TNRC6B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005207402.3	XP_005207459.1	trinucleotide repeat-containing gene 6B protein isoform X1
NM_001192655.1	NP_001179584.1	trinucleotide repeat-containing gene 6B protein
XM_005207402.4	XP_005207459.2	trinucleotide repeat-containing gene 6B protein isoform X1
XM_024991832.1	XP_024847600.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_024991835.1	XP_024847603.1	trinucleotide repeat-containing gene 6B protein isoform X6
NM_001192655.2	NP_001179584.1	trinucleotide repeat-containing gene 6B protein
XM_015471407.1	XP_015326893.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_010805751.2	XP_010804053.2	trinucleotide repeat-containing gene 6B protein isoform X1
XM_010805751.1	XP_010804053.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_010805755.2	XP_010804057.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_024991830.1	XP_024847598.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_010805753.3	XP_010804055.2	trinucleotide repeat-containing gene 6B protein isoform X1
XM_024991831.1	XP_024847599.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_024991833.1	XP_024847601.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_010805755.3	XP_010804057.2	trinucleotide repeat-containing gene 6B protein isoform X4
XM_010805753.2	XP_010804055.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_015471405.1	XP_015326891.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_015471406.1	XP_015326892.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_015471408.1	XP_015326894.1	trinucleotide repeat-containing gene 6B protein isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238957	NM_001192655.2 Latest version!	Bos taurus trinucleotide repeat containing adaptor 6B (TNRC6B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234419	XM_010805751.2 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234419	XM_024991830.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234419	XM_005207402.4 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234419	XM_010805753.3 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234420	XM_024991831.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234421	XM_024991832.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234422	XM_010805755.3 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234423	XM_024991833.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234424	XM_024991835.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa40171	NM_001192655.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19988	XM_010805753.2 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19988	XM_010805751.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19988	XM_005207402.3 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19989	XM_015471407.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51515	XM_015471405.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51516	XM_010805755.2 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90831	XM_015471406.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa90832	XM_015471408.1 Latest version!	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

TNRC6B ( NM_001192655.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179584.1 Bos taurus trinucleotide repeat containing adaptor 6B (TNRC6B), mRNA.

Price & Availability↑

CloneID	OBa238957
Clone ID Related Accession (Same CDS sequence)	NM_001192655.2
Accession Version	NM_001192655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-15
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On May 29, 2018 this sequence version replaced NM_001192655.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC TTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGC GTGCACACCC CCGTGCAGCC ACTGAACTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTCATTT CCCCCCAGGT TTCTGCCTCA 
ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAACGT GGGGCCCCAG 
TTATCTCCTC AACAAATTGC CATGCTGAGC CAGCTTCCAC AGATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGTTACAAAA CCAGAGGAAG 
CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG CAGCAGCTGG CTCGGATGGT GAGCGCCCTC 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAGAGGC AGCCCAGCAT GAAGCATTCG 
CCATCTCACC CCGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCCGGCGGCA TGGACTACGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACCCGAGGAG GGTCACCATA CAACCAGTTT 
GACATCATCC CAGGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATC GGAAGTAAAT CTAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTGCTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTTCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACAGCG 
CTGCCCCGCC CGCCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGCT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTCGCCTC CGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCTAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACCTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACTGATG ACGAAGTCAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCGGCA 
ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG 
CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT GGGTCCACGG GGCTCGGGCA GTGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCCGGCGCTT CATTGTGGGG CCCCCCCAAC 
TACTCTTCTA GCTTGTGGGG AGTCCCGACC GTGGAAGACC CTCATAGGAT GGGCAGCCCT 
GCTCCTTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179584.1
CDS	199..5709
Translation	MREKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	NM_001192655.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing adaptor 6B (TNRC6B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192655.2

ORF Insert Sequence:

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC TTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGC GTGCACACCC CCGTGCAGCC ACTGAACTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTCATTT CCCCCCAGGT TTCTGCCTCA 
ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAACGT GGGGCCCCAG 
TTATCTCCTC AACAAATTGC CATGCTGAGC CAGCTTCCAC AGATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGTTACAAAA CCAGAGGAAG 
CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG CAGCAGCTGG CTCGGATGGT GAGCGCCCTC 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAGAGGC AGCCCAGCAT GAAGCATTCG 
CCATCTCACC CCGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCCGGCGGCA TGGACTACGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACCCGAGGAG GGTCACCATA CAACCAGTTT 
GACATCATCC CAGGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATC GGAAGTAAAT CTAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTGCTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTTCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACAGCG 
CTGCCCCGCC CGCCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGCT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTCGCCTC CGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCTAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACCTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACTGATG ACGAAGTCAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCGGCA 
ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG 
CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT GGGTCCACGG GGCTCGGGCA GTGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCCGGCGCTT CATTGTGGGG CCCCCCCAAC 
TACTCTTCTA GCTTGTGGGG AGTCCCGACC GTGGAAGACC CTCATAGGAT GGGCAGCCCT 
GCTCCTTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_005207402.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207459.2 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

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CloneID	OBa234419
Clone ID Related Accession (Same CDS sequence)	XM_005207402.4 , XM_010805751.2 , XM_024991830.1 , XM_010805753.3
Accession Version	XM_005207402.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207402.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207459.2
CDS	455..5902
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_005207402.4
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207402.4

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805751.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804053.2 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa234419
Clone ID Related Accession (Same CDS sequence)	XM_005207402.4 , XM_010805751.2 , XM_024991830.1 , XM_010805753.3
Accession Version	XM_010805751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804053.2
CDS	249..5696
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805751.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805751.2

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_024991830.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847598.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa234419
Clone ID Related Accession (Same CDS sequence)	XM_005207402.4 , XM_010805751.2 , XM_024991830.1 , XM_010805753.3
Accession Version	XM_024991830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847598.1
CDS	265..5712
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_024991830.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991830.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805753.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804055.2 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

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CloneID	OBa234419
Clone ID Related Accession (Same CDS sequence)	XM_005207402.4 , XM_010805751.2 , XM_024991830.1 , XM_010805753.3
Accession Version	XM_010805753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804055.2
CDS	240..5687
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805753.3
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805753.3

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_024991831.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847599.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OBa234420
Clone ID Related Accession (Same CDS sequence)	XM_024991831.1
Accession Version	XM_024991831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC ATGGCAAGAC TCCACCTCCT GGTGAAGAAA GCAAACAAGA GAAGGAGCAC 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAGCGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCACC GTCCTGCATG CTCCTCGGGG GCGGGGCAGG GCCTCCTCCC 
TCCACGGCGC CCGGAGCAAA CCCCAACAAC GCACAAGTGA CAGGAGCGCT GCTGCAGAGC 
GAGGGTGGGA CCGCGCCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AGCGGCGCCT CCCCCAACCC CATTCACATC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC ATGGAAGAGT GGCCTTGCAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCAAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAGGTT CCGGGAACGA ATGTAGTCTG GGGGTCTGGA AATCTGACCC TAAGGCTAGA 
TCTGTTCAAT CTTCCAACTC TACCACGGAG AGCAACAGTG GACTAGGAAA CTGGAGGAAT 
GTGAGTGGTC AGGAGAGGGT TGGACCTGGC TCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAGGG GGCATTGGAA 
ACGGAGACGA GTAATTCCAG TGCACAGGTT AGCACAGTGG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA GCAGGCTCAA 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTC AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGGATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAGGAGAG AGAGGACTCC TGGAAAGGAG CTCCTGTTCA GAAATCAACT 
GGGTCAAAAA GTGACTCTTG GGACAACAAT AACAGGTCTA CGGGTGGGTC CTGGAACTTT 
GGCCCTCAGG ACTCTAATGA CAACAAATGG GGTGAAGGGA ACAAAATGAC ATCCGGGGTC 
TCTCAGGGAG AATGGAAACA GCCGACTGGG TCTGACGAGT TCAAAATTGG AGAATGGAGT 
GGTCCAAACC AACCAAATTC TAGCACTGGA GCATGGGACA ATCAGAAGGG CCACCCCCTC 
CCTGAAAACC AAGGCAATGC CCAGGCTCCC TGTTGGGGAA GATCTTCCAG CTCCACGGGA 
AGTGAAGTTG GAGGTCAAAG CACTGGAAGC AACCACAAAG CAGGAAGTAG TGACAGTCAC 
AATTCTGGCC GTCGGTCGTA CCGGCCCACA CATCCTGATT GTCAGGCTGT CTTGCAGACT 
CTTTTGAGCC GAACTGATTT GGACCCCAGG GTGCTCTCAA ACACTGGCTG GGGCCAAACT 
CAAATTAAGC AGGACACAGT GTGGGACGTT GAAGAGGTGC CAAGGCCCGA GGGGAAATCT 
GACAAAGGAA CTGAGGGGTG GGAGAGCGCT GCCACACAGA CCAAGAGCTC AGGGGGCTGG 
GGAGACGCAC CCAGCCAAAG CAATCAAATG AAGTCTGGAT GGGGAGAGCT CTCAGCCTCT 
ACAGAGTGGA AAGACCCCAA GAACACAGGA GGCTGGAATG ACTATAAGAA CAACAACTCT 
TCCAACTGGG GAGGAGGACG ACCGGACGAG AAGACATCTT CCTCTTGGAA TGAGAACTCC 
AGCAAGGATC AGGGGTGGGG AGGTGGACGC CAGCCCAACC AAGGATGGAC TTCTGGAAAG 
AATGGTTGGG GAGAGGAGGT AGATCAGGCG AAAACCAGCA ATTGGGAAAA TGCTGCAAGT 
AAACCTGTGT CCGGGTGGGG CGAAGGTGGG CAGAGTGAAA TCGGGACTTG GGGGAGTGGT 
GGGAACGCGA GCCTGGCTTC CAAAGGCGGG TGGGAAGACT GCAAAAGGTC TCCGGCATGG 
ACGGAGACAG GCAGACAGCC CAGTTCCTGG AACAAACAGC ACCAACAGCA GCCGCCGCCA 
CAGCAGCTAC CACCACCACA ACCCGAGGCC TCTGGCTCCT GGGGAGGCCC AGCCCCACCA 
CCTCCAGGCA ACGGACGACC TTCCAATTCC AACTGGAGCA GCGGGCCACA GCCAGTAACC 
CCCAAGGATG AGGAACCCAG CGGTTGGGAA GAGCCATCCC CACAGTCGAT CAGTCGGAAG 
ATGGACATTG ACGATGGCAC TTCCGCGTGG GGAGACCCCA ACAGTTATAA CTACAAGAAT 
GTGAATCTGT GGGATAAGAA TTCCCAAGGG GGCCCAGCAC CTCGAGAAGC AAACCTGCCT 
ACCCCGATGA CCGGCAAATC AGCATCAGTC TGGAGCAAAA GCACACCATC TGCTCCAGAT 
AATGGTACTT CAGCTTGGGG TGAGCCAAAC GAAAGCAGTC CTGGGTGGGG CGAGATGGAT 
GACACGGGAG CGTCGACAAC AGGCTGGGGG ACTGCACCCT CCAATGCTCC AAATGCCATG 
AAACCTAATT CCAAATCTAT GCAAGATGGC TGGGGGGAGA ATGACGGGCC AGTGGCAGGA 
ACTCGCCATC CCAGCTGGGA AGAGGAGGAT GATGGAGGAG TCTGGAACAC TGCCGGCTCT 
CAGGGAAGCG CTTCCTCCCA CAACTCAGCA AGCTGGGGAC AAGGAGGAAA GAAACAAATG 
AAGTGCTCAC TCAAAGGAGG AAACAGTGAT TCATGGATGA ATCCTCTTGC CAAACAGTTT 
TCAAATATGG GATTGCTGAG TCAAACTGAA GATAACCCAA GTAGCAAAAT GGATTTGTCT 
GTAGGGAGCC TTCCAGATAA AAAATTTGAT GTGGACAAGC GAGCAATGAA TCTCGGGGAT 
TTTAATGATA TCATGAGGAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG CGTGCACACC CCCGTGCAGC CACTGAACTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTCATT TCCCCCCAGG TTTCTGCCTC AATGCTTAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAACG TGGGGCCCCA GTTATCTCCT 
CAACAAATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGTTACAAA ACCAGAGGAA GCTTTCTCAA 
GCTGTTCGCC AGCAGCAGGA GCAGCAGCTG GCTCGGATGG TGAGCGCCCT CCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGAGG CAGCCCAGCA TGAAGCATTC GCCATCTCAC 
CCCGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCCGGCGGC 
ATGGACTACG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGTGCC CTCTGTAGCC ACACAGGAAG CCAATATGCA CAAAAATGGC 
GCTATTGTGG CCCCTGGTAA GACCCGAGGA GGGTCACCAT ACAACCAGTT TGACATCATC 
CCAGGTGACA CACTGGGTGG CCATACGGGT CCTGCTGGTG ATAGCTGGTT ACCTGCCAAA 
TCTCCACCAA CAAATAAAAT CGGAAGTAAA TCTAGCAATG CCAGTTGGCC TCCAGAATTC 
CAACCAGGAG TGCCATGGAA AGGTATCCAA AACATTGACC CTGAATCTGA CCCCTATGTC 
ACCCCAGGAA GTGTGCTGGG GGGTACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ATAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAC TCCTTTACCA ACGTTCATAG CACTTCAGCA 
AAGTTTCCTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA CCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGC GCTGCCCCGC 
CCGCCTCCCG GTCTGACCAA CCCCAAACCA TCATCTCCCT GGAGCAGCAC AGCACCCCGC 
TCAGTCAGGG GGTGGGGGAC ACAGGACTCA CGGCTCGCCT CCGCCTCTAC CTGGAGTGAT 
GGTGGTTCAG TTCGTCCTAG TTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATTGAT 
GGGTCAACCT TGAGAACCAT CTGCATGCAG CATGGCCCAC TGCTGACATT CCATCTGAAC 
CTAACCCAGG GCACTGCCCT GATCCGATAC AGCACCAAAC AGGAGGCGGC CAAGGCCCAA 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TGGCTGAGTT TGCCACTGAT 
GACGAAGTCA GCCGCTTTCT GGCACAAGCT CAGCCCCCTA CACCTGCGGC AACCCCGAGC 
GCGCCCGCCG CGGGCTGGCA GTCCCTGGAG ACCGGCCAGA CCCAGTCAGA GCCCGTCGGA 
CCTGCTCTGA ATCTTTTCGG TGGGTCCACG GGGCTCGGGC AGTGGAGCAG CAGTGCTGGT 
GGCAGCAGCG GGGCCGATCT TGCCGGCGCT TCATTGTGGG GCCCCCCCAA CTACTCTTCT 
AGCTTGTGGG GAGTCCCGAC CGTGGAAGAC CCTCATAGGA TGGGCAGCCC TGCTCCTTTA 
CTACCTGGTG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847599.1
CDS	309..5690
Translation	MEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_024991831.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991831.1

ORF Insert Sequence:

ATGGAAGGGC ATGGCAAGAC TCCACCTCCT GGTGAAGAAA GCAAACAAGA GAAGGAGCAC 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAGCGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCACC GTCCTGCATG CTCCTCGGGG GCGGGGCAGG GCCTCCTCCC 
TCCACGGCGC CCGGAGCAAA CCCCAACAAC GCACAAGTGA CAGGAGCGCT GCTGCAGAGC 
GAGGGTGGGA CCGCGCCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AGCGGCGCCT CCCCCAACCC CATTCACATC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC ATGGAAGAGT GGCCTTGCAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCAAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAGGTT CCGGGAACGA ATGTAGTCTG GGGGTCTGGA AATCTGACCC TAAGGCTAGA 
TCTGTTCAAT CTTCCAACTC TACCACGGAG AGCAACAGTG GACTAGGAAA CTGGAGGAAT 
GTGAGTGGTC AGGAGAGGGT TGGACCTGGC TCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAGGG GGCATTGGAA 
ACGGAGACGA GTAATTCCAG TGCACAGGTT AGCACAGTGG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA GCAGGCTCAA 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTC AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGGATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAGGAGAG AGAGGACTCC TGGAAAGGAG CTCCTGTTCA GAAATCAACT 
GGGTCAAAAA GTGACTCTTG GGACAACAAT AACAGGTCTA CGGGTGGGTC CTGGAACTTT 
GGCCCTCAGG ACTCTAATGA CAACAAATGG GGTGAAGGGA ACAAAATGAC ATCCGGGGTC 
TCTCAGGGAG AATGGAAACA GCCGACTGGG TCTGACGAGT TCAAAATTGG AGAATGGAGT 
GGTCCAAACC AACCAAATTC TAGCACTGGA GCATGGGACA ATCAGAAGGG CCACCCCCTC 
CCTGAAAACC AAGGCAATGC CCAGGCTCCC TGTTGGGGAA GATCTTCCAG CTCCACGGGA 
AGTGAAGTTG GAGGTCAAAG CACTGGAAGC AACCACAAAG CAGGAAGTAG TGACAGTCAC 
AATTCTGGCC GTCGGTCGTA CCGGCCCACA CATCCTGATT GTCAGGCTGT CTTGCAGACT 
CTTTTGAGCC GAACTGATTT GGACCCCAGG GTGCTCTCAA ACACTGGCTG GGGCCAAACT 
CAAATTAAGC AGGACACAGT GTGGGACGTT GAAGAGGTGC CAAGGCCCGA GGGGAAATCT 
GACAAAGGAA CTGAGGGGTG GGAGAGCGCT GCCACACAGA CCAAGAGCTC AGGGGGCTGG 
GGAGACGCAC CCAGCCAAAG CAATCAAATG AAGTCTGGAT GGGGAGAGCT CTCAGCCTCT 
ACAGAGTGGA AAGACCCCAA GAACACAGGA GGCTGGAATG ACTATAAGAA CAACAACTCT 
TCCAACTGGG GAGGAGGACG ACCGGACGAG AAGACATCTT CCTCTTGGAA TGAGAACTCC 
AGCAAGGATC AGGGGTGGGG AGGTGGACGC CAGCCCAACC AAGGATGGAC TTCTGGAAAG 
AATGGTTGGG GAGAGGAGGT AGATCAGGCG AAAACCAGCA ATTGGGAAAA TGCTGCAAGT 
AAACCTGTGT CCGGGTGGGG CGAAGGTGGG CAGAGTGAAA TCGGGACTTG GGGGAGTGGT 
GGGAACGCGA GCCTGGCTTC CAAAGGCGGG TGGGAAGACT GCAAAAGGTC TCCGGCATGG 
ACGGAGACAG GCAGACAGCC CAGTTCCTGG AACAAACAGC ACCAACAGCA GCCGCCGCCA 
CAGCAGCTAC CACCACCACA ACCCGAGGCC TCTGGCTCCT GGGGAGGCCC AGCCCCACCA 
CCTCCAGGCA ACGGACGACC TTCCAATTCC AACTGGAGCA GCGGGCCACA GCCAGTAACC 
CCCAAGGATG AGGAACCCAG CGGTTGGGAA GAGCCATCCC CACAGTCGAT CAGTCGGAAG 
ATGGACATTG ACGATGGCAC TTCCGCGTGG GGAGACCCCA ACAGTTATAA CTACAAGAAT 
GTGAATCTGT GGGATAAGAA TTCCCAAGGG GGCCCAGCAC CTCGAGAAGC AAACCTGCCT 
ACCCCGATGA CCGGCAAATC AGCATCAGTC TGGAGCAAAA GCACACCATC TGCTCCAGAT 
AATGGTACTT CAGCTTGGGG TGAGCCAAAC GAAAGCAGTC CTGGGTGGGG CGAGATGGAT 
GACACGGGAG CGTCGACAAC AGGCTGGGGG ACTGCACCCT CCAATGCTCC AAATGCCATG 
AAACCTAATT CCAAATCTAT GCAAGATGGC TGGGGGGAGA ATGACGGGCC AGTGGCAGGA 
ACTCGCCATC CCAGCTGGGA AGAGGAGGAT GATGGAGGAG TCTGGAACAC TGCCGGCTCT 
CAGGGAAGCG CTTCCTCCCA CAACTCAGCA AGCTGGGGAC AAGGAGGAAA GAAACAAATG 
AAGTGCTCAC TCAAAGGAGG AAACAGTGAT TCATGGATGA ATCCTCTTGC CAAACAGTTT 
TCAAATATGG GATTGCTGAG TCAAACTGAA GATAACCCAA GTAGCAAAAT GGATTTGTCT 
GTAGGGAGCC TTCCAGATAA AAAATTTGAT GTGGACAAGC GAGCAATGAA TCTCGGGGAT 
TTTAATGATA TCATGAGGAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG CGTGCACACC CCCGTGCAGC CACTGAACTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTCATT TCCCCCCAGG TTTCTGCCTC AATGCTTAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAACG TGGGGCCCCA GTTATCTCCT 
CAACAAATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGTTACAAA ACCAGAGGAA GCTTTCTCAA 
GCTGTTCGCC AGCAGCAGGA GCAGCAGCTG GCTCGGATGG TGAGCGCCCT CCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGAGG CAGCCCAGCA TGAAGCATTC GCCATCTCAC 
CCCGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCCGGCGGC 
ATGGACTACG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGTGCC CTCTGTAGCC ACACAGGAAG CCAATATGCA CAAAAATGGC 
GCTATTGTGG CCCCTGGTAA GACCCGAGGA GGGTCACCAT ACAACCAGTT TGACATCATC 
CCAGGTGACA CACTGGGTGG CCATACGGGT CCTGCTGGTG ATAGCTGGTT ACCTGCCAAA 
TCTCCACCAA CAAATAAAAT CGGAAGTAAA TCTAGCAATG CCAGTTGGCC TCCAGAATTC 
CAACCAGGAG TGCCATGGAA AGGTATCCAA AACATTGACC CTGAATCTGA CCCCTATGTC 
ACCCCAGGAA GTGTGCTGGG GGGTACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ATAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAC TCCTTTACCA ACGTTCATAG CACTTCAGCA 
AAGTTTCCTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA CCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGC GCTGCCCCGC 
CCGCCTCCCG GTCTGACCAA CCCCAAACCA TCATCTCCCT GGAGCAGCAC AGCACCCCGC 
TCAGTCAGGG GGTGGGGGAC ACAGGACTCA CGGCTCGCCT CCGCCTCTAC CTGGAGTGAT 
GGTGGTTCAG TTCGTCCTAG TTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATTGAT 
GGGTCAACCT TGAGAACCAT CTGCATGCAG CATGGCCCAC TGCTGACATT CCATCTGAAC 
CTAACCCAGG GCACTGCCCT GATCCGATAC AGCACCAAAC AGGAGGCGGC CAAGGCCCAA 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TGGCTGAGTT TGCCACTGAT 
GACGAAGTCA GCCGCTTTCT GGCACAAGCT CAGCCCCCTA CACCTGCGGC AACCCCGAGC 
GCGCCCGCCG CGGGCTGGCA GTCCCTGGAG ACCGGCCAGA CCCAGTCAGA GCCCGTCGGA 
CCTGCTCTGA ATCTTTTCGG TGGGTCCACG GGGCTCGGGC AGTGGAGCAG CAGTGCTGGT 
GGCAGCAGCG GGGCCGATCT TGCCGGCGCT TCATTGTGGG GCCCCCCCAA CTACTCTTCT 
AGCTTGTGGG GAGTCCCGAC CGTGGAAGAC CCTCATAGGA TGGGCAGCCC TGCTCCTTTA 
CTACCTGGTG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_024991832.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847600.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

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CloneID	OBa234421
Clone ID Related Accession (Same CDS sequence)	XM_024991832.1
Accession Version	XM_024991832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGA GTCAAACTGA AGATAACCCA AGTAGCAAAA TGGATTTGTC TGTAGGGAGC 
CTTCCAGATA AAAAATTTGA TGTGGACAAG CGAGCAATGA ATCTCGGGGA TTTTAATGAT 
ATCATGAGGA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGGGTGGC AATCATGGTT TGTTTGGAAA TAGCACAGCA 
CAATCGAGAG GCGTGCACAC CCCCGTGCAG CCACTGAACT CTTCTCCTAA TCTCCGGGCG 
CAAGTGCCTC CCCAGTTCAT TTCCCCCCAG GTTTCTGCCT CAATGCTTAA GCAGTTTCCC 
AACAGTGGCC TGAATCCAGG TCTTTTCAAC GTGGGGCCCC AGTTATCTCC TCAACAAATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAGT TGGCATGTCA GCTTCTCCTG 
CAGCAGCAGC AACAGCAGCA GTTGTTACAA AACCAGAGGA AGCTTTCTCA AGCTGTTCGC 
CAGCAGCAGG AGCAGCAGCT GGCTCGGATG GTGAGCGCCC TCCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGAG GCAGCCCAGC ATGAAGCATT CGCCATCTCA CCCCGTTGGG 
CCCAAGCCAC ATCTGGACAA CATGGTACCC AACGCTCTGA ATGTGGGGCT CCCAGACCTT 
CAAACCAAAG GGCCAATACC TGGATATGGT TCTGGCTTCA GCTCCGGCGG CATGGACTAC 
GGCATGGTTG GTGGGAAGGA GGCTGGAACA GAGTCTCGCT TTAAACAGTG GACCTCCATG 
ATGGAGGTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACCCGAGG AGGGTCACCA TACAACCAGT TTGACATCAT CCCAGGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCTAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTGCTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTTCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAG CGCTGCCCCG CCCGCCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG CTCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTCGCC TCCGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCTA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACC 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACTGA TGACGAAGTC 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCGG CAACCCCGAG CGCGCCCGCC 
GCGGGCTGGC AGTCCCTGGA GACCGGCCAG ACCCAGTCAG AGCCCGTCGG ACCTGCTCTG 
AATCTTTTCG GTGGGTCCAC GGGGCTCGGG CAGTGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCCGGCGC TTCATTGTGG GGCCCCCCCA ACTACTCTTC TAGCTTGTGG 
GGAGTCCCGA CCGTGGAAGA CCCTCATAGG ATGGGCAGCC CTGCTCCTTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847600.1
CDS	455..5827
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_024991832.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991832.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGA GTCAAACTGA AGATAACCCA AGTAGCAAAA TGGATTTGTC TGTAGGGAGC 
CTTCCAGATA AAAAATTTGA TGTGGACAAG CGAGCAATGA ATCTCGGGGA TTTTAATGAT 
ATCATGAGGA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGGGTGGC AATCATGGTT TGTTTGGAAA TAGCACAGCA 
CAATCGAGAG GCGTGCACAC CCCCGTGCAG CCACTGAACT CTTCTCCTAA TCTCCGGGCG 
CAAGTGCCTC CCCAGTTCAT TTCCCCCCAG GTTTCTGCCT CAATGCTTAA GCAGTTTCCC 
AACAGTGGCC TGAATCCAGG TCTTTTCAAC GTGGGGCCCC AGTTATCTCC TCAACAAATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAGT TGGCATGTCA GCTTCTCCTG 
CAGCAGCAGC AACAGCAGCA GTTGTTACAA AACCAGAGGA AGCTTTCTCA AGCTGTTCGC 
CAGCAGCAGG AGCAGCAGCT GGCTCGGATG GTGAGCGCCC TCCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGAG GCAGCCCAGC ATGAAGCATT CGCCATCTCA CCCCGTTGGG 
CCCAAGCCAC ATCTGGACAA CATGGTACCC AACGCTCTGA ATGTGGGGCT CCCAGACCTT 
CAAACCAAAG GGCCAATACC TGGATATGGT TCTGGCTTCA GCTCCGGCGG CATGGACTAC 
GGCATGGTTG GTGGGAAGGA GGCTGGAACA GAGTCTCGCT TTAAACAGTG GACCTCCATG 
ATGGAGGTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACCCGAGG AGGGTCACCA TACAACCAGT TTGACATCAT CCCAGGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCTAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTGCTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTTCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAG CGCTGCCCCG CCCGCCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG CTCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTCGCC TCCGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCTA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACC 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACTGA TGACGAAGTC 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCGG CAACCCCGAG CGCGCCCGCC 
GCGGGCTGGC AGTCCCTGGA GACCGGCCAG ACCCAGTCAG AGCCCGTCGG ACCTGCTCTG 
AATCTTTTCG GTGGGTCCAC GGGGCTCGGG CAGTGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCCGGCGC TTCATTGTGG GGCCCCCCCA ACTACTCTTC TAGCTTGTGG 
GGAGTCCCGA CCGTGGAAGA CCCTCATAGG ATGGGCAGCC CTGCTCCTTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805755.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804057.2 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OBa234422
Clone ID Related Accession (Same CDS sequence)	XM_010805755.3
Accession Version	XM_010805755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GGGTGGCAAT 
CATGGTTTGT TTGGAAATAG CACAGCACAA TCGAGAGGCG TGCACACCCC CGTGCAGCCA 
CTGAACTCTT CTCCTAATCT CCGGGCGCAA GTGCCTCCCC AGTTCATTTC CCCCCAGGTT 
TCTGCCTCAA TGCTTAAGCA GTTTCCCAAC AGTGGCCTGA ATCCAGGTCT TTTCAACGTG 
GGGCCCCAGT TATCTCCTCA ACAAATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAGTTGG CATGTCAGCT TCTCCTGCAG CAGCAGCAAC AGCAGCAGTT GTTACAAAAC 
CAGAGGAAGC TTTCTCAAGC TGTTCGCCAG CAGCAGGAGC AGCAGCTGGC TCGGATGGTG 
AGCGCCCTCC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGAGGCA GCCCAGCATG 
AAGCATTCGC CATCTCACCC CGTTGGGCCC AAGCCACATC TGGACAACAT GGTACCCAAC 
GCTCTGAATG TGGGGCTCCC AGACCTTCAA ACCAAAGGGC CAATACCTGG ATATGGTTCT 
GGCTTCAGCT CCGGCGGCAT GGACTACGGC ATGGTTGGTG GGAAGGAGGC TGGAACAGAG 
TCTCGCTTTA AACAGTGGAC CTCCATGATG GAGGTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CCCGAGGAGG GTCACCATAC 
AACCAGTTTG ACATCATCCC AGGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC TAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTGCTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTTCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACAGCGC TGCCCCGCCC GCCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGCTC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTCGCCTCC 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCTAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACCTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACTGATGA CGAAGTCAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCGGCAA CCCCGAGCGC GCCCGCCGCG GGCTGGCAGT CCCTGGAGAC CGGCCAGACC 
CAGTCAGAGC CCGTCGGACC TGCTCTGAAT CTTTTCGGTG GGTCCACGGG GCTCGGGCAG 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CCGGCGCTTC ATTGTGGGGC 
CCCCCCAACT ACTCTTCTAG CTTGTGGGGA GTCCCGACCG TGGAAGACCC TCATAGGATG 
GGCAGCCCTG CTCCTTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804057.2
CDS	798..6137
Translation	MREKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805755.3
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805755.3

ORF Insert Sequence:

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GGGTGGCAAT 
CATGGTTTGT TTGGAAATAG CACAGCACAA TCGAGAGGCG TGCACACCCC CGTGCAGCCA 
CTGAACTCTT CTCCTAATCT CCGGGCGCAA GTGCCTCCCC AGTTCATTTC CCCCCAGGTT 
TCTGCCTCAA TGCTTAAGCA GTTTCCCAAC AGTGGCCTGA ATCCAGGTCT TTTCAACGTG 
GGGCCCCAGT TATCTCCTCA ACAAATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAGTTGG CATGTCAGCT TCTCCTGCAG CAGCAGCAAC AGCAGCAGTT GTTACAAAAC 
CAGAGGAAGC TTTCTCAAGC TGTTCGCCAG CAGCAGGAGC AGCAGCTGGC TCGGATGGTG 
AGCGCCCTCC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGAGGCA GCCCAGCATG 
AAGCATTCGC CATCTCACCC CGTTGGGCCC AAGCCACATC TGGACAACAT GGTACCCAAC 
GCTCTGAATG TGGGGCTCCC AGACCTTCAA ACCAAAGGGC CAATACCTGG ATATGGTTCT 
GGCTTCAGCT CCGGCGGCAT GGACTACGGC ATGGTTGGTG GGAAGGAGGC TGGAACAGAG 
TCTCGCTTTA AACAGTGGAC CTCCATGATG GAGGTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CCCGAGGAGG GTCACCATAC 
AACCAGTTTG ACATCATCCC AGGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC TAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTGCTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTTCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACAGCGC TGCCCCGCCC GCCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGCTC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTCGCCTCC 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCTAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACCTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACTGATGA CGAAGTCAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCGGCAA CCCCGAGCGC GCCCGCCGCG GGCTGGCAGT CCCTGGAGAC CGGCCAGACC 
CAGTCAGAGC CCGTCGGACC TGCTCTGAAT CTTTTCGGTG GGTCCACGGG GCTCGGGCAG 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CCGGCGCTTC ATTGTGGGGC 
CCCCCCAACT ACTCTTCTAG CTTGTGGGGA GTCCCGACCG TGGAAGACCC TCATAGGATG 
GGCAGCCCTG CTCCTTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_024991833.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847601.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OBa234423
Clone ID Related Accession (Same CDS sequence)	XM_024991833.1
Accession Version	XM_024991833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGATTCCA AATCTATGCA AGATGGCTGG 
GGGGAGAATG ACGGGCCAGT GGCAGGAACT CGCCATCCCA GCTGGGAAGA GGAGGATGAT 
GGAGGAGTCT GGAACACTGC CGGCTCTCAG GGAAGCGCTT CCTCCCACAA CTCAGCAAGC 
TGGGGACAAG GAGGAAAGAA ACAAATGAAG TGCTCACTCA AAGGAGGAAA CAGTGATTCA 
TGGATGAATC CTCTTGCCAA ACAGTTTTCA AATATGGGAT TGCTGAGTCA AACTGAAGAT 
AACCCAAGTA GCAAAATGGA TTTGTCTGTA GGGAGCCTTC CAGATAAAAA ATTTGATGTG 
GACAAGCGAG CAATGAATCT CGGGGATTTT AATGATATCA TGAGGAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
GGTGGCAATC ATGGTTTGTT TGGAAATAGC ACAGCACAAT CGAGAGGCGT GCACACCCCC 
GTGCAGCCAC TGAACTCTTC TCCTAATCTC CGGGCGCAAG TGCCTCCCCA GTTCATTTCC 
CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG TTTCCCAACA GTGGCCTGAA TCCAGGTCTT 
TTCAACGTGG GGCCCCAGTT ATCTCCTCAA CAAATTGCCA TGCTGAGCCA GCTTCCACAG 
ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT CTCCTGCAGC AGCAGCAACA GCAGCAGTTG 
TTACAAAACC AGAGGAAGCT TTCTCAAGCT GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT 
CGGATGGTGA GCGCCCTCCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGAGGCAG 
CCCAGCATGA AGCATTCGCC ATCTCACCCC GTTGGGCCCA AGCCACATCT GGACAACATG 
GTACCCAACG CTCTGAATGT GGGGCTCCCA GACCTTCAAA CCAAAGGGCC AATACCTGGA 
TATGGTTCTG GCTTCAGCTC CGGCGGCATG GACTACGGCA TGGTTGGTGG GAAGGAGGCT 
GGAACAGAGT CTCGCTTTAA ACAGTGGACC TCCATGATGG AGGTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG 
TCACCATACA ACCAGTTTGA CATCATCCCA GGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCT 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TGCTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTTCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACAGCGCT GCCCCGCCCG CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGCTCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTCGCCTCCG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACCTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACTGATGAC GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCGGCAAC CCCGAGCGCG CCCGCCGCGG GCTGGCAGTC CCTGGAGACC 
GGCCAGACCC AGTCAGAGCC CGTCGGACCT GCTCTGAATC TTTTCGGTGG GTCCACGGGG 
CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC CGGCGCTTCA 
TTGTGGGGCC CCCCCAACTA CTCTTCTAGC TTGTGGGGAG TCCCGACCGT GGAAGACCCT 
CATAGGATGG GCAGCCCTGC TCCTTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847601.1
CDS	455..5743
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASDSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_024991833.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991833.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGC GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGATTCCA AATCTATGCA AGATGGCTGG 
GGGGAGAATG ACGGGCCAGT GGCAGGAACT CGCCATCCCA GCTGGGAAGA GGAGGATGAT 
GGAGGAGTCT GGAACACTGC CGGCTCTCAG GGAAGCGCTT CCTCCCACAA CTCAGCAAGC 
TGGGGACAAG GAGGAAAGAA ACAAATGAAG TGCTCACTCA AAGGAGGAAA CAGTGATTCA 
TGGATGAATC CTCTTGCCAA ACAGTTTTCA AATATGGGAT TGCTGAGTCA AACTGAAGAT 
AACCCAAGTA GCAAAATGGA TTTGTCTGTA GGGAGCCTTC CAGATAAAAA ATTTGATGTG 
GACAAGCGAG CAATGAATCT CGGGGATTTT AATGATATCA TGAGGAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
GGTGGCAATC ATGGTTTGTT TGGAAATAGC ACAGCACAAT CGAGAGGCGT GCACACCCCC 
GTGCAGCCAC TGAACTCTTC TCCTAATCTC CGGGCGCAAG TGCCTCCCCA GTTCATTTCC 
CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG TTTCCCAACA GTGGCCTGAA TCCAGGTCTT 
TTCAACGTGG GGCCCCAGTT ATCTCCTCAA CAAATTGCCA TGCTGAGCCA GCTTCCACAG 
ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT CTCCTGCAGC AGCAGCAACA GCAGCAGTTG 
TTACAAAACC AGAGGAAGCT TTCTCAAGCT GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT 
CGGATGGTGA GCGCCCTCCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGAGGCAG 
CCCAGCATGA AGCATTCGCC ATCTCACCCC GTTGGGCCCA AGCCACATCT GGACAACATG 
GTACCCAACG CTCTGAATGT GGGGCTCCCA GACCTTCAAA CCAAAGGGCC AATACCTGGA 
TATGGTTCTG GCTTCAGCTC CGGCGGCATG GACTACGGCA TGGTTGGTGG GAAGGAGGCT 
GGAACAGAGT CTCGCTTTAA ACAGTGGACC TCCATGATGG AGGTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG 
TCACCATACA ACCAGTTTGA CATCATCCCA GGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCT 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TGCTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTTCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACAGCGCT GCCCCGCCCG CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGCTCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTCGCCTCCG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACCTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACTGATGAC GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCGGCAAC CCCGAGCGCG CCCGCCGCGG GCTGGCAGTC CCTGGAGACC 
GGCCAGACCC AGTCAGAGCC CGTCGGACCT GCTCTGAATC TTTTCGGTGG GTCCACGGGG 
CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC CGGCGCTTCA 
TTGTGGGGCC CCCCCAACTA CTCTTCTAGC TTGTGGGGAG TCCCGACCGT GGAAGACCCT 
CATAGGATGG GCAGCCCTGC TCCTTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_024991835.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847603.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

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CloneID	OBa234424
Clone ID Related Accession (Same CDS sequence)	XM_024991835.1
Accession Version	XM_024991835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGATTC CAAATCTATG 
CAAGATGGCT GGGGGGAGAA TGACGGGCCA GTGGCAGGAA CTCGCCATCC CAGCTGGGAA 
GAGGAGGATG ATGGAGGAGT CTGGAACACT GCCGGCTCTC AGGGAAGCGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAACAAATGA AGTGCTCACT CAAAGGAGGA 
AACAGTGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAACCCAAG TAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGATG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTTGAGA AGGGTGGCAA TCATGGTTTG TTTGGAAATA GCACAGCACA ATCGAGAGGC 
GTGCACACCC CCGTGCAGCC ACTGAACTCT TCTCCTAATC TCCGGGCGCA AGTGCCTCCC 
CAGTTCATTT CCCCCCAGGT TTCTGCCTCA ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG 
AATCCAGGTC TTTTCAACGT GGGGCCCCAG TTATCTCCTC AACAAATTGC CATGCTGAGC 
CAGCTTCCAC AGATTCCCCA GTTTCAGTTG GCATGTCAGC TTCTCCTGCA GCAGCAGCAA 
CAGCAGCAGT TGTTACAAAA CCAGAGGAAG CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG 
CAGCAGCTGG CTCGGATGGT GAGCGCCCTC CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGAGGC AGCCCAGCAT GAAGCATTCG CCATCTCACC CCGTTGGGCC CAAGCCACAT 
CTGGACAACA TGGTACCCAA CGCTCTGAAT GTGGGGCTCC CAGACCTTCA AACCAAAGGG 
CCAATACCTG GATATGGTTC TGGCTTCAGC TCCGGCGGCA TGGACTACGG CATGGTTGGT 
GGGAAGGAGG CTGGAACAGA GTCTCGCTTT AAACAGTGGA CCTCCATGAT GGAGGTGCCC 
TCTGTAGCCA CACAGGAAGC CAATATGCAC AAAAATGGCG CTATTGTGGC CCCTGGTAAG 
ACCCGAGGAG GGTCACCATA CAACCAGTTT GACATCATCC CAGGTGACAC ACTGGGTGGC 
CATACGGGTC CTGCTGGTGA TAGCTGGTTA CCTGCCAAAT CTCCACCAAC AAATAAAATC 
GGAAGTAAAT CTAGCAATGC CAGTTGGCCT CCAGAATTCC AACCAGGAGT GCCATGGAAA 
GGTATCCAAA ACATTGACCC TGAATCTGAC CCCTATGTCA CCCCAGGAAG TGTGCTGGGG 
GGTACAGCCA CATCTCCCAT TGTAGATACT GACCACCAAC TTCTGCGGGA TAACACCACA 
GGGTCTAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTACAGTGCC 
TCTGACAACT CCTTTACCAA CGTTCATAGC ACTTCAGCAA AGTTTCCTGA TTACAAATCA 
ACATGGTCCC CAGATCCCAT AGGACACAAC CCCACTCATC TCTCCAACAA GATGTGGAAA 
AACCATATTT CCTCAAGGAA CACTACAGCG CTGCCCCGCC CGCCTCCCGG TCTGACCAAC 
CCCAAACCAT CATCTCCCTG GAGCAGCACA GCACCCCGCT CAGTCAGGGG GTGGGGGACA 
CAGGACTCAC GGCTCGCCTC CGCCTCTACC TGGAGTGATG GTGGTTCAGT TCGTCCTAGT 
TACTGGCTGG TTCTTCACAA TCTCACTCCA CAGATTGATG GGTCAACCTT GAGAACCATC 
TGCATGCAGC ATGGCCCACT GCTGACATTC CATCTGAACC TAACCCAGGG CACTGCCCTG 
ATCCGATACA GCACCAAACA GGAGGCGGCC AAGGCCCAAA CTGCACTGCA CATGTGTGTG 
TTGGGAAACA CTACCATCCT GGCTGAGTTT GCCACTGATG ACGAAGTCAG CCGCTTTCTG 
GCACAAGCTC AGCCCCCTAC ACCTGCGGCA ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG 
TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT 
GGGTCCACGG GGCTCGGGCA GTGGAGCAGC AGTGCTGGTG GCAGCAGCGG GGCCGATCTT 
GCCGGCGCTT CATTGTGGGG CCCCCCCAAC TACTCTTCTA GCTTGTGGGG AGTCCCGACC 
GTGGAAGACC CTCATAGGAT GGGCAGCCCT GCTCCTTTAC TACCTGGTGA CCTTCTGGGA 
GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847603.1
CDS	206..5386
Translation	MREKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASDSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_024991835.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991835.1

ORF Insert Sequence:

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGATTC CAAATCTATG 
CAAGATGGCT GGGGGGAGAA TGACGGGCCA GTGGCAGGAA CTCGCCATCC CAGCTGGGAA 
GAGGAGGATG ATGGAGGAGT CTGGAACACT GCCGGCTCTC AGGGAAGCGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAACAAATGA AGTGCTCACT CAAAGGAGGA 
AACAGTGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAACCCAAG TAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGATG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTTGAGA AGGGTGGCAA TCATGGTTTG TTTGGAAATA GCACAGCACA ATCGAGAGGC 
GTGCACACCC CCGTGCAGCC ACTGAACTCT TCTCCTAATC TCCGGGCGCA AGTGCCTCCC 
CAGTTCATTT CCCCCCAGGT TTCTGCCTCA ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG 
AATCCAGGTC TTTTCAACGT GGGGCCCCAG TTATCTCCTC AACAAATTGC CATGCTGAGC 
CAGCTTCCAC AGATTCCCCA GTTTCAGTTG GCATGTCAGC TTCTCCTGCA GCAGCAGCAA 
CAGCAGCAGT TGTTACAAAA CCAGAGGAAG CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG 
CAGCAGCTGG CTCGGATGGT GAGCGCCCTC CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGAGGC AGCCCAGCAT GAAGCATTCG CCATCTCACC CCGTTGGGCC CAAGCCACAT 
CTGGACAACA TGGTACCCAA CGCTCTGAAT GTGGGGCTCC CAGACCTTCA AACCAAAGGG 
CCAATACCTG GATATGGTTC TGGCTTCAGC TCCGGCGGCA TGGACTACGG CATGGTTGGT 
GGGAAGGAGG CTGGAACAGA GTCTCGCTTT AAACAGTGGA CCTCCATGAT GGAGGTGCCC 
TCTGTAGCCA CACAGGAAGC CAATATGCAC AAAAATGGCG CTATTGTGGC CCCTGGTAAG 
ACCCGAGGAG GGTCACCATA CAACCAGTTT GACATCATCC CAGGTGACAC ACTGGGTGGC 
CATACGGGTC CTGCTGGTGA TAGCTGGTTA CCTGCCAAAT CTCCACCAAC AAATAAAATC 
GGAAGTAAAT CTAGCAATGC CAGTTGGCCT CCAGAATTCC AACCAGGAGT GCCATGGAAA 
GGTATCCAAA ACATTGACCC TGAATCTGAC CCCTATGTCA CCCCAGGAAG TGTGCTGGGG 
GGTACAGCCA CATCTCCCAT TGTAGATACT GACCACCAAC TTCTGCGGGA TAACACCACA 
GGGTCTAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTACAGTGCC 
TCTGACAACT CCTTTACCAA CGTTCATAGC ACTTCAGCAA AGTTTCCTGA TTACAAATCA 
ACATGGTCCC CAGATCCCAT AGGACACAAC CCCACTCATC TCTCCAACAA GATGTGGAAA 
AACCATATTT CCTCAAGGAA CACTACAGCG CTGCCCCGCC CGCCTCCCGG TCTGACCAAC 
CCCAAACCAT CATCTCCCTG GAGCAGCACA GCACCCCGCT CAGTCAGGGG GTGGGGGACA 
CAGGACTCAC GGCTCGCCTC CGCCTCTACC TGGAGTGATG GTGGTTCAGT TCGTCCTAGT 
TACTGGCTGG TTCTTCACAA TCTCACTCCA CAGATTGATG GGTCAACCTT GAGAACCATC 
TGCATGCAGC ATGGCCCACT GCTGACATTC CATCTGAACC TAACCCAGGG CACTGCCCTG 
ATCCGATACA GCACCAAACA GGAGGCGGCC AAGGCCCAAA CTGCACTGCA CATGTGTGTG 
TTGGGAAACA CTACCATCCT GGCTGAGTTT GCCACTGATG ACGAAGTCAG CCGCTTTCTG 
GCACAAGCTC AGCCCCCTAC ACCTGCGGCA ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG 
TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT 
GGGTCCACGG GGCTCGGGCA GTGGAGCAGC AGTGCTGGTG GCAGCAGCGG GGCCGATCTT 
GCCGGCGCTT CATTGTGGGG CCCCCCCAAC TACTCTTCTA GCTTGTGGGG AGTCCCGACC 
GTGGAAGACC CTCATAGGAT GGGCAGCCCT GCTCCTTTAC TACCTGGTGA CCTTCTGGGA 
GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( NM_001192655.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179584.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), mRNA.

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CloneID	OBa40171
Clone ID Related Accession (Same CDS sequence)	NM_001192655.1
Accession Version	NM_001192655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03089873.1. On Jul 17, 2010 this sequence version replaced XM_606579.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC TTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGC GTGCACACCC CCGTGCAGCC ACTGAACTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTCATTT CCCCCCAGGT TTCTGCCTCA 
ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAACGT GGGGCCCCAG 
TTATCTCCTC AACAAATTGC CATGCTGAGC CAGCTTCCAC AGATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGTTACAAAA CCAGAGGAAG 
CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG CAGCAGCTGG CTCGGATGGT GAGCGCCCTC 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAGAGGC AGCCCAGCAT GAAGCATTCG 
CCATCTCACC CCGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCCGGCGGCA TGGACTACGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACCCGAGGAG GGTCACCATA CAACCAGTTT 
GACATCATCC CAGGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATT GGAAGTAAAT CTAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTGCTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTTCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACAGCG 
CTGCCCCGCC CGCCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGCT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTCGCCTC CGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCTAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACCTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACTGATG ACGAAGTCAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCGGCA 
ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG 
CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT GGGTCCACGG GGCTCGGGCA GTGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCCGGCGCTT CATTGTGGGG CCCCCCCAAC 
TACTCTTCTA GCTTGTGGGG AGTCCCGACC GTGGAAGACC CTCATAGGAT GGGCAGCCCT 
GCTCCTTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179584.1
CDS	199..5709
Translation	MREKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	NM_001192655.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192655.1

ORF Insert Sequence:

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GCGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC TTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGC GTGCACACCC CCGTGCAGCC ACTGAACTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTCATTT CCCCCCAGGT TTCTGCCTCA 
ATGCTTAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAACGT GGGGCCCCAG 
TTATCTCCTC AACAAATTGC CATGCTGAGC CAGCTTCCAC AGATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGTTACAAAA CCAGAGGAAG 
CTTTCTCAAG CTGTTCGCCA GCAGCAGGAG CAGCAGCTGG CTCGGATGGT GAGCGCCCTC 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAGAGGC AGCCCAGCAT GAAGCATTCG 
CCATCTCACC CCGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCCGGCGGCA TGGACTACGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACCCGAGGAG GGTCACCATA CAACCAGTTT 
GACATCATCC CAGGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATT GGAAGTAAAT CTAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTGCTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTTCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACAGCG 
CTGCCCCGCC CGCCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGCT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTCGCCTC CGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCTAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACCTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACTGATG ACGAAGTCAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCGGCA 
ACCCCGAGCG CGCCCGCCGC GGGCTGGCAG TCCCTGGAGA CCGGCCAGAC CCAGTCAGAG 
CCCGTCGGAC CTGCTCTGAA TCTTTTCGGT GGGTCCACGG GGCTCGGGCA GTGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCCGGCGCTT CATTGTGGGG CCCCCCCAAC 
TACTCTTCTA GCTTGTGGGG AGTCCCGACC GTGGAAGACC CTCATAGGAT GGGCAGCCCT 
GCTCCTTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_005207402.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207459.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

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CloneID	OBa19988
Clone ID Related Accession (Same CDS sequence)	XM_005207402.3 , XM_010805751.1 , XM_010805753.2
Accession Version	XM_005207402.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207459.1
CDS	345..5792
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_005207402.3
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207402.3

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805751.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804053.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

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CloneID	OBa19988
Clone ID Related Accession (Same CDS sequence)	XM_005207402.3 , XM_010805751.1 , XM_010805753.2
Accession Version	XM_010805751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804053.1
CDS	248..5695
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805751.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805751.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805753.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804055.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

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CloneID	OBa19988
Clone ID Related Accession (Same CDS sequence)	XM_005207402.3 , XM_010805751.1 , XM_010805753.2
Accession Version	XM_010805753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804055.1
CDS	239..5686
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805753.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805753.2

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGCGTG CACACCCCCG TGCAGCCACT GAACTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTCATTTCCC CCCAGGTTTC TGCCTCAATG 
CTTAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAACGTGGG GCCCCAGTTA 
TCTCCTCAAC AAATTGCCAT GCTGAGCCAG CTTCCACAGA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGT TACAAAACCA GAGGAAGCTT 
TCTCAAGCTG TTCGCCAGCA GCAGGAGCAG CAGCTGGCTC GGATGGTGAG CGCCCTCCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGAGGCAGC CCAGCATGAA GCATTCGCCA 
TCTCACCCCG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCC 
GGCGGCATGG ACTACGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACC CGAGGAGGGT CACCATACAA CCAGTTTGAC 
ATCATCCCAG GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCTA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT GCTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TTCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACAGCGCTG 
CCCCGCCCGC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGCTCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TCGCCTCCGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCTAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACCTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACTGATGACG AAGTCAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCGGCAACC 
CCGAGCGCGC CCGCCGCGGG CTGGCAGTCC CTGGAGACCG GCCAGACCCA GTCAGAGCCC 
GTCGGACCTG CTCTGAATCT TTTCGGTGGG TCCACGGGGC TCGGGCAGTG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCC GGCGCTTCAT TGTGGGGCCC CCCCAACTAC 
TCTTCTAGCT TGTGGGGAGT CCCGACCGTG GAAGACCCTC ATAGGATGGG CAGCCCTGCT 
CCTTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015471407.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326893.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OBa19989
Clone ID Related Accession (Same CDS sequence)	XM_015471407.1
Accession Version	XM_015471407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGATTCCA AATCTATGCA AGATGGCTGG 
GGGGAGAATG ACGGGCCAGT GGCAGGAACT CGCCATCCCA GCTGGGAAGA GGAGGATGAT 
GGAGGAGTCT GGAACACTGC CGGCTCTCAG GGAAGCGCTT CCTCCCACAA CTCAGCAAGC 
TGGGGACAAG GAGGAAAGAA ACAAATGAAG TGCTCACTCA AAGGAGGAAA CAGTGATTCA 
TGGATGAATC CTCTTGCCAA ACAGTTTTCA AATATGGGAT TGCTGAGTCA AACTGAAGAT 
AACCCAAGTA GCAAAATGGA TTTGTCTGTA GGGAGCCTTC CAGATAAAAA ATTTGATGTG 
GACAAGCGAG CAATGAATCT CGGGGATTTT AATGATATCA TGAGGAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
GGTGGCAATC ATGGTTTGTT TGGAAATAGC ACAGCACAAT CGAGAGGCGT GCACACCCCC 
GTGCAGCCAC TGAACTCTTC TCCTAATCTC CGGGCGCAAG TGCCTCCCCA GTTCATTTCC 
CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG TTTCCCAACA GTGGCCTGAA TCCAGGTCTT 
TTCAACGTGG GGCCCCAGTT ATCTCCTCAA CAAATTGCCA TGCTGAGCCA GCTTCCACAG 
ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT CTCCTGCAGC AGCAGCAACA GCAGCAGTTG 
TTACAAAACC AGAGGAAGCT TTCTCAAGCT GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT 
CGGATGGTGA GCGCCCTCCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGAGGCAG 
CCCAGCATGA AGCATTCGCC ATCTCACCCC GTTGGGCCCA AGCCACATCT GGACAACATG 
GTACCCAACG CTCTGAATGT GGGGCTCCCA GACCTTCAAA CCAAAGGGCC AATACCTGGA 
TATGGTTCTG GCTTCAGCTC CGGCGGCATG GACTACGGCA TGGTTGGTGG GAAGGAGGCT 
GGAACAGAGT CTCGCTTTAA ACAGTGGACC TCCATGATGG AGGTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG 
TCACCATACA ACCAGTTTGA CATCATCCCA GGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCT 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TGCTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTTCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACAGCGCT GCCCCGCCCG CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGCTCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTCGCCTCCG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACCTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACTGATGAC GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCGGCAAC CCCGAGCGCG CCCGCCGCGG GCTGGCAGTC CCTGGAGACC 
GGCCAGACCC AGTCAGAGCC CGTCGGACCT GCTCTGAATC TTTTCGGTGG GTCCACGGGG 
CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC CGGCGCTTCA 
TTGTGGGGCC CCCCCAACTA CTCTTCTAGC TTGTGGGGAG TCCCGACCGT GGAAGACCCT 
CATAGGATGG GCAGCCCTGC TCCTTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326893.1
CDS	248..5536
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASDSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015471407.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471407.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGATTCCA AATCTATGCA AGATGGCTGG 
GGGGAGAATG ACGGGCCAGT GGCAGGAACT CGCCATCCCA GCTGGGAAGA GGAGGATGAT 
GGAGGAGTCT GGAACACTGC CGGCTCTCAG GGAAGCGCTT CCTCCCACAA CTCAGCAAGC 
TGGGGACAAG GAGGAAAGAA ACAAATGAAG TGCTCACTCA AAGGAGGAAA CAGTGATTCA 
TGGATGAATC CTCTTGCCAA ACAGTTTTCA AATATGGGAT TGCTGAGTCA AACTGAAGAT 
AACCCAAGTA GCAAAATGGA TTTGTCTGTA GGGAGCCTTC CAGATAAAAA ATTTGATGTG 
GACAAGCGAG CAATGAATCT CGGGGATTTT AATGATATCA TGAGGAAGGA TCGATCTGGG 
TTCCGTCCAC CTAATTCCAA AGACATGGGA ACCACAGATA GTGGGCCTTA TTTTGAGAAG 
GGTGGCAATC ATGGTTTGTT TGGAAATAGC ACAGCACAAT CGAGAGGCGT GCACACCCCC 
GTGCAGCCAC TGAACTCTTC TCCTAATCTC CGGGCGCAAG TGCCTCCCCA GTTCATTTCC 
CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG TTTCCCAACA GTGGCCTGAA TCCAGGTCTT 
TTCAACGTGG GGCCCCAGTT ATCTCCTCAA CAAATTGCCA TGCTGAGCCA GCTTCCACAG 
ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT CTCCTGCAGC AGCAGCAACA GCAGCAGTTG 
TTACAAAACC AGAGGAAGCT TTCTCAAGCT GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT 
CGGATGGTGA GCGCCCTCCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGAGGCAG 
CCCAGCATGA AGCATTCGCC ATCTCACCCC GTTGGGCCCA AGCCACATCT GGACAACATG 
GTACCCAACG CTCTGAATGT GGGGCTCCCA GACCTTCAAA CCAAAGGGCC AATACCTGGA 
TATGGTTCTG GCTTCAGCTC CGGCGGCATG GACTACGGCA TGGTTGGTGG GAAGGAGGCT 
GGAACAGAGT CTCGCTTTAA ACAGTGGACC TCCATGATGG AGGTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG 
TCACCATACA ACCAGTTTGA CATCATCCCA GGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCT 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TGCTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTTCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACAGCGCT GCCCCGCCCG CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGCTCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTCGCCTCCG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACCTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACTGATGAC GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCGGCAAC CCCGAGCGCG CCCGCCGCGG GCTGGCAGTC CCTGGAGACC 
GGCCAGACCC AGTCAGAGCC CGTCGGACCT GCTCTGAATC TTTTCGGTGG GTCCACGGGG 
CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC CGGCGCTTCA 
TTGTGGGGCC CCCCCAACTA CTCTTCTAGC TTGTGGGGAG TCCCGACCGT GGAAGACCCT 
CATAGGATGG GCAGCCCTGC TCCTTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015471405.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326891.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

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CloneID	OBa51515
Clone ID Related Accession (Same CDS sequence)	XM_015471405.1
Accession Version	XM_015471405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC ATGGCAAGAC TCCACCTCCT GGTGAAGAAA GCAAACAAGA GAAGGAGCAC 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAGCGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCACC GTCCTGCATG CTCCTCGGGG GCGGGGCAGG GCCTCCTCCC 
TCCACGGCGC CCGGAGCAAA CCCCAACAAC GCACAAGTGA CAGGAGCGCT GCTGCAGAGC 
GAGGGTGGGA CCGTGCCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AGCGGCGCCT CCCCCAACCC CATTCACATC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC ATGGAAGAGT GGCCTTGCAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCAAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAGGTT CCGGGAACGA ATGTAGTCTG GGGGTCTGGA AATCTGACCC TAAGGCTAGA 
TCTGTTCAAT CTTCCAACTC TACCACGGAG AGCAACAGTG GACTAGGAAA CTGGAGGAAT 
GTGAGTGGTC AGGAGAGGGT TGGACCTGGC CCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAGGG GGCATTGGAA 
ACGGAGACGA GTAATTCCAG TGCACAGGTT AGCACAGTGG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA GCAGGCTCAA 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTC AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGGATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAGGAGAG AGAGGACTCC TGGAAAGGAG CTCCTGTTCA GAAATCAACT 
GGGTCAAAAA GTGACTCTTG GGACAACAAT AACAGGTCTA CGGGTGGGTC CTGGAACTTT 
GGCCCTCAGG ACTCTAATGA CAACAAATGG GGTGAAGGGA ACAAAATGAC ATCCGGGGTC 
TCTCAGGGAG AATGGAAACA GCCGACTGGG TCTGACGAGT TCAAAATTGG AGAATGGAGT 
GGTCCAAACC AACCAAATTC TAGCACTGGA GCATGGGACA ATCAGAAGGG CCACCCCCTC 
CCTGAAAACC AAGGCAATGC CCAGGCTCCC TGTTGGGGAA GATCTTCCAG CTCCACGGGA 
AGTGAAGTTG GAGGTCAAAG CACTGGAAGC AACCACAAAG CAGGAAGTAG TGACAGTCAC 
AATTCTGGCC GTCGGTCGTA CCGGCCCACA CATCCTGATT GTCAGGCTGT CTTGCAGACT 
CTTTTGAGCC GAACTGATTT GGACCCCAGG GTGCTCTCAA ACACTGGCTG GGGCCAAACT 
CAAATTAAGC AGGACACAGT GTGGGACGTT GAAGAGGTGC CAAGGCCCGA GGGGAAATCT 
GACAAAGGAA CTGAGGGGTG GGAGAGCGCT GCCACACAGA CCAAGAGCTC AGGGGGCTGG 
GGAGACGCAC CCAGCCAAAG CAATCAAATG AAGTCTGGAT GGGGAGAGCT CTCAGCCTCT 
ACAGAGTGGA AAGACCCCAA GAACACAGGA GGCTGGAATG ACTATAAGAA CAACAACTCT 
TCCAACTGGG GAGGAGGACG ACCGGACGAG AAGACATCTT CCTCTTGGAA TGAGAACTCC 
AGCAAGGATC AGGGGTGGGG AGGTGGACGC CAGCCCAACC AAGGATGGAC TTCTGGAAAG 
AATGGTTGGG GAGAGGAGGT AGATCAGGCG AAAACCAGCA ATTGGGAAAA TGCTGCAAGT 
AAACCTGTGT CCGGGTGGGG CGAAGGTGGG CAGAGTGAAA TCGGGACTTG GGGGAGTGGT 
GGGAACGCGA GCCTGGCTTC CAAAGGCGGG TGGGAAGACT GCAAAAGGTC TCCGGCATGG 
ACGGAGACAG GCAGACAGCC CAGTTCCTGG AACAAACAGC ACCAACAGCA GCCGCCGCCA 
CAGCAGCTAC CACCACCACA ACCCGAGGCC TCTGGCTCCT GGGGAGGCCC AGCCCCACCA 
CCTCCAGGCA ACGGACGACC TTCCAATTCC AACTGGAGCA GCGGGCCACA GCCAGTAACC 
CCCAAGGATG AGGAACCCAG CGGTTGGGAA GAGCCATCCC CACAGTCGAT CAGTCGGAAG 
ATGGACATTG ACGATGGCAC TTCCGCGTGG GGAGACCCCA ACAGTTATAA CTACAAGAAT 
GTGAATCTGT GGGATAAGAA TTCCCAAGGG GGCCCAGCAC CTCGAGAAGC AAACCTGCCT 
ACCCCGATGA CCGGCAAATC AGCATCAGTC TGGAGCAAAA GCACACCATC TGCTCCAGAT 
AATGGTACTT CAGCTTGGGG TGAGCCAAAC GAAAGCAGTC CTGGGTGGGG CGAGATGGAT 
GACACGGGAG CGTCGACAAC AGGCTGGGGG ACTGCACCCT CCAATGCTCC AAATGCCATG 
AAACCTAATT CCAAATCTAT GCAAGATGGC TGGGGGGAGA ATGACGGGCC AGTGGCAGGA 
ACTCGCCATC CCAGCTGGGA AGAGGAGGAT GATGGAGGAG TCTGGAACAC TGCCGGCTCT 
CAGGGAAGCG CTTCCTCCCA CAACTCAGCA AGCTGGGGAC AAGGAGGAAA GAAACAAATG 
AAGTGCTCAC TCAAAGGAGG AAACAGTGAT TCATGGATGA ATCCTCTTGC CAAACAGTTT 
TCAAATATGG GATTGCTGAG TCAAACTGAA GATAACCCAA GTAGCAAAAT GGATTTGTCT 
GTAGGGAGCC TTCCAGATAA AAAATTTGAT GTGGACAAGC GAGCAATGAA TCTCGGGGAT 
TTTAATGATA TCATGAGGAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG CGTGCACACC CCCGTGCAGC CACTGAACTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTCATT TCCCCCCAGG TTTCTGCCTC AATGCTTAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAACG TGGGGCCCCA GTTATCTCCT 
CAACAAATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGTTACAAA ACCAGAGGAA GCTTTCTCAA 
GCTGTTCGCC AGCAGCAGGA GCAGCAGCTG GCTCGGATGG TGAGCGCCCT CCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGAGG CAGCCCAGCA TGAAGCATTC GCCATCTCAC 
CCCGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCCGGCGGC 
ATGGACTACG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGTGCC CTCTGTAGCC ACACAGGAAG CCAATATGCA CAAAAATGGC 
GCTATTGTGG CCCCTGGTAA GACCCGAGGA GGGTCACCAT ACAACCAGTT TGACATCATC 
CCAGGTGACA CACTGGGTGG CCATACGGGT CCTGCTGGTG ATAGCTGGTT ACCTGCCAAA 
TCTCCACCAA CAAATAAAAT CGGAAGTAAA TCTAGCAATG CCAGTTGGCC TCCAGAATTC 
CAACCAGGAG TGCCATGGAA AGGTATCCAA AACATTGACC CTGAATCTGA CCCCTATGTC 
ACCCCAGGAA GTGTGCTGGG GGGTACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ATAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAC TCCTTTACCA ACGTTCATAG CACTTCAGCA 
AAGTTTCCTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA CCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGC GCTGCCCCGC 
CCGCCTCCCG GTCTGACCAA CCCCAAACCA TCATCTCCCT GGAGCAGCAC AGCACCCCGC 
TCAGTCAGGG GGTGGGGGAC ACAGGACTCA CGGCTCGCCT CCGCCTCTAC CTGGAGTGAT 
GGTGGTTCAG TTCGTCCTAG TTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATTGAT 
GGGTCAACCT TGAGAACCAT CTGCATGCAG CATGGCCCAC TGCTGACATT CCATCTGAAC 
CTAACCCAGG GCACTGCCCT GATCCGATAC AGCACCAAAC AGGAGGCGGC CAAGGCCCAA 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TGGCTGAGTT TGCCACTGAT 
GACGAAGTCA GCCGCTTTCT GGCACAAGCT CAGCCCCCTA CACCTGCGGC AACCCCGAGC 
GCGCCCGCCG CGGGCTGGCA GTCCCTGGAG ACCGGCCAGA CCCAGTCAGA GCCCGTCGGA 
CCTGCTCTGA ATCTTTTCGG TGGGTCCACG GGGCTCGGGC AGTGGAGCAG CAGTGCTGGT 
GGCAGCAGCG GGGCCGATCT TGCCGGCGCT TCATTGTGGG GCCCCCCCAA CTACTCTTCT 
AGCTTGTGGG GAGTCCCGAC CGTGGAAGAC CCTCATAGGA TGGGCAGCCC TGCTCCTTTA 
CTACCTGGTG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326891.1
CDS	172..5553
Translation	MEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015471405.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471405.1

ORF Insert Sequence:

ATGGAAGGGC ATGGCAAGAC TCCACCTCCT GGTGAAGAAA GCAAACAAGA GAAGGAGCAC 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAGCGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCACC GTCCTGCATG CTCCTCGGGG GCGGGGCAGG GCCTCCTCCC 
TCCACGGCGC CCGGAGCAAA CCCCAACAAC GCACAAGTGA CAGGAGCGCT GCTGCAGAGC 
GAGGGTGGGA CCGTGCCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AGCGGCGCCT CCCCCAACCC CATTCACATC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC ATGGAAGAGT GGCCTTGCAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCAAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAGGTT CCGGGAACGA ATGTAGTCTG GGGGTCTGGA AATCTGACCC TAAGGCTAGA 
TCTGTTCAAT CTTCCAACTC TACCACGGAG AGCAACAGTG GACTAGGAAA CTGGAGGAAT 
GTGAGTGGTC AGGAGAGGGT TGGACCTGGC CCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAGGG GGCATTGGAA 
ACGGAGACGA GTAATTCCAG TGCACAGGTT AGCACAGTGG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA GCAGGCTCAA 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTC AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGGATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAGGAGAG AGAGGACTCC TGGAAAGGAG CTCCTGTTCA GAAATCAACT 
GGGTCAAAAA GTGACTCTTG GGACAACAAT AACAGGTCTA CGGGTGGGTC CTGGAACTTT 
GGCCCTCAGG ACTCTAATGA CAACAAATGG GGTGAAGGGA ACAAAATGAC ATCCGGGGTC 
TCTCAGGGAG AATGGAAACA GCCGACTGGG TCTGACGAGT TCAAAATTGG AGAATGGAGT 
GGTCCAAACC AACCAAATTC TAGCACTGGA GCATGGGACA ATCAGAAGGG CCACCCCCTC 
CCTGAAAACC AAGGCAATGC CCAGGCTCCC TGTTGGGGAA GATCTTCCAG CTCCACGGGA 
AGTGAAGTTG GAGGTCAAAG CACTGGAAGC AACCACAAAG CAGGAAGTAG TGACAGTCAC 
AATTCTGGCC GTCGGTCGTA CCGGCCCACA CATCCTGATT GTCAGGCTGT CTTGCAGACT 
CTTTTGAGCC GAACTGATTT GGACCCCAGG GTGCTCTCAA ACACTGGCTG GGGCCAAACT 
CAAATTAAGC AGGACACAGT GTGGGACGTT GAAGAGGTGC CAAGGCCCGA GGGGAAATCT 
GACAAAGGAA CTGAGGGGTG GGAGAGCGCT GCCACACAGA CCAAGAGCTC AGGGGGCTGG 
GGAGACGCAC CCAGCCAAAG CAATCAAATG AAGTCTGGAT GGGGAGAGCT CTCAGCCTCT 
ACAGAGTGGA AAGACCCCAA GAACACAGGA GGCTGGAATG ACTATAAGAA CAACAACTCT 
TCCAACTGGG GAGGAGGACG ACCGGACGAG AAGACATCTT CCTCTTGGAA TGAGAACTCC 
AGCAAGGATC AGGGGTGGGG AGGTGGACGC CAGCCCAACC AAGGATGGAC TTCTGGAAAG 
AATGGTTGGG GAGAGGAGGT AGATCAGGCG AAAACCAGCA ATTGGGAAAA TGCTGCAAGT 
AAACCTGTGT CCGGGTGGGG CGAAGGTGGG CAGAGTGAAA TCGGGACTTG GGGGAGTGGT 
GGGAACGCGA GCCTGGCTTC CAAAGGCGGG TGGGAAGACT GCAAAAGGTC TCCGGCATGG 
ACGGAGACAG GCAGACAGCC CAGTTCCTGG AACAAACAGC ACCAACAGCA GCCGCCGCCA 
CAGCAGCTAC CACCACCACA ACCCGAGGCC TCTGGCTCCT GGGGAGGCCC AGCCCCACCA 
CCTCCAGGCA ACGGACGACC TTCCAATTCC AACTGGAGCA GCGGGCCACA GCCAGTAACC 
CCCAAGGATG AGGAACCCAG CGGTTGGGAA GAGCCATCCC CACAGTCGAT CAGTCGGAAG 
ATGGACATTG ACGATGGCAC TTCCGCGTGG GGAGACCCCA ACAGTTATAA CTACAAGAAT 
GTGAATCTGT GGGATAAGAA TTCCCAAGGG GGCCCAGCAC CTCGAGAAGC AAACCTGCCT 
ACCCCGATGA CCGGCAAATC AGCATCAGTC TGGAGCAAAA GCACACCATC TGCTCCAGAT 
AATGGTACTT CAGCTTGGGG TGAGCCAAAC GAAAGCAGTC CTGGGTGGGG CGAGATGGAT 
GACACGGGAG CGTCGACAAC AGGCTGGGGG ACTGCACCCT CCAATGCTCC AAATGCCATG 
AAACCTAATT CCAAATCTAT GCAAGATGGC TGGGGGGAGA ATGACGGGCC AGTGGCAGGA 
ACTCGCCATC CCAGCTGGGA AGAGGAGGAT GATGGAGGAG TCTGGAACAC TGCCGGCTCT 
CAGGGAAGCG CTTCCTCCCA CAACTCAGCA AGCTGGGGAC AAGGAGGAAA GAAACAAATG 
AAGTGCTCAC TCAAAGGAGG AAACAGTGAT TCATGGATGA ATCCTCTTGC CAAACAGTTT 
TCAAATATGG GATTGCTGAG TCAAACTGAA GATAACCCAA GTAGCAAAAT GGATTTGTCT 
GTAGGGAGCC TTCCAGATAA AAAATTTGAT GTGGACAAGC GAGCAATGAA TCTCGGGGAT 
TTTAATGATA TCATGAGGAA GGATCGATCT GGGTTCCGTC CACCTAATTC CAAAGACATG 
GGAACCACAG ATAGTGGGCC TTATTTTGAG AAGGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG CGTGCACACC CCCGTGCAGC CACTGAACTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTCATT TCCCCCCAGG TTTCTGCCTC AATGCTTAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAACG TGGGGCCCCA GTTATCTCCT 
CAACAAATTG CCATGCTGAG CCAGCTTCCA CAGATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGTTACAAA ACCAGAGGAA GCTTTCTCAA 
GCTGTTCGCC AGCAGCAGGA GCAGCAGCTG GCTCGGATGG TGAGCGCCCT CCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGAGG CAGCCCAGCA TGAAGCATTC GCCATCTCAC 
CCCGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCCGGCGGC 
ATGGACTACG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGTGCC CTCTGTAGCC ACACAGGAAG CCAATATGCA CAAAAATGGC 
GCTATTGTGG CCCCTGGTAA GACCCGAGGA GGGTCACCAT ACAACCAGTT TGACATCATC 
CCAGGTGACA CACTGGGTGG CCATACGGGT CCTGCTGGTG ATAGCTGGTT ACCTGCCAAA 
TCTCCACCAA CAAATAAAAT CGGAAGTAAA TCTAGCAATG CCAGTTGGCC TCCAGAATTC 
CAACCAGGAG TGCCATGGAA AGGTATCCAA AACATTGACC CTGAATCTGA CCCCTATGTC 
ACCCCAGGAA GTGTGCTGGG GGGTACAGCC ACATCTCCCA TTGTAGATAC TGACCACCAA 
CTTCTGCGGG ATAACACCAC AGGGTCTAAT TCTTCCCTCA ACACCTCGCT GCCTTCACCT 
GGTGCCTGGC CCTACAGTGC CTCTGACAAC TCCTTTACCA ACGTTCATAG CACTTCAGCA 
AAGTTTCCTG ATTACAAATC AACATGGTCC CCAGATCCCA TAGGACACAA CCCCACTCAT 
CTCTCCAACA AGATGTGGAA AAACCATATT TCCTCAAGGA ACACTACAGC GCTGCCCCGC 
CCGCCTCCCG GTCTGACCAA CCCCAAACCA TCATCTCCCT GGAGCAGCAC AGCACCCCGC 
TCAGTCAGGG GGTGGGGGAC ACAGGACTCA CGGCTCGCCT CCGCCTCTAC CTGGAGTGAT 
GGTGGTTCAG TTCGTCCTAG TTACTGGCTG GTTCTTCACA ATCTCACTCC ACAGATTGAT 
GGGTCAACCT TGAGAACCAT CTGCATGCAG CATGGCCCAC TGCTGACATT CCATCTGAAC 
CTAACCCAGG GCACTGCCCT GATCCGATAC AGCACCAAAC AGGAGGCGGC CAAGGCCCAA 
ACTGCACTGC ACATGTGTGT GTTGGGAAAC ACTACCATCC TGGCTGAGTT TGCCACTGAT 
GACGAAGTCA GCCGCTTTCT GGCACAAGCT CAGCCCCCTA CACCTGCGGC AACCCCGAGC 
GCGCCCGCCG CGGGCTGGCA GTCCCTGGAG ACCGGCCAGA CCCAGTCAGA GCCCGTCGGA 
CCTGCTCTGA ATCTTTTCGG TGGGTCCACG GGGCTCGGGC AGTGGAGCAG CAGTGCTGGT 
GGCAGCAGCG GGGCCGATCT TGCCGGCGCT TCATTGTGGG GCCCCCCCAA CTACTCTTCT 
AGCTTGTGGG GAGTCCCGAC CGTGGAAGAC CCTCATAGGA TGGGCAGCCC TGCTCCTTTA 
CTACCTGGTG ACCTTCTGGG AGGAGGGTCG GATTCAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_010805755.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804057.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

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CloneID	OBa51516
Clone ID Related Accession (Same CDS sequence)	XM_010805755.2
Accession Version	XM_010805755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GTGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCCC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GGGTGGCAAT 
CATGGTTTGT TTGGAAATAG CACAGCACAA TCGAGAGGCG TGCACACCCC CGTGCAGCCA 
CTGAACTCTT CTCCTAATCT CCGGGCGCAA GTGCCTCCCC AGTTCATTTC CCCCCAGGTT 
TCTGCCTCAA TGCTTAAGCA GTTTCCCAAC AGTGGCCTGA ATCCAGGTCT TTTCAACGTG 
GGGCCCCAGT TATCTCCTCA ACAAATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAGTTGG CATGTCAGCT TCTCCTGCAG CAGCAGCAAC AGCAGCAGTT GTTACAAAAC 
CAGAGGAAGC TTTCTCAAGC TGTTCGCCAG CAGCAGGAGC AGCAGCTGGC TCGGATGGTG 
AGCGCCCTCC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGAGGCA GCCCAGCATG 
AAGCATTCGC CATCTCACCC CGTTGGGCCC AAGCCACATC TGGACAACAT GGTACCCAAC 
GCTCTGAATG TGGGGCTCCC AGACCTTCAA ACCAAAGGGC CAATACCTGG ATATGGTTCT 
GGCTTCAGCT CCGGCGGCAT GGACTACGGC ATGGTTGGTG GGAAGGAGGC TGGAACAGAG 
TCTCGCTTTA AACAGTGGAC CTCCATGATG GAGGTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CCCGAGGAGG GTCACCATAC 
AACCAGTTTG ACATCATCCC AGGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC TAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTGCTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTTCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACAGCGC TGCCCCGCCC GCCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGCTC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTCGCCTCC 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCTAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACCTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACTGATGA CGAAGTCAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCGGCAA CCCCGAGCGC GCCCGCCGCG GGCTGGCAGT CCCTGGAGAC CGGCCAGACC 
CAGTCAGAGC CCGTCGGACC TGCTCTGAAT CTTTTCGGTG GGTCCACGGG GCTCGGGCAG 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CCGGCGCTTC ATTGTGGGGC 
CCCCCCAACT ACTCTTCTAG CTTGTGGGGA GTCCCGACCG TGGAAGACCC TCATAGGATG 
GGCAGCCCTG CTCCTTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804057.1
CDS	814..6153
Translation	MREKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_010805755.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805755.2

ORF Insert Sequence:

ATGAGAGAGA AGGAGCACGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA GCGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCACCGT CCTGCATGCT CCTCGGGGGC 
GGGGCAGGGC CTCCTCCCTC CACGGCGCCC GGAGCAAACC CCAACAACGC ACAAGTGACA 
GGAGCGCTGC TGCAGAGCGA GGGTGGGACC GTGCCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAG CGGCGCCTCC 
CCCAACCCCA TTCACATCTG GGACAAGGTG ATTGTAGACG GGTCTGACAT GGAAGAGTGG 
CCTTGCATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCAAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAGGTTCC GGGAACGAAT GTAGTCTGGG GGTCTGGAAA 
TCTGACCCTA AGGCTAGATC TGTTCAATCT TCCAACTCTA CCACGGAGAG CAACAGTGGA 
CTAGGAAACT GGAGGAATGT GAGTGGTCAG GAGAGGGTTG GACCTGGCCC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAGGGGG CATTGGAAAC GGAGACGAGT AATTCCAGTG CACAGGTTAG CACAGTGGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAGC AGGCTCAAAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTCAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG GATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAGGAGAGAG AGGACTCCTG GAAAGGAGCT 
CCTGTTCAGA AATCAACTGG GTCAAAAAGT GACTCTTGGG ACAACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CCGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGACGAGTTC 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAGAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACGGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCACAA TTCTGGCCGT CGGTCGTACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TGCAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACAGTGT GGGACGTTGA AGAGGTGCCA 
AGGCCCGAGG GGAAATCTGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAGCTCAG GGGGCTGGGG AGACGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGAGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGAGGACGAC CGGACGAGAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GTGGACGCCA GCCCAACCAA 
GGATGGACTT CTGGAAAGAA TGGTTGGGGA GAGGAGGTAG ATCAGGCGAA AACCAGCAAT 
TGGGAAAATG CTGCAAGTAA ACCTGTGTCC GGGTGGGGCG AAGGTGGGCA GAGTGAAATC 
GGGACTTGGG GGAGTGGTGG GAACGCGAGC CTGGCTTCCA AAGGCGGGTG GGAAGACTGC 
AAAAGGTCTC CGGCATGGAC GGAGACAGGC AGACAGCCCA GTTCCTGGAA CAAACAGCAC 
CAACAGCAGC CGCCGCCACA GCAGCTACCA CCACCACAAC CCGAGGCCTC TGGCTCCTGG 
GGAGGCCCAG CCCCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGC 
GGGCCACAGC CAGTAACCCC CAAGGATGAG GAACCCAGCG GTTGGGAAGA GCCATCCCCA 
CAGTCGATCA GTCGGAAGAT GGACATTGAC GATGGCACTT CCGCGTGGGG AGACCCCAAC 
AGTTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAAGCAA ACCTGCCTAC CCCGATGACC GGCAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCATCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AGATGGATGA CACGGGAGCG TCGACAACAG GCTGGGGGAC TGCACCCTCC 
AATGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGATGGCTG GGGGGAGAAT 
GACGGGCCAG TGGCAGGAAC TCGCCATCCC AGCTGGGAAG AGGAGGATGA TGGAGGAGTC 
TGGAACACTG CCGGCTCTCA GGGAAGCGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AACAAATGAA GTGCTCACTC AAAGGAGGAA ACAGTGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAACCCAAGT 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGATGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTTGAGAA GGGTGGCAAT 
CATGGTTTGT TTGGAAATAG CACAGCACAA TCGAGAGGCG TGCACACCCC CGTGCAGCCA 
CTGAACTCTT CTCCTAATCT CCGGGCGCAA GTGCCTCCCC AGTTCATTTC CCCCCAGGTT 
TCTGCCTCAA TGCTTAAGCA GTTTCCCAAC AGTGGCCTGA ATCCAGGTCT TTTCAACGTG 
GGGCCCCAGT TATCTCCTCA ACAAATTGCC ATGCTGAGCC AGCTTCCACA GATTCCCCAG 
TTTCAGTTGG CATGTCAGCT TCTCCTGCAG CAGCAGCAAC AGCAGCAGTT GTTACAAAAC 
CAGAGGAAGC TTTCTCAAGC TGTTCGCCAG CAGCAGGAGC AGCAGCTGGC TCGGATGGTG 
AGCGCCCTCC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGAGGCA GCCCAGCATG 
AAGCATTCGC CATCTCACCC CGTTGGGCCC AAGCCACATC TGGACAACAT GGTACCCAAC 
GCTCTGAATG TGGGGCTCCC AGACCTTCAA ACCAAAGGGC CAATACCTGG ATATGGTTCT 
GGCTTCAGCT CCGGCGGCAT GGACTACGGC ATGGTTGGTG GGAAGGAGGC TGGAACAGAG 
TCTCGCTTTA AACAGTGGAC CTCCATGATG GAGGTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CCCGAGGAGG GTCACCATAC 
AACCAGTTTG ACATCATCCC AGGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC TAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTGCTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTTCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACAGCGC TGCCCCGCCC GCCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGCTC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTCGCCTCC 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCTAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACCTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACTGATGA CGAAGTCAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCGGCAA CCCCGAGCGC GCCCGCCGCG GGCTGGCAGT CCCTGGAGAC CGGCCAGACC 
CAGTCAGAGC CCGTCGGACC TGCTCTGAAT CTTTTCGGTG GGTCCACGGG GCTCGGGCAG 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CCGGCGCTTC ATTGTGGGGC 
CCCCCCAACT ACTCTTCTAG CTTGTGGGGA GTCCCGACCG TGGAAGACCC TCATAGGATG 
GGCAGCCCTG CTCCTTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015471406.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326892.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OBa90831
Clone ID Related Accession (Same CDS sequence)	XM_015471406.1
Accession Version	XM_015471406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGA GTCAAACTGA AGATAACCCA AGTAGCAAAA TGGATTTGTC TGTAGGGAGC 
CTTCCAGATA AAAAATTTGA TGTGGACAAG CGAGCAATGA ATCTCGGGGA TTTTAATGAT 
ATCATGAGGA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGGGTGGC AATCATGGTT TGTTTGGAAA TAGCACAGCA 
CAATCGAGAG GCGTGCACAC CCCCGTGCAG CCACTGAACT CTTCTCCTAA TCTCCGGGCG 
CAAGTGCCTC CCCAGTTCAT TTCCCCCCAG GTTTCTGCCT CAATGCTTAA GCAGTTTCCC 
AACAGTGGCC TGAATCCAGG TCTTTTCAAC GTGGGGCCCC AGTTATCTCC TCAACAAATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAGT TGGCATGTCA GCTTCTCCTG 
CAGCAGCAGC AACAGCAGCA GTTGTTACAA AACCAGAGGA AGCTTTCTCA AGCTGTTCGC 
CAGCAGCAGG AGCAGCAGCT GGCTCGGATG GTGAGCGCCC TCCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGAG GCAGCCCAGC ATGAAGCATT CGCCATCTCA CCCCGTTGGG 
CCCAAGCCAC ATCTGGACAA CATGGTACCC AACGCTCTGA ATGTGGGGCT CCCAGACCTT 
CAAACCAAAG GGCCAATACC TGGATATGGT TCTGGCTTCA GCTCCGGCGG CATGGACTAC 
GGCATGGTTG GTGGGAAGGA GGCTGGAACA GAGTCTCGCT TTAAACAGTG GACCTCCATG 
ATGGAGGTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACCCGAGG AGGGTCACCA TACAACCAGT TTGACATCAT CCCAGGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCTAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTGCTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTTCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAG CGCTGCCCCG CCCGCCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG CTCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTCGCC TCCGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCTA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACC 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACTGA TGACGAAGTC 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCGG CAACCCCGAG CGCGCCCGCC 
GCGGGCTGGC AGTCCCTGGA GACCGGCCAG ACCCAGTCAG AGCCCGTCGG ACCTGCTCTG 
AATCTTTTCG GTGGGTCCAC GGGGCTCGGG CAGTGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCCGGCGC TTCATTGTGG GGCCCCCCCA ACTACTCTTC TAGCTTGTGG 
GGAGTCCCGA CCGTGGAAGA CCCTCATAGG ATGGGCAGCC CTGCTCCTTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326892.1
CDS	248..5620
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015471406.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471406.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGA GTCAAACTGA AGATAACCCA AGTAGCAAAA TGGATTTGTC TGTAGGGAGC 
CTTCCAGATA AAAAATTTGA TGTGGACAAG CGAGCAATGA ATCTCGGGGA TTTTAATGAT 
ATCATGAGGA AGGATCGATC TGGGTTCCGT CCACCTAATT CCAAAGACAT GGGAACCACA 
GATAGTGGGC CTTATTTTGA GAAGGGTGGC AATCATGGTT TGTTTGGAAA TAGCACAGCA 
CAATCGAGAG GCGTGCACAC CCCCGTGCAG CCACTGAACT CTTCTCCTAA TCTCCGGGCG 
CAAGTGCCTC CCCAGTTCAT TTCCCCCCAG GTTTCTGCCT CAATGCTTAA GCAGTTTCCC 
AACAGTGGCC TGAATCCAGG TCTTTTCAAC GTGGGGCCCC AGTTATCTCC TCAACAAATT 
GCCATGCTGA GCCAGCTTCC ACAGATTCCC CAGTTTCAGT TGGCATGTCA GCTTCTCCTG 
CAGCAGCAGC AACAGCAGCA GTTGTTACAA AACCAGAGGA AGCTTTCTCA AGCTGTTCGC 
CAGCAGCAGG AGCAGCAGCT GGCTCGGATG GTGAGCGCCC TCCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGAG GCAGCCCAGC ATGAAGCATT CGCCATCTCA CCCCGTTGGG 
CCCAAGCCAC ATCTGGACAA CATGGTACCC AACGCTCTGA ATGTGGGGCT CCCAGACCTT 
CAAACCAAAG GGCCAATACC TGGATATGGT TCTGGCTTCA GCTCCGGCGG CATGGACTAC 
GGCATGGTTG GTGGGAAGGA GGCTGGAACA GAGTCTCGCT TTAAACAGTG GACCTCCATG 
ATGGAGGTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACCCGAGG AGGGTCACCA TACAACCAGT TTGACATCAT CCCAGGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCTAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTGCTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTTCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAG CGCTGCCCCG CCCGCCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG CTCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTCGCC TCCGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCTA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACC 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACTGA TGACGAAGTC 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCGG CAACCCCGAG CGCGCCCGCC 
GCGGGCTGGC AGTCCCTGGA GACCGGCCAG ACCCAGTCAG AGCCCGTCGG ACCTGCTCTG 
AATCTTTTCG GTGGGTCCAC GGGGCTCGGG CAGTGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCCGGCGC TTCATTGTGG GGCCCCCCCA ACTACTCTTC TAGCTTGTGG 
GGAGTCCCGA CCGTGGAAGA CCCTCATAGG ATGGGCAGCC CTGCTCCTTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_015471408.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326894.1 Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OBa90832
Clone ID Related Accession (Same CDS sequence)	XM_015471408.1
Accession Version	XM_015471408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGATGGGT GCCTTGGGGA TGAGGCTCCC TGCTCTCCTT TCTCCCCTTC TCCCAGCTAC 
AAGCTGTCTC CCTCTGGTTC CACACTACCC AACGTCAGCC TTGGAGCAAT CGGCACAGGG 
CTCAACCCCC AAAACTTCGC TGCTAGACAA GGTGGCAATC ATGGTTTGTT TGGAAATAGC 
ACAGCACAAT CGAGAGGCGT GCACACCCCC GTGCAGCCAC TGAACTCTTC TCCTAATCTC 
CGGGCGCAAG TGCCTCCCCA GTTCATTTCC CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG 
TTTCCCAACA GTGGCCTGAA TCCAGGTCTT TTCAACGTGG GGCCCCAGTT ATCTCCTCAA 
CAAATTGCCA TGCTGAGCCA GCTTCCACAG ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT 
CTCCTGCAGC AGCAGCAACA GCAGCAGTTG TTACAAAACC AGAGGAAGCT TTCTCAAGCT 
GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT CGGATGGTGA GCGCCCTCCA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGAGGCAG CCCAGCATGA AGCATTCGCC ATCTCACCCC 
GTTGGGCCCA AGCCACATCT GGACAACATG GTACCCAACG CTCTGAATGT GGGGCTCCCA 
GACCTTCAAA CCAAAGGGCC AATACCTGGA TATGGTTCTG GCTTCAGCTC CGGCGGCATG 
GACTACGGCA TGGTTGGTGG GAAGGAGGCT GGAACAGAGT CTCGCTTTAA ACAGTGGACC 
TCCATGATGG AGGTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG TCACCATACA ACCAGTTTGA CATCATCCCA 
GGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCT AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TGCTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTTCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACAGCGCT GCCCCGCCCG 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGCTCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTCGCCTCCG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACCTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACTGATGAC 
GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCGGCAAC CCCGAGCGCG 
CCCGCCGCGG GCTGGCAGTC CCTGGAGACC GGCCAGACCC AGTCAGAGCC CGTCGGACCT 
GCTCTGAATC TTTTCGGTGG GTCCACGGGG CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC CGGCGCTTCA TTGTGGGGCC CCCCCAACTA CTCTTCTAGC 
TTGTGGGGAG TCCCGACCGT GGAAGACCCT CATAGGATGG GCAGCCCTGC TCCTTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326894.1
CDS	248..5866
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEHEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPAAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANPNNAQVTGALLQSEGGTVPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSAGSGNECSLGVWKSDPKARSVQSSNSTTESNSGLGNWRNVSGQERVGPGPGFSNFNPNSNPSAWPALVQEGNSRKGALETETSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNSSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKEREDSWKGAPVQKSTGSKSDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDEFKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDVEEVPRPEGKSDKGTEGWESAATQTKSSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKTSNWENAASKPVSGWGEGGQSEIGTWGSGGNASLASKGGWEDCKRSPAWTETGRQPSSWNKQHQQQPPPQQLPPPQPEASGSWGGPAPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREANLPTPMTGKSASVWSKSTPSAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGTAPSNAPNAMKPNSKSMQDGWGENDGPVAGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNSDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGVHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEVPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTALPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSEPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_015471408.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471408.1

ORF Insert Sequence:

ATGCAGACCA ATGAGGGAGA AGTATCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCACGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAGC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCACCGTCC TGCATGCTCC TCGGGGGCGG GGCAGGGCCT 
CCTCCCTCCA CGGCGCCCGG AGCAAACCCC AACAACGCAC AAGTGACAGG AGCGCTGCTG 
CAGAGCGAGG GTGGGACCGT GCCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAGCG GCGCCTCCCC CAACCCCATT 
CACATCTGGG ACAAGGTGAT TGTAGACGGG TCTGACATGG AAGAGTGGCC TTGCATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCAAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAGGTTCCGG GAACGAATGT AGTCTGGGGG TCTGGAAATC TGACCCTAAG 
GCTAGATCTG TTCAATCTTC CAACTCTACC ACGGAGAGCA ACAGTGGACT AGGAAACTGG 
AGGAATGTGA GTGGTCAGGA GAGGGTTGGA CCTGGCCCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAGGGGGCA 
TTGGAAACGG AGACGAGTAA TTCCAGTGCA CAGGTTAGCA CAGTGGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAGCAG 
GCTCAAATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTCAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGGA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA GGAGAGAGAG GACTCCTGGA AAGGAGCTCC TGTTCAGAAA 
TCAACTGGGT CAAAAAGTGA CTCTTGGGAC AACAATAACA GGTCTACGGG TGGGTCCTGG 
AACTTTGGCC CTCAGGACTC TAATGACAAC AAATGGGGTG AAGGGAACAA AATGACATCC 
GGGGTCTCTC AGGGAGAATG GAAACAGCCG ACTGGGTCTG ACGAGTTCAA AATTGGAGAA 
TGGAGTGGTC CAAACCAACC AAATTCTAGC ACTGGAGCAT GGGACAATCA GAAGGGCCAC 
CCCCTCCCTG AAAACCAAGG CAATGCCCAG GCTCCCTGTT GGGGAAGATC TTCCAGCTCC 
ACGGGAAGTG AAGTTGGAGG TCAAAGCACT GGAAGCAACC ACAAAGCAGG AAGTAGTGAC 
AGTCACAATT CTGGCCGTCG GTCGTACCGG CCCACACATC CTGATTGTCA GGCTGTCTTG 
CAGACTCTTT TGAGCCGAAC TGATTTGGAC CCCAGGGTGC TCTCAAACAC TGGCTGGGGC 
CAAACTCAAA TTAAGCAGGA CACAGTGTGG GACGTTGAAG AGGTGCCAAG GCCCGAGGGG 
AAATCTGACA AAGGAACTGA GGGGTGGGAG AGCGCTGCCA CACAGACCAA GAGCTCAGGG 
GGCTGGGGAG ACGCACCCAG CCAAAGCAAT CAAATGAAGT CTGGATGGGG AGAGCTCTCA 
GCCTCTACAG AGTGGAAAGA CCCCAAGAAC ACAGGAGGCT GGAATGACTA TAAGAACAAC 
AACTCTTCCA ACTGGGGAGG AGGACGACCG GACGAGAAGA CATCTTCCTC TTGGAATGAG 
AACTCCAGCA AGGATCAGGG GTGGGGAGGT GGACGCCAGC CCAACCAAGG ATGGACTTCT 
GGAAAGAATG GTTGGGGAGA GGAGGTAGAT CAGGCGAAAA CCAGCAATTG GGAAAATGCT 
GCAAGTAAAC CTGTGTCCGG GTGGGGCGAA GGTGGGCAGA GTGAAATCGG GACTTGGGGG 
AGTGGTGGGA ACGCGAGCCT GGCTTCCAAA GGCGGGTGGG AAGACTGCAA AAGGTCTCCG 
GCATGGACGG AGACAGGCAG ACAGCCCAGT TCCTGGAACA AACAGCACCA ACAGCAGCCG 
CCGCCACAGC AGCTACCACC ACCACAACCC GAGGCCTCTG GCTCCTGGGG AGGCCCAGCC 
CCACCACCTC CAGGCAACGG ACGACCTTCC AATTCCAACT GGAGCAGCGG GCCACAGCCA 
GTAACCCCCA AGGATGAGGA ACCCAGCGGT TGGGAAGAGC CATCCCCACA GTCGATCAGT 
CGGAAGATGG ACATTGACGA TGGCACTTCC GCGTGGGGAG ACCCCAACAG TTATAACTAC 
AAGAATGTGA ATCTGTGGGA TAAGAATTCC CAAGGGGGCC CAGCACCTCG AGAAGCAAAC 
CTGCCTACCC CGATGACCGG CAAATCAGCA TCAGTCTGGA GCAAAAGCAC ACCATCTGCT 
CCAGATAATG GTACTTCAGC TTGGGGTGAG CCAAACGAAA GCAGTCCTGG GTGGGGCGAG 
ATGGATGACA CGGGAGCGTC GACAACAGGC TGGGGGACTG CACCCTCCAA TGCTCCAAAT 
GCCATGAAAC CTAATTCCAA ATCTATGCAA GATGGCTGGG GGGAGAATGA CGGGCCAGTG 
GCAGGAACTC GCCATCCCAG CTGGGAAGAG GAGGATGATG GAGGAGTCTG GAACACTGCC 
GGCTCTCAGG GAAGCGCTTC CTCCCACAAC TCAGCAAGCT GGGGACAAGG AGGAAAGAAA 
CAAATGAAGT GCTCACTCAA AGGAGGAAAC AGTGATTCAT GGATGAATCC TCTTGCCAAA 
CAGTTTTCAA ATATGGGATT GCTGAGTCAA ACTGAAGATA ACCCAAGTAG CAAAATGGAT 
TTGTCTGTAG GGAGCCTTCC AGATAAAAAA TTTGATGTGG ACAAGCGAGC AATGAATCTC 
GGGGATTTTA ATGATATCAT GAGGAAGGAT CGATCTGGGT TCCGTCCACC TAATTCCAAA 
GACATGGGAA CCACAGATAG TGGGCCTTAT TTTGAGAAGC TGACTTTGCC TTTCTCCAAT 
CAAGATGGGT GCCTTGGGGA TGAGGCTCCC TGCTCTCCTT TCTCCCCTTC TCCCAGCTAC 
AAGCTGTCTC CCTCTGGTTC CACACTACCC AACGTCAGCC TTGGAGCAAT CGGCACAGGG 
CTCAACCCCC AAAACTTCGC TGCTAGACAA GGTGGCAATC ATGGTTTGTT TGGAAATAGC 
ACAGCACAAT CGAGAGGCGT GCACACCCCC GTGCAGCCAC TGAACTCTTC TCCTAATCTC 
CGGGCGCAAG TGCCTCCCCA GTTCATTTCC CCCCAGGTTT CTGCCTCAAT GCTTAAGCAG 
TTTCCCAACA GTGGCCTGAA TCCAGGTCTT TTCAACGTGG GGCCCCAGTT ATCTCCTCAA 
CAAATTGCCA TGCTGAGCCA GCTTCCACAG ATTCCCCAGT TTCAGTTGGC ATGTCAGCTT 
CTCCTGCAGC AGCAGCAACA GCAGCAGTTG TTACAAAACC AGAGGAAGCT TTCTCAAGCT 
GTTCGCCAGC AGCAGGAGCA GCAGCTGGCT CGGATGGTGA GCGCCCTCCA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGAGGCAG CCCAGCATGA AGCATTCGCC ATCTCACCCC 
GTTGGGCCCA AGCCACATCT GGACAACATG GTACCCAACG CTCTGAATGT GGGGCTCCCA 
GACCTTCAAA CCAAAGGGCC AATACCTGGA TATGGTTCTG GCTTCAGCTC CGGCGGCATG 
GACTACGGCA TGGTTGGTGG GAAGGAGGCT GGAACAGAGT CTCGCTTTAA ACAGTGGACC 
TCCATGATGG AGGTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC CCGAGGAGGG TCACCATACA ACCAGTTTGA CATCATCCCA 
GGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCT AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TGCTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTTCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACAGCGCT GCCCCGCCCG 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGCTCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTCGCCTCCG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCTAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACCTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACTGATGAC 
GAAGTCAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCGGCAAC CCCGAGCGCG 
CCCGCCGCGG GCTGGCAGTC CCTGGAGACC GGCCAGACCC AGTCAGAGCC CGTCGGACCT 
GCTCTGAATC TTTTCGGTGG GTCCACGGGG CTCGGGCAGT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC CGGCGCTTCA TTGTGGGGCC CCCCCAACTA CTCTTCTAGC 
TTGTGGGGAG TCCCGACCGT GGAAGACCCT CATAGGATGG GCAGCCCTGC TCCTTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNRC6B
Entrez Gene ID	528167
Full Name	trinucleotide repeat containing 6B
General protein information	Preferred Names trinucleotide repeat containing 6B Names trinucleotide repeat-containing gene 6B protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	TNRC6B	XP_001101111.2
Canis lupus familiaris (dog)	TNRC6B	XP_005625909.1
Bos taurus (cattle)	TNRC6B	NP_001179584.1
Gallus gallus (chicken)	TNRC6B	XP_004937779.1
Danio rerio (zebrafish)	tnrc6b	XP_002663990.3
Xenopus tropicalis (tropical clawed frog)	tnrc6b	XP_002934255.2
Homo sapiens (human)	TNRC6B	NP_001155973.1
Pan troglodytes (chimpanzee)	TNRC6B	XP_003317299.1
Mus musculus (house mouse)	Tnrc6b	NP_659061.2
Rattus norvegicus (Norway rat)	Tnrc6b	NP_620200.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNRC6B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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