GEMIN5 cDNA ORF clone, Bos taurus(cattle)

The following GEMIN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEMIN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236777 XM_024995269.1
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa236774 XM_010807535.3
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236775 XM_010807536.3
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236776 XM_010807537.3
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236778 XM_010807538.3
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa52456 XM_010798518.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52456 XM_010807535.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52457 XM_010798519.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52457 XM_010807536.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52458 XM_015464341.1
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52458 XM_010807537.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52459 XM_015464342.1
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52459 XM_010807538.2
Latest version!
Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236777
    Clone ID Related Accession (Same CDS sequence) XM_024995269.1
    Accession Version XM_024995269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCAATG GGAAAGTTAT AACACAAACT CCAGTAACAA AAGGCTGCTA CTTAGCCACT 
    GGGAGCAAAG ACCAGACCAT TCGGATCTGG AGCTGCTCTA GAGGCCGAGG GGTGATGACT
    TTGAAACTGC CCTTTCTTAA AAGAAGAGGA GGGGGTGTAG ACCCAACTGT TAAAGAGCGC
    CTTTGGTTGA CACTCCATTG GCCCAAGGAT CAGCCAACTC AGCTGGTATC CAGCTGTTTT
    GGAGGTGAAC TGCTGCTATG GGATCTCACT CAGTCTTGGA GACGGAAATA TACCCTCTTT
    AGTGGTTCAT CAGAAGGGCA AAATCATTCA AGGATTGTGT TTAATTTATG TCCTCTGCAA
    ACTGAAGATG ACAAACAGCT GCTGCTTTCC ATATCAATGG ACAGAGATGT AAAATGTTGG
    GACATGGCCA CTCTGGAGTG CTGCTGGAGC CTGCCTTCCC TCGGTGGGTT TGCCTACAGT
    CTGGCTTTCT CTCCTGTGGA CACAGGCTGC TTAGCGATAG GCGTCGGGGA TGGCATGATC
    CGCGTATGGA ATACACTCTC CATAAAGAAC AACTATGATG TGAAACATTT TTGGCAAGGT
    GTCAAGTCCA AGGTTACAGC GCTGTGCTGG CACCCGACCA AGGAAGGCTG CTTAGCTTTT
    GGAACTGATG ATGGAAAAGT GGGATTGTAC GACACCTACT CCAACAAACC TCCACAGATT
    TCCAGCACGT ATCACAAGAA GACTGTGTAC TCGTTAGCCT GGGGACCACC AGTACCCGCC
    ATGGCACTAG GAGAAGGAGA CAGACCTTCC CTCACTTTAT ACAGTTGTGG AGGAGAAGGG
    ATTGTCTTAC AGCACAACCC TTGGAAGCTT AGTGGAGAGG CCTTTGACAT CAATAAACTC
    ATCAGGGACA CCAATTCAAT CAAATACAAA TTGCCTGTAC ACACAGAGAT CAGCTGGAAA
    GCAGATGGCA AAATCATGGC TCTTGGCAAT GAAGATGGAT CAATAGAAAT ATTCAAGCTT
    CCTAACTTGA AACTGATCTG CAGCATCCAG CAGCATCATA AGCTTGTGAA TACCATTAGC
    TGGCATCATG AGCATGGCAG CCAGCCAGAA CTGAGCTATC TGATGGCCTC TGGATCCAAC
    AATGCAGTCA TTTATGTGCA CAACCTGCAG ACTGTCATAG AGAGCAATCC TCAATCTCCA
    GTGACCATTA CAGAGCCCTA CCGGACCCTG TCAGGGCACA CAGCCAAGAT CACCAGTCTG
    GCATGGAGCC CACATCATGA TGGAAGGCTG GTATCTGCTT CCTATGATGG TACAGCTCAG
    GTGTGGGATA CTCTCCAGGA AGAGCCTCTG TGCAATTTCC GGGGACATCG AGGTCGACTG
    CTTTGCGTTG TGTGGTCCCC GTTGGATCCA GATTGCATCT ACTCAGGGGC AGATGATTTC
    TGTGTGTATA AATGGCTCAC TTCCATGCAG GAACACTCCC GACCTCCTCA AGGCAAGAAA
    AGTATTGAAT TAGAGAAAAA GCGGCTCTCT CAACCTAAAC CAAAGCCCAA AAAGAAGAAA
    AAGCCCACCT TGAGAGTGCC TGTAAGGCAA GAATTATGTG ATGGGAATGA AGAAGAGAGT
    GTAAAGGAGA GCCTGGGCCC TATTGAAAAT GGCGTGTCAG ACCAGGAGAC TGAGGAGGAA
    GCCCGTGAGC CAGAGCCACC CTGTGGTGTC ACCGCAGCGG TTTCTAAAGA ACCAATTATC
    TGCACTCCAG CAGCCTTAGG TTTTGAAAAG TCAAAAAGCA CCATTAATAA CAAAGTCACT
    TTACTGAAAA AGGAGGTACC TAAAGAGAAG CCAGAAGCCT TAATCAAGAA GCGAAAAGCT
    CGTTCCATGC TTCCACTGAG TACAAGTCTG GACCACAGAT CCAAAGAGGA GCTTCATCAG
    GACTGTTTGG TATTAGCCAC CGCCAAACAC TCCACAGCAG GGCCAAACGA GGATGTGTCT
    ACTGATCTTG AGGAAAGATT TCACCTGGGG CTTTTCACAG ACAGGGCTAC CCTGTACAGA
    ATGATTGAGG TTGAAGGAAA AAGTCACTTG GAAAATGGCC ACCCCGAGTT ATTTCACCAG
    CTCATGCTTT GGAAAGGAGA TCTAAAAGGT GTTCTGCAGA CTGCAGCAGA AAGAGGGGAG
    CTGACAGACA ATCTCGTGGC TATGGCACCA GTCGCCGGTT ACCATGTGTG GGTATGGGCT
    GTAGAAGCTT TTGCCAAACA ACTGTGTTTT CAGGACCAGT ATGTCAAGGC TGCCTCTCAC
    CTGCTTTCTA TCCACAAAGT GTATGAAGCT GTGGAGCTGC TCAAGTCAAA CCATTTCTAC
    AGGGAAGCTA TTGCAATTGC TAAGGCCCGG CTGCGCCCAG AGGACCCAAT CATAAAAGAC
    CTGTACCTCA GCTGGGGAGC CATCCTAGAA AAAGACGGCC ACTATGCCTT AGCAGCAAAA
    TGCTATTTAG GGGCCACTTC AGCTTATGAT GCAGCCAAAG TTCTGGCCAA AAAGGGGGAT
    GCGGCATCAC TTAGGACGGC AGCAGAGTTG GCCTCCATCG TGGGAGAGAA TGAGTTGTCA
    GCTTCCCTGG CTCTAAGATG TGCCCAAGAA CTGCTTCTGG CCCGGAACTG GGTGGGAGCT
    CAGGAGGCCC TGCAGCTCCA CACAAGTCTA AAGGGTCAGA GATTGGTGTT TTGCCTTCTT
    GAGCTACTGT CCAAGCACCT GGAGGAAAAG CAGCTCTCTG AGGGCAAAAG CTCCTTTTCT
    TCTTACAATG ATTGGCCCAC AGACAGCGAA GGGTCCTTTG TGGAGAGGGT GACTGCAGTA
    TGGAAGAGCA CCTTCGGCCT CGACTCCCCA GAGCAGTACC AGGCAGCATT TCAGCAGCTG
    CAAAATATCA AGTACCCATC TGCTACAAGT AACACTCCCT CCAAACAGCT TCTGCTCCAC
    ATTTGCCACG ACCTGACCTT GGCAGTGCTA AGCCAGGAAG CGGGCTCCTG GGACGCAGCT
    GTGGAAGGAT TGCTTCGGGC TGTGATCCGG AGCTACGACT CAGCAAACTT CACCATCATG
    CAGGAAGTGC ACTCAGCCTT TCTTCCGCAG GGCTGTGACC ATCTAAGAGA CAAACTGGGT
    GATCATCAGT CTCCCACCAC ACCAGCTTTC AAAAGTCTGA AGGCCTTTTT TATTTACGGA
    TGTCTATATG AATTCTGGTG GTCTCTCTCT GGGCCTTGCC CGGAGTCCAG TGTCTGGCTA
    AGAGCTGCTC ACAGAAGTAA AACCTCTACT GAACAGAGCC AAGAGGTTGA CAGTGTCAGC
    CCTGAAGAAA CTGACCCCAA ACCTTCCCAG CCAGAGCCAA GCAGGACTCC AGAACTAGAC
    GTGAGACTTT CAGAAGAAGG TGAACAAATG CAGAACGCTT GTAAGGAGCT TTTGTCAGAA
    AAGCATGCTG GTCTCCAAAA TGCACAGAGA ACTGTTGCTG AAGTCCAAGA GACCTTGGCA
    GAAATGATCC GCCAACACCA GAAGAGTCAA CTGTGTAAAT CCACAGCAAA TGGCCCTGAT
    AAGAATGAAC CTGAACAGGA AGGACAGTCC CTCTCTCATC CTCAGAACCA GTGTAAAGAA
    GAAAAAAATG AACCAGTTTC TTTGCCTGAA TTAACCAAAA GACTTACAGA GGCAAATGAG
    AGAATAGCTA AGCTTCCTGA GAGTATTAAG ACCTGGCCTT TCCCGGACGT GCTGGAATGC
    TGCCTCATCC TGCTTCACAT TGGGTCCCAA TGTCCTAGCT TTGTGACCCA GGAAATGCAG
    CAACAGGCTC AAGAGTTCCT TCAGAAGTAC GGCAACACAA AAGTTTACAA ACGACATTAC
    CAGACCCTCT GCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851037.1
    CDS145..4002
    Translation

    Target ORF information:

    RefSeq Version XM_024995269.1
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCAATG GGAAAGTTAT AACACAAACT CCAGTAACAA AAGGCTGCTA CTTAGCCACT 
    GGGAGCAAAG ACCAGACCAT TCGGATCTGG AGCTGCTCTA GAGGCCGAGG GGTGATGACT
    TTGAAACTGC CCTTTCTTAA AAGAAGAGGA GGGGGTGTAG ACCCAACTGT TAAAGAGCGC
    CTTTGGTTGA CACTCCATTG GCCCAAGGAT CAGCCAACTC AGCTGGTATC CAGCTGTTTT
    GGAGGTGAAC TGCTGCTATG GGATCTCACT CAGTCTTGGA GACGGAAATA TACCCTCTTT
    AGTGGTTCAT CAGAAGGGCA AAATCATTCA AGGATTGTGT TTAATTTATG TCCTCTGCAA
    ACTGAAGATG ACAAACAGCT GCTGCTTTCC ATATCAATGG ACAGAGATGT AAAATGTTGG
    GACATGGCCA CTCTGGAGTG CTGCTGGAGC CTGCCTTCCC TCGGTGGGTT TGCCTACAGT
    CTGGCTTTCT CTCCTGTGGA CACAGGCTGC TTAGCGATAG GCGTCGGGGA TGGCATGATC
    CGCGTATGGA ATACACTCTC CATAAAGAAC AACTATGATG TGAAACATTT TTGGCAAGGT
    GTCAAGTCCA AGGTTACAGC GCTGTGCTGG CACCCGACCA AGGAAGGCTG CTTAGCTTTT
    GGAACTGATG ATGGAAAAGT GGGATTGTAC GACACCTACT CCAACAAACC TCCACAGATT
    TCCAGCACGT ATCACAAGAA GACTGTGTAC TCGTTAGCCT GGGGACCACC AGTACCCGCC
    ATGGCACTAG GAGAAGGAGA CAGACCTTCC CTCACTTTAT ACAGTTGTGG AGGAGAAGGG
    ATTGTCTTAC AGCACAACCC TTGGAAGCTT AGTGGAGAGG CCTTTGACAT CAATAAACTC
    ATCAGGGACA CCAATTCAAT CAAATACAAA TTGCCTGTAC ACACAGAGAT CAGCTGGAAA
    GCAGATGGCA AAATCATGGC TCTTGGCAAT GAAGATGGAT CAATAGAAAT ATTCAAGCTT
    CCTAACTTGA AACTGATCTG CAGCATCCAG CAGCATCATA AGCTTGTGAA TACCATTAGC
    TGGCATCATG AGCATGGCAG CCAGCCAGAA CTGAGCTATC TGATGGCCTC TGGATCCAAC
    AATGCAGTCA TTTATGTGCA CAACCTGCAG ACTGTCATAG AGAGCAATCC TCAATCTCCA
    GTGACCATTA CAGAGCCCTA CCGGACCCTG TCAGGGCACA CAGCCAAGAT CACCAGTCTG
    GCATGGAGCC CACATCATGA TGGAAGGCTG GTATCTGCTT CCTATGATGG TACAGCTCAG
    GTGTGGGATA CTCTCCAGGA AGAGCCTCTG TGCAATTTCC GGGGACATCG AGGTCGACTG
    CTTTGCGTTG TGTGGTCCCC GTTGGATCCA GATTGCATCT ACTCAGGGGC AGATGATTTC
    TGTGTGTATA AATGGCTCAC TTCCATGCAG GAACACTCCC GACCTCCTCA AGGCAAGAAA
    AGTATTGAAT TAGAGAAAAA GCGGCTCTCT CAACCTAAAC CAAAGCCCAA AAAGAAGAAA
    AAGCCCACCT TGAGAGTGCC TGTAAGGCAA GAATTATGTG ATGGGAATGA AGAAGAGAGT
    GTAAAGGAGA GCCTGGGCCC TATTGAAAAT GGCGTGTCAG ACCAGGAGAC TGAGGAGGAA
    GCCCGTGAGC CAGAGCCACC CTGTGGTGTC ACCGCAGCGG TTTCTAAAGA ACCAATTATC
    TGCACTCCAG CAGCCTTAGG TTTTGAAAAG TCAAAAAGCA CCATTAATAA CAAAGTCACT
    TTACTGAAAA AGGAGGTACC TAAAGAGAAG CCAGAAGCCT TAATCAAGAA GCGAAAAGCT
    CGTTCCATGC TTCCACTGAG TACAAGTCTG GACCACAGAT CCAAAGAGGA GCTTCATCAG
    GACTGTTTGG TATTAGCCAC CGCCAAACAC TCCACAGCAG GGCCAAACGA GGATGTGTCT
    ACTGATCTTG AGGAAAGATT TCACCTGGGG CTTTTCACAG ACAGGGCTAC CCTGTACAGA
    ATGATTGAGG TTGAAGGAAA AAGTCACTTG GAAAATGGCC ACCCCGAGTT ATTTCACCAG
    CTCATGCTTT GGAAAGGAGA TCTAAAAGGT GTTCTGCAGA CTGCAGCAGA AAGAGGGGAG
    CTGACAGACA ATCTCGTGGC TATGGCACCA GTCGCCGGTT ACCATGTGTG GGTATGGGCT
    GTAGAAGCTT TTGCCAAACA ACTGTGTTTT CAGGACCAGT ATGTCAAGGC TGCCTCTCAC
    CTGCTTTCTA TCCACAAAGT GTATGAAGCT GTGGAGCTGC TCAAGTCAAA CCATTTCTAC
    AGGGAAGCTA TTGCAATTGC TAAGGCCCGG CTGCGCCCAG AGGACCCAAT CATAAAAGAC
    CTGTACCTCA GCTGGGGAGC CATCCTAGAA AAAGACGGCC ACTATGCCTT AGCAGCAAAA
    TGCTATTTAG GGGCCACTTC AGCTTATGAT GCAGCCAAAG TTCTGGCCAA AAAGGGGGAT
    GCGGCATCAC TTAGGACGGC AGCAGAGTTG GCCTCCATCG TGGGAGAGAA TGAGTTGTCA
    GCTTCCCTGG CTCTAAGATG TGCCCAAGAA CTGCTTCTGG CCCGGAACTG GGTGGGAGCT
    CAGGAGGCCC TGCAGCTCCA CACAAGTCTA AAGGGTCAGA GATTGGTGTT TTGCCTTCTT
    GAGCTACTGT CCAAGCACCT GGAGGAAAAG CAGCTCTCTG AGGGCAAAAG CTCCTTTTCT
    TCTTACAATG ATTGGCCCAC AGACAGCGAA GGGTCCTTTG TGGAGAGGGT GACTGCAGTA
    TGGAAGAGCA CCTTCGGCCT CGACTCCCCA GAGCAGTACC AGGCAGCATT TCAGCAGCTG
    CAAAATATCA AGTACCCATC TGCTACAAGT AACACTCCCT CCAAACAGCT TCTGCTCCAC
    ATTTGCCACG ACCTGACCTT GGCAGTGCTA AGCCAGGAAG CGGGCTCCTG GGACGCAGCT
    GTGGAAGGAT TGCTTCGGGC TGTGATCCGG AGCTACGACT CAGCAAACTT CACCATCATG
    CAGGAAGTGC ACTCAGCCTT TCTTCCGCAG GGCTGTGACC ATCTAAGAGA CAAACTGGGT
    GATCATCAGT CTCCCACCAC ACCAGCTTTC AAAAGTCTGA AGGCCTTTTT TATTTACGGA
    TGTCTATATG AATTCTGGTG GTCTCTCTCT GGGCCTTGCC CGGAGTCCAG TGTCTGGCTA
    AGAGCTGCTC ACAGAAGTAA AACCTCTACT GAACAGAGCC AAGAGGTTGA CAGTGTCAGC
    CCTGAAGAAA CTGACCCCAA ACCTTCCCAG CCAGAGCCAA GCAGGACTCC AGAACTAGAC
    GTGAGACTTT CAGAAGAAGG TGAACAAATG CAGAACGCTT GTAAGGAGCT TTTGTCAGAA
    AAGCATGCTG GTCTCCAAAA TGCACAGAGA ACTGTTGCTG AAGTCCAAGA GACCTTGGCA
    GAAATGATCC GCCAACACCA GAAGAGTCAA CTGTGTAAAT CCACAGCAAA TGGCCCTGAT
    AAGAATGAAC CTGAACAGGA AGGACAGTCC CTCTCTCATC CTCAGAACCA GTGTAAAGAA
    GAAAAAAATG AACCAGTTTC TTTGCCTGAA TTAACCAAAA GACTTACAGA GGCAAATGAG
    AGAATAGCTA AGCTTCCTGA GAGTATTAAG ACCTGGCCTT TCCCGGACGT GCTGGAATGC
    TGCCTCATCC TGCTTCACAT TGGGTCCCAA TGTCCTAGCT TTGTGACCCA GGAAATGCAG
    CAACAGGCTC AAGAGTTCCT TCAGAAGTAC GGCAACACAA AAGTTTACAA ACGACATTAC
    CAGACCCTCT GCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236774
    Clone ID Related Accession (Same CDS sequence) XM_010807535.3
    Accession Version XM_010807535.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCGACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGCAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805837.2
    CDS87..4616
    Translation

    Target ORF information:

    RefSeq Version XM_010807535.3
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807535.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCGACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGCAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236775
    Clone ID Related Accession (Same CDS sequence) XM_010807536.3
    Accession Version XM_010807536.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805838.2
    CDS84..4610
    Translation

    Target ORF information:

    RefSeq Version XM_010807536.3
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807536.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC CAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236776
    Clone ID Related Accession (Same CDS sequence) XM_010807537.3
    Accession Version XM_010807537.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805839.2
    CDS84..4610
    Translation

    Target ORF information:

    RefSeq Version XM_010807537.3
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807537.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC GACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGCAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236778
    Clone ID Related Accession (Same CDS sequence) XM_010807538.3
    Accession Version XM_010807538.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCGAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGC AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805840.2
    CDS84..4607
    Translation

    Target ORF information:

    RefSeq Version XM_010807538.3
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807538.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCAT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTCTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTC GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCCAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCGAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGC AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52456
    Clone ID Related Accession (Same CDS sequence) XM_010807535.2 , XM_010798518.2
    Accession Version XM_010807535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805837.1
    CDS77..4606
    Translation

    Target ORF information:

    RefSeq Version XM_010807535.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52456
    Clone ID Related Accession (Same CDS sequence) XM_010807535.2 , XM_010798518.2
    Accession Version XM_010798518.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796820.2
    CDS77..4606
    Translation

    Target ORF information:

    RefSeq Version XM_010798518.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGC AGGGCCAAAC
    GAGGATGTGT CTACTGATCT TGAGGAAAGA TTTCACCTGG GGCTTTTCAC AGACAGGGCT
    ACCCTGTACA GAATGATTGA GGTTGAAGGA AAAAGTCACT TGGAAAATGG CCACCCCGAG
    TTATTTCACC AGCTCATGCT TTGGAAAGGA GATCTAAAAG GTGTTCTGCA GACTGCAGCA
    GAAAGAGGGG AGCTGACAGA CAATCTCGTG GCTATGGCAC CAGTCGCCGG TTACCATGTG
    TGGGTATGGG CTGTAGAAGC TTTTGCCAAA CAACTGTGTT TTCAGGACCA GTATGTCAAG
    GCTGCCTCTC ACCTGCTTTC TATCCACAAA GTGTATGAAG CTGTGGAGCT GCTCAAGTCA
    AACCATTTCT ACAGGGAAGC TATTGCAATT GCTAAGGCCC GGCTGCGCCC AGAGGACCCA
    ATCATAAAAG ACCTGTACCT CAGCTGGGGA GCCATCCTAG AAAAAGACGG CCACTATGCC
    TTAGCAGCAA AATGCTATTT AGGGGCCACT TCAGCTTATG ATGCAGCCAA AGTTCTGGCC
    AAAAAGGGGG ATGCGGCATC ACTTAGGACG GCAGCAGAGT TGGCCTCCAT CGTGGGAGAG
    AATGAGTTGT CAGCTTCCCT GGCTCTAAGA TGTGCCCAAG AACTGCTTCT GGCCCGGAAC
    TGGGTGGGAG CTCAGGAGGC CCTGCAGCTC CACACAAGTC TAAAGGGTCA GAGATTGGTG
    TTTTGCCTTC TTGAGCTACT GTCCAAGCAC CTGGAGGAAA AGCAGCTCTC TGAGGGCAAA
    AGCTCCTTTT CTTCTTACAA TGATTGGCCC ACAGACAGCG AAGGGTCCTT TGTGGAGAGG
    GTGACTGCAG TATGGAAGAG CACCTTCGGC CTCAACTCCC CAGAGCAGTA CCAGGCAGCA
    TTTCAGCAGC TGCAAAATAT CAAGTACCCA TCTGCTACAA GTAACACTCC CTCCAAACAG
    CTTCTGCTCC ACATTTGCCA CGACCTGACC TTGGCAGTGC TAAGCCAGGA AGCGGGCTCC
    TGGGACGCAG CTGTGGAAGG ATTGCTTCGG GCTGTGATCC GGAGCTACGA CTCAGGAAAC
    TTCACCATCA TGCAGGAAGT GCACTCAGCC TTTCTTCCGC AGGGCTGTGA CCATCTAAGA
    GACAAACTGG GTGATCATCA GTCTCCCACC ACACCAGCTT TCAAAAGTCT GAAGGCCTTT
    TTTATTTACG GATGTCTATA TGAATTCTGG TGGTCTCTCT CTGGGCCTTG CCCGGAGTCC
    AGTGTCTGGC TAAGAGCTGC TCACAGAAGT AAAACCTCTA CTGAACAGAG CCAAGAGGTT
    GACAGTGTCA GCCCTGAAGA AACTGACCCC AAACCTTCCC AGCCAGAGCC AAGCAGGACT
    CCAGAACTAG ACGTGAGACT TTCAGAAGAA GGTGAACAAA TGCAGAACGC TTGTAAGGAG
    CTTTTGTCAG AAAAGCATGC TGGTCTCCAA AATGCACAGA GAACTGTTGC TGAAGTCCAA
    GAGACCTTGG CAGAAATGAT CCGCCAACAC CAGAAGAGTC AACTGTGTAA ATCCACAGCA
    AATGGCCCTG ATAAGAATGA ACCTGAACAG GAAGGACAGT CCCTCTCTCA TCCTCAGAAC
    CAGTGTAAAG AAGAAAAAAA TGAACCAGTT TCTTTGCCTG AATTAACCAA AAGACTTACA
    GAGGCAAATG AGAGAATAGC TAAGCTTCCT GAGAGTATTA AGACCTGGCC TTTCCCGGAC
    GTGCTGGAAT GCTGCCTCAT CCTGCTTCAC ATTGGGTCCC AATGTCCTAG CTTTGTGACC
    CAGGAAATGC AGCAACAGGC TCAAGAGTTC CTTCAGAAGT ACGGCAACAC AAAAGTTTAC
    AAACGACATT ACCAGACCCT CTGCACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52457
    Clone ID Related Accession (Same CDS sequence) XM_010798519.2 , XM_010807536.2
    Accession Version XM_010798519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796821.2
    CDS77..4603
    Translation

    Target ORF information:

    RefSeq Version XM_010798519.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52457
    Clone ID Related Accession (Same CDS sequence) XM_010798519.2 , XM_010807536.2
    Accession Version XM_010807536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805838.1
    CDS77..4603
    Translation

    Target ORF information:

    RefSeq Version XM_010807536.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAAGAACCTG AAATGCCCAA TGGGAAAGTT ATAACACAAA CTCCAGTAAC AAAAGGCTGC
    TACTTAGCCA CTGGGAGCAA AGACCAGACC ATTCGGATCT GGAGCTGCTC TAGAGGCCGA
    GGGGTGATGA CTTTGAAACT GCCCTTTCTT AAAAGAAGAG GAGGGGGTGT AGACCCAACT
    GTTAAAGAGC GCCTTTGGTT GACACTCCAT TGGCCCAAGG ATCAGCCAAC TCAGCTGGTA
    TCCAGCTGTT TTGGAGGTGA ACTGCTGCTA TGGGATCTCA CTCAGTCTTG GAGACGGAAA
    TATACCCTCT TTAGTGGTTC ATCAGAAGGG CAAAATCATT CAAGGATTGT GTTTAATTTA
    TGTCCTCTGC AAACTGAAGA TGACAAACAG CTGCTGCTTT CCATATCAAT GGACAGAGAT
    GTAAAATGTT GGGACATGGC CACTCTGGAG TGCTGCTGGA GCCTGCCTTC CCTCGGTGGG
    TTTGCCTACA GTCTGGCTTT CTCTCCTGTG GACACAGGCT GCTTAGCGAT AGGCGTCGGG
    GATGGCATGA TCCGCGTATG GAATACACTC TCCATAAAGA ACAACTATGA TGTGAAACAT
    TTTTGGCAAG GTGTCAAGTC GAAGGTTACA GCGCTGTGCT GGCACCCGAC CAAGGAAGGC
    TGCTTAGCTT TTGGAACTGA TGATGGAAAA GTGGGATTGT ACGACACCTA CTCCAACAAA
    CCTCCACAGA TTTCCAGCAC GTATCACAAG AAGACTGTGT ACTCGTTAGC CTGGGGACCA
    CCAGTACCCG CCATGGCACT AGGAGAAGGA GACAGACCTT CCCTCACTTT ATACAGTTGT
    GGAGGAGAAG GGATTGTCTT ACAGCACAAC CCTTGGAAGC TTAGTGGAGA GGCCTTTGAC
    ATCAATAAAC TCATCAGGGA CACCAATTCA ATCAAATACA AATTGCCTGT ACACACAGAG
    ATCAGCTGGA AAGCAGATGG CAAAATCATG GCTCTTGGCA ATGAAGATGG ATCAATAGAA
    ATATTCAAGC TTCCTAACTT GAAACTGATC TGCAGCATCC AGCAGCATCA TAAGCTTGTG
    AATACCATTA GCTGGCATCA TGAGCATGGC AGCCAGCCAG AACTGAGCTA TCTGATGGCC
    TCTGGATCCA ACAATGCAGT CATTTATGTG CACAACCTGC AGACTGTCAT AGAGAGCAAT
    CCTCAATCTC CAGTGACCAT TACAGAGCCC TACCGGACCC TGTCAGGGCA CACAGCCAAG
    ATCACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTCCTATGAT
    GGTACAGCTC AGGTGTGGGA TACTCTCCAG GAAGAGCCTC TGTGCAATTT CCGGGGACAT
    CGAGGTCGAC TGCTTTGCGT TGTGTGGTCC CCGTTGGATC CAGATTGCAT CTACTCAGGG
    GCAGATGATT TCTGTGTGTA TAAATGGCTC ACTTCCATGC AGGAACACTC CCGACCTCCT
    CAAGGCAAGA AAAGTATTGA ATTAGAGAAA AAGCGGCTCT CTCAACCTAA ACCAAAGCCC
    AAAAAGAAGA AAAAGCCCAC CTTGAGAGTG CCTGTAAGGC AAGAATTATG TGATGGGAAT
    GAAGAAGAGA GTGTAAAGGA GAGCCTGGGC CCTATTGAAA ATGGCGTGTC AGACCAGGAG
    ACTGAGGAGG AAGCCCGTGA GCCAGAGCCA CCCTGTGGTG TCACCGCAGC GGTTTCTAAA
    GAACCAATTA TCTGCACTCC AGCAGCCTTA GGTTTTGAAA AGTCAAAAAG CACCATTAAT
    AACAAAGTCA CTTTACTGAA AAAGGAGGTA CCTAAAGAGA AGCCAGAAGC CTTAATCAAG
    AAGCGAAAAG CTCGTTCCAT GCTTCCACTG AGTACAAGTC TGGACCACAG ATCCAAAGAG
    GAGCTTCATC AGGACTGTTT GGTATTAGCC ACCGCCAAAC ACTCCACAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52458
    Clone ID Related Accession (Same CDS sequence) XM_015464341.1 , XM_010807537.2
    Accession Version XM_015464341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319827.1
    CDS77..4603
    Translation

    Target ORF information:

    RefSeq Version XM_015464341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52458
    Clone ID Related Accession (Same CDS sequence) XM_015464341.1 , XM_010807537.2
    Accession Version XM_010807537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805839.1
    CDS77..4603
    Translation

    Target ORF information:

    RefSeq Version XM_010807537.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGCAGG GCCAAACGAG
    GATGTGTCTA CTGATCTTGA GGAAAGATTT CACCTGGGGC TTTTCACAGA CAGGGCTACC
    CTGTACAGAA TGATTGAGGT TGAAGGAAAA AGTCACTTGG AAAATGGCCA CCCCGAGTTA
    TTTCACCAGC TCATGCTTTG GAAAGGAGAT CTAAAAGGTG TTCTGCAGAC TGCAGCAGAA
    AGAGGGGAGC TGACAGACAA TCTCGTGGCT ATGGCACCAG TCGCCGGTTA CCATGTGTGG
    GTATGGGCTG TAGAAGCTTT TGCCAAACAA CTGTGTTTTC AGGACCAGTA TGTCAAGGCT
    GCCTCTCACC TGCTTTCTAT CCACAAAGTG TATGAAGCTG TGGAGCTGCT CAAGTCAAAC
    CATTTCTACA GGGAAGCTAT TGCAATTGCT AAGGCCCGGC TGCGCCCAGA GGACCCAATC
    ATAAAAGACC TGTACCTCAG CTGGGGAGCC ATCCTAGAAA AAGACGGCCA CTATGCCTTA
    GCAGCAAAAT GCTATTTAGG GGCCACTTCA GCTTATGATG CAGCCAAAGT TCTGGCCAAA
    AAGGGGGATG CGGCATCACT TAGGACGGCA GCAGAGTTGG CCTCCATCGT GGGAGAGAAT
    GAGTTGTCAG CTTCCCTGGC TCTAAGATGT GCCCAAGAAC TGCTTCTGGC CCGGAACTGG
    GTGGGAGCTC AGGAGGCCCT GCAGCTCCAC ACAAGTCTAA AGGGTCAGAG ATTGGTGTTT
    TGCCTTCTTG AGCTACTGTC CAAGCACCTG GAGGAAAAGC AGCTCTCTGA GGGCAAAAGC
    TCCTTTTCTT CTTACAATGA TTGGCCCACA GACAGCGAAG GGTCCTTTGT GGAGAGGGTG
    ACTGCAGTAT GGAAGAGCAC CTTCGGCCTC AACTCCCCAG AGCAGTACCA GGCAGCATTT
    CAGCAGCTGC AAAATATCAA GTACCCATCT GCTACAAGTA ACACTCCCTC CAAACAGCTT
    CTGCTCCACA TTTGCCACGA CCTGACCTTG GCAGTGCTAA GCCAGGAAGC GGGCTCCTGG
    GACGCAGCTG TGGAAGGATT GCTTCGGGCT GTGATCCGGA GCTACGACTC AGGAAACTTC
    ACCATCATGC AGGAAGTGCA CTCAGCCTTT CTTCCGCAGG GCTGTGACCA TCTAAGAGAC
    AAACTGGGTG ATCATCAGTC TCCCACCACA CCAGCTTTCA AAAGTCTGAA GGCCTTTTTT
    ATTTACGGAT GTCTATATGA ATTCTGGTGG TCTCTCTCTG GGCCTTGCCC GGAGTCCAGT
    GTCTGGCTAA GAGCTGCTCA CAGAAGTAAA ACCTCTACTG AACAGAGCCA AGAGGTTGAC
    AGTGTCAGCC CTGAAGAAAC TGACCCCAAA CCTTCCCAGC CAGAGCCAAG CAGGACTCCA
    GAACTAGACG TGAGACTTTC AGAAGAAGGT GAACAAATGC AGAACGCTTG TAAGGAGCTT
    TTGTCAGAAA AGCATGCTGG TCTCCAAAAT GCACAGAGAA CTGTTGCTGA AGTCCAAGAG
    ACCTTGGCAG AAATGATCCG CCAACACCAG AAGAGTCAAC TGTGTAAATC CACAGCAAAT
    GGCCCTGATA AGAATGAACC TGAACAGGAA GGACAGTCCC TCTCTCATCC TCAGAACCAG
    TGTAAAGAAG AAAAAAATGA ACCAGTTTCT TTGCCTGAAT TAACCAAAAG ACTTACAGAG
    GCAAATGAGA GAATAGCTAA GCTTCCTGAG AGTATTAAGA CCTGGCCTTT CCCGGACGTG
    CTGGAATGCT GCCTCATCCT GCTTCACATT GGGTCCCAAT GTCCTAGCTT TGTGACCCAG
    GAAATGCAGC AACAGGCTCA AGAGTTCCTT CAGAAGTACG GCAACACAAA AGTTTACAAA
    CGACATTACC AGACCCTCTG CACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52459
    Clone ID Related Accession (Same CDS sequence) XM_015464342.1 , XM_010807538.2
    Accession Version XM_015464342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319828.1
    CDS77..4600
    Translation

    Target ORF information:

    RefSeq Version XM_015464342.1
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52459
    Clone ID Related Accession (Same CDS sequence) XM_015464342.1 , XM_010807538.2
    Accession Version XM_010807538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product gem-associated protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805840.1
    CDS77..4600
    Translation

    Target ORF information:

    RefSeq Version XM_010807538.2
    Organism Bos taurus(cattle)
    Definition Bos taurus gem nuclear organelle associated protein 5 (GEMIN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGACAGG AACCGCGCAC GTTGCCGCCC TCCCCTAACT GGTACTGCTC CCGATGCAGC 
    GATGCCGTGC CCGGGGGCCT CTTCGGCTTC GCGGCGCGGA CTTCGGTCTT CCTTGTCCGC
    GTGGGCCCGG GCGCGGACGC GATCCCAGGG ACGCCCCCAT TTCGAGTCGT AGGCGAGTTG
    GTGGGACACA CGGAAAAGGT GTCTGGCTTC ACGTTTTGTC ATCACCCGGG TCAATACAAC
    CTCTGCGCCA CCAGCTCCGA TGATGGGACT GTGAAAATCT GGGATGTAGG GACAAAAACA
    GTTGTGACGG AACATGCACT CCATCAGCAT ACAATATCAG CATTGCATTG GTCTCCTCGA
    GTAAAGGATC TGATAGTATC TGGAGATGAA AAAGGAGTAG TTTTCTGTTA CTGGCTTAAC
    AGAAATGACA GCCAGCACCT CTTTATAGAA CCTAGGACAA TTTTCTGTCT TACTTGCTCG
    CCTCATCATG AAGATTTAGT AGCCATTGGC TACAAGGATG GCATAGTGGT TATAATTGAC
    ATCAGCAAGA AAGGAGAAGT TATGCACAGG CTTCGAGGCC ACGATGATGA GATCCATTCC
    ATAGCCTGGT GTCCCCTGTT GGGTGAAGAC TGTTTATCCA TAAATCAAGA GGAAAATTCA
    GAACCTGAAA TGCCCAATGG GAAAGTTATA ACACAAACTC CAGTAACAAA AGGCTGCTAC
    TTAGCCACTG GGAGCAAAGA CCAGACCATT CGGATCTGGA GCTGCTCTAG AGGCCGAGGG
    GTGATGACTT TGAAACTGCC CTTTCTTAAA AGAAGAGGAG GGGGTGTAGA CCCAACTGTT
    AAAGAGCGCC TTTGGTTGAC ACTCCATTGG CCCAAGGATC AGCCAACTCA GCTGGTATCC
    AGCTGTTTTG GAGGTGAACT GCTGCTATGG GATCTCACTC AGTCTTGGAG ACGGAAATAT
    ACCCTCTTTA GTGGTTCATC AGAAGGGCAA AATCATTCAA GGATTGTGTT TAATTTATGT
    CCTCTGCAAA CTGAAGATGA CAAACAGCTG CTGCTTTCCA TATCAATGGA CAGAGATGTA
    AAATGTTGGG ACATGGCCAC TCTGGAGTGC TGCTGGAGCC TGCCTTCCCT CGGTGGGTTT
    GCCTACAGTC TGGCTTTCTC TCCTGTGGAC ACAGGCTGCT TAGCGATAGG CGTCGGGGAT
    GGCATGATCC GCGTATGGAA TACACTCTCC ATAAAGAACA ACTATGATGT GAAACATTTT
    TGGCAAGGTG TCAAGTCGAA GGTTACAGCG CTGTGCTGGC ACCCGACCAA GGAAGGCTGC
    TTAGCTTTTG GAACTGATGA TGGAAAAGTG GGATTGTACG ACACCTACTC CAACAAACCT
    CCACAGATTT CCAGCACGTA TCACAAGAAG ACTGTGTACT CGTTAGCCTG GGGACCACCA
    GTACCCGCCA TGGCACTAGG AGAAGGAGAC AGACCTTCCC TCACTTTATA CAGTTGTGGA
    GGAGAAGGGA TTGTCTTACA GCACAACCCT TGGAAGCTTA GTGGAGAGGC CTTTGACATC
    AATAAACTCA TCAGGGACAC CAATTCAATC AAATACAAAT TGCCTGTACA CACAGAGATC
    AGCTGGAAAG CAGATGGCAA AATCATGGCT CTTGGCAATG AAGATGGATC AATAGAAATA
    TTCAAGCTTC CTAACTTGAA ACTGATCTGC AGCATCCAGC AGCATCATAA GCTTGTGAAT
    ACCATTAGCT GGCATCATGA GCATGGCAGC CAGCCAGAAC TGAGCTATCT GATGGCCTCT
    GGATCCAACA ATGCAGTCAT TTATGTGCAC AACCTGCAGA CTGTCATAGA GAGCAATCCT
    CAATCTCCAG TGACCATTAC AGAGCCCTAC CGGACCCTGT CAGGGCACAC AGCCAAGATC
    ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTC CTATGATGGT
    ACAGCTCAGG TGTGGGATAC TCTCCAGGAA GAGCCTCTGT GCAATTTCCG GGGACATCGA
    GGTCGACTGC TTTGCGTTGT GTGGTCCCCG TTGGATCCAG ATTGCATCTA CTCAGGGGCA
    GATGATTTCT GTGTGTATAA ATGGCTCACT TCCATGCAGG AACACTCCCG ACCTCCTCAA
    GGCAAGAAAA GTATTGAATT AGAGAAAAAG CGGCTCTCTC AACCTAAACC AAAGCCCAAA
    AAGAAGAAAA AGCCCACCTT GAGAGTGCCT GTAAGGCAAG AATTATGTGA TGGGAATGAA
    GAAGAGAGTG TAAAGGAGAG CCTGGGCCCT ATTGAAAATG GCGTGTCAGA CCAGGAGACT
    GAGGAGGAAG CCCGTGAGCC AGAGCCACCC TGTGGTGTCA CCGCAGCGGT TTCTAAAGAA
    CCAATTATCT GCACTCCAGC AGCCTTAGGT TTTGAAAAGT CAAAAAGCAC CATTAATAAC
    AAAGTCACTT TACTGAAAAA GGAGGTACCT AAAGAGAAGC CAGAAGCCTT AATCAAGAAG
    CGAAAAGCTC GTTCCATGCT TCCACTGAGT ACAAGTCTGG ACCACAGATC CAAAGAGGAG
    CTTCATCAGG ACTGTTTGGT ATTAGCCACC GCCAAACACT CCACAGGGCC AAACGAGGAT
    GTGTCTACTG ATCTTGAGGA AAGATTTCAC CTGGGGCTTT TCACAGACAG GGCTACCCTG
    TACAGAATGA TTGAGGTTGA AGGAAAAAGT CACTTGGAAA ATGGCCACCC CGAGTTATTT
    CACCAGCTCA TGCTTTGGAA AGGAGATCTA AAAGGTGTTC TGCAGACTGC AGCAGAAAGA
    GGGGAGCTGA CAGACAATCT CGTGGCTATG GCACCAGTCG CCGGTTACCA TGTGTGGGTA
    TGGGCTGTAG AAGCTTTTGC CAAACAACTG TGTTTTCAGG ACCAGTATGT CAAGGCTGCC
    TCTCACCTGC TTTCTATCCA CAAAGTGTAT GAAGCTGTGG AGCTGCTCAA GTCAAACCAT
    TTCTACAGGG AAGCTATTGC AATTGCTAAG GCCCGGCTGC GCCCAGAGGA CCCAATCATA
    AAAGACCTGT ACCTCAGCTG GGGAGCCATC CTAGAAAAAG ACGGCCACTA TGCCTTAGCA
    GCAAAATGCT ATTTAGGGGC CACTTCAGCT TATGATGCAG CCAAAGTTCT GGCCAAAAAG
    GGGGATGCGG CATCACTTAG GACGGCAGCA GAGTTGGCCT CCATCGTGGG AGAGAATGAG
    TTGTCAGCTT CCCTGGCTCT AAGATGTGCC CAAGAACTGC TTCTGGCCCG GAACTGGGTG
    GGAGCTCAGG AGGCCCTGCA GCTCCACACA AGTCTAAAGG GTCAGAGATT GGTGTTTTGC
    CTTCTTGAGC TACTGTCCAA GCACCTGGAG GAAAAGCAGC TCTCTGAGGG CAAAAGCTCC
    TTTTCTTCTT ACAATGATTG GCCCACAGAC AGCGAAGGGT CCTTTGTGGA GAGGGTGACT
    GCAGTATGGA AGAGCACCTT CGGCCTCAAC TCCCCAGAGC AGTACCAGGC AGCATTTCAG
    CAGCTGCAAA ATATCAAGTA CCCATCTGCT ACAAGTAACA CTCCCTCCAA ACAGCTTCTG
    CTCCACATTT GCCACGACCT GACCTTGGCA GTGCTAAGCC AGGAAGCGGG CTCCTGGGAC
    GCAGCTGTGG AAGGATTGCT TCGGGCTGTG ATCCGGAGCT ACGACTCAGG AAACTTCACC
    ATCATGCAGG AAGTGCACTC AGCCTTTCTT CCGCAGGGCT GTGACCATCT AAGAGACAAA
    CTGGGTGATC ATCAGTCTCC CACCACACCA GCTTTCAAAA GTCTGAAGGC CTTTTTTATT
    TACGGATGTC TATATGAATT CTGGTGGTCT CTCTCTGGGC CTTGCCCGGA GTCCAGTGTC
    TGGCTAAGAG CTGCTCACAG AAGTAAAACC TCTACTGAAC AGAGCCAAGA GGTTGACAGT
    GTCAGCCCTG AAGAAACTGA CCCCAAACCT TCCCAGCCAG AGCCAAGCAG GACTCCAGAA
    CTAGACGTGA GACTTTCAGA AGAAGGTGAA CAAATGCAGA ACGCTTGTAA GGAGCTTTTG
    TCAGAAAAGC ATGCTGGTCT CCAAAATGCA CAGAGAACTG TTGCTGAAGT CCAAGAGACC
    TTGGCAGAAA TGATCCGCCA ACACCAGAAG AGTCAACTGT GTAAATCCAC AGCAAATGGC
    CCTGATAAGA ATGAACCTGA ACAGGAAGGA CAGTCCCTCT CTCATCCTCA GAACCAGTGT
    AAAGAAGAAA AAAATGAACC AGTTTCTTTG CCTGAATTAA CCAAAAGACT TACAGAGGCA
    AATGAGAGAA TAGCTAAGCT TCCTGAGAGT ATTAAGACCT GGCCTTTCCC GGACGTGCTG
    GAATGCTGCC TCATCCTGCT TCACATTGGG TCCCAATGTC CTAGCTTTGT GACCCAGGAA
    ATGCAGCAAC AGGCTCAAGA GTTCCTTCAG AAGTACGGCA ACACAAAAGT TTACAAACGA
    CATTACCAGA CCCTCTGCAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.