RFFL cDNA ORF clone, Bos taurus(cattle)

The following RFFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09985 XM_015458551.2
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_015467952.1
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005220052.4
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005192868.3
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_024980647.1
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005192866.3
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_024980646.1
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005192867.3
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005220053.3
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005220054.4
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa51793 XM_010798200.2
Latest version!
Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa51793 XM_010815793.3
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa51793 XM_024980649.1
Latest version!
Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
View Old Accession Versions >>
OBa09985 XM_005220052.3 Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa51793 XM_010815793.2 Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_005220054.3 Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa09985 XM_015458551.1 Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005220052.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220109.1
    CDS160..1233
    Translation

    Target ORF information:

    RefSeq Version XM_005220052.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220052.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005220052.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220052.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220109.1
    CDS263..1336
    Translation

    Target ORF information:

    RefSeq Version XM_005220052.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220052.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005192866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646446.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192923.1
    CDS160..1233
    Translation

    Target ORF information:

    RefSeq Version XM_005192866.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_015467952.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646446.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323438.1
    CDS161..1234
    Translation

    Target ORF information:

    RefSeq Version XM_015467952.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_015458551.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314037.1
    CDS153..1226
    Translation

    Target ORF information:

    RefSeq Version XM_015458551.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005220053.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220110.1
    CDS417..1490
    Translation

    Target ORF information:

    RefSeq Version XM_005220053.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220053.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005192868.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646446.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192925.1
    CDS342..1415
    Translation

    Target ORF information:

    RefSeq Version XM_005192868.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192868.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_024980646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836414.1
    CDS436..1509
    Translation

    Target ORF information:

    RefSeq Version XM_024980646.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005220054.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220054.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220111.1
    CDS276..1349
    Translation

    Target ORF information:

    RefSeq Version XM_005220054.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220054.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_024980647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836415.1
    CDS31..1104
    Translation

    Target ORF information:

    RefSeq Version XM_024980647.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005220054.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220111.1
    CDS342..1415
    Translation

    Target ORF information:

    RefSeq Version XM_005220054.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220054.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_015458551.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314037.1
    CDS161..1234
    Translation

    Target ORF information:

    RefSeq Version XM_015458551.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09985
    Clone ID Related Accession (Same CDS sequence) XM_005220052.3 , XM_005220052.4 , XM_005192866.3 , XM_015467952.1 , XM_015458551.2 , XM_005220053.3 , XM_005192868.3 , XM_024980646.1 , XM_005220054.4 , XM_024980647.1 , XM_005220054.3 , XM_015458551.1 , XM_005192867.3
    Accession Version XM_005192867.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646446.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192924.1
    CDS417..1490
    Translation

    Target ORF information:

    RefSeq Version XM_005192867.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGCAGACCT GCTTGGACTG TAAGAAGAAC TTCTGTACCA GCTGTTCCAG CCAAGTGGGG
    GCCGGGCCCC GCCTCTGCCT TCTCTGCCAG CGCTTCCGTG CCACGGACTT CCGGCGGGAG
    GAGCTCATGA AGATGAAGGT GAAGGACCTG AGGGACTATC TCAGCCTCCA CGACATCTCT
    ACCGACATGT GCCGGGAGAA GGAGGAGCTG GTGTCCCTGG TGCTCGGCCA GCAGCCCGTC
    ATCTCCCAGG AGGGCAGGAC TCAGGCGCCC CACTTGGGCC CCGACTTCCC GGAGCAGCAG
    GCCTTCCTCG CCCGGCCCCA CACCAGCACC GTGCCGCCTA CCTCACCCGG CCTCCAGTCT
    GCACCCCCCG GCCAGGCGCA GGGCCGGCAG CAGGCCAATG GCCATGTGTC TCAGGACCAA
    GAGGAGCCCA TCTACCTGGA GCGCACAGCC AGAGCCCCTG CTGAGGACGA GACCCAGTCC
    GTCGACTCAG AGGACAGCTT TGTCCCAGGC CGGAGGGCCT CTCTGTCTGA CCTGACCGAC
    CTGGAGGACA TCGAAGGTCT CACCGTGCGA CAGCTCAAGG AGATCCTGGC TCGCAACTTC
    GTCAACTACA AGGGCTGCTG TGAGAAGTGG GAACTGATGG AGAGGGTGAC GCGGCTGTAC
    AAGGACCAGA AGGGCCTCCA GCACCTGGTG TGCAGCGCTG AAGACCAAAA CGGGGGAGCA
    GTGCCGTCCA GCCTGGAGGA GAACCTGTGT CGGATCTGCA TGGACTCGCC CATCGACTGT
    GTTCTGCTGG AGTGCGGCCA CATGGTCACC TGTACCAAGT GCGGCAAGCG CATGAACGAG
    TGCCCCATCT GCCGGCAGTA CGTGATCCGG GCCGTGCATG TCTTCCGATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51793
    Clone ID Related Accession (Same CDS sequence) XM_010815793.2 , XM_010815793.3 , XM_010798200.2 , XM_024980649.1
    Accession Version XM_010815793.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104490.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814095.1
    CDS161..1231
    Translation

    Target ORF information:

    RefSeq Version XM_010815793.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815793.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51793
    Clone ID Related Accession (Same CDS sequence) XM_010815793.2 , XM_010815793.3 , XM_010798200.2 , XM_024980649.1
    Accession Version XM_010815793.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815793.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814095.1
    CDS264..1334
    Translation

    Target ORF information:

    RefSeq Version XM_010815793.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815793.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51793
    Clone ID Related Accession (Same CDS sequence) XM_010815793.2 , XM_010815793.3 , XM_010798200.2 , XM_024980649.1
    Accession Version XM_010798200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646446.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796502.1
    CDS161..1231
    Translation

    Target ORF information:

    RefSeq Version XM_010798200.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51793
    Clone ID Related Accession (Same CDS sequence) XM_010815793.2 , XM_010815793.3 , XM_010798200.2 , XM_024980649.1
    Accession Version XM_024980649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase rififylin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836417.1
    CDS275..1345
    Translation

    Target ORF information:

    RefSeq Version XM_024980649.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGCCTCACCG 
    CCCCAGGGAG CCAGGACACA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCTCCC
    ACGGGCTCAG AACCAAGCTG CAAGGCGTGT GGGACTCACT TCGCAAGCAT GGCCAGGAAG
    CAGACCTGCT TGGACTGTAA GAAGAACTTC TGTACCAGCT GTTCCAGCCA AGTGGGGGCC
    GGGCCCCGCC TCTGCCTTCT CTGCCAGCGC TTCCGTGCCA CGGACTTCCG GCGGGAGGAG
    CTCATGAAGA TGAAGGTGAA GGACCTGAGG GACTATCTCA GCCTCCACGA CATCTCTACC
    GACATGTGCC GGGAGAAGGA GGAGCTGGTG TCCCTGGTGC TCGGCCAGCA GCCCGTCATC
    TCCCAGGAGG GCAGGACTCA GGCGCCCCAC TTGGGCCCCG ACTTCCCGGA GCAGCAGGCC
    TTCCTCGCCC GGCCCCACAC CAGCACCGTG CCGCCTACCT CACCCGGCCT CCAGTCTGCA
    CCCCCCGGCC AGGCGCAGGG CCGGCAGCAG GCCAATGGCC ATGTGTCTCA GGACCAAGAG
    GAGCCCATCT ACCTGGAGCG CACAGCCAGA GCCCCTGCTG AGGACGAGAC CCAGTCCGTC
    GACTCAGAGG ACAGCTTTGT CCCAGGCCGG AGGGCCTCTC TGTCTGACCT GACCGACCTG
    GAGGACATCG AAGGTCTCAC CGTGCGACAG CTCAAGGAGA TCCTGGCTCG CAACTTCGTC
    AACTACAAGG GCTGCTGTGA GAAGTGGGAA CTGATGGAGA GGGTGACGCG GCTGTACAAG
    GACCAGAAGG GCCTCCAGCA CCTGGTGTGC AGCGCTGAAG ACCAAAACGG GGGAGCAGTG
    CCGTCCAGCC TGGAGGAGAA CCTGTGTCGG ATCTGCATGG ACTCGCCCAT CGACTGTGTT
    CTGCTGGAGT GCGGCCACAT GGTCACCTGT ACCAAGTGCG GCAAGCGCAT GAACGAGTGC
    CCCATCTGCC GGCAGTACGT GATCCGGGCC GTGCATGTCT TCCGATCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.