WDFY4 cDNA ORF clone, Bos taurus(cattle)

The following WDFY4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WDFY4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01683 NM_001205945.1
Latest version!
Bos taurus WDFY family member 4 (WDFY4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa216362 NM_001205945.2
Latest version!
Bos taurus WDFY family member 4 (WDFY4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa231609 NM_001205945.3
Latest version!
Bos taurus WDFY family member 4 (WDFY4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa231607 XM_024986689.1
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa231608 XM_024986690.1
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa231609 XM_005226581.4
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa34353 XM_005226581.3
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa51433 XM_010820712.2
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa51434 XM_010820713.2
Latest version!
Bos taurus WDFY family member 4 (WDFY4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01683
    Clone ID Related Accession (Same CDS sequence) NM_001205945.1
    Accession Version NM_001205945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product WD repeat- and FYVE domain-containing protein 4
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03086049.1, AAFC03086047.1, AAFC03086051.1, AAFC03086048.1, AAFC03004585.1, AAFC03086050.1, AAFC03125821.1, AAFC03072170.1, AAFC03004583.1, AAFC03072166.1 and AAFC03004584.1. On Apr 20, 2011 this sequence version replaced XM_606192.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC GTGATGCTGT GCAACGTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTCGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTGCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACATCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192874.1
    CDS1..9567
    Translation

    Target ORF information:

    RefSeq Version NM_001205945.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC GTGATGCTGT GCAACGTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTCGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTGCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACATCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216362
    Clone ID Related Accession (Same CDS sequence) NM_001205945.2
    Accession Version NM_001205945.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02062220.1, DAAA02062221.1, DAAA02062222.1, DAAA02062223.1 and DAAA02062224.1. On Jun 30, 2017 this sequence version replaced NM_001205945.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822095, SAMN02822097 [ECO:0000350] ##Evidence-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
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    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
    5041
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    5161
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    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
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    7921
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    8221
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    8341
    8401
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    8521
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192874.2
    CDS1..9567
    Translation

    Target ORF information:

    RefSeq Version NM_001205945.2
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231609
    Clone ID Related Accession (Same CDS sequence) XM_005226581.4 , NM_001205945.3
    Accession Version NM_001205945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000028.1. On May 29, 2018 this sequence version replaced NM_001205945.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCCGAGGCCG AGGCTGAAGG TGACGACGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AATGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCG CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192874.3
    CDS1..9567
    Translation

    Target ORF information:

    RefSeq Version NM_001205945.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCCGAGGCCG AGGCTGAAGG TGACGACGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AATGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCG CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231607
    Clone ID Related Accession (Same CDS sequence) XM_024986689.1
    Accession Version XM_024986689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGA TCTCAAGTCC TCTGGCTCCT ATGCAGCCCC GAGTCATGCC CCCACATGCT
    GCCTCTCATG AAGCGTGTCC ACAGCCCTCG ACAGGTTCTG GAGACAGCGC CTGGGAACGG
    ACATTCCCGG CCAGTGTGCT GCAGTTCCTT GGCCTCGTGC ACAGCGCCTA CCCCCAGGAT
    CCAGCCTGGC GGGCCCCTGA GTTCCTCCAG ACCTTGGCTG CGGTCACCTT CCCCCTGGGA
    ACCCAGAAGG GGGCCGTGAC TGAGTCTGCC CAGAGCACCA GCAGCCCCGA GGCCGAGGCT
    GAAGGTGACG ACGCTGCGGA GGGTCTACAG GCTCCTGCCG AGTCACATCC CACCCGGAAA
    CAGCTGAAGG AATTCATGCA GCTACTCCTG AGGGAGTTCT TGCTTGGGGC TCCAAACCCC
    AAGCAGTGGG TGCCCCTGGA GGTGCTCCTG GAGGCTTCTC CAGACAATGC CACCACTCAA
    CAGAAGCGGG ACTTTCAGTC TGAGATCTTG CTTTCCACCA TGGAAATATT TCACGTGATG
    AGTGGAGGTC ATGCATCGAT GCTGAGAGGC AGTAGAGAGC CTCAGCCGGA TGCAGAAGCT
    GCTGCTGCTC CTTCCCTTGC CAACATCTCC CACTTCATCC AGAAGCTGGT GGAGAAGCTG
    TACAGTGGAA TGTTCTCGGC AGACCCCAGA CACATCCTTC TCTTCATCAC GGAGCACATC
    ATGGTGGTCA TCGAGAATGC CTCTGCTCAG AAGGACACTG CCATCAGTGC ACTGTACAGC
    AGTCTAAACA AAGTCATCCT TTACAGCCTC TCCAAGCCCC ACCAGTCCCT CTCCGAATGC
    CTCAGCCTCC TCAGCACCCT GGGCTTCCTT CAGGAGCACT GGGACATTCT GTTTGCCACC
    TATAATTCCA ACGCCAGCTT CCTCATGTGT CTCATGCATT GTCTGCTGCT GCTCAGCGCA
    AGAAGTTATC CAGAAGGATT TGGATTGGAA CCCAAGCCTA GAATGACTCC TTATCATCAA
    GTCTTCCTTT CTCCAAATGA AGAAGTGAGA GAAAAAAGAG GGGAAGAATT CCCAAGCTTG
    AGCGATGTTC AGCACAATAT CCAGAAGACT GTACGGATTC TCTGGCAGCA GCTCGTGGCA
    CAGAGGCGGC AGGAACTGGA GGATACCTTC AAAATCGATC TTTCTGTGAA ACCTGGAGAG
    AGTGAAGTGA AGATTGAGGA GATCACCCCA CTCTGGGAAG AGACAATGCT CAAGGCCTGG
    CAGCATTATC TAGCATCTGA AAGGAAGTCA CTGGCCAGTC GCTCAAACGT TGGACACCAC
    AGCAAAGTTT CTTCATGGAG TGGCAGCTTG TCCTCAGCCA TGAGGCTGAT GCCTGGGCGG
    CAGACCAAGG ACCCTGAGTG CAAAGCAGAG GACTTTGTGT CGTGTATTGA GAACTACAGA
    CGAAGAGGAC AAGAACTATA TGCGTCTCTA TACAAAGATC ACGTACAGAG ACGGAGATGT
    GGCAACATCA AGGCGGCCAA TGCGTGGACC AGAATCCAGG AGCAGCTTTT TGGGGAGCTG
    GGCTTGTGGC ATCAGATGGC AGAAGCCACA CCCTGCTCCC AGTGGGAACT GGATTGGAGA
    GAAGGACCGG CTCGTATGCG GAAACGCATC AGACGCTTAT CTCCACTGGA GGCCCCTAAT
    CAAGAAAGGC TCAAGGAAAG CCAAGACAGA AATGGCAATG TTTCTCAAAT AAACACTGAA
    CACCAAGACG AGCTGACACT GAAGGAAGGC GAGAGTGAGG CAGACGAGGT GGCGGGCTGC
    ACCCAGCTGA CTTTCTTCCC TGCCTTGCAC GAAAGCCTGC ACTCCGAAGA TTTCTTAGAG
    CTATGTCAGG AAAGACAAGT CATCTTACAA GAGCTTCTTG ATCATGAAAA GGTGACGCAG
    AAGTACTCTG TGGTCATCGT GCAGGGCCAC CTGGTCTGCG AAGGGCTCCT GCTCTTCGGC
    CAGCAGCACT TGTACATCTG CGAGAACTTC ACGCTGTCTC CCGTGGGTGA TGTCTACTGC
    ACCCGCCACT GCCTATCCAA CATTAGCGAC CCATTCATCT TCAACATGTG CAGCAAAGAC
    AGGTCCTCTG ACCATTACTC GTGCCAGCGC CACAGCTACG GCGACCTCAG GGAGCTCCGG
    CAGGCCCGCT TCCTCCTGCA GGACATTGCC CTGGAGGTCT TTTTCCAAAA TGGATATTCC
    AAGTTTCTTG TCTTCCACAA CAGTGATCGG AGTAAGGTAT TCAAAAGCTT CTGCTCTTTC
    CAACCCAGCT TGAAAGGAAA AGGCTTCACA GAGGACACGC TCAATCTAAG GAGGTACCCC
    AGCTCCGACA GGACCATGCT GCAGAAGTGG CAGAAAAGAG ACATCAGCAA CTTCGAGTAC
    CTCATGTACC TCAACACCCT GGCTGGGAGG ACCTACAATG ACTACATGCA GTATCCAGTG
    TTCCCCTGGG TCCTGGCCGA CTACACCTCA CAGACACTGA ACCTGATGAA TCCCAAGACT
    TTCCGCGATC TTTCTAAGCC CATGGGCGCT CAGACCAAGG AAAGGAAGCT GAAATTCATC
    CAGAGGTTTA AAGAGGTTGA GAAGACTGAA GGAGACGTGA CCGCCCGGTG CCACTACTGC
    ACCCACTACT CCTCGGCCAT CATTGTCGCC TCCTACCTGG TCCGGATGCC ACCCTTCACC
    CAGGCCTTCT GCTCTCTGCA GGGCGGAAGC TTCGATGTGG CAGATAGAAT GTTCCACAGT
    GTGAAGAATG CGTGGGAATC GGCCTCCAGA GAGAACATGA GTGATGTCAG AGAGCTGACC
    CCGGAGTTCT TCTACCTGCC TGAGTTCCTG ACCAACTGCA ACGCCCTGGA GTTTGGCTGC
    ATGCAGGACG GAACAGCACT GGGCGATGTT CAGCTCCCTC CCTGGGCTGA TGGAGACCCC
    CGGAAATTCA TCAGCCTGCA CAGACAAGCT TTGGAAAGTG ATTTTGTCAG CGCCAACCTG
    CACCACTGGA TAGACCTCAT TTTTGGGTAC AAGCAGCAGG GGTCAGCCGC AGTGGAGGCT
    GTTAACACCT TCCATCCCTA CTTCTATGGC GACAAAGTGG ACCTCAGCAG CATCAGCGAC
    CCCCTGATCA GAAGCACCAT CCTGGGGTTC GTCAGCAACT TTGGACAGGT GCCCAAACAG
    CTCTTTACTA AACCCCATCC GGCCCGGACT GCCGCAGGGA AGCCTTCCCC TGGCAAGGAC
    GTCTCCACGC CTGCCAGCCT GCCCGGGCAC CCGCAGCCCT TCTTGTACAG CCTGCAGTCG
    CTGAGGCCCT CCCAGGTCAC CGTCAAAGAT ATGTACCTCT TCTCCCTAGG CTCGGAATCC
    CCCAAAGGAG CCATCGGCCA CATCGTTCCT ACGGAGAAGA CCATCCTGGC CGTGGAGACA
    AACAAGATGC TGCTGCCCCC ACACTGGAGC AGGACCTTCA GCTGGGGCTT TGACGACTTC
    AGCTGCTGCC TGGGCAGCTA CGGCTCTGAC AAGATCTTGG TGACGTTTGA GAACTTGGCC
    GCCTGGGGCC GCTGTCTGTG TGCTGCATGC CCGTCCCCCA CGACCATCAT CACCGCCGGG
    ACCAGCGCCG TGGTGTGTGT TTGGGAGCTC AGCATGACCA AAGGCCGCGC CACAGGCCTG
    CGCCTCAAGC AGGCCTTGTA CGGACACACG CAGGCTGTCA CGTGCCTGGC AGCATCAGTC
    ACCTTCAGCC TCCTGGTGAG TGGCTCGCAA GACCGCACAT GCATCCTGTG GGACCTGGAC
    CACCTCACCC ACGTAGCCCG CCTGCCCGCC CACAGGGACG GCGTATCTGC TGTTGCCATC
    AGCGACGTCT CGGGCACCAT CGTCTCCTGT GCGGGAGCCC ACTTGTCCCT GTGGGATGTC
    AACGGACAGC CACTGGCCAG CATTACCACG GCCTGGGGCC CAGAAGGAGC CATCACCTGC
    TGCTACGTGG TGGAGGGGCC AGCGTGGGAC ACAAGCCATG TCATCATCAC TGGGAGTCGG
    GATGGCATGG TCCGGATCTG GAAGACTGAA GATGTGAAGA TGTCCGTTCC CGGGCAGGCA
    GCACCGGAGG AGCCATGGGC TCCATCATCT CCAAGCCCCA GAGTTCACCG GTGGGAGAAG
    AATCTTGCCC TTTGCAGAGA GCTGGACGTC AGCGTGGCTT TGACAGGGAA ACCCAGCAAG
    GCCAACTCTG CAGTGACAGC TCTGGCCGTG TCCAGAAACC AGACCAAACT CCTGGTCGGC
    GATGAGAAGG GAAGAATATT CTGCTGGTCT GCAGAGGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842457.1
    CDS235..9876
    Translation

    Target ORF information:

    RefSeq Version XM_024986689.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGA TCTCAAGTCC TCTGGCTCCT ATGCAGCCCC GAGTCATGCC CCCACATGCT
    GCCTCTCATG AAGCGTGTCC ACAGCCCTCG ACAGGTTCTG GAGACAGCGC CTGGGAACGG
    ACATTCCCGG CCAGTGTGCT GCAGTTCCTT GGCCTCGTGC ACAGCGCCTA CCCCCAGGAT
    CCAGCCTGGC GGGCCCCTGA GTTCCTCCAG ACCTTGGCTG CGGTCACCTT CCCCCTGGGA
    ACCCAGAAGG GGGCCGTGAC TGAGTCTGCC CAGAGCACCA GCAGCCCCGA GGCCGAGGCT
    GAAGGTGACG ACGCTGCGGA GGGTCTACAG GCTCCTGCCG AGTCACATCC CACCCGGAAA
    CAGCTGAAGG AATTCATGCA GCTACTCCTG AGGGAGTTCT TGCTTGGGGC TCCAAACCCC
    AAGCAGTGGG TGCCCCTGGA GGTGCTCCTG GAGGCTTCTC CAGACAATGC CACCACTCAA
    CAGAAGCGGG ACTTTCAGTC TGAGATCTTG CTTTCCACCA TGGAAATATT TCACGTGATG
    AGTGGAGGTC ATGCATCGAT GCTGAGAGGC AGTAGAGAGC CTCAGCCGGA TGCAGAAGCT
    GCTGCTGCTC CTTCCCTTGC CAACATCTCC CACTTCATCC AGAAGCTGGT GGAGAAGCTG
    TACAGTGGAA TGTTCTCGGC AGACCCCAGA CACATCCTTC TCTTCATCAC GGAGCACATC
    ATGGTGGTCA TCGAGAATGC CTCTGCTCAG AAGGACACTG CCATCAGTGC ACTGTACAGC
    AGTCTAAACA AAGTCATCCT TTACAGCCTC TCCAAGCCCC ACCAGTCCCT CTCCGAATGC
    CTCAGCCTCC TCAGCACCCT GGGCTTCCTT CAGGAGCACT GGGACATTCT GTTTGCCACC
    TATAATTCCA ACGCCAGCTT CCTCATGTGT CTCATGCATT GTCTGCTGCT GCTCAGCGCA
    AGAAGTTATC CAGAAGGATT TGGATTGGAA CCCAAGCCTA GAATGACTCC TTATCATCAA
    GTCTTCCTTT CTCCAAATGA AGAAGTGAGA GAAAAAAGAG GGGAAGAATT CCCAAGCTTG
    AGCGATGTTC AGCACAATAT CCAGAAGACT GTACGGATTC TCTGGCAGCA GCTCGTGGCA
    CAGAGGCGGC AGGAACTGGA GGATACCTTC AAAATCGATC TTTCTGTGAA ACCTGGAGAG
    AGTGAAGTGA AGATTGAGGA GATCACCCCA CTCTGGGAAG AGACAATGCT CAAGGCCTGG
    CAGCATTATC TAGCATCTGA AAGGAAGTCA CTGGCCAGTC GCTCAAACGT TGGACACCAC
    AGCAAAGTTT CTTCATGGAG TGGCAGCTTG TCCTCAGCCA TGAGGCTGAT GCCTGGGCGG
    CAGACCAAGG ACCCTGAGTG CAAAGCAGAG GACTTTGTGT CGTGTATTGA GAACTACAGA
    CGAAGAGGAC AAGAACTATA TGCGTCTCTA TACAAAGATC ACGTACAGAG ACGGAGATGT
    GGCAACATCA AGGCGGCCAA TGCGTGGACC AGAATCCAGG AGCAGCTTTT TGGGGAGCTG
    GGCTTGTGGC ATCAGATGGC AGAAGCCACA CCCTGCTCCC AGTGGGAACT GGATTGGAGA
    GAAGGACCGG CTCGTATGCG GAAACGCATC AGACGCTTAT CTCCACTGGA GGCCCCTAAT
    CAAGAAAGGC TCAAGGAAAG CCAAGACAGA AATGGCAATG TTTCTCAAAT AAACACTGAA
    CACCAAGACG AGCTGACACT GAAGGAAGGC GAGAGTGAGG CAGACGAGGT GGCGGGCTGC
    ACCCAGCTGA CTTTCTTCCC TGCCTTGCAC GAAAGCCTGC ACTCCGAAGA TTTCTTAGAG
    CTATGTCAGG AAAGACAAGT CATCTTACAA GAGCTTCTTG ATCATGAAAA GGTGACGCAG
    AAGTACTCTG TGGTCATCGT GCAGGGCCAC CTGGTCTGCG AAGGGCTCCT GCTCTTCGGC
    CAGCAGCACT TGTACATCTG CGAGAACTTC ACGCTGTCTC CCGTGGGTGA TGTCTACTGC
    ACCCGCCACT GCCTATCCAA CATTAGCGAC CCATTCATCT TCAACATGTG CAGCAAAGAC
    AGGTCCTCTG ACCATTACTC GTGCCAGCGC CACAGCTACG GCGACCTCAG GGAGCTCCGG
    CAGGCCCGCT TCCTCCTGCA GGACATTGCC CTGGAGGTCT TTTTCCAAAA TGGATATTCC
    AAGTTTCTTG TCTTCCACAA CAGTGATCGG AGTAAGGTAT TCAAAAGCTT CTGCTCTTTC
    CAACCCAGCT TGAAAGGAAA AGGCTTCACA GAGGACACGC TCAATCTAAG GAGGTACCCC
    AGCTCCGACA GGACCATGCT GCAGAAGTGG CAGAAAAGAG ACATCAGCAA CTTCGAGTAC
    CTCATGTACC TCAACACCCT GGCTGGGAGG ACCTACAATG ACTACATGCA GTATCCAGTG
    TTCCCCTGGG TCCTGGCCGA CTACACCTCA CAGACACTGA ACCTGATGAA TCCCAAGACT
    TTCCGCGATC TTTCTAAGCC CATGGGCGCT CAGACCAAGG AAAGGAAGCT GAAATTCATC
    CAGAGGTTTA AAGAGGTTGA GAAGACTGAA GGAGACGTGA CCGCCCGGTG CCACTACTGC
    ACCCACTACT CCTCGGCCAT CATTGTCGCC TCCTACCTGG TCCGGATGCC ACCCTTCACC
    CAGGCCTTCT GCTCTCTGCA GGGCGGAAGC TTCGATGTGG CAGATAGAAT GTTCCACAGT
    GTGAAGAATG CGTGGGAATC GGCCTCCAGA GAGAACATGA GTGATGTCAG AGAGCTGACC
    CCGGAGTTCT TCTACCTGCC TGAGTTCCTG ACCAACTGCA ACGCCCTGGA GTTTGGCTGC
    ATGCAGGACG GAACAGCACT GGGCGATGTT CAGCTCCCTC CCTGGGCTGA TGGAGACCCC
    CGGAAATTCA TCAGCCTGCA CAGACAAGCT TTGGAAAGTG ATTTTGTCAG CGCCAACCTG
    CACCACTGGA TAGACCTCAT TTTTGGGTAC AAGCAGCAGG GGTCAGCCGC AGTGGAGGCT
    GTTAACACCT TCCATCCCTA CTTCTATGGC GACAAAGTGG ACCTCAGCAG CATCAGCGAC
    CCCCTGATCA GAAGCACCAT CCTGGGGTTC GTCAGCAACT TTGGACAGGT GCCCAAACAG
    CTCTTTACTA AACCCCATCC GGCCCGGACT GCCGCAGGGA AGCCTTCCCC TGGCAAGGAC
    GTCTCCACGC CTGCCAGCCT GCCCGGGCAC CCGCAGCCCT TCTTGTACAG CCTGCAGTCG
    CTGAGGCCCT CCCAGGTCAC CGTCAAAGAT ATGTACCTCT TCTCCCTAGG CTCGGAATCC
    CCCAAAGGAG CCATCGGCCA CATCGTTCCT ACGGAGAAGA CCATCCTGGC CGTGGAGACA
    AACAAGATGC TGCTGCCCCC ACACTGGAGC AGGACCTTCA GCTGGGGCTT TGACGACTTC
    AGCTGCTGCC TGGGCAGCTA CGGCTCTGAC AAGATCTTGG TGACGTTTGA GAACTTGGCC
    GCCTGGGGCC GCTGTCTGTG TGCTGCATGC CCGTCCCCCA CGACCATCAT CACCGCCGGG
    ACCAGCGCCG TGGTGTGTGT TTGGGAGCTC AGCATGACCA AAGGCCGCGC CACAGGCCTG
    CGCCTCAAGC AGGCCTTGTA CGGACACACG CAGGCTGTCA CGTGCCTGGC AGCATCAGTC
    ACCTTCAGCC TCCTGGTGAG TGGCTCGCAA GACCGCACAT GCATCCTGTG GGACCTGGAC
    CACCTCACCC ACGTAGCCCG CCTGCCCGCC CACAGGGACG GCGTATCTGC TGTTGCCATC
    AGCGACGTCT CGGGCACCAT CGTCTCCTGT GCGGGAGCCC ACTTGTCCCT GTGGGATGTC
    AACGGACAGC CACTGGCCAG CATTACCACG GCCTGGGGCC CAGAAGGAGC CATCACCTGC
    TGCTACGTGG TGGAGGGGCC AGCGTGGGAC ACAAGCCATG TCATCATCAC TGGGAGTCGG
    GATGGCATGG TCCGGATCTG GAAGACTGAA GATGTGAAGA TGTCCGTTCC CGGGCAGGCA
    GCACCGGAGG AGCCATGGGC TCCATCATCT CCAAGCCCCA GAGTTCACCG GTGGGAGAAG
    AATCTTGCCC TTTGCAGAGA GCTGGACGTC AGCGTGGCTT TGACAGGGAA ACCCAGCAAG
    GCCAACTCTG CAGTGACAGC TCTGGCCGTG TCCAGAAACC AGACCAAACT CCTGGTCGGC
    GATGAGAAGG GAAGAATATT CTGCTGGTCT GCAGAGGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231608
    Clone ID Related Accession (Same CDS sequence) XM_024986690.1
    Accession Version XM_024986690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGA TCTCAAGTCC TCTGGCTCCT ATGCAGCCCC GAGTCATGCC CCCACATGCT
    GCCTCTCATG AAGCGTGTCC ACAGCCCTCG ACAGGTTCTG GAGACAGCGC CTGGGAACGG
    ACATTCCCGG CCAGTGTGCT GCAGTTCCTT GGCCTCGTGC ACAGCGCCTA CCCCCAGGAT
    CCAGCCTGGC GGGCCCCTGA GTTCCTCCAG ACCTTGGCTG CGGTCACCTT CCCCCTGGGA
    ACCCAGAAGG GGGCCGTGAC TGAGTCTGCC CAGAGCACCA GCAGCCCCGA GGCCGAGGCT
    GAAGGTGACG ACGCTGCGGA GGGTCTACAG GCTCCTGCCG AGTCACATCC CACCCGGAAA
    CAGCTGAAGG AATTCATGCA GCTACTCCTG AGGGAGTTCT TGCTTGGGGC TCCAAACCCC
    AAGCAGTGGG TGCCCCTGGA GGTGCTCCTG GAGGCTTCTC CAGACAATGC CACCACTCAA
    CAGAAGCGGG ACTTTCAGTC TGAGATCTTG CTTTCCACCA TGGAAATATT TCACGTGATG
    AGTGGAGGTC ATGCATCGAT GCTGAGAGGC AGTAGAGAGC CTCAGCCGGA TGCAGAAGCT
    GCTGCTGCTC CTTCCCTTGC CAACATCTCC CACTTCATCC AGAAGCTGGT GGAGAAGCTG
    TACAGTGGAA TGTTCTCGGC AGACCCCAGA CACATCCTTC TCTTCATCAC GGAGCACATC
    ATGGTGGTCA TCGAGAATGC CTCTGCTCAG AAGGACACTG CCATCAGTGC ACTGTACAGC
    AGTCTAAACA AAGTCATCCT TTACAGCCTC TCCAAGCCCC ACCAGTCCCT CTCCGAATGC
    CTCAGCCTCC TCAGCACCCT GGGCTTCCTT CAGGAGCACT GGGACATTCT GTTTGCCACC
    TATAATTCCA ACGCCAGCTT CCTCATGTGT CTCATGCATT GTCTGCTGCT GCTCAGCGCA
    AGAAGTTATC CAGAAGGATT TGGATTGGAA CCCAAGCCTA GAATGACTCC TTATCATCAA
    GTCTTCCTTT CTCCAAATGA AGAAGTGAGA GAAAAAAGAG GGGAAGAATT CCCAAGCTTG
    AGCGATGTTC AGCACAATAT CCAGAAGACT GTACGGATTC TCTGGCAGCA GCTCGTGGCA
    CAGAGGCGGC AGGAACTGGA GGATACCTTC AAAATCGATC TTTCTGTGAA ACCTGGAGAG
    AGTGAAGTGA AGATTGAGGA GATCACCCCA CTCTGGGAAG AGACAATGCT CAAGGCCTGG
    CAGCATTATC TAGCATCTGA AAGGAAGTCA CTGGCCAGTC GCTCAAACGT TGGACACCAC
    AGCAAAGTTT CTTCATGGAG TGGCAGCTTG TCCTCAGCCA TGAGGCTGAT GCCTGGGCGG
    CAGACCAAGG ACCCTGAGTG CAAAGCAGAG GACTTTGTGT CGTGTATTGA GAACTACAGA
    CGAAGAGGAC AAGAACTATA TGCGTCTCTA TACAAAGATC ACGTACAGAG ACGGAGATGT
    GGCAACATCA AGGCGGCCAA TGCGTGGACC AGAATCCAGG AGCAGCTTTT TGGGGAGCTG
    GGCTTGTGGC ATCAGATGGC AGAAGCCACA CCCTGCTCCC AGTGGGAACT GGATTGGAGA
    GAAGGACCGG CTCGTATGCG GAAACGCATC AGACGCTTAT CTCCACTGGA GGCCCCTAAT
    CAAGAAAGGC TCAAGGAAAG CCAAGACAGA AATGGCAATG TTTCTCAAAT AAACACTGAA
    CACCAAGACG AGCTGACACT GAAGGAAGGC GAGAGTGAGG CAGACGAGGT GGCGGGCTGC
    ACCCAGCTGA CTTTCTTCCC TGCCTTGCAC GAAAGCCTGC ACTCCGAAGA TTTCTTAGAG
    CTATGTCAGG AAAGACAAGT CATCTTACAA GAGCTTCTTG ATCATGAAAA GGTGACGCAG
    AAGTACTCTG TGGTCATCGT GCAGGGCCAC CTGGTCTGCG AAGGGCTCCT GCTCTTCGGC
    CAGCAGCACT TGTACATCTG CGAGAACTTC ACGCTGTCTC CCGTGGGTGA TGTCTACTGC
    ACCCGCCACT GCCTATCCAA CATTAGCGAC CCATTCATCT TCAACATGTG CAGCAAAGAC
    AGGTCCTCTG ACCATTACTC GTGCCAGCGC CACAGCTACG GCGACCTCAG GGAGCTCCGG
    CAGGCCCGCT TCCTCCTGCA GGACATTGCC CTGGAGGTCT TTTTCCAAAA TGGATATTCC
    AAGTTTCTTG TCTTCCACAA CAGTGATCGG AGTAAGGTAT TCAAAAGCTT CTGCTCTTTC
    CAACCCAGCT TGAAAGGAAA AGGCTTCACA GAGGACACGC TCAATCTAAG GAGGTACCCC
    AGCTCCGACA GGACCATGCT GCAGAAGTGG CAGAAAAGAG ACATCAGCAA CTTCGAGTAC
    CTCATGTACC TCAACACCCT GGCTGGGAGG ACCTACAATG ACTACATGCA GTATCCAGTG
    TTCCCCTGGG TCCTGGCCGA CTACACCTCA CAGACACTGA ACCTGATGAA TCCCAAGACT
    TTCCGCGATC TTTCTAAGCC CATGGGCGCT CAGACCAAGG AAAGGAAGCT GAAATTCATC
    CAGAGGTTTA AAGAGGTTGA GAAGACTGAA GGAGACGTGA CCGCCCGGTG CCACTACTGC
    ACCCACTACT CCTCGGCCAT CATTGTCGCC TCCTACCTGG TCCGGATGCC ACCCTTCACC
    CAGGCCTTCT GCTCTCTGCA GGGCGGAAGC TTCGATGTGG CAGATAGAAT GTTCCACAGT
    GTGAAGAATG CGTGGGAATC GGCCTCCAGA GAGAACATGA GTGATGTCAG AGAGCTGACC
    CCGGAGTTCT TCTACCTGCC TGAGTTCCTG ACCAACTGCA ACGCCCTGGA GTTTGGCTGC
    ATGCAGGACG GAACAGCACT GGGCGATGTT CAGCTCCCTC CCTGGGCTGA TGGAGACCCC
    CGGAAATTCA TCAGCCTGCA CAGACAAGCT TTGGAAAGTG ATTTTGTCAG CGCCAACCTG
    CACCACTGGA TAGACCTCAT TTTTGGGTAC AAGCAGCAGG GGTCAGCCGC AGTGGAGGCT
    GTTAACACCT TCCATCCCTA CTTCTATGGC GACAAAGTGG ACCTCAGCAG CATCAGCGAC
    CCCCTGATCA GAAGCACCAT CCTGGGGTTC GTCAGCAACT TTGGACAGGT GCCCAAACAG
    CTCTTTACTA AACCCCATCC GGCCCGGACT GCCGCAGGGA AGCCTTCCCC TGGCAAGGAC
    GTCTCCACGC CTGCCAGCCT GCCCGGGCAC CCGCAGCCCT TCTTGTACAG CCTGCAGTCG
    CTGAGGCCCT CCCAGGTCAC CGTCAAAGGC TCGGAATCCC CCAAAGGAGC CATCGGCCAC
    ATCGTTCCTA CGGAGAAGAC CATCCTGGCC GTGGAGACAA ACAAGATGCT GCTGCCCCCA
    CACTGGAGCA GGACCTTCAG CTGGGGCTTT GACGACTTCA GCTGCTGCCT GGGCAGCTAC
    GGCTCTGACA AGATCTTGGT GACGTTTGAG AACTTGGCCG CCTGGGGCCG CTGTCTGTGT
    GCTGCATGCC CGTCCCCCAC GACCATCATC ACCGCCGGGA CCAGCGCCGT GGTGTGTGTT
    TGGGAGCTCA GCATGACCAA AGGCCGCGCC ACAGGCCTGC GCCTCAAGCA GGCCTTGTAC
    GGACACACGC AGGCTGTCAC GTGCCTGGCA GCATCAGTCA CCTTCAGCCT CCTGGTGAGT
    GGCTCGCAAG ACCGCACATG CATCCTGTGG GACCTGGACC ACCTCACCCA CGTAGCCCGC
    CTGCCCGCCC ACAGGGACGG CGTATCTGCT GTTGCCATCA GCGACGTCTC GGGCACCATC
    GTCTCCTGTG CGGGAGCCCA CTTGTCCCTG TGGGATGTCA ACGGACAGCC ACTGGCCAGC
    ATTACCACGG CCTGGGGCCC AGAAGGAGCC ATCACCTGCT GCTACGTGGT GGAGGGGCCA
    GCGTGGGACA CAAGCCATGT CATCATCACT GGGAGTCGGG ATGGCATGGT CCGGATCTGG
    AAGACTGAAG ATGTGAAGAT GTCCGTTCCC GGGCAGGCAG CACCGGAGGA GCCATGGGCT
    CCATCATCTC CAAGCCCCAG AGTTCACCGG TGGGAGAAGA ATCTTGCCCT TTGCAGAGAG
    CTGGACGTCA GCGTGGCTTT GACAGGGAAA CCCAGCAAGG CCAACTCTGC AGTGACAGCT
    CTGGCCGTGT CCAGAAACCA GACCAAACTC CTGGTCGGCG ATGAGAAGGG AAGAATATTC
    TGCTGGTCTG CAGAGGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842458.1
    CDS235..9855
    Translation

    Target ORF information:

    RefSeq Version XM_024986690.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGA TCTCAAGTCC TCTGGCTCCT ATGCAGCCCC GAGTCATGCC CCCACATGCT
    GCCTCTCATG AAGCGTGTCC ACAGCCCTCG ACAGGTTCTG GAGACAGCGC CTGGGAACGG
    ACATTCCCGG CCAGTGTGCT GCAGTTCCTT GGCCTCGTGC ACAGCGCCTA CCCCCAGGAT
    CCAGCCTGGC GGGCCCCTGA GTTCCTCCAG ACCTTGGCTG CGGTCACCTT CCCCCTGGGA
    ACCCAGAAGG GGGCCGTGAC TGAGTCTGCC CAGAGCACCA GCAGCCCCGA GGCCGAGGCT
    GAAGGTGACG ACGCTGCGGA GGGTCTACAG GCTCCTGCCG AGTCACATCC CACCCGGAAA
    CAGCTGAAGG AATTCATGCA GCTACTCCTG AGGGAGTTCT TGCTTGGGGC TCCAAACCCC
    AAGCAGTGGG TGCCCCTGGA GGTGCTCCTG GAGGCTTCTC CAGACAATGC CACCACTCAA
    CAGAAGCGGG ACTTTCAGTC TGAGATCTTG CTTTCCACCA TGGAAATATT TCACGTGATG
    AGTGGAGGTC ATGCATCGAT GCTGAGAGGC AGTAGAGAGC CTCAGCCGGA TGCAGAAGCT
    GCTGCTGCTC CTTCCCTTGC CAACATCTCC CACTTCATCC AGAAGCTGGT GGAGAAGCTG
    TACAGTGGAA TGTTCTCGGC AGACCCCAGA CACATCCTTC TCTTCATCAC GGAGCACATC
    ATGGTGGTCA TCGAGAATGC CTCTGCTCAG AAGGACACTG CCATCAGTGC ACTGTACAGC
    AGTCTAAACA AAGTCATCCT TTACAGCCTC TCCAAGCCCC ACCAGTCCCT CTCCGAATGC
    CTCAGCCTCC TCAGCACCCT GGGCTTCCTT CAGGAGCACT GGGACATTCT GTTTGCCACC
    TATAATTCCA ACGCCAGCTT CCTCATGTGT CTCATGCATT GTCTGCTGCT GCTCAGCGCA
    AGAAGTTATC CAGAAGGATT TGGATTGGAA CCCAAGCCTA GAATGACTCC TTATCATCAA
    GTCTTCCTTT CTCCAAATGA AGAAGTGAGA GAAAAAAGAG GGGAAGAATT CCCAAGCTTG
    AGCGATGTTC AGCACAATAT CCAGAAGACT GTACGGATTC TCTGGCAGCA GCTCGTGGCA
    CAGAGGCGGC AGGAACTGGA GGATACCTTC AAAATCGATC TTTCTGTGAA ACCTGGAGAG
    AGTGAAGTGA AGATTGAGGA GATCACCCCA CTCTGGGAAG AGACAATGCT CAAGGCCTGG
    CAGCATTATC TAGCATCTGA AAGGAAGTCA CTGGCCAGTC GCTCAAACGT TGGACACCAC
    AGCAAAGTTT CTTCATGGAG TGGCAGCTTG TCCTCAGCCA TGAGGCTGAT GCCTGGGCGG
    CAGACCAAGG ACCCTGAGTG CAAAGCAGAG GACTTTGTGT CGTGTATTGA GAACTACAGA
    CGAAGAGGAC AAGAACTATA TGCGTCTCTA TACAAAGATC ACGTACAGAG ACGGAGATGT
    GGCAACATCA AGGCGGCCAA TGCGTGGACC AGAATCCAGG AGCAGCTTTT TGGGGAGCTG
    GGCTTGTGGC ATCAGATGGC AGAAGCCACA CCCTGCTCCC AGTGGGAACT GGATTGGAGA
    GAAGGACCGG CTCGTATGCG GAAACGCATC AGACGCTTAT CTCCACTGGA GGCCCCTAAT
    CAAGAAAGGC TCAAGGAAAG CCAAGACAGA AATGGCAATG TTTCTCAAAT AAACACTGAA
    CACCAAGACG AGCTGACACT GAAGGAAGGC GAGAGTGAGG CAGACGAGGT GGCGGGCTGC
    ACCCAGCTGA CTTTCTTCCC TGCCTTGCAC GAAAGCCTGC ACTCCGAAGA TTTCTTAGAG
    CTATGTCAGG AAAGACAAGT CATCTTACAA GAGCTTCTTG ATCATGAAAA GGTGACGCAG
    AAGTACTCTG TGGTCATCGT GCAGGGCCAC CTGGTCTGCG AAGGGCTCCT GCTCTTCGGC
    CAGCAGCACT TGTACATCTG CGAGAACTTC ACGCTGTCTC CCGTGGGTGA TGTCTACTGC
    ACCCGCCACT GCCTATCCAA CATTAGCGAC CCATTCATCT TCAACATGTG CAGCAAAGAC
    AGGTCCTCTG ACCATTACTC GTGCCAGCGC CACAGCTACG GCGACCTCAG GGAGCTCCGG
    CAGGCCCGCT TCCTCCTGCA GGACATTGCC CTGGAGGTCT TTTTCCAAAA TGGATATTCC
    AAGTTTCTTG TCTTCCACAA CAGTGATCGG AGTAAGGTAT TCAAAAGCTT CTGCTCTTTC
    CAACCCAGCT TGAAAGGAAA AGGCTTCACA GAGGACACGC TCAATCTAAG GAGGTACCCC
    AGCTCCGACA GGACCATGCT GCAGAAGTGG CAGAAAAGAG ACATCAGCAA CTTCGAGTAC
    CTCATGTACC TCAACACCCT GGCTGGGAGG ACCTACAATG ACTACATGCA GTATCCAGTG
    TTCCCCTGGG TCCTGGCCGA CTACACCTCA CAGACACTGA ACCTGATGAA TCCCAAGACT
    TTCCGCGATC TTTCTAAGCC CATGGGCGCT CAGACCAAGG AAAGGAAGCT GAAATTCATC
    CAGAGGTTTA AAGAGGTTGA GAAGACTGAA GGAGACGTGA CCGCCCGGTG CCACTACTGC
    ACCCACTACT CCTCGGCCAT CATTGTCGCC TCCTACCTGG TCCGGATGCC ACCCTTCACC
    CAGGCCTTCT GCTCTCTGCA GGGCGGAAGC TTCGATGTGG CAGATAGAAT GTTCCACAGT
    GTGAAGAATG CGTGGGAATC GGCCTCCAGA GAGAACATGA GTGATGTCAG AGAGCTGACC
    CCGGAGTTCT TCTACCTGCC TGAGTTCCTG ACCAACTGCA ACGCCCTGGA GTTTGGCTGC
    ATGCAGGACG GAACAGCACT GGGCGATGTT CAGCTCCCTC CCTGGGCTGA TGGAGACCCC
    CGGAAATTCA TCAGCCTGCA CAGACAAGCT TTGGAAAGTG ATTTTGTCAG CGCCAACCTG
    CACCACTGGA TAGACCTCAT TTTTGGGTAC AAGCAGCAGG GGTCAGCCGC AGTGGAGGCT
    GTTAACACCT TCCATCCCTA CTTCTATGGC GACAAAGTGG ACCTCAGCAG CATCAGCGAC
    CCCCTGATCA GAAGCACCAT CCTGGGGTTC GTCAGCAACT TTGGACAGGT GCCCAAACAG
    CTCTTTACTA AACCCCATCC GGCCCGGACT GCCGCAGGGA AGCCTTCCCC TGGCAAGGAC
    GTCTCCACGC CTGCCAGCCT GCCCGGGCAC CCGCAGCCCT TCTTGTACAG CCTGCAGTCG
    CTGAGGCCCT CCCAGGTCAC CGTCAAAGGC TCGGAATCCC CCAAAGGAGC CATCGGCCAC
    ATCGTTCCTA CGGAGAAGAC CATCCTGGCC GTGGAGACAA ACAAGATGCT GCTGCCCCCA
    CACTGGAGCA GGACCTTCAG CTGGGGCTTT GACGACTTCA GCTGCTGCCT GGGCAGCTAC
    GGCTCTGACA AGATCTTGGT GACGTTTGAG AACTTGGCCG CCTGGGGCCG CTGTCTGTGT
    GCTGCATGCC CGTCCCCCAC GACCATCATC ACCGCCGGGA CCAGCGCCGT GGTGTGTGTT
    TGGGAGCTCA GCATGACCAA AGGCCGCGCC ACAGGCCTGC GCCTCAAGCA GGCCTTGTAC
    GGACACACGC AGGCTGTCAC GTGCCTGGCA GCATCAGTCA CCTTCAGCCT CCTGGTGAGT
    GGCTCGCAAG ACCGCACATG CATCCTGTGG GACCTGGACC ACCTCACCCA CGTAGCCCGC
    CTGCCCGCCC ACAGGGACGG CGTATCTGCT GTTGCCATCA GCGACGTCTC GGGCACCATC
    GTCTCCTGTG CGGGAGCCCA CTTGTCCCTG TGGGATGTCA ACGGACAGCC ACTGGCCAGC
    ATTACCACGG CCTGGGGCCC AGAAGGAGCC ATCACCTGCT GCTACGTGGT GGAGGGGCCA
    GCGTGGGACA CAAGCCATGT CATCATCACT GGGAGTCGGG ATGGCATGGT CCGGATCTGG
    AAGACTGAAG ATGTGAAGAT GTCCGTTCCC GGGCAGGCAG CACCGGAGGA GCCATGGGCT
    CCATCATCTC CAAGCCCCAG AGTTCACCGG TGGGAGAAGA ATCTTGCCCT TTGCAGAGAG
    CTGGACGTCA GCGTGGCTTT GACAGGGAAA CCCAGCAAGG CCAACTCTGC AGTGACAGCT
    CTGGCCGTGT CCAGAAACCA GACCAAACTC CTGGTCGGCG ATGAGAAGGG AAGAATATTC
    TGCTGGTCTG CAGAGGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231609
    Clone ID Related Accession (Same CDS sequence) XM_005226581.4 , NM_001205945.3
    Accession Version XM_005226581.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCCGAGGCCG AGGCTGAAGG TGACGACGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AATGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCG CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226638.2
    CDS235..9801
    Translation

    Target ORF information:

    RefSeq Version XM_005226581.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226581.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACTATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAATAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTATG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCA
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCACAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACTGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCAT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACG
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ATGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCCGTT
    ACCAGCGTTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGTCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCCGAGGCCG AGGCTGAAGG TGACGACGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AATGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCG CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACATGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCGTA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34353
    Clone ID Related Accession (Same CDS sequence) XM_005226581.3
    Accession Version XM_005226581.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2221
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    2641
    2701
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    3001
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    3121
    3181
    3241
    3301
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    4141
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    4261
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    4441
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    8701
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    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226638.1
    CDS240..9806
    Translation

    Target ORF information:

    RefSeq Version XM_005226581.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226581.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAATT TCTAGGCCTT GGAATTGCTT CACCTCCATC GGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCCG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51433
    Clone ID Related Accession (Same CDS sequence) XM_010820712.2
    Accession Version XM_010820712.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010819014.1
    CDS240..9806
    Translation

    Target ORF information:

    RefSeq Version XM_010820712.2
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820712.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGATATGTA CCTCTTCTCC CTAGGCTCGG AATCCCCCAA AGGAGCCATC
    GGCCACATCG TTCCTACGGA GAAGACCATC CTGGCCGTGG AGACAAACAA GATGCTGCTG
    CCCCCACACT GGAGCAGGAC CTTCAGCTGG GGCTTTGACG ACTTCAGCTG CTGCCTGGGC
    AGCTACGGCT CTGACAAGAT CTTGGTGACG TTTGAGAACT TGGCCGCCTG GGGCCGCTGT
    CTGTGTGCTG CATGCCCGTC CCCCACGACC ATCATCACCT CCGGGACCAG CGCCGTGGTG
    TGTGTTTGGG AGCTCAGCAT GACCAAAGGC CGCGCCACAG GCCTGCGCCT CAAGCAGGCC
    TTGTACGGAC ACACGCAGGC TGTCACGTGC CTGGCAGCAT CAGTCACCTT CAGCCTCCTG
    GTGAGTGGCT CGCAAGACCG CACGTGCATC CTGTGGGACC TGGACCACCT CACCCACGTA
    GCCCGCCTGC CCGCCCACAG GGACGGCATA TCTGCTGTTG CCATCAGCGA CGTCTCGGGC
    ACCATCGTCT CCTGTGCGGG AGCCCACTTG TCCCTGTGGG ATGTCAACGG ACAGCCACTG
    GCCAGCATTA CCACGGCCTG GGGCCCAGAA GGAGCCATCA CCTGCTGCTA CGTGGTGGAG
    GGGCCAGCGT GGGACACAAG CCATGTCATC ATCACTGGGA GTCGGGATGG CATGGTCCGG
    ATCTGGAAGA CTGAAGATGT GAAGATGTCC GTTCCCGGGC AGGCAGCACC GGAGGAGCCA
    TGGGCTCCAT CATCTCCAAG CCCCAGAGTT CACCGGTGGG AGAAGAATCT TGCCCTTTGC
    AGAGAGCTGG ACGTCAGCGT GGCTTTGACA GGGAAACCCA GCAAGGCCAA CTCTGCAGTG
    ACAGCTCTGG CCGTGTCCAG AAACCAGACC AAACTCCTGG TCGGCGATGA GAAGGGAAGA
    ATATTCTGCT GGTCTGCAGA GGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51434
    Clone ID Related Accession (Same CDS sequence) XM_010820713.2
    Accession Version XM_010820713.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat- and FYVE domain-containing protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1141
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    1321
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    1501
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    9001
    9061
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    9181
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    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGGCTCGGA ATCCCCCAAA GGAGCCATCG GCCACATCGT TCCTACGGAG
    AAGACCATCC TGGCCGTGGA GACAAACAAG ATGCTGCTGC CCCCACACTG GAGCAGGACC
    TTCAGCTGGG GCTTTGACGA CTTCAGCTGC TGCCTGGGCA GCTACGGCTC TGACAAGATC
    TTGGTGACGT TTGAGAACTT GGCCGCCTGG GGCCGCTGTC TGTGTGCTGC ATGCCCGTCC
    CCCACGACCA TCATCACCTC CGGGACCAGC GCCGTGGTGT GTGTTTGGGA GCTCAGCATG
    ACCAAAGGCC GCGCCACAGG CCTGCGCCTC AAGCAGGCCT TGTACGGACA CACGCAGGCT
    GTCACGTGCC TGGCAGCATC AGTCACCTTC AGCCTCCTGG TGAGTGGCTC GCAAGACCGC
    ACGTGCATCC TGTGGGACCT GGACCACCTC ACCCACGTAG CCCGCCTGCC CGCCCACAGG
    GACGGCATAT CTGCTGTTGC CATCAGCGAC GTCTCGGGCA CCATCGTCTC CTGTGCGGGA
    GCCCACTTGT CCCTGTGGGA TGTCAACGGA CAGCCACTGG CCAGCATTAC CACGGCCTGG
    GGCCCAGAAG GAGCCATCAC CTGCTGCTAC GTGGTGGAGG GGCCAGCGTG GGACACAAGC
    CATGTCATCA TCACTGGGAG TCGGGATGGC ATGGTCCGGA TCTGGAAGAC TGAAGATGTG
    AAGATGTCCG TTCCCGGGCA GGCAGCACCG GAGGAGCCAT GGGCTCCATC ATCTCCAAGC
    CCCAGAGTTC ACCGGTGGGA GAAGAATCTT GCCCTTTGCA GAGAGCTGGA CGTCAGCGTG
    GCTTTGACAG GGAAACCCAG CAAGGCCAAC TCTGCAGTGA CAGCTCTGGC CGTGTCCAGA
    AACCAGACCA AACTCCTGGT CGGCGATGAG AAGGGAAGAA TATTCTGCTG GTCTGCAGAG
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010819015.1
    CDS240..9785
    Translation

    Target ORF information:

    RefSeq Version XM_010820713.2
    Organism Bos taurus(cattle)
    Definition Bos taurus WDFY family member 4 (WDFY4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820713.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGGAGGCGA AAGATGTCTC AGAGGCTGAA GACAGAGCTG AGGATCCAGG TTCCAAAAAC 
    AAAGGCCAGC CTGATGCTGT GCAACCTGAT TTCCCACCAG GAGGGCAGAC CCCCGGCCCC
    ACTGCCCTCT GGGAGATGCT GGAAAGGAAG TTTCTGGAAT ACCAGCAGTT GGCTTCCAGG
    AGCCCGGCTG AACAACAGAA GAGCCTGTTG AATCTTCTCC CACTGTTCCT AAAGGCCTGG
    GAACACTCCG TGGGGATCAT CTGCTTTCCC AGTCTTCAAA GGCTGGCTGA AGATGTTTCC
    AACCAGTTTG CCCAAGAAAT ACAGAAGGCA CTTGTGGGAA AGCCTGCAGA GCAAGCACGG
    GTGGCAGCCG GGAAGCTGCT GCAGTGGAAG GGAGATGCGG ACGTGCATCA GGAAGGTCAC
    CTACTCCTGA AGTCGGTGTA TGTGCTCACG GGGACGGATG CCGAGACGCT GGGCAGAGTT
    GCTGAATCTG GGCTCCCAGC CCTGCTCCTC CAGTGCCTTT ACCTGTTCTT TGTTTTTCCT
    CTGGAAACGG ATGAGCTTTT AGAGAGTGAT GTTCAAGTTC AGAGGATGTT TGTGCAGATG
    TTGCTCAACA TTTGCGGAGA GGCTCAGAGT CTGGAGGGAC TTCTCTCAGG AAATGAACTC
    CAGTCTCTCA TGATTGCCAC GACATGTCTT CGAGAGCACA GCTGCTGCTT TTGGAAGGAG
    CCCACCTTCT GTGTGCTGAA GACCATCTCC AAGGCCCAGA ACGTCGGCGT CATCCAGTAC
    CTGCAGGCCA TGGATTGCAT CAAGATCTCC CTCCAGAACC TCTCCAGGCT TGTTGACACC
    CTCCCCGCTC CTGAGGTGAG CGAGGTAGTG AGCCTCGTCT TGGGCTTTGT GAAGGACTCC
    TATCCCATCT CCTCAGCTCT GTTCCTGGAG TTTGAGACTG GGGAGGGCTA CCCTCTGCTG
    CTCAAAGTGT TACTTCGGTA CGACGGACTG ACCCAGAGCG AGGCTGACCC CCACCTGGAG
    GAGCTCCTCG GGCTGGTGGT GTGGCTGACA ACCTGTGGGA GGTCAGAGCT GAAGGTGTTC
    GACAGCATCA CTTACCCGCA GCTTGAAGGC TTCAAGTTCC ATCAGGAGGC TTCCGGGGTG
    ACTGTGAAGA ATCTTCAGGC CTTTCAAGTC CTGCAGAATG TTTTCCACAA AGTCAGCGAC
    CCCATCATCT GCACCAAAGT CCTGTCGTCC ATCAGGACCA TGTGGACCTG GAACCCCCGC
    AACTTCTTCC TCCTGGAGTG GACCCTGCAG CCCATCTCGC AGTTTGTGGA GATCGTGCCC
    CTGAAGCCGA CCCTGGTGCA GAGGCACTTC TTCCAGATGC TGGAGGCCCT GGTGTTCGAG
    CTGCGTTACG TGCCCCACGA GATCCTGCGG AAGGTGCAGC GCCTGATCCA GGAGAGCCCG
    GAGTCGCTGT GTACCCTCGT GGCCCTGCAG AGTGTCCTCA GGATTGCTGG TGGCGACCCG
    CTCTTCACTG ACATTTTCCG GGACTCGGGG CTCCTGGGTC TGCTGCTGGC CCAGCTACGG
    AAGCACGCCA AGATCCTGAG GAAGTCAGGA AACAAAGAGG TCAATCCTGG AGTTCAAGAT
    ACAGAAAGAG AGCTGACTTG TGTGATGCTG AAAACGGTGG TCACGCTTCT GAAAGGGTCA
    GTTAGGAATG CAGTTGTCCT GAAAGACCAC GGCATGGTGC CCTTCATCAA GATCTTCCTG
    GATGGCGAGT GCTACCGGGC AGCCTCGCTC AGCATCTTGG AGCAGCTCTC AGTCATCAAC
    GCCGAGGAGT ACATGAGCAT CATTGTGGGC GCTTTGTGCT CGTCCACTCA AGGGGAAATA
    CAGCTGAAAC TGGACCTCCT GAAGTCTCTG CTCCGGATCC TGGAGACCCC CAAAGGCCGC
    GCTGCTTTCA GAGTCTCCAG TGGGTTCAAC GGGCTGCTGT CCCTTCTCTC TGACCTGGAG
    GGGTCTCTCC AGGAGCCCCC ACTGCAGACG TGGGGGGCAG TGTCCCCCAG CCAGACCCTG
    GACCTCGTCC TGCACACGCT CTGTGCTTTG TCTGCTGCAC TGCACCTGGA CCCAGTCAAC
    GGTGACTTCT TTAGGAGGAC CGGGCTCTTT GAGAAGCTGG CCGAGGACCT CTGCTTGCTG
    GGCTGTTTTG GGGCCCTGGA GGAAGAGGAC GCCCCTCCGC CCTCCCGGGA GGACACCAAG
    GCCAGGCCGT TTGCTGATCT GCTGAGTGCG GCCTTTTCCT CTGCCAGCCT GCTCCCACCC
    AAGATCCAGA GCTGCCTGCA GATCCTCAGC TTTCTGGACA GCATGGCCCG AGGTACCCTC
    CACCTGCGCA GGGACCTGAA GGACGCCCCG AGGACCAGGC AGGAGCTAGC GGTGGACCCT
    CAGAGGGCAG AACCTGGCAG TGACCCCCAG GGAAGCTTCA CGCAGTGGCC AGACCTGGAA
    GAGAGGACAG ATGACAGTGA TGCTGTGATC ATGCACCCCG GGGTCCTGTG CATCATGGTG
    AGGCTGCTGC CTCGCTTGTA CCACGAAGAC CACCCTCAGC TTTCCAGGGA GATCCAGTGC
    TCCATGGCCA GTCACATCCA GTCCATGGTG AAGTCGGAGA AGAACCGTCA GGTCATGTGC
    GAGGCGGGGA TGCTCAGGAC CCTCATGACT TCTTGCCACA AGGCCCTGAC CACCAGCAGC
    AGCCCCTTGC ACTCGGGCCT CATCAGGATT TTTGAAAAGC TTGCTTCCCA GGCCATCGAA
    CCAGACGTGT TAAGACAGTT TCTAGGCCTT GGAATTGCTT CACCTCCATC TGCTGCAACG
    AAAAAACTCA GTTCATCTCA TGCGTGTGGA GGGGACTCTG GATGCCCAGG GTCACAGGCA
    GCGATGCCAG TGCCTGCTGA GGGTTCTGCA GTGGCCCCTG GGGATGCCGG CCCTCACCCA
    GCAAGCACAC AGGCCCTCAA GCCCATGGGG CTGTCCCAGA CCTCGACCAC CACCCTTCAG
    ACAGCACTGA GCCTCATCTC CATGACCTCC CCCCGCAACC TGCAGCCTCA GAGGGCGGCC
    CTGGCTCCAT CCTTCGTGGA ATTCGACATG TCCGTGGAAG GTTACGGCTG TTTGTTTGTT
    CCGACTCTGG CTACGGTGAT GGGAACCAGC ACCGAGTACT CAGTCTCTGG AGGGATTGGG
    ACAGGTGCTG CCAGGTCCTT CCCTCCGCCC GGGGGCCTGA GCTTCTCCTG CTGGTTCCTG
    GTCGGCAGGC ACAGCGCAGC CGCGGAGGGC CACCCACTGC GCTTCCTGAC CCTGGTGCGC
    CACCTGGCCA GGACCGAGCA GCCCTTCGTC TGCTTCTCTG TCAGCCTCTG CCCAGAAGAC
    CTCTCCCTGG TTGTGTCTAC AGAAGAGAAA GAGTTTCAGC CCCTGGATGT CATGGAACTC
    GAGGATGACC CTGAGCCTTC TGCAGGACGC CAGCTTCGGG TCAGGTGTGG CCAGCTGCTG
    GCTTGTGGTC AGTGGCACCA CCTGGCTGTG GTCGTCACCA AGGAAATGAA AAGGAATTGT
    ACAGTCTTCA CCTACCTGGA TGGACAGGTC ATCGGCTCAG CCAAGATGTT GTACATTCAG
    GCCTTGCCAG GACCATTCCT TTCGATGGAT CCATCCTCGT TTGTGGATGT TTATGGATAT
    ATTGCTACCC CTCGAGTTTG GAAACAAAAG TCATTACTAA TATGGCGTCT GGGCCCCACA
    TACTTCTTTG AAGAAGCCAT CTCAATGGAA ACTCTGGAAG TTATCAACAA ACTTGGCCCA
    AGATACTGTG GTAACTTCCA GGCTGTGCAT GTCCAAGGAG AGGATCCTGG TGTGGAAGCA
    ACGCCCCTGG TTGCAGAGGA AAAGGTTTCC TTCGGCCTTC ACATAGCCAG CTCCTCTGTT
    ACCAGCATTG CAGACATTAG AAGCACGTAC AATGAGGTGG ACAGCCGCCT GATTGCCAAA
    GAGATGAACA TTTCATCCCG TGACAGTGTC GTGCCTGTGT TCTTGCTAAG AAACTGTGCT
    GGTCACCTCT CGGGTTCTCT TCGAACCATT GGAGCTGTTG CTGTGGGCCA ATTAGGGGTA
    AGAGTGTTCC ACTCCAGCCC TGCTGCCAGC AGCCTGGACT TCATTGGCGG GCCTGCCATC
    CTCCTGGGCC TCATCTCCTT AGCAACGGAC GACCACAGCA TGTACGCGGC TGTGAAAGTC
    CTGCACTTGG TTCTGACCAG CAACGTCATG TGTGACCGCC TGATGCAGCA CATCTGTGGG
    TACCAGATAA TGGCATTTCT CCTGAGGAAA AAGACCTCTT TTCTGAACCA TCGCATTTTT
    CAGCTGATCC TGTCTATCAC TGGCACTGCA GAACTGGGCT TTGGACCCTC CGTAATCACC
    AACGTTGGTG TCTTTCAGCA CATCCTCTGC AATTTCGAGC TCTGGATGAA TGCTGCCGAC
    AATTTGGAGC TCTCTCTCTT TTCCCATCTA GTGGAAATCC TTCAGTCAAC AAGGGAAGGA
    CCCCGGAATG CTGAAGTCGC TCACCAGGCA CAGCTTATTC CCAAGCTCGT CTTCCTCTTC
    AACGAGCCAG GCCTCACGCC CTCCAAGATG CGCACCGTCA TTGCCATCCT GGGCTGTCAG
    CTGCGGGGCC ACTTCAGCCT CCAGGACCTG CTTAGAATCG CTCTGTTTGT GGTGTACACC
    CTCAAGCCTT CGTCGTTGAA CGAGAAGCAG ATCTGTGTGG ACAGAGCCCC GGACCCTTCC
    CTGCCCGCTG GAAGCCAAAC ATCTGGAAAG ACAATCTGGC TCAGAAACCA GTTGCTGGAG
    ATGCTGCTCA GCGTAATATC TTCTTCCCAA CTTCATCTGT CCTCTGAGAC AAAGGAAGAG
    ATGTTTCTGA ACCTGGGGTC CGACTGGTTC CTGCTCTTCG TGCAGGGCCA CATGCACCCC
    AGCACAGTGG TGCTGGGCTT GAAGCTGCTG CTGCACTTTT TGTCAAGCCC CTCCCTCCGA
    GGGCGATTTA AAGACGGCCT GCCTGCAGGC TCCTGGGTGG AACGCAGCTC CGAGGGCATG
    GACATCGTGA TGGATAACCT GAAGAGCCAT CCTCCAACGC CTGACCAGAG CCCCTGCCTG
    CTTCCTGGGT TCCGGGTCTT GAGCGACTTT TTGGCCCACC ATGTTCATAT TCCAGAAGTC
    TACCTCATCG TGTCCACCTT CTTCCTCCAG ACACCCCTCA CAGAGCTGAC GGACGGGCCC
    AAGGACAGCC TGGATGCCAT GCTTCAGTGG CTCTTGCATA AACACCACAG GGAAGAAGTC
    CTCCGGGCAG GGCTGTGCAC TGAAGGCGCC CTGCTGCTCC TAGAGATGCT GAAAGCCACC
    ATGAGCCAGC CCTCGACAGG TTCTGGAGAC AGCGCCTGGG AACGGACATT CCCGGCCAGT
    GTGCTGCAGT TCCTTGGCCT CGTGCACAGC GCCTACCCCC AGGATCCAGC CTGGCGGGCC
    CCTGAGTTCC TCCAGACCTT GGCTGCGGTC ACCTTCCCCC TGGGAACCCA GAAGGGGGCC
    GTGACTGAGT CTGCCCAGAG CACCAGCAGC CCTGAGGCCG AGGCTGAAGG TGACGATGCT
    GCGGAGGGTC TACAGGCTCC TGCCGAGTCA CATCCCACCC GGAAACAGCT GAAGGAATTC
    ATGCAGCTAC TCCTGAGGGA GTTCTTGCTT GGGGCTCCAA ACCCCAAGCA GTGGGTGCCC
    CTGGAGGTGC TCCTGGAGGC TTCTCCAGAC AACGCCACCA CTCAACAGAA GCGGGACTTT
    CAGTCTGAGA TCTTGCTTTC CACCATGGAA ATATTTCACG TGATGAGTGG AGGTCATGCA
    TCGATGCTGA GAGGCAGTAG AGAGCCTCAG CCGGATGCAG AAGCTGCTGC TGCTCCTTCC
    CTTGCCAACA TCTCCCACTT CATCCAGAAG CTGGTGGAGA AGCTGTACAG TGGAATGTTC
    TCGGCAGACC CCAGACACAT CCTTCTCTTC ATCACGGAGC ACATCATGGT GGTCATCGAG
    AATGCCTCTG CTCAGAAGGA CACTGCCATC AGTGCACTGT ACAGCAGTCT AAACAAAGTC
    ATCCTTTACA GCCTCTCCAA GCCCCACCAG TCCCTCTCCG AATGCCTCAG CCTCCTCAGC
    ACCCTGGGCT TCCTTCAGGA GCACTGGGAC ATTCTGTTTG CCACCTATAA TTCCAACGCC
    AGCTTCCTCA TGTGTCTCAT GCATTGTCTG CTGCTGCTCA GCGCAAGAAG TTATCCAGAA
    GGATTTGGAT TGGAACCCAA GCCTAGAATG ACTCCTTATC ATCAAGTCTT CCTTTCTCCA
    AATGAAGAAG TGAGAGAAAA AAGAGGGGAA GAATTCCCAA GCTTGAGCGA TGTTCAGCAC
    AATATCCAGA AGACTGTACG GATTCTCTGG CAGCAGCTCG TGGCACAGAG GCGGCAGGAA
    CTGGAGGATA CCTTCAAAAT CGATCTTTCT GTGAAACCTG GAGAGAGTGA AGTGAAGATT
    GAGGAGATCA CCCCACTCTG GGAAGAGACA ATGCTCAAGG CCTGGCAGCA TTATCTAGCA
    TCTGAAAGGA AGTCACTGGC CAGTCGCTCA AACGTTGGAC ACCACAGCAA AGTTTCTTCA
    TGGAGTGGCA GCTTGTCCTC AGCCATGAGG CTGATGCCTG GGCGGCAGAC CAAGGACCCT
    GAGTGCAAAG CAGAGGACTT TGTGTCGTGT ATTGAGAACT ACAGACGAAG AGGACAAGAA
    CTATATGCGT CTCTATACAA AGATCACGTA CAGAGACGGA GATGTGGCAA CATCAAGGCG
    GCCAATGCGT GGACCAGAAT CCAGGAGCAG CTTTTTGGGG AGCTGGGCTT GTGGCATCAG
    ATGGCAGAAG CCACACCCTG CTCCCAGTGG GAACTGGATT GGAGAGAAGG ACCGGCTCGT
    ATGCGGAAAC GCATCAGACG CTTATCTCCA CTGGAGGCCC CTAATCAAGA AAGGCTCAAG
    GAAAGCCAAG ACAGAAATGG CAATGTTTCT CAAATAAACA CTGAACACCA AGACGAGCTG
    ACACTGAAGG AAGGCGAGAG TGAGGCAGAC GAGGTGGCGG GCTGCACCCA GCTGACTTTC
    TTCCCTGCCT TGCACGAAAG CCTGCACTCC GAAGATTTCT TAGAGCTATG TCAGGAAAGA
    CAAGTCATCT TACAAGAGCT TCTTGATCAT GAAAAGGTGA CGCAGAAGTA CTCTGTGGTC
    ATCGTGCAGG GCCACCTGGT CTGCGAAGGG CTCCTGCTCT TCGGCCAGCA GCACTTGTAC
    ATCTGCGAGA ACTTCACGCT GTCTCCCGTG GGTGATGTCT ACTGCACCCG CCACTGCCTA
    TCCAACATTA GCGACCCATT CATCTTCAAC ATGTGCAGCA AAGACAGGTC CTCTGACCAT
    TACTCGTGCC AGCGCCACAG CTACGGCGAC CTCAGGGAGC TCCGGCAGGC CCGCTTCCTC
    CTGCAGGACA TTGCCCTGGA GGTCTTTTTC CAAAATGGAT ATTCCAAGTT TCTTGTCTTC
    CACAACAGTG ATCGGAGTAA GGTATTCAAA AGCTTCTGCT CTTTCCAACC CAGCTTGAAA
    GGAAAAGGCT TCACAGAGGA CACGCTCAAT CTAAGGAGGT ACCCCAGCTC CGACAGGACC
    ATGCTGCAGA AGTGGCAGAA AAGAGACATC AGCAACTTCG AGTACCTCAT GTACCTCAAC
    ACCCTGGCTG GGAGGACCTA CAATGACTAC ATGCAGTATC CAGTGTTCCC CTGGGTCCTG
    GCCGACTACA CCTCACAGAC ACTGAACCTG ATGAATCCCA AGACTTTCCG CGATCTTTCT
    AAGCCCATGG GCGCTCAGAC CAAGGAAAGG AAGCTGAAAT TCATCCAGAG GTTTAAAGAG
    GTTGAGAAGA CTGAAGGAGA CGTGACCGCC CGGTGCCACT ACTGCACCCA CTACTCCTCG
    GCCATCATTG TCGCCTCCTA CCTGGTCCGG ATGCCACCCT TCACCCAGGC CTTCTGCTCT
    CTGCAGGGCG GAAGCTTCGA TGTGGCAGAT AGAATGTTCC ACAGTGTGAA GAATGCGTGG
    GAATCGGCCT CCAGAGAGAA CATGAGTGAT GTCAGAGAGC TGACCCCGGA GTTCTTCTAC
    CTGCCTGAGT TCCTGACCAA CTGCAACGCC CTGGAGTTTG GCTGCATGCA GGACGGAACA
    GCACTGGGCG ATGTTCAGCT CCCTCCCTGG GCTGATGGAG ACCCCCGGAA ATTCATCAGC
    CTGCACAGAC AAGCTTTGGA AAGTGATTTT GTCAGCGCCA ACCTGCACCA CTGGATAGAC
    CTCATTTTTG GGTACAAGCA GCAGGGGTCA GCCGCAGTGG AGGCTGTTAA CACCTTCCAT
    CCCTACTTCT ATGGCGACAA AGTGGACCTC AGCAGCATCA GCGACCCCCT GATCAGAAGC
    ACCATCCTGG GGTTCGTCAG CAACTTTGGA CAGGTGCCCA AACAGCTCTT TACTAAACCC
    CATCCGGCCC GGACTGCCGC AGGGAAGCCT TCCCCTGGCA AGGACGTCTC CACGCCTGCC
    AGCCTGCCCG GGCACCCGCA GCCCTTCTTG TACAGCCTGC AGTCGCTGAG GCCCTCCCAG
    GTCACCGTCA AAGGCTCGGA ATCCCCCAAA GGAGCCATCG GCCACATCGT TCCTACGGAG
    AAGACCATCC TGGCCGTGGA GACAAACAAG ATGCTGCTGC CCCCACACTG GAGCAGGACC
    TTCAGCTGGG GCTTTGACGA CTTCAGCTGC TGCCTGGGCA GCTACGGCTC TGACAAGATC
    TTGGTGACGT TTGAGAACTT GGCCGCCTGG GGCCGCTGTC TGTGTGCTGC ATGCCCGTCC
    CCCACGACCA TCATCACCTC CGGGACCAGC GCCGTGGTGT GTGTTTGGGA GCTCAGCATG
    ACCAAAGGCC GCGCCACAGG CCTGCGCCTC AAGCAGGCCT TGTACGGACA CACGCAGGCT
    GTCACGTGCC TGGCAGCATC AGTCACCTTC AGCCTCCTGG TGAGTGGCTC GCAAGACCGC
    ACGTGCATCC TGTGGGACCT GGACCACCTC ACCCACGTAG CCCGCCTGCC CGCCCACAGG
    GACGGCATAT CTGCTGTTGC CATCAGCGAC GTCTCGGGCA CCATCGTCTC CTGTGCGGGA
    GCCCACTTGT CCCTGTGGGA TGTCAACGGA CAGCCACTGG CCAGCATTAC CACGGCCTGG
    GGCCCAGAAG GAGCCATCAC CTGCTGCTAC GTGGTGGAGG GGCCAGCGTG GGACACAAGC
    CATGTCATCA TCACTGGGAG TCGGGATGGC ATGGTCCGGA TCTGGAAGAC TGAAGATGTG
    AAGATGTCCG TTCCCGGGCA GGCAGCACCG GAGGAGCCAT GGGCTCCATC ATCTCCAAGC
    CCCAGAGTTC ACCGGTGGGA GAAGAATCTT GCCCTTTGCA GAGAGCTGGA CGTCAGCGTG
    GCTTTGACAG GGAAACCCAG CAAGGCCAAC TCTGCAGTGA CAGCTCTGGC CGTGTCCAGA
    AACCAGACCA AACTCCTGGT CGGCGATGAG AAGGGAAGAA TATTCTGCTG GTCTGCAGAG
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.