EIF5B cDNA ORF clone, Bos taurus(cattle)

The following EIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220729 XM_003586607.5
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220730 XM_024999309.1
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220731 XM_024999310.1
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220732 XM_024999311.1
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa23755 XM_003586607.4
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa23755 XM_003582755.4
Latest version!
Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220729
    Clone ID Related Accession (Same CDS sequence) XM_003586607.5
    Accession Version XM_003586607.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586607.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586655.2
    CDS279..3938
    Translation

    Target ORF information:

    RefSeq Version XM_003586607.5
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586607.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220730
    Clone ID Related Accession (Same CDS sequence) XM_024999309.1
    Accession Version XM_024999309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAAACACG
    GTGCATATAG AAGTAAAAGA AAACCCTGAA GAGGAGGAGG AGGAGGAAGA GGAGGAGGAG
    GAGGAAGAAG AGAGTGAAGA CGAAGAGGAG GAAGAAGGAG ACAGTGAAGG CAGTGAAGGT
    GAAGATGATG ATGAAAAGGT GTCAGATGAG AAGGACGCTG GGAAAACATT GGATAAAAAG
    CCAAGCAAAG ACGTGAGCTC AGAATCTGAG GATGACTCTG ACGACGATCG AACTAAAGAA
    GAACGGGCTT ATGACAAAGC AAAACGGAGG ATTGAGAAAC GGCGACTTGA ACACAGTAAA
    AATGTAAATA CAGAGAAGCT GAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGATACA
    GGGAAGACAA AAATTCTAGA TAAGCTCCGT CACACACATG TACAAGATGG TGAAGCAGGT
    GGTATCACAC AGCAAATTGG TGCCACCAAT GTCCCTCTTG AAGCTATTAA TGAACAAACT
    AAGATGATTA AAAATTTTGA TCGAGAGAAC GTACGGATTC CAGGAATGCT GATTATTGAC
    ACTCCGGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGACATT
    GCCATTTTAG TTGTTGATAT TATGCATGGT TTGGAGCCCC AGACAATTGA ATCTATCAAC
    CTTTTGAAAT CTAAAAAATG TCCCTTCATT GTTGCCCTCA ATAAGATTGA TAGGTTGTAT
    GATTGGAAAA AGAGCCCTGA CTCTGACGTG GCCGCTACTC TGAAGAAGCA GAAGAAGAAC
    ACCAGAGATG AATTTGAGGA GCGGGCCAAG GCGATCATTG TGGAATTTGC ACAGCAGGGG
    CTGAATGCAG CTTTGTTCTA TGAGAATAAG GATCCCCGCA CTTTTGTGTC CTTGGTACCT
    ACCTCTGCAC ACACTGGCGA TGGCATGGGA AGTCTGATCT GCCTTCTTGT TGAGTTGACT
    CAGACCATGT TGAGTAAGAG GCTTGCCCAG TGTGAGGAGC TGAGAGCCCA GGTGATGGAG
    GTTAAGGCTC TCCCGGGAAT GGGCACGACT ATTGATGTAA TACTGATCAA CGGGCGTCTG
    AAGGAAGGAG ACACGATTAT TGTTCCCGGA GTAGAAGGGC CTATCGTCAC GCAGATCCGA
    GGCCTCCTGT TACCTCCCCC TATGAAGGAG TTAAGAGTGA AGAACCAATA TGAGAAGCAT
    AAAGAGGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AGGACCTGGA AAAAACATTG
    GCTGGTTTAC CCCTCCTCGT GGCTTATAAA GAAGATGAAG TCCCAGTTCT TAAAGATGAG
    CTGATCCACG AGTTAAAGCA GACACTGAAC GCTATCAAAT TAGAAGAGAA AGGCGTCTAC
    GTCCAGGCCT CGACCCTGGG ATCTCTGGAA GCTCTCCTTG AATTTCTGAA GACATCAGAA
    GTGCCCTACG CAGGGATCAA CATTGGGCCC GTCCACAAAA AAGACGTCAT GAAGGCTTCG
    GTGATGCTGG AACACGACCC TCAGTATGCA GTGATTTTGG CCTTCGATGT GAGAATTGAA
    CGGGATGCCC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT
    TATCATTTAT TTGATGCCTT TACGAAATAC AGACAAGACT ACAAGAAACA GAAACAAGAA
    GAATTTAAGC ACATAGCAGT ATTCCCCTGC AAGATAAAGA TCCTCCCTCA GTTTATCTTC
    AATTCTCGGG ATCCGATAGT GATGGGCGTG ACTGTGGAAG CAGGGCAGGT GAAGCAGGGG
    ACACCCATGT GCGTGCCCAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA
    GTAAACCATA AACAAGTGGA TGTTGCGAAA AAAGGCCAAG AAGTCTGTGT CAAAATAGAA
    CCCATCCCAG GTGAATCTCC CAAAATGTAT GGACGCCATT TTGAAGCTAC AGACATTCTC
    GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCTCTCAAAG ACTGGTTCAG AGACGAGATG
    CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855077.1
    CDS279..3935
    Translation

    Target ORF information:

    RefSeq Version XM_024999309.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA AACCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAAACACG
    GTGCATATAG AAGTAAAAGA AAACCCTGAA GAGGAGGAGG AGGAGGAAGA GGAGGAGGAG
    GAGGAAGAAG AGAGTGAAGA CGAAGAGGAG GAAGAAGGAG ACAGTGAAGG CAGTGAAGGT
    GAAGATGATG ATGAAAAGGT GTCAGATGAG AAGGACGCTG GGAAAACATT GGATAAAAAG
    CCAAGCAAAG ACGTGAGCTC AGAATCTGAG GATGACTCTG ACGACGATCG AACTAAAGAA
    GAACGGGCTT ATGACAAAGC AAAACGGAGG ATTGAGAAAC GGCGACTTGA ACACAGTAAA
    AATGTAAATA CAGAGAAGCT GAGAGCTCCT GTTATTTGTG TACTTGGACA TGTGGATACA
    GGGAAGACAA AAATTCTAGA TAAGCTCCGT CACACACATG TACAAGATGG TGAAGCAGGT
    GGTATCACAC AGCAAATTGG TGCCACCAAT GTCCCTCTTG AAGCTATTAA TGAACAAACT
    AAGATGATTA AAAATTTTGA TCGAGAGAAC GTACGGATTC CAGGAATGCT GATTATTGAC
    ACTCCGGGGC ATGAGTCTTT CAGTAATCTG AGAAACAGAG GAAGCTCTCT TTGTGACATT
    GCCATTTTAG TTGTTGATAT TATGCATGGT TTGGAGCCCC AGACAATTGA ATCTATCAAC
    CTTTTGAAAT CTAAAAAATG TCCCTTCATT GTTGCCCTCA ATAAGATTGA TAGGTTGTAT
    GATTGGAAAA AGAGCCCTGA CTCTGACGTG GCCGCTACTC TGAAGAAGCA GAAGAAGAAC
    ACCAGAGATG AATTTGAGGA GCGGGCCAAG GCGATCATTG TGGAATTTGC ACAGCAGGGG
    CTGAATGCAG CTTTGTTCTA TGAGAATAAG GATCCCCGCA CTTTTGTGTC CTTGGTACCT
    ACCTCTGCAC ACACTGGCGA TGGCATGGGA AGTCTGATCT GCCTTCTTGT TGAGTTGACT
    CAGACCATGT TGAGTAAGAG GCTTGCCCAG TGTGAGGAGC TGAGAGCCCA GGTGATGGAG
    GTTAAGGCTC TCCCGGGAAT GGGCACGACT ATTGATGTAA TACTGATCAA CGGGCGTCTG
    AAGGAAGGAG ACACGATTAT TGTTCCCGGA GTAGAAGGGC CTATCGTCAC GCAGATCCGA
    GGCCTCCTGT TACCTCCCCC TATGAAGGAG TTAAGAGTGA AGAACCAATA TGAGAAGCAT
    AAAGAGGTAG AAGCAGCTCA GGGGGTAAAG ATTCTTGGAA AGGACCTGGA AAAAACATTG
    GCTGGTTTAC CCCTCCTCGT GGCTTATAAA GAAGATGAAG TCCCAGTTCT TAAAGATGAG
    CTGATCCACG AGTTAAAGCA GACACTGAAC GCTATCAAAT TAGAAGAGAA AGGCGTCTAC
    GTCCAGGCCT CGACCCTGGG ATCTCTGGAA GCTCTCCTTG AATTTCTGAA GACATCAGAA
    GTGCCCTACG CAGGGATCAA CATTGGGCCC GTCCACAAAA AAGACGTCAT GAAGGCTTCG
    GTGATGCTGG AACACGACCC TCAGTATGCA GTGATTTTGG CCTTCGATGT GAGAATTGAA
    CGGGATGCCC AAGAAATGGC TGATAGTTTA GGAGTTAGAA TTTTTAGTGC AGAAATTATT
    TATCATTTAT TTGATGCCTT TACGAAATAC AGACAAGACT ACAAGAAACA GAAACAAGAA
    GAATTTAAGC ACATAGCAGT ATTCCCCTGC AAGATAAAGA TCCTCCCTCA GTTTATCTTC
    AATTCTCGGG ATCCGATAGT GATGGGCGTG ACTGTGGAAG CAGGGCAGGT GAAGCAGGGG
    ACACCCATGT GCGTGCCCAG CAAAAATTTT GTTGACATTG GAATAGTAAC AAGTATTGAA
    GTAAACCATA AACAAGTGGA TGTTGCGAAA AAAGGCCAAG AAGTCTGTGT CAAAATAGAA
    CCCATCCCAG GTGAATCTCC CAAAATGTAT GGACGCCATT TTGAAGCTAC AGACATTCTC
    GTCAGTAAGA TCAGCCGGCA GTCCATCGAT GCTCTCAAAG ACTGGTTCAG AGACGAGATG
    CAGAAGAGTG ACTGGCAGCT TATTGTGGAG CTGAAGAAAG TGTTTGAAAT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220731
    Clone ID Related Accession (Same CDS sequence) XM_024999310.1
    Accession Version XM_024999310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAAATT GCCTTTTATT TCTTGCCAAG GATGACATTG ATCTTGATGC CTTGGCTGCA 
    GAAATAGAAG GAGCTGGTGC TGCCAGGGAG CAAGAACCTC AAAAGGCAAA GGGGAAAAAG
    AAAAAGGAGA AAAAAAGACA AGACTTTGAT GAAGATGATA TCCTGAAAGA ACTGGAAGAA
    TTGTCTTGGG AAGCTCAAGG CATCAAAGCT GACAGAGAGC CTGCTGCGGT CAAGCCAACA
    GAAAATAATG AAGATGAACC CATCTCAAAA CAAGATAAGA AAAAGAAAGG ACAGAAAGGC
    AAAAAACAGA GTTTTGAAGA TAATGATAGT GAAGAATTGG AAGAAAAAGA TTCAAAGTCA
    AAAAAGACTG CAAAACCCAA AGTGGAAATG TACTCTGGGA GTGATGATGA TGATGATTTT
    AATAAACTAT CCAAAAAAGC AAAGGGGAAA GCTCAGAAAT CAAATAAGAA GCGTGATGCA
    TCAGAAGAGG ATGAGGATAA CAGTAAAAGA ATTAAAGAGC GTATGAGAGC ACATTCTTCT
    GGTGAAAGTG GTGATGAATC AGATGAATTT TTGCAATCCA GGAAAGGACA GAAAAAAAAT
    CAGAAAAACA AGCCAGGTCC TACTGTCGAG AGTGGAAATG AAGATGATGA CTCGTCCTTC
    AAAATTAAGA CAGTGGCTCA AAAGAAAGCA GAGAAGAAAG AGCGTGAGAG AAAAAAACGG
    GATGAAGAAA AGGCAAAACT TCGGAAGCTG AAAGAAAAAG AAGAGCTGGA GTCTGGTAAA
    AAGGATCTGG GTAAACCAAA AGAATCTCAG CGGAAACCTG AGGAGGAAGC TCTGAAATCC
    AAAGTGACAC TTGACTCTGG GGCAGCTCCT GCCTCTGAAG AGAAAGGAGA GGGTCCCATA
    GGCGCAGAAG ATGACAATGA AGGAGACAAA AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA
    GAAAAGGAAG AAAAGGAGAA AGAAAAGAAG AAAGGACCTA GCAAAGCCAC AGTTAAGGCT
    ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA GAGGAGGAAA GACAGAAGAG AGAGGAGGAA
    GAACGGATAA AACGGCTTGA AGAGTTAGAA GCCAAGCGTA AAGAAGAGGA AAGATTGGAA
    CAAGAAAAAA GAGAAAGGAA AAAGCAAAAA GAAAAGGAAA GGAAAGAACG CTTGAAAAAA
    GAGGGGAAAC TTTTAACTAA ATCCCAGAGA GAAGCCAGAG CCAGAGCCGA AGCTACCCTT
    AAACTTCTAC AAGCTCAGGG TGTTGAAGTG CCATCAAAAG ACTCTTTGCC AAAGAAGAGG
    CCAATTTATG AAGATAAAAA GAAGAAAAAA CCACCGCAGC AGATGGAAAA TAAAGTTTCT
    GAACCAGTGG AATTAAGTGC TGCTGCAGAA GTTGTGGAGC AAGGACTACC CGAAAAAGAA
    GAGACACCGC CTCCTGTTGA ACCAGAAGAG GAAGAAGAAA CCGAGGATGC TGGGTTGGAT
    GACTGGGAAG CTATGGCTAG CGATGAGGAG AGAGAGACAG AAGAAAACAC GGTGCATATA
    GAAGTAAAAG AAAACCCTGA AGAGGAGGAG GAGGAGGAAG AGGAGGAGGA GGAGGAAGAA
    GAGAGTGAAG ACGAAGAGGA GGAAGAAGGA GACAGTGAAG GCAGTGAAGG TGAAGATGAT
    GATGAAAAGG TGTCAGATGA GAAGGACGCT GGGAAAACAT TGGATAAAAA GCCAAGCAAA
    GACGTGAGCT CAGAATCTGA GGATGACTCT GACGACGATC GAACTAAAGA AGAACGGGCT
    TATGACAAAG CAAAACGGAG GATTGAGAAA CGGCGACTTG AACACAGTAA AAATGTAAAT
    ACAGAGAAGC TGAGAGCTCC TGTTATTTGT GTACTTGGAC ATGTGGATAC AGGGAAGACA
    AAAATTCTAG ATAAGCTCCG TCACACACAT GTACAAGATG GTGAAGCAGG TGGTATCACA
    CAGCAAATTG GTGCCACCAA TGTCCCTCTT GAAGCTATTA ATGAACAAAC TAAGATGATT
    AAAAATTTTG ATCGAGAGAA CGTACGGATT CCAGGAATGC TGATTATTGA CACTCCGGGG
    CATGAGTCTT TCAGTAATCT GAGAAACAGA GGAAGCTCTC TTTGTGACAT TGCCATTTTA
    GTTGTTGATA TTATGCATGG TTTGGAGCCC CAGACAATTG AATCTATCAA CCTTTTGAAA
    TCTAAAAAAT GTCCCTTCAT TGTTGCCCTC AATAAGATTG ATAGGTTGTA TGATTGGAAA
    AAGAGCCCTG ACTCTGACGT GGCCGCTACT CTGAAGAAGC AGAAGAAGAA CACCAGAGAT
    GAATTTGAGG AGCGGGCCAA GGCGATCATT GTGGAATTTG CACAGCAGGG GCTGAATGCA
    GCTTTGTTCT ATGAGAATAA GGATCCCCGC ACTTTTGTGT CCTTGGTACC TACCTCTGCA
    CACACTGGCG ATGGCATGGG AAGTCTGATC TGCCTTCTTG TTGAGTTGAC TCAGACCATG
    TTGAGTAAGA GGCTTGCCCA GTGTGAGGAG CTGAGAGCCC AGGTGATGGA GGTTAAGGCT
    CTCCCGGGAA TGGGCACGAC TATTGATGTA ATACTGATCA ACGGGCGTCT GAAGGAAGGA
    GACACGATTA TTGTTCCCGG AGTAGAAGGG CCTATCGTCA CGCAGATCCG AGGCCTCCTG
    TTACCTCCCC CTATGAAGGA GTTAAGAGTG AAGAACCAAT ATGAGAAGCA TAAAGAGGTA
    GAAGCAGCTC AGGGGGTAAA GATTCTTGGA AAGGACCTGG AAAAAACATT GGCTGGTTTA
    CCCCTCCTCG TGGCTTATAA AGAAGATGAA GTCCCAGTTC TTAAAGATGA GCTGATCCAC
    GAGTTAAAGC AGACACTGAA CGCTATCAAA TTAGAAGAGA AAGGCGTCTA CGTCCAGGCC
    TCGACCCTGG GATCTCTGGA AGCTCTCCTT GAATTTCTGA AGACATCAGA AGTGCCCTAC
    GCAGGGATCA ACATTGGGCC CGTCCACAAA AAAGACGTCA TGAAGGCTTC GGTGATGCTG
    GAACACGACC CTCAGTATGC AGTGATTTTG GCCTTCGATG TGAGAATTGA ACGGGATGCC
    CAAGAAATGG CTGATAGTTT AGGAGTTAGA ATTTTTAGTG CAGAAATTAT TTATCATTTA
    TTTGATGCCT TTACGAAATA CAGACAAGAC TACAAGAAAC AGAAACAAGA AGAATTTAAG
    CACATAGCAG TATTCCCCTG CAAGATAAAG ATCCTCCCTC AGTTTATCTT CAATTCTCGG
    GATCCGATAG TGATGGGCGT GACTGTGGAA GCAGGGCAGG TGAAGCAGGG GACACCCATG
    TGCGTGCCCA GCAAAAATTT TGTTGACATT GGAATAGTAA CAAGTATTGA AGTAAACCAT
    AAACAAGTGG ATGTTGCGAA AAAAGGCCAA GAAGTCTGTG TCAAAATAGA ACCCATCCCA
    GGTGAATCTC CCAAAATGTA TGGACGCCAT TTTGAAGCTA CAGACATTCT CGTCAGTAAG
    ATCAGCCGGC AGTCCATCGA TGCTCTCAAA GACTGGTTCA GAGACGAGAT GCAGAAGAGT
    GACTGGCAGC TTATTGTGGA GCTGAAGAAA GTGTTTGAAA TCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855078.1
    CDS19..3666
    Translation

    Target ORF information:

    RefSeq Version XM_024999310.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAAAATT GCCTTTTATT TCTTGCCAAG GATGACATTG ATCTTGATGC CTTGGCTGCA 
    GAAATAGAAG GAGCTGGTGC TGCCAGGGAG CAAGAACCTC AAAAGGCAAA GGGGAAAAAG
    AAAAAGGAGA AAAAAAGACA AGACTTTGAT GAAGATGATA TCCTGAAAGA ACTGGAAGAA
    TTGTCTTGGG AAGCTCAAGG CATCAAAGCT GACAGAGAGC CTGCTGCGGT CAAGCCAACA
    GAAAATAATG AAGATGAACC CATCTCAAAA CAAGATAAGA AAAAGAAAGG ACAGAAAGGC
    AAAAAACAGA GTTTTGAAGA TAATGATAGT GAAGAATTGG AAGAAAAAGA TTCAAAGTCA
    AAAAAGACTG CAAAACCCAA AGTGGAAATG TACTCTGGGA GTGATGATGA TGATGATTTT
    AATAAACTAT CCAAAAAAGC AAAGGGGAAA GCTCAGAAAT CAAATAAGAA GCGTGATGCA
    TCAGAAGAGG ATGAGGATAA CAGTAAAAGA ATTAAAGAGC GTATGAGAGC ACATTCTTCT
    GGTGAAAGTG GTGATGAATC AGATGAATTT TTGCAATCCA GGAAAGGACA GAAAAAAAAT
    CAGAAAAACA AGCCAGGTCC TACTGTCGAG AGTGGAAATG AAGATGATGA CTCGTCCTTC
    AAAATTAAGA CAGTGGCTCA AAAGAAAGCA GAGAAGAAAG AGCGTGAGAG AAAAAAACGG
    GATGAAGAAA AGGCAAAACT TCGGAAGCTG AAAGAAAAAG AAGAGCTGGA GTCTGGTAAA
    AAGGATCTGG GTAAACCAAA AGAATCTCAG CGGAAACCTG AGGAGGAAGC TCTGAAATCC
    AAAGTGACAC TTGACTCTGG GGCAGCTCCT GCCTCTGAAG AGAAAGGAGA GGGTCCCATA
    GGCGCAGAAG ATGACAATGA AGGAGACAAA AAGAAAAAAG ATAAGAAGAA AAAGAAAGGA
    GAAAAGGAAG AAAAGGAGAA AGAAAAGAAG AAAGGACCTA GCAAAGCCAC AGTTAAGGCT
    ATGCAAGAAG CTCTGGCTAA GCTTAAAGAA GAGGAGGAAA GACAGAAGAG AGAGGAGGAA
    GAACGGATAA AACGGCTTGA AGAGTTAGAA GCCAAGCGTA AAGAAGAGGA AAGATTGGAA
    CAAGAAAAAA GAGAAAGGAA AAAGCAAAAA GAAAAGGAAA GGAAAGAACG CTTGAAAAAA
    GAGGGGAAAC TTTTAACTAA ATCCCAGAGA GAAGCCAGAG CCAGAGCCGA AGCTACCCTT
    AAACTTCTAC AAGCTCAGGG TGTTGAAGTG CCATCAAAAG ACTCTTTGCC AAAGAAGAGG
    CCAATTTATG AAGATAAAAA GAAGAAAAAA CCACCGCAGC AGATGGAAAA TAAAGTTTCT
    GAACCAGTGG AATTAAGTGC TGCTGCAGAA GTTGTGGAGC AAGGACTACC CGAAAAAGAA
    GAGACACCGC CTCCTGTTGA ACCAGAAGAG GAAGAAGAAA CCGAGGATGC TGGGTTGGAT
    GACTGGGAAG CTATGGCTAG CGATGAGGAG AGAGAGACAG AAGAAAACAC GGTGCATATA
    GAAGTAAAAG AAAACCCTGA AGAGGAGGAG GAGGAGGAAG AGGAGGAGGA GGAGGAAGAA
    GAGAGTGAAG ACGAAGAGGA GGAAGAAGGA GACAGTGAAG GCAGTGAAGG TGAAGATGAT
    GATGAAAAGG TGTCAGATGA GAAGGACGCT GGGAAAACAT TGGATAAAAA GCCAAGCAAA
    GACGTGAGCT CAGAATCTGA GGATGACTCT GACGACGATC GAACTAAAGA AGAACGGGCT
    TATGACAAAG CAAAACGGAG GATTGAGAAA CGGCGACTTG AACACAGTAA AAATGTAAAT
    ACAGAGAAGC TGAGAGCTCC TGTTATTTGT GTACTTGGAC ATGTGGATAC AGGGAAGACA
    AAAATTCTAG ATAAGCTCCG TCACACACAT GTACAAGATG GTGAAGCAGG TGGTATCACA
    CAGCAAATTG GTGCCACCAA TGTCCCTCTT GAAGCTATTA ATGAACAAAC TAAGATGATT
    AAAAATTTTG ATCGAGAGAA CGTACGGATT CCAGGAATGC TGATTATTGA CACTCCGGGG
    CATGAGTCTT TCAGTAATCT GAGAAACAGA GGAAGCTCTC TTTGTGACAT TGCCATTTTA
    GTTGTTGATA TTATGCATGG TTTGGAGCCC CAGACAATTG AATCTATCAA CCTTTTGAAA
    TCTAAAAAAT GTCCCTTCAT TGTTGCCCTC AATAAGATTG ATAGGTTGTA TGATTGGAAA
    AAGAGCCCTG ACTCTGACGT GGCCGCTACT CTGAAGAAGC AGAAGAAGAA CACCAGAGAT
    GAATTTGAGG AGCGGGCCAA GGCGATCATT GTGGAATTTG CACAGCAGGG GCTGAATGCA
    GCTTTGTTCT ATGAGAATAA GGATCCCCGC ACTTTTGTGT CCTTGGTACC TACCTCTGCA
    CACACTGGCG ATGGCATGGG AAGTCTGATC TGCCTTCTTG TTGAGTTGAC TCAGACCATG
    TTGAGTAAGA GGCTTGCCCA GTGTGAGGAG CTGAGAGCCC AGGTGATGGA GGTTAAGGCT
    CTCCCGGGAA TGGGCACGAC TATTGATGTA ATACTGATCA ACGGGCGTCT GAAGGAAGGA
    GACACGATTA TTGTTCCCGG AGTAGAAGGG CCTATCGTCA CGCAGATCCG AGGCCTCCTG
    TTACCTCCCC CTATGAAGGA GTTAAGAGTG AAGAACCAAT ATGAGAAGCA TAAAGAGGTA
    GAAGCAGCTC AGGGGGTAAA GATTCTTGGA AAGGACCTGG AAAAAACATT GGCTGGTTTA
    CCCCTCCTCG TGGCTTATAA AGAAGATGAA GTCCCAGTTC TTAAAGATGA GCTGATCCAC
    GAGTTAAAGC AGACACTGAA CGCTATCAAA TTAGAAGAGA AAGGCGTCTA CGTCCAGGCC
    TCGACCCTGG GATCTCTGGA AGCTCTCCTT GAATTTCTGA AGACATCAGA AGTGCCCTAC
    GCAGGGATCA ACATTGGGCC CGTCCACAAA AAAGACGTCA TGAAGGCTTC GGTGATGCTG
    GAACACGACC CTCAGTATGC AGTGATTTTG GCCTTCGATG TGAGAATTGA ACGGGATGCC
    CAAGAAATGG CTGATAGTTT AGGAGTTAGA ATTTTTAGTG CAGAAATTAT TTATCATTTA
    TTTGATGCCT TTACGAAATA CAGACAAGAC TACAAGAAAC AGAAACAAGA AGAATTTAAG
    CACATAGCAG TATTCCCCTG CAAGATAAAG ATCCTCCCTC AGTTTATCTT CAATTCTCGG
    GATCCGATAG TGATGGGCGT GACTGTGGAA GCAGGGCAGG TGAAGCAGGG GACACCCATG
    TGCGTGCCCA GCAAAAATTT TGTTGACATT GGAATAGTAA CAAGTATTGA AGTAAACCAT
    AAACAAGTGG ATGTTGCGAA AAAAGGCCAA GAAGTCTGTG TCAAAATAGA ACCCATCCCA
    GGTGAATCTC CCAAAATGTA TGGACGCCAT TTTGAAGCTA CAGACATTCT CGTCAGTAAG
    ATCAGCCGGC AGTCCATCGA TGCTCTCAAA GACTGGTTCA GAGACGAGAT GCAGAAGAGT
    GACTGGCAGC TTATTGTGGA GCTGAAGAAA GTGTTTGAAA TCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220732
    Clone ID Related Accession (Same CDS sequence) XM_024999311.1
    Accession Version XM_024999311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 5B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTACTCTG GGAGTGATGA TGATGATGAT TTTAATAAAC TATCCAAAAA AGCAAAGGGG 
    AAAGCTCAGA AATCAAATAA GAAGCGTGAT GCATCAGAAG AGGATGAGGA TAACAGTAAA
    AGAATTAAAG AGCGTATGAG AGCACATTCT TCTGGTGAAA GTGGTGATGA ATCAGATGAA
    TTTTTGCAAT CCAGGAAAGG ACAGAAAAAA AATCAGAAAA ACAAGCCAGG TCCTACTGTC
    GAGAGTGGAA ATGAAGATGA TGACTCGTCC TTCAAAATTA AGACAGTGGC TCAAAAGAAA
    GCAGAGAAGA AAGAGCGTGA GAGAAAAAAA CGGGATGAAG AAAAGGCAAA ACTTCGGAAG
    CTGAAAGAAA AAGAAGAGCT GGAGTCTGGT AAAAAGGATC TGGGTAAACC AAAAGAATCT
    CAGCGGAAAC CTGAGGAGGA AGCTCTGAAA TCCAAAGTGA CACTTGACTC TGGGGCAGCT
    CCTGCCTCTG AAGAGAAAGG AGAGGGTCCC ATAGGCGCAG AAGATGACAA TGAAGGAGAC
    AAAAAGAAAA AAGATAAGAA GAAAAAGAAA GGAGAAAAGG AAGAAAAGGA GAAAGAAAAG
    AAGAAAGGAC CTAGCAAAGC CACAGTTAAG GCTATGCAAG AAGCTCTGGC TAAGCTTAAA
    GAAGAGGAGG AAAGACAGAA GAGAGAGGAG GAAGAACGGA TAAAACGGCT TGAAGAGTTA
    GAAGCCAAGC GTAAAGAAGA GGAAAGATTG GAACAAGAAA AAAGAGAAAG GAAAAAGCAA
    AAAGAAAAGG AAAGGAAAGA ACGCTTGAAA AAAGAGGGGA AACTTTTAAC TAAATCCCAG
    AGAGAAGCCA GAGCCAGAGC CGAAGCTACC CTTAAACTTC TACAAGCTCA GGGTGTTGAA
    GTGCCATCAA AAGACTCTTT GCCAAAGAAG AGGCCAATTT ATGAAGATAA AAAGAAGAAA
    AAACCACCGC AGCAGATGGA AAATAAAGTT TCTGAACCAG TGGAATTAAG TGCTGCTGCA
    GAAGTTGTGG AGCAAGGACT ACCCGAAAAA GAAGAGACAC CGCCTCCTGT TGAACCAGAA
    GAGGAAGAAG AAACCGAGGA TGCTGGGTTG GATGACTGGG AAGCTATGGC TAGCGATGAG
    GAGAGAGAGA CAGAAGAAAA CACGGTGCAT ATAGAAGTAA AAGAAAACCC TGAAGAGGAG
    GAGGAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAGAGTG AAGACGAAGA GGAGGAAGAA
    GGAGACAGTG AAGGCAGTGA AGGTGAAGAT GATGATGAAA AGGTGTCAGA TGAGAAGGAC
    GCTGGGAAAA CATTGGATAA AAAGCCAAGC AAAGACGTGA GCTCAGAATC TGAGGATGAC
    TCTGACGACG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCAAAACG GAGGATTGAG
    AAACGGCGAC TTGAACACAG TAAAAATGTA AATACAGAGA AGCTGAGAGC TCCTGTTATT
    TGTGTACTTG GACATGTGGA TACAGGGAAG ACAAAAATTC TAGATAAGCT CCGTCACACA
    CATGTACAAG ATGGTGAAGC AGGTGGTATC ACACAGCAAA TTGGTGCCAC CAATGTCCCT
    CTTGAAGCTA TTAATGAACA AACTAAGATG ATTAAAAATT TTGATCGAGA GAACGTACGG
    ATTCCAGGAA TGCTGATTAT TGACACTCCG GGGCATGAGT CTTTCAGTAA TCTGAGAAAC
    AGAGGAAGCT CTCTTTGTGA CATTGCCATT TTAGTTGTTG ATATTATGCA TGGTTTGGAG
    CCCCAGACAA TTGAATCTAT CAACCTTTTG AAATCTAAAA AATGTCCCTT CATTGTTGCC
    CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA CGTGGCCGCT
    ACTCTGAAGA AGCAGAAGAA GAACACCAGA GATGAATTTG AGGAGCGGGC CAAGGCGATC
    ATTGTGGAAT TTGCACAGCA GGGGCTGAAT GCAGCTTTGT TCTATGAGAA TAAGGATCCC
    CGCACTTTTG TGTCCTTGGT ACCTACCTCT GCACACACTG GCGATGGCAT GGGAAGTCTG
    ATCTGCCTTC TTGTTGAGTT GACTCAGACC ATGTTGAGTA AGAGGCTTGC CCAGTGTGAG
    GAGCTGAGAG CCCAGGTGAT GGAGGTTAAG GCTCTCCCGG GAATGGGCAC GACTATTGAT
    GTAATACTGA TCAACGGGCG TCTGAAGGAA GGAGACACGA TTATTGTTCC CGGAGTAGAA
    GGGCCTATCG TCACGCAGAT CCGAGGCCTC CTGTTACCTC CCCCTATGAA GGAGTTAAGA
    GTGAAGAACC AATATGAGAA GCATAAAGAG GTAGAAGCAG CTCAGGGGGT AAAGATTCTT
    GGAAAGGACC TGGAAAAAAC ATTGGCTGGT TTACCCCTCC TCGTGGCTTA TAAAGAAGAT
    GAAGTCCCAG TTCTTAAAGA TGAGCTGATC CACGAGTTAA AGCAGACACT GAACGCTATC
    AAATTAGAAG AGAAAGGCGT CTACGTCCAG GCCTCGACCC TGGGATCTCT GGAAGCTCTC
    CTTGAATTTC TGAAGACATC AGAAGTGCCC TACGCAGGGA TCAACATTGG GCCCGTCCAC
    AAAAAAGACG TCATGAAGGC TTCGGTGATG CTGGAACACG ACCCTCAGTA TGCAGTGATT
    TTGGCCTTCG ATGTGAGAAT TGAACGGGAT GCCCAAGAAA TGGCTGATAG TTTAGGAGTT
    AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATACAGACAA
    GACTACAAGA AACAGAAACA AGAAGAATTT AAGCACATAG CAGTATTCCC CTGCAAGATA
    AAGATCCTCC CTCAGTTTAT CTTCAATTCT CGGGATCCGA TAGTGATGGG CGTGACTGTG
    GAAGCAGGGC AGGTGAAGCA GGGGACACCC ATGTGCGTGC CCAGCAAAAA TTTTGTTGAC
    ATTGGAATAG TAACAAGTAT TGAAGTAAAC CATAAACAAG TGGATGTTGC GAAAAAAGGC
    CAAGAAGTCT GTGTCAAAAT AGAACCCATC CCAGGTGAAT CTCCCAAAAT GTATGGACGC
    CATTTTGAAG CTACAGACAT TCTCGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCTCTC
    AAAGACTGGT TCAGAGACGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG
    AAAGTGTTTG AAATCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855079.1
    CDS489..3749
    Translation

    Target ORF information:

    RefSeq Version XM_024999311.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTACTCTG GGAGTGATGA TGATGATGAT TTTAATAAAC TATCCAAAAA AGCAAAGGGG 
    AAAGCTCAGA AATCAAATAA GAAGCGTGAT GCATCAGAAG AGGATGAGGA TAACAGTAAA
    AGAATTAAAG AGCGTATGAG AGCACATTCT TCTGGTGAAA GTGGTGATGA ATCAGATGAA
    TTTTTGCAAT CCAGGAAAGG ACAGAAAAAA AATCAGAAAA ACAAGCCAGG TCCTACTGTC
    GAGAGTGGAA ATGAAGATGA TGACTCGTCC TTCAAAATTA AGACAGTGGC TCAAAAGAAA
    GCAGAGAAGA AAGAGCGTGA GAGAAAAAAA CGGGATGAAG AAAAGGCAAA ACTTCGGAAG
    CTGAAAGAAA AAGAAGAGCT GGAGTCTGGT AAAAAGGATC TGGGTAAACC AAAAGAATCT
    CAGCGGAAAC CTGAGGAGGA AGCTCTGAAA TCCAAAGTGA CACTTGACTC TGGGGCAGCT
    CCTGCCTCTG AAGAGAAAGG AGAGGGTCCC ATAGGCGCAG AAGATGACAA TGAAGGAGAC
    AAAAAGAAAA AAGATAAGAA GAAAAAGAAA GGAGAAAAGG AAGAAAAGGA GAAAGAAAAG
    AAGAAAGGAC CTAGCAAAGC CACAGTTAAG GCTATGCAAG AAGCTCTGGC TAAGCTTAAA
    GAAGAGGAGG AAAGACAGAA GAGAGAGGAG GAAGAACGGA TAAAACGGCT TGAAGAGTTA
    GAAGCCAAGC GTAAAGAAGA GGAAAGATTG GAACAAGAAA AAAGAGAAAG GAAAAAGCAA
    AAAGAAAAGG AAAGGAAAGA ACGCTTGAAA AAAGAGGGGA AACTTTTAAC TAAATCCCAG
    AGAGAAGCCA GAGCCAGAGC CGAAGCTACC CTTAAACTTC TACAAGCTCA GGGTGTTGAA
    GTGCCATCAA AAGACTCTTT GCCAAAGAAG AGGCCAATTT ATGAAGATAA AAAGAAGAAA
    AAACCACCGC AGCAGATGGA AAATAAAGTT TCTGAACCAG TGGAATTAAG TGCTGCTGCA
    GAAGTTGTGG AGCAAGGACT ACCCGAAAAA GAAGAGACAC CGCCTCCTGT TGAACCAGAA
    GAGGAAGAAG AAACCGAGGA TGCTGGGTTG GATGACTGGG AAGCTATGGC TAGCGATGAG
    GAGAGAGAGA CAGAAGAAAA CACGGTGCAT ATAGAAGTAA AAGAAAACCC TGAAGAGGAG
    GAGGAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAGAGTG AAGACGAAGA GGAGGAAGAA
    GGAGACAGTG AAGGCAGTGA AGGTGAAGAT GATGATGAAA AGGTGTCAGA TGAGAAGGAC
    GCTGGGAAAA CATTGGATAA AAAGCCAAGC AAAGACGTGA GCTCAGAATC TGAGGATGAC
    TCTGACGACG ATCGAACTAA AGAAGAACGG GCTTATGACA AAGCAAAACG GAGGATTGAG
    AAACGGCGAC TTGAACACAG TAAAAATGTA AATACAGAGA AGCTGAGAGC TCCTGTTATT
    TGTGTACTTG GACATGTGGA TACAGGGAAG ACAAAAATTC TAGATAAGCT CCGTCACACA
    CATGTACAAG ATGGTGAAGC AGGTGGTATC ACACAGCAAA TTGGTGCCAC CAATGTCCCT
    CTTGAAGCTA TTAATGAACA AACTAAGATG ATTAAAAATT TTGATCGAGA GAACGTACGG
    ATTCCAGGAA TGCTGATTAT TGACACTCCG GGGCATGAGT CTTTCAGTAA TCTGAGAAAC
    AGAGGAAGCT CTCTTTGTGA CATTGCCATT TTAGTTGTTG ATATTATGCA TGGTTTGGAG
    CCCCAGACAA TTGAATCTAT CAACCTTTTG AAATCTAAAA AATGTCCCTT CATTGTTGCC
    CTCAATAAGA TTGATAGGTT GTATGATTGG AAAAAGAGCC CTGACTCTGA CGTGGCCGCT
    ACTCTGAAGA AGCAGAAGAA GAACACCAGA GATGAATTTG AGGAGCGGGC CAAGGCGATC
    ATTGTGGAAT TTGCACAGCA GGGGCTGAAT GCAGCTTTGT TCTATGAGAA TAAGGATCCC
    CGCACTTTTG TGTCCTTGGT ACCTACCTCT GCACACACTG GCGATGGCAT GGGAAGTCTG
    ATCTGCCTTC TTGTTGAGTT GACTCAGACC ATGTTGAGTA AGAGGCTTGC CCAGTGTGAG
    GAGCTGAGAG CCCAGGTGAT GGAGGTTAAG GCTCTCCCGG GAATGGGCAC GACTATTGAT
    GTAATACTGA TCAACGGGCG TCTGAAGGAA GGAGACACGA TTATTGTTCC CGGAGTAGAA
    GGGCCTATCG TCACGCAGAT CCGAGGCCTC CTGTTACCTC CCCCTATGAA GGAGTTAAGA
    GTGAAGAACC AATATGAGAA GCATAAAGAG GTAGAAGCAG CTCAGGGGGT AAAGATTCTT
    GGAAAGGACC TGGAAAAAAC ATTGGCTGGT TTACCCCTCC TCGTGGCTTA TAAAGAAGAT
    GAAGTCCCAG TTCTTAAAGA TGAGCTGATC CACGAGTTAA AGCAGACACT GAACGCTATC
    AAATTAGAAG AGAAAGGCGT CTACGTCCAG GCCTCGACCC TGGGATCTCT GGAAGCTCTC
    CTTGAATTTC TGAAGACATC AGAAGTGCCC TACGCAGGGA TCAACATTGG GCCCGTCCAC
    AAAAAAGACG TCATGAAGGC TTCGGTGATG CTGGAACACG ACCCTCAGTA TGCAGTGATT
    TTGGCCTTCG ATGTGAGAAT TGAACGGGAT GCCCAAGAAA TGGCTGATAG TTTAGGAGTT
    AGAATTTTTA GTGCAGAAAT TATTTATCAT TTATTTGATG CCTTTACGAA ATACAGACAA
    GACTACAAGA AACAGAAACA AGAAGAATTT AAGCACATAG CAGTATTCCC CTGCAAGATA
    AAGATCCTCC CTCAGTTTAT CTTCAATTCT CGGGATCCGA TAGTGATGGG CGTGACTGTG
    GAAGCAGGGC AGGTGAAGCA GGGGACACCC ATGTGCGTGC CCAGCAAAAA TTTTGTTGAC
    ATTGGAATAG TAACAAGTAT TGAAGTAAAC CATAAACAAG TGGATGTTGC GAAAAAAGGC
    CAAGAAGTCT GTGTCAAAAT AGAACCCATC CCAGGTGAAT CTCCCAAAAT GTATGGACGC
    CATTTTGAAG CTACAGACAT TCTCGTCAGT AAGATCAGCC GGCAGTCCAT CGATGCTCTC
    AAAGACTGGT TCAGAGACGA GATGCAGAAG AGTGACTGGC AGCTTATTGT GGAGCTGAAG
    AAAGTGTTTG AAATCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23755
    Clone ID Related Accession (Same CDS sequence) XM_003586607.4 , XM_003582755.4
    Accession Version XM_003586607.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586607.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586655.1
    CDS211..3870
    Translation

    Target ORF information:

    RefSeq Version XM_003586607.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586607.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23755
    Clone ID Related Accession (Same CDS sequence) XM_003586607.4 , XM_003582755.4
    Accession Version XM_003582755.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582755.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582803.2
    CDS211..3870
    Translation

    Target ORF information:

    RefSeq Version XM_003582755.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 5B (EIF5B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582755.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAGA AGCAGAAAAA CAAGAACGAA GACAGTGCCA AGGATGACAT TGATCTTGAT 
    GCCTTGGCTG CAGAAATAGA AGGAGCTGGT GCTGCCAGGG AGCAAGAACC TCAAAAGGCA
    AAGGGGAAAA AGAAAAAGGA GAAAAAAAGA CAAGACTTTG ATGAAGATGA TATCCTGAAA
    GAACTGGAAG AATTGTCTTG GGAAGCTCAA GGCATCAAAG CTGACAGAGA GCCTGCTGCG
    GTCAAGCCAA CAGAAAATAA TGAAGATGAA CCCATCTCAA AACAAGATAA GAAAAAGAAA
    GGACAGAAAG GCAAAAAACA GAGTTTTGAA GATAATGATA GTGAAGAATT GGAAGAAAAA
    GATTCAAAGT CAAAAAAGAC TGCAAAACCC AAAGTGGAAA TGTACTCTGG GAGTGATGAT
    GATGATGATT TTAATAAACT ATCCAAAAAA GCAAAGGGGA AAGCTCAGAA ATCAAATAAG
    AAGCGTGATG CATCAGAAGA GGATGAGGAT AACAGTAAAA GAATTAAAGA GCGTATGAGA
    GCACATTCTT CTGGTGAAAG TGGTGATGAA TCAGATGAAT TTTTGCAATC CAGGAAAGGA
    CAGAAAAAAA ATCAGAAAAA CAAGCCAGGT CCTACTGTCG AGAGTGGAAA TGAAGATGAT
    GACTCGTCCT TCAAAATTAA GACAGTGGCT CAAAAGAAAG CAGAGAAGAA AGAGCGTGAG
    AGAAAAAAAC GGGATGAAGA AAAGGCAAAA CTTCGGAAGC TGAAAGAAAA AGAAGAGCTG
    GAGTCTGGTA AAAAGGATCT GGGTAAACCA AAAGAATCTC AGCGGAAACC TGAGGAGGAA
    GCTCTGAAAT CCAAAGTGAC ACTTGACTCT GGGGCAGCTC CTGCCTCTGA AGAGAAAGGA
    GAGGGTCCCA TAGGCGCAGA AGATGACAAT GAAGGAGACA AAAAGAAAAA AGATAAGAAG
    AAAAAGAAAG GAGAAAAGGA AGAAAAGGAG AAAGAAAAGA AGAAAGGACC TAGCAAAGCC
    ACAGTTAAGG CTATGCAAGA AGCTCTGGCT AAGCTTAAAG AAGAGGAGGA AAGACAGAAG
    AGAGAGGAGG AAGAACGGAT AAAACGGCTT GAAGAGTTAG AAGCCAAGCG TAAAGAAGAG
    GAAAGATTGG AACAAGAAAA AAGAGAAAGG AAAAAGCAAA AAGAAAAGGA AAGGAAAGAA
    CGCTTGAAAA AAGAGGGGAA ACTTTTAACT AAATCCCAGA GAGAAGCCAG AGCCAGAGCC
    GAAGCTACCC TTAAACTTCT ACAAGCTCAG GGTGTTGAAG TGCCATCAAA AGACTCTTTG
    CCAAAGAAGA GGCCAATTTA TGAAGATAAA AAGAAGAAAA ACNCACCGCA GCAGATGGAA
    AATAAAGTTT CTGAACCAGT GGAATTAAGT GCTGCTGCAG AAGTTGTGGA GCAAGGACTA
    CCCGAAAAAG AAGAGACACC GCCTCCTGTT GAACCAGAAG AGGAAGAAGA AACCGAGGAT
    GCTGGGTTGG ATGACTGGGA AGCTATGGCT AGCGATGAGG AGAGAGAGAC AGAAGAAAAC
    ACGGTGCATA TAGAAGTAAA AGAAAACCCT GAAGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAGGAAG AAGAGAGTGA AGACGAAGAG GAGGAAGAAG GAGACAGTGA AGGCAGTGAA
    GGTGAAGATG ATGATGAAAA GGTGTCAGAT GAGAAGGACG CTGGGAAAAC ATTGGATAAA
    AAGCCAAGCA AAGACGTGAG CTCAGAATCT GAGGATGACT CTGACGACGA TCGAACTAAA
    GAAGAACGGG CTTATGACAA AGCAAAACGG AGGATTGAGA AACGGCGACT TGAACACAGT
    AAAAATGTAA ATACAGAGAA GCTGAGAGCT CCTGTTATTT GTGTACTTGG ACATGTGGAT
    ACAGGGAAGA CAAAAATTCT AGATAAGCTC CGTCACACAC ATGTACAAGA TGGTGAAGCA
    GGTGGTATCA CACAGCAAAT TGGTGCCACC AATGTCCCTC TTGAAGCTAT TAATGAACAA
    ACTAAGATGA TTAAAAATTT TGATCGAGAG AACGTACGGA TTCCAGGAAT GCTGATTATT
    GACACTCCGG GGCATGAGTC TTTCAGTAAT CTGAGAAACA GAGGAAGCTC TCTTTGTGAC
    ATTGCCATTT TAGTTGTTGA TATTATGCAT GGTTTGGAGC CCCAGACAAT TGAATCTATC
    AACCTTTTGA AATCTAAAAA ATGTCCCTTC ATTGTTGCCC TCAATAAGAT TGATAGGTTG
    TATGATTGGA AAAAGAGCCC TGACTCTGAC GTGGCCGCTA CTCTGAAGAA GCAGAAGAAG
    AACACCAGAG ATGAATTTGA GGAGCGGGCC AAGGCGATCA TTGTGGAATT TGCACAGCAG
    GGGCTGAATG CAGCTTTGTT CTATGAGAAT AAGGATCCCC GCACTTTTGT GTCCTTGGTA
    CCTACCTCTG CACACACTGG CGATGGCATG GGAAGTCTGA TCTGCCTTCT TGTTGAGTTG
    ACTCAGACCA TGTTGAGTAA GAGGCTTGCC CAGTGTGAGG AGCTGAGAGC CCAGGTGATG
    GAGGTTAAGG CTCTCCCGGG AATGGGCACG ACTATTGATG TAATACTGAT CAACGGGCGT
    CTGAAGGAAG GAGACACGAT TATTGTTCCC GGAGTAGAAG GGCCTATCGT CACGCAGATC
    CGAGGCCTCC TGTTACCTCC CCCTATGAAG GAGTTAAGAG TGAAGAACCA ATATGAGAAG
    CATAAAGAGG TAGAAGCAGC TCAGGGGGTA AAGATTCTTG GAAAGGACCT GGAAAAAACA
    TTGGCTGGTT TACCCCTCCT CGTGGCTTAT AAAGAAGATG AAGTCCCAGT TCTTAAAGAT
    GAGCTGATCC ACGAGTTAAA GCAGACACTG AACGCTATCA AATTAGAAGA GAAAGGCGTC
    TACGTCCAGG CCTCGACCCT GGGATCTCTG GAAGCTCTCC TTGAATTTCT GAAGACATCA
    GAAGTGCCCT ACGCAGGGAT CAACATTGGG CCCGTCCACA AAAAAGACGT CATGAAGGCT
    TCGGTGATGC TGGAACACGA CCCTCAGTAT GCAGTGATTT TGGCCTTCGA TGTGAGAATT
    GAACGGGATG CCCAAGAAAT GGCTGATAGT TTAGGAGTTA GAATTTTTAG TGCAGAAATT
    ATTTATCATT TATTTGATGC CTTTACGAAA TACAGACAAG ACTACAAGAA ACAGAAACAA
    GAAGAATTTA AGCACATAGC AGTATTCCCC TGCAAGATAA AGATCCTCCC TCAGTTTATC
    TTCAATTCTC GGGATCCGAT AGTGATGGGC GTGACTGTGG AAGCAGGGCA GGTGAAGCAG
    GGGACACCCA TGTGCGTGCC CAGCAAAAAT TTTGTTGACA TTGGAATAGT AACAAGTATT
    GAAGTAAACC ATAAACAAGT GGATGTTGCG AAAAAAGGCC AAGAAGTCTG TGTCAAAATA
    GAACCCATCC CAGGTGAATC TCCCAAAATG TATGGACGCC ATTTTGAAGC TACAGACATT
    CTCGTCAGTA AGATCAGCCG GCAGTCCATC GATGCTCTCA AAGACTGGTT CAGAGACGAG
    ATGCAGAAGA GTGACTGGCA GCTTATTGTG GAGCTGAAGA AAGTGTTTGA AATCATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.