FREM2 cDNA ORF clone, Bos taurus(cattle)

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221255 XM_025000056.1
Latest version!
Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa221254 XM_002691799.5
Latest version!
Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa24729 XM_015465639.1
Latest version!
Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa24729 XM_002691799.4
Latest version!
Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221255
    Clone ID Related Accession (Same CDS sequence) XM_025000056.1
    Accession Version XM_025000056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GATACTCCGG TTTTTACAAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855824.1
    CDS305..7978
    Translation

    Target ORF information:

    RefSeq Version XM_025000056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GATACTCCGG TTTTTACAAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221254
    Clone ID Related Accession (Same CDS sequence) XM_002691799.5
    Accession Version XM_002691799.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691799.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691845.2
    CDS1..9519
    Translation

    Target ORF information:

    RefSeq Version XM_002691799.5
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691799.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CAATTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24729
    Clone ID Related Accession (Same CDS sequence) XM_002691799.4 , XM_015465639.1
    Accession Version XM_002691799.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691799.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CATTTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691845.1
    CDS1..9519
    Translation

    Target ORF information:

    RefSeq Version XM_002691799.4
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691799.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CATTTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24729
    Clone ID Related Accession (Same CDS sequence) XM_002691799.4 , XM_015465639.1
    Accession Version XM_015465639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
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    3301
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    3421
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    3721
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    4141
    4201
    4261
    4321
    4381
    4441
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    4681
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    5881
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    6181
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CATTTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321125.1
    CDS1..9519
    Translation

    Target ORF information:

    RefSeq Version XM_015465639.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCAAGCCA CCGGGACCCC CGGGGTCACC GCGCGCACGC GCCCCGCAGG AAACCCCACC 
    AAATCTCCAC CGACGCCTCC GACGCCGTCC GGGCTGGTGC TGCTGCTTCT CCTGTCACTG
    GTAAGCCGCG TCCGGGCACA GCCACCTGAC CCCGGCCGGG CCTTGCTGTC CCCGGATCTC
    GCGGGGGTTG CAGGGGTCCC CGCCGAAGAA GCCATAGTGC TGGCGAACCG CGGGCTCCGG
    GTGCCCTTCG GCCGTGAGGT CTGGCTGGAT CCGTTGCAGG ACCTAGTGCT GCAGGTGCAA
    CCGGGGGATC GGTGCGCAGT GACCGTGCTA GACAATGACG CGCTGGCCCA GCGGCCCGGC
    CGCCTGAGTC CCAAGCGCTT CCCCTGCGAC TTCGGCGCGG GAGAGGTGCG CTATTCGCAC
    CTGGGCGCGC GCAGCCCGTC GCGGGATCGA GTCCGGCTGC AGCTGCGCTA CGACGCGCCG
    GGTGGGGCGG CCGTGCTCCC CCTGGTGCTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG
    GTTGTGACGC GAAACCTGCC CTTGGTCGTG GAGGAGCTGC TGGGGACCAG CAATGCCCTG
    GACGCGCGGA GCCTGGAATT CGCCTACCAG CCTGAGACCC AGGAGTGTCG CGTGGGCATC
    CTGCCTGGTT TGGGCGCGCT GCCTCGCTAC GGGGAACTCC TTCACTACCC GCAGGTCCCG
    GGCTCGGCCA GCGAGGGGGA CAGCCAGGAG CCCCTCCTGA TGGACTGTAC AGCATTCCAG
    GAACTGGGCG TGCGCTACCG CCACACCGCC CCAAGTCGTT CTCCGAACAG GGACTGGGTG
    CCCATGGTAG TGGAGCTGCG ATCGCGAGGG TCTCCTGTGG GCAGCCCGGC TTTGAAACGC
    GAGCACTTCC AAGTGCTGGT GAGAATCCGA GGAGGGGCTG AGAACACCGC CCCCAAGCCC
    AGCTTTGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TCCTGACCGC CCTGACCCCA
    GACATGCTGG CAGCCGAGGA TGCGGAGTGT GCCCCAGACT TGCTGATCTT CAATCTCACC
    TCTCCATTCC AGCCCGGCCA GGGCTTCCTG GTGAGCACTG ACGACCGCAG TTTGCCCCTC
    TCCTCCTTCA CTCAGAGGGA TTTGCACCTC CTGAAAATTG CCTATCAGCC CCCCTCTGAA
    GACTCTGACC AGGAGCGTCT CTTTGAACTC GAGCTGGAAG TAGTGGACCC AGAAGGCGCT
    GCCTCTGATC CTTTTTCCTT CATGGTAGTG GTGAAGCCCA TGAACACACT GGCTCCAGTG
    GTCACCCGGA ACACTGGTCT CATTCTCTAC GAGGGCCAGT CTCGGCCCCT CACTGGCCCT
    GCAGGCAGTG GACCACAAAA TTTGGTCATC AGTGATGAAG ACGACTTAGA GGCCGTACAG
    CTGGAGGTGG TGGCCGGGCT CCGGCACGGT CACCTCGTCC TTCTGGGTGC CTCCAATGGC
    AACTCTGCCC CTAAGACCTT TACTGTAGCG GAGCTAGCAG CCGGCCGAGT GGTCTACCAT
    CACGATGACA AAGATGGCTC ACTAAGTGAC AACCTCGTGC TCCTAATGGT GGACGGAGGA
    CGCAGGCACC AGGTGCAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA
    CCAGTCCTCA ATGCCAACAC AGGGCTGACG CTGGCAGAGG GCGAAACCGT GCCCATCCTG
    CCCCAAGCTC TGAGTGCAAT TGACATGGAC TCAGAGGACT CTTTGCTGCT TTTTGTGCTC
    GAGTCACCTT CCTCCACAGC AGGGCATCTG CTTCTCCGCC AGACCCATCC TCCCCAAGGG
    GAGGAGATGC TTCTTGGGAG CCCTTGGAGA AAACAGGGAA TATTCTATGA GAAAACAGTG
    ACACAGTGGC GGCAGCGAGA CATAACAGAG GGGAGACTTT TCTACAGGCA CTCTGGGCCC
    CACAGTCCTG GGCCAGTGAT TGACCAGTTC ACATTTCGTG TCCAGGATAA CCATGACCCC
    CCTAATAAGT CTGGGCTACA GAGGTTCATG ATACGCATTC ATCCTGTGGA CCGCCTCCCT
    CCCGAGCTGG GCAGCGGCTG TCCCCTTCGG ATGGTGGTAC AGGAATCCCA GCTCACACCA
    CTGAGGAAGA AGTGGCTACG CTACACGGAC CTGGACACGG ATGACCGAGA ACTCCGTTAC
    ACGGTGACCC AGCCCCCAAT GGACACAGAT GAAAACCACT CACCAGCCCC GTTGGGCACC
    TTGGTCCTGA CCGATGATCC TTCGGTGGTT GTGGCCCATT TTACACAAGC GCAGGTCAAC
    CACCATAAAA TTGCTTACAG GCCTCCAGAT CAGGAGCTGG GAGTGGCTGC CCGAGTGGCC
    CAGTTTCAAT TCCAGGTGGA GGACCGAGCT GGGAACGTGG CTCCAGGCAC CTTCACCTTT
    TACTTGCAGC CAGTGGACAA CCAGCCACCC GAAATCCTCA ACACTGGCTT CACCGTTCAG
    GAGAAGGGCC ATCACATCCT GAGTGAGACT GAGTTGGGTG TGAATGATGT GGACACTGAT
    GTGGCCCACA TCACCTTCAC TCTCATACAG GTGCCCAAAC ATGGCCACAT GAAGCTGTCT
    GGACAGGTCT TGCATGTCGG TGGGATTTTC CACTTGGAGG ACATAAAACA GGGTCGGATT
    TCCTACGCCC ATAATGGGGC TGAGTCTCTG ACCGATAGCT GTTCCTTGGA GGTCAGTGAC
    AGACACCACG TGGTACCCAT CACTCTCAGA GTGAATGTCC GGCCAATGGA CGGTGAGCTG
    CCCATGCTGA GCCTTCCTGC AGGCACTCTG GGGTCCTATC TAGACGTTTT AGAAAACAGG
    GCTACTGAAA TCACGGCCAA TGTTATCAGG GGGACCAATG GGGACGCTGA TGATTTGATG
    TTGACTTTCC TCTTGGAAGA TCCACCCCTA TATGGAGAAA TCCTGGTCAA TGGAGTTCCA
    GCAGAGCAGT TTACCCAAAG AGACATCTTG GAGGGCTCCG TCGTCTACAC TCACACAAGC
    GGGGAGATAG GTCTCTTGCC CAAAGAAGAC TCTTTCAACC TGAGTCTGTC GGATATGTCT
    CAAGAATGGA GAATAGGTGG TAACACTATC CAAGGAGTGA CCATGTGGGT GACCATCCTC
    CCTGTTGACA GCCAGGCCCC AGAAATCTCC GTAGGTGAGC AGTTTATCGT TCTGGAAGGC
    GATAAAAGTG CTATAACCTC AATGCACATA AGTGCTGAAG ACATGGATTC TCTCAATGAT
    GACATCTTGT GCACCATCGT CATTCAGCCC ACCTCGGGTT ACGTGGAGAA CATCTCTCCA
    GCCCCTGGCT CTGAAAAATC GAGAGCAGGA ATTGCTGTAA GTGCTTTCAC TCTGAAAGAT
    CTCAGGCAAG GTCATATAAA CTACGTCCAG AGTGTCCATA AAGGAGTAGA ACCGGTGGAA
    GACAGATTTA TATTTCGCTG TTCTGATGGC ATTAACTTTT CAGAGAGACA TTTTTTCCCC
    ATTGTAATCA TTCCCACCAA CGATGAACAG CCAGAAATAT TCATCAGGGA ATTTTTGGTG
    ATGGAAGGCA TGAGTTTGGT GGTTGATACA CCCATCCTCA ATGCCGCTGA TGCTGATGTT
    CCCCCGGATG ACTTAACTTT CACTATCACC AAGTTTCCCA CCCACGGTCA CATCATGAAC
    CAGCTGATAA ATGGCACTGT TTTGGTGGAA AGCTTCACCT TGGACCAGAT CATAGAGAGT
    TCCAGCATCA TTTATGAGCA CGATGACTCT GAGACTCAGG AAGACAGTTT CGTGATTCAA
    CTCACAGATG GTAAGCACTC TGTGGAAAAG ACGGTCCTCA TCATGGTTAT TCCTGTTGAT
    GACGAGACCC CTAGGATGGC CATCAATAAT GGACTGGAAA TAGAAATTGG GGAAACCAAA
    ATTATCAACA ACAGAATATT AATGGCAACT GATTTAGATT CTGAAGACAA ATTCTTGGTT
    TACATTATTC GTTATGGGCC GGGACATGGC TTATTACAGA GACAAACACC TACAGGTGTC
    TTTGAAAATA TCACATTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG CAATTTGATT
    CAGTATGTCC ATTTTGGGCA AGAGGGCATT CGGGATCTAA TTAAATTTGA CGTGACTGAT
    GGAATCAATG CCCTCATAGA TCGCTACTTT TATGTGTCTA TTGGAAGCCC TGACATTGTC
    TTCCCTGATG TGATAAGTAA AGGAGTGTCC TTGAAAGAAG GTGGTAAGGT CACCCTCACA
    ACAGATCTAC TGAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTAGT TTTTACCATC
    ACCAGGGCTC CCATGAGAGG CCACTTAGAA AGCACAGACC AGCCTCGAGT GTCTATCACA
    TCTTTCACTC AGCTCCAACT GGCTGGCAAT AAAATCTACT ATATCCACAC AGCTGATGAC
    GAGGTAAAGA TGGACAGTTT TGAGTTTCAG GTCACTGATG GACGTAACCC TGTCTTCCGG
    ACATTCCGTA TCTCCATCAG CGATGTGGAC AACAAAAAGC CAGTGGTCAC CATCCATAAC
    CTGGTTGTCA GCGAAGGTGA AAAGAAGCTG ATTACTCCCT TTGAACTCAC TGTGGAAGAC
    AGGGATACTC CAGACAAACT CTTGAAATTC ACTGTCACCC AAGTGCCTGT TCATGGTCAA
    CTCCTATTCA ACAATACCAG ACCCGCCATG ATTTTTACCA AGCAGGACTT GAATGAAAAC
    TTAATCAGCT ACAGACATGA TGGTACCGAG TCAAGTGAAG ACAGCTTCTC CTTCACAGTG
    ACTGATGGTT CCCATACAGA TTTCTATGTT TTTCCTGATA CAGTATTTGA AACAAGAAGA
    CCCCAAGTAA TGAAGATCCG GATCTTAGCT GTTGACAACA GAGTCCCCCA AATTGTCGTG
    AACAAAGGGG CCTCTACACT TCGCACTCTG GCCACAGGCC ACTTGGGGTT CATGATCACA
    AGTAAAGTAT TGAAAGCAGA GGACAGAGAC AGCTTACACG TTTCTCTCCG GTTTATTGTG
    ACGGAGGCCC CTCGACATGG ATATCTCCTC AACCTGGGCC AGGGCAACCA CAGTGTGACT
    GAGTTTACAC AAGCTGACAT TGATGATATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT
    AATGCCGTAA GTGACATATT CCAGTTTACA GTTGAAGATG GGGGTGGAAA CAAATTAACC
    AAGCAGCATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACTTGATC
    AATGAGGACT CCAAATTTCT AGATGTCATG CTTAAGCGTA GAGGTTATTT GGGAGAGACT
    TCCTTTATAA GTATTGGCAC AAGAGACGGG ACTGCAAAAA AAGACAAGGA CTTCAAGGGC
    AAAGCACAGA GGCAAGTGCA GTTCAACCCA GGCCAGACCC GGGCCACGTG GCGAGTGCGG
    ATTCTGAGCG ACGGGGCGCA CGAGCAGTCC GAGACCTTCC AGGTGGTTCT CGCAGAGCCC
    GTGCTGGCTG CCCTGGAGTT CCCCGCGGTG ACCACAGTGG AGATCGTGGA CCCAGGAGAT
    GAATCAACTG TGTTCATCCC CCAGTCTGAA TACTCCATTG AAGAGGATGT TGGCGAACTG
    TTCATTCCCA TCAGGAGAAG TGGAGACGTG AGCCAGGAGT TGATGGTGGT CTGTTATACG
    CGGCAAGGAA CGGCAAGAGG AACCGTGCCA ACGTCTGTGC TCTCCTATTC TGATTACATA
    TCCCGGCCCG AGGACCACAC CAGTGTTATC CGCTTTGACA AAGATGAGCG GGAGAAGATG
    TGCAGGGTCG TTATAATTGA TGACTCCTTG TTTGAAGAGG AGGAAACATT CCACGTTCTC
    CTGAGTATGC CGATGGGTGG GCGAATTGGA TCAGAGTTCC CAGGGGCTCA GGTTACAATC
    AAACCCGACA AAGACGACGA ACCTGTCTTC TACTTTGGTG ACGTCGAGTA CAAGGTGGAT
    GAGAGTGCGG GCTCGGTGGA GGTGCGGGTG TGGAGGAGTG GCACTGACCT GTCCCAGGCT
    TCTAGTGTCA CCGTGACATC GCAGAAAACA GACCCTCCCT CAGCCGATGC TGGAACAGAC
    TACGTGGGAA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA AACCATTCGT
    GTTATCATTT TGGATGATCT CGGACAACCA ATGCTGGAGG GTATTGAGCA ATTTGAATTG
    GTGCTCCGAA TGCCTATGAA TGCAGCCCTT GGGGACCCCA GCAAAGCCAC GGTGACCATA
    AATGATTCTG TCTCAGATTT ACCTAAGATG CATTTCAAAG AACGAGTATA CACTGGCAAT
    GAAAATGATG GGCAGATAGT CGCCACGATC CACCGGAGTG GGGATACCCG GTACAGGTCT
    TCAGTGAGAT GCTACACGCG ACAGGGCTCT GCACAGGTGA TGATGGACTT CGAAGAACGC
    CCCAATACTG ACATCTCCAT CATCACATTC CTCCCTGGGG AGATCGAAAA GCCTTGCATC
    CTGGAGCTGA TGGATGACGT ATTGTACGAG GAGGTAGAGG AGCTCCGACT GGTTCTTGGC
    ACCCCACAAA GCAACTCTCC TTTTGGAGCT GCAGTTGGGG AACAAAATGA AACTCTGATA
    AGGATCCGAG ACGATGCTGA TAAGACAATT ATTAAATTTG GAGAGACCAA ATTCAGCGTC
    AGTGAACCCA AAGAACCAGG ACAGTCAGTG GTTATAAAAA TTCCAGTGAT TCGCCAAGGA
    GACACTTCAA AAGTTTCCAT CGTGAGAGTT CACACTAAGG ATGGCTCGGC CACTTCTGGA
    GAGGACTATC ACCCTGTGTC GGAAGAAATT GAGTTTAAGG AGGGAGAAAC CCAGCACATT
    GTTGAAATTG AAGTAATATT CGATGGCGTA AGAGAAATGA GAGAGGCCTT CACTGTCCAC
    CTAAAACCTG ATGAAAATAT GATAGCAGAG ACACAGGCGA CCAAAGCCAT CGTGTATATA
    GAAGAAATGA ACAGCATGGC CGATGTCACT TTTCCTTCTG TCCCTCAAGT TGTGTCCTTG
    CTGACGTACG ATGACTCTTC CAGGGCCAAA GAGAGTGCTG GGCCTGTGTC TGGTTATCCT
    GTCGTCTGTA TCACAGCTTG CAACCCTAAA TATCCAGACT ATGAAAAAAC AGGCTCTATT
    TGTGCAAGTG AGAACATCAA TGACACTTCG ACCCGGTACC GATGGCTGAT CAGCGCCCCC
    GCGGGCCCCG ACGGCGTGAC CAGCCCCATG AGAGAAGTGG ACTTTGACAC CTTTTTCACT
    TCGTCCAAGA TGATCACTTT AGACTCCATA TACTTCCAGC CTGGATCCAG AGTGCAGTGT
    GCAGCTCGAG CTGTGAATGC TGACGGCAGT GAAGGCTTGG AGCTGATGAG TCCTATTGTG
    ACCATCGGCA GAGAAGAAGG CCTTTGCCAG CCCCGAGTGC CAGGGATAGT GGGAGCAGAG
    CCCTTCTCAG CTAAACTGCG CTACACGGGC CCCGAGGACT CAGAGTACAC GAACCTCATC
    AAGCTCACCG TCACGATGCC CCACACAGAC GGCATGCTCC CTGTAATCTC TACCAAAGAG
    CTTTCCAACT TTGAGCTCAC ACTCAGCCCT GATGGTACCC GTGTTGGAAA CCACAAGTGC
    TCCAACCTCT TGGATTACAC CGAAGTAAAG ACCCACCACG GTTTCTTGAC TGATGCTACC
    AAAAATCCAG AAATAATTGG AGAGACTTAT CCTTACCAGT ACAGCTCATC TATCAGAGGC
    TCCAATACCT TACGCTTCTA CCGAAACCTG AACCTAGAGG CCTGCTTATG GGAGTTTGTT
    AGCTACTACG ACATGTCCGA GCTGCTCACT GACTGTGGAG GGACCATTGG AACCGATGGA
    CAGGTCCTAA ACCTCGTGCA GTCCTACGTG ACTCTCCGAG TCCCTCTGTA CGTCTCCTAT
    GTGTTCCACT CCCCCGCTGG GGTCGGAGGC TGGCAGCATT TTGACTTGAA GTCCGAACTC
    CGTCTGACAT TCGTGTATGA CACTGCCATC TTGTGGACCG ATGGAATTGG CAGCCCCCCA
    GAAGCTGAAC TCCAAGGTTC TCTCTACCCA ACCAGCATGC GCATCGGTGA AGAGGGGCGT
    TTGGTCGTGA ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TGTTTGTGCT GTCACATCCT
    GCATCCTTCA CAAGCTCAAT GATCATGTCA GTCGATCATC CAGGTCTAAC GTTTTCCCTG
    CGTCTCATAA GAAGTGAACC AACCTATAAC CAGCCAGTCC AGCAGTGGAG CTTCGTATCT
    GACTTTGCAG TCCGTGACTA CTCGGGGACC TACACAGTAA AACTGCTGCC ATGCACCACC
    CCATCCCATC AGGAATTCCG CCTGCCTGTC ACCTGCAACC CCAGAGAGCC TATCACTTTT
    GACCTTGACA TCCGCTTCCA ACAGGTCAGT GACCCAGTGG CGGCTGAGTT TAGCTTGAAC
    ACCCAGATGT ACCTCCTCTC CAAGAAGAGC CTCTGGCTGT CTGACGGATC CATGGGATTT
    GGGCAGGAGA GCGACGTTGC CTTTGCAGAA GGTGATATCA TTTATGGTCG GGTCATGGTG
    GATCCAGTCC AGAATCTAGG TGATTCCTTT TACTGCAGCA TTGAAAAGGT GTTTCTGTGC
    ACCGGGGCTG ACGGCTACGT TCCCAAGTAC AATCCAACGA ACGCAGAATA CGGCTGCTTG
    GCTGATTCAC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA ACCAGAGACA
    CAAGCCACCA GCTTTGGAAA TGTCCTGTTT AATGCCAAAC TGGCAGTGGA TGACCCTGAA
    GCTATCCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC
    TTTCAGGTTG CTCTGGGTCG AGAATGGTAC GTACACACCA TCTACACAGT AAGATCGAAA
    GACAGTGCCA ACCGAGGCAT TGGCAAACGC AGCGTGGAGT ACCGGTACCA CTCTTTGGTG
    GGTCAAGGGA AGCCCCAGGC AGCTACCAAG AGCCGGAAGA AGAGGGAGAT CAGAAGCAGC
    CCTCCGCTGG CGTGGGAAAT CGGGGCTGAG AACAATCGGG GAACGAACAT CCAGCACGTC
    GCCCTGGACC GCAGCAGCAG GAAGCAGATC CTTCACGGGA GGGTTTCCCC TGACGGCGTC
    CTCCCCCGGG AGCTCAACAG TCCCAGCTCT GAGGTCAGCC TGCTCACGGT GGTGGGGGGC
    GTCGTGGTGG GACTCCTGGC CGTCATCCTC ACTGCCGTCA CGGTGAGGAT GCACCAGGGC
    AGGAAGGGTG CCAGGAGGAA GGATGCACCC AAGGACCCCG GCAGCCGTGA GCCCATGATG
    CCCTCAGTGA GCCACCACAG CGACAGCTCG GAGGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.