WLS cDNA ORF clone, Bos taurus(cattle)

The following WLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01696 NM_001192651.1
Latest version!
Bos taurus wntless homolog (Drosophila) (WLS), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa17847 XM_005204461.3
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa232815 XM_010803446.3
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa232816 XM_015465038.2
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa90458 XM_010803446.2
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90459 XM_015465036.2
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90460 XM_015465038.1
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa216196 NM_001192651.2
Latest version!
Bos taurus Wnt ligand secretion mediator (WLS), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa90457 XM_015465034.2
Latest version!
Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OBa90459 XM_015465036.1 Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90457 XM_015465034.1 Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01696
    Clone ID Related Accession (Same CDS sequence) NM_001192651.1
    Accession Version NM_001192651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product protein wntless homolog
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03077220.1, AAFC03077216.1, AAFC03096080.1 and AAFC03096082.1. On Jul 17, 2010 this sequence version replaced XM_606535.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA CGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTGACTGA AGGCCATTGG
    AAGTGGGGTG GTGTCACAGT CCAAGTGAAC AGTGCCTTTT TCACTGGCAT CTATGGCATG
    TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTACGCGC CATCCCATAA GAACTATGGG
    GAAGACCAAT CCAACGGTGA TCTGGGTGTA CACAGTGGGG AAGAACTCCA GCTCACCACC
    ACTATCACCC ACGTGGACGG GCCCACTGAG ATCTACAAGT TGACCCGCAA GGAGGCCCAG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179580.1
    CDS220..1845
    Translation

    Target ORF information:

    RefSeq Version NM_001192651.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus wntless homolog (Drosophila) (WLS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA CGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTGACTGA AGGCCATTGG
    AAGTGGGGTG GTGTCACAGT CCAAGTGAAC AGTGCCTTTT TCACTGGCAT CTATGGCATG
    TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTACGCGC CATCCCATAA GAACTATGGG
    GAAGACCAAT CCAACGGTGA TCTGGGTGTA CACAGTGGGG AAGAACTCCA GCTCACCACC
    ACTATCACCC ACGTGGACGG GCCCACTGAG ATCTACAAGT TGACCCGCAA GGAGGCCCAG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17847
    Clone ID Related Accession (Same CDS sequence) XM_005204461.3
    Accession Version XM_005204461.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGGGGCT AATTTTCAGG
    TTCAAGTTCC TCATGCTTAT CACCTTGGCC TGTGCTGCCA TGACTGTCAT CTTCTTCATT
    GTTAGTCAGG TGACTGAAGG CCATTGGAAG TGGGGTGGTG TCACAGTCCA AGTGAACAGT
    GCCTTTTTCA CTGGCATCTA TGGCATGTGG AACCTGTATG TCTTTGCTTT GATGTTCTTG
    TACGCGCCAT CCCATAAGAA CTATGGGGAA GACCAATCCA ACGGTGATCT GGGTGTACAC
    AGTGGGGAAG AACTCCAGCT CACCACCACT ATCACCCACG TGGACGGGCC CACTGAGATC
    TACAAGTTGA CCCGCAAGGA GGCCCAGGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005204518.1
    CDS247..1599
    Translation

    Target ORF information:

    RefSeq Version XM_005204461.3
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGGGGCT AATTTTCAGG
    TTCAAGTTCC TCATGCTTAT CACCTTGGCC TGTGCTGCCA TGACTGTCAT CTTCTTCATT
    GTTAGTCAGG TGACTGAAGG CCATTGGAAG TGGGGTGGTG TCACAGTCCA AGTGAACAGT
    GCCTTTTTCA CTGGCATCTA TGGCATGTGG AACCTGTATG TCTTTGCTTT GATGTTCTTG
    TACGCGCCAT CCCATAAGAA CTATGGGGAA GACCAATCCA ACGGTGATCT GGGTGTACAC
    AGTGGGGAAG AACTCCAGCT CACCACCACT ATCACCCACG TGGACGGGCC CACTGAGATC
    TACAAGTTGA CCCGCAAGGA GGCCCAGGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232815
    Clone ID Related Accession (Same CDS sequence) XM_010803446.3
    Accession Version XM_010803446.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTTTCCTGT
    GGGCTTCCCT TGTGGCTCAG CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801748.2
    CDS250..1716
    Translation

    Target ORF information:

    RefSeq Version XM_010803446.3
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803446.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTTTCCTGT
    GGGCTTCCCT TGTGGCTCAG CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232816
    Clone ID Related Accession (Same CDS sequence) XM_015465038.2
    Accession Version XM_015465038.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015465038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTTTCCTG TGGGCTTCCC
    TTGTGGCTCA GCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320524.1
    CDS248..1645
    Translation

    Target ORF information:

    RefSeq Version XM_015465038.2
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTTTCCTG TGGGCTTCCC
    TTGTGGCTCA GCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90458
    Clone ID Related Accession (Same CDS sequence) XM_010803446.2
    Accession Version XM_010803446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTTTCCTGT
    GGGCTTCCCT TGTGGCTCAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801748.2
    CDS249..1715
    Translation

    Target ORF information:

    RefSeq Version XM_010803446.2
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTTTCCTGT
    GGGCTTCCCT TGTGGCTCAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90459
    Clone ID Related Accession (Same CDS sequence) XM_015465036.2 , XM_015465036.1
    Accession Version XM_015465036.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015465036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGATTTC AGAGACAAAG
    CAAGAACCTC AAATTCATCA GCAACATATT GGTGAAGCAA GCCAGGAGGA TCGGGGGCTA
    ATTTTCAGGT TCAAGTTCCT CATGCTTATC ACCTTGGCCT GTGCTGCCAT GACTGTCATC
    TTCTTCATTG TTAGTCAGGT GACTGAAGGC CATTGGAAGT GGGGTGGTGT CACAGTCCAA
    GTGAACAGTG CCTTTTTCAC TGGCATCTAT GGCATGTGGA ACCTGTATGT CTTTGCTTTG
    ATGTTCTTGT ACGCGCCATC CCATAAGAAC TATGGGGAAG ACCAATCCAA CGGTGATCTG
    GGTGTACACA GTGGGGAAGA ACTCCAGCTC ACCACCACTA TCACCCACGT GGACGGGCCC
    ACTGAGATCT ACAAGTTGAC CCGCAAGGAG GCCCAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320522.1
    CDS249..1670
    Translation

    Target ORF information:

    RefSeq Version XM_015465036.2
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465036.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGATTTC AGAGACAAAG
    CAAGAACCTC AAATTCATCA GCAACATATT GGTGAAGCAA GCCAGGAGGA TCGGGGGCTA
    ATTTTCAGGT TCAAGTTCCT CATGCTTATC ACCTTGGCCT GTGCTGCCAT GACTGTCATC
    TTCTTCATTG TTAGTCAGGT GACTGAAGGC CATTGGAAGT GGGGTGGTGT CACAGTCCAA
    GTGAACAGTG CCTTTTTCAC TGGCATCTAT GGCATGTGGA ACCTGTATGT CTTTGCTTTG
    ATGTTCTTGT ACGCGCCATC CCATAAGAAC TATGGGGAAG ACCAATCCAA CGGTGATCTG
    GGTGTACACA GTGGGGAAGA ACTCCAGCTC ACCACCACTA TCACCCACGT GGACGGGCCC
    ACTGAGATCT ACAAGTTGAC CCGCAAGGAG GCCCAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90459
    Clone ID Related Accession (Same CDS sequence) XM_015465036.2 , XM_015465036.1
    Accession Version XM_015465036.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGATTTC AGAGACAAAG
    CAAGAACCTC AAATTCATCA GCAACATATT GGTGAAGCAA GCCAGGAGGA TCGGGGGCTA
    ATTTTCAGGT TCAAGTTCCT CATGCTTATC ACCTTGGCCT GTGCTGCCAT GACTGTCATC
    TTCTTCATTG TTAGTCAGGT GACTGAAGGC CATTGGAAGT GGGGTGGTGT CACAGTCCAA
    GTGAACAGTG CCTTTTTCAC TGGCATCTAT GGCATGTGGA ACCTGTATGT CTTTGCTTTG
    ATGTTCTTGT ACGCGCCATC CCATAAGAAC TATGGGGAAG ACCAATCCAA CGGTGATCTG
    GGTGTACACA GTGGGGAAGA ACTCCAGCTC ACCACCACTA TCACCCACGT GGACGGGCCC
    ACTGAGATCT ACAAGTTGAC CCGCAAGGAG GCCCAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320522.1
    CDS248..1669
    Translation

    Target ORF information:

    RefSeq Version XM_015465036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGGAGA AAATGCAGAA
    GTTTCCATGG ATGTTTCTCT GGCTTACCGT GATGATATGT TTGCTGAGTG GACTGAAATG
    GCCCATGAGA GAGTGCCCCG GAAACTCAAA TGTACCTTCA CCTCCCCCAA GACCCTAGAA
    CATGAGGGCC GTTACTATGA ATGTGATGTC CTTCCTTTCA TGGAAATTGG GTCCGTGGCT
    CATAAGTATT ACCTTCTAAA TATCCGGCTG CCTGTGAATG AGAAGAAGAA AATCAACGTT
    GGGATTGGAG AGATAAAGGA TATTCGGCTG GTGGGAATTC ACCAGAATGG AGGCTTCACC
    AAGGTGTGGT TTGCCATGAA GACCTTCCTT ACACCCAGCA TCTTCATCAT TATGGTGTGG
    TATTGGAGGA GGATCACCAT GATGTCTCGA CCCCCAGTCC TCCTAGAAAA AGTCATCTTT
    GCCCTTGGGA TTTCCATGAC TTTTATCAAC ATCCCAGTGG AGTGGTTTTC CATTGGGTTT
    GACTGGACCT GGATGCTCTT GTTTGGTGAT ATCCGACAGG GCATCTTCTA CGCAATGCTT
    CTCTCCTTCT GGATCATCTT CTGTGGTGAG CACATGATGG ACCAGCATGA GCGGAATCAT
    ATAGCAGGGT ATTGGAAGCA AGTCGGACCA ATTGCTGTTG GCTCTTTCTG CCTTTTCATA
    TTTGACATGT GTGAGAGAGG GGTACAACTC ACGAATCCCT TCTACAGTAT CTGGACTACA
    GATGTCGGAA CAGAACTGGC TATGGCCTTC ATCATAGTGG CTGGGATCTG CCTCTGCCTC
    TACTTCCTGT TTCTGTGCTT CATGGTCTTT CAGGTTTTTC GGAACATCAG CGGGAAGCAA
    TCAAGCCTGC CAGCCATGAG CAAAGTCCGG CGGCTACACT ATGAGATTTC AGAGACAAAG
    CAAGAACCTC AAATTCATCA GCAACATATT GGTGAAGCAA GCCAGGAGGA TCGGGGGCTA
    ATTTTCAGGT TCAAGTTCCT CATGCTTATC ACCTTGGCCT GTGCTGCCAT GACTGTCATC
    TTCTTCATTG TTAGTCAGGT GACTGAAGGC CATTGGAAGT GGGGTGGTGT CACAGTCCAA
    GTGAACAGTG CCTTTTTCAC TGGCATCTAT GGCATGTGGA ACCTGTATGT CTTTGCTTTG
    ATGTTCTTGT ACGCGCCATC CCATAAGAAC TATGGGGAAG ACCAATCCAA CGGTGATCTG
    GGTGTACACA GTGGGGAAGA ACTCCAGCTC ACCACCACTA TCACCCACGT GGACGGGCCC
    ACTGAGATCT ACAAGTTGAC CCGCAAGGAG GCCCAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90460
    Clone ID Related Accession (Same CDS sequence) XM_015465038.1
    Accession Version XM_015465038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTTTCCTG TGGGCTTCCC
    TTGTGGCTCA GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320524.1
    CDS247..1644
    Translation

    Target ORF information:

    RefSeq Version XM_015465038.1
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTTTCCTG TGGGCTTCCC
    TTGTGGCTCA GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216196
    Clone ID Related Accession (Same CDS sequence) NM_001192651.2
    Accession Version NM_001192651.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-16
    Organism Bos taurus(cattle)
    Product protein wntless homolog
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On Jun 30, 2017 this sequence version replaced NM_001192651.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571255.20145.1, SRR5571262.13450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTGACTGA AGGCCATTGG
    AAGTGGGGTG GTGTCACAGT CCAAGTGAAC AGTGCCTTTT TCACTGGCAT CTATGGCATG
    TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTACGCGC CATCCCATAA GAACTATGGG
    GAAGACCAAT CCAACGGTGA TCTGGGTGTA CACAGTGGGG AAGAACTCCA GCTCACCACC
    ACTATCACCC ACGTGGACGG GCCCACTGAG ATCTACAAGT TGACCCGCAA GGAGGCCCAG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179580.1
    CDS220..1845
    Translation

    Target ORF information:

    RefSeq Version NM_001192651.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Wnt ligand secretion mediator (WLS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192651.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGGG GCTAATTTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG
    GCCTGTGCTG CCATGACTGT CATCTTCTTC ATTGTTAGTC AGGTGACTGA AGGCCATTGG
    AAGTGGGGTG GTGTCACAGT CCAAGTGAAC AGTGCCTTTT TCACTGGCAT CTATGGCATG
    TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTACGCGC CATCCCATAA GAACTATGGG
    GAAGACCAAT CCAACGGTGA TCTGGGTGTA CACAGTGGGG AAGAACTCCA GCTCACCACC
    ACTATCACCC ACGTGGACGG GCCCACTGAG ATCTACAAGT TGACCCGCAA GGAGGCCCAG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90457
    Clone ID Related Accession (Same CDS sequence) XM_015465034.1 , XM_015465034.2
    Accession Version XM_015465034.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTGACTGAA
    GGCCATTGGA AGTGGGGTGG TGTCACAGTC CAAGTGAACA GTGCCTTTTT CACTGGCATC
    TATGGCATGT GGAACCTGTA TGTCTTTGCT TTGATGTTCT TGTACGCGCC ATCCCATAAG
    AACTATGGGG AAGACCAATC CAACGGTGAT CTGGGTGTAC ACAGTGGGGA AGAACTCCAG
    CTCACCACCA CTATCACCCA CGTGGACGGG CCCACTGAGA TCTACAAGTT GACCCGCAAG
    GAGGCCCAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320520.1
    CDS248..1942
    Translation

    Target ORF information:

    RefSeq Version XM_015465034.1
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTGACTGAA
    GGCCATTGGA AGTGGGGTGG TGTCACAGTC CAAGTGAACA GTGCCTTTTT CACTGGCATC
    TATGGCATGT GGAACCTGTA TGTCTTTGCT TTGATGTTCT TGTACGCGCC ATCCCATAAG
    AACTATGGGG AAGACCAATC CAACGGTGAT CTGGGTGTAC ACAGTGGGGA AGAACTCCAG
    CTCACCACCA CTATCACCCA CGTGGACGGG CCCACTGAGA TCTACAAGTT GACCCGCAAG
    GAGGCCCAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90457
    Clone ID Related Accession (Same CDS sequence) XM_015465034.1 , XM_015465034.2
    Accession Version XM_015465034.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein wntless homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015465034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTGACTGAA
    GGCCATTGGA AGTGGGGTGG TGTCACAGTC CAAGTGAACA GTGCCTTTTT CACTGGCATC
    TATGGCATGT GGAACCTGTA TGTCTTTGCT TTGATGTTCT TGTACGCGCC ATCCCATAAG
    AACTATGGGG AAGACCAATC CAACGGTGAT CTGGGTGTAC ACAGTGGGGA AGAACTCCAG
    CTCACCACCA CTATCACCCA CGTGGACGGG CCCACTGAGA TCTACAAGTT GACCCGCAAG
    GAGGCCCAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320520.1
    CDS247..1941
    Translation

    Target ORF information:

    RefSeq Version XM_015465034.2
    Organism Bos taurus(cattle)
    Definition Bos taurus wntless Wnt ligand secretion mediator (WLS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465034.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTGGGG CAATTATAGA AAACATGAGC ACCAAGAAGC TGTGCATTGT TGGTGGGATT 
    CTGCTTGTGT TCCAAATCAT CGCCTTTCTG GTGGGAGGCT TGATTGCTCC AGGACCCACA
    ACGGCAGTAT CCTACATGTC AGTGAAATGT GTGGATGTCC GTAAAAACCA TCACAAGGCA
    AAATGGTTAA TGCCTTGGGG ACCCAACCAA TGTGACAAGA TCCGAGACAT CGAGGAAGCA
    ATTCCAAGGG AAATTGAAGC CAATGACATT GTGTTTTCTG TCCACATCCC CCTCCCTTCC
    ATGGAGATGA GTCCTTGGTT CCAGTTCATG CTGTTTATCC TGCAGCTGGA CATTGCATTC
    AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAGTTTCCA TGGATGTTTC TCTGGCTTAC
    CGTGATGATA TGTTTGCTGA GTGGACTGAA ATGGCCCATG AGAGAGTGCC CCGGAAACTC
    AAATGTACCT TCACCTCCCC CAAGACCCTA GAACATGAGG GCCGTTACTA TGAATGTGAT
    GTCCTTCCTT TCATGGAAAT TGGGTCCGTG GCTCATAAGT ATTACCTTCT AAATATCCGG
    CTGCCTGTGA ATGAGAAGAA GAAAATCAAC GTTGGGATTG GAGAGATAAA GGATATTCGG
    CTGGTGGGAA TTCACCAGAA TGGAGGCTTC ACCAAGGTGT GGTTTGCCAT GAAGACCTTC
    CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCT
    CGACCCCCAG TCCTCCTAGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACTTTTATC
    AACATCCCAG TGGAGTGGTT TTCCATTGGG TTTGACTGGA CCTGGATGCT CTTGTTTGGT
    GATATCCGAC AGGGCATCTT CTACGCAATG CTTCTCTCCT TCTGGATCAT CTTCTGTGGT
    GAGCACATGA TGGACCAGCA TGAGCGGAAT CATATAGCAG GGTATTGGAA GCAAGTCGGA
    CCAATTGCTG TTGGCTCTTT CTGCCTTTTC ATATTTGACA TGTGTGAGAG AGGGGTACAA
    CTCACGAATC CCTTCTACAG TATCTGGACT ACAGATGTCG GAACAGAACT GGCTATGGCC
    TTCATCATAG TGGCTGGGAT CTGCCTCTGC CTCTACTTCC TGTTTCTGTG CTTCATGGTC
    TTTCAGGTTT TTCGGAACAT CAGCGGGAAG CAATCAAGCC TGCCAGCCAT GAGCAAAGTC
    CGGCGGCTAC ACTATGAGAT TTCAGAGACA AAGCAAGAAC CTCAAATTCA TCAGCAACAT
    ATTGGTGAAG CAAGCCAGGA GGATCGGGGG CTAATTTTCA GGTTCAAGTT CCTCATGCTT
    ATCACCTTGG CCTGTGCTGC CATGACTGTC ATCTTCTTCA TTGTTAGTCA GGTGACTGAA
    GGCCATTGGA AGTGGGGTGG TGTCACAGTC CAAGTGAACA GTGCCTTTTT CACTGGCATC
    TATGGCATGT GGAACCTGTA TGTCTTTGCT TTGATGTTCT TGTACGCGCC ATCCCATAAG
    AACTATGGGG AAGACCAATC CAACGGTGAT CTGGGTGTAC ACAGTGGGGA AGAACTCCAG
    CTCACCACCA CTATCACCCA CGTGGACGGG CCCACTGAGA TCTACAAGTT GACCCGCAAG
    GAGGCCCAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.