACCCC AGGGCCGAGC CAGCAACTCT 
AGTGAGAACC AGCAGTTCCT GAAGGAGGTG GTGCACAGCG TGCTGGACGG CCAGGGCGTC 
GGCTGGCTCA ACATGAAGAA GGTGCGGCGG CTGCTGGAGA GCGAGCAGCT GCGCGTCTTC 
GTCCTGAGCA AGCTGAACCG CACGGTGCAG TCCGAGGACG AGGCCCGGCA GGATGTCATC 
CCCGACGTGG AGATCAGCCG GAAGGTGTAC AAGGGCATGT TGGATCTGCT CAAGTGCACA 
GTCCTCAGTC TGGAGCAGTC CTTTGCCCAC GCTGGCCTGG GTGGCATGGC CAGCATCTTC 
GGGCTTCTGG AGATCGCCCA GACCCACTAC TACAGTAAAG AACCAGACAA GCGGAAGAGA 
AGTCCCACAG AGAGCATCAG TACACCAGTC GGCAAGGATC CTGGCCTGGC TGGGCGGGGG 
GACCCAAAGG CCATGGCACA GCTGAGGGTC CCCCAGCTGG GACCTCTGGC ACCGAGTGCC 
CCAGGAAAGA GTCCCAAAGA ACTGGACACC AGAAGTCTAA AGGAAGAGAA CTTTGTGGCA 
TCTATCGAAT TGTGGAGCAA GCACCAGGAA GTGAAAAATC AAAAAGCTTT GGAAAAACAG 
AGGCCTGAAG TAATCAAACC CACCTTTGAC CTTGGTGAGA CCGACGAGAA AAAGTCCCAG 
GTCAGCGCAG ACAGTGGTGT GAGCCTGACA TCTGGTTCCC AGAGGACCGA TCCAGACTCT 
GTCATCAGTG TGAGTCCCGC TGTGATGATC CGAAGCTCAA GTCAGGACTC TGAAGTTAGC 
ACCGTGGTGA GCAACAGCTC CGGAGAGACC CTGGGAGCAG ACAGCGACCT CAGCAGCAGT 
GCGGGTGATG GCCCAGGCGG CGAGGGCAGC GCCTACTTGG CAGGCTCTCG AGGCACCTTG 
TCTGACAGCG AGATCGAGAC CAACTCTGCC ACCAGTGCCA TCTTTGGTAA AGCCCATAGC 
TTGAAGCCAA GTGTGAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTTTTC TGAAGACGTC 
AGCCAGCGAG TCTACCTCTA CGAGGGACTC CTAGGAAGGG ACAAAGGATC GATGTGGGAC 
CAGTTAGAGG ACGCTGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AGTTCTGGGA AGACGCGTTC TTAGATGCCG TGATGTTGGA GAGAGAAGGA 
ATGGGCATGG ACCAGGGTCC CCAGGAAATG ATAGACAGGT ACCTGTCCCT GGGAGAACAC 
GACCGGAAGC GCCTGGAGGA CGATGAAGAC CGTCTGCTGG CCACACTCTT GCACAACCTC 
ATCTCGTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTGATGGGAA AGTCCCATAT TGGGCTTGTG TACAGTCAGC AAATCAATGA AGTCCTTGAC 
CAGCTGGCCA ACCTGAATGG ACGCGATCTC TCCATCCGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTCGTGGT ACATGCAGGG ACAGACACGA ATGGAGATAT CTTTTTCATG 
GAGGTGTGTG ACGACTGCGT GGTGCTGCGC AGTAACATCG GGACGGTGTA CGAGCGCTGG 
TGGTACGAGA AACTCATCAA CATGACCTAC TGCCCCAAGA CCAAGGTGCT GTGCTTGTGG 
CGCAGAAATG GCTCCGAGAC CCAGCTCAAC AAGTTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGCG TGAAGGACAG CATGGAGCGC GCCGCCGCCC GCCAGCAGAG CATCAAGCCG 
GGGCCGGAAT TGGGTGGCGA GTTCCCCGTG CAGGACATGA AGACTGGCGA GGGGGGCTTG 
CTGCAGGTCA CCCTCGAAGG GATCAACCTC AAGTTCATGC ACAACCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTCC GAGGCGTCTT TGTCCTGGAG 
GAATTTGGTA ATTACACTAT TTTGCTCTTA GGTCTGGACT CACATGGCAG TAACTCAAAC 
CTCGGAGCTC CAGAGGAGGG ACTAGAGACA GAGAAGAAGC GCCTCTGCAG AGAGTTCCTG 
AAATTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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オンライン注文

MADD cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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