SCN9A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN9A
Entrez Gene ID	533065
Full Name	sodium voltage-gated channel alpha subunit 9
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 9 Names sodium channel protein type 9 subunit alpha sodium channel, voltage-gated, type IX, alpha subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110787.2	NP_001104257.2	sodium channel protein type 9 subunit alpha
NM_001110787.3	NP_001104257.3	sodium channel protein type 9 subunit alpha
XM_024975629.1	XP_024831397.1	sodium channel protein type 9 subunit alpha isoform X3
XM_005202453.4	XP_005202510.2	sodium channel protein type 9 subunit alpha isoform X1
XM_024975627.1	XP_024831395.1	sodium channel protein type 9 subunit alpha isoform X2
XM_024975630.1	XP_024831398.1	sodium channel protein type 9 subunit alpha isoform X4
NM_001110787.1	NP_001104257.1	sodium channel protein type 9 subunit alpha
XM_005202453.3	XP_005202510.1	sodium channel protein type 9 subunit alpha isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Scn9a	NP_061340.2
Gallus gallus (chicken)	SCN9A	XP_004942840.1
	SCN9A	XP_001101662.2
Rattus norvegicus (Norway rat)	Scn9a	NP_579823.1
Homo sapiens (human)	SCN9A	NP_002968.1
Xenopus tropicalis (tropical clawed frog)	scn2a	XP_002939316.2
Pan troglodytes (chimpanzee)	SCN9A	XP_003309333.2
Canis lupus familiaris (dog)	SCN9A	XP_005640317.1
Bos taurus (cattle)	SCN9A	NP_001104257.1

Gene Ontology
Bibliography

Related articles in PubMed

Up-regulation of NaV1.7 sodium channels expression by tumor necrosis factor-? in cultured bovine adrenal chromaffin cells and rat dorsal root ganglion neurons.
Tamura R, Nemoto T, Maruta T, Onizuka S, Yanagita T, Wada A, Murakami M, Tsuneyoshi I
Anesthesia and analgesia118(2)318-24(2014 Feb)

Endothelin-1-induced down-regulation of NaV1.7 expression in adrenal chromaffin cells: attenuation of catecholamine secretion and tau dephosphorylation.
Nemoto T, Yanagita T, Maruta T, Sugita C, Satoh S, Kanai T, Wada A, Murakami M
FEBS letters587(7)898-905(2013 Apr)

Nav1.7-Ca2+ influx-induced increased phosphorylations of extracellular signal-regulated kinase (ERK) and p38 attenuate tau phosphorylation via glycogen synthase kinase-3beta: priming of Nav1.7 gating by ERK and p38.
Nemoto T, Miyazaki S, Kanai T, Maruta T, Satoh S, Yoshikawa N, Yanagita T, Wada A
European journal of pharmacology640(1-3)20-8(2010 Aug)

Nav1.7 sodium channel-induced Ca2+ influx decreases tau phosphorylation via glycogen synthase kinase-3beta in adrenal chromaffin cells.
Kanai T, Nemoto T, Yanagita T, Maruta T, Satoh S, Yoshikawa N, Wada A
Neurochemistry international54(8)497-505(2009 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TNF-alpha up-regulates NaV1.7 mRNA in both adrenal chromaffin cells and dorsal root ganglia (DRG) neurons, highlighting the peripheral nociceptive mechanism of TNF-alpha
Title: Up-regulation of NaV1.7 sodium channels expression by tumor necrosis factor-? in cultured bovine adrenal chromaffin cells and rat dorsal root ganglion neurons.

Findings suggest that the endothelin-1-induced down-regulation of NaV1.7 (SCN9A) diminishes NaV1.7-related catecholamine secretion and dephosphorylation of tau.
Title: Endothelin-1-induced down-regulation of NaV1.7 expression in adrenal chromaffin cells: attenuation of catecholamine secretion and tau dephosphorylation.

Nav1.7-Ca2+ influx-induced increased phosphorylations of extracellular signal-regulated kinase (ERK) and p38 attenuate tau phosphorylation via glycogen synthase kinase-3beta: priming of Nav1.7 gating by ERK and p38
Title: Nav1.7-Ca2+ influx-induced increased phosphorylations of extracellular signal-regulated kinase (ERK) and p38 attenuate tau phosphorylation via glycogen synthase kinase-3beta: priming of Nav1.7 gating by ERK and p38.

Na influx via scn9a converged on inhibitory phosphorylation of glycogen synthase kinase-3beta, decreasing tau phosphorylation
Title: Nav1.7 sodium channel-induced Ca2+ influx decreases tau phosphorylation via glycogen synthase kinase-3beta in adrenal chromaffin cells.

constitutively phosphorylated/activated ERK destabilizes Na(+) channel alpha-subunit mRNA via translational event, which negatively regulates steady-state level of alpha-subunit mRNA and cell surface expression of functional Na(+) channels.
Title: Destabilization of Na(v)1.7 sodium channel alpha-subunit mRNA by constitutive phosphorylation of extracellular signal-regulated kinase: negative regulation of steady-state level of cell surface functional sodium channels in adrenal chromaffin cells.

The following SCN9A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN9A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa216279	NM_001110787.2 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa238881	NM_001110787.3 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227402	XM_005202453.4 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227403	XM_024975627.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227404	XM_024975629.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227405	XM_024975630.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01359	NM_001110787.1 Latest version!	Bos taurus sodium channel, voltage-gated, type IX, alpha subunit (SCN9A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16422	XM_005202453.3 Latest version!	Bos taurus sodium channel, voltage gated, type IX alpha subunit (SCN9A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN9A ( NM_001110787.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001104257.2 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.

Price & Availability↑

CloneID	OBa216279
Clone ID Related Accession (Same CDS sequence)	NM_001110787.2
Accession Version	NM_001110787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02004356.1, DAAA02004357.1 and DAAA02004358.1. On Jun 30, 2017 this sequence version replaced NM_001110787.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822095, SAMN04019650 [ECO:0000350] ##Evidence-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CTCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTGAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCACTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAATTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104257.2
CDS	1..5964
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGINYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	NM_001110787.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110787.2

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CTCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTGAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCACTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAATTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( NM_001110787.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001104257.3 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.

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CloneID	OBa238881
Clone ID Related Accession (Same CDS sequence)	NM_001110787.3
Accession Version	NM_001110787.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000002.1. On May 29, 2018 this sequence version replaced NM_001110787.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145448, SAMN06299470 [ECO:0000350] ##Evidence-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104257.3
CDS	1..5964
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	NM_001110787.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110787.3

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( XM_005202453.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202510.2 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X1, mRNA.

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CloneID	OBa227402
Clone ID Related Accession (Same CDS sequence)	XM_005202453.4
Accession Version	XM_005202453.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202453.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202510.2
CDS	327..6290
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVDKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	XM_005202453.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202453.4

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( XM_024975627.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831395.1 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X2, mRNA.

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CloneID	OBa227403
Clone ID Related Accession (Same CDS sequence)	XM_024975627.1
Accession Version	XM_024975627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGG TACAACCAAT CAAATACACA AGAAAAGACG TCACAGTTCT 
TACCTCCTTT CTGAGGATAT GTTGAATGAC CCCAATCTCA GACAAAGGGC AATGAGTAGG 
GCAAGCATAT TAACAAACAC TGTAGAAGAA CTTGAGGAGT CCAGACAGAA ATGTCCACCT 
TGGTGGTACA GATTTGCACA CACATTCTTG ATCTGGAATT GCTCTCCATA TTGGATAAAA 
TTCAAAAAAT TTATCTATTT TATTGTAATG GATCCTTTTG TAGATCTTGC TATTACCATT 
TGCATAGTTT TAAACACATT GTTCATGGCT ATGGAACATC ACCCAATGAC TGAGGAATTC 
AAAAATGTGC TCGTTGTGGG AAATTTGGTC TTTACTGGCA TCTTTGCAGC TGAAATGGTT 
TTAAAACTAA TTGCCATGGA TCCATATGAG TATTTCCAAA TAGGATGGAA TATTTTTGAT 
AGTCTTATTG TGACCTTAAG CTTAGTGGAA CTTTTTCTAT CAGATGTGGA AGGATTGTCA 
GTTCTACGAT CATTCAGACT GCTCCGAGTT TTCAAGTTAG CAAAATCTTG GCCAACACTG 
AACATGCTGA TCAAGATCAT TGGCAATTCA GTGGGGGCTC TGGGAAACCT AACCTTGGTG 
TTGGCCATCA TCGTCTTCAT TTTTGCTGTG GTTGGCATGC AGCTCTTTGG TAAGAGCTAC 
AAAGAATGTG TTTGCAAGAT CAATGAAGAC TGCACACTCC CACGCTGGCA CATGAATGAC 
TTCTTCCACT CCTTCCTGAT TGTGTTCCGG GTGCTGTGTG GAGAGTGGAT AGAGACCATG 
TGGGACTGCA TGGAGGTCGC CGGTCAAGCC ATGTGCCTTA TTGTTTACAT GATGGTCATG 
GTCATTGGAA ACCTGGTGGT CCTCAACCTA TTTCTTGCTT TATTACTGAG CTCATTTAGT 
TCAGACAATC TTACAGCCAT TGAAGAAGAC ACTGATGCAA ACAACCTCCA GATTGCAGTG 
GCCAGAATTA AGAAGGGAAT AAAGTACGTG AAACAAACCT TACGGGAGTT TGTTCTAAAA 
GCATTTTCCA AAAAGCCAAA GATTTCCAAA GAGATTAGAC AAACAGAAGA TCTAAATTGT 
AAAAAGGAAA ACTACATCTC TAACCGCACA CTTGCTGAAA TGAGCAAGGA TCACAAATTC 
CACAAAGAAA AAGATAAAAC CAGTGGTTTT GGAAACAGCA TGGACAAATA CTTAATGGAA 
GAAAGTGATG GTCAGTCATT TATTCATAAT CCCAGCCTCA CAGTGACAGT ACCAATTGCA 
CCTGGGGAAT CTGATTTGGA AATTATGAAC ACTGAGGAAC TTAGCAGTGA TTCAGATAGC 
GAATACAGCA AAGGGAGGCT GAACCAATCA AGTTCCTCAG AGTGCAGCAC AGTTGATAAC 
CCTGTGCCTG GAGAAGGAGA AGAGGCAGAG GCTGAGCCTG TAAATTCAGA TGAGCCAGAG 
GCCTGTTTTA CCGATGGTTG CGTACAGAGG TTCCCATGCT GCCAAGTTAA CATAGAATCA 
GGGAAAGGAA AAATCTGGTG GAACATCAGG AAAACGTGCT TCAGGATAGT TGAGCACAGT 
TGGTTTGAAA GCTTCATTGT TCTCATGATC TTGCTCAGCA GTGGTGCTCT GGCTTTTGAA 
GATATATATA TTGAAAAGAA AAAGAACATT AAGATTATCC TGGAGTATGC TGACAAGATA 
TTCACGTATA TCTTCATTCT AGAAATGCTT CTAAAATGGG TAGCATATGG TTACAAAACC 
TATTTCACCA ATGCCTGGTG TTGGCTGGAT TTCTTAATTG TCGATGTTTC TTTGGTTACA 
TTAGTTGCAA ACACTCTTGG CTACTCAGAT CTTGGTCCCA TTAAATCCCT TCGGACACTT 
AGAGCTTTAA GACCTCTGCG AGCTTTATCT AGATTTGAAG GAATGAGGGT GGTTGTGAAT 
GCACTCATCG GTGCAATTCC TTCTATCATG AATGTGCTAC TTGTGTGTCT CATATTCTGG 
CTGATATTTA GCATCATGGG AGTAAATTTG TTTGCTGGCA AGTTTTATGA GTGTATTAAC 
ACCACAAATG GATTACGTTT TCCTACAAGT GAAGTGGAAA ACCGTTCTGC CTGTCTTGCG 
CTTATGAATG TCAGTCAAAA TGTGCGATGG AAAAACCTAA AAGTGAACTT TGATAATGTT 
GGACTTGGTT ACCTGTCTCT ACTTCAAGTG GCAACATTTA AGGGATGGAT GGATATTATG 
TATGCAGCAG TTGATTCTGT TAACGTAGAC AAGCAGCCCA TTTATGAATA CAGCCTCTAC 
ATGTATATTT ATTTTGTCAT CTTCATCATC TTTGGGTCAT TCTTCACTTT GAACTTGTTC 
ATTGGTGTCA TCATAGATAA TTTCAACCAA CAGAAAAAGA AGCTTGGAGG TCAAGATATC 
TTTATGACAG AAGAACAGAA GAAATATTAT AATGCAATGA AAAAGCTGGG ATCCAAGAAA 
CCACAAAAGC CAATACCTCG GCCAGGGAAC AAATTCCAAG GATGTATCTT TGACCTAGTA 
ACAAACCAAG CTTTTGATAT AGCCATCATG GTTCTGATCT GCCTCAACAT GGTAACCATG 
ATGGTGGAAA AAGAGGGACA AAGTGACTAC GTGACTGAAG TTTTAAATTG GATCAATGTG 
GTTTTTATTA TCCTGTTCTC TGGAGAATGC GTGCTGAAAC TGATCTCCCT CAGATGCTAC 
TACTTCACTG TAGGCTGGAA CATCTTTGAT TTTGTGGTTG TAATTCTCTC CATTGTAGGT 
ATGTTTCTGG CAGATTTGAT AGAAAGGTAT TTTGTGTCCC CTACCCTATT CCGAGTGATC 
CGTCTTGCCA GGATTGGCCG AATCCTGCGT CTGATCAAAG GGGCCAAGGG GATCCGCACG 
CTGCTCTTTG CTTTGATGAT GTCCCTTCCT GCGCTGTTTA ACATCGGCCT CCTGCTCTTC 
CTGGTCATGT TCATCTATGC CATCTTTGGA ATGTCCAATT TTGCCTATGT TAAAAAGGAA 
GCTGGAATTA ATGACATGTT CAACTTTGAG ACCTTCGCCA ACAGCATGAT CTGCCTGTTC 
CAGATTACCA CCTCTGCTGG CTGGGATGGA TTGCTGGCAC CTATCCTCAA TAGTAAACCA 
CCGGACTGTG ACCCCAAAAA AGTTCATCCT GGAAGCTCAG TTGAAGGAGA CTGTGGGAAC 
CCATCTGTCG GGATATTCTA TTTTGTCAGT TACATCATCA TATCATTTCT GGTCGTGGTG 
AACATGTACA TTGCTGTCAT CCTGGAGAAC TTCAGTGTTG CTACTGAAGA AAGTACTGAG 
CCCCTGAGTG AGGACGATTT TGAGATGTTC TATGAGGTTT GGGAGAAGTT TGATCCAGAC 
GCGACCCAAT TTATAGAATA TAGCAAACTC TCTGATTTTG CAGCTGCCCT GGATCCTCCT 
CTTCTCATAG CAAAACCGAA CAAAGTCCAG CTCATTGCCA TGGACCTGCC CATGGTCAGT 
GGGGACAGGA TCCACTGCCT CGACATCTTA TTTGCTTTTA CAAAGCGTGT TTTGGGGGAA 
GGTGGAGAGA TGGACTCTCT TCGTTCACAG ATGGAAGAAA GGTTCATGTC TGCAAATCCC 
TCTAAGGTGT CTTATGAGCC CATCACAACT ACACTAAAAA GAAAGCAAGA GGATGTGTCT 
GCTACAGTCA TTCAGCGTGC TTACAGACGT TACCGCCTAC GGCAAAATGT CAAAAATATA 
TCAAGTATAT ACATCAAAGA TGGTGACAGA GATGATGATT TGCCCAATAA AGAAGATATG 
GTTTTTGATA ATGTTAATGA GAACTCAAGT CCAGAAAAAA CAGGTGCAAC TCCATCCACT 
GTCTCTCCAC CTTCTTATGA TAGTGTAACA AAACCAGACA GAGAGAAATA TGAAAAAGAC 
AAAACAGAAA AGGAAGACAA AGGGAAAGAT GGCAAGGAAG GCAAAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831395.1
CDS	327..6257
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVDKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	XM_024975627.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975627.1

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGG TACAACCAAT CAAATACACA AGAAAAGACG TCACAGTTCT 
TACCTCCTTT CTGAGGATAT GTTGAATGAC CCCAATCTCA GACAAAGGGC AATGAGTAGG 
GCAAGCATAT TAACAAACAC TGTAGAAGAA CTTGAGGAGT CCAGACAGAA ATGTCCACCT 
TGGTGGTACA GATTTGCACA CACATTCTTG ATCTGGAATT GCTCTCCATA TTGGATAAAA 
TTCAAAAAAT TTATCTATTT TATTGTAATG GATCCTTTTG TAGATCTTGC TATTACCATT 
TGCATAGTTT TAAACACATT GTTCATGGCT ATGGAACATC ACCCAATGAC TGAGGAATTC 
AAAAATGTGC TCGTTGTGGG AAATTTGGTC TTTACTGGCA TCTTTGCAGC TGAAATGGTT 
TTAAAACTAA TTGCCATGGA TCCATATGAG TATTTCCAAA TAGGATGGAA TATTTTTGAT 
AGTCTTATTG TGACCTTAAG CTTAGTGGAA CTTTTTCTAT CAGATGTGGA AGGATTGTCA 
GTTCTACGAT CATTCAGACT GCTCCGAGTT TTCAAGTTAG CAAAATCTTG GCCAACACTG 
AACATGCTGA TCAAGATCAT TGGCAATTCA GTGGGGGCTC TGGGAAACCT AACCTTGGTG 
TTGGCCATCA TCGTCTTCAT TTTTGCTGTG GTTGGCATGC AGCTCTTTGG TAAGAGCTAC 
AAAGAATGTG TTTGCAAGAT CAATGAAGAC TGCACACTCC CACGCTGGCA CATGAATGAC 
TTCTTCCACT CCTTCCTGAT TGTGTTCCGG GTGCTGTGTG GAGAGTGGAT AGAGACCATG 
TGGGACTGCA TGGAGGTCGC CGGTCAAGCC ATGTGCCTTA TTGTTTACAT GATGGTCATG 
GTCATTGGAA ACCTGGTGGT CCTCAACCTA TTTCTTGCTT TATTACTGAG CTCATTTAGT 
TCAGACAATC TTACAGCCAT TGAAGAAGAC ACTGATGCAA ACAACCTCCA GATTGCAGTG 
GCCAGAATTA AGAAGGGAAT AAAGTACGTG AAACAAACCT TACGGGAGTT TGTTCTAAAA 
GCATTTTCCA AAAAGCCAAA GATTTCCAAA GAGATTAGAC AAACAGAAGA TCTAAATTGT 
AAAAAGGAAA ACTACATCTC TAACCGCACA CTTGCTGAAA TGAGCAAGGA TCACAAATTC 
CACAAAGAAA AAGATAAAAC CAGTGGTTTT GGAAACAGCA TGGACAAATA CTTAATGGAA 
GAAAGTGATG GTCAGTCATT TATTCATAAT CCCAGCCTCA CAGTGACAGT ACCAATTGCA 
CCTGGGGAAT CTGATTTGGA AATTATGAAC ACTGAGGAAC TTAGCAGTGA TTCAGATAGC 
GAATACAGCA AAGGGAGGCT GAACCAATCA AGTTCCTCAG AGTGCAGCAC AGTTGATAAC 
CCTGTGCCTG GAGAAGGAGA AGAGGCAGAG GCTGAGCCTG TAAATTCAGA TGAGCCAGAG 
GCCTGTTTTA CCGATGGTTG CGTACAGAGG TTCCCATGCT GCCAAGTTAA CATAGAATCA 
GGGAAAGGAA AAATCTGGTG GAACATCAGG AAAACGTGCT TCAGGATAGT TGAGCACAGT 
TGGTTTGAAA GCTTCATTGT TCTCATGATC TTGCTCAGCA GTGGTGCTCT GGCTTTTGAA 
GATATATATA TTGAAAAGAA AAAGAACATT AAGATTATCC TGGAGTATGC TGACAAGATA 
TTCACGTATA TCTTCATTCT AGAAATGCTT CTAAAATGGG TAGCATATGG TTACAAAACC 
TATTTCACCA ATGCCTGGTG TTGGCTGGAT TTCTTAATTG TCGATGTTTC TTTGGTTACA 
TTAGTTGCAA ACACTCTTGG CTACTCAGAT CTTGGTCCCA TTAAATCCCT TCGGACACTT 
AGAGCTTTAA GACCTCTGCG AGCTTTATCT AGATTTGAAG GAATGAGGGT GGTTGTGAAT 
GCACTCATCG GTGCAATTCC TTCTATCATG AATGTGCTAC TTGTGTGTCT CATATTCTGG 
CTGATATTTA GCATCATGGG AGTAAATTTG TTTGCTGGCA AGTTTTATGA GTGTATTAAC 
ACCACAAATG GATTACGTTT TCCTACAAGT GAAGTGGAAA ACCGTTCTGC CTGTCTTGCG 
CTTATGAATG TCAGTCAAAA TGTGCGATGG AAAAACCTAA AAGTGAACTT TGATAATGTT 
GGACTTGGTT ACCTGTCTCT ACTTCAAGTG GCAACATTTA AGGGATGGAT GGATATTATG 
TATGCAGCAG TTGATTCTGT TAACGTAGAC AAGCAGCCCA TTTATGAATA CAGCCTCTAC 
ATGTATATTT ATTTTGTCAT CTTCATCATC TTTGGGTCAT TCTTCACTTT GAACTTGTTC 
ATTGGTGTCA TCATAGATAA TTTCAACCAA CAGAAAAAGA AGCTTGGAGG TCAAGATATC 
TTTATGACAG AAGAACAGAA GAAATATTAT AATGCAATGA AAAAGCTGGG ATCCAAGAAA 
CCACAAAAGC CAATACCTCG GCCAGGGAAC AAATTCCAAG GATGTATCTT TGACCTAGTA 
ACAAACCAAG CTTTTGATAT AGCCATCATG GTTCTGATCT GCCTCAACAT GGTAACCATG 
ATGGTGGAAA AAGAGGGACA AAGTGACTAC GTGACTGAAG TTTTAAATTG GATCAATGTG 
GTTTTTATTA TCCTGTTCTC TGGAGAATGC GTGCTGAAAC TGATCTCCCT CAGATGCTAC 
TACTTCACTG TAGGCTGGAA CATCTTTGAT TTTGTGGTTG TAATTCTCTC CATTGTAGGT 
ATGTTTCTGG CAGATTTGAT AGAAAGGTAT TTTGTGTCCC CTACCCTATT CCGAGTGATC 
CGTCTTGCCA GGATTGGCCG AATCCTGCGT CTGATCAAAG GGGCCAAGGG GATCCGCACG 
CTGCTCTTTG CTTTGATGAT GTCCCTTCCT GCGCTGTTTA ACATCGGCCT CCTGCTCTTC 
CTGGTCATGT TCATCTATGC CATCTTTGGA ATGTCCAATT TTGCCTATGT TAAAAAGGAA 
GCTGGAATTA ATGACATGTT CAACTTTGAG ACCTTCGCCA ACAGCATGAT CTGCCTGTTC 
CAGATTACCA CCTCTGCTGG CTGGGATGGA TTGCTGGCAC CTATCCTCAA TAGTAAACCA 
CCGGACTGTG ACCCCAAAAA AGTTCATCCT GGAAGCTCAG TTGAAGGAGA CTGTGGGAAC 
CCATCTGTCG GGATATTCTA TTTTGTCAGT TACATCATCA TATCATTTCT GGTCGTGGTG 
AACATGTACA TTGCTGTCAT CCTGGAGAAC TTCAGTGTTG CTACTGAAGA AAGTACTGAG 
CCCCTGAGTG AGGACGATTT TGAGATGTTC TATGAGGTTT GGGAGAAGTT TGATCCAGAC 
GCGACCCAAT TTATAGAATA TAGCAAACTC TCTGATTTTG CAGCTGCCCT GGATCCTCCT 
CTTCTCATAG CAAAACCGAA CAAAGTCCAG CTCATTGCCA TGGACCTGCC CATGGTCAGT 
GGGGACAGGA TCCACTGCCT CGACATCTTA TTTGCTTTTA CAAAGCGTGT TTTGGGGGAA 
GGTGGAGAGA TGGACTCTCT TCGTTCACAG ATGGAAGAAA GGTTCATGTC TGCAAATCCC 
TCTAAGGTGT CTTATGAGCC CATCACAACT ACACTAAAAA GAAAGCAAGA GGATGTGTCT 
GCTACAGTCA TTCAGCGTGC TTACAGACGT TACCGCCTAC GGCAAAATGT CAAAAATATA 
TCAAGTATAT ACATCAAAGA TGGTGACAGA GATGATGATT TGCCCAATAA AGAAGATATG 
GTTTTTGATA ATGTTAATGA GAACTCAAGT CCAGAAAAAA CAGGTGCAAC TCCATCCACT 
GTCTCTCCAC CTTCTTATGA TAGTGTAACA AAACCAGACA GAGAGAAATA TGAAAAAGAC 
AAAACAGAAA AGGAAGACAA AGGGAAAGAT GGCAAGGAAG GCAAAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( XM_024975629.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831397.1 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X3, mRNA.

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CloneID	OBa227404
Clone ID Related Accession (Same CDS sequence)	XM_024975629.1
Accession Version	XM_024975629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGTTTCT TTGGTTACAT TAGTTGCAAA CACTCTTGGC 
TACTCAGATC TTGGTCCCAT TAAATCCCTT CGGACACTTA GAGCTTTAAG ACCTCTGCGA 
GCTTTATCTA GATTTGAAGG AATGAGGGTG GTTGTGAATG CACTCATCGG TGCAATTCCT 
TCTATCATGA ATGTGCTACT TGTGTGTCTC ATATTCTGGC TGATATTTAG CATCATGGGA 
GTAAATTTGT TTGCTGGCAA GTTTTATGAG TGTATTAACA CCACAAATGG ATTACGTTTT 
CCTACAAGTG AAGTGGAAAA CCGTTCTGCC TGTCTTGCGC TTATGAATGT CAGTCAAAAT 
GTGCGATGGA AAAACCTAAA AGTGAACTTT GATAATGTTG GACTTGGTTA CCTGTCTCTA 
CTTCAAGTGG CAACATTTAA GGGATGGATG GATATTATGT ATGCAGCAGT TGATTCTGTT 
AACGTAGACA AGCAGCCCAT TTATGAATAC AGCCTCTACA TGTATATTTA TTTTGTCATC 
TTCATCATCT TTGGGTCATT CTTCACTTTG AACTTGTTCA TTGGTGTCAT CATAGATAAT 
TTCAACCAAC AGAAAAAGAA GCTTGGAGGT CAAGATATCT TTATGACAGA AGAACAGAAG 
AAATATTATA ATGCAATGAA AAAGCTGGGA TCCAAGAAAC CACAAAAGCC AATACCTCGG 
CCAGGGAACA AATTCCAAGG ATGTATCTTT GACCTAGTAA CAAACCAAGC TTTTGATATA 
GCCATCATGG TTCTGATCTG CCTCAACATG GTAACCATGA TGGTGGAAAA AGAGGGACAA 
AGTGACTACG TGACTGAAGT TTTAAATTGG ATCAATGTGG TTTTTATTAT CCTGTTCTCT 
GGAGAATGCG TGCTGAAACT GATCTCCCTC AGATGCTACT ACTTCACTGT AGGCTGGAAC 
ATCTTTGATT TTGTGGTTGT AATTCTCTCC ATTGTAGGTA TGTTTCTGGC AGATTTGATA 
GAAAGGTATT TTGTGTCCCC TACCCTATTC CGAGTGATCC GTCTTGCCAG GATTGGCCGA 
ATCCTGCGTC TGATCAAAGG GGCCAAGGGG ATCCGCACGC TGCTCTTTGC TTTGATGATG 
TCCCTTCCTG CGCTGTTTAA CATCGGCCTC CTGCTCTTCC TGGTCATGTT CATCTATGCC 
ATCTTTGGAA TGTCCAATTT TGCCTATGTT AAAAAGGAAG CTGGAATTAA TGACATGTTC 
AACTTTGAGA CCTTCGCCAA CAGCATGATC TGCCTGTTCC AGATTACCAC CTCTGCTGGC 
TGGGATGGAT TGCTGGCACC TATCCTCAAT AGTAAACCAC CGGACTGTGA CCCCAAAAAA 
GTTCATCCTG GAAGCTCAGT TGAAGGAGAC TGTGGGAACC CATCTGTCGG GATATTCTAT 
TTTGTCAGTT ACATCATCAT ATCATTTCTG GTCGTGGTGA ACATGTACAT TGCTGTCATC 
CTGGAGAACT TCAGTGTTGC TACTGAAGAA AGTACTGAGC CCCTGAGTGA GGACGATTTT 
GAGATGTTCT ATGAGGTTTG GGAGAAGTTT GATCCAGACG CGACCCAATT TATAGAATAT 
AGCAAACTCT CTGATTTTGC AGCTGCCCTG GATCCTCCTC TTCTCATAGC AAAACCGAAC 
AAAGTCCAGC TCATTGCCAT GGACCTGCCC ATGGTCAGTG GGGACAGGAT CCACTGCCTC 
GACATCTTAT TTGCTTTTAC AAAGCGTGTT TTGGGGGAAG GTGGAGAGAT GGACTCTCTT 
CGTTCACAGA TGGAAGAAAG GTTCATGTCT GCAAATCCCT CTAAGGTGTC TTATGAGCCC 
ATCACAACTA CACTAAAAAG AAAGCAAGAG GATGTGTCTG CTACAGTCAT TCAGCGTGCT 
TACAGACGTT ACCGCCTACG GCAAAATGTC AAAAATATAT CAAGTATATA CATCAAAGAT 
GGTGACAGAG ATGATGATTT GCCCAATAAA GAAGATATGG TTTTTGATAA TGTTAATGAG 
AACTCAAGTC CAGAAAAAAC AGGTGCAACT CCATCCACTG TCTCTCCACC TTCTTATGAT 
AGTGTAACAA AACCAGACAG AGAGAAATAT GAAAAAGACA AAACAGAAAA GGAAGACAAA 
GGGAAAGATG GCAAGGAAGG CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831397.1
CDS	327..6116
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVDKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	XM_024975629.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975629.1

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGTTTCT TTGGTTACAT TAGTTGCAAA CACTCTTGGC 
TACTCAGATC TTGGTCCCAT TAAATCCCTT CGGACACTTA GAGCTTTAAG ACCTCTGCGA 
GCTTTATCTA GATTTGAAGG AATGAGGGTG GTTGTGAATG CACTCATCGG TGCAATTCCT 
TCTATCATGA ATGTGCTACT TGTGTGTCTC ATATTCTGGC TGATATTTAG CATCATGGGA 
GTAAATTTGT TTGCTGGCAA GTTTTATGAG TGTATTAACA CCACAAATGG ATTACGTTTT 
CCTACAAGTG AAGTGGAAAA CCGTTCTGCC TGTCTTGCGC TTATGAATGT CAGTCAAAAT 
GTGCGATGGA AAAACCTAAA AGTGAACTTT GATAATGTTG GACTTGGTTA CCTGTCTCTA 
CTTCAAGTGG CAACATTTAA GGGATGGATG GATATTATGT ATGCAGCAGT TGATTCTGTT 
AACGTAGACA AGCAGCCCAT TTATGAATAC AGCCTCTACA TGTATATTTA TTTTGTCATC 
TTCATCATCT TTGGGTCATT CTTCACTTTG AACTTGTTCA TTGGTGTCAT CATAGATAAT 
TTCAACCAAC AGAAAAAGAA GCTTGGAGGT CAAGATATCT TTATGACAGA AGAACAGAAG 
AAATATTATA ATGCAATGAA AAAGCTGGGA TCCAAGAAAC CACAAAAGCC AATACCTCGG 
CCAGGGAACA AATTCCAAGG ATGTATCTTT GACCTAGTAA CAAACCAAGC TTTTGATATA 
GCCATCATGG TTCTGATCTG CCTCAACATG GTAACCATGA TGGTGGAAAA AGAGGGACAA 
AGTGACTACG TGACTGAAGT TTTAAATTGG ATCAATGTGG TTTTTATTAT CCTGTTCTCT 
GGAGAATGCG TGCTGAAACT GATCTCCCTC AGATGCTACT ACTTCACTGT AGGCTGGAAC 
ATCTTTGATT TTGTGGTTGT AATTCTCTCC ATTGTAGGTA TGTTTCTGGC AGATTTGATA 
GAAAGGTATT TTGTGTCCCC TACCCTATTC CGAGTGATCC GTCTTGCCAG GATTGGCCGA 
ATCCTGCGTC TGATCAAAGG GGCCAAGGGG ATCCGCACGC TGCTCTTTGC TTTGATGATG 
TCCCTTCCTG CGCTGTTTAA CATCGGCCTC CTGCTCTTCC TGGTCATGTT CATCTATGCC 
ATCTTTGGAA TGTCCAATTT TGCCTATGTT AAAAAGGAAG CTGGAATTAA TGACATGTTC 
AACTTTGAGA CCTTCGCCAA CAGCATGATC TGCCTGTTCC AGATTACCAC CTCTGCTGGC 
TGGGATGGAT TGCTGGCACC TATCCTCAAT AGTAAACCAC CGGACTGTGA CCCCAAAAAA 
GTTCATCCTG GAAGCTCAGT TGAAGGAGAC TGTGGGAACC CATCTGTCGG GATATTCTAT 
TTTGTCAGTT ACATCATCAT ATCATTTCTG GTCGTGGTGA ACATGTACAT TGCTGTCATC 
CTGGAGAACT TCAGTGTTGC TACTGAAGAA AGTACTGAGC CCCTGAGTGA GGACGATTTT 
GAGATGTTCT ATGAGGTTTG GGAGAAGTTT GATCCAGACG CGACCCAATT TATAGAATAT 
AGCAAACTCT CTGATTTTGC AGCTGCCCTG GATCCTCCTC TTCTCATAGC AAAACCGAAC 
AAAGTCCAGC TCATTGCCAT GGACCTGCCC ATGGTCAGTG GGGACAGGAT CCACTGCCTC 
GACATCTTAT TTGCTTTTAC AAAGCGTGTT TTGGGGGAAG GTGGAGAGAT GGACTCTCTT 
CGTTCACAGA TGGAAGAAAG GTTCATGTCT GCAAATCCCT CTAAGGTGTC TTATGAGCCC 
ATCACAACTA CACTAAAAAG AAAGCAAGAG GATGTGTCTG CTACAGTCAT TCAGCGTGCT 
TACAGACGTT ACCGCCTACG GCAAAATGTC AAAAATATAT CAAGTATATA CATCAAAGAT 
GGTGACAGAG ATGATGATTT GCCCAATAAA GAAGATATGG TTTTTGATAA TGTTAATGAG 
AACTCAAGTC CAGAAAAAAC AGGTGCAACT CCATCCACTG TCTCTCCACC TTCTTATGAT 
AGTGTAACAA AACCAGACAG AGAGAAATAT GAAAAAGACA AAACAGAAAA GGAAGACAAA 
GGGAAAGATG GCAAGGAAGG CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( XM_024975630.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831398.1 Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X4, mRNA.

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CloneID	OBa227405
Clone ID Related Accession (Same CDS sequence)	XM_024975630.1
Accession Version	XM_024975630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCATAT 
GTGACAGAGT TTGTGGACCT GGGCAATGTC TCAGCGTTGA GAACCTTCAG AGTTCTCCGA 
GCTTTGAAAA CAATTTCAGT CATTCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831398.1
CDS	328..6291
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVDKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	XM_024975630.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 9 (SCN9A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975630.1

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCATAT 
GTGACAGAGT TTGTGGACCT GGGCAATGTC TCAGCGTTGA GAACCTTCAG AGTTCTCCGA 
GCTTTGAAAA CAATTTCAGT CATTCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATT AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( NM_001110787.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001104257.1 Bos taurus sodium channel, voltage-gated, type IX, alpha subunit (SCN9A), mRNA.

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CloneID	OBa01359
Clone ID Related Accession (Same CDS sequence)	NM_001110787.1
Accession Version	NM_001110787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	sodium channel protein type 9 subunit alpha
Comment	INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03051314.1, AAFC03051310.1, AAFC03051313.1 and AAFC03061554.1. On Nov 20, 2007 this sequence version replaced XM_612339.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728 [ECO:0000350] ##Evidence-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104257.1
CDS	1..5964
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGIKYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	NM_001110787.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus sodium channel, voltage-gated, type IX, alpha subunit (SCN9A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110787.1

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CCCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTAAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TCGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCGCTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAAGTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
AACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN9A ( XM_005202453.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202510.1 Bos taurus sodium channel, voltage gated, type IX alpha subunit (SCN9A), transcript variant X1, mRNA.

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CloneID	OBa16422
Clone ID Related Accession (Same CDS sequence)	XM_005202453.3
Accession Version	XM_005202453.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 9 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CTCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTGAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCACTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAATTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202510.1
CDS	325..6288
Translation	MAMLPPPGPQSFVFFTKQSLALIEQRIAEGKAKEPKEEKKDDDDEGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHAAFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFIVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCVQSSLANNETMENILNTLDEEEYAKYFYYLEGSKDALLCGFSSDSGQCPEGYTCKKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEARQKELEFQQMLDRLKKEQEEAEAIALAAAEYTSIGRSRIMGLSESSSETSKLSSKSAKERRNRRRKKNQKKLSSGEEKGDDEKLSKSESEESIRRKSFHLGVEGHRRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRHSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLVVGNLVFTGIFAAEMVLKLIAMDPYEYFQIGWNIFDSLIVTLSLVELFLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDTDANNLQIAVARIKKGINYVKQTLREFVLKAFSKKPKISKEIRQTEDLNCKKENYISNRTLAEMSKDHKFHKEKDKTSGFGNSMDKYLMEESDGQSFIHNPSLTVTVPIAPGESDLEIMNTEELSSDSDSEYSKGRLNQSSSSECSTVDNPVPGEGEEAEAEPVNSDEPEACFTDGCVQRFPCCQVNIESGKGKIWWNIRKTCFRIVEHSWFESFIVLMILLSSGALAFEDIYIEKKKNIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTNGLRFPTSEVENRSACLALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVDKQPIYEYSLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDIAIMVLICLNMVTMMVEKEGQSDYVTEVLNWINVVFIILFSGECVLKLISLRCYYFTVGWNIFDFVVVILSIVGMFLADLIERYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFETFANSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDMVFDNVNENSSPEKTGATPSTVSPPSYDSVTKPDREKYEKDKTEKEDKGKDGKEGKK

Target ORF information:

RefSeq Version	XM_005202453.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IX alpha subunit (SCN9A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202453.3

ORF Insert Sequence:

ATGGCAATGT TGCCTCCCCC GGGACCTCAG AGCTTTGTCT TCTTCACAAA ACAGTCTCTT 
GCCCTCATTG AACAACGTAT TGCTGAAGGA AAAGCAAAGG AACCCAAAGA AGAAAAGAAA 
GACGATGACG ATGAAGGGCC GAAGCCAAGC AGTGACTTGG AAGCCGGCAA GCAGCTGCCC 
TTCATCTATG GGGACATTCC TCCGGGCATG GTGTCAGAGC CTCTGGAGGA CTTGGACCCT 
TACTATGCAG ACAAAAAAAC TTTCATAGTG TTGAACAAAG GGAAAGCAAT CTTCCGTTTC 
AATGCCACAC CTGCTTTGTA CATGCTCTCT CCTTTCAGTC CTCTCAGAAG AATATCTATT 
AAGATTTTAG TACATGCCGC ATTCAGCATG CTTATCATGT GCACTATTCT GACGAACTGC 
ATATTTATGA CCATGAGTAA TCCTCCAGAC TGGACCAAGA ATGTAGAGTA CACTTTTACT 
GGAATATATA CTTTTGAGTC ACTTGTGAAA ATCCTTGCAA GAGGATTCTG TGTAGGAGAA 
TTCACTTTTC TTCGTGACCC ATGGAACTGG CTTGATTTCA TAGTCATTGT CTTTGCGTAT 
TTAACAGAAT TTGTAAACCT AGGCAATGTT TCAGCTCTTC GAACTTTCAG AGTGTTGAGA 
GCTTTGAAAA CTATTTCTGT AATCCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG 
TCTGTGAAAA AGCTCTCAGA TGTCATGATC CTGACTGTGT TCTGTCTGAG CGTGTTTGCC 
CTGATTGGGC TGCAGCTCTT TATGGGCAAC CTGAAGCATA AATGCGTGCA GAGTTCACTT 
GCAAATAATG AAACAATGGA AAACATATTG AACACCCTCG ATGAAGAAGA ATATGCAAAA 
TATTTTTATT ACTTGGAGGG ATCCAAAGAT GCTCTCCTTT GTGGTTTCAG CAGTGATTCA 
GGTCAGTGTC CAGAAGGGTA CACCTGTAAG AAGATTGGCA GAAATCCTGA CTATGGCTAC 
ACGAGCTTCG ATACTTTCAG CTGGGCCTTC TTAGCCTTGT TTCGGCTAAT GACCCAGGAT 
TACTGGGAAA ACCTTTACCA ACAGACCCTG CGTGCTGCTG GGAAAACCTA CATGATCTTC 
TTTGTTGTGG TGATTTTCCT GGGATCCTTT TATCTAATAA ACTTGATCCT GGCTGTTGTT 
GCCATGGCCT ATGAAGAACA GAACCAGGCA AACATTGAAG AAGCGAGGCA GAAAGAGTTA 
GAATTTCAGC AGATGCTGGA TCGACTTAAA AAAGAGCAAG AGGAAGCGGA GGCAATAGCA 
TTAGCAGCAG CTGAATATAC CAGCATTGGG AGAAGCAGAA TAATGGGTCT GTCTGAGAGT 
TCTTCTGAAA CATCCAAACT GAGCTCTAAA AGTGCTAAAG AAAGAAGAAA CAGGAGAAGG 
AAAAAGAACC AAAAGAAACT CTCCAGTGGG GAAGAAAAGG GAGATGATGA GAAACTGTCC 
AAATCGGAAT CCGAGGAGAG CATCAGGAGA AAAAGTTTCC ACCTTGGTGT TGAAGGTCAT 
AGGCGAGCAC GTGAAAAAAG ATTGTCTACC CCTAACCAGT CACCACTGAG CATCCGTGGC 
TCCTTGTTTT CTGCAAGGCG GAGCAGCAGA ACAAGTCTTT TCAGCTTCAA AGGTCGAGGA 
AGAGATATTG GATCTGAGAC TGAATTTGCC GACGACGAGC ACAGCATCTT TGGTGACAAC 
GAGAGCAGAA GGGGCTCCCT GTTTGTGCCC CACAGACCTC GGGAGCGGCG CAGCAGTAAC 
ATCAGCCAAG CCAGTAGGTC TCCCCCAGTG CTGCCAGTGA ACGGGAAGAT GCACAGCGCT 
GTCGACTGCA ATGGCGTGGT CTCCTTGGTG GATGGACCCT CCGCCCTCAT GCTACCCAAT 
GGACAGCTCC TGCCAGAGGT GATAATAGAT AAGGCAACTT CTGATGACAG CGGTACAACC 
AATCAAATAC ACAAGAAAAG ACGTCACAGT TCTTACCTCC TTTCTGAGGA TATGTTGAAT 
GACCCCAATC TCAGACAAAG GGCAATGAGT AGGGCAAGCA TATTAACAAA CACTGTAGAA 
GAACTTGAGG AGTCCAGACA GAAATGTCCA CCTTGGTGGT ACAGATTTGC ACACACATTC 
TTGATCTGGA ATTGCTCTCC ATATTGGATA AAATTCAAAA AATTTATCTA TTTTATTGTA 
ATGGATCCTT TTGTAGATCT TGCTATTACC ATTTGCATAG TTTTAAACAC ATTGTTCATG 
GCTATGGAAC ATCACCCAAT GACTGAGGAA TTCAAAAATG TGCTCGTTGT GGGAAATTTG 
GTCTTTACTG GCATCTTTGC AGCTGAAATG GTTTTAAAAC TAATTGCCAT GGATCCATAT 
GAGTATTTCC AAATAGGATG GAATATTTTT GATAGTCTTA TTGTGACCTT AAGCTTAGTG 
GAACTTTTTC TATCAGATGT GGAAGGATTG TCAGTTCTAC GATCATTCAG ACTGCTCCGA 
GTTTTCAAGT TAGCAAAATC TTGGCCAACA CTGAACATGC TGATCAAGAT CATTGGCAAT 
TCAGTGGGGG CTCTGGGAAA CCTAACCTTG GTGTTGGCCA TCATCGTCTT CATTTTTGCT 
GTGGTTGGCA TGCAGCTCTT TGGTAAGAGC TACAAAGAAT GTGTTTGCAA GATCAATGAA 
GACTGCACAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC 
CGGGTGCTGT GTGGAGAGTG GATAGAGACC ATGTGGGACT GCATGGAGGT CGCCGGTCAA 
GCCATGTGCC TTATTGTTTA CATGATGGTC ATGGTCATTG GAAACCTGGT GGTCCTCAAC 
CTATTTCTTG CTTTATTACT GAGCTCATTT AGTTCAGACA ATCTTACAGC CATTGAAGAA 
GACACTGATG CAAACAACCT CCAGATTGCA GTGGCCAGAA TTAAGAAGGG AATAAATTAC 
GTGAAACAAA CCTTACGGGA GTTTGTTCTA AAAGCATTTT CCAAAAAGCC AAAGATTTCC 
AAAGAGATTA GACAAACAGA AGATCTAAAT TGTAAAAAGG AAAACTACAT CTCTAACCGC 
ACACTTGCTG AAATGAGCAA GGATCACAAA TTCCACAAAG AAAAAGATAA AACCAGTGGT 
TTTGGAAACA GCATGGACAA ATACTTAATG GAAGAAAGTG ATGGTCAGTC ATTTATTCAT 
AATCCCAGCC TCACAGTGAC AGTACCAATT GCACCTGGGG AATCTGATTT GGAAATTATG 
AACACTGAGG AACTTAGCAG TGATTCAGAT AGCGAATACA GCAAAGGGAG GCTGAACCAA 
TCAAGTTCCT CAGAGTGCAG CACAGTTGAT AACCCTGTGC CTGGAGAAGG AGAAGAGGCA 
GAGGCTGAGC CTGTAAATTC AGATGAGCCA GAGGCCTGTT TTACCGATGG TTGCGTACAG 
AGGTTCCCAT GCTGCCAAGT TAACATAGAA TCAGGGAAAG GAAAAATCTG GTGGAACATC 
AGGAAAACGT GCTTCAGGAT AGTTGAGCAC AGTTGGTTTG AAAGCTTCAT TGTTCTCATG 
ATCTTGCTCA GCAGTGGTGC TCTGGCTTTT GAAGATATAT ATATTGAAAA GAAAAAGAAC 
ATTAAGATTA TCCTGGAGTA TGCTGACAAG ATATTCACGT ATATCTTCAT TCTAGAAATG 
CTTCTAAAAT GGGTAGCATA TGGTTACAAA ACCTATTTCA CCAATGCCTG GTGTTGGCTG 
GATTTCTTAA TTGTCGATGT TTCTTTGGTT ACATTAGTTG CAAACACTCT TGGCTACTCA 
GATCTTGGTC CCATTAAATC CCTTCGGACA CTTAGAGCTT TAAGACCTCT GCGAGCTTTA 
TCTAGATTTG AAGGAATGAG GGTGGTTGTG AATGCACTCA TCGGTGCAAT TCCTTCTATC 
ATGAATGTGC TACTTGTGTG TCTCATATTC TGGCTGATAT TTAGCATCAT GGGAGTAAAT 
TTGTTTGCTG GCAAGTTTTA TGAGTGTATC AACACCACAA ATGGATTACG TTTTCCTACA 
AGTGAAGTGG AAAACCGTTC TGCCTGTCTT GCGCTTATGA ATGTCAGTCA AAATGTGCGA 
TGGAAAAACC TAAAAGTGAA CTTTGATAAT GTTGGACTTG GTTACCTGTC TCTACTTCAA 
GTGGCAACAT TTAAGGGATG GATGGATATT ATGTATGCAG CAGTTGATTC TGTTAACGTA 
GACAAGCAGC CCATTTATGA ATACAGCCTC TACATGTATA TTTATTTTGT CATCTTCATC 
ATCTTTGGGT CATTCTTCAC TTTGAACTTG TTCATTGGTG TCATCATAGA TAATTTCAAC 
CAACAGAAAA AGAAGCTTGG AGGTCAAGAT ATCTTTATGA CAGAAGAACA GAAGAAATAT 
TATAATGCAA TGAAAAAGCT GGGATCCAAG AAACCACAAA AGCCAATACC TCGGCCAGGG 
AACAAATTCC AAGGATGTAT CTTTGACCTA GTAACAAACC AAGCTTTTGA TATAGCCATC 
ATGGTTCTGA TCTGCCTCAA CATGGTAACC ATGATGGTGG AAAAAGAGGG ACAAAGTGAC 
TACGTGACTG AAGTTTTAAA TTGGATCAAT GTGGTTTTTA TTATCCTGTT CTCTGGAGAA 
TGCGTGCTGA AACTGATCTC CCTCAGATGC TACTACTTCA CTGTAGGCTG GAACATCTTT 
GATTTTGTGG TTGTAATTCT CTCCATTGTA GGTATGTTTC TGGCAGATTT GATAGAAAGG 
TATTTTGTGT CCCCTACCCT ATTCCGAGTG ATCCGTCTTG CCAGGATTGG CCGAATCCTG 
CGTCTGATCA AAGGGGCCAA GGGGATCCGC ACGCTGCTCT TTGCTTTGAT GATGTCCCTT 
CCTGCGCTGT TTAACATCGG CCTCCTGCTC TTCCTGGTCA TGTTCATCTA TGCCATCTTT 
GGAATGTCCA ATTTTGCCTA TGTTAAAAAG GAAGCTGGAA TTAATGACAT GTTCAACTTT 
GAGACCTTCG CCAACAGCAT GATCTGCCTG TTCCAGATTA CCACCTCTGC TGGCTGGGAT 
GGATTGCTGG CACCTATCCT CAATAGTAAA CCACCGGACT GTGACCCCAA AAAAGTTCAT 
CCTGGAAGCT CAGTTGAAGG AGACTGTGGG AACCCATCTG TCGGGATATT CTATTTTGTC 
AGTTACATCA TCATATCATT TCTGGTCGTG GTGAACATGT ACATTGCTGT CATCCTGGAG 
AACTTCAGTG TTGCTACTGA AGAAAGTACT GAGCCCCTGA GTGAGGACGA TTTTGAGATG 
TTCTATGAGG TTTGGGAGAA GTTTGATCCA GACGCGACCC AATTTATAGA ATATAGCAAA 
CTCTCTGATT TTGCAGCTGC CCTGGATCCT CCTCTTCTCA TAGCAAAACC GAACAAAGTC 
CAGCTCATTG CCATGGACCT GCCCATGGTC AGTGGGGACA GGATCCACTG CCTCGACATC 
TTATTTGCTT TTACAAAGCG TGTTTTGGGG GAAGGTGGAG AGATGGACTC TCTTCGTTCA 
CAGATGGAAG AAAGGTTCAT GTCTGCAAAT CCCTCTAAGG TGTCTTATGA GCCCATCACA 
ACTACACTAA AAAGAAAGCA AGAGGATGTG TCTGCTACAG TCATTCAGCG TGCTTACAGA 
CGTTACCGCC TACGGCAAAA TGTCAAAAAT ATATCAAGTA TATACATCAA AGATGGTGAC 
AGAGATGATG ATTTGCCCAA TAAAGAAGAT ATGGTTTTTG ATAATGTTAA TGAGAACTCA 
AGTCCAGAAA AAACAGGTGC AACTCCATCC ACTGTCTCTC CACCTTCTTA TGATAGTGTA 
ACAAAACCAG ACAGAGAGAA ATATGAAAAA GACAAAACAG AAAAGGAAGA CAAAGGGAAA 
GATGGCAAGG AAGGCAAAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Up-regulation of NaV1.7 sodium channels expression by tumor necrosis factor-? in cultured bovine adrenal chromaffin cells and rat dorsal root ganglion neurons.
Anesthesia and analgesia118(2)318-24(2014 Feb)
Tamura R,Nemoto T,Maruta T,Onizuka S,Yanagita T,Wada A,Murakami M,Tsuneyoshi I

Endothelin-1-induced down-regulation of NaV1.7 expression in adrenal chromaffin cells: attenuation of catecholamine secretion and tau dephosphorylation.
FEBS letters587(7)898-905(2013 Apr)
Nemoto T,Yanagita T,Maruta T,Sugita C,Satoh S,Kanai T,Wada A,Murakami M

Nav1.7-Ca2+ influx-induced increased phosphorylations of extracellular signal-regulated kinase (ERK) and p38 attenuate tau phosphorylation via glycogen synthase kinase-3beta: priming of Nav1.7 gating by ERK and p38.
European journal of pharmacology640(1-3)20-8(2010 Aug)
Nemoto T,Miyazaki S,Kanai T,Maruta T,Satoh S,Yoshikawa N,Yanagita T,Wada A

Nav1.7 sodium channel-induced Ca2+ influx decreases tau phosphorylation via glycogen synthase kinase-3beta in adrenal chromaffin cells.
Neurochemistry international54(8)497-505(2009 Jul)
Kanai T,Nemoto T,Yanagita T,Maruta T,Satoh S,Yoshikawa N,Wada A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SCN9A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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