NLRP1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010815959.1	XP_010814261.1	NACHT, LRR and PYD domains-containing protein 1 isoform X3
XM_024980725.1	XP_024836493.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_024980719.1	XP_024836487.1	NACHT, LRR and PYD domains-containing protein 1 isoform X2
XM_024980724.1	XP_024836492.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_024980726.1	XP_024836494.1	NACHT, LRR and PYD domains-containing protein 1 isoform X9
XM_003587406.3	XP_003587454.2	NACHT, LRR and PYD domains-containing protein 1 isoform X1
XM_010824036.1	XP_010822338.1	NACHT, LRR and PYD domains-containing protein 1 isoform X2
XM_010824037.1	XP_010822339.1	NACHT, LRR and PYD domains-containing protein 1 isoform X3
XM_024980720.1	XP_024836488.1	NACHT, LRR and PYD domains-containing protein 1 isoform X4
XM_024980721.1	XP_024836489.1	NACHT, LRR and PYD domains-containing protein 1 isoform X6
XM_024980723.1	XP_024836491.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_010815958.1	XP_010814260.1	NACHT, LRR and PYD domains-containing protein 1 isoform X2
XM_003583564.3	XP_003583612.2	NACHT, LRR and PYD domains-containing protein 1 isoform X1
XM_003587406.4	XP_003587454.2	NACHT, LRR and PYD domains-containing protein 1 isoform X1
XM_010815958.2	XP_010814260.1	NACHT, LRR and PYD domains-containing protein 1 isoform X3
XM_010815959.2	XP_010814261.1	NACHT, LRR and PYD domains-containing protein 1 isoform X5
XM_024980722.1	XP_024836490.1	NACHT, LRR and PYD domains-containing protein 1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226751	XM_024980720.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226752	XM_024980721.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226753	XM_024980722.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226754	XM_024980725.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226754	XM_024980724.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226754	XM_024980723.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226755	XM_024980726.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa51512	XM_024980719.1 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa10079	XM_003587406.4 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa10079	XM_003583564.3 Latest version!	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51513	XM_010815958.2 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51513	XM_010824036.1 Latest version!	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51514	XM_010824037.1 Latest version!	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51514	XM_010815959.2 Latest version!	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa10079	XM_003587406.3	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51513	XM_010815958.1	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa51514	XM_010815959.1	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

NLRP1 ( XM_024980720.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836488.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa226751
Clone ID Related Accession (Same CDS sequence)	XM_024980720.1
Accession Version	XM_024980720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGCTGGCAG GCTGCGGCCT CACGTCTGGC 
TGCTGCCAGG ACCTGGCCTC CATGCTCAGG GTCAGCCCCA GCCTGATGGA GCTAAACCTG 
CTGCAGAATG ACCTGGACGA CCTCGGCGTG AGGCTGCTCT GTGAAGGACT CAGGCACCCT 
TCCTGCCAGC TCATATGCCT ATGGCTGGAC CAGACTCAGC TGAGCGAGGA GGTGACCAAG 
ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA 
AGTCTGAGGA TCCTTCACGA GGGCCCAGGT GGAGCAGAGA TGAGTGATAC ATCCTCCCTC 
AAGCGGCAGA GACAAGCATC AGAGGGCAGC TCTCCTCAAG CAGCACAGGT GAGGCTCTTC 
TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC GAGCACATGG AGTCTCTGGG GACCGAGGAT 
GACCTCTGGG GCCCCACAGG GCCTGTGGCT ACCGAGGCGG TTGACGAAGA GAGGAGCCTG 
TACCGCGTTC ACTTGCCCAT GGCCGGCTTC TACCACTGGC CCAACACAGG TCTCCGCTTT 
GAGGTGAGAG GGCCAGTGAC CGTTGAAATT GAATTCTGTG CCTGGGACCA GTTCCTGAAC 
AGGAATTTCC CACAGCGCAG CTGGCTGGCT GCAGGGCCTC TGTTTGACAT CAAGGCTGAA 
CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT CACTTTGTGG CCTTCCGAGG GAACGATGTG 
GACATCTCCC AGTTTCAAGT GGCCCACTTC AAAGAGGAGG GGATGCTCCT GGAGAAGCCA 
GCCAGGGTGA CGCCATGTTA CGCCGTCCTG GAAAACCCCA GCTTCTCGCC CATGGGAGTT 
CTACTGAGGA TGGTCCAAAC TGCCCTGCGC TTCATTCCCA TCACCTCCAC TGTGTTGCTC 
TACCATTACC TCCAGCCTGA AGAAGTTACC TTCCACCTCT ACCTGATCCC CAGCGATTGC 
TCCATTCAGA AGGCCATAGA CAATGAAGAA AAGATGTTCC AGTTTGTGCG AATTCACAAG 
CCACCCCCGC TGACCCCACT CTACATGGGC TCCCGCTACA TTGTGTCTGG TTCAGAGAAG 
CTGGAAATAC TGCCCGAGGA ATTGGAGCTC TGCTATCGGA GCCCCAGAGA ATCTCAGCTG 
TTCTCTGAGT TCTACGTTCG CCATTTGAGG TCAGGGATCA GGTTGCAAAT AAGAAGCAAG 
AAAGATGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT 
ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA CCTGACTCCC CAGCTGCCCT GAGATTGAGG 
CACTTTGTGG ACCGACATCG GGAGCAGCTG GTGGCTCGAG TGACATCCGT GGACCCTATC 
CTGGACAAGT TGCATGGACA GGTGCTGAGC GAAGAACAGT ATGAGGGGGT GCGGGCAGAG 
ACCACTGCAC CAGGCCAGAT GCGGAGGCTG TTTGGTTTCA GCCGATCCTG GGACTGGGCC 
TGCAAAGATC GCCTCTACCA AGCCCTGAAG GAGACCCATC CTCACCTAAT CATGGAACTC 
TGGGAGAAGT GGGGCAGTGA AGAGACCAGG CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836488.1
CDS	477..4538
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980720.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980720.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGCTGGCAG GCTGCGGCCT CACGTCTGGC 
TGCTGCCAGG ACCTGGCCTC CATGCTCAGG GTCAGCCCCA GCCTGATGGA GCTAAACCTG 
CTGCAGAATG ACCTGGACGA CCTCGGCGTG AGGCTGCTCT GTGAAGGACT CAGGCACCCT 
TCCTGCCAGC TCATATGCCT ATGGCTGGAC CAGACTCAGC TGAGCGAGGA GGTGACCAAG 
ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA 
AGTCTGAGGA TCCTTCACGA GGGCCCAGGT GGAGCAGAGA TGAGTGATAC ATCCTCCCTC 
AAGCGGCAGA GACAAGCATC AGAGGGCAGC TCTCCTCAAG CAGCACAGGT GAGGCTCTTC 
TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC GAGCACATGG AGTCTCTGGG GACCGAGGAT 
GACCTCTGGG GCCCCACAGG GCCTGTGGCT ACCGAGGCGG TTGACGAAGA GAGGAGCCTG 
TACCGCGTTC ACTTGCCCAT GGCCGGCTTC TACCACTGGC CCAACACAGG TCTCCGCTTT 
GAGGTGAGAG GGCCAGTGAC CGTTGAAATT GAATTCTGTG CCTGGGACCA GTTCCTGAAC 
AGGAATTTCC CACAGCGCAG CTGGCTGGCT GCAGGGCCTC TGTTTGACAT CAAGGCTGAA 
CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT CACTTTGTGG CCTTCCGAGG GAACGATGTG 
GACATCTCCC AGTTTCAAGT GGCCCACTTC AAAGAGGAGG GGATGCTCCT GGAGAAGCCA 
GCCAGGGTGA CGCCATGTTA CGCCGTCCTG GAAAACCCCA GCTTCTCGCC CATGGGAGTT 
CTACTGAGGA TGGTCCAAAC TGCCCTGCGC TTCATTCCCA TCACCTCCAC TGTGTTGCTC 
TACCATTACC TCCAGCCTGA AGAAGTTACC TTCCACCTCT ACCTGATCCC CAGCGATTGC 
TCCATTCAGA AGGCCATAGA CAATGAAGAA AAGATGTTCC AGTTTGTGCG AATTCACAAG 
CCACCCCCGC TGACCCCACT CTACATGGGC TCCCGCTACA TTGTGTCTGG TTCAGAGAAG 
CTGGAAATAC TGCCCGAGGA ATTGGAGCTC TGCTATCGGA GCCCCAGAGA ATCTCAGCTG 
TTCTCTGAGT TCTACGTTCG CCATTTGAGG TCAGGGATCA GGTTGCAAAT AAGAAGCAAG 
AAAGATGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT 
ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA CCTGACTCCC CAGCTGCCCT GAGATTGAGG 
CACTTTGTGG ACCGACATCG GGAGCAGCTG GTGGCTCGAG TGACATCCGT GGACCCTATC 
CTGGACAAGT TGCATGGACA GGTGCTGAGC GAAGAACAGT ATGAGGGGGT GCGGGCAGAG 
ACCACTGCAC CAGGCCAGAT GCGGAGGCTG TTTGGTTTCA GCCGATCCTG GGACTGGGCC 
TGCAAAGATC GCCTCTACCA AGCCCTGAAG GAGACCCATC CTCACCTAAT CATGGAACTC 
TGGGAGAAGT GGGGCAGTGA AGAGACCAGG CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980721.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836489.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa226752
Clone ID Related Accession (Same CDS sequence)	XM_024980721.1
Accession Version	XM_024980721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGGAA GCAGACAGAG ATACCAGGAT 
CAAAGGAGAG AGACACCTTG CTCCACCTGG TCATGGAAAA ATGAAGATTT GCACCCAAAA 
TTCATGCAGT TGCTACTTCT ACACACTCCT TACCACAAAG ACTACGAGTC CTTGAACCGG 
GAAAGCTGGG ATCACGGGGT GGTTGAGGGG CAAGGGCATT TGATTGAGGT CAGAGACTTA 
TTTGGCTCAG ACCTGGGTTC CCAGGAAGGG CCTCACACAG TCATATTGCA TGGAGTTGCT 
GGAATTGGGA AGTCCACACT GGCCAGGCAC ATAAGAAGAG CCTGGGAGGA GGGCCAGCTA 
TACAGGAACC ACTTCCAGCA TGTCTTCTAC TTCAACTGCA GAGAGCTGGC CACGTCCAAG 
CTGACAAGTC TGGAGGAACT CATCACAAAA GACCAGTCCA CCACTGTGGC TCCCGCTGGA 
CAGATCCAGT CTCAGCCGGA GCAGCTGCTC TTCATCCTCG ATGGTTTAGA TGAACTGGAA 
TGTGTCTCAG AGGAGAAGAG GGCAGAGCTC TGTCTGCACC GGAGCCAGCA GCAGTCTGTG 
CAGACACTGC TGGGCAGTTT GGTGGAGAAA ACCATATTTC CAGAGGCATC CTTGCTGATC 
ACAGCTCGGA CTGCAGCTCT GCAGAAGTTC ATTCCTTCTT TGAAGCAGCC ATGTTGGGTG 
GAGGTCCTGG GGTTCTCCGA GTCTTCCAGG AAGGACTATT TCTATGAATA TTTCACAGAG 
GAAAGCCAAG CAACTAGAGC CTTTAGCGTG GTTGAATCAA ACCAAGCACT CTTGACCATG 
TGCCTCACGC CCTTGGTGTC CTGGCTGGCC TGCACTTGCC TGAAACAGCA GATGGAGGCA 
GGGGAAGAAC TCTCACTGAA GTCGCAGACC ATGACAGCCC TCTATCTGCG CTACCTTGCC 
CAAGCTATCC AAGCTCAGCC CCTGGGCACT CAGCTGAGGG GCTTCTGTAC TCTTGCTGCT 
GAGGGTATCT GTCAAGGAAA GACTCTGTTC AGTCCAGGGG ACCTCAAGAA GCATGGATTA 
GATGGGGTCA TCAGTGCCAC TCCCTTAAAG ATGGATGTTC TTCAAAAACA CCCCACATCT 
CTGAACTACA GCTTCATTCA CCTGTGTTTC CAGGAGTTCT TTGCAGCAGT GTCCTTTGCC 
TTAGGGGACA TTCGGGACAA AAGTGACCAT CCTAACAGCA CTGGAAGCAT GGAAAAGTTG 
GTAGAAGTAT ATGGGATCAA TAACCTCTTT GGGGCACCAA CCGTGCATTT CCTATCCGGC 
CTTTTGAGTG AGCAAGGAGC GAGAGAGATG GAGAACATTT TCCAATGCAA GCTGTCTTGG 
GAGAGAAAGC GGGAGTCACT GCTGCGGTGG GCTGAATTGG AACTCCGGCG TGAGCATTAC 
TCTCTGCAAT TGTTTCACTG TTTGTACGAG ATTCAGGACG AGGAGTTCCT GACACAAGCG 
ATGGCCCATT TCCAAGGAAT GAGAGTGTGC ATCCGAACCA GCATGGAGCT GCTCGTGTTC 
ACTTTCTGCA TTAGATTCTG CAGCCACGTG TGGCGGCTTC AACTGAATGA GGGCAGACAA 
CATCCAGGAG CATGCAGGCC CACCGACACA GTGCTGTTGA GCTGGGTCCC GGCCACAGAA 
GCCTGCTGGC AGGTTCTCTG CTCTGTTCTC CAGGTCACTG GAAACCTGAA GGAACTGGAC 
CTGAGTAGAA ACATCTTGAG CCGCTCTGCA GTACAGAGTC TTGATGAGGC CCTGAGATGC 
CCTGGGTGCC AGCTAGAGAC CCTGCGGTTG GCCAGCTGCA GCCTCACAGC TGAGAGCTGC 
AAGGACATTG CCTCTGGGCT GAGCACCAGC CAAACCCTGA TGCAATTGGA ATTGAGCTTC 
AACACACTCC TGGACGCAGG AGCTGAGCAC CTTTGCCAGG GCCTGAGACA GCCAACCTGC 
AAGCTGCAGC GACTGTTGCT GGCAGGCTGC GGCCTCACGT CTGGCTGCTG CCAGGACCTG 
GCCTCCATGC TCAGGGTCAG CCCCAGCCTG ATGGAGCTAA ACCTGCTGCA GAATGACCTG 
GACGACCTCG GCGTGAGGCT GCTCTGTGAA GGACTCAGGC ACCCTTCCTG CCAGCTCATA 
TGCCTATGGC TGGACCAGAC TCAGCTGAGC GAGGAGGTGA CCAAGATGCT GAGGGCCCTG 
GAGAGGCAGA AGCCTCAGCT GCTAGTCTCC GGGATATGGA AGCCAAGTCT GAGGATCCTT 
CACGAGGGCC CAGGTGGAGC AGAGATGAGT GATACATCCT CCCTCAAGCG GCAGAGACAA 
GCATCAGAGG GCAGCTCTCC TCAAGCAGCA CAGGTGAGGC TCTTCTGTCC GTCTTCTCCT 
GCCCCTCCGG AGGACGAGCA CATGGAGTCT CTGGGGACCG AGGATGACCT CTGGGGCCCC 
ACAGGGCCTG TGGCTACCGA GGCGGTTGAC GAAGAGAGGA GCCTGTACCG CGTTCACTTG 
CCCATGGCCG GCTTCTACCA CTGGCCCAAC ACAGGTCTCC GCTTTGAGGT GAGAGGGCCA 
GTGACCGTTG AAATTGAATT CTGTGCCTGG GACCAGTTCC TGAACAGGAA TTTCCCACAG 
CGCAGCTGGC TGGCTGCAGG GCCTCTGTTT GACATCAAGG CTGAACCAGG CGCTGTGGCA 
GCCGTGTTCC TCCCTCACTT TGTGGCCTTC CGAGGGAACG ATGTGGACAT CTCCCAGTTT 
CAAGTGGCCC ACTTCAAAGA GGAGGGGATG CTCCTGGAGA AGCCAGCCAG GGTGACGCCA 
TGTTACGCCG TCCTGGAAAA CCCCAGCTTC TCGCCCATGG GAGTTCTACT GAGGATGGTC 
CAAACTGCCC TGCGCTTCAT TCCCATCACC TCCACTGTGT TGCTCTACCA TTACCTCCAG 
CCTGAAGAAG TTACCTTCCA CCTCTACCTG ATCCCCAGCG ATTGCTCCAT TCAGAAGGCC 
ATAGACAATG AAGAAAAGAT GTTCCAGTTT GTGCGAATTC ACAAGCCACC CCCGCTGACC 
CCACTCTACA TGGGCTCCCG CTACATTGTG TCTGGTTCAG AGAAGCTGGA AATACTGCCC 
GAGGAATTGG AGCTCTGCTA TCGGAGCCCC AGAGAATCTC AGCTGTTCTC TGAGTTCTAC 
GTTCGCCATT TGAGGTCAGG GATCAGGTTG CAAATAAGAA GCAAGAAAGA TGGGACTGTG 
GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC AGTCTCTCCA 
GGCCTCAGAG CCTCACCTGA CTCCCCAGCT GCCCTGAGAT TGAGGCACTT TGTGGACCGA 
CATCGGGAGC AGCTGGTGGC TCGAGTGACA TCCGTGGACC CTATCCTGGA CAAGTTGCAT 
GGACAGGTGC TGAGCGAAGA ACAGTATGAG GGGGTGCGGG CAGAGACCAC TGCACCAGGC 
CAGATGCGGA GGCTGTTTGG TTTCAGCCGA TCCTGGGACT GGGCCTGCAA AGATCGCCTC 
TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTAATCATGG AACTCTGGGA GAAGTGGGGC 
AGTGAAGAGA CCAGGCCGGG CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836489.1
CDS	478..4344
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980721.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980721.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGGAA GCAGACAGAG ATACCAGGAT 
CAAAGGAGAG AGACACCTTG CTCCACCTGG TCATGGAAAA ATGAAGATTT GCACCCAAAA 
TTCATGCAGT TGCTACTTCT ACACACTCCT TACCACAAAG ACTACGAGTC CTTGAACCGG 
GAAAGCTGGG ATCACGGGGT GGTTGAGGGG CAAGGGCATT TGATTGAGGT CAGAGACTTA 
TTTGGCTCAG ACCTGGGTTC CCAGGAAGGG CCTCACACAG TCATATTGCA TGGAGTTGCT 
GGAATTGGGA AGTCCACACT GGCCAGGCAC ATAAGAAGAG CCTGGGAGGA GGGCCAGCTA 
TACAGGAACC ACTTCCAGCA TGTCTTCTAC TTCAACTGCA GAGAGCTGGC CACGTCCAAG 
CTGACAAGTC TGGAGGAACT CATCACAAAA GACCAGTCCA CCACTGTGGC TCCCGCTGGA 
CAGATCCAGT CTCAGCCGGA GCAGCTGCTC TTCATCCTCG ATGGTTTAGA TGAACTGGAA 
TGTGTCTCAG AGGAGAAGAG GGCAGAGCTC TGTCTGCACC GGAGCCAGCA GCAGTCTGTG 
CAGACACTGC TGGGCAGTTT GGTGGAGAAA ACCATATTTC CAGAGGCATC CTTGCTGATC 
ACAGCTCGGA CTGCAGCTCT GCAGAAGTTC ATTCCTTCTT TGAAGCAGCC ATGTTGGGTG 
GAGGTCCTGG GGTTCTCCGA GTCTTCCAGG AAGGACTATT TCTATGAATA TTTCACAGAG 
GAAAGCCAAG CAACTAGAGC CTTTAGCGTG GTTGAATCAA ACCAAGCACT CTTGACCATG 
TGCCTCACGC CCTTGGTGTC CTGGCTGGCC TGCACTTGCC TGAAACAGCA GATGGAGGCA 
GGGGAAGAAC TCTCACTGAA GTCGCAGACC ATGACAGCCC TCTATCTGCG CTACCTTGCC 
CAAGCTATCC AAGCTCAGCC CCTGGGCACT CAGCTGAGGG GCTTCTGTAC TCTTGCTGCT 
GAGGGTATCT GTCAAGGAAA GACTCTGTTC AGTCCAGGGG ACCTCAAGAA GCATGGATTA 
GATGGGGTCA TCAGTGCCAC TCCCTTAAAG ATGGATGTTC TTCAAAAACA CCCCACATCT 
CTGAACTACA GCTTCATTCA CCTGTGTTTC CAGGAGTTCT TTGCAGCAGT GTCCTTTGCC 
TTAGGGGACA TTCGGGACAA AAGTGACCAT CCTAACAGCA CTGGAAGCAT GGAAAAGTTG 
GTAGAAGTAT ATGGGATCAA TAACCTCTTT GGGGCACCAA CCGTGCATTT CCTATCCGGC 
CTTTTGAGTG AGCAAGGAGC GAGAGAGATG GAGAACATTT TCCAATGCAA GCTGTCTTGG 
GAGAGAAAGC GGGAGTCACT GCTGCGGTGG GCTGAATTGG AACTCCGGCG TGAGCATTAC 
TCTCTGCAAT TGTTTCACTG TTTGTACGAG ATTCAGGACG AGGAGTTCCT GACACAAGCG 
ATGGCCCATT TCCAAGGAAT GAGAGTGTGC ATCCGAACCA GCATGGAGCT GCTCGTGTTC 
ACTTTCTGCA TTAGATTCTG CAGCCACGTG TGGCGGCTTC AACTGAATGA GGGCAGACAA 
CATCCAGGAG CATGCAGGCC CACCGACACA GTGCTGTTGA GCTGGGTCCC GGCCACAGAA 
GCCTGCTGGC AGGTTCTCTG CTCTGTTCTC CAGGTCACTG GAAACCTGAA GGAACTGGAC 
CTGAGTAGAA ACATCTTGAG CCGCTCTGCA GTACAGAGTC TTGATGAGGC CCTGAGATGC 
CCTGGGTGCC AGCTAGAGAC CCTGCGGTTG GCCAGCTGCA GCCTCACAGC TGAGAGCTGC 
AAGGACATTG CCTCTGGGCT GAGCACCAGC CAAACCCTGA TGCAATTGGA ATTGAGCTTC 
AACACACTCC TGGACGCAGG AGCTGAGCAC CTTTGCCAGG GCCTGAGACA GCCAACCTGC 
AAGCTGCAGC GACTGTTGCT GGCAGGCTGC GGCCTCACGT CTGGCTGCTG CCAGGACCTG 
GCCTCCATGC TCAGGGTCAG CCCCAGCCTG ATGGAGCTAA ACCTGCTGCA GAATGACCTG 
GACGACCTCG GCGTGAGGCT GCTCTGTGAA GGACTCAGGC ACCCTTCCTG CCAGCTCATA 
TGCCTATGGC TGGACCAGAC TCAGCTGAGC GAGGAGGTGA CCAAGATGCT GAGGGCCCTG 
GAGAGGCAGA AGCCTCAGCT GCTAGTCTCC GGGATATGGA AGCCAAGTCT GAGGATCCTT 
CACGAGGGCC CAGGTGGAGC AGAGATGAGT GATACATCCT CCCTCAAGCG GCAGAGACAA 
GCATCAGAGG GCAGCTCTCC TCAAGCAGCA CAGGTGAGGC TCTTCTGTCC GTCTTCTCCT 
GCCCCTCCGG AGGACGAGCA CATGGAGTCT CTGGGGACCG AGGATGACCT CTGGGGCCCC 
ACAGGGCCTG TGGCTACCGA GGCGGTTGAC GAAGAGAGGA GCCTGTACCG CGTTCACTTG 
CCCATGGCCG GCTTCTACCA CTGGCCCAAC ACAGGTCTCC GCTTTGAGGT GAGAGGGCCA 
GTGACCGTTG AAATTGAATT CTGTGCCTGG GACCAGTTCC TGAACAGGAA TTTCCCACAG 
CGCAGCTGGC TGGCTGCAGG GCCTCTGTTT GACATCAAGG CTGAACCAGG CGCTGTGGCA 
GCCGTGTTCC TCCCTCACTT TGTGGCCTTC CGAGGGAACG ATGTGGACAT CTCCCAGTTT 
CAAGTGGCCC ACTTCAAAGA GGAGGGGATG CTCCTGGAGA AGCCAGCCAG GGTGACGCCA 
TGTTACGCCG TCCTGGAAAA CCCCAGCTTC TCGCCCATGG GAGTTCTACT GAGGATGGTC 
CAAACTGCCC TGCGCTTCAT TCCCATCACC TCCACTGTGT TGCTCTACCA TTACCTCCAG 
CCTGAAGAAG TTACCTTCCA CCTCTACCTG ATCCCCAGCG ATTGCTCCAT TCAGAAGGCC 
ATAGACAATG AAGAAAAGAT GTTCCAGTTT GTGCGAATTC ACAAGCCACC CCCGCTGACC 
CCACTCTACA TGGGCTCCCG CTACATTGTG TCTGGTTCAG AGAAGCTGGA AATACTGCCC 
GAGGAATTGG AGCTCTGCTA TCGGAGCCCC AGAGAATCTC AGCTGTTCTC TGAGTTCTAC 
GTTCGCCATT TGAGGTCAGG GATCAGGTTG CAAATAAGAA GCAAGAAAGA TGGGACTGTG 
GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC AGTCTCTCCA 
GGCCTCAGAG CCTCACCTGA CTCCCCAGCT GCCCTGAGAT TGAGGCACTT TGTGGACCGA 
CATCGGGAGC AGCTGGTGGC TCGAGTGACA TCCGTGGACC CTATCCTGGA CAAGTTGCAT 
GGACAGGTGC TGAGCGAAGA ACAGTATGAG GGGGTGCGGG CAGAGACCAC TGCACCAGGC 
CAGATGCGGA GGCTGTTTGG TTTCAGCCGA TCCTGGGACT GGGCCTGCAA AGATCGCCTC 
TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTAATCATGG AACTCTGGGA GAAGTGGGGC 
AGTGAAGAGA CCAGGCCGGG CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980722.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836490.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.

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CloneID	OBa226753
Clone ID Related Accession (Same CDS sequence)	XM_024980722.1
Accession Version	XM_024980722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AGATCTCAGA CGGGCAGTTA CTCCAGTCTC TCCAGGCCTC AGAGCCTCAC 
CTGACTCCCC AGCTGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836490.1
CDS	476..4276
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEEISDGQLLQSLQASEPHLTPQLP

Target ORF information:

RefSeq Version	XM_024980722.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980722.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AGATCTCAGA CGGGCAGTTA CTCCAGTCTC TCCAGGCCTC AGAGCCTCAC 
CTGACTCCCC AGCTGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980725.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836493.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.

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CloneID	OBa226754
Clone ID Related Accession (Same CDS sequence)	XM_024980725.1 , XM_024980724.1 , XM_024980723.1
Accession Version	XM_024980725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836493.1
CDS	545..4048
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980725.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980725.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980724.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836492.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa226754
Clone ID Related Accession (Same CDS sequence)	XM_024980725.1 , XM_024980724.1 , XM_024980723.1
Accession Version	XM_024980724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836492.1
CDS	710..4213
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980724.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980724.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980723.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836491.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X8, mRNA.

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CloneID	OBa226754
Clone ID Related Accession (Same CDS sequence)	XM_024980725.1 , XM_024980724.1 , XM_024980723.1
Accession Version	XM_024980723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836491.1
CDS	746..4249
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980723.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980723.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ACGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTTCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAC GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGACAG 
ATCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGT 
GTCTCAGAGG AGAAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GTCTGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTGGGGT TCTCCGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAGCGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGC 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AACAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAAGTC GCAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGCACTCAG CTGAGGGGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGTC AAGGAAAGAC TCTGTTCAGT CCAGGGGACC TCAAGAAGCA TGGATTAGAT 
GGGGTCATCA GTGCCACTCC CTTAAAGATG GATGTTCTTC AAAAACACCC CACATCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATTC GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTCTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAG AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG AGTCACTGCT GCGGTGGGCT GAATTGGAAC TCCGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAATGAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGTGG CGGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCAT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAAGCC 
TGCTGGCAGG TTCTCTGCTC TGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTAGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGCCAGC TAGAGACCCT GCGGTTGGCC AGCTGCAGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CACCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
GACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGACCA AGATGCTGAG GGCCCTGGAG 
AGGCAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGTGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGGCTCT TCTGTCCGTC TTCTCCTGCC 
CCTCCGGAGG ACGAGCACAT GGAGTCTCTG GGGACCGAGG ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCGAGGC GGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
ATGGCCGGCT TCTACCACTG GCCCAACACA GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCGC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCCGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGATGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT CTCTCCAGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGACAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAGGC TGTTTGGTTT CAGCCGATCC TGGGACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980726.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836494.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa226755
Clone ID Related Accession (Same CDS sequence)	XM_024980726.1
Accession Version	XM_024980726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG ATCCAGGTCC TGGTAAGGAG 
CATTCACTTA CTCAAGTTTT GGTCACATGC CTGCATCATA GCCACCAGGG AATAACAGCT 
TCCAGGGTGG GAGGCCCAGC TTGTCTCCTG CTGAGACCCC CCACACTGGT GGGGAAGGGG 
TTCTTCCCTA AAAGAAGACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836494.1
CDS	480..3983
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRDPGPGKEHSLTQVLVTCLHHSHQGITASRVGGPACLLLRPPTLVGKGFFPKRRL

Target ORF information:

RefSeq Version	XM_024980726.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980726.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG ATCCAGGTCC TGGTAAGGAG 
CATTCACTTA CTCAAGTTTT GGTCACATGC CTGCATCATA GCCACCAGGG AATAACAGCT 
TCCAGGGTGG GAGGCCCAGC TTGTCTCCTG CTGAGACCCC CCACACTGGT GGGGAAGGGG 
TTCTTCCCTA AAAGAAGACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_024980719.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836487.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa51512
Clone ID Related Accession (Same CDS sequence)	XM_024980719.1
Accession Version	XM_024980719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG 
ATGAGTGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA 
GCAGCACAGG TGAGGCTCTT CTGTCCGTCT TCTCCTGCCC CTCCGGAGGA CGAGCACATG 
GAGTCTCTGG GGACCGAGGA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCGAGGCG 
GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCA TGGCCGGCTT CTACCACTGG 
CCCAACACAG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT 
GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCGCA GCTGGCTGGC TGCAGGGCCT 
CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG 
GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG 
GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCCGTCCT GGAAAACCCC 
AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC 
ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC 
TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGATGTTC 
CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC 
ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG 
AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC 
AGGTTGCAAA TAAGAAGCAA GAAAGATGGG ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA 
GGAGATCTCA GACGGGCAGT TACTCCAGTC TCTCCAGGCC TCAGAGCCTC ACCTGACTCC 
CCAGCTGCCC TGAGATTGAG GCACTTTGTG GACCGACATC GGGAGCAGCT GGTGGCTCGA 
GTGACATCCG TGGACCCTAT CCTGGACAAG TTGCATGGAC AGGTGCTGAG CGAAGAACAG 
TATGAGGGGG TGCGGGCAGA GACCACTGCA CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC 
AGCCGATCCT GGGACTGGGC CTGCAAAGAT CGCCTCTACC AAGCCCTGAA GGAGACCCAT 
CCTCACCTAA TCATGGAACT CTGGGAGAAG TGGGGCAGTG AAGAGACCAG GCCGGGCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836487.1
CDS	477..4619
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_024980719.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980719.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG 
ATGAGTGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA 
GCAGCACAGG TGAGGCTCTT CTGTCCGTCT TCTCCTGCCC CTCCGGAGGA CGAGCACATG 
GAGTCTCTGG GGACCGAGGA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCGAGGCG 
GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCA TGGCCGGCTT CTACCACTGG 
CCCAACACAG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT 
GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCGCA GCTGGCTGGC TGCAGGGCCT 
CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG 
GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG 
GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCCGTCCT GGAAAACCCC 
AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC 
ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC 
TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGATGTTC 
CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC 
ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG 
AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC 
AGGTTGCAAA TAAGAAGCAA GAAAGATGGG ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA 
GGAGATCTCA GACGGGCAGT TACTCCAGTC TCTCCAGGCC TCAGAGCCTC ACCTGACTCC 
CCAGCTGCCC TGAGATTGAG GCACTTTGTG GACCGACATC GGGAGCAGCT GGTGGCTCGA 
GTGACATCCG TGGACCCTAT CCTGGACAAG TTGCATGGAC AGGTGCTGAG CGAAGAACAG 
TATGAGGGGG TGCGGGCAGA GACCACTGCA CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC 
AGCCGATCCT GGGACTGGGC CTGCAAAGAT CGCCTCTACC AAGCCCTGAA GGAGACCCAT 
CCTCACCTAA TCATGGAACT CTGGGAGAAG TGGGGCAGTG AAGAGACCAG GCCGGGCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_003587406.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587454.2 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

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CloneID	OBa10079
Clone ID Related Accession (Same CDS sequence)	XM_003587406.3 , XM_003583564.3 , XM_003587406.4
Accession Version	XM_003587406.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003587406.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587454.2
CDS	472..4704
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_003587406.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587406.3

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_003583564.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583612.2 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10079
Clone ID Related Accession (Same CDS sequence)	XM_003587406.3 , XM_003583564.3 , XM_003587406.4
Accession Version	XM_003583564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_003583564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583612.2
CDS	472..4704
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_003583564.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583564.3

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_003587406.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587454.2 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10079
Clone ID Related Accession (Same CDS sequence)	XM_003587406.3 , XM_003583564.3 , XM_003587406.4
Accession Version	XM_003587406.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587406.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587454.2
CDS	476..4708
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_003587406.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587406.4

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGACCTTTCC CCAAGCATTT ACCAAGGTTT TCCAAGTTCC 
CCATATCATG AGTCACCAAG CCAAGAGTTA CCCAATGACC CCACATCCAC CGCAGTCCTG 
AGAGCCTGTG AAATCCCACG TCATTCCTGT CCAGAGCCCA GAGAGAAGGA GGGTCCTGGG 
ACTGTATGGC CACTGGTTGA AACATCTGGA AACAGCTGCA CAGGAAGCAG ACAGAGATAC 
CAGGATCAAA GGAGAGAGAC ACCTTGCTCC ACCTGGTCAT GGAAAAATGA AGATTTGCAC 
CCAAAATTCA TGCAGTTGCT ACTTCTACAC ACTCCTTACC ACAAAGACTA CGAGTCCTTG 
AACCGGGAAA GCTGGGATCA CGGGGTGGTT GAGGGGCAAG GGCATTTGAT TGAGGTCAGA 
GACTTATTTG GCTCAGACCT GGGTTCCCAG GAAGGGCCTC ACACAGTCAT ATTGCATGGA 
GTTGCTGGAA TTGGGAAGTC CACACTGGCC AGGCACATAA GAAGAGCCTG GGAGGAGGGC 
CAGCTATACA GGAACCACTT CCAGCATGTC TTCTACTTCA ACTGCAGAGA GCTGGCCACG 
TCCAAGCTGA CAAGTCTGGA GGAACTCATC ACAAAAGACC AGTCCACCAC TGTGGCTCCC 
GCTGGACAGA TCCAGTCTCA GCCGGAGCAG CTGCTCTTCA TCCTCGATGG TTTAGATGAA 
CTGGAATGTG TCTCAGAGGA GAAGAGGGCA GAGCTCTGTC TGCACCGGAG CCAGCAGCAG 
TCTGTGCAGA CACTGCTGGG CAGTTTGGTG GAGAAAACCA TATTTCCAGA GGCATCCTTG 
CTGATCACAG CTCGGACTGC AGCTCTGCAG AAGTTCATTC CTTCTTTGAA GCAGCCATGT 
TGGGTGGAGG TCCTGGGGTT CTCCGAGTCT TCCAGGAAGG ACTATTTCTA TGAATATTTC 
ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT AGCGTGGTTG AATCAAACCA AGCACTCTTG 
ACCATGTGCC TCACGCCCTT GGTGTCCTGG CTGGCCTGCA CTTGCCTGAA ACAGCAGATG 
GAGGCAGGGG AAGAACTCTC ACTGAAGTCG CAGACCATGA CAGCCCTCTA TCTGCGCTAC 
CTTGCCCAAG CTATCCAAGC TCAGCCCCTG GGCACTCAGC TGAGGGGCTT CTGTACTCTT 
GCTGCTGAGG GTATCTGTCA AGGAAAGACT CTGTTCAGTC CAGGGGACCT CAAGAAGCAT 
GGATTAGATG GGGTCATCAG TGCCACTCCC TTAAAGATGG ATGTTCTTCA AAAACACCCC 
ACATCTCTGA ACTACAGCTT CATTCACCTG TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC 
TTTGCCTTAG GGGACATTCG GGACAAAAGT GACCATCCTA ACAGCACTGG AAGCATGGAA 
AAGTTGGTAG AAGTATATGG GATCAATAAC CTCTTTGGGG CACCAACCGT GCATTTCCTA 
TCCGGCCTTT TGAGTGAGCA AGGAGCGAGA GAGATGGAGA ACATTTTCCA ATGCAAGCTG 
TCTTGGGAGA GAAAGCGGGA GTCACTGCTG CGGTGGGCTG AATTGGAACT CCGGCGTGAG 
CATTACTCTC TGCAATTGTT TCACTGTTTG TACGAGATTC AGGACGAGGA GTTCCTGACA 
CAAGCGATGG CCCATTTCCA AGGAATGAGA GTGTGCATCC GAACCAGCAT GGAGCTGCTC 
GTGTTCACTT TCTGCATTAG ATTCTGCAGC CACGTGTGGC GGCTTCAACT GAATGAGGGC 
AGACAACATC CAGGAGCATG CAGGCCCACC GACACAGTGC TGTTGAGCTG GGTCCCGGCC 
ACAGAAGCCT GCTGGCAGGT TCTCTGCTCT GTTCTCCAGG TCACTGGAAA CCTGAAGGAA 
CTGGACCTGA GTAGAAACAT CTTGAGCCGC TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG 
AGATGCCCTG GGTGCCAGCT AGAGACCCTG CGGTTGGCCA GCTGCAGCCT CACAGCTGAG 
AGCTGCAAGG ACATTGCCTC TGGGCTGAGC ACCAGCCAAA CCCTGATGCA ATTGGAATTG 
AGCTTCAACA CACTCCTGGA CGCAGGAGCT GAGCACCTTT GCCAGGGCCT GAGACAGCCA 
ACCTGCAAGC TGCAGCGACT GTTGCTGGCA GGCTGCGGCC TCACGTCTGG CTGCTGCCAG 
GACCTGGCCT CCATGCTCAG GGTCAGCCCC AGCCTGATGG AGCTAAACCT GCTGCAGAAT 
GACCTGGACG ACCTCGGCGT GAGGCTGCTC TGTGAAGGAC TCAGGCACCC TTCCTGCCAG 
CTCATATGCC TATGGCTGGA CCAGACTCAG CTGAGCGAGG AGGTGACCAA GATGCTGAGG 
GCCCTGGAGA GGCAGAAGCC TCAGCTGCTA GTCTCCGGGA TATGGAAGCC AAGTCTGAGG 
ATCCTTCACG AGGGCCCAGG TGGAGCAGAG ATGAGTGATA CATCCTCCCT CAAGCGGCAG 
AGACAAGCAT CAGAGGGCAG CTCTCCTCAA GCAGCACAGG TGAGGCTCTT CTGTCCGTCT 
TCTCCTGCCC CTCCGGAGGA CGAGCACATG GAGTCTCTGG GGACCGAGGA TGACCTCTGG 
GGCCCCACAG GGCCTGTGGC TACCGAGGCG GTTGACGAAG AGAGGAGCCT GTACCGCGTT 
CACTTGCCCA TGGCCGGCTT CTACCACTGG CCCAACACAG GTCTCCGCTT TGAGGTGAGA 
GGGCCAGTGA CCGTTGAAAT TGAATTCTGT GCCTGGGACC AGTTCCTGAA CAGGAATTTC 
CCACAGCGCA GCTGGCTGGC TGCAGGGCCT CTGTTTGACA TCAAGGCTGA ACCAGGCGCT 
GTGGCAGCCG TGTTCCTCCC TCACTTTGTG GCCTTCCGAG GGAACGATGT GGACATCTCC 
CAGTTTCAAG TGGCCCACTT CAAAGAGGAG GGGATGCTCC TGGAGAAGCC AGCCAGGGTG 
ACGCCATGTT ACGCCGTCCT GGAAAACCCC AGCTTCTCGC CCATGGGAGT TCTACTGAGG 
ATGGTCCAAA CTGCCCTGCG CTTCATTCCC ATCACCTCCA CTGTGTTGCT CTACCATTAC 
CTCCAGCCTG AAGAAGTTAC CTTCCACCTC TACCTGATCC CCAGCGATTG CTCCATTCAG 
AAGGCCATAG ACAATGAAGA AAAGATGTTC CAGTTTGTGC GAATTCACAA GCCACCCCCG 
CTGACCCCAC TCTACATGGG CTCCCGCTAC ATTGTGTCTG GTTCAGAGAA GCTGGAAATA 
CTGCCCGAGG AATTGGAGCT CTGCTATCGG AGCCCCAGAG AATCTCAGCT GTTCTCTGAG 
TTCTACGTTC GCCATTTGAG GTCAGGGATC AGGTTGCAAA TAAGAAGCAA GAAAGATGGG 
ACTGTGGTGT GGGAAGCCTT GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTC 
TCTCCAGGCC TCAGAGCCTC ACCTGACTCC CCAGCTGCCC TGAGATTGAG GCACTTTGTG 
GACCGACATC GGGAGCAGCT GGTGGCTCGA GTGACATCCG TGGACCCTAT CCTGGACAAG 
TTGCATGGAC AGGTGCTGAG CGAAGAACAG TATGAGGGGG TGCGGGCAGA GACCACTGCA 
CCAGGCCAGA TGCGGAGGCT GTTTGGTTTC AGCCGATCCT GGGACTGGGC CTGCAAAGAT 
CGCCTCTACC AAGCCCTGAA GGAGACCCAT CCTCACCTAA TCATGGAACT CTGGGAGAAG 
TGGGGCAGTG AAGAGACCAG GCCGGGCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010824036.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822338.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

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CloneID	OBa51513
Clone ID Related Accession (Same CDS sequence)	XM_010824036.1 , XM_010815958.1 , XM_010815958.2
Accession Version	XM_010824036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822338.1
CDS	472..4539
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010824036.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824036.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010815958.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814260.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa51513
Clone ID Related Accession (Same CDS sequence)	XM_010824036.1 , XM_010815958.1 , XM_010815958.2
Accession Version	XM_010815958.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814260.1
CDS	472..4539
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010815958.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815958.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010815958.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814260.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa51513
Clone ID Related Accession (Same CDS sequence)	XM_010824036.1 , XM_010815958.1 , XM_010815958.2
Accession Version	XM_010815958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814260.1
CDS	478..4545
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRACEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010815958.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815958.2

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGACC TTTCCCCAAG CATTTACCAA 
GGTTTTCCAA GTTCCCCATA TCATGAGTCA CCAAGCCAAG AGTTACCCAA TGACCCCACA 
TCCACCGCAG TCCTGAGAGC CTGTGAAATC CCACGTCATT CCTGTCCAGA GCCCAGAGAG 
AAGGAGGGTC CTGGGACTGT ATGGCCACTG GTTGAAACAT CTGGAAACAG CTGCACAGGA 
AGCAGACAGA GATACCAGGA TCAAAGGAGA GAGACACCTT GCTCCACCTG GTCATGGAAA 
AATGAAGATT TGCACCCAAA ATTCATGCAG TTGCTACTTC TACACACTCC TTACCACAAA 
GACTACGAGT CCTTGAACCG GGAAAGCTGG GATCACGGGG TGGTTGAGGG GCAAGGGCAT 
TTGATTGAGG TCAGAGACTT ATTTGGCTCA GACCTGGGTT CCCAGGAAGG GCCTCACACA 
GTCATATTGC ATGGAGTTGC TGGAATTGGG AAGTCCACAC TGGCCAGGCA CATAAGAAGA 
GCCTGGGAGG AGGGCCAGCT ATACAGGAAC CACTTCCAGC ATGTCTTCTA CTTCAACTGC 
AGAGAGCTGG CCACGTCCAA GCTGACAAGT CTGGAGGAAC TCATCACAAA AGACCAGTCC 
ACCACTGTGG CTCCCGCTGG ACAGATCCAG TCTCAGCCGG AGCAGCTGCT CTTCATCCTC 
GATGGTTTAG ATGAACTGGA ATGTGTCTCA GAGGAGAAGA GGGCAGAGCT CTGTCTGCAC 
CGGAGCCAGC AGCAGTCTGT GCAGACACTG CTGGGCAGTT TGGTGGAGAA AACCATATTT 
CCAGAGGCAT CCTTGCTGAT CACAGCTCGG ACTGCAGCTC TGCAGAAGTT CATTCCTTCT 
TTGAAGCAGC CATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCTTCCAG GAAGGACTAT 
TTCTATGAAT ATTTCACAGA GGAAAGCCAA GCAACTAGAG CCTTTAGCGT GGTTGAATCA 
AACCAAGCAC TCTTGACCAT GTGCCTCACG CCCTTGGTGT CCTGGCTGGC CTGCACTTGC 
CTGAAACAGC AGATGGAGGC AGGGGAAGAA CTCTCACTGA AGTCGCAGAC CATGACAGCC 
CTCTATCTGC GCTACCTTGC CCAAGCTATC CAAGCTCAGC CCCTGGGCAC TCAGCTGAGG 
GGCTTCTGTA CTCTTGCTGC TGAGGGTATC TGTCAAGGAA AGACTCTGTT CAGTCCAGGG 
GACCTCAAGA AGCATGGATT AGATGGGGTC ATCAGTGCCA CTCCCTTAAA GATGGATGTT 
CTTCAAAAAC ACCCCACATC TCTGAACTAC AGCTTCATTC ACCTGTGTTT CCAGGAGTTC 
TTTGCAGCAG TGTCCTTTGC CTTAGGGGAC ATTCGGGACA AAAGTGACCA TCCTAACAGC 
ACTGGAAGCA TGGAAAAGTT GGTAGAAGTA TATGGGATCA ATAACCTCTT TGGGGCACCA 
ACCGTGCATT TCCTATCCGG CCTTTTGAGT GAGCAAGGAG CGAGAGAGAT GGAGAACATT 
TTCCAATGCA AGCTGTCTTG GGAGAGAAAG CGGGAGTCAC TGCTGCGGTG GGCTGAATTG 
GAACTCCGGC GTGAGCATTA CTCTCTGCAA TTGTTTCACT GTTTGTACGA GATTCAGGAC 
GAGGAGTTCC TGACACAAGC GATGGCCCAT TTCCAAGGAA TGAGAGTGTG CATCCGAACC 
AGCATGGAGC TGCTCGTGTT CACTTTCTGC ATTAGATTCT GCAGCCACGT GTGGCGGCTT 
CAACTGAATG AGGGCAGACA ACATCCAGGA GCATGCAGGC CCACCGACAC AGTGCTGTTG 
AGCTGGGTCC CGGCCACAGA AGCCTGCTGG CAGGTTCTCT GCTCTGTTCT CCAGGTCACT 
GGAAACCTGA AGGAACTGGA CCTGAGTAGA AACATCTTGA GCCGCTCTGC AGTACAGAGT 
CTTGATGAGG CCCTGAGATG CCCTGGGTGC CAGCTAGAGA CCCTGCGGTT GGCCAGCTGC 
AGCCTCACAG CTGAGAGCTG CAAGGACATT GCCTCTGGGC TGAGCACCAG CCAAACCCTG 
ATGCAATTGG AATTGAGCTT CAACACACTC CTGGACGCAG GAGCTGAGCA CCTTTGCCAG 
GGCCTGAGAC AGCCAACCTG CAAGCTGCAG CGACTGTTGC TGGCAGGCTG CGGCCTCACG 
TCTGGCTGCT GCCAGGACCT GGCCTCCATG CTCAGGGTCA GCCCCAGCCT GATGGAGCTA 
AACCTGCTGC AGAATGACCT GGACGACCTC GGCGTGAGGC TGCTCTGTGA AGGACTCAGG 
CACCCTTCCT GCCAGCTCAT ATGCCTATGG CTGGACCAGA CTCAGCTGAG CGAGGAGGTG 
ACCAAGATGC TGAGGGCCCT GGAGAGGCAG AAGCCTCAGC TGCTAGTCTC CGGGATATGG 
AAGCCAAGTC TGAGGATCCT TCACGAGGGC CCAGGTGGAG CAGAGATGAG TGATACATCC 
TCCCTCAAGC GGCAGAGACA AGCATCAGAG GGCAGCTCTC CTCAAGCAGC ACAGGTGAGG 
CTCTTCTGTC CGTCTTCTCC TGCCCCTCCG GAGGACGAGC ACATGGAGTC TCTGGGGACC 
GAGGATGACC TCTGGGGCCC CACAGGGCCT GTGGCTACCG AGGCGGTTGA CGAAGAGAGG 
AGCCTGTACC GCGTTCACTT GCCCATGGCC GGCTTCTACC ACTGGCCCAA CACAGGTCTC 
CGCTTTGAGG TGAGAGGGCC AGTGACCGTT GAAATTGAAT TCTGTGCCTG GGACCAGTTC 
CTGAACAGGA ATTTCCCACA GCGCAGCTGG CTGGCTGCAG GGCCTCTGTT TGACATCAAG 
GCTGAACCAG GCGCTGTGGC AGCCGTGTTC CTCCCTCACT TTGTGGCCTT CCGAGGGAAC 
GATGTGGACA TCTCCCAGTT TCAAGTGGCC CACTTCAAAG AGGAGGGGAT GCTCCTGGAG 
AAGCCAGCCA GGGTGACGCC ATGTTACGCC GTCCTGGAAA ACCCCAGCTT CTCGCCCATG 
GGAGTTCTAC TGAGGATGGT CCAAACTGCC CTGCGCTTCA TTCCCATCAC CTCCACTGTG 
TTGCTCTACC ATTACCTCCA GCCTGAAGAA GTTACCTTCC ACCTCTACCT GATCCCCAGC 
GATTGCTCCA TTCAGAAGGC CATAGACAAT GAAGAAAAGA TGTTCCAGTT TGTGCGAATT 
CACAAGCCAC CCCCGCTGAC CCCACTCTAC ATGGGCTCCC GCTACATTGT GTCTGGTTCA 
GAGAAGCTGG AAATACTGCC CGAGGAATTG GAGCTCTGCT ATCGGAGCCC CAGAGAATCT 
CAGCTGTTCT CTGAGTTCTA CGTTCGCCAT TTGAGGTCAG GGATCAGGTT GCAAATAAGA 
AGCAAGAAAG ATGGGACTGT GGTGTGGGAA GCCTTGGTGA AGCCAGGAGA TCTCAGACGG 
GCAGTTACTC CAGTCTCTCC AGGCCTCAGA GCCTCACCTG ACTCCCCAGC TGCCCTGAGA 
TTGAGGCACT TTGTGGACCG ACATCGGGAG CAGCTGGTGG CTCGAGTGAC ATCCGTGGAC 
CCTATCCTGG ACAAGTTGCA TGGACAGGTG CTGAGCGAAG AACAGTATGA GGGGGTGCGG 
GCAGAGACCA CTGCACCAGG CCAGATGCGG AGGCTGTTTG GTTTCAGCCG ATCCTGGGAC 
TGGGCCTGCA AAGATCGCCT CTACCAAGCC CTGAAGGAGA CCCATCCTCA CCTAATCATG 
GAACTCTGGG AGAAGTGGGG CAGTGAAGAG ACCAGGCCGG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010815959.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814261.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

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CloneID	OBa51514
Clone ID Related Accession (Same CDS sequence)	XM_010815959.1 , XM_010824037.1 , XM_010815959.2
Accession Version	XM_010815959.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814261.1
CDS	472..4503
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010815959.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815959.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010824037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822339.1 Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

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CloneID	OBa51514
Clone ID Related Accession (Same CDS sequence)	XM_010815959.1 , XM_010824037.1 , XM_010815959.2
Accession Version	XM_010824037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646453.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822339.1
CDS	472..4503
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010824037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824037.1

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_010815959.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814261.1 Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

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CloneID	OBa51514
Clone ID Related Accession (Same CDS sequence)	XM_010815959.1 , XM_010824037.1 , XM_010815959.2
Accession Version	XM_010815959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814261.1
CDS	477..4508
Translation	MAGEARSLEDLEEKKKQKLKELRLWLPDEEPRLQSPEVVHAQPEKPRSRKTMTILSVMKEVQHECMAIHTKGPHVNEQGACTKTLEETDLNSALYSSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPVLNPLKWSSSPLIFASGYRKGSRQRYQDQRRETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGSQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELATSKLTSLEELITKDQSTTVAPAGQIQSQPEQLLFILDGLDELECVSEEKRAELCLHRSQQQSVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFSVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLKSQTMTALYLRYLAQAIQAQPLGTQLRGFCTLAAEGICQGKTLFSPGDLKKHGLDGVISATPLKMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDIRDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAREMENIFQCKLSWERKRESLLRWAELELRREHYSLQLFHCLYEIQDEEFLTQAMAHFQGMRVCIRTSMELLVFTFCIRFCSHVWRLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSRNILSRSAVQSLDEALRCPGCQLETLRLASCSLTAESCKDIASGLSTSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDDLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVTKMLRALERQKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPPEDEHMESLGTEDDLWGPTGPVATEAVDEERSLYRVHLPMAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQRSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKMFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVSPGLRASPDSPAALRLRHFVDRHREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRRLFGFSRSWDWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_010815959.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815959.2

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGAAG CCTGGAGGAC TTGGAGGAAA AGAAGAAACA GAAGCTGAAG 
GAGCTCCGGC TTTGGCTGCC TGATGAGGAA CCTCGCTTGC AGTCTCCTGA GGTGGTCCAT 
GCTCAACCAG AGAAGCCAAG GAGCCGGAAG ACCATGACGA TCCTCTCAGT GATGAAGGAG 
GTACAGCACG AGTGTATGGC CATCCACACC AAGGGACCAC ACGTGAACGA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATTCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAGTCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGTGCTCAAC CCACTGAAAT GGAGTTCGAG CCCCCTAATT 
TTCGCATCTG GATATAGGAA AGGAAGCAGA CAGAGATACC AGGATCAAAG GAGAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAC GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTTCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCACGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGACAGAT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGTGT CTCAGAGGAG 
AAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGT CTGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTGGGGTTC 
TCCGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA GCGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGCCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGCCTGAAA CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAAGTCGC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GCACTCAGCT GAGGGGCTTC TGTACTCTTG CTGCTGAGGG TATCTGTCAA 
GGAAAGACTC TGTTCAGTCC AGGGGACCTC AAGAAGCATG GATTAGATGG GGTCATCAGT 
GCCACTCCCT TAAAGATGGA TGTTCTTCAA AAACACCCCA CATCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATTCGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TCTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGAGAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAG 
TCACTGCTGC GGTGGGCTGA ATTGGAACTC CGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAATGAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGTGGCG GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCATGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAAGCCTG CTGGCAGGTT 
CTCTGCTCTG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TAGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGCCAGCTA 
GAGACCCTGC GGTTGGCCAG CTGCAGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCA CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACGA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGACCAAG ATGCTGAGGG CCCTGGAGAG GCAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGTGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGGCTCTTC TGTCCGTCTT CTCCTGCCCC TCCGGAGGAC 
GAGCACATGG AGTCTCTGGG GACCGAGGAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCGAGGCGG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCAT GGCCGGCTTC 
TACCACTGGC CCAACACAGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCGCAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCCGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGATGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGATGGGA CTGTGGTGTG GGAAGCCTTG 
GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTCT CTCCAGGCCT CAGAGCCTCA 
CCTGACTCCC CAGCTGCCCT GAGATTGAGG CACTTTGTGG ACCGACATCG GGAGCAGCTG 
GTGGCTCGAG TGACATCCGT GGACCCTATC CTGGACAAGT TGCATGGACA GGTGCTGAGC 
GAAGAACAGT ATGAGGGGGT GCGGGCAGAG ACCACTGCAC CAGGCCAGAT GCGGAGGCTG 
TTTGGTTTCA GCCGATCCTG GGACTGGGCC TGCAAAGATC GCCTCTACCA AGCCCTGAAG 
GAGACCCATC CTCACCTAAT CATGGAACTC TGGGAGAAGT GGGGCAGTGA AGAGACCAGG 
CCGGGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NLRP1
Entrez Gene ID	528166
Full Name	NLR family pyrin domain containing 1
General protein information	Preferred Names NLR family pyrin domain containing 1 Names NACHT, LRR and PYD domains-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	NLRP1	NP_127497.1
	NLRP1	NP_001107821.1
Pan troglodytes (chimpanzee)	NLRP1	XP_001167200.2
Canis lupus familiaris (dog)	NLRP1	XP_005619937.1
Bos taurus (cattle)	NLRP1	XP_003587454.2
Mus musculus (house mouse)	Nlrp1b	NP_001035786.1
Rattus norvegicus (Norway rat)	Nlrp1b	XP_006246908.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NLRP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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