HLCS cDNA ORF clone, Bos taurus(cattle)

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa52678 XM_015462210.1
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52678 XM_010801734.1
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52674 XM_024997049.1
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa52674 XM_024997047.1
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa16182 XM_005202127.4
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa16182 XM_015462209.1
Latest version!
Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OBa16182 XM_005202127.3 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa52678
    Clone ID Related Accession (Same CDS sequence) XM_015462210.1 , XM_010801734.1
    Accession Version XM_015462210.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
    CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA
    CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG
    GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT
    GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG
    ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT
    TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG
    CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG
    ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC
    CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG
    CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG
    GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC
    TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA
    TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT
    TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG
    GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG
    CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC
    CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC
    ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT
    CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC
    AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC
    CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG
    CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG
    CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA
    CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC
    TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA
    AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT
    CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA
    GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA
    CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA
    CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC
    ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC
    ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG
    GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG
    ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC
    GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC
    ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT
    ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC
    ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC
    GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC
    GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA
    AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC
    CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317696.1
    CDS16..2625
    Translation

    Target ORF information:

    RefSeq Version XM_015462210.1
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
    CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA
    CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG
    GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT
    GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG
    ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT
    TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG
    CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG
    ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC
    CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG
    CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG
    GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC
    TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA
    TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT
    TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG
    GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG
    CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC
    CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC
    ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT
    CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC
    AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC
    CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG
    CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG
    CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA
    CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC
    TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA
    AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT
    CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA
    GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA
    CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA
    CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC
    ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC
    ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG
    GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG
    ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC
    GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC
    ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT
    ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC
    ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC
    GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC
    GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA
    AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC
    CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52678
    Clone ID Related Accession (Same CDS sequence) XM_015462210.1 , XM_010801734.1
    Accession Version XM_010801734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
    CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA
    CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG
    GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT
    GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG
    ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT
    TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG
    CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG
    ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC
    CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG
    CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG
    GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC
    TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA
    TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT
    TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG
    GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG
    CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC
    CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC
    ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT
    CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC
    AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC
    CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG
    CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG
    CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA
    CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC
    TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA
    AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT
    CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA
    GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA
    CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA
    CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC
    ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC
    ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG
    GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG
    ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC
    GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC
    ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT
    ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC
    ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC
    GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC
    GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA
    AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC
    CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800036.1
    CDS16..2625
    Translation

    Target ORF information:

    RefSeq Version XM_010801734.1
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
    CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA
    CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG
    GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT
    GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG
    ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT
    TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG
    CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG
    ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC
    CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG
    CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG
    GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC
    TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA
    TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT
    TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG
    GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG
    CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC
    CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC
    ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT
    CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC
    AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC
    CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG
    CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG
    CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA
    CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC
    TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA
    AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT
    CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA
    GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA
    CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA
    CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC
    ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC
    ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG
    GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG
    ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC
    GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC
    ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT
    ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC
    ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC
    GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC
    GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA
    AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC
    CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52674
    Clone ID Related Accession (Same CDS sequence) XM_024997047.1 , XM_024997049.1
    Accession Version XM_024997047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
    AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA
    TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT
    TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT
    GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT
    TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA
    GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG
    AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC
    TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT
    GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG
    TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG
    TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC
    TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC
    TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC
    GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC
    AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT
    GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG
    GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG
    ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG
    CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT
    CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT
    GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA
    GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC
    ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG
    ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG
    GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC
    CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC
    GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG
    CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA
    CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC
    CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA
    CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT
    GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA
    CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC
    CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT
    CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852815.1
    CDS490..2646
    Translation

    Target ORF information:

    RefSeq Version XM_024997047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
    AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA
    TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT
    TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT
    GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT
    TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA
    GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG
    AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC
    TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT
    GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG
    TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG
    TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC
    TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC
    TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC
    GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC
    AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT
    GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG
    GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG
    ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG
    CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT
    CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT
    GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA
    GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC
    ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG
    ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG
    GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC
    CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC
    GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG
    CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA
    CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC
    CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA
    CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT
    GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA
    CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC
    CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT
    CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52674
    Clone ID Related Accession (Same CDS sequence) XM_024997047.1 , XM_024997049.1
    Accession Version XM_024997049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
    AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA
    TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT
    TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT
    GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT
    TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA
    GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG
    AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC
    TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT
    GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG
    TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG
    TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC
    TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC
    TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC
    GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC
    AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT
    GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG
    GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG
    ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG
    CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT
    CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT
    GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA
    GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC
    ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG
    ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG
    GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC
    CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC
    GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG
    CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA
    CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC
    CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA
    CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT
    GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA
    CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC
    CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT
    CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852817.1
    CDS459..2615
    Translation

    Target ORF information:

    RefSeq Version XM_024997049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
    AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA
    TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT
    TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT
    GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT
    TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA
    GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG
    AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC
    TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT
    GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG
    TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG
    TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC
    TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC
    TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC
    GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC
    AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT
    GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG
    GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG
    ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG
    CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT
    CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT
    GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA
    GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC
    ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG
    ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG
    GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC
    CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC
    GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG
    CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA
    CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC
    CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA
    CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT
    GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA
    CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC
    CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT
    CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16182
    Clone ID Related Accession (Same CDS sequence) XM_015462209.1 , XM_005202127.4 , XM_005202127.3
    Accession Version XM_015462209.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317695.1
    CDS60..2678
    Translation

    Target ORF information:

    RefSeq Version XM_015462209.1
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16182
    Clone ID Related Accession (Same CDS sequence) XM_015462209.1 , XM_005202127.4 , XM_005202127.3
    Accession Version XM_005202127.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202127.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202184.1
    CDS82..2700
    Translation

    Target ORF information:

    RefSeq Version XM_005202127.4
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202127.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16182
    Clone ID Related Accession (Same CDS sequence) XM_015462209.1 , XM_005202127.4 , XM_005202127.3
    Accession Version XM_005202127.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product biotin--protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202184.1
    CDS60..2678
    Translation

    Target ORF information:

    RefSeq Version XM_005202127.3
    Organism Bos taurus(cattle)
    Definition Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202127.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
    CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC
    GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT
    GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT
    TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG
    AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG
    CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC
    TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA
    CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG
    GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC
    CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG
    CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC
    ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC
    ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC
    AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC
    AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC
    CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC
    ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG
    CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC
    TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC
    CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA
    GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC
    GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG
    CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC
    TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA
    TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC
    TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA
    ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA
    TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA
    TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG
    ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA
    ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT
    TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG
    AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG
    GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA
    TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC
    AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC
    TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT
    GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC
    TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG
    GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC
    TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC
    CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC
    GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.