AAK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAK1
Entrez Gene ID	532546
Full Name	AP2 associated kinase 1
General protein information	Preferred Names AP2 associated kinase 1 Names AP2-associated protein kinase 1 adaptor-associated kinase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015465400.1	XP_015320886.1	AP2-associated protein kinase 1 isoform X6
XM_015465405.1	XP_015320891.1	AP2-associated protein kinase 1 isoform X11
XM_015473517.1	XP_015329003.1	AP2-associated protein kinase 1 isoform X6
XM_015473525.1	XP_015329011.1	AP2-associated protein kinase 1 isoform X16
XM_010816670.2	XP_010814972.1	AP2-associated protein kinase 1 isoform X2
XM_024999451.1	XP_024855219.1	AP2-associated protein kinase 1 isoform X2
XM_010810143.3	XP_010808445.1	AP2-associated protein kinase 1 isoform X3
XM_024999455.1	XP_024855223.1	AP2-associated protein kinase 1 isoform X8
XM_024999465.1	XP_024855233.1	AP2-associated protein kinase 1 isoform X18
XM_024999467.1	XP_024855235.1	AP2-associated protein kinase 1 isoform X20
XM_024999468.1	XP_024855236.1	AP2-associated protein kinase 1 isoform X21
XM_015473514.1	XP_015329000.1	AP2-associated protein kinase 1 isoform X1
XM_611658.8	XP_611658.6	AP2-associated protein kinase 1 isoform X15
XM_010816669.2	XP_010814971.1	AP2-associated protein kinase 1 isoform X1
XM_015465404.1	XP_015320890.1	AP2-associated protein kinase 1 isoform X10
XM_015473523.1	XP_015329009.1	AP2-associated protein kinase 1 isoform X12
XM_024999449.1	XP_024855217.1	AP2-associated protein kinase 1 isoform X1
XM_024999453.1	XP_024855221.1	AP2-associated protein kinase 1 isoform X6
XM_024999454.1	XP_024855222.1	AP2-associated protein kinase 1 isoform X7
XM_024999457.1	XP_024855225.1	AP2-associated protein kinase 1 isoform X10
XM_024999458.1	XP_024855226.1	AP2-associated protein kinase 1 isoform X11
XM_024999459.1	XP_024855227.1	AP2-associated protein kinase 1 isoform X12
XM_024999466.1	XP_024855234.1	AP2-associated protein kinase 1 isoform X19
XM_024999472.1	XP_024855240.1	AP2-associated protein kinase 1 isoform X27
XM_015465407.1	XP_015320893.1	AP2-associated protein kinase 1 isoform X13
XM_015473522.1	XP_015329008.1	AP2-associated protein kinase 1 isoform X11
XM_015473524.1	XP_015329010.1	AP2-associated protein kinase 1 isoform X13
XM_005212906.3	XP_005212963.1	AP2-associated protein kinase 1 isoform X14
XM_015473516.1	XP_015329002.1	AP2-associated protein kinase 1 isoform X5
XM_015473518.1	XP_015329004.1	AP2-associated protein kinase 1 isoform X7
XM_024999456.1	XP_024855224.1	AP2-associated protein kinase 1 isoform X9
XM_024999462.1	XP_024855230.1	AP2-associated protein kinase 1 isoform X15
XM_005212906.4	XP_005212963.1	AP2-associated protein kinase 1 isoform X22
XM_024999469.1	XP_024855237.1	AP2-associated protein kinase 1 isoform X24
XM_002691441.5	XP_002691487.2	AP2-associated protein kinase 1 isoform X15
XM_015473515.1	XP_015329001.1	AP2-associated protein kinase 1 isoform X3
XM_010810144.2	XP_010808446.1	AP2-associated protein kinase 1 isoform X2
XM_015465401.1	XP_015320887.1	AP2-associated protein kinase 1 isoform X7
XM_015465397.1	XP_015320883.1	AP2-associated protein kinase 1 isoform X1
XM_024999450.1	XP_024855218.1	AP2-associated protein kinase 1 isoform X1
XM_010810144.3	XP_010808446.1	AP2-associated protein kinase 1 isoform X4
XM_024999452.1	XP_024855220.1	AP2-associated protein kinase 1 isoform X5
XM_024999460.1	XP_024855228.1	AP2-associated protein kinase 1 isoform X13
XM_024999464.1	XP_024855232.1	AP2-associated protein kinase 1 isoform X17
XM_024999471.1	XP_024855239.1	AP2-associated protein kinase 1 isoform X26
XM_024999474.1	XP_024855242.1	AP2-associated protein kinase 1 isoform X29
XM_015473519.1	XP_015329005.1	AP2-associated protein kinase 1 isoform X8
XM_010816679.2	XP_010814981.1	AP2-associated protein kinase 1 isoform X4
XM_015465403.1	XP_015320889.1	AP2-associated protein kinase 1 isoform X9
XM_015473520.1	XP_015329006.1	AP2-associated protein kinase 1 isoform X9
XM_015473521.1	XP_015329007.1	AP2-associated protein kinase 1 isoform X10
XM_010810143.2	XP_010808445.1	AP2-associated protein kinase 1 isoform X1
XM_010810148.2	XP_010808450.1	AP2-associated protein kinase 1 isoform X4
XM_015465398.1	XP_015320884.1	AP2-associated protein kinase 1 isoform X3
XM_015465399.1	XP_015320885.1	AP2-associated protein kinase 1 isoform X5
XM_015465402.1	XP_015320888.1	AP2-associated protein kinase 1 isoform X8
XM_015465406.1	XP_015320892.1	AP2-associated protein kinase 1 isoform X12
XM_005193927.3	XP_005193984.1	AP2-associated protein kinase 1 isoform X14
XM_015465408.1	XP_015320894.1	AP2-associated protein kinase 1 isoform X16
XM_024999461.1	XP_024855229.1	AP2-associated protein kinase 1 isoform X14
XM_024999463.1	XP_024855231.1	AP2-associated protein kinase 1 isoform X16
XM_002691441.6	XP_002691487.2	AP2-associated protein kinase 1 isoform X23
XM_024999470.1	XP_024855238.1	AP2-associated protein kinase 1 isoform X25
XM_024999473.1	XP_024855241.1	AP2-associated protein kinase 1 isoform X28

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AAK1	XP_005212959.1
	LOC701067	XP_002799330.1

Gene Ontology

The following AAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220861	XM_024999469.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa220862	XM_024999470.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa220863	XM_024999471.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220864	XM_024999472.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220865	XM_024999473.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220866	XM_024999474.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa220853	XM_024999451.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220854	XM_024999452.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220855	XM_024999453.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220856	XM_024999458.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220857	XM_024999461.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220858	XM_024999462.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220859	XM_024999463.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220860	XM_024999466.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa52204	XM_024999460.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91914	XM_015465403.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91914	XM_015473520.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91915	XM_015465404.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa91915	XM_015473521.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa06949	XM_005193927.3 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa06949	XM_005212906.4 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa06950	XM_002691441.6 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa06950	XM_611658.8 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa52195	XM_024999450.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52195	XM_024999449.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52196	XM_010810143.3 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52196	XM_010816669.2 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52196	XM_015465397.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52196	XM_015473514.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52197	XM_010816670.2 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52197	XM_010810144.3 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52198	XM_024999454.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52199	XM_024999455.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52200	XM_024999456.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52201	XM_010816679.2 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52201	XM_010810148.2 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52202	XM_024999457.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52203	XM_024999459.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52205	XM_024999464.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52206	XM_024999465.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52207	XM_024999467.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa52208	XM_024999468.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa91909	XM_015465398.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91909	XM_015473515.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91910	XM_015473516.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91910	XM_015465399.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91911	XM_015473517.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91911	XM_015465400.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91912	XM_015473518.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91912	XM_015465401.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91913	XM_015465402.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91913	XM_015473519.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa91916	XM_015465405.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91916	XM_015473522.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91917	XM_015465406.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91917	XM_015473523.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91918	XM_015473524.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91918	XM_015465407.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa91919	XM_015465408.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa91919	XM_015473525.1 Latest version!	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa06949	XM_005212906.3	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa52197	XM_010810144.2	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52196	XM_010810143.2	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa06950	XM_002691441.5	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAK1 ( XM_024999469.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855237.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X25, mRNA.

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CloneID	OBa220861
Clone ID Related Accession (Same CDS sequence)	XM_024999469.1
Accession Version	XM_024999469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGCTGAAAAA 
AGGAAGGGTG GGCAGACTAT GGATTCTAGC CTTCCTCTTC TAAGTGTATC TGATCCTTTC 
ATTCCTCTTC AAGTACCTGA TGCACCAGAA AAACTAATTG AGGGACTCAA ATCTCCTGAC 
ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT ATGACAGATC CTTTTGGTAG CACTTCTGAT 
GCTGTAATTG AAAAAGCTGA TGTTGCTGTT GAGAGTCTGA TACCAGGACT GGAGCCCCCG 
GTGCCTCAGC GCCTTCCATC TCAGACGGAA TCTGTGACCT CGAACCGCAC AGATTCTCTC 
ACCGGGGAAG ATTCCCTGAT TGATTGCTCT CTGCTTTCTA ACCCCACTAC TGACCTTCTG 
GAAGAGTTTG CCCCCATAGC CATCTCTGCT CCGGCCCATA AAGCTGCGGA AGATAGTAAT 
CTCATCTCAG GTTTTGATGT CCCTGAGGGC TCGGACAAGG TGGCAGAAGA TGAGTTTGAC 
CCTATTCCTG TATTGATAAC CAAAAACCCA CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG 
AGCTCTGAGT CCAGTCTTCC CAACCTAGCC AGGTCTTTGC TGCTGGTGGA TCAGCTCATA 
GACCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855237.1
CDS	395..3163
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_024999469.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999469.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGCTGAAAAA 
AGGAAGGGTG GGCAGACTAT GGATTCTAGC CTTCCTCTTC TAAGTGTATC TGATCCTTTC 
ATTCCTCTTC AAGTACCTGA TGCACCAGAA AAACTAATTG AGGGACTCAA ATCTCCTGAC 
ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT ATGACAGATC CTTTTGGTAG CACTTCTGAT 
GCTGTAATTG AAAAAGCTGA TGTTGCTGTT GAGAGTCTGA TACCAGGACT GGAGCCCCCG 
GTGCCTCAGC GCCTTCCATC TCAGACGGAA TCTGTGACCT CGAACCGCAC AGATTCTCTC 
ACCGGGGAAG ATTCCCTGAT TGATTGCTCT CTGCTTTCTA ACCCCACTAC TGACCTTCTG 
GAAGAGTTTG CCCCCATAGC CATCTCTGCT CCGGCCCATA AAGCTGCGGA AGATAGTAAT 
CTCATCTCAG GTTTTGATGT CCCTGAGGGC TCGGACAAGG TGGCAGAAGA TGAGTTTGAC 
CCTATTCCTG TATTGATAAC CAAAAACCCA CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG 
AGCTCTGAGT CCAGTCTTCC CAACCTAGCC AGGTCTTTGC TGCTGGTGGA TCAGCTCATA 
GACCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999470.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855238.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OBa220862
Clone ID Related Accession (Same CDS sequence)	XM_024999470.1
Accession Version	XM_024999470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT 
AAGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855238.1
CDS	377..2965
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAKT

Target ORF information:

RefSeq Version	XM_024999470.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999470.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT 
AAGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999471.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855239.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa220863
Clone ID Related Accession (Same CDS sequence)	XM_024999471.1
Accession Version	XM_024999471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTCTTGG TTCTAAGTAA CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855239.1
CDS	375..2771
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPVLVLSNI

Target ORF information:

RefSeq Version	XM_024999471.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999471.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTCTTGG TTCTAAGTAA CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999472.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855240.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OBa220864
Clone ID Related Accession (Same CDS sequence)	XM_024999472.1
Accession Version	XM_024999472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855240.1
CDS	375..2765
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAKT

Target ORF information:

RefSeq Version	XM_024999472.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999472.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999473.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855241.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OBa220865
Clone ID Related Accession (Same CDS sequence)	XM_024999473.1
Accession Version	XM_024999473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTTATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855241.1
CDS	375..2756
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPVI

Target ORF information:

RefSeq Version	XM_024999473.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999473.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGTTATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999474.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855242.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OBa220866
Clone ID Related Accession (Same CDS sequence)	XM_024999474.1
Accession Version	XM_024999474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGTC 
TTGGTTCTAA GTAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855242.1
CDS	374..2674
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTVLVLSNI

Target ORF information:

RefSeq Version	XM_024999474.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999474.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGTC 
TTGGTTCTAA GTAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999451.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855219.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220853
Clone ID Related Accession (Same CDS sequence)	XM_024999451.1
Accession Version	XM_024999451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGGGTGG GAACCTACCT AGGTCTGCAA CCACCACTCC TTCAGGCTCC 
CCTCGGGCCT CCCAGCAAAA TGTTTATAAT CCTTCAGAAG GTTCTACCTG GAATCCCTTT 
GATGACGACA ATTTCTCCAA ACTGACAGCT GAAGAACTGC TAAACAAGGA CTTTGCCAAG 
CTGGGGGAAG GCAAGTATCC TGAGAAGCTT GGAGGCTCTG CTGAGAGTTT GATCCCAGGC 
TTTCAACCAA CCCAAGGTGA TGCTTTTGCT GCTTCCTCAT TTTCTGCTGG AACTGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855219.1
CDS	374..4285
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999451.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999451.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGGGTGG GAACCTACCT AGGTCTGCAA CCACCACTCC TTCAGGCTCC 
CCTCGGGCCT CCCAGCAAAA TGTTTATAAT CCTTCAGAAG GTTCTACCTG GAATCCCTTT 
GATGACGACA ATTTCTCCAA ACTGACAGCT GAAGAACTGC TAAACAAGGA CTTTGCCAAG 
CTGGGGGAAG GCAAGTATCC TGAGAAGCTT GGAGGCTCTG CTGAGAGTTT GATCCCAGGC 
TTTCAACCAA CCCAAGGTGA TGCTTTTGCT GCTTCCTCAT TTTCTGCTGG AACTGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999452.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855220.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa220854
Clone ID Related Accession (Same CDS sequence)	XM_024999452.1
Accession Version	XM_024999452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT 
ATGACAGATC CTTTTGGTAG CACTTCTGAT GCTGTAATTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855220.1
CDS	374..4192
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999452.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999452.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT 
ATGACAGATC CTTTTGGTAG CACTTCTGAT GCTGTAATTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999453.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855221.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa220855
Clone ID Related Accession (Same CDS sequence)	XM_024999453.1
Accession Version	XM_024999453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855221.1
CDS	374..4186
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999453.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999453.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999458.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855226.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

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CloneID	OBa220856
Clone ID Related Accession (Same CDS sequence)	XM_024999458.1
Accession Version	XM_024999458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAAGCTGAT 
GTTGCTGTTG AGAGTCTGAT ACCAGGACTG GAGCCCCCGG TGCCTCAGCG CCTTCCATCT 
CAGACGGAAT CTGTGACCTC GAACCGCACA GATTCTCTCA CCGGGGAAGA TTCCCTGATT 
GATTGCTCTC TGCTTTCTAA CCCCACTACT GACCTTCTGG AAGAGTTTGC CCCCATAGCC 
ATCTCTGCTC CGGCCCATAA AGCTGCGGAA GATAGTAATC TCATCTCAGG TTTTGATGTC 
CCTGAGGGCT CGGACAAGGT GGCAGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC 
AAAAACCCAC AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC 
TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT 
GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC 
AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC 
TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT 
CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG 
GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA 
GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC 
GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG 
CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG 
TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC 
ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC 
ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT 
CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA 
AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC 
CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA 
GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT 
GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT 
TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855226.1
CDS	374..4081
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999458.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999458.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAAGCTGAT 
GTTGCTGTTG AGAGTCTGAT ACCAGGACTG GAGCCCCCGG TGCCTCAGCG CCTTCCATCT 
CAGACGGAAT CTGTGACCTC GAACCGCACA GATTCTCTCA CCGGGGAAGA TTCCCTGATT 
GATTGCTCTC TGCTTTCTAA CCCCACTACT GACCTTCTGG AAGAGTTTGC CCCCATAGCC 
ATCTCTGCTC CGGCCCATAA AGCTGCGGAA GATAGTAATC TCATCTCAGG TTTTGATGTC 
CCTGAGGGCT CGGACAAGGT GGCAGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC 
AAAAACCCAC AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC 
TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT 
GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC 
AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC 
TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT 
CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG 
GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA 
GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC 
GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG 
CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG 
TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC 
ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC 
ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT 
CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA 
AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC 
CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA 
GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT 
GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT 
TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999461.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855229.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

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CloneID	OBa220857
Clone ID Related Accession (Same CDS sequence)	XM_024999461.1
Accession Version	XM_024999461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGGGTGGG 
AACCTACCTA GGTCTGCAAC CACCACTCCT TCAGGCTCCC CTCGGGCCTC CCAGCAAAAT 
GTTTATAATC CTTCAGAAGG TTCTACCTGG AATCCCTTTG ATGACGACAA TTTCTCCAAA 
CTGACAGCTG AAGAACTGCT AAACAAGGAC TTTGCCAAGC TGGGGGAAGG CAAGTATCCT 
GAGAAGCTTG GAGGCTCTGC TGAGAGTTTG ATCCCAGGCT TTCAACCAAC CCAAGGTGAT 
GCTTTTGCTG CTTCCTCATT TTCTGCTGGA ACTGCTGAAA AAAGGAAGGG TGGGCAGACT 
ATGGATTCTA GCCTTCCTCT TCTAAGTGTA TCTGATCCTT TCATTCCTCT TCAAGTACCT 
GATGCACCAG AAAAACTAAT TGAGGGACTC AAATCTCCTG ACACTTCTCT TCTGCTCCCT 
GACCTCTTGC CTATGACAGA TCCTTTTGGT AGCACTTCTG ATGCTGTAAT TGAAAAAGCT 
GATGTTGCTG TTGAGAGTCT GATACCAGGA CTGGAGCCCC CGGTGCCTCA GCGCCTTCCA 
TCTCAGACGG AATCTGTGAC CTCGAACCGC ACAGATTCTC TCACCGGGGA AGATTCCCTG 
ATTGATTGCT CTCTGCTTTC TAACCCCACT ACTGACCTTC TGGAAGAGTT TGCCCCCATA 
GCCATCTCTG CTCCGGCCCA TAAAGCTGCG GAAGATAGTA ATCTCATCTC AGGTTTTGAT 
GTCCCTGAGG GCTCGGACAA GGTGGCAGAA GATGAGTTTG ACCCTATTCC TGTATTGATA 
ACCAAAAACC CACAAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855229.1
CDS	376..4026
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999461.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999461.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGGGTGGG 
AACCTACCTA GGTCTGCAAC CACCACTCCT TCAGGCTCCC CTCGGGCCTC CCAGCAAAAT 
GTTTATAATC CTTCAGAAGG TTCTACCTGG AATCCCTTTG ATGACGACAA TTTCTCCAAA 
CTGACAGCTG AAGAACTGCT AAACAAGGAC TTTGCCAAGC TGGGGGAAGG CAAGTATCCT 
GAGAAGCTTG GAGGCTCTGC TGAGAGTTTG ATCCCAGGCT TTCAACCAAC CCAAGGTGAT 
GCTTTTGCTG CTTCCTCATT TTCTGCTGGA ACTGCTGAAA AAAGGAAGGG TGGGCAGACT 
ATGGATTCTA GCCTTCCTCT TCTAAGTGTA TCTGATCCTT TCATTCCTCT TCAAGTACCT 
GATGCACCAG AAAAACTAAT TGAGGGACTC AAATCTCCTG ACACTTCTCT TCTGCTCCCT 
GACCTCTTGC CTATGACAGA TCCTTTTGGT AGCACTTCTG ATGCTGTAAT TGAAAAAGCT 
GATGTTGCTG TTGAGAGTCT GATACCAGGA CTGGAGCCCC CGGTGCCTCA GCGCCTTCCA 
TCTCAGACGG AATCTGTGAC CTCGAACCGC ACAGATTCTC TCACCGGGGA AGATTCCCTG 
ATTGATTGCT CTCTGCTTTC TAACCCCACT ACTGACCTTC TGGAAGAGTT TGCCCCCATA 
GCCATCTCTG CTCCGGCCCA TAAAGCTGCG GAAGATAGTA ATCTCATCTC AGGTTTTGAT 
GTCCCTGAGG GCTCGGACAA GGTGGCAGAA GATGAGTTTG ACCCTATTCC TGTATTGATA 
ACCAAAAACC CACAAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999462.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855230.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa220858
Clone ID Related Accession (Same CDS sequence)	XM_024999462.1
Accession Version	XM_024999462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855230.1
CDS	374..3979
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999462.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999462.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999463.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855231.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa220859
Clone ID Related Accession (Same CDS sequence)	XM_024999463.1
Accession Version	XM_024999463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855231.1
CDS	374..3868
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999463.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999463.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999466.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855234.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa220860
Clone ID Related Accession (Same CDS sequence)	XM_024999466.1
Accession Version	XM_024999466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855234.1
CDS	374..3757
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999466.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999466.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999460.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855228.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

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CloneID	OBa52204
Clone ID Related Accession (Same CDS sequence)	XM_024999460.1
Accession Version	XM_024999460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGGGT 
GGGAACCTAC CTAGGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855228.1
CDS	376..4029
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999460.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999460.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGGGT 
GGGAACCTAC CTAGGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465403.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320889.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa91914
Clone ID Related Accession (Same CDS sequence)	XM_015465403.1 , XM_015473520.1
Accession Version	XM_015465403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320889.1
CDS	397..4032
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465403.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465403.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473520.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329006.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa91914
Clone ID Related Accession (Same CDS sequence)	XM_015465403.1 , XM_015473520.1
Accession Version	XM_015473520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329006.1
CDS	397..4032
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473520.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473520.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTTCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGAGTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCC GAGGTCACTC CTGCGCAGGC CAAGGCCTCG 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACG 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGGGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465404.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320890.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

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CloneID	OBa91915
Clone ID Related Accession (Same CDS sequence)	XM_015465404.1 , XM_015473521.1
Accession Version	XM_015465404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320890.1
CDS	397..4029
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465404.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465404.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473521.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329007.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

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CloneID	OBa91915
Clone ID Related Accession (Same CDS sequence)	XM_015465404.1 , XM_015473521.1
Accession Version	XM_015473521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329007.1
CDS	397..4029
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473521.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473521.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT TCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_005212906.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212963.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa06949
Clone ID Related Accession (Same CDS sequence)	XM_005212906.3 , XM_005212906.4 , XM_005193927.3
Accession Version	XM_005212906.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212963.1
CDS	397..3273
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_005212906.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212906.3

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_005212906.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212963.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OBa06949
Clone ID Related Accession (Same CDS sequence)	XM_005212906.3 , XM_005212906.4 , XM_005193927.3
Accession Version	XM_005212906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212963.1
CDS	377..3253
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_005212906.4
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212906.4

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_005193927.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005193984.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa06949
Clone ID Related Accession (Same CDS sequence)	XM_005212906.3 , XM_005212906.4 , XM_005193927.3
Accession Version	XM_005193927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005193984.1
CDS	397..3273
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_005193927.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005193927.3

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_611658.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_611658.6 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa06950
Clone ID Related Accession (Same CDS sequence)	XM_611658.8 , XM_002691441.5 , XM_002691441.6
Accession Version	XM_611658.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_611658.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_611658.6
CDS	396..3269
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_611658.8
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_611658.8

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_002691441.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691487.2 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa06950
Clone ID Related Accession (Same CDS sequence)	XM_611658.8 , XM_002691441.5 , XM_002691441.6
Accession Version	XM_002691441.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691441.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691487.2
CDS	396..3269
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_002691441.5
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691441.5

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_002691441.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691487.2 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa06950
Clone ID Related Accession (Same CDS sequence)	XM_611658.8 , XM_002691441.5 , XM_002691441.6
Accession Version	XM_002691441.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691441.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691487.2
CDS	376..3249
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_002691441.6
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691441.6

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999449.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855217.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52195
Clone ID Related Accession (Same CDS sequence)	XM_024999449.1 , XM_024999450.1
Accession Version	XM_024999449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855217.1
CDS	375..4289
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999449.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999449.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999450.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855218.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

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CloneID	OBa52195
Clone ID Related Accession (Same CDS sequence)	XM_024999449.1 , XM_024999450.1
Accession Version	XM_024999450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855218.1
CDS	480..4394
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999450.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999450.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010810143.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808445.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa52196
Clone ID Related Accession (Same CDS sequence)	XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
Accession Version	XM_010810143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808445.1
CDS	374..4270
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010810143.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810143.3

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473514.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329000.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52196
Clone ID Related Accession (Same CDS sequence)	XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
Accession Version	XM_015473514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329000.1
CDS	493..4389
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473514.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473514.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010816669.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814971.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

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CloneID	OBa52196
Clone ID Related Accession (Same CDS sequence)	XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
Accession Version	XM_010816669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814971.1
CDS	397..4293
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010816669.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816669.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320883.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52196
Clone ID Related Accession (Same CDS sequence)	XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
Accession Version	XM_015465397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320883.1
CDS	493..4389
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465397.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010810143.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808445.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52196
Clone ID Related Accession (Same CDS sequence)	XM_010810143.3 , XM_015473514.1 , XM_010816669.2 , XM_015465397.1 , XM_010810143.2
Accession Version	XM_010810143.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808445.1
CDS	397..4293
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010810143.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810143.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010816670.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814972.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

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CloneID	OBa52197
Clone ID Related Accession (Same CDS sequence)	XM_010816670.2 , XM_010810144.2 , XM_010810144.3
Accession Version	XM_010816670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814972.1
CDS	397..4290
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010816670.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816670.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010810144.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808446.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52197
Clone ID Related Accession (Same CDS sequence)	XM_010816670.2 , XM_010810144.2 , XM_010810144.3
Accession Version	XM_010810144.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808446.1
CDS	397..4290
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010810144.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810144.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010810144.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808446.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa52197
Clone ID Related Accession (Same CDS sequence)	XM_010816670.2 , XM_010810144.2 , XM_010810144.3
Accession Version	XM_010810144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808446.1
CDS	374..4267
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010810144.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810144.3

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TTCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGAGTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTGCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCCGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACGGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GGGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999454.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855222.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

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CloneID	OBa52198
Clone ID Related Accession (Same CDS sequence)	XM_024999454.1
Accession Version	XM_024999454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAACTAATT 
GAGGGACTCA AATCTCCTGA CACTTCTCTT CTGCTCCCTG ACCTCTTGCC TATGACAGAT 
CCTTTTGGTA GCACTTCTGA TGCTGTAATT GAAAAAGCTG ATGTTGCTGT TGAGAGTCTG 
ATACCAGGAC TGGAGCCCCC GGTGCCTCAG CGCCTTCCAT CTCAGACGGA ATCTGTGACC 
TCGAACCGCA CAGATTCTCT CACCGGGGAA GATTCCCTGA TTGATTGCTC TCTGCTTTCT 
AACCCCACTA CTGACCTTCT GGAAGAGTTT GCCCCCATAG CCATCTCTGC TCCGGCCCAT 
AAAGCTGCGG AAGATAGTAA TCTCATCTCA GGTTTTGATG TCCCTGAGGG CTCGGACAAG 
GTGGCAGAAG ATGAGTTTGA CCCTATTCCT GTATTGATAA CCAAAAACCC ACAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855222.1
CDS	375..4184
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999454.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999454.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGCTGAAAA AAGGAAGGGT GGGCAGACTA TGGATTCTAG CCTTCCTCTT 
CTAAGTGTAT CTGATCCTTT CATTCCTCTT CAAGTACCTG ATGCACCAGA AAAACTAATT 
GAGGGACTCA AATCTCCTGA CACTTCTCTT CTGCTCCCTG ACCTCTTGCC TATGACAGAT 
CCTTTTGGTA GCACTTCTGA TGCTGTAATT GAAAAAGCTG ATGTTGCTGT TGAGAGTCTG 
ATACCAGGAC TGGAGCCCCC GGTGCCTCAG CGCCTTCCAT CTCAGACGGA ATCTGTGACC 
TCGAACCGCA CAGATTCTCT CACCGGGGAA GATTCCCTGA TTGATTGCTC TCTGCTTTCT 
AACCCCACTA CTGACCTTCT GGAAGAGTTT GCCCCCATAG CCATCTCTGC TCCGGCCCAT 
AAAGCTGCGG AAGATAGTAA TCTCATCTCA GGTTTTGATG TCCCTGAGGG CTCGGACAAG 
GTGGCAGAAG ATGAGTTTGA CCCTATTCCT GTATTGATAA CCAAAAACCC ACAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999455.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855223.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa52199
Clone ID Related Accession (Same CDS sequence)	XM_024999455.1
Accession Version	XM_024999455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855223.1
CDS	374..4177
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999455.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999455.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999456.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855224.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa52200
Clone ID Related Accession (Same CDS sequence)	XM_024999456.1
Accession Version	XM_024999456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT 
GCGGAAGATA GTAATCTCAT CTCAGGTTTT GATGTCCCTG AGGGCTCGGA CAAGGTGGCA 
GAAGATGAGT TTGACCCTAT TCCTGTATTG ATAACCAAAA ACCCACAAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855224.1
CDS	374..4177
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999456.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999456.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGCT 
GCGGAAGATA GTAATCTCAT CTCAGGTTTT GATGTCCCTG AGGGCTCGGA CAAGGTGGCA 
GAAGATGAGT TTGACCCTAT TCCTGTATTG ATAACCAAAA ACCCACAAGG TCTCCAGAGA 
GAGCCCATTT CCTCTCCTGT AATAACTGTA GAGCACTTTA AAGAGTCGAC GGGTGTTAAA 
GGCCTTCCCC TATACCCGGA CCCCTCCCGG GTACCTGGCA CGAAAACTCA GAACAGTTTG 
GAATCTGACT ACTTGGCCCG AGATGGCCCT TCCAGCAACA GCTCGTTCCA CAGCAGCGAA 
GAGGAGGGGA CCGACCTCGA GGGGGACATG CTGGACTGCA GTGGCTCTCG CCCTCTGCTT 
TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT 
ACTCCCTTCA CTCAGCCCGA GGTCACTCCT GCGCAGGCCA AGGCCTCGCA AGGTGGGAGG 
AAGAACCAAT TCGGAGCCCT CATTCAGCCA ATTGCAGATG GACTCGGTGA GCCAGATGTG 
TTCGCCACTG CCCCCTTCCG GAGCTCAAGG ATTCCCGCTG ATGACATTTT TGCCAAAGCC 
CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT TCCCAGCCAG AGGAGACGGA TGTGTTCTTG 
AGAGCTCCCT TTACCAAGAA GAAAGGCATG GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC 
CAGGAGCAGT CCGGTCTCCT CAGTCAGACC GATGACATCC CTCTGCTCCC GGGCCTCGAT 
CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG TATTCCATGG CTGGCTTTGC CCAGCCGTCT 
AACCTCCCCT CCCATCCTGT ACAAGCAGCA GACCATCTTG ACAGCATCTC TTCCAGGGGC 
TCCTCCCTGG AATCTGGGGC CCATCCTAAT GACAGAAACA AAGGACCTCA GCCCCAGAAA 
GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA CCATTCCGCC CCCAGTCTTT ATCCAAATAT 
TCCCGTCACT ATAGCCCGGA AGATGAGCCG AATCCAGAAG CCCAGCCCAT CGCTGCCTAC 
AAAATTGTTT CGCAAACCAA TAAGCAGTCG ATAGCTGGCT CCGTTTCCAT CACATCCCTT 
GCCTCCAGGA CTACGGAGCT GCCAGCTGCT GATCCTTTTG CTTTAGCACC CTTTCCTTCG 
AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010816679.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814981.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

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CloneID	OBa52201
Clone ID Related Accession (Same CDS sequence)	XM_010816679.2 , XM_010810148.2
Accession Version	XM_010816679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814981.1
CDS	397..4188
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010816679.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816679.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_010810148.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808450.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

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CloneID	OBa52201
Clone ID Related Accession (Same CDS sequence)	XM_010816679.2 , XM_010810148.2
Accession Version	XM_010810148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808450.1
CDS	397..4188
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_010810148.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810148.2

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTT 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GAGTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTGCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCCGAGG TCACTCCTGC GCAGGCCAAG GCCTCGCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACGGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGG GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999457.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855225.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

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CloneID	OBa52202
Clone ID Related Accession (Same CDS sequence)	XM_024999457.1
Accession Version	XM_024999457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855225.1
CDS	374..4090
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999457.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999457.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGAGTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTG CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC CGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC GCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC GGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGGGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999459.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855227.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

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CloneID	OBa52203
Clone ID Related Accession (Same CDS sequence)	XM_024999459.1
Accession Version	XM_024999459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855227.1
CDS	374..4066
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999459.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999459.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999464.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855232.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa52205
Clone ID Related Accession (Same CDS sequence)	XM_024999464.1
Accession Version	XM_024999464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGGTCTCC AGAGAGAGCC CATTTCCTCT 
CCTGTAATAA CTGTAGAGCA CTTTAAAGAG TCGACGGGTG TTAAAGGCCT TCCCCTATAC 
CCGGACCCCT CCCGGGTACC TGGCACGAAA ACTCAGAACA GTTTGGAATC TGACTACTTG 
GCCCGAGATG GCCCTTCCAG CAACAGCTCG TTCCACAGCA GCGAAGAGGA GGGGACCGAC 
CTCGAGGGGG ACATGCTGGA CTGCAGTGGC TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA 
GAGGATGAGA GCTGCAAGCC TCCGCAGGGG AAGCTAGGAG GAGCTACTCC CTTCACTCAG 
CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC TCGCAAGGTG GGAGGAAGAA CCAATTCGGA 
GCCCTCATTC AGCCAATTGC AGATGGACTC GGTGAGCCAG ATGTGTTCGC CACTGCCCCC 
TTCCGGAGCT CAAGGATTCC CGCTGATGAC ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG 
GGCAACGTGG CCCCTTCCCA GCCAGAGGAG ACGGATGTGT TCTTGAGAGC TCCCTTTACC 
AAGAAGAAAG GCATGGAGGA GTCAACAGTT GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT 
CTCCTCAGTC AGACCGATGA CATCCCTCTG CTCCCGGGCC TCGATCGAGC CGTGTACCCG 
TCTGCCCGAG CTCAGTATTC CATGGCTGGC TTTGCCCAGC CGTCTAACCT CCCCTCCCAT 
CCTGTACAAG CAGCAGACCA TCTTGACAGC ATCTCTTCCA GGGGCTCCTC CCTGGAATCT 
GGGGCCCATC CTAATGACAG AAACAAAGGA CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC 
CCCGTGGCAG GCAAACCATT CCGCCCCCAG TCTTTATCCA AATATTCCCG TCACTATAGC 
CCGGAAGATG AGCCGAATCC AGAAGCCCAG CCCATCGCTG CCTACAAAAT TGTTTCGCAA 
ACCAATAAGC AGTCGATAGC TGGCTCCGTT TCCATCACAT CCCTTGCCTC CAGGACTACG 
GAGCTGCCAG CTGCTGATCC TTTTGCTTTA GCACCCTTTC CTTCGAAATC AGGCAAACAG 
AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855232.1
CDS	374..3862
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999464.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999464.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGGTCTCC AGAGAGAGCC CATTTCCTCT 
CCTGTAATAA CTGTAGAGCA CTTTAAAGAG TCGACGGGTG TTAAAGGCCT TCCCCTATAC 
CCGGACCCCT CCCGGGTACC TGGCACGAAA ACTCAGAACA GTTTGGAATC TGACTACTTG 
GCCCGAGATG GCCCTTCCAG CAACAGCTCG TTCCACAGCA GCGAAGAGGA GGGGACCGAC 
CTCGAGGGGG ACATGCTGGA CTGCAGTGGC TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA 
GAGGATGAGA GCTGCAAGCC TCCGCAGGGG AAGCTAGGAG GAGCTACTCC CTTCACTCAG 
CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC TCGCAAGGTG GGAGGAAGAA CCAATTCGGA 
GCCCTCATTC AGCCAATTGC AGATGGACTC GGTGAGCCAG ATGTGTTCGC CACTGCCCCC 
TTCCGGAGCT CAAGGATTCC CGCTGATGAC ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG 
GGCAACGTGG CCCCTTCCCA GCCAGAGGAG ACGGATGTGT TCTTGAGAGC TCCCTTTACC 
AAGAAGAAAG GCATGGAGGA GTCAACAGTT GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT 
CTCCTCAGTC AGACCGATGA CATCCCTCTG CTCCCGGGCC TCGATCGAGC CGTGTACCCG 
TCTGCCCGAG CTCAGTATTC CATGGCTGGC TTTGCCCAGC CGTCTAACCT CCCCTCCCAT 
CCTGTACAAG CAGCAGACCA TCTTGACAGC ATCTCTTCCA GGGGCTCCTC CCTGGAATCT 
GGGGCCCATC CTAATGACAG AAACAAAGGA CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC 
CCCGTGGCAG GCAAACCATT CCGCCCCCAG TCTTTATCCA AATATTCCCG TCACTATAGC 
CCGGAAGATG AGCCGAATCC AGAAGCCCAG CCCATCGCTG CCTACAAAAT TGTTTCGCAA 
ACCAATAAGC AGTCGATAGC TGGCTCCGTT TCCATCACAT CCCTTGCCTC CAGGACTACG 
GAGCTGCCAG CTGCTGATCC TTTTGCTTTA GCACCCTTTC CTTCGAAATC AGGCAAACAG 
AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999465.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855233.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X19, mRNA.

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CloneID	OBa52206
Clone ID Related Accession (Same CDS sequence)	XM_024999465.1
Accession Version	XM_024999465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855233.1
CDS	374..3766
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999465.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999465.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGAGTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTGCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCCGAG GTCACTCCTG CGCAGGCCAA GGCCTCGCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACGGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
GGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999467.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855235.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa52207
Clone ID Related Accession (Same CDS sequence)	XM_024999467.1
Accession Version	XM_024999467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC 
TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT 
GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC 
AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC 
TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT 
CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG 
GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA 
GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC 
GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG 
CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG 
TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC 
ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC 
ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT 
CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA 
AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC 
CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA 
GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT 
GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT 
TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855235.1
CDS	374..3661
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_024999467.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999467.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGTCTCCA GAGAGAGCCC ATTTCCTCTC CTGTAATAAC TGTAGAGCAC 
TTTAAAGAGT CGACGGGTGT TAAAGGCCTT CCCCTATACC CGGACCCCTC CCGGGTACCT 
GGCACGAAAA CTCAGAACAG TTTGGAATCT GACTACTTGG CCCGAGATGG CCCTTCCAGC 
AACAGCTCGT TCCACAGCAG CGAAGAGGAG GGGACCGACC TCGAGGGGGA CATGCTGGAC 
TGCAGTGGCT CTCGCCCTCT GCTTTTGGAG TCAGAAGAAG AGGATGAGAG CTGCAAGCCT 
CCGCAGGGGA AGCTAGGAGG AGCTACTCCC TTCACTCAGC CCGAGGTCAC TCCTGCGCAG 
GCCAAGGCCT CGCAAGGTGG GAGGAAGAAC CAATTCGGAG CCCTCATTCA GCCAATTGCA 
GATGGACTCG GTGAGCCAGA TGTGTTCGCC ACTGCCCCCT TCCGGAGCTC AAGGATTCCC 
GCTGATGACA TTTTTGCCAA AGCCCCCTTT GTCTCCAAGG GCAACGTGGC CCCTTCCCAG 
CCAGAGGAGA CGGATGTGTT CTTGAGAGCT CCCTTTACCA AGAAGAAAGG CATGGAGGAG 
TCAACAGTTG CCCAGAGCCC CGCCCAGGAG CAGTCCGGTC TCCTCAGTCA GACCGATGAC 
ATCCCTCTGC TCCCGGGCCT CGATCGAGCC GTGTACCCGT CTGCCCGAGC TCAGTATTCC 
ATGGCTGGCT TTGCCCAGCC GTCTAACCTC CCCTCCCATC CTGTACAAGC AGCAGACCAT 
CTTGACAGCA TCTCTTCCAG GGGCTCCTCC CTGGAATCTG GGGCCCATCC TAATGACAGA 
AACAAAGGAC CTCAGCCCCA GAAAGAAGCT GTCTCAGGCC CCGTGGCAGG CAAACCATTC 
CGCCCCCAGT CTTTATCCAA ATATTCCCGT CACTATAGCC CGGAAGATGA GCCGAATCCA 
GAAGCCCAGC CCATCGCTGC CTACAAAATT GTTTCGCAAA CCAATAAGCA GTCGATAGCT 
GGCTCCGTTT CCATCACATC CCTTGCCTCC AGGACTACGG AGCTGCCAGC TGCTGATCCT 
TTTGCTTTAG CACCCTTTCC TTCGAAATCA GGCAAACAGA AACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_024999468.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855236.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa52208
Clone ID Related Accession (Same CDS sequence)	XM_024999468.1
Accession Version	XM_024999468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTGGGCACTC TAGAAACAGC 
AGTGGGAGCT CTGAGTCCAG TCTTCCCAAC CTAGCCAGGT CTTTGCTGCT GGTGGATCAG 
CTCATAGACC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855236.1
CDS	379..3273
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKGGNLPRSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_024999468.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999468.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGGG TGGGAACCTA CCTAGGTCTG CAACCACCAC TCCTTCAGGC 
TCCCCTCGGG CCTCCCAGCA AAATGTTTAT AATCCTTCAG AAGGTTCTAC CTGGAATCCC 
TTTGATGACG ACAATTTCTC CAAACTGACA GCTGAAGAAC TGCTAAACAA GGACTTTGCC 
AAGCTGGGGG AAGGCAAGTA TCCTGAGAAG CTTGGAGGCT CTGCTGAGAG TTTGATCCCA 
GGCTTTCAAC CAACCCAAGG TGATGCTTTT GCTGCTTCCT CATTTTCTGC TGGAACTGCT 
GAAAAAAGGA AGGGTGGGCA GACTATGGAT TCTAGCCTTC CTCTTCTAAG TGTATCTGAT 
CCTTTCATTC CTCTTCAAGT ACCTGATGCA CCAGAAAAAC TAATTGAGGG ACTCAAATCT 
CCTGACACTT CTCTTCTGCT CCCTGACCTC TTGCCTATGA CAGATCCTTT TGGTAGCACT 
TCTGATGCTG TAATTGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTGGGCACTC TAGAAACAGC 
AGTGGGAGCT CTGAGTCCAG TCTTCCCAAC CTAGCCAGGT CTTTGCTGCT GGTGGATCAG 
CTCATAGACC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473515.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329001.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa91909
Clone ID Related Accession (Same CDS sequence)	XM_015473515.1 , XM_015465398.1
Accession Version	XM_015473515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329001.1
CDS	397..4191
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473515.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473515.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465398.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320884.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa91909
Clone ID Related Accession (Same CDS sequence)	XM_015473515.1 , XM_015465398.1
Accession Version	XM_015465398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320884.1
CDS	397..4191
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465398.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465398.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA AGCTGATGTT GCTGTTGAGA GTCTGATACC AGGACTGGAG 
CCCCCGGTGC CTCAGCGCCT TCCATCTCAG ACGGAATCTG TGACCTCGAA CCGCACAGAT 
TCTCTCACCG GGGAAGATTC CCTGATTGAT TGCTCTCTGC TTTCTAACCC CACTACTGAC 
CTTCTGGAAG AGTTTGCCCC CATAGCCATC TCTGCTCCGG CCCATAAAGC TGCGGAAGAT 
AGTAATCTCA TCTCAGGTTT TGATGTCCCT GAGGGCTCGG ACAAGGTGGC AGAAGATGAG 
TTTGACCCTA TTCCTGTATT GATAACCAAA AACCCACAAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473516.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329002.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

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CloneID	OBa91910
Clone ID Related Accession (Same CDS sequence)	XM_015473516.1 , XM_015465399.1
Accession Version	XM_015473516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329002.1
CDS	397..4182
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473516.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473516.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465399.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320885.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa91910
Clone ID Related Accession (Same CDS sequence)	XM_015473516.1 , XM_015465399.1
Accession Version	XM_015465399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320885.1
CDS	397..4182
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465399.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465399.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTTCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465400.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320886.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa91911
Clone ID Related Accession (Same CDS sequence)	XM_015465400.1 , XM_015473517.1
Accession Version	XM_015465400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320886.1
CDS	397..4182
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465400.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465400.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473517.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329003.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

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CloneID	OBa91911
Clone ID Related Accession (Same CDS sequence)	XM_015465400.1 , XM_015473517.1
Accession Version	XM_015473517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329003.1
CDS	397..4182
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473517.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473517.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGAGTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTGC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCC 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCG CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACG GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGGGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473518.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329004.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa91912
Clone ID Related Accession (Same CDS sequence)	XM_015473518.1 , XM_015465401.1
Accession Version	XM_015473518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329004.1
CDS	397..4095
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473518.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473518.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465401.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320887.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa91912
Clone ID Related Accession (Same CDS sequence)	XM_015473518.1 , XM_015465401.1
Accession Version	XM_015465401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320887.1
CDS	397..4095
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465401.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465401.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG AAAAAGCTGA TGTTGCTGTT 
GAGAGTCTGA TACCAGGACT GGAGCCCCCG GTGCCTCAGC GCCTTCCATC TCAGACGGAA 
TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATTGCTCT 
CTGCTTTCTA ACCCCACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC CATCTCTGCT 
CCGGCCCATA AAGCTGCGGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC 
TCGGACAAGG TGGCAGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA 
CAAGGTCTCC AGAGAGAGCC CATTTCCTCT CCTGTAATAA CTGTAGAGCA CTTTAAAGAG 
TCGACGGGTG TTAAAGGCCT TCCCCTATAC CCGGACCCCT CCCGGGTACC TGGCACGAAA 
ACTCAGAACA GTTTGGAATC TGACTACTTG GCCCGAGATG GCCCTTCCAG CAACAGCTCG 
TTCCACAGCA GCGAAGAGGA GGGGACCGAC CTCGAGGGGG ACATGCTGGA CTGCAGTGGC 
TCTCGCCCTC TGCTTTTGGA GTCAGAAGAA GAGGATGAGA GCTGCAAGCC TCCGCAGGGG 
AAGCTAGGAG GAGCTACTCC CTTCACTCAG CCCGAGGTCA CTCCTGCGCA GGCCAAGGCC 
TCGCAAGGTG GGAGGAAGAA CCAATTCGGA GCCCTCATTC AGCCAATTGC AGATGGACTC 
GGTGAGCCAG ATGTGTTCGC CACTGCCCCC TTCCGGAGCT CAAGGATTCC CGCTGATGAC 
ATTTTTGCCA AAGCCCCCTT TGTCTCCAAG GGCAACGTGG CCCCTTCCCA GCCAGAGGAG 
ACGGATGTGT TCTTGAGAGC TCCCTTTACC AAGAAGAAAG GCATGGAGGA GTCAACAGTT 
GCCCAGAGCC CCGCCCAGGA GCAGTCCGGT CTCCTCAGTC AGACCGATGA CATCCCTCTG 
CTCCCGGGCC TCGATCGAGC CGTGTACCCG TCTGCCCGAG CTCAGTATTC CATGGCTGGC 
TTTGCCCAGC CGTCTAACCT CCCCTCCCAT CCTGTACAAG CAGCAGACCA TCTTGACAGC 
ATCTCTTCCA GGGGCTCCTC CCTGGAATCT GGGGCCCATC CTAATGACAG AAACAAAGGA 
CCTCAGCCCC AGAAAGAAGC TGTCTCAGGC CCCGTGGCAG GCAAACCATT CCGCCCCCAG 
TCTTTATCCA AATATTCCCG TCACTATAGC CCGGAAGATG AGCCGAATCC AGAAGCCCAG 
CCCATCGCTG CCTACAAAAT TGTTTCGCAA ACCAATAAGC AGTCGATAGC TGGCTCCGTT 
TCCATCACAT CCCTTGCCTC CAGGACTACG GAGCTGCCAG CTGCTGATCC TTTTGCTTTA 
GCACCCTTTC CTTCGAAATC AGGCAAACAG AAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473519.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329005.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

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CloneID	OBa91913
Clone ID Related Accession (Same CDS sequence)	XM_015473519.1 , XM_015465402.1
Accession Version	XM_015473519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329005.1
CDS	397..4071
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473519.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473519.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465402.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320888.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa91913
Clone ID Related Accession (Same CDS sequence)	XM_015473519.1 , XM_015465402.1
Accession Version	XM_015465402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320888.1
CDS	397..4071
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465402.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465402.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465405.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320891.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa91916
Clone ID Related Accession (Same CDS sequence)	XM_015465405.1 , XM_015473522.1
Accession Version	XM_015465405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320891.1
CDS	397..3867
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465405.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465405.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473522.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329008.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa91916
Clone ID Related Accession (Same CDS sequence)	XM_015465405.1 , XM_015473522.1
Accession Version	XM_015473522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329008.1
CDS	397..3867
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473522.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473522.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG AGTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTGCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCCGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCGCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACGGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGGG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473523.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329009.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa91917
Clone ID Related Accession (Same CDS sequence)	XM_015473523.1 , XM_015465406.1
Accession Version	XM_015473523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329009.1
CDS	396..3770
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473523.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473523.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465406.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320892.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

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CloneID	OBa91917
Clone ID Related Accession (Same CDS sequence)	XM_015473523.1 , XM_015465406.1
Accession Version	XM_015465406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320892.1
CDS	396..3770
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465406.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465406.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGAGTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTGCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCCG AGGTCACTCC TGCGCAGGCC AAGGCCTCGC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACGG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGGGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465407.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320893.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa91918
Clone ID Related Accession (Same CDS sequence)	XM_015465407.1 , XM_015473524.1
Accession Version	XM_015465407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320893.1
CDS	396..3665
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015465407.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465407.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473524.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329010.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa91918
Clone ID Related Accession (Same CDS sequence)	XM_015465407.1 , XM_015473524.1
Accession Version	XM_015473524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329010.1
CDS	396..3665
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGLQREPISSPVITVEHFKESTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPLLLESEEEDESCKPPQGKLGGATPFTQPEVTPAQAKASQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEETDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPGLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_015473524.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473524.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA GTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTGCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCCGAGGTC ACTCCTGCGC AGGCCAAGGC CTCGCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACGGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGGGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015473525.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329011.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa91919
Clone ID Related Accession (Same CDS sequence)	XM_015473525.1 , XM_015465408.1
Accession Version	XM_015473525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329011.1
CDS	396..2966
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAKT

Target ORF information:

RefSeq Version	XM_015473525.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473525.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_015465408.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320894.1 Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa91919
Clone ID Related Accession (Same CDS sequence)	XM_015473525.1 , XM_015465408.1
Accession Version	XM_015465408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AP2-associated protein kinase 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320894.1
CDS	396..2966
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGTFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAKT

Target ORF information:

RefSeq Version	XM_015465408.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AP2 associated kinase 1 (AAK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465408.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTTG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCAATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACGTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AAK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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