PPEF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPEF2
Entrez Gene ID	530344
Full Name	protein phosphatase with EF-hand domain 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205977.1	NP_001192906.1	serine/threonine-protein phosphatase with EF-hands 2
XM_024993242.1	XP_024849010.1	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
XM_015471683.1	XP_015327169.1	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
NM_001205977.2	NP_001192906.1	serine/threonine-protein phosphatase with EF-hands 2
XM_015471684.1	XP_015327170.1	serine/threonine-protein phosphatase with EF-hands 2 isoform X2
XM_015471683.2	XP_015327169.2	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
NM_001205977.3	NP_001192906.2	serine/threonine-protein phosphatase with EF-hands 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppef2	NP_001100680.1
Gallus gallus (chicken)	PPEF2	XP_420595.3
Drosophila melanogaster (fruit fly)	rdgC	NP_788544.1
Pan troglodytes (chimpanzee)	PPEF2	XP_526584.2
	PPEF2	XP_001100042.1
Mus musculus (house mouse)	Ppef2	NP_035278.1
Homo sapiens (human)	PPEF2	NP_006230.2
Canis lupus familiaris (dog)	PPEF2	XP_544934.3
Xenopus tropicalis (tropical clawed frog)	ppef2	XP_002938615.1
Bos taurus (cattle)	PPEF2	NP_001192906.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011414	XP_317894.4

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01686	NM_001205977.1 Latest version!	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa20523	NM_001205977.2 Latest version!	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa20523	XM_015471683.1 Latest version!	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa235331	NM_001205977.3 Latest version!	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa235331	XM_015471683.2 Latest version!	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa235331	XM_024993242.1 Latest version!	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa90952	XM_015471684.1 Latest version!	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPEF2 ( NM_001205977.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192906.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.

Price & Availability↑

CloneID	OBa01686
Clone ID Related Accession (Same CDS sequence)	NM_001205977.1
Accession Version	NM_001205977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-23
Organism	Bos taurus(Cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03080849.1, AAFC03057121.1 and AAFC03080847.1. On Apr 20, 2011 this sequence version replaced XM_608813.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC ACCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAGAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192906.1
CDS	1..2262
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHMLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	NM_001205977.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205977.1

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC ACCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAGAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( NM_001205977.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192906.1 Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.

Price & Availability↑

CloneID	OBa20523
Clone ID Related Accession (Same CDS sequence)	XM_015471683.1 , NM_001205977.2
Accession Version	NM_001205977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02018120.1. On Jun 30, 2017 this sequence version replaced NM_001205977.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403646, SAMN02403648 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192906.1
CDS	1..2262
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHMLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	NM_001205977.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205977.2

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( XM_015471683.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327169.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X1, mRNA.

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CloneID	OBa20523
Clone ID Related Accession (Same CDS sequence)	XM_015471683.1 , NM_001205977.2
Accession Version	XM_015471683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327169.1
CDS	328..2589
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHMLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	XM_015471683.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471683.1

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( NM_001205977.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192906.2 Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.

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CloneID	OBa235331
Clone ID Related Accession (Same CDS sequence)	XM_024993242.1 , XM_015471683.2 , NM_001205977.3
Accession Version	NM_001205977.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000006.1. On May 30, 2018 this sequence version replaced NM_001205977.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192906.2
CDS	1..2262
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHTLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	NM_001205977.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205977.3

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( XM_024993242.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849010.1 Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235331
Clone ID Related Accession (Same CDS sequence)	XM_024993242.1 , XM_015471683.2 , NM_001205977.3
Accession Version	XM_024993242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849010.1
CDS	185..2446
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHTLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	XM_024993242.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993242.1

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( XM_015471683.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327169.2 Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235331
Clone ID Related Accession (Same CDS sequence)	XM_024993242.1 , XM_015471683.2 , NM_001205977.3
Accession Version	XM_015471683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327169.2
CDS	463..2724
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHTLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGSISLDEFRHTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEQSCSGGDVSDCPQVTNSSNRDCSSPGAH

Target ORF information:

RefSeq Version	XM_015471683.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase with EF-hand domain 2 (PPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471683.2

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAAGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTGCTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA CGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGG TCCATCTCAC TGGACGAGTT CAGGCATACT 
TGGAAGCTAT TCAGCTCTCA TATGAACATT GACATCACAG ACGACTGCAT CTGTGACCTT 
GCCCGGAGCA TTGACTTCAA CAAGGATGGC CACATTGACA TCAATGAATT CCTGGAGGCC 
TTCCGCCTTG TGGAGCAATC CTGCTCAGGG GGTGATGTCT CAGATTGCCC ACAAGTCACA 
AACAGTAGTA ACAGGGACTG CAGCAGCCCA GGTGCACACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF2 ( XM_015471684.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327170.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa90952
Clone ID Related Accession (Same CDS sequence)	XM_015471684.1
Accession Version	XM_015471684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGT CTATCAGAGA TTGCCACAAA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327170.1
CDS	421..2457
Translation	MGSGTSTQHHFAFQNAEKAFRAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHNFFSYLVDHFTPSSHNERDFLNRIFTEERSPQASETQNCGDYDSIEVPDSYTGPRLSFPLLPDHATALVEAFRRKQRLHARYVLNLLHEARKHLVQLPNINQVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEILMVLFAFMLVYPKEFHLNRGNHEDHIVNLRYGFTKEVMHKYKIHGKKILKLLQDVFCWLPLATLVDEKVLILHGGVSDRTDLELLAKLDRHKIVSTMRYKTRKENKKQAEEKGNANQTGSAGRPSPWFLPQSRSLPSSPLRPSTHKAHRAGRSSSVPCGVPLDPKELSRRVRRSVDLELERCRQQAGFPAIKEKEEPSSSESEVDSDAEEQPQPTPEEWRQVVDILWSDPMAQEGCRANTVRGGGCYFGPNVTERLLQKYHLQFLIRSHECKPEGYEFCHSRKVLTIFSASNYYEVGSNRGAYVKLGPELTPHIMQFQANKATHMLTMRQRISRVEESALRALREKLFAHSSDILVEFKKHDKDETGLIALSDWAAAVESVLHLGLPWRMLRPQLVSSLTDNKLAYKSWLENLAKEKLSQQNIQSSLLETLYRNRSNLETIFRIIDSDHSGLSEIATKE

Target ORF information:

RefSeq Version	XM_015471684.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471684.1

ORF Insert Sequence:

ATGGGAAGCG GCACTTCAAC CCAACACCAC TTTGCTTTCC AGAATGCAGA GAAAGCCTTC 
AGGGCAGCAG CCCTGATCCA GAGGTGGTAC CGACGCTACG TGGCCCGCCT GGAGATGAGG 
CGGCGCTGCA CCTGGAGCAT CTTCCAGTCC ATCGAGTATG CCGGGCAGCA AGACCAGGTC 
AAGCTCCACA ATTTCTTCAG CTACCTTGTG GATCACTTCA CCCCCAGCAG TCACAATGAG 
AGGGACTTCC TGAACCGCAT ATTCACTGAG GAGCGATCCC CCCAGGCCTC CGAAACGCAG 
AACTGCGGTG ACTACGACTC CATCGAGGTA CCGGACAGTT ACACAGGGCC ACGCCTCTCC 
TTCCCACTCC TTCCTGACCA TGCAACTGCC CTGGTGGAAG CTTTCAGACG AAAACAAAGG 
CTCCATGCTC GCTATGTCTT GAATCTTTTG CATGAAGCCA GGAAACACCT GGTACAACTG 
CCAAACATCA ACCAGGTCTC CACTTGTTAC AGCGAGGAAA TCACAGTGTG TGGAGATTTA 
CATGGCCAGT TGGATGACTT AATATTTATA TTTTATAAGA ACGGCCTCCC ATCACCAGAG 
CGGGCCTATG TGTTCAATGG CGACTTTGTG GACCGAGGGA AGGATTCAGT CGAGATCCTA 
ATGGTTCTGT TTGCCTTCAT GTTGGTTTAC CCAAAAGAGT TCCATCTTAA CCGAGGAAAC 
CATGAGGACC ATATTGTGAA CTTACGATAT GGTTTTACCA AGGAAGTGAT GCATAAATAT 
AAGATACACG GCAAGAAAAT ACTAAAACTA CTGCAAGATG TGTTCTGCTG GCTTCCACTG 
GCCACTCTGG TTGATGAGAA AGTCCTGATT CTTCACGGTG GAGTGTCAGA CAGGACTGAC 
TTGGAGCTCT TGGCTAAACT AGACAGGCAC AAGATTGTTT CCACCATGAG GTACAAAACA 
AGAAAGGAGA ATAAGAAACA GGCGGAGGAG AAGGGAAACG CCAACCAGAC GGGTTCTGCC 
GGGAGACCCT CCCCGTGGTT TCTCCCCCAA AGCCGCTCTC TGCCCTCCTC GCCCCTCCGC 
CCCAGCACCC ACAAGGCCCA CAGAGCCGGG CGCTCCTCCA GCGTCCCCTG CGGCGTCCCC 
CTGGACCCCA AGGAGCTCTC CCGCCGGGTG CGGCGCTCTG TTGACCTGGA GCTGGAGAGG 
TGCCGGCAGC AAGCGGGCTT CCCGGCGATC AAGGAAAAGG AGGAGCCCTC GTCCTCGGAG 
TCAGAAGTGG ACTCAGATGC CGAGGAGCAG CCGCAGCCCA CACCGGAGGA GTGGAGGCAG 
GTGGTGGATA TACTGTGGAG TGACCCCATG GCTCAGGAGG GCTGCAGGGC CAACACCGTT 
CGAGGAGGCG GCTGTTATTT TGGGCCTAAT GTGACAGAGC GGTTACTACA GAAGTACCAC 
CTGCAGTTCC TGATCCGCTC ACACGAGTGC AAACCTGAAG GCTATGAATT CTGCCACAGT 
CGCAAGGTGT TAACCATCTT TTCTGCCTCC AACTACTACG AAGTTGGCAG CAACAGAGGA 
GCCTATGTCA AACTGGGGCC AGAACTGACC CCACACATCA TGCAGTTTCA AGCTAACAAG 
GCAACCCACA TGCTTACCAT GAGGCAAAGG ATTAGCAGAG TGGAGGAGTC AGCTCTGAGA 
GCCCTACGGG AGAAGTTATT TGCCCATTCC TCAGATATTC TTGTTGAATT TAAGAAGCAT 
GATAAAGATG AAACTGGTCT AATCGCCCTG AGCGACTGGG CTGCAGCCGT GGAGTCCGTG 
CTGCACCTGG GGCTGCCCTG GCGGATGCTG AGGCCACAGC TGGTGAGCAG CTTGACGGAT 
AACAAGCTGG CGTACAAGTC CTGGCTGGAG AACTTGGCCA AAGAAAAACT GAGCCAACAG 
AACATACAGT CAAGTTTGCT GGAAACACTG TATCGCAACC GATCCAACCT GGAGACCATT 
TTTAGGATCA TAGACAGTGA TCACTCAGGT CTATCAGAGA TTGCCACAAA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PPEF2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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