NLRP1 cDNA ORF clone, Bos taurus(cattle)

The following NLRP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226751 XM_024980720.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226752 XM_024980721.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226753 XM_024980722.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226754 XM_024980725.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226754 XM_024980724.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226754 XM_024980723.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226755 XM_024980726.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa51512 XM_024980719.1
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa10079 XM_003587406.4
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10079 XM_003583564.3
Latest version!
Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51513 XM_010815958.2
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51513 XM_010824036.1
Latest version!
Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51514 XM_010824037.1
Latest version!
Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51514 XM_010815959.2
Latest version!
Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa10079 XM_003587406.3 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51513 XM_010815958.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa51514 XM_010815959.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226751
    Clone ID Related Accession (Same CDS sequence) XM_024980720.1
    Accession Version XM_024980720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGCTGGCAG GCTGCGGCCT CACGTCTGGC
    TGCTGCCAGG ACCTGGCCTC CATGCTCAGG GTCAGCCCCA GCCTGATGGA GCTAAACCTG
    CTGCAGAATG ACCTGGACGA CCTCGGCGTG AGGCTGCTCT GTGAAGGACT CAGGCACCCT
    TCCTGCCAGC TCATATGCCT ATGGCTGGAC CAGACTCAGC TGAGCGAGGA GGTGACCAAG
    ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA
    AGTCTGAGGA TCCTTCACGA GGGCCCAGGT GGAGCAGAGA TGAGTGATAC ATCCTCCCTC
    AAGCGGCAGA GACAAGCATC AGAGGGCAGC TCTCCTCAAG CAGCACAGGT GAGGCTCTTC
    TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC GAGCACATGG AGTCTCTGGG GACCGAGGAT
    GACCTCTGGG GCCCCACAGG GCCTGTGGCT ACCGAGGCGG TTGACGAAGA GAGGAGCCTG
    TACCGCGTTC ACTTGCCCAT GGCCGGCTTC TACCACTGGC CCAACACAGG TCTCCGCTTT
    GAGGTGAGAG GGCCAGTGAC CGTTGAAATT GAATTCTGTG CCTGGGACCA GTTCCTGAAC
    AGGAATTTCC CACAGCGCAG CTGGCTGGCT GCAGGGCCTC TGTTTGACAT CAAGGCTGAA
    CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT CACTTTGTGG CCTTCCGAGG GAACGATGTG
    GACATCTCCC AGTTTCAAGT GGCCCACTTC AAAGAGGAGG GGATGCTCCT GGAGAAGCCA
    GCCAGGGTGA CGCCATGTTA CGCCGTCCTG GAAAACCCCA GCTTCTCGCC CATGGGAGTT
    CTACTGAGGA TGGTCCAAAC TGCCCTGCGC TTCATTCCCA TCACCTCCAC TGTGTTGCTC
    TACCATTACC TCCAGCCTGA AGAAGTTACC TTCCACCTCT ACCTGATCCC CAGCGATTGC
    TCCATTCAGA AGGCCATAGA CAATGAAGAA AAGATGTTCC AGTTTGTGCG AATTCACAAG
    CCACCCCCGC TGACCCCACT CTACATGGGC TCCCGCTACA TTGTGTCTGG TTCAGAGAAG
    CTGGAAATAC TGCCCGAGGA ATTGGAGCTC TGCTATCGGA GCCCCAGAGA ATCTCAGCTG
    TTCTCTGAGT TCTACGTTCG CCATTTGAGG TCAGGGATCA GGTTGCAAAT AAGAAGCAAG
    AAAGATGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT
    ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA CCTGACTCCC CAGCTGCCCT GAGATTGAGG
    CACTTTGTGG ACCGACATCG GGAGCAGCTG GTGGCTCGAG TGACATCCGT GGACCCTATC
    CTGGACAAGT TGCATGGACA GGTGCTGAGC GAAGAACAGT ATGAGGGGGT GCGGGCAGAG
    ACCACTGCAC CAGGCCAGAT GCGGAGGCTG TTTGGTTTCA GCCGATCCTG GGACTGGGCC
    TGCAAAGATC GCCTCTACCA AGCCCTGAAG GAGACCCATC CTCACCTAAT CATGGAACTC
    TGGGAGAAGT GGGGCAGTGA AGAGACCAGG CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836488.1
    CDS477..4538
    Translation

    Target ORF information:

    RefSeq Version XM_024980720.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGCTGGCAG GCTGCGGCCT CACGTCTGGC
    TGCTGCCAGG ACCTGGCCTC CATGCTCAGG GTCAGCCCCA GCCTGATGGA GCTAAACCTG
    CTGCAGAATG ACCTGGACGA CCTCGGCGTG AGGCTGCTCT GTGAAGGACT CAGGCACCCT
    TCCTGCCAGC TCATATGCCT ATGGCTGGAC CAGACTCAGC TGAGCGAGGA GGTGACCAAG
    ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA
    AGTCTGAGGA TCCTTCACGA GGGCCCAGGT GGAGCAGAGA TGAGTGATAC ATCCTCCCTC
    AAGCGGCAGA GACAAGCATC AGAGGGCAGC TCTCCTCAAG CAGCACAGGT GAGGCTCTTC
    TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC GAGCACATGG AGTCTCTGGG GACCGAGGAT
    GACCTCTGGG GCCCCACAGG GCCTGTGGCT ACCGAGGCGG TTGACGAAGA GAGGAGCCTG
    TACCGCGTTC ACTTGCCCAT GGCCGGCTTC TACCACTGGC CCAACACAGG TCTCCGCTTT
    GAGGTGAGAG GGCCAGTGAC CGTTGAAATT GAATTCTGTG CCTGGGACCA GTTCCTGAAC
    AGGAATTTCC CACAGCGCAG CTGGCTGGCT GCAGGGCCTC TGTTTGACAT CAAGGCTGAA
    CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT CACTTTGTGG CCTTCCGAGG GAACGATGTG
    GACATCTCCC AGTTTCAAGT GGCCCACTTC AAAGAGGAGG GGATGCTCCT GGAGAAGCCA
    GCCAGGGTGA CGCCATGTTA CGCCGTCCTG GAAAACCCCA GCTTCTCGCC CATGGGAGTT
    CTACTGAGGA TGGTCCAAAC TGCCCTGCGC TTCATTCCCA TCACCTCCAC TGTGTTGCTC
    TACCATTACC TCCAGCCTGA AGAAGTTACC TTCCACCTCT ACCTGATCCC CAGCGATTGC
    TCCATTCAGA AGGCCATAGA CAATGAAGAA AAGATGTTCC AGTTTGTGCG AATTCACAAG
    CCACCCCCGC TGACCCCACT CTACATGGGC TCCCGCTACA TTGTGTCTGG TTCAGAGAAG
    CTGGAAATAC TGCCCGAGGA ATTGGAGCTC TGCTATCGGA GCCCCAGAGA ATCTCAGCTG
    TTCTCTGAGT TCTACGTTCG CCATTTGAGG TCAGGGATCA GGTTGCAAAT AAGAAGCAAG
    AAAGATGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT
    ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA CCTGACTCCC CAGCTGCCCT GAGATTGAGG
    CACTTTGTGG ACCGACATCG GGAGCAGCTG GTGGCTCGAG TGACATCCGT GGACCCTATC
    CTGGACAAGT TGCATGGACA GGTGCTGAGC GAAGAACAGT ATGAGGGGGT GCGGGCAGAG
    ACCACTGCAC CAGGCCAGAT GCGGAGGCTG TTTGGTTTCA GCCGATCCTG GGACTGGGCC
    TGCAAAGATC GCCTCTACCA AGCCCTGAAG GAGACCCATC CTCACCTAAT CATGGAACTC
    TGGGAGAAGT GGGGCAGTGA AGAGACCAGG CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226752
    Clone ID Related Accession (Same CDS sequence) XM_024980721.1
    Accession Version XM_024980721.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGGAA GCAGACAGAG ATACCAGGAT
    CAAAGGAGAG AGACACCTTG CTCCACCTGG TCATGGAAAA ATGAAGATTT GCACCCAAAA
    TTCATGCAGT TGCTACTTCT ACACACTCCT TACCACAAAG ACTACGAGTC CTTGAACCGG
    GAAAGCTGGG ATCACGGGGT GGTTGAGGGG CAAGGGCATT TGATTGAGGT CAGAGACTTA
    TTTGGCTCAG ACCTGGGTTC CCAGGAAGGG CCTCACACAG TCATATTGCA TGGAGTTGCT
    GGAATTGGGA AGTCCACACT GGCCAGGCAC ATAAGAAGAG CCTGGGAGGA GGGCCAGCTA
    TACAGGAACC ACTTCCAGCA TGTCTTCTAC TTCAACTGCA GAGAGCTGGC CACGTCCAAG
    CTGACAAGTC TGGAGGAACT CATCACAAAA GACCAGTCCA CCACTGTGGC TCCCGCTGGA
    CAGATCCAGT CTCAGCCGGA GCAGCTGCTC TTCATCCTCG ATGGTTTAGA TGAACTGGAA
    TGTGTCTCAG AGGAGAAGAG GGCAGAGCTC TGTCTGCACC GGAGCCAGCA GCAGTCTGTG
    CAGACACTGC TGGGCAGTTT GGTGGAGAAA ACCATATTTC CAGAGGCATC CTTGCTGATC
    ACAGCTCGGA CTGCAGCTCT GCAGAAGTTC ATTCCTTCTT TGAAGCAGCC ATGTTGGGTG
    GAGGTCCTGG GGTTCTCCGA GTCTTCCAGG AAGGACTATT TCTATGAATA TTTCACAGAG
    GAAAGCCAAG CAACTAGAGC CTTTAGCGTG GTTGAATCAA ACCAAGCACT CTTGACCATG
    TGCCTCACGC CCTTGGTGTC CTGGCTGGCC TGCACTTGCC TGAAACAGCA GATGGAGGCA
    GGGGAAGAAC TCTCACTGAA GTCGCAGACC ATGACAGCCC TCTATCTGCG CTACCTTGCC
    CAAGCTATCC AAGCTCAGCC CCTGGGCACT CAGCTGAGGG GCTTCTGTAC TCTTGCTGCT
    GAGGGTATCT GTCAAGGAAA GACTCTGTTC AGTCCAGGGG ACCTCAAGAA GCATGGATTA
    GATGGGGTCA TCAGTGCCAC TCCCTTAAAG ATGGATGTTC TTCAAAAACA CCCCACATCT
    CTGAACTACA GCTTCATTCA CCTGTGTTTC CAGGAGTTCT TTGCAGCAGT GTCCTTTGCC
    TTAGGGGACA TTCGGGACAA AAGTGACCAT CCTAACAGCA CTGGAAGCAT GGAAAAGTTG
    GTAGAAGTAT ATGGGATCAA TAACCTCTTT GGGGCACCAA CCGTGCATTT CCTATCCGGC
    CTTTTGAGTG AGCAAGGAGC GAGAGAGATG GAGAACATTT TCCAATGCAA GCTGTCTTGG
    GAGAGAAAGC GGGAGTCACT GCTGCGGTGG GCTGAATTGG AACTCCGGCG TGAGCATTAC
    TCTCTGCAAT TGTTTCACTG TTTGTACGAG ATTCAGGACG AGGAGTTCCT GACACAAGCG
    ATGGCCCATT TCCAAGGAAT GAGAGTGTGC ATCCGAACCA GCATGGAGCT GCTCGTGTTC
    ACTTTCTGCA TTAGATTCTG CAGCCACGTG TGGCGGCTTC AACTGAATGA GGGCAGACAA
    CATCCAGGAG CATGCAGGCC CACCGACACA GTGCTGTTGA GCTGGGTCCC GGCCACAGAA
    GCCTGCTGGC AGGTTCTCTG CTCTGTTCTC CAGGTCACTG GAAACCTGAA GGAACTGGAC
    CTGAGTAGAA ACATCTTGAG CCGCTCTGCA GTACAGAGTC TTGATGAGGC CCTGAGATGC
    CCTGGGTGCC AGCTAGAGAC CCTGCGGTTG GCCAGCTGCA GCCTCACAGC TGAGAGCTGC
    AAGGACATTG CCTCTGGGCT GAGCACCAGC CAAACCCTGA TGCAATTGGA ATTGAGCTTC
    AACACACTCC TGGACGCAGG AGCTGAGCAC CTTTGCCAGG GCCTGAGACA GCCAACCTGC
    AAGCTGCAGC GACTGTTGCT GGCAGGCTGC GGCCTCACGT CTGGCTGCTG CCAGGACCTG
    GCCTCCATGC TCAGGGTCAG CCCCAGCCTG ATGGAGCTAA ACCTGCTGCA GAATGACCTG
    GACGACCTCG GCGTGAGGCT GCTCTGTGAA GGACTCAGGC ACCCTTCCTG CCAGCTCATA
    TGCCTATGGC TGGACCAGAC TCAGCTGAGC GAGGAGGTGA CCAAGATGCT GAGGGCCCTG
    GAGAGGCAGA AGCCTCAGCT GCTAGTCTCC GGGATATGGA AGCCAAGTCT GAGGATCCTT
    CACGAGGGCC CAGGTGGAGC AGAGATGAGT GATACATCCT CCCTCAAGCG GCAGAGACAA
    GCATCAGAGG GCAGCTCTCC TCAAGCAGCA CAGGTGAGGC TCTTCTGTCC GTCTTCTCCT
    GCCCCTCCGG AGGACGAGCA CATGGAGTCT CTGGGGACCG AGGATGACCT CTGGGGCCCC
    ACAGGGCCTG TGGCTACCGA GGCGGTTGAC GAAGAGAGGA GCCTGTACCG CGTTCACTTG
    CCCATGGCCG GCTTCTACCA CTGGCCCAAC ACAGGTCTCC GCTTTGAGGT GAGAGGGCCA
    GTGACCGTTG AAATTGAATT CTGTGCCTGG GACCAGTTCC TGAACAGGAA TTTCCCACAG
    CGCAGCTGGC TGGCTGCAGG GCCTCTGTTT GACATCAAGG CTGAACCAGG CGCTGTGGCA
    GCCGTGTTCC TCCCTCACTT TGTGGCCTTC CGAGGGAACG ATGTGGACAT CTCCCAGTTT
    CAAGTGGCCC ACTTCAAAGA GGAGGGGATG CTCCTGGAGA AGCCAGCCAG GGTGACGCCA
    TGTTACGCCG TCCTGGAAAA CCCCAGCTTC TCGCCCATGG GAGTTCTACT GAGGATGGTC
    CAAACTGCCC TGCGCTTCAT TCCCATCACC TCCACTGTGT TGCTCTACCA TTACCTCCAG
    CCTGAAGAAG TTACCTTCCA CCTCTACCTG ATCCCCAGCG ATTGCTCCAT TCAGAAGGCC
    ATAGACAATG AAGAAAAGAT GTTCCAGTTT GTGCGAATTC ACAAGCCACC CCCGCTGACC
    CCACTCTACA TGGGCTCCCG CTACATTGTG TCTGGTTCAG AGAAGCTGGA AATACTGCCC
    GAGGAATTGG AGCTCTGCTA TCGGAGCCCC AGAGAATCTC AGCTGTTCTC TGAGTTCTAC
    GTTCGCCATT TGAGGTCAGG GATCAGGTTG CAAATAAGAA GCAAGAAAGA TGGGACTGTG
    GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC AGTCTCTCCA
    GGCCTCAGAG CCTCACCTGA CTCCCCAGCT GCCCTGAGAT TGAGGCACTT TGTGGACCGA
    CATCGGGAGC AGCTGGTGGC TCGAGTGACA TCCGTGGACC CTATCCTGGA CAAGTTGCAT
    GGACAGGTGC TGAGCGAAGA ACAGTATGAG GGGGTGCGGG CAGAGACCAC TGCACCAGGC
    CAGATGCGGA GGCTGTTTGG TTTCAGCCGA TCCTGGGACT GGGCCTGCAA AGATCGCCTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTAATCATGG AACTCTGGGA GAAGTGGGGC
    AGTGAAGAGA CCAGGCCGGG CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836489.1
    CDS478..4344
    Translation

    Target ORF information:

    RefSeq Version XM_024980721.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980721.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGGAA GCAGACAGAG ATACCAGGAT
    CAAAGGAGAG AGACACCTTG CTCCACCTGG TCATGGAAAA ATGAAGATTT GCACCCAAAA
    TTCATGCAGT TGCTACTTCT ACACACTCCT TACCACAAAG ACTACGAGTC CTTGAACCGG
    GAAAGCTGGG ATCACGGGGT GGTTGAGGGG CAAGGGCATT TGATTGAGGT CAGAGACTTA
    TTTGGCTCAG ACCTGGGTTC CCAGGAAGGG CCTCACACAG TCATATTGCA TGGAGTTGCT
    GGAATTGGGA AGTCCACACT GGCCAGGCAC ATAAGAAGAG CCTGGGAGGA GGGCCAGCTA
    TACAGGAACC ACTTCCAGCA TGTCTTCTAC TTCAACTGCA GAGAGCTGGC CACGTCCAAG
    CTGACAAGTC TGGAGGAACT CATCACAAAA GACCAGTCCA CCACTGTGGC TCCCGCTGGA
    CAGATCCAGT CTCAGCCGGA GCAGCTGCTC TTCATCCTCG ATGGTTTAGA TGAACTGGAA
    TGTGTCTCAG AGGAGAAGAG GGCAGAGCTC TGTCTGCACC GGAGCCAGCA GCAGTCTGTG
    CAGACACTGC TGGGCAGTTT GGTGGAGAAA ACCATATTTC CAGAGGCATC CTTGCTGATC
    ACAGCTCGGA CTGCAGCTCT GCAGAAGTTC ATTCCTTCTT TGAAGCAGCC ATGTTGGGTG
    GAGGTCCTGG GGTTCTCCGA GTCTTCCAGG AAGGACTATT TCTATGAATA TTTCACAGAG
    GAAAGCCAAG CAACTAGAGC CTTTAGCGTG GTTGAATCAA ACCAAGCACT CTTGACCATG
    TGCCTCACGC CCTTGGTGTC CTGGCTGGCC TGCACTTGCC TGAAACAGCA GATGGAGGCA
    GGGGAAGAAC TCTCACTGAA GTCGCAGACC ATGACAGCCC TCTATCTGCG CTACCTTGCC
    CAAGCTATCC AAGCTCAGCC CCTGGGCACT CAGCTGAGGG GCTTCTGTAC TCTTGCTGCT
    GAGGGTATCT GTCAAGGAAA GACTCTGTTC AGTCCAGGGG ACCTCAAGAA GCATGGATTA
    GATGGGGTCA TCAGTGCCAC TCCCTTAAAG ATGGATGTTC TTCAAAAACA CCCCACATCT
    CTGAACTACA GCTTCATTCA CCTGTGTTTC CAGGAGTTCT TTGCAGCAGT GTCCTTTGCC
    TTAGGGGACA TTCGGGACAA AAGTGACCAT CCTAACAGCA CTGGAAGCAT GGAAAAGTTG
    GTAGAAGTAT ATGGGATCAA TAACCTCTTT GGGGCACCAA CCGTGCATTT CCTATCCGGC
    CTTTTGAGTG AGCAAGGAGC GAGAGAGATG GAGAACATTT TCCAATGCAA GCTGTCTTGG
    GAGAGAAAGC GGGAGTCACT GCTGCGGTGG GCTGAATTGG AACTCCGGCG TGAGCATTAC
    TCTCTGCAAT TGTTTCACTG TTTGTACGAG ATTCAGGACG AGGAGTTCCT GACACAAGCG
    ATGGCCCATT TCCAAGGAAT GAGAGTGTGC ATCCGAACCA GCATGGAGCT GCTCGTGTTC
    ACTTTCTGCA TTAGATTCTG CAGCCACGTG TGGCGGCTTC AACTGAATGA GGGCAGACAA
    CATCCAGGAG CATGCAGGCC CACCGACACA GTGCTGTTGA GCTGGGTCCC GGCCACAGAA
    GCCTGCTGGC AGGTTCTCTG CTCTGTTCTC CAGGTCACTG GAAACCTGAA GGAACTGGAC
    CTGAGTAGAA ACATCTTGAG CCGCTCTGCA GTACAGAGTC TTGATGAGGC CCTGAGATGC
    CCTGGGTGCC AGCTAGAGAC CCTGCGGTTG GCCAGCTGCA GCCTCACAGC TGAGAGCTGC
    AAGGACATTG CCTCTGGGCT GAGCACCAGC CAAACCCTGA TGCAATTGGA ATTGAGCTTC
    AACACACTCC TGGACGCAGG AGCTGAGCAC CTTTGCCAGG GCCTGAGACA GCCAACCTGC
    AAGCTGCAGC GACTGTTGCT GGCAGGCTGC GGCCTCACGT CTGGCTGCTG CCAGGACCTG
    GCCTCCATGC TCAGGGTCAG CCCCAGCCTG ATGGAGCTAA ACCTGCTGCA GAATGACCTG
    GACGACCTCG GCGTGAGGCT GCTCTGTGAA GGACTCAGGC ACCCTTCCTG CCAGCTCATA
    TGCCTATGGC TGGACCAGAC TCAGCTGAGC GAGGAGGTGA CCAAGATGCT GAGGGCCCTG
    GAGAGGCAGA AGCCTCAGCT GCTAGTCTCC GGGATATGGA AGCCAAGTCT GAGGATCCTT
    CACGAGGGCC CAGGTGGAGC AGAGATGAGT GATACATCCT CCCTCAAGCG GCAGAGACAA
    GCATCAGAGG GCAGCTCTCC TCAAGCAGCA CAGGTGAGGC TCTTCTGTCC GTCTTCTCCT
    GCCCCTCCGG AGGACGAGCA CATGGAGTCT CTGGGGACCG AGGATGACCT CTGGGGCCCC
    ACAGGGCCTG TGGCTACCGA GGCGGTTGAC GAAGAGAGGA GCCTGTACCG CGTTCACTTG
    CCCATGGCCG GCTTCTACCA CTGGCCCAAC ACAGGTCTCC GCTTTGAGGT GAGAGGGCCA
    GTGACCGTTG AAATTGAATT CTGTGCCTGG GACCAGTTCC TGAACAGGAA TTTCCCACAG
    CGCAGCTGGC TGGCTGCAGG GCCTCTGTTT GACATCAAGG CTGAACCAGG CGCTGTGGCA
    GCCGTGTTCC TCCCTCACTT TGTGGCCTTC CGAGGGAACG ATGTGGACAT CTCCCAGTTT
    CAAGTGGCCC ACTTCAAAGA GGAGGGGATG CTCCTGGAGA AGCCAGCCAG GGTGACGCCA
    TGTTACGCCG TCCTGGAAAA CCCCAGCTTC TCGCCCATGG GAGTTCTACT GAGGATGGTC
    CAAACTGCCC TGCGCTTCAT TCCCATCACC TCCACTGTGT TGCTCTACCA TTACCTCCAG
    CCTGAAGAAG TTACCTTCCA CCTCTACCTG ATCCCCAGCG ATTGCTCCAT TCAGAAGGCC
    ATAGACAATG AAGAAAAGAT GTTCCAGTTT GTGCGAATTC ACAAGCCACC CCCGCTGACC
    CCACTCTACA TGGGCTCCCG CTACATTGTG TCTGGTTCAG AGAAGCTGGA AATACTGCCC
    GAGGAATTGG AGCTCTGCTA TCGGAGCCCC AGAGAATCTC AGCTGTTCTC TGAGTTCTAC
    GTTCGCCATT TGAGGTCAGG GATCAGGTTG CAAATAAGAA GCAAGAAAGA TGGGACTGTG
    GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC AGTCTCTCCA
    GGCCTCAGAG CCTCACCTGA CTCCCCAGCT GCCCTGAGAT TGAGGCACTT TGTGGACCGA
    CATCGGGAGC AGCTGGTGGC TCGAGTGACA TCCGTGGACC CTATCCTGGA CAAGTTGCAT
    GGACAGGTGC TGAGCGAAGA ACAGTATGAG GGGGTGCGGG CAGAGACCAC TGCACCAGGC
    CAGATGCGGA GGCTGTTTGG TTTCAGCCGA TCCTGGGACT GGGCCTGCAA AGATCGCCTC
    TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTAATCATGG AACTCTGGGA GAAGTGGGGC
    AGTGAAGAGA CCAGGCCGGG CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226753
    Clone ID Related Accession (Same CDS sequence) XM_024980722.1
    Accession Version XM_024980722.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AGATCTCAGA CGGGCAGTTA CTCCAGTCTC TCCAGGCCTC AGAGCCTCAC
    CTGACTCCCC AGCTGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836490.1
    CDS476..4276
    Translation

    Target ORF information:

    RefSeq Version XM_024980722.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980722.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AGATCTCAGA CGGGCAGTTA CTCCAGTCTC TCCAGGCCTC AGAGCCTCAC
    CTGACTCCCC AGCTGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226754
    Clone ID Related Accession (Same CDS sequence) XM_024980725.1 , XM_024980724.1 , XM_024980723.1
    Accession Version XM_024980725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836493.1
    CDS545..4048
    Translation

    Target ORF information:

    RefSeq Version XM_024980725.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226754
    Clone ID Related Accession (Same CDS sequence) XM_024980725.1 , XM_024980724.1 , XM_024980723.1
    Accession Version XM_024980724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836492.1
    CDS710..4213
    Translation

    Target ORF information:

    RefSeq Version XM_024980724.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226754
    Clone ID Related Accession (Same CDS sequence) XM_024980725.1 , XM_024980724.1 , XM_024980723.1
    Accession Version XM_024980723.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836491.1
    CDS746..4249
    Translation

    Target ORF information:

    RefSeq Version XM_024980723.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980723.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
    AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT
    GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA
    ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC
    AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG
    ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG
    ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT
    GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG
    ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA
    GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG
    GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA
    AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC
    CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG
    GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA
    GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG
    GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT
    GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG
    AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA
    GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA
    GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT
    TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG
    AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT
    CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG
    GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT
    TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT
    CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC
    TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG
    AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT
    GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG
    GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC
    ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG
    CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC
    TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC
    GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC
    CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG
    AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC
    GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA
    TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC
    CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA
    GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC
    ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG
    ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC
    AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC
    GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA
    GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT
    TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA
    ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT
    GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA
    GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA
    CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG
    GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT
    CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG
    TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC
    CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT
    CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA
    CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG
    ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC
    CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT
    GAAGAGACCA GGCCGGGCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226755
    Clone ID Related Accession (Same CDS sequence) XM_024980726.1
    Accession Version XM_024980726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG ATCCAGGTCC TGGTAAGGAG
    CATTCACTTA CTCAAGTTTT GGTCACATGC CTGCATCATA GCCACCAGGG AATAACAGCT
    TCCAGGGTGG GAGGCCCAGC TTGTCTCCTG CTGAGACCCC CCACACTGGT GGGGAAGGGG
    TTCTTCCCTA AAAGAAGACT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836494.1
    CDS480..3983
    Translation

    Target ORF information:

    RefSeq Version XM_024980726.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG ATCCAGGTCC TGGTAAGGAG
    CATTCACTTA CTCAAGTTTT GGTCACATGC CTGCATCATA GCCACCAGGG AATAACAGCT
    TCCAGGGTGG GAGGCCCAGC TTGTCTCCTG CTGAGACCCC CCACACTGGT GGGGAAGGGG
    TTCTTCCCTA AAAGAAGACT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51512
    Clone ID Related Accession (Same CDS sequence) XM_024980719.1
    Accession Version XM_024980719.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG
    ATGAGTGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA
    GCAGCACAGG TGAGGCTCTT CTGTCCGTCT TCTCCTGCCC CTCCGGAGGA CGAGCACATG
    GAGTCTCTGG GGACCGAGGA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCGAGGCG
    GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCA TGGCCGGCTT CTACCACTGG
    CCCAACACAG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT
    GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCGCA GCTGGCTGGC TGCAGGGCCT
    CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG
    GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCCGTCCT GGAAAACCCC
    AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC
    ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC
    TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGATGTTC
    CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC
    ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG
    AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC
    AGGTTGCAAA TAAGAAGCAA GAAAGATGGG ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA
    GGAGATCTCA GACGGGCAGT TACTCCAGTC TCTCCAGGCC TCAGAGCCTC ACCTGACTCC
    CCAGCTGCCC TGAGATTGAG GCACTTTGTG GACCGACATC GGGAGCAGCT GGTGGCTCGA
    GTGACATCCG TGGACCCTAT CCTGGACAAG TTGCATGGAC AGGTGCTGAG CGAAGAACAG
    TATGAGGGGG TGCGGGCAGA GACCACTGCA CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC
    AGCCGATCCT GGGACTGGGC CTGCAAAGAT CGCCTCTACC AAGCCCTGAA GGAGACCCAT
    CCTCACCTAA TCATGGAACT CTGGGAGAAG TGGGGCAGTG AAGAGACCAG GCCGGGCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836487.1
    CDS477..4619
    Translation

    Target ORF information:

    RefSeq Version XM_024980719.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG
    ATGAGTGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA
    GCAGCACAGG TGAGGCTCTT CTGTCCGTCT TCTCCTGCCC CTCCGGAGGA CGAGCACATG
    GAGTCTCTGG GGACCGAGGA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCGAGGCG
    GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCA TGGCCGGCTT CTACCACTGG
    CCCAACACAG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT
    GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCGCA GCTGGCTGGC TGCAGGGCCT
    CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG
    GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG
    GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCCGTCCT GGAAAACCCC
    AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC
    ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC
    TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGATGTTC
    CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC
    ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG
    AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC
    AGGTTGCAAA TAAGAAGCAA GAAAGATGGG ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA
    GGAGATCTCA GACGGGCAGT TACTCCAGTC TCTCCAGGCC TCAGAGCCTC ACCTGACTCC
    CCAGCTGCCC TGAGATTGAG GCACTTTGTG GACCGACATC GGGAGCAGCT GGTGGCTCGA
    GTGACATCCG TGGACCCTAT CCTGGACAAG TTGCATGGAC AGGTGCTGAG CGAAGAACAG
    TATGAGGGGG TGCGGGCAGA GACCACTGCA CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC
    AGCCGATCCT GGGACTGGGC CTGCAAAGAT CGCCTCTACC AAGCCCTGAA GGAGACCCAT
    CCTCACCTAA TCATGGAACT CTGGGAGAAG TGGGGCAGTG AAGAGACCAG GCCGGGCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10079
    Clone ID Related Accession (Same CDS sequence) XM_003587406.3 , XM_003583564.3 , XM_003587406.4
    Accession Version XM_003587406.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003587406.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587454.2
    CDS472..4704
    Translation

    Target ORF information:

    RefSeq Version XM_003587406.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587406.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10079
    Clone ID Related Accession (Same CDS sequence) XM_003587406.3 , XM_003583564.3 , XM_003587406.4
    Accession Version XM_003583564.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003583564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003583612.2
    CDS472..4704
    Translation

    Target ORF information:

    RefSeq Version XM_003583564.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003583564.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10079
    Clone ID Related Accession (Same CDS sequence) XM_003587406.3 , XM_003583564.3 , XM_003587406.4
    Accession Version XM_003587406.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587406.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587454.2
    CDS476..4708
    Translation

    Target ORF information:

    RefSeq Version XM_003587406.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587406.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC
    CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG
    AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG
    ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC
    CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC
    CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG
    AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA
    GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA
    GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC
    CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG
    TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC
    GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA
    CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG
    TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG
    CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT
    TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC
    ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG
    ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG
    GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC
    CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT
    GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT
    GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC
    ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC
    TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA
    AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA
    TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG
    TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG
    CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA
    CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC
    GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC
    AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC
    ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA
    CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG
    AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG
    AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG
    AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA
    ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG
    GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT
    GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG
    CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG
    GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG
    ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG
    AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT
    TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG
    GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT
    CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA
    GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC
    CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT
    GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC
    CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG
    ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG
    ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC
    CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG
    AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG
    CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA
    CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG
    TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG
    ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC
    TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG
    GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG
    TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA
    CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT
    CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG
    TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51513
    Clone ID Related Accession (Same CDS sequence) XM_010824036.1 , XM_010815958.1 , XM_010815958.2
    Accession Version XM_010824036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822338.1
    CDS472..4539
    Translation

    Target ORF information:

    RefSeq Version XM_010824036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51513
    Clone ID Related Accession (Same CDS sequence) XM_010824036.1 , XM_010815958.1 , XM_010815958.2
    Accession Version XM_010815958.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814260.1
    CDS472..4539
    Translation

    Target ORF information:

    RefSeq Version XM_010815958.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51513
    Clone ID Related Accession (Same CDS sequence) XM_010824036.1 , XM_010815958.1 , XM_010815958.2
    Accession Version XM_010815958.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814260.1
    CDS478..4545
    Translation

    Target ORF information:

    RefSeq Version XM_010815958.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815958.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA
    GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA
    TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG
    AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA
    AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA
    AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA
    GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT
    TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA
    GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA
    GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC
    ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC
    GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC
    CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT
    CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT
    TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT
    TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA
    AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC
    CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC
    CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG
    GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG
    GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT
    CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC
    TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC
    ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA
    ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT
    TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG
    GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC
    GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC
    AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT
    CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG
    AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT
    GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT
    CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC
    AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG
    ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG
    GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG
    TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA
    AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG
    CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG
    ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG
    AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC
    TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG
    CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC
    GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG
    AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC
    CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC
    CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG
    GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC
    GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG
    AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG
    GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG
    TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC
    GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT
    CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA
    GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT
    CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA
    AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG
    GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA
    TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC
    CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG
    GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC
    TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG
    GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51514
    Clone ID Related Accession (Same CDS sequence) XM_010815959.1 , XM_010824037.1 , XM_010815959.2
    Accession Version XM_010815959.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814261.1
    CDS472..4503
    Translation

    Target ORF information:

    RefSeq Version XM_010815959.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51514
    Clone ID Related Accession (Same CDS sequence) XM_010815959.1 , XM_010824037.1 , XM_010815959.2
    Accession Version XM_010824037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822339.1
    CDS472..4503
    Translation

    Target ORF information:

    RefSeq Version XM_010824037.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51514
    Clone ID Related Accession (Same CDS sequence) XM_010815959.1 , XM_010824037.1 , XM_010815959.2
    Accession Version XM_010815959.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814261.1
    CDS477..4508
    Translation

    Target ORF information:

    RefSeq Version XM_010815959.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815959.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
    GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT
    GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG
    GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA
    TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC
    TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA
    TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT
    TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA
    CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA
    CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC
    GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG
    GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC
    ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC
    CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG
    GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG
    CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG
    AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC
    AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA
    GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC
    TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT
    AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG
    GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA
    CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT
    CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA
    GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT
    GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC
    ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG
    GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG
    ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA
    GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG
    TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT
    CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA
    GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA
    TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC
    AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT
    CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC
    TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA
    GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT
    GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC
    GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG
    TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG
    GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG
    AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC
    CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT
    CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT
    GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC
    TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC
    GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT
    ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC
    TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT
    GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT
    GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT
    CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC
    AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG
    GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC
    TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC
    TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA
    AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC
    TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC
    TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG
    TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG
    GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA
    CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG
    GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC
    GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG
    TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG
    GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG
    CCGGGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.