HLCS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLCS
Entrez Gene ID	534247
Full Name	holocarboxylase synthetase
General protein information	Preferred Names holocarboxylase synthetase Names biotin--protein ligase holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015462209.1	XP_015317695.1	biotin--protein ligase isoform X1
XM_005202127.4	XP_005202184.1	biotin--protein ligase isoform X1
XM_024997047.1	XP_024852815.1	biotin--protein ligase isoform X2
XM_024997049.1	XP_024852817.1	biotin--protein ligase isoform X2
XM_015462210.1	XP_015317696.1	biotin--protein ligase isoform X2
XM_005202127.3	XP_005202184.1	biotin--protein ligase isoform X1
XM_010801734.1	XP_010800036.1	biotin--protein ligase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Gene Ontology

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa52678	XM_015462210.1 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52678	XM_010801734.1 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52674	XM_024997049.1 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa52674	XM_024997047.1 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa16182	XM_005202127.4 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa16182	XM_015462209.1 Latest version!	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa16182	XM_005202127.3	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLCS ( XM_015462210.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317696.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52678
Clone ID Related Accession (Same CDS sequence)	XM_015462210.1 , XM_010801734.1
Accession Version	XM_015462210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG 
GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC 
AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC 
CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG 
CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA 
GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA 
CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA 
AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317696.1
CDS	16..2625
Translation	MAWDGGWGSRGANQRGLALSPDLLQRNLYKNPWEALPRGQPGEGLDRLPPPPPASEHLSPETSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_015462210.1
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462210.1

ORF Insert Sequence:

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG 
GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC 
AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC 
CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG 
CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA 
GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA 
CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA 
AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_010801734.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800036.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52678
Clone ID Related Accession (Same CDS sequence)	XM_015462210.1 , XM_010801734.1
Accession Version	XM_010801734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG 
GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC 
AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC 
CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG 
CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA 
GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA 
CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA 
AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800036.1
CDS	16..2625
Translation	MAWDGGWGSRGANQRGLALSPDLLQRNLYKNPWEALPRGQPGEGLDRLPPPPPASEHLSPETSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_010801734.1
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801734.1

ORF Insert Sequence:

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAA CCTCTATAAA AACCCTTGGG AAGCGCTACC GAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTATCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CTGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCT GGTTCTGTAT CCACAAGGGG GCCAGGCGGG 
GCCCCTGGGG GCAGTGAGCC AGCCATGGAC GGTGACAGAG GCGTGGGCTC CATTGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGACGATGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCATG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCTGC CCTGGCCGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CAGAAAACGC TCTCAGGGAC CCGTGGCCAG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCATGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCGGGTGACC 
AGCAAGTCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGC AGACGGGAGC 
CTGCTGAGGC TCAGCATCCT GAGCAGTGGC TGCGTTTATG AGGAGGGGCC CGGGGAGCCG 
CTCGACCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TCGGGATTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTGACGACGC TGGGCCTAAG CTGCGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCTGGGGT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAA 
GTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCATA 
CCCGTGGTGA CTGACACAGA GTCCTGCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA GCTTGGGAAA GTCATTCTGT TTGCCGAAGT AACGCCCACC 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCACTGA GATCCGCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCGGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA TGGGGCACAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACGG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CTCTCTACTA TAAGTACTGG GTACACAGCG CTCAGCAAGT CTGCCTGGGA 
AGCGCTGAGG GACCACAGGC GTGGATAGCT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAG ACGGTGATGT GGTCACGGTG CATCCCGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_024997047.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852815.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52674
Clone ID Related Accession (Same CDS sequence)	XM_024997047.1 , XM_024997049.1
Accession Version	XM_024997047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA 
TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT 
TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT 
GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG 
AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC 
TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG 
TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC 
TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC 
GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT 
GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG 
GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG 
ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT 
GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA 
GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC 
ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA 
CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA 
CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT 
GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA 
CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT 
CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852815.1
CDS	490..2646
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_024997047.1
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997047.1

ORF Insert Sequence:

ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA 
TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT 
TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT 
GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG 
AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC 
TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG 
TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC 
TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC 
GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT 
GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG 
GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG 
ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT 
GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA 
GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC 
ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA 
CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA 
CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT 
GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA 
CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT 
CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_024997049.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852817.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52674
Clone ID Related Accession (Same CDS sequence)	XM_024997047.1 , XM_024997049.1
Accession Version	XM_024997049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA 
TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT 
TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT 
GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG 
AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC 
TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG 
TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC 
TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC 
GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT 
GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG 
GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG 
ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT 
GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA 
GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC 
ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA 
CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA 
CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT 
GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA 
CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT 
CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852817.1
CDS	459..2615
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_024997049.1
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997049.1

ORF Insert Sequence:

ATGGCTAATG GACTGATACC CCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGACCTG CCAGCCCAGC TCCTGCAGCC TGACCCTGAA 
TCTTGTATTG AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCTGGT 
TCTGTATCCA CAAGGGGGCC AGGCGGGGCC CCTGGGGGCA GTGAGCCAGC CATGGACGGT 
GACAGAGGCG TGGGCTCCAT TGAGCACTGT CCCCTCCATC TGTCCAGCTG CCATGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CAGCGGTTTG GAGGGCCTCG ACGATGACCT CCGCCCCGAG 
AGGGATGAGA GGAGGGTCAA TGTCATGGGC AAGGCCCCCA ACATCCTCCT TTACGTGGGC 
TCTGACTCTC GGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCTGCCCT GGCCGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCAG AAAACGCTCT CAGGGACCCG 
TGGCCAGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCATGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCGTCGTCC 
TTCACGCTGC CTGGCTTCCG GGTGACCAGC AAGTCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGCAGA CGGGAGCCTG CTGAGGCTCA GCATCCTGAG CAGTGGCTGC 
GTTTATGAGG AGGGGCCCGG GGAGCCGCTC GACCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGATG GGGACAGGCT TGTGGTGCAA GTGCCTTTCG GGATTCACGG AGGAGAAGCT 
GTTCTTTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGTAGTGCA AACACAAGAG 
GATTTTAACC TGCTTAAATC AAGCAACTTT AGAAGATACG AAGTCCTTAC GGAGATCCTG 
ACGACGCTGG GCCTAAGCTG CGACGCAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGACGGCGA CCGAGGAAAT CTGGGGTCCT CTTGTGCAGT GGCTGGGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAAGTC TCCCTGAAAT TCATTTCATC CTACACATCT 
GAAATAGAAA TCACCCCATC CGCCATACCC GTGGTGACTG ACACAGAGTC CTGCTCATCA 
GAAAACTTTG ACTTCGAGAT TTACCGACAA AACCTGAAGA CAAAGAAGCT TGGGAAAGTC 
ATTCTGTTTG CCGAAGTAAC GCCCACCACA ATGAATCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCACTGAGAT CCGCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CGGTAAGATC CATCCCCGGG TACCAGGACA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCAACA 
CTTATGGGAG AAACATTCTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCTACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACATGG GGCACAGCTA 
CAGCCCCTGA GAGCCGACTG TCTCATCGCC CGAACGGTGA CCGTGCTGGA GAAGCTGATT 
GACGTGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCTC TCTACTATAA GTACTGGGTA 
CACAGCGCTC AGCAAGTCTG CCTGGGAAGC GCTGAGGGAC CACAGGCGTG GATAGCTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAGACG GTGATGTGGT CACGGTGCAT 
CCCGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATCG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_015462209.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317695.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16182
Clone ID Related Accession (Same CDS sequence)	XM_015462209.1 , XM_005202127.4 , XM_005202127.3
Accession Version	XM_015462209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644565.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317695.1
CDS	60..2678
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_015462209.1
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462209.1

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005202127.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202184.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16182
Clone ID Related Accession (Same CDS sequence)	XM_015462209.1 , XM_005202127.4 , XM_005202127.3
Accession Version	XM_005202127.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202127.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202184.1
CDS	82..2700
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_005202127.4
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202127.4

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005202127.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202184.1 Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16182
Clone ID Related Accession (Same CDS sequence)	XM_015462209.1 , XM_005202127.4 , XM_005202127.3
Accession Version	XM_005202127.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202184.1
CDS	60..2678
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDLPAQLLQPDPESCIERNPPEPDTMEDPQSPGSVSTRGPGGAPGGSEPAMDGDRGVGSIEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLDDDLRPERDERRVNVMGKAPNILLYVGSDSRDGQDRLQQVRSALADCVDTDCYTLYHLPPENALRDPWPDNCLLLVIATGEPVPEDLSRRFMAYLSQGGKVLALASSFTLPGFRVTSKSPLQDTVQSLVFCKADGSLLRLSILSSGCVYEEGPGEPLDPSKLHGHLDSEDGDRLVVQVPFGIHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIWGPLVQWLGKHVDPEGVIRSSKVSLKFISSYTSEIEITPSAIPVVTDTESCSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSALGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAQLQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVCLGSAEGPQAWIAGLDDSGFLLVHQEDGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_005202127.3
Organism	Bos taurus(cattle)
Definition	Bos taurus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202127.3

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTTAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTATCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC TGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCTG GTTCTGTATC CACAAGGGGG 
CCAGGCGGGG CCCCTGGGGG CAGTGAGCCA GCCATGGACG GTGACAGAGG CGTGGGCTCC 
ATTGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGACGATGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCATGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCTGCC CTGGCCGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC AGAAAACGCT CTCAGGGACC CGTGGCCAGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCATGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCGT CCTTCACGCT GCCTGGCTTC 
CGGGTGACCA GCAAGTCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGCA 
GACGGGAGCC TGCTGAGGCT CAGCATCCTG AGCAGTGGCT GCGTTTATGA GGAGGGGCCC 
GGGGAGCCGC TCGACCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT CGGGATTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TGACGACGCT GGGCCTAAGC 
TGCGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCTGGGGTC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAAG TCTCCCTGAA ATTCATTTCA TCCTACACAT CTGAAATAGA AATCACCCCA 
TCCGCCATAC CCGTGGTGAC TGACACAGAG TCCTGCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAG CTTGGGAAAG TCATTCTGTT TGCCGAAGTA 
ACGCCCACCA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCACTGAG ATCCGCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCGGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAT GGGGCACAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACGGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC TCTCTACTAT AAGTACTGGG TACACAGCGC TCAGCAAGTC 
TGCCTGGGAA GCGCTGAGGG ACCACAGGCG TGGATAGCTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAGA CGGTGATGTG GTCACGGTGC ATCCCGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HLCS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム