AAK1 cDNA ORF clone, Bos taurus(cattle)

The following AAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220861 XM_024999469.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220862 XM_024999470.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa220863 XM_024999471.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa220864 XM_024999472.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa220865 XM_024999473.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa220866 XM_024999474.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa220853 XM_024999451.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220854 XM_024999452.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220855 XM_024999453.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220856 XM_024999458.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220857 XM_024999461.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220858 XM_024999462.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220859 XM_024999463.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220860 XM_024999466.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa52204 XM_024999460.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91914 XM_015465403.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91914 XM_015473520.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91915 XM_015465404.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91915 XM_015473521.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa06949 XM_005193927.3
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa06949 XM_005212906.4
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa06950 XM_002691441.6
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa06950 XM_611658.8
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa52195 XM_024999450.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52195 XM_024999449.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52196 XM_010810143.3
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52196 XM_010816669.2
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52196 XM_015465397.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52196 XM_015473514.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52197 XM_010816670.2
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52197 XM_010810144.3
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52198 XM_024999454.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52199 XM_024999455.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52200 XM_024999456.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52201 XM_010816679.2
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52201 XM_010810148.2
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52202 XM_024999457.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52203 XM_024999459.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52205 XM_024999464.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52206 XM_024999465.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52207 XM_024999467.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52208 XM_024999468.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa91909 XM_015465398.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91909 XM_015473515.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91910 XM_015473516.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91910 XM_015465399.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91911 XM_015473517.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91911 XM_015465400.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91912 XM_015473518.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91912 XM_015465401.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91913 XM_015465402.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91913 XM_015473519.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa91916 XM_015465405.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91916 XM_015473522.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91917 XM_015465406.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91917 XM_015473523.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91918 XM_015473524.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91918 XM_015465407.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa91919 XM_015465408.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa91919 XM_015473525.1
Latest version!
Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OBa06949 XM_005212906.3 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa52197 XM_010810144.2 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52196 XM_010810143.2 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa06950 XM_002691441.5 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220861
    Clone ID Related Accession (Same CDS sequence) XM_024999469.1
    Accession Version XM_024999469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGCTGAAAAA
    AGGAAGGGTG GGCAGACTAT GGATTCTAGC CTTCCTCTTC TAAGTGTATC TGATCCTTTC
    ATTCCTCTTC AAGTACCTGA TGCACCAGAA AAACTAATTG AGGGACTCAA ATCTCCTGAC
    ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT ATGACAGATC CTTTTGGTAG CACTTCTGAT
    GCTGTAATTG AAAAAGCTGA TGTTGCTGTT GAGAGTCTGA TACCAGGACT GGAGCCCCCG
    GTGCCTCAGC GCCTTCCATC TCAGACGGAA TCTGTGACCT CGAACCGCAC AGATTCTCTC
    ACCGGGGAAG ATTCCCTGAT TGATTGCTCT CTGCTTTCTA ACCCCACTAC TGACCTTCTG
    GAAGAGTTTG CCCCCATAGC CATCTCTGCT CCGGCCCATA AAGCTGCGGA AGATAGTAAT
    CTCATCTCAG GTTTTGATGT CCCTGAGGGC TCGGACAAGG TGGCAGAAGA TGAGTTTGAC
    CCTATTCCTG TATTGATAAC CAAAAACCCA CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG
    AGCTCTGAGT CCAGTCTTCC CAACCTAGCC AGGTCTTTGC TGCTGGTGGA TCAGCTCATA
    GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855237.1
    CDS395..3163
    Translation

    Target ORF information:

    RefSeq Version XM_024999469.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGCTGAAAAA
    AGGAAGGGTG GGCAGACTAT GGATTCTAGC CTTCCTCTTC TAAGTGTATC TGATCCTTTC
    ATTCCTCTTC AAGTACCTGA TGCACCAGAA AAACTAATTG AGGGACTCAA ATCTCCTGAC
    ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT ATGACAGATC CTTTTGGTAG CACTTCTGAT
    GCTGTAATTG AAAAAGCTGA TGTTGCTGTT GAGAGTCTGA TACCAGGACT GGAGCCCCCG
    GTGCCTCAGC GCCTTCCATC TCAGACGGAA TCTGTGACCT CGAACCGCAC AGATTCTCTC
    ACCGGGGAAG ATTCCCTGAT TGATTGCTCT CTGCTTTCTA ACCCCACTAC TGACCTTCTG
    GAAGAGTTTG CCCCCATAGC CATCTCTGCT CCGGCCCATA AAGCTGCGGA AGATAGTAAT
    CTCATCTCAG GTTTTGATGT CCCTGAGGGC TCGGACAAGG TGGCAGAAGA TGAGTTTGAC
    CCTATTCCTG TATTGATAAC CAAAAACCCA CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG
    AGCTCTGAGT CCAGTCTTCC CAACCTAGCC AGGTCTTTGC TGCTGGTGGA TCAGCTCATA
    GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220862
    Clone ID Related Accession (Same CDS sequence) XM_024999470.1
    Accession Version XM_024999470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT
    AAGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855238.1
    CDS377..2965
    Translation

    Target ORF information:

    RefSeq Version XM_024999470.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT
    AAGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220863
    Clone ID Related Accession (Same CDS sequence) XM_024999471.1
    Accession Version XM_024999471.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTCTTGG TTCTAAGTAA CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855239.1
    CDS375..2771
    Translation

    Target ORF information:

    RefSeq Version XM_024999471.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999471.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTCTTGG TTCTAAGTAA CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220864
    Clone ID Related Accession (Same CDS sequence) XM_024999472.1
    Accession Version XM_024999472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855240.1
    CDS375..2765
    Translation

    Target ORF information:

    RefSeq Version XM_024999472.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220865
    Clone ID Related Accession (Same CDS sequence) XM_024999473.1
    Accession Version XM_024999473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTTATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855241.1
    CDS375..2756
    Translation

    Target ORF information:

    RefSeq Version XM_024999473.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTTATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220866
    Clone ID Related Accession (Same CDS sequence) XM_024999474.1
    Accession Version XM_024999474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGTC
    TTGGTTCTAA GTAACATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855242.1
    CDS374..2674
    Translation

    Target ORF information:

    RefSeq Version XM_024999474.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGTC
    TTGGTTCTAA GTAACATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220853
    Clone ID Related Accession (Same CDS sequence) XM_024999451.1
    Accession Version XM_024999451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGGGTGG GAACCTACCT AGGTCTGCAA CCACCACTCC TTCAGGCTCC
    CCTCGGGCCT CCCAGCAAAA TGTTTATAAT CCTTCAGAAG GTTCTACCTG GAATCCCTTT
    GATGACGACA ATTTCTCCAA ACTGACAGCT GAAGAACTGC TAAACAAGGA CTTTGCCAAG
    CTGGGGGAAG GCAAGTATCC TGAGAAGCTT GGAGGCTCTG CTGAGAGTTT GATCCCAGGC
    TTTCAACCAA CCCAAGGTGA TGCTTTTGCT GCTTCCTCAT TTTCTGCTGG AACTGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855219.1
    CDS374..4285
    Translation

    Target ORF information:

    RefSeq Version XM_024999451.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGGGTGG GAACCTACCT AGGTCTGCAA CCACCACTCC TTCAGGCTCC
    CCTCGGGCCT CCCAGCAAAA TGTTTATAAT CCTTCAGAAG GTTCTACCTG GAATCCCTTT
    GATGACGACA ATTTCTCCAA ACTGACAGCT GAAGAACTGC TAAACAAGGA CTTTGCCAAG
    CTGGGGGAAG GCAAGTATCC TGAGAAGCTT GGAGGCTCTG CTGAGAGTTT GATCCCAGGC
    TTTCAACCAA CCCAAGGTGA TGCTTTTGCT GCTTCCTCAT TTTCTGCTGG AACTGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220854
    Clone ID Related Accession (Same CDS sequence) XM_024999452.1
    Accession Version XM_024999452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCTGAT GCTGTAATTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855220.1
    CDS374..4192
    Translation

    Target ORF information:

    RefSeq Version XM_024999452.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCTGAT GCTGTAATTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220855
    Clone ID Related Accession (Same CDS sequence) XM_024999453.1
    Accession Version XM_024999453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855221.1
    CDS374..4186
    Translation

    Target ORF information:

    RefSeq Version XM_024999453.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220856
    Clone ID Related Accession (Same CDS sequence) XM_024999458.1
    Accession Version XM_024999458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAAGCTGAT
    GTTGCTGTTG AGAGTCTGAT ACCAGGACTG GAGCCCCCGG TGCCTCAGCG CCTTCCATCT
    CAGACGGAAT CTGTGACCTC GAACCGCACA GATTCTCTCA CCGGGGAAGA TTCCCTGATT
    GATTGCTCTC TGCTTTCTAA CCCCACTACT GACCTTCTGG AAGAGTTTGC CCCCATAGCC
    ATCTCTGCTC CGGCCCATAA AGCTGCGGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCAGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC
    TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT
    GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC
    AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC
    TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT
    CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG
    GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA
    GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC
    GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG
    CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG
    TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC
    ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC
    ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT
    CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA
    AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC
    CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA
    GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT
    GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT
    TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855226.1
    CDS374..4081
    Translation

    Target ORF information:

    RefSeq Version XM_024999458.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAAGCTGAT
    GTTGCTGTTG AGAGTCTGAT ACCAGGACTG GAGCCCCCGG TGCCTCAGCG CCTTCCATCT
    CAGACGGAAT CTGTGACCTC GAACCGCACA GATTCTCTCA CCGGGGAAGA TTCCCTGATT
    GATTGCTCTC TGCTTTCTAA CCCCACTACT GACCTTCTGG AAGAGTTTGC CCCCATAGCC
    ATCTCTGCTC CGGCCCATAA AGCTGCGGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCAGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC
    TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT
    GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC
    AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC
    TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT
    CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG
    GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA
    GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC
    GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG
    CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG
    TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC
    ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC
    ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT
    CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA
    AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC
    CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA
    GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT
    GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT
    TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220857
    Clone ID Related Accession (Same CDS sequence) XM_024999461.1
    Accession Version XM_024999461.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGGGTGGG
    AACCTACCTA GGTCTGCAAC CACCACTCCT TCAGGCTCCC CTCGGGCCTC CCAGCAAAAT
    GTTTATAATC CTTCAGAAGG TTCTACCTGG AATCCCTTTG ATGACGACAA TTTCTCCAAA
    CTGACAGCTG AAGAACTGCT AAACAAGGAC TTTGCCAAGC TGGGGGAAGG CAAGTATCCT
    GAGAAGCTTG GAGGCTCTGC TGAGAGTTTG ATCCCAGGCT TTCAACCAAC CCAAGGTGAT
    GCTTTTGCTG CTTCCTCATT TTCTGCTGGA ACTGCTGAAA AAAGGAAGGG TGGGCAGACT
    ATGGATTCTA GCCTTCCTCT TCTAAGTGTA TCTGATCCTT TCATTCCTCT TCAAGTACCT
    GATGCACCAG AAAAACTAAT TGAGGGACTC AAATCTCCTG ACACTTCTCT TCTGCTCCCT
    GACCTCTTGC CTATGACAGA TCCTTTTGGT AGCACTTCTG ATGCTGTAAT TGAAAAAGCT
    GATGTTGCTG TTGAGAGTCT GATACCAGGA CTGGAGCCCC CGGTGCCTCA GCGCCTTCCA
    TCTCAGACGG AATCTGTGAC CTCGAACCGC ACAGATTCTC TCACCGGGGA AGATTCCCTG
    ATTGATTGCT CTCTGCTTTC TAACCCCACT ACTGACCTTC TGGAAGAGTT TGCCCCCATA
    GCCATCTCTG CTCCGGCCCA TAAAGCTGCG GAAGATAGTA ATCTCATCTC AGGTTTTGAT
    GTCCCTGAGG GCTCGGACAA GGTGGCAGAA GATGAGTTTG ACCCTATTCC TGTATTGATA
    ACCAAAAACC CACAAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855229.1
    CDS376..4026
    Translation

    Target ORF information:

    RefSeq Version XM_024999461.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGGGTGGG
    AACCTACCTA GGTCTGCAAC CACCACTCCT TCAGGCTCCC CTCGGGCCTC CCAGCAAAAT
    GTTTATAATC CTTCAGAAGG TTCTACCTGG AATCCCTTTG ATGACGACAA TTTCTCCAAA
    CTGACAGCTG AAGAACTGCT AAACAAGGAC TTTGCCAAGC TGGGGGAAGG CAAGTATCCT
    GAGAAGCTTG GAGGCTCTGC TGAGAGTTTG ATCCCAGGCT TTCAACCAAC CCAAGGTGAT
    GCTTTTGCTG CTTCCTCATT TTCTGCTGGA ACTGCTGAAA AAAGGAAGGG TGGGCAGACT
    ATGGATTCTA GCCTTCCTCT TCTAAGTGTA TCTGATCCTT TCATTCCTCT TCAAGTACCT
    GATGCACCAG AAAAACTAAT TGAGGGACTC AAATCTCCTG ACACTTCTCT TCTGCTCCCT
    GACCTCTTGC CTATGACAGA TCCTTTTGGT AGCACTTCTG ATGCTGTAAT TGAAAAAGCT
    GATGTTGCTG TTGAGAGTCT GATACCAGGA CTGGAGCCCC CGGTGCCTCA GCGCCTTCCA
    TCTCAGACGG AATCTGTGAC CTCGAACCGC ACAGATTCTC TCACCGGGGA AGATTCCCTG
    ATTGATTGCT CTCTGCTTTC TAACCCCACT ACTGACCTTC TGGAAGAGTT TGCCCCCATA
    GCCATCTCTG CTCCGGCCCA TAAAGCTGCG GAAGATAGTA ATCTCATCTC AGGTTTTGAT
    GTCCCTGAGG GCTCGGACAA GGTGGCAGAA GATGAGTTTG ACCCTATTCC TGTATTGATA
    ACCAAAAACC CACAAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220858
    Clone ID Related Accession (Same CDS sequence) XM_024999462.1
    Accession Version XM_024999462.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855230.1
    CDS374..3979
    Translation

    Target ORF information:

    RefSeq Version XM_024999462.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220859
    Clone ID Related Accession (Same CDS sequence) XM_024999463.1
    Accession Version XM_024999463.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855231.1
    CDS374..3868
    Translation

    Target ORF information:

    RefSeq Version XM_024999463.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220860
    Clone ID Related Accession (Same CDS sequence) XM_024999466.1
    Accession Version XM_024999466.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855234.1
    CDS374..3757
    Translation

    Target ORF information:

    RefSeq Version XM_024999466.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52204
    Clone ID Related Accession (Same CDS sequence) XM_024999460.1
    Accession Version XM_024999460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGGGT
    GGGAACCTAC CTAGGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855228.1
    CDS376..4029
    Translation

    Target ORF information:

    RefSeq Version XM_024999460.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGGGT
    GGGAACCTAC CTAGGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91914
    Clone ID Related Accession (Same CDS sequence) XM_015465403.1 , XM_015473520.1
    Accession Version XM_015465403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT
    GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA
    GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA
    CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC
    TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC
    TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT
    CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA
    CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG
    ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG
    AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT
    GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG
    CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG
    GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG
    GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA
    GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG
    ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT
    CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC
    CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT
    CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG
    CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG
    CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT
    GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT
    TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG
    GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC
    CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC
    CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT
    GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC
    TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT
    CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT
    TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC
    ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC
    ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA
    CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320889.1
    CDS397..4032
    Translation

    Target ORF information:

    RefSeq Version XM_015465403.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT
    GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA
    GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA
    CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC
    TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC
    TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT
    CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA
    CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG
    ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG
    AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT
    GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG
    CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG
    GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG
    GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA
    GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG
    ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT
    CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC
    CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT
    CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG
    CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG
    CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT
    GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT
    TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG
    GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC
    CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC
    CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT
    GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC
    TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT
    CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT
    TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC
    ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC
    ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA
    CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91914
    Clone ID Related Accession (Same CDS sequence) XM_015465403.1 , XM_015473520.1
    Accession Version XM_015473520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT
    GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA
    GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA
    CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC
    TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC
    TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT
    CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA
    CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG
    ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG
    AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT
    GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG
    CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG
    GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG
    GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA
    GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG
    ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT
    CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC
    CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT
    CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG
    CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG
    CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT
    GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT
    TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG
    GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC
    CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC
    CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT
    GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC
    TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT
    CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT
    TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC
    ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC
    ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA
    CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329006.1
    CDS397..4032
    Translation

    Target ORF information:

    RefSeq Version XM_015473520.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC
    ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT
    GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC
    AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT
    GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA
    GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA
    CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC
    TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC
    TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT
    CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA
    CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG
    ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG
    AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT
    GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG
    CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG
    GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG
    GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA
    GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG
    ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT
    CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC
    CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT
    CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG
    CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG
    CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT
    GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT
    TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG
    GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC
    CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC
    CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT
    GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC
    TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT
    CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT
    TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC
    ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC
    ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA
    CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91915
    Clone ID Related Accession (Same CDS sequence) XM_015465404.1 , XM_015473521.1
    Accession Version XM_015465404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA
    ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA
    GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG
    CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT
    GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA
    TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT
    CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA
    ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA
    GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320890.1
    CDS397..4029
    Translation

    Target ORF information:

    RefSeq Version XM_015465404.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA
    ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA
    GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG
    CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT
    GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA
    TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT
    CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA
    ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA
    GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91915
    Clone ID Related Accession (Same CDS sequence) XM_015465404.1 , XM_015473521.1
    Accession Version XM_015473521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA
    ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA
    GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG
    CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT
    GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA
    TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT
    CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA
    ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA
    GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329007.1
    CDS397..4029
    Translation

    Target ORF information:

    RefSeq Version XM_015473521.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC
    CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG
    CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA
    TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA
    ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA
    GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG
    CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT
    GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA
    TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT
    CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA
    ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA
    GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT
    CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG
    ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT
    TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC
    CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC
    AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06949
    Clone ID Related Accession (Same CDS sequence) XM_005212906.3 , XM_005212906.4 , XM_005193927.3
    Accession Version XM_005212906.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212963.1
    CDS397..3273
    Translation

    Target ORF information:

    RefSeq Version XM_005212906.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212906.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06949
    Clone ID Related Accession (Same CDS sequence) XM_005212906.3 , XM_005212906.4 , XM_005193927.3
    Accession Version XM_005212906.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212963.1
    CDS377..3253
    Translation

    Target ORF information:

    RefSeq Version XM_005212906.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06949
    Clone ID Related Accession (Same CDS sequence) XM_005212906.3 , XM_005212906.4 , XM_005193927.3
    Accession Version XM_005193927.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193984.1
    CDS397..3273
    Translation

    Target ORF information:

    RefSeq Version XM_005193927.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193927.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06950
    Clone ID Related Accession (Same CDS sequence) XM_611658.8 , XM_002691441.5 , XM_002691441.6
    Accession Version XM_611658.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_611658.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_611658.6
    CDS396..3269
    Translation

    Target ORF information:

    RefSeq Version XM_611658.8
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_611658.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06950
    Clone ID Related Accession (Same CDS sequence) XM_611658.8 , XM_002691441.5 , XM_002691441.6
    Accession Version XM_002691441.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691441.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691487.2
    CDS396..3269
    Translation

    Target ORF information:

    RefSeq Version XM_002691441.5
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691441.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06950
    Clone ID Related Accession (Same CDS sequence) XM_611658.8 , XM_002691441.5 , XM_002691441.6
    Accession Version XM_002691441.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691441.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691487.2
    CDS376..3249
    Translation

    Target ORF information:

    RefSeq Version XM_002691441.6
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691441.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT
    CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52195
    Clone ID Related Accession (Same CDS sequence) XM_024999449.1 , XM_024999450.1
    Accession Version XM_024999449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855217.1
    CDS375..4289
    Translation

    Target ORF information:

    RefSeq Version XM_024999449.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52195
    Clone ID Related Accession (Same CDS sequence) XM_024999449.1 , XM_024999450.1
    Accession Version XM_024999450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855218.1
    CDS480..4394
    Translation

    Target ORF information:

    RefSeq Version XM_024999450.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52196
    Clone ID Related Accession (Same CDS sequence) XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
    Accession Version XM_010810143.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808445.1
    CDS374..4270
    Translation

    Target ORF information:

    RefSeq Version XM_010810143.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810143.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52196
    Clone ID Related Accession (Same CDS sequence) XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
    Accession Version XM_015473514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329000.1
    CDS493..4389
    Translation

    Target ORF information:

    RefSeq Version XM_015473514.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52196
    Clone ID Related Accession (Same CDS sequence) XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
    Accession Version XM_010816669.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814971.1
    CDS397..4293
    Translation

    Target ORF information:

    RefSeq Version XM_010816669.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816669.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52196
    Clone ID Related Accession (Same CDS sequence) XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
    Accession Version XM_015465397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320883.1
    CDS493..4389
    Translation

    Target ORF information:

    RefSeq Version XM_015465397.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52196
    Clone ID Related Accession (Same CDS sequence) XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
    Accession Version XM_010810143.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808445.1
    CDS397..4293
    Translation

    Target ORF information:

    RefSeq Version XM_010810143.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52197
    Clone ID Related Accession (Same CDS sequence) XM_010816670.2 , XM_010810144.2 , XM_010810144.3
    Accession Version XM_010816670.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814972.1
    CDS397..4290
    Translation

    Target ORF information:

    RefSeq Version XM_010816670.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52197
    Clone ID Related Accession (Same CDS sequence) XM_010816670.2 , XM_010810144.2 , XM_010810144.3
    Accession Version XM_010810144.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808446.1
    CDS397..4290
    Translation

    Target ORF information:

    RefSeq Version XM_010810144.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52197
    Clone ID Related Accession (Same CDS sequence) XM_010816670.2 , XM_010810144.2 , XM_010810144.3
    Accession Version XM_010810144.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808446.1
    CDS374..4267
    Translation

    Target ORF information:

    RefSeq Version XM_010810144.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810144.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG
    ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC
    CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC
    TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT
    CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA
    AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC
    AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT
    CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT
    GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG
    ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA
    CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC
    CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA
    GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT
    CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC
    CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC
    ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT
    GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG
    ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA
    GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG
    GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT
    TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG
    CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC
    AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT
    GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA
    ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG
    ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT
    TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG
    GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC
    GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG
    TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA
    GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT
    GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA
    CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG
    AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG
    ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT
    GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52198
    Clone ID Related Accession (Same CDS sequence) XM_024999454.1
    Accession Version XM_024999454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAACTAATT
    GAGGGACTCA AATCTCCTGA CACTTCTCTT CTGCTCCCTG ACCTCTTGCC TATGACAGAT
    CCTTTTGGTA GCACTTCTGA TGCTGTAATT GAAAAAGCTG ATGTTGCTGT TGAGAGTCTG
    ATACCAGGAC TGGAGCCCCC GGTGCCTCAG CGCCTTCCAT CTCAGACGGA ATCTGTGACC
    TCGAACCGCA CAGATTCTCT CACCGGGGAA GATTCCCTGA TTGATTGCTC TCTGCTTTCT
    AACCCCACTA CTGACCTTCT GGAAGAGTTT GCCCCCATAG CCATCTCTGC TCCGGCCCAT
    AAAGCTGCGG AAGATAGTAA TCTCATCTCA GGTTTTGATG TCCCTGAGGG CTCGGACAAG
    GTGGCAGAAG ATGAGTTTGA CCCTATTCCT GTATTGATAA CCAAAAACCC ACAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855222.1
    CDS375..4184
    Translation

    Target ORF information:

    RefSeq Version XM_024999454.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT
    CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAACTAATT
    GAGGGACTCA AATCTCCTGA CACTTCTCTT CTGCTCCCTG ACCTCTTGCC TATGACAGAT
    CCTTTTGGTA GCACTTCTGA TGCTGTAATT GAAAAAGCTG ATGTTGCTGT TGAGAGTCTG
    ATACCAGGAC TGGAGCCCCC GGTGCCTCAG CGCCTTCCAT CTCAGACGGA ATCTGTGACC
    TCGAACCGCA CAGATTCTCT CACCGGGGAA GATTCCCTGA TTGATTGCTC TCTGCTTTCT
    AACCCCACTA CTGACCTTCT GGAAGAGTTT GCCCCCATAG CCATCTCTGC TCCGGCCCAT
    AAAGCTGCGG AAGATAGTAA TCTCATCTCA GGTTTTGATG TCCCTGAGGG CTCGGACAAG
    GTGGCAGAAG ATGAGTTTGA CCCTATTCCT GTATTGATAA CCAAAAACCC ACAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52199
    Clone ID Related Accession (Same CDS sequence) XM_024999455.1
    Accession Version XM_024999455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855223.1
    CDS374..4177
    Translation

    Target ORF information:

    RefSeq Version XM_024999455.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52200
    Clone ID Related Accession (Same CDS sequence) XM_024999456.1
    Accession Version XM_024999456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT
    GCGGAAGATA GTAATCTCAT CTCAGGTTTT GATGTCCCTG AGGGCTCGGA CAAGGTGGCA
    GAAGATGAGT TTGACCCTAT TCCTGTATTG ATAACCAAAA ACCCACAAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855224.1
    CDS374..4177
    Translation

    Target ORF information:

    RefSeq Version XM_024999456.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT
    GCGGAAGATA GTAATCTCAT CTCAGGTTTT GATGTCCCTG AGGGCTCGGA CAAGGTGGCA
    GAAGATGAGT TTGACCCTAT TCCTGTATTG ATAACCAAAA ACCCACAAGG TCTCCAGAGA
    GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA
    GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG
    GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA
    GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT
    TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT
    ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG
    AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG
    TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC
    CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG
    AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC
    CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT
    CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT
    AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC
    TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA
    GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT
    TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC
    AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT
    GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG
    AAATCAGGCA AACAGAAACC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52201
    Clone ID Related Accession (Same CDS sequence) XM_010816679.2 , XM_010810148.2
    Accession Version XM_010816679.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814981.1
    CDS397..4188
    Translation

    Target ORF information:

    RefSeq Version XM_010816679.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816679.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52201
    Clone ID Related Accession (Same CDS sequence) XM_010816679.2 , XM_010810148.2
    Accession Version XM_010810148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808450.1
    CDS397..4188
    Translation

    Target ORF information:

    RefSeq Version XM_010810148.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA
    AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT
    TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT
    GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT
    GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC
    CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT
    CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT
    CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT
    AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT
    GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC
    TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA
    TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC
    TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC
    GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA
    GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT
    CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC
    GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC
    CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC
    AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT
    ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC
    GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC
    CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC
    CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA
    TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA
    GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT
    AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG
    CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT
    ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA
    CAGAAACCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52202
    Clone ID Related Accession (Same CDS sequence) XM_024999457.1
    Accession Version XM_024999457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855225.1
    CDS374..4090
    Translation

    Target ORF information:

    RefSeq Version XM_024999457.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT
    GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC
    CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC
    CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC
    ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC
    TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT
    CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG
    CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA
    AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC
    CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC
    ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG
    ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT
    CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA
    GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT
    AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC
    AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG
    CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG
    TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT
    GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52203
    Clone ID Related Accession (Same CDS sequence) XM_024999459.1
    Accession Version XM_024999459.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855227.1
    CDS374..4066
    Translation

    Target ORF information:

    RefSeq Version XM_024999459.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52205
    Clone ID Related Accession (Same CDS sequence) XM_024999464.1
    Accession Version XM_024999464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGGTCTCC AGAGAGAGCC CATTTCCTCT
    CCTGTAATAA CTGTAGAGCA CTTTAAAGAG TCGACGGGTG TTAAAGGCCT TCCCCTATAC
    CCGGACCCCT CCCGGGTACC TGGCACGAAA ACTCAGAACA GTTTGGAATC TGACTACTTG
    GCCCGAGATG GCCCTTCCAG CAACAGCTCG TTCCACAGCA GCGAAGAGGA GGGGACCGAC
    CTCGAGGGGG ACATGCTGGA CTGCAGTGGC TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA
    GAGGATGAGA GCTGCAAGCC TCCGCAGGGG AAGCTAGGAG GAGCTACTCC CTTCACTCAG
    CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC TCGCAAGGTG GGAGGAAGAA CCAATTCGGA
    GCCCTCATTC AGCCAATTGC AGATGGACTC GGTGAGCCAG ATGTGTTCGC CACTGCCCCC
    TTCCGGAGCT CAAGGATTCC CGCTGATGAC ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG
    GGCAACGTGG CCCCTTCCCA GCCAGAGGAG ACGGATGTGT TCTTGAGAGC TCCCTTTACC
    AAGAAGAAAG GCATGGAGGA GTCAACAGTT GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT
    CTCCTCAGTC AGACCGATGA CATCCCTCTG CTCCCGGGCC TCGATCGAGC CGTGTACCCG
    TCTGCCCGAG CTCAGTATTC CATGGCTGGC TTTGCCCAGC CGTCTAACCT CCCCTCCCAT
    CCTGTACAAG CAGCAGACCA TCTTGACAGC ATCTCTTCCA GGGGCTCCTC CCTGGAATCT
    GGGGCCCATC CTAATGACAG AAACAAAGGA CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC
    CCCGTGGCAG GCAAACCATT CCGCCCCCAG TCTTTATCCA AATATTCCCG TCACTATAGC
    CCGGAAGATG AGCCGAATCC AGAAGCCCAG CCCATCGCTG CCTACAAAAT TGTTTCGCAA
    ACCAATAAGC AGTCGATAGC TGGCTCCGTT TCCATCACAT CCCTTGCCTC CAGGACTACG
    GAGCTGCCAG CTGCTGATCC TTTTGCTTTA GCACCCTTTC CTTCGAAATC AGGCAAACAG
    AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855232.1
    CDS374..3862
    Translation

    Target ORF information:

    RefSeq Version XM_024999464.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGGTCTCC AGAGAGAGCC CATTTCCTCT
    CCTGTAATAA CTGTAGAGCA CTTTAAAGAG TCGACGGGTG TTAAAGGCCT TCCCCTATAC
    CCGGACCCCT CCCGGGTACC TGGCACGAAA ACTCAGAACA GTTTGGAATC TGACTACTTG
    GCCCGAGATG GCCCTTCCAG CAACAGCTCG TTCCACAGCA GCGAAGAGGA GGGGACCGAC
    CTCGAGGGGG ACATGCTGGA CTGCAGTGGC TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA
    GAGGATGAGA GCTGCAAGCC TCCGCAGGGG AAGCTAGGAG GAGCTACTCC CTTCACTCAG
    CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC TCGCAAGGTG GGAGGAAGAA CCAATTCGGA
    GCCCTCATTC AGCCAATTGC AGATGGACTC GGTGAGCCAG ATGTGTTCGC CACTGCCCCC
    TTCCGGAGCT CAAGGATTCC CGCTGATGAC ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG
    GGCAACGTGG CCCCTTCCCA GCCAGAGGAG ACGGATGTGT TCTTGAGAGC TCCCTTTACC
    AAGAAGAAAG GCATGGAGGA GTCAACAGTT GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT
    CTCCTCAGTC AGACCGATGA CATCCCTCTG CTCCCGGGCC TCGATCGAGC CGTGTACCCG
    TCTGCCCGAG CTCAGTATTC CATGGCTGGC TTTGCCCAGC CGTCTAACCT CCCCTCCCAT
    CCTGTACAAG CAGCAGACCA TCTTGACAGC ATCTCTTCCA GGGGCTCCTC CCTGGAATCT
    GGGGCCCATC CTAATGACAG AAACAAAGGA CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC
    CCCGTGGCAG GCAAACCATT CCGCCCCCAG TCTTTATCCA AATATTCCCG TCACTATAGC
    CCGGAAGATG AGCCGAATCC AGAAGCCCAG CCCATCGCTG CCTACAAAAT TGTTTCGCAA
    ACCAATAAGC AGTCGATAGC TGGCTCCGTT TCCATCACAT CCCTTGCCTC CAGGACTACG
    GAGCTGCCAG CTGCTGATCC TTTTGCTTTA GCACCCTTTC CTTCGAAATC AGGCAAACAG
    AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52206
    Clone ID Related Accession (Same CDS sequence) XM_024999465.1
    Accession Version XM_024999465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855233.1
    CDS374..3766
    Translation

    Target ORF information:

    RefSeq Version XM_024999465.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGGT
    CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG
    GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG
    AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC
    AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC
    CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA
    GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA
    GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG
    CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT
    GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT
    GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG
    AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG
    GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC
    CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT
    TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG
    CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA
    TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC
    GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC
    ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC
    TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52207
    Clone ID Related Accession (Same CDS sequence) XM_024999467.1
    Accession Version XM_024999467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC
    TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT
    GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC
    AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC
    TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT
    CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG
    GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA
    GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC
    GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG
    CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG
    TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC
    ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC
    ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT
    CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA
    AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC
    CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA
    GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT
    GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT
    TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855235.1
    CDS374..3661
    Translation

    Target ORF information:

    RefSeq Version XM_024999467.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC
    TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT
    GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC
    AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC
    TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT
    CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG
    GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA
    GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC
    GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG
    CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG
    TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC
    ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC
    ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT
    CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA
    AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC
    CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA
    GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT
    GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT
    TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52208
    Clone ID Related Accession (Same CDS sequence) XM_024999468.1
    Accession Version XM_024999468.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTGGGCACTC TAGAAACAGC
    AGTGGGAGCT CTGAGTCCAG TCTTCCCAAC CTAGCCAGGT CTTTGCTGCT GGTGGATCAG
    CTCATAGACC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855236.1
    CDS379..3273
    Translation

    Target ORF information:

    RefSeq Version XM_024999468.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC
    TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC
    TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC
    AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA
    GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT
    GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT
    CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT
    CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT
    TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTGGGCACTC TAGAAACAGC
    AGTGGGAGCT CTGAGTCCAG TCTTCCCAAC CTAGCCAGGT CTTTGCTGCT GGTGGATCAG
    CTCATAGACC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91909
    Clone ID Related Accession (Same CDS sequence) XM_015473515.1 , XM_015465398.1
    Accession Version XM_015473515.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329001.1
    CDS397..4191
    Translation

    Target ORF information:

    RefSeq Version XM_015473515.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91909
    Clone ID Related Accession (Same CDS sequence) XM_015473515.1 , XM_015465398.1
    Accession Version XM_015465398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320884.1
    CDS397..4191
    Translation

    Target ORF information:

    RefSeq Version XM_015465398.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG
    CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT
    TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC
    CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT
    AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG
    TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91910
    Clone ID Related Accession (Same CDS sequence) XM_015473516.1 , XM_015465399.1
    Accession Version XM_015473516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329002.1
    CDS397..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015473516.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91910
    Clone ID Related Accession (Same CDS sequence) XM_015473516.1 , XM_015465399.1
    Accession Version XM_015465399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320885.1
    CDS397..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015465399.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91911
    Clone ID Related Accession (Same CDS sequence) XM_015465400.1 , XM_015473517.1
    Accession Version XM_015465400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320886.1
    CDS397..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015465400.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91911
    Clone ID Related Accession (Same CDS sequence) XM_015465400.1 , XM_015473517.1
    Accession Version XM_015473517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329003.1
    CDS397..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015473517.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT
    GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG
    GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC
    CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC
    GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG
    GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC
    GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC
    CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC
    CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC
    AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG
    AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC
    CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT
    GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT
    GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG
    GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC
    GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG
    GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC
    AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG
    CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91912
    Clone ID Related Accession (Same CDS sequence) XM_015473518.1 , XM_015465401.1
    Accession Version XM_015473518.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329004.1
    CDS397..4095
    Translation

    Target ORF information:

    RefSeq Version XM_015473518.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473518.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91912
    Clone ID Related Accession (Same CDS sequence) XM_015473518.1 , XM_015465401.1
    Accession Version XM_015465401.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320887.1
    CDS397..4095
    Translation

    Target ORF information:

    RefSeq Version XM_015465401.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT
    GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT
    CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT
    CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG
    TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA
    ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG
    TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC
    TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG
    AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC
    TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC
    GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC
    ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG
    ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT
    GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG
    CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC
    TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC
    ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA
    CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG
    TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG
    CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT
    TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA
    GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91913
    Clone ID Related Accession (Same CDS sequence) XM_015473519.1 , XM_015465402.1
    Accession Version XM_015473519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329005.1
    CDS397..4071
    Translation

    Target ORF information:

    RefSeq Version XM_015473519.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91913
    Clone ID Related Accession (Same CDS sequence) XM_015473519.1 , XM_015465402.1
    Accession Version XM_015465402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320888.1
    CDS397..4071
    Translation

    Target ORF information:

    RefSeq Version XM_015465402.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91916
    Clone ID Related Accession (Same CDS sequence) XM_015465405.1 , XM_015473522.1
    Accession Version XM_015465405.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320891.1
    CDS397..3867
    Translation

    Target ORF information:

    RefSeq Version XM_015465405.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91916
    Clone ID Related Accession (Same CDS sequence) XM_015465405.1 , XM_015473522.1
    Accession Version XM_015473522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329008.1
    CDS397..3867
    Translation

    Target ORF information:

    RefSeq Version XM_015473522.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG
    CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA
    CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC
    AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG
    GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG
    CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG
    CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT
    GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT
    CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC
    CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG
    GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT
    GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT
    TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC
    CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC
    AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA
    TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT
    CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA
    GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT
    CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91917
    Clone ID Related Accession (Same CDS sequence) XM_015473523.1 , XM_015465406.1
    Accession Version XM_015473523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329009.1
    CDS396..3770
    Translation

    Target ORF information:

    RefSeq Version XM_015473523.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91917
    Clone ID Related Accession (Same CDS sequence) XM_015473523.1 , XM_015465406.1
    Accession Version XM_015465406.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320892.1
    CDS396..3770
    Translation

    Target ORF information:

    RefSeq Version XM_015465406.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465406.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT
    TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC
    CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC
    TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG
    ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA
    GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC
    ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA
    TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT
    GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC
    TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC
    TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG
    TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG
    TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC
    TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG
    GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC
    TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC
    TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT
    TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG
    ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC
    AAACAGAAAC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91918
    Clone ID Related Accession (Same CDS sequence) XM_015465407.1 , XM_015473524.1
    Accession Version XM_015465407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320893.1
    CDS396..3665
    Translation

    Target ORF information:

    RefSeq Version XM_015465407.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91918
    Clone ID Related Accession (Same CDS sequence) XM_015465407.1 , XM_015473524.1
    Accession Version XM_015473524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329010.1
    CDS396..3665
    Translation

    Target ORF information:

    RefSeq Version XM_015473524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC
    CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT
    GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC
    AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC
    AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT
    CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA
    GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT
    GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA
    GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC
    AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG
    TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC
    CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC
    CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG
    CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC
    AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC
    CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC
    AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT
    GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA
    TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT
    CCTTCGAAAT CAGGCAAACA GAAACCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91919
    Clone ID Related Accession (Same CDS sequence) XM_015473525.1 , XM_015465408.1
    Accession Version XM_015473525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329011.1
    CDS396..2966
    Translation

    Target ORF information:

    RefSeq Version XM_015473525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91919
    Clone ID Related Accession (Same CDS sequence) XM_015473525.1 , XM_015465408.1
    Accession Version XM_015465408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AP2-associated protein kinase 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320894.1
    CDS396..2966
    Translation

    Target ORF information:

    RefSeq Version XM_015465408.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
    TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT
    GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC
    GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC
    AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG
    CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG
    TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC
    CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT
    GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT
    GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC
    AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG
    TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC
    ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT
    TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC
    TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC
    AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC
    CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC
    AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT
    ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA
    GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG
    CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA
    ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG
    CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC
    ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG
    CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA
    CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA
    TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG
    CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG
    CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG
    CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT
    CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA
    TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA
    GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC
    AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG
    CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC
    TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG
    TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA
    GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG
    CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA
    GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG
    CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA
    AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.