TRPM6 cDNA ORF clone, Bos taurus(cattle)

The following TRPM6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa91258 XM_015472507.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa91258 XM_015464531.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa237433 XM_024996175.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237434 XM_024996176.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237435 XM_015472505.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa91257 XM_015464529.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa91257 XM_015472505.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa51414 XM_010798669.2
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa51414 XM_010807896.2
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa51415 XM_010807897.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa51415 XM_010798670.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa51416 XM_015472506.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa51416 XM_015464530.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa91258
    Clone ID Related Accession (Same CDS sequence) XM_015464531.1 , XM_015472507.1
    Accession Version XM_015464531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAATTTGGC CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA
    ATGAAAGAAT GGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320017.1
    CDS757..2874
    Translation

    Target ORF information:

    RefSeq Version XM_015464531.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAATTTGGC CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA
    ATGAAAGAAT GGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91258
    Clone ID Related Accession (Same CDS sequence) XM_015464531.1 , XM_015472507.1
    Accession Version XM_015472507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAATTTGGC CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA
    ATGAAAGAAT GGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327993.1
    CDS757..2874
    Translation

    Target ORF information:

    RefSeq Version XM_015472507.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAATTTGGC CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA
    ATGAAAGAAT GGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237433
    Clone ID Related Accession (Same CDS sequence) XM_024996175.1
    Accession Version XM_024996175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CCAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGC GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCG GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT TCAGAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851943.1
    CDS182..5152
    Translation

    Target ORF information:

    RefSeq Version XM_024996175.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CCAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGC GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCG GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT TCAGAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237434
    Clone ID Related Accession (Same CDS sequence) XM_024996176.1
    Accession Version XM_024996176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCCAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGCGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCGGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATTCAGA AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851944.1
    CDS521..5476
    Translation

    Target ORF information:

    RefSeq Version XM_024996176.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCCAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGCGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCGGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATTCAGA AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237435
    Clone ID Related Accession (Same CDS sequence) XM_015472505.2
    Accession Version XM_015472505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CCAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCG TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CAACCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GATTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAT CTCACACGAT TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327991.2
    CDS177..6281
    Translation

    Target ORF information:

    RefSeq Version XM_015472505.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CCAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCG TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CAACCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GATTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAT CTCACACGAT TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91257
    Clone ID Related Accession (Same CDS sequence) XM_015472505.1 , XM_015464529.1
    Accession Version XM_015472505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GACTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAC CTCACACGAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327991.1
    CDS748..6852
    Translation

    Target ORF information:

    RefSeq Version XM_015472505.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GACTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAC CTCACACGAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91257
    Clone ID Related Accession (Same CDS sequence) XM_015472505.1 , XM_015464529.1
    Accession Version XM_015464529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GACTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAC CTCACACGAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320015.1
    CDS748..6852
    Translation

    Target ORF information:

    RefSeq Version XM_015464529.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GACTTAAAAA GAAATGACTA CTCTCCTGGA
    AGGATAAATT CTGCCTTTGG ACTTGAAATC AAAATAGAAC CAGCTGAGGA GACTCCAGCG
    GGGGAAGAGG GTAGTAATTC TCCAGAAGAC CTCACACGAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51414
    Clone ID Related Accession (Same CDS sequence) XM_010807896.2 , XM_010798669.2
    Accession Version XM_010807896.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCTAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGGGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCTGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATATCAG AAAATGAAAA CTAAAGGAGC TGAACGACAT
    CCTGTACAAG TCAGTGATTA TGGAAGGCAG TCTCAAGAGG ATCTTAGCAA AAGCTCTTTG
    TGGGATTCCA GGAACACCAC AGTCAATAGG AAGTCTCAGT TGAGAAGTTC AAATGGAGTT
    AACAAAATCT CAGACTCCTT AAAAAGCCCT CAAGAGCCTC ACCATCATTA TTCAGCCATT
    GAAAGGAACA ATTTAATGAG GCTTTCTCAG ACCATACCGT TTACACCAAT CCAACTGTTT
    GCAGGAGAAG AAGTAACAGT CTACAGGCTG GAGGAGAGTT CTCCCTTGAA TCTGGATAAA
    AGCATGTCTT CTTGGTCCCA GCGGGGGAGA TCTGCTATGA TCCAGGTGTT GTCCCAAGAG
    GAGATGGATG GTGGCCTTCG CAAAGCCATG AGAGTCATTA GCACCTGGTC TGAGGATGAC
    ATCCTCAAGC CAGGGCAGGT TTTCATTGTG AAGTCCTTTC TTCCTGAGGT TGTGCAGACA
    TGGCACGAAA TCTTCCAGGA GAGTACTGTG CTTCATCTTT GCCTTCGGGA AATTCAACAA
    CAAAGAGCTG CTCAAAAACT GATCTATACC TTCAACCAAG TAAAACCACA AACCATTCCC
    TACACTCCAA GGTTCCTGGA AGTTTTCTTA ATTTACTGCC ATTCAGCCCA GCAGTGGTTG
    ACCATCGAGA AGTATATGAC GGGGGAGTTC CGGAAGTACA ACAACAACAA CGGTGATGAA
    ATTGCCCCCA GCAACACCTT GGAGGAGCTG ATGTTGGCTT TCTCTCACTG GACCTATGAG
    TATACTCGGG GAGAGCTGCT GGTTTTAGAT TTGCAAGGTG TTGGAGAAAA TTTGACGGAT
    CCATCTGTAA TAAAACCTGA AGACAAACAA TCAAGAGGAA TGGTGTTTGG ACCAGCCAAT
    TTAGGGGAAG ATGCAATTAG AAACTTCATT GCAAAACATC GTTGCAACTC CTGCTGCCGG
    AAGCTCAAAC TCCCGGACTT AAAAAGAAAT GACTACTCTC CTGGAAGGAT AAATTCTGCC
    TTTGGACTTG AAATCAAAAT AGAACCAGCT GAGGAGACTC CAGCGGGGGA AGAGGGTAGT
    AATTCTCCAG AAGACCTCAC ACGATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806198.1
    CDS531..6620
    Translation

    Target ORF information:

    RefSeq Version XM_010807896.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807896.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCTAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGGGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCTGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATATCAG AAAATGAAAA CTAAAGGAGC TGAACGACAT
    CCTGTACAAG TCAGTGATTA TGGAAGGCAG TCTCAAGAGG ATCTTAGCAA AAGCTCTTTG
    TGGGATTCCA GGAACACCAC AGTCAATAGG AAGTCTCAGT TGAGAAGTTC AAATGGAGTT
    AACAAAATCT CAGACTCCTT AAAAAGCCCT CAAGAGCCTC ACCATCATTA TTCAGCCATT
    GAAAGGAACA ATTTAATGAG GCTTTCTCAG ACCATACCGT TTACACCAAT CCAACTGTTT
    GCAGGAGAAG AAGTAACAGT CTACAGGCTG GAGGAGAGTT CTCCCTTGAA TCTGGATAAA
    AGCATGTCTT CTTGGTCCCA GCGGGGGAGA TCTGCTATGA TCCAGGTGTT GTCCCAAGAG
    GAGATGGATG GTGGCCTTCG CAAAGCCATG AGAGTCATTA GCACCTGGTC TGAGGATGAC
    ATCCTCAAGC CAGGGCAGGT TTTCATTGTG AAGTCCTTTC TTCCTGAGGT TGTGCAGACA
    TGGCACGAAA TCTTCCAGGA GAGTACTGTG CTTCATCTTT GCCTTCGGGA AATTCAACAA
    CAAAGAGCTG CTCAAAAACT GATCTATACC TTCAACCAAG TAAAACCACA AACCATTCCC
    TACACTCCAA GGTTCCTGGA AGTTTTCTTA ATTTACTGCC ATTCAGCCCA GCAGTGGTTG
    ACCATCGAGA AGTATATGAC GGGGGAGTTC CGGAAGTACA ACAACAACAA CGGTGATGAA
    ATTGCCCCCA GCAACACCTT GGAGGAGCTG ATGTTGGCTT TCTCTCACTG GACCTATGAG
    TATACTCGGG GAGAGCTGCT GGTTTTAGAT TTGCAAGGTG TTGGAGAAAA TTTGACGGAT
    CCATCTGTAA TAAAACCTGA AGACAAACAA TCAAGAGGAA TGGTGTTTGG ACCAGCCAAT
    TTAGGGGAAG ATGCAATTAG AAACTTCATT GCAAAACATC GTTGCAACTC CTGCTGCCGG
    AAGCTCAAAC TCCCGGACTT AAAAAGAAAT GACTACTCTC CTGGAAGGAT AAATTCTGCC
    TTTGGACTTG AAATCAAAAT AGAACCAGCT GAGGAGACTC CAGCGGGGGA AGAGGGTAGT
    AATTCTCCAG AAGACCTCAC ACGATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51414
    Clone ID Related Accession (Same CDS sequence) XM_010807896.2 , XM_010798669.2
    Accession Version XM_010798669.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCTAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGGGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCTGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATATCAG AAAATGAAAA CTAAAGGAGC TGAACGACAT
    CCTGTACAAG TCAGTGATTA TGGAAGGCAG TCTCAAGAGG ATCTTAGCAA AAGCTCTTTG
    TGGGATTCCA GGAACACCAC AGTCAATAGG AAGTCTCAGT TGAGAAGTTC AAATGGAGTT
    AACAAAATCT CAGACTCCTT AAAAAGCCCT CAAGAGCCTC ACCATCATTA TTCAGCCATT
    GAAAGGAACA ATTTAATGAG GCTTTCTCAG ACCATACCGT TTACACCAAT CCAACTGTTT
    GCAGGAGAAG AAGTAACAGT CTACAGGCTG GAGGAGAGTT CTCCCTTGAA TCTGGATAAA
    AGCATGTCTT CTTGGTCCCA GCGGGGGAGA TCTGCTATGA TCCAGGTGTT GTCCCAAGAG
    GAGATGGATG GTGGCCTTCG CAAAGCCATG AGAGTCATTA GCACCTGGTC TGAGGATGAC
    ATCCTCAAGC CAGGGCAGGT TTTCATTGTG AAGTCCTTTC TTCCTGAGGT TGTGCAGACA
    TGGCACGAAA TCTTCCAGGA GAGTACTGTG CTTCATCTTT GCCTTCGGGA AATTCAACAA
    CAAAGAGCTG CTCAAAAACT GATCTATACC TTCAACCAAG TAAAACCACA AACCATTCCC
    TACACTCCAA GGTTCCTGGA AGTTTTCTTA ATTTACTGCC ATTCAGCCCA GCAGTGGTTG
    ACCATCGAGA AGTATATGAC GGGGGAGTTC CGGAAGTACA ACAACAACAA CGGTGATGAA
    ATTGCCCCCA GCAACACCTT GGAGGAGCTG ATGTTGGCTT TCTCTCACTG GACCTATGAG
    TATACTCGGG GAGAGCTGCT GGTTTTAGAT TTGCAAGGTG TTGGAGAAAA TTTGACGGAT
    CCATCTGTAA TAAAACCTGA AGACAAACAA TCAAGAGGAA TGGTGTTTGG ACCAGCCAAT
    TTAGGGGAAG ATGCAATTAG AAACTTCATT GCAAAACATC GTTGCAACTC CTGCTGCCGG
    AAGCTCAAAC TCCCGGACTT AAAAAGAAAT GACTACTCTC CTGGAAGGAT AAATTCTGCC
    TTTGGACTTG AAATCAAAAT AGAACCAGCT GAGGAGACTC CAGCGGGGGA AGAGGGTAGT
    AATTCTCCAG AAGACCTCAC ACGATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796971.1
    CDS531..6620
    Translation

    Target ORF information:

    RefSeq Version XM_010798669.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798669.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGAGTGC AGGTCAAGTC CCAGAAATCT TGGATTGAAG GAGTGTTCAA CAAGAGAGAA 
    TGTAACAAAA TCATACCCAG CTCTAAAGAC CCTCACAGAT GCACTGCAGG ATGTCAGGTC
    TGTCAGAATT TAATCAGGTG TTACTGTGGA CTATTGATTA GAGACCATCA TGGGATAGAT
    TATACTCGGA CTGTCTCAGC TGCCAGCGGT AGTGAAGATG AACAGTGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCTAC AGACACCTTT GGCACGATTA ACTTCCAACA TGGAGAGCAC
    ATCCACCACT CCAAGTATAT TAGAACTTCT TATGACACAA AGTTGGATCA CCTGTTACAT
    TTAATGTTGA AAGAGTGGAA GATGGAACTG CCAAAGCTGG TGATCTCCGT CCATGGTGGT
    ATCCAGAACT TCAAGATGCC CTCAAAACTT AAAGAGATCT TCAGCCAAGG TTTGGTCAAA
    GCTGCAGAGA CAACAGGAGC ATGGATAATA ACTGAAGGCA TTAATACAGG TGTGTCTAAA
    CATGTTGGGG ATGCCCTGAA AGCCCATTCC TCGCAGTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CGGAAAGATC TTATTGGAAG AGATGTAGTG
    TGCCTGTACC AGACTCTGGG AAACCCCCTC AGCAAGCTCA CAACACTCAA CTGCATGCAC
    TCCCACTTTA TCCTGTCTGA TGATGGCACT GTGGGCAAGT ATGGCAATGA GATGAAGCTC
    AGGAGGAACC TGGAAAAGTA CCTCTCTCTG CAGAAGATAC ATAGTTGCTC TGGACAAGGC
    GTGCCCGTGG TGGGGCTGGT GGTGGAAGGT GGTCCCAACG TCATCCTCTC CGTGTGGGAG
    ACTGTGAAAG ACAAAGACCC GGTGGTGGTG TGCGAAGGCA CAGGCAGGGC TGCTGACCTC
    CTGGCCTTCA CCCACAAACA CTTAGCAGAT GAAGGGACCC TGCGTCCTCA GGTGAAGGAG
    GAGATCATCT GCATGATGCA GAACACCTTC AACTTTAGTC TTAAGCAGTC CAAGCACCTT
    TTCCAAATTC TGATGGAGTG TATGGTGCAC AGGGATTCTA TTACCATCTT TGATGCTGAT
    TCTGAGGAGT CACAGGACCT TGATTTAGCA ATCCTAACAG CTCTGCTAAA GGGCACGAAT
    TTATCAGCAT CAGAACAATT GAATCTGGCA ATGGCTTGGG ACAGAATGGA CATTGCCAAG
    AAACATATCC TAATTTATGG ACAACACTGG AAGCCTGGCT CTCTAGAACA AGCGATGTTA
    GATGCCTTGA TGATGGATCG AGTTGATTTT GTGAAGCTCT TAATAGAGTA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CTCCCGACTG GAAGAGCTCT ATAATACAAA ACAAGGACCA
    ACCAATATGC TCTTACATCA TCTTGTCCAA GATGTAAAAC AGCATACACT TCTCTCAGGC
    TATAAGATAA CGCTGATTGA CATCGGATTG GTAATAGAAT ACCTTATTGG TGGAGCATAT
    CGCAGCAGCT ACACTAGAAA AACTTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAACAT
    AAGCAAGTGA CCACTCTTGC TCAAAACCTG TCCCAGCCTG CTCTTCACCG AAGACGCTGC
    TCAGGAAATA GAAAGGAGTC TACAGAGAGC ACCCTGCATT CCCAGTTCAT TAGAACTGCC
    CAACCTTACA AAGTCAAGGA AAAGTCTGCA GCCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAAGAACAAA ATATATCAGA TGACCCTGAT TCTACTGGCT TTATTTATCC TTATAATGAC
    CTGCTGGTGT GGGCTGTGCT AATGAAAAGG CAGAAGATGG CCATGTTCTT CTGGCAGCAT
    GGTGAGGAGG CCACGGTCAA GGCAGTGATC GCTTGTATCC TCTACCGAGC AATGGCCCGG
    GAAGCCAAGG AGAGCAACAT GGTCGATGAT GCCTCTGAAG AACTGAAAAA TTACTCAAAA
    CAGTTTGGCC AGCTTGCCCT GGATGTGTTG GAGAAGGCAT TCAAACAGAA TGAAAGAATG
    GCCATGAAAC TGCTGACCTA CGAACTCAAG AACTGGAGCA ACTCTACCTG TTTGAAGCTG
    GCTGTTTCTG GAGGGTTGAG GCCCTTTGTT TCACACACTT GTACCCAAAT GCTACTAACT
    GATATGTGGA TGGGGCGTCT GAAAATGAGG AAAAACTCTT GGTTAAAGAT CATCATCAGT
    ATTCTTTTCC CACCTGCCAT TTTGACACTG GAGTTTAAAA GCAAAGCAGA AATGTCACAT
    GTCCCACAGT CCCAGGACTT TACGTGGTAT AATGGTGACC AGAACACCAG TACTCTCAAA
    GAAAATTCTT GTCAGAAAGA CTGTGACTTG GAAAGGGGAC CTGATGAGAA AACAGATGAA
    AGCCAGCACT TTGATTTAAA GAGTGGGCTC CAAACCCTTC CTTGGACCAG GAAAGTCTAT
    GAGTTCTACA ATGCTCCAAT TGTCAAGTTT TGGTTTTATA CGATGGTGTA CTTGGCATTC
    CTTATGCTGT TCACTTACAC CGTGTTGGTG GAGATGCAGC CCCAGCCCAG CGTGCAAGAG
    TGGCTTGTTA TTATCTACAT CTTCACCAAT GCCATTGAGA AAGTCAGGGA GGTCTGTGTT
    TCAGAACCTG GGAAGTTTAC TCAAAAGGTG AAGGTGTGGA TTAGCGAGTA CTGGAACTTA
    ATGGAAACTG TGGCTATTGG CCTGTTTTTG GTGGGATTTG GCTTTCGGTG GGGTGCCCCA
    CCTTTCTACA CGGCGGGAAG ACTGATATAC TGCATCAACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCCT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA CATTGTGATC ATCATGGCCA TCGTCCTCCT GAGCTTTGGA
    GTGGCACGCA AAGCCATCCT TTCACCAAAG GAGCCCCCAT CCTGGACCCT TGCCCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAT GGAGAAGTCT ATGCTTCAGA GATAGATGTT
    TGTTCAAGCC AGCCGTCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCCT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT TAATAACGTT
    TACATAGATC TGAAATCCAT TTCAAATAAC CTGTGGAAAT ATAATCGCTA TCGTTACATC
    ATGACCTACC ATGAAAAGCC TTGGTTGCCC CCACCTTTTA TCCTGCTGAG TCACATTGGT
    CTCCTGCTTC GCCGTCTTTG CCAGCACCAA GCTCCAAATG ACCAAGAAGA GGGTGATGTT
    GGATTAAAAC TCTACCTAAG TAAGGAGGAT CTGAAAAAAC TTCACGATTT TGAAGAACAG
    TGTGTGGAAA AGTACTTCCA CGAGAAGATG GAAGATCTGA ATTGTAGTTG TGAGGAACAA
    ATCCGAGTGA CATCAGAAAG GGTTACCGAG ATGTACTTCC AAATGAAAGA AATGAATGAA
    AAGGTGTCTT TCATAAAGGA CTCCTTATTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG TCCTGACAGT GGATACCTTA AAAGTCCTTT CTGCTGTTGA CACCTTACAA
    GAAGATGAGG CTCTCCTGGC CAACAGAAAG CATTCTACTT GCAGAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTG GGCAGCATGC CACTCTCTGA GAAGAAGAAG
    TATCAGTGTT ATAGCATGCC CCCTTCTTTG CTGCGGAGCC TGGCCAGAGG CTGGTATCCC
    CCACGAGGGC AGAAAGGGCC CTTTCTTGAG ATTACAGATC AAGAGGCTTC AAATGTAAGA
    GATGACCAAG GAGAGCAAGA AACAAAAAAT CGTGTAGTTG CTTCTGGGGT GATGCTGAAC
    AGACAGGCAC ACCCAAAGTA TGGCCAATAT CTCCTGGTCC CTTCTTATCT AGAACAAGTT
    TCTTATTCTG CAAAAACTGA CTTGCCTCTG TCCACACCAT CTGTGGAAGC AGGTACAGAT
    GGGCTGACAA CTGAACACGT CAGCCAGACT GAGGTCCCTG TTCATCTGAT GTGGCAGACC
    CCGGGTGTCT CAGCCAAGGG CCCCATGGTG GAAACCAAGG AGCAGTGTGA GTCACTTGGT
    CAGATACCAG CCAGACAAGA CAAGGGGGAA CGAGTGCTCC CCACTTTGAT TTGCACACCT
    GCACCCACGA GAGTGTCCTC CCTCCCTTCC CAGGTCATGA GCATGCACCC TGGAGGTGGG
    TATGTGAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAC CAAGAAGCAA
    TGTCAAATCA GCATGGCCTC CGCTTGTAAC CATGACTCCA GACTGAGTGA ACAGGGCCAG
    AGGCAGAACC GGGGCAGATC CCTGTCTGAT AACTCGGCAA GACTGGCCCA GAGGAATTGC
    TCAGAAGTAC TTCCATGGCT CCAGCCAAAC ACATCCTTTC GAATCAGTCT GCTTCGGAAA
    GACAAGCCCT TCCCTGGGAA TCAGAGCTTG AGATTACCCA AGGAGGAGAA GTTGAAGATC
    TCTAAGATTA AAAATCTATC AAAATTCTCA GAGATAGGAC AATCAAAATG GATCAAAACA
    AAATTGCTAA CCAAGGATAG GAAACTGTCA AAGAAGAAGA AGAAAACACA AGCACTGCAG
    GTGCCAGTCA TAACAGTCAA CACCTGCTCT CAAAGTGACC AGTTGAACCC AGAGCCTGGA
    GAAAAGATCT CAGAAGCACA CAAGGGCAGC AAGAACTGGA TTCCAGTGTC CAAATTTAAT
    CAGATAAGTC TAGAATCTTA CATATATCAG AAAATGAAAA CTAAAGGAGC TGAACGACAT
    CCTGTACAAG TCAGTGATTA TGGAAGGCAG TCTCAAGAGG ATCTTAGCAA AAGCTCTTTG
    TGGGATTCCA GGAACACCAC AGTCAATAGG AAGTCTCAGT TGAGAAGTTC AAATGGAGTT
    AACAAAATCT CAGACTCCTT AAAAAGCCCT CAAGAGCCTC ACCATCATTA TTCAGCCATT
    GAAAGGAACA ATTTAATGAG GCTTTCTCAG ACCATACCGT TTACACCAAT CCAACTGTTT
    GCAGGAGAAG AAGTAACAGT CTACAGGCTG GAGGAGAGTT CTCCCTTGAA TCTGGATAAA
    AGCATGTCTT CTTGGTCCCA GCGGGGGAGA TCTGCTATGA TCCAGGTGTT GTCCCAAGAG
    GAGATGGATG GTGGCCTTCG CAAAGCCATG AGAGTCATTA GCACCTGGTC TGAGGATGAC
    ATCCTCAAGC CAGGGCAGGT TTTCATTGTG AAGTCCTTTC TTCCTGAGGT TGTGCAGACA
    TGGCACGAAA TCTTCCAGGA GAGTACTGTG CTTCATCTTT GCCTTCGGGA AATTCAACAA
    CAAAGAGCTG CTCAAAAACT GATCTATACC TTCAACCAAG TAAAACCACA AACCATTCCC
    TACACTCCAA GGTTCCTGGA AGTTTTCTTA ATTTACTGCC ATTCAGCCCA GCAGTGGTTG
    ACCATCGAGA AGTATATGAC GGGGGAGTTC CGGAAGTACA ACAACAACAA CGGTGATGAA
    ATTGCCCCCA GCAACACCTT GGAGGAGCTG ATGTTGGCTT TCTCTCACTG GACCTATGAG
    TATACTCGGG GAGAGCTGCT GGTTTTAGAT TTGCAAGGTG TTGGAGAAAA TTTGACGGAT
    CCATCTGTAA TAAAACCTGA AGACAAACAA TCAAGAGGAA TGGTGTTTGG ACCAGCCAAT
    TTAGGGGAAG ATGCAATTAG AAACTTCATT GCAAAACATC GTTGCAACTC CTGCTGCCGG
    AAGCTCAAAC TCCCGGACTT AAAAAGAAAT GACTACTCTC CTGGAAGGAT AAATTCTGCC
    TTTGGACTTG AAATCAAAAT AGAACCAGCT GAGGAGACTC CAGCGGGGGA AGAGGGTAGT
    AATTCTCCAG AAGACCTCAC ACGATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51415
    Clone ID Related Accession (Same CDS sequence) XM_010807897.1 , XM_010798670.1
    Accession Version XM_010807897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAGAGGT CCCAGAAATC TTGGATTGAA GGAGTGTTCA ACAAGAGAGA ATGTAACAAA 
    ATCATACCCA GCTCTAAAGA CCCTCACAGA TGCACTGCAG GATGTCAGGT CTGTCAGAAT
    TTAATCAGGT GTTACTGTGG ACTATTGATT AGAGACCATC ATGGGATAGA TTATACTCGG
    ACTGTCTCAG CTGCCAGCGG TAGTGAAGAT GAACAGTGGT CTGTTGAAAA GCACACAACG
    AAAAGCCCTA CAGACACCTT TGGCACGATT AACTTCCAAC ATGGAGAGCA CATCCACCAC
    TCCAAGTATA TTAGAACTTC TTATGACACA AAGTTGGATC ACCTGTTACA TTTAATGTTG
    AAAGAGTGGA AGATGGAACT GCCAAAGCTG GTGATCTCCG TCCATGGTGG TATCCAGAAC
    TTCAAGATGC CCTCAAAACT TAAAGAGATC TTCAGCCAAG GTTTGGTCAA AGCTGCAGAG
    ACAACAGGAG CATGGATAAT AACTGAAGGC ATTAATACAG GTGTGTCTAA ACATGTTGGG
    GATGCCCTGA AAGCCCATTC CTCGCAGTCC TTGAGAAAAA TCTGGACAGT TGGAATCCCT
    CCTTGGGGTG TCATTGAGAA CCGGAAAGAT CTTATTGGAA GAGATGTAGT GTGCCTGTAC
    CAGACTCTGG GAAACCCCCT CAGCAAGCTC ACAACACTCA ACTGCATGCA CTCCCACTTT
    ATCCTGTCTG ATGATGGCAC TGTGGGCAAG TATGGCAATG AGATGAAGCT CAGGAGGAAC
    CTGGAAAAGT ACCTCTCTCT GCAGAAGATA CATAGTTGCT CTGGACAAGG CGTGCCCGTG
    GTGGGGCTGG TGGTGGAAGG TGGTCCCAAC GTCATCCTCT CCGTGTGGGA GACTGTGAAA
    GACAAAGACC CGGTGGTGGT GTGCGAAGGC ACAGGCAGGG CTGCTGACCT CCTGGCCTTC
    ACCCACAAAC ACTTAGCAGA TGAAGGGACC CTGCGTCCTC AGGTGAAGGA GGAGATCATC
    TGCATGATGC AGAACACCTT CAACTTTAGT CTTAAGCAGT CCAAGCACCT TTTCCAAATT
    CTGATGGAGT GTATGGTGCA CAGGGATTCT ATTACCATCT TTGATGCTGA TTCTGAGGAG
    TCACAGGACC TTGATTTAGC AATCCTAACA GCTCTGCTAA AGGGCACGAA TTTATCAGCA
    TCAGAACAAT TGAATCTGGC AATGGCTTGG GACAGAATGG ACATTGCCAA GAAACATATC
    CTAATTTATG GACAACACTG GAAGCCTGGC TCTCTAGAAC AAGCGATGTT AGATGCCTTG
    ATGATGGATC GAGTTGATTT TGTGAAGCTC TTAATAGAGT ATGGAGTGAA CCTCCATCGC
    TTTCTTACCA TCTCCCGACT GGAAGAGCTC TATAATACAA AACAAGGACC AACCAATATG
    CTCTTACATC ATCTTGTCCA AGATGTAAAA CAGCATACAC TTCTCTCAGG CTATAAGATA
    ACGCTGATTG ACATCGGATT GGTAATAGAA TACCTTATTG GTGGAGCATA TCGCAGCAGC
    TACACTAGAA AAACTTTCAG AGCCCTCTAC AACAACCTCT ACAGAAAACA TAAGCAAGTG
    ACCACTCTTG CTCAAAACCT GTCCCAGCCT GCTCTTCACC GAAGACGCTG CTCAGGAAAT
    AGAAAGGAGT CTACAGAGAG CACCCTGCAT TCCCAGTTCA TTAGAACTGC CCAACCTTAC
    AAAGTCAAGG AAAAGTCTGC AGCCCTTCAT AAATCAAGGA AGAAGTCAAA AGAAGAACAA
    AATATATCAG ATGACCCTGA TTCTACTGGC TTTATTTATC CTTATAATGA CCTGCTGGTG
    TGGGCTGTGC TAATGAAAAG GCAGAAGATG GCCATGTTCT TCTGGCAGCA TGGTGAGGAG
    GCCACGGTCA AGGCAGTGAT CGCTTGTATC CTCTACCGAG CAATGGCCCG GGAAGCCAAG
    GAGAGCAACA TGGTCGATGA TGCCTCTGAA GAACTGAAAA ATTACTCAAA ACAGTTTGGC
    CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA ATGAAAGAAT GGCCATGAAA
    CTGCTGACCT ACGAACTCAA GAACTGGAGC AACTCTACCT GTTTGAAGCT GGCTGTTTCT
    GGAGGGTTGA GGCCCTTTGT TTCACACACT TGTACCCAAA TGCTACTAAC TGATATGTGG
    ATGGGGCGTC TGAAAATGAG GAAAAACTCT TGGTTAAAGA TCATCATCAG TATTCTTTTC
    CCACCTGCCA TTTTGACACT GGAGTTTAAA AGCAAAGCAG AAATGTCACA TGTCCCACAG
    TCCCAGGACT TTACGTGGTA TAATGGTGAC CAGAACACCA GTACTCTCAA AGAAAATTCT
    TGTCAGAAAG ACTGTGACTT GGAAAGGGGA CCTGATGAGA AAACAGATGA AAGCCAGCAC
    TTTGATTTAA AGAGTGGGCT CCAAACCCTT CCTTGGACCA GGAAAGTCTA TGAGTTCTAC
    AATGCTCCAA TTGTCAAGTT TTGGTTTTAT ACGATGGTGT ACTTGGCATT CCTTATGCTG
    TTCACTTACA CCGTGTTGGT GGAGATGCAG CCCCAGCCCA GCGTGCAAGA GTGGCTTGTT
    ATTATCTACA TCTTCACCAA TGCCATTGAG AAAGTCAGGG AGGTCTGTGT TTCAGAACCT
    GGGAAGTTTA CTCAAAAGGT GAAGGTGTGG ATTAGCGAGT ACTGGAACTT AATGGAAACT
    GTGGCTATTG GCCTGTTTTT GGTGGGATTT GGCTTTCGGT GGGGTGCCCC ACCTTTCTAC
    ACGGCGGGAA GACTGATATA CTGCATCAAC ATCATATTCT GGTTCTCACG GCTCCTGGAC
    TTCTTTGCTG TGAATCAACA TGCAGGTCCC TATGTGACCA TGATTGCAAA AATGACAGCA
    AACATGTTCT ACATTGTGAT CATCATGGCC ATCGTCCTCC TGAGCTTTGG AGTGGCACGC
    AAAGCCATCC TTTCACCAAA GGAGCCCCCA TCCTGGACCC TTGCCCGAGA TATTGTATTT
    GAGCCATACT GGATGATATA TGGAGAAGTC TATGCTTCAG AGATAGATGT TTGTTCAAGC
    CAGCCGTCCT GCCCTCCTGG TTCTTTTCTT ACTCCATTCC TGCAAGCTGT CTACCTCTTC
    GTGCAATATA TCATCATGGT GAACCTGTTG ATTGCTTTCT TTAATAACGT TTACATAGAT
    CTGAAATCCA TTTCAAATAA CCTGTGGAAA TATAATCGCT ATCGTTACAT CATGACCTAC
    CATGAAAAGC CTTGGTTGCC CCCACCTTTT ATCCTGCTGA GTCACATTGG TCTCCTGCTT
    CGCCGTCTTT GCCAGCACCA AGCTCCAAAT GACCAAGAAG AGGGTGATGT TGGATTAAAA
    CTCTACCTAA GTAAGGAGGA TCTGAAAAAA CTTCACGATT TTGAAGAACA GTGTGTGGAA
    AAGTACTTCC ACGAGAAGAT GGAAGATCTG AATTGTAGTT GTGAGGAACA AATCCGAGTG
    ACATCAGAAA GGGTTACCGA GATGTACTTC CAAATGAAAG AAATGAATGA AAAGGTGTCT
    TTCATAAAGG ACTCCTTATT GTCTTTGGAC AGCCAGGTGG GACACCTGCA GGATCTCTCT
    GTCCTGACAG TGGATACCTT AAAAGTCCTT TCTGCTGTTG ACACCTTACA AGAAGATGAG
    GCTCTCCTGG CCAACAGAAA GCATTCTACT TGCAGAAAAC TTCCCCACAG CTGGAGCAAT
    GTCATCTGTG CAGAGGTTCT GGGCAGCATG CCACTCTCTG AGAAGAAGAA GTATCAGTGT
    TATAGCATGC CCCCTTCTTT GCTGCGGAGC CTGGCCAGAG GCTGGTATCC CCCACGAGGG
    CAGAAAGGGC CCTTTCTTGA GATTACAGAT CAAGAGGCTT CAAATGTAAG AGATGACCAA
    GGAGAGCAAG AAACAAAAAA TCGTGTAGTT GCTTCTGGGG TGATGCTGAA CAGACAGGCA
    CACCCAAAGT ATGGCCAATA TCTCCTGGTC CCTTCTTATC TAGAACAAGT TTCTTATTCT
    GCAAAAACTG ACTTGCCTCT GTCCACACCA TCTGTGGAAG CAGGTACAGA TGGGCTGACA
    ACTGAACACG TCAGCCAGAC TGAGGTCCCT GTTCATCTGA TGTGGCAGAC CCCGGGTGTC
    TCAGCCAAGG GCCCCATGGT GGAAACCAAG GAGCAGTGTG AGTCACTTGG TCAGATACCA
    GCCAGACAAG ACAAGGGGGA ACGAGTGCTC CCCACTTTGA TTTGCACACC TGCACCCACG
    AGAGTGTCCT CCCTCCCTTC CCAGGTCATG AGCATGCACC CTGGAGGTGG GTATGTGAAC
    TGGGCATTTT CAGAAGGTGA TGAAACTGGT GTGTTTAGCA CCAAGAAGCA ATGTCAAATC
    AGCATGGCCT CCGCTTGTAA CCATGACTCC AGACTGAGTG AACAGGGCCA GAGGCAGAAC
    CGGGGCAGAT CCCTGTCTGA TAACTCGGCA AGACTGGCCC AGAGGAATTG CTCAGAAGTA
    CTTCCATGGC TCCAGCCAAA CACATCCTTT CGAATCAGTC TGCTTCGGAA AGACAAGCCC
    TTCCCTGGGA ATCAGAGCTT GAGATTACCC AAGGAGGAGA AGTTGAAGAT CTCTAAGATT
    AAAAATCTAT CAAAATTCTC AGAGATAGGA CAATCAAAAT GGATCAAAAC AAAATTGCTA
    ACCAAGGATA GGAAACTGTC AAAGAAGAAG AAGAAAACAC AAGCACTGCA GGTGCCAGTC
    ATAACAGTCA ACACCTGCTC TCAAAGTGAC CAGTTGAACC CAGAGCCTGG AGAAAAGATC
    TCAGAAGCAC ACAAGGGCAG CAAGAACTGG ATTCCAGTGT CCAAATTTAA TCAGATAAGT
    CTAGAATCTT ACATATATCA GAAAATGAAA ACTAAAGGAG CTGAACGACA TCCTGTACAA
    GTCAGTGATT ATGGAAGGCA GTCTCAAGAG GATCTTAGCA AAAGCTCTTT GTGGGATTCC
    AGGAACACCA CAGTCAATAG GAAGTCTCAG TTGAGAAGTT CAAATGGAGT TAACAAAATC
    TCAGACTCCT TAAAAAGCCC TCAAGAGCCT CACCATCATT ATTCAGCCAT TGAAAGGAAC
    AATTTAATGA GGCTTTCTCA GACCATACCG TTTACACCAA TCCAACTGTT TGCAGGAGAA
    GAAGTAACAG TCTACAGGCT GGAGGAGAGT TCTCCCTTGA ATCTGGATAA AAGCATGTCT
    TCTTGGTCCC AGCGGGGGAG ATCTGCTATG ATCCAGGTGT TGTCCCAAGA GGAGATGGAT
    GGTGGCCTTC GCAAAGCCAT GAGAGTCATT AGCACCTGGT CTGAGGATGA CATCCTCAAG
    CCAGGGCAGG TTTTCATTGT GAAGTCCTTT CTTCCTGAGG TTGTGCAGAC ATGGCACGAA
    ATCTTCCAGG AGAGTACTGT GCTTCATCTT TGCCTTCGGG AAATTCAACA ACAAAGAGCT
    GCTCAAAAAC TGATCTATAC CTTCAACCAA GTAAAACCAC AAACCATTCC CTACACTCCA
    AGGTTCCTGG AAGTTTTCTT AATTTACTGC CATTCAGCCC AGCAGTGGTT GACCATCGAG
    AAGTATATGA CGGGGGAGTT CCGGAAGTAC AACAACAACA ACGGTGATGA AATTGCCCCC
    AGCAACACCT TGGAGGAGCT GATGTTGGCT TTCTCTCACT GGACCTATGA GTATACTCGG
    GGAGAGCTGC TGGTTTTAGA TTTGCAAGGT GTTGGAGAAA ATTTGACGGA TCCATCTGTA
    ATAAAACCTG AAGACAAACA ATCAAGAGGA ATGGTGTTTG GACCAGCCAA TTTAGGGGAA
    GATGCAATTA GAAACTTCAT TGCAAAACAT CGTTGCAACT CCTGCTGCCG GAAGCTCAAA
    CTCCCGGACT TAAAAAGAAA TGACTACTCT CCTGGAAGGA TAAATTCTGC CTTTGGACTT
    GAAATCAAAA TAGAACCAGC TGAGGAGACT CCAGCGGGGG AAGAGGGTAG TAATTCTCCA
    GAAGACCTCA CACGATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806199.1
    CDS201..6281
    Translation

    Target ORF information:

    RefSeq Version XM_010807897.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAGAGGT CCCAGAAATC TTGGATTGAA GGAGTGTTCA ACAAGAGAGA ATGTAACAAA 
    ATCATACCCA GCTCTAAAGA CCCTCACAGA TGCACTGCAG GATGTCAGGT CTGTCAGAAT
    TTAATCAGGT GTTACTGTGG ACTATTGATT AGAGACCATC ATGGGATAGA TTATACTCGG
    ACTGTCTCAG CTGCCAGCGG TAGTGAAGAT GAACAGTGGT CTGTTGAAAA GCACACAACG
    AAAAGCCCTA CAGACACCTT TGGCACGATT AACTTCCAAC ATGGAGAGCA CATCCACCAC
    TCCAAGTATA TTAGAACTTC TTATGACACA AAGTTGGATC ACCTGTTACA TTTAATGTTG
    AAAGAGTGGA AGATGGAACT GCCAAAGCTG GTGATCTCCG TCCATGGTGG TATCCAGAAC
    TTCAAGATGC CCTCAAAACT TAAAGAGATC TTCAGCCAAG GTTTGGTCAA AGCTGCAGAG
    ACAACAGGAG CATGGATAAT AACTGAAGGC ATTAATACAG GTGTGTCTAA ACATGTTGGG
    GATGCCCTGA AAGCCCATTC CTCGCAGTCC TTGAGAAAAA TCTGGACAGT TGGAATCCCT
    CCTTGGGGTG TCATTGAGAA CCGGAAAGAT CTTATTGGAA GAGATGTAGT GTGCCTGTAC
    CAGACTCTGG GAAACCCCCT CAGCAAGCTC ACAACACTCA ACTGCATGCA CTCCCACTTT
    ATCCTGTCTG ATGATGGCAC TGTGGGCAAG TATGGCAATG AGATGAAGCT CAGGAGGAAC
    CTGGAAAAGT ACCTCTCTCT GCAGAAGATA CATAGTTGCT CTGGACAAGG CGTGCCCGTG
    GTGGGGCTGG TGGTGGAAGG TGGTCCCAAC GTCATCCTCT CCGTGTGGGA GACTGTGAAA
    GACAAAGACC CGGTGGTGGT GTGCGAAGGC ACAGGCAGGG CTGCTGACCT CCTGGCCTTC
    ACCCACAAAC ACTTAGCAGA TGAAGGGACC CTGCGTCCTC AGGTGAAGGA GGAGATCATC
    TGCATGATGC AGAACACCTT CAACTTTAGT CTTAAGCAGT CCAAGCACCT TTTCCAAATT
    CTGATGGAGT GTATGGTGCA CAGGGATTCT ATTACCATCT TTGATGCTGA TTCTGAGGAG
    TCACAGGACC TTGATTTAGC AATCCTAACA GCTCTGCTAA AGGGCACGAA TTTATCAGCA
    TCAGAACAAT TGAATCTGGC AATGGCTTGG GACAGAATGG ACATTGCCAA GAAACATATC
    CTAATTTATG GACAACACTG GAAGCCTGGC TCTCTAGAAC AAGCGATGTT AGATGCCTTG
    ATGATGGATC GAGTTGATTT TGTGAAGCTC TTAATAGAGT ATGGAGTGAA CCTCCATCGC
    TTTCTTACCA TCTCCCGACT GGAAGAGCTC TATAATACAA AACAAGGACC AACCAATATG
    CTCTTACATC ATCTTGTCCA AGATGTAAAA CAGCATACAC TTCTCTCAGG CTATAAGATA
    ACGCTGATTG ACATCGGATT GGTAATAGAA TACCTTATTG GTGGAGCATA TCGCAGCAGC
    TACACTAGAA AAACTTTCAG AGCCCTCTAC AACAACCTCT ACAGAAAACA TAAGCAAGTG
    ACCACTCTTG CTCAAAACCT GTCCCAGCCT GCTCTTCACC GAAGACGCTG CTCAGGAAAT
    AGAAAGGAGT CTACAGAGAG CACCCTGCAT TCCCAGTTCA TTAGAACTGC CCAACCTTAC
    AAAGTCAAGG AAAAGTCTGC AGCCCTTCAT AAATCAAGGA AGAAGTCAAA AGAAGAACAA
    AATATATCAG ATGACCCTGA TTCTACTGGC TTTATTTATC CTTATAATGA CCTGCTGGTG
    TGGGCTGTGC TAATGAAAAG GCAGAAGATG GCCATGTTCT TCTGGCAGCA TGGTGAGGAG
    GCCACGGTCA AGGCAGTGAT CGCTTGTATC CTCTACCGAG CAATGGCCCG GGAAGCCAAG
    GAGAGCAACA TGGTCGATGA TGCCTCTGAA GAACTGAAAA ATTACTCAAA ACAGTTTGGC
    CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA ATGAAAGAAT GGCCATGAAA
    CTGCTGACCT ACGAACTCAA GAACTGGAGC AACTCTACCT GTTTGAAGCT GGCTGTTTCT
    GGAGGGTTGA GGCCCTTTGT TTCACACACT TGTACCCAAA TGCTACTAAC TGATATGTGG
    ATGGGGCGTC TGAAAATGAG GAAAAACTCT TGGTTAAAGA TCATCATCAG TATTCTTTTC
    CCACCTGCCA TTTTGACACT GGAGTTTAAA AGCAAAGCAG AAATGTCACA TGTCCCACAG
    TCCCAGGACT TTACGTGGTA TAATGGTGAC CAGAACACCA GTACTCTCAA AGAAAATTCT
    TGTCAGAAAG ACTGTGACTT GGAAAGGGGA CCTGATGAGA AAACAGATGA AAGCCAGCAC
    TTTGATTTAA AGAGTGGGCT CCAAACCCTT CCTTGGACCA GGAAAGTCTA TGAGTTCTAC
    AATGCTCCAA TTGTCAAGTT TTGGTTTTAT ACGATGGTGT ACTTGGCATT CCTTATGCTG
    TTCACTTACA CCGTGTTGGT GGAGATGCAG CCCCAGCCCA GCGTGCAAGA GTGGCTTGTT
    ATTATCTACA TCTTCACCAA TGCCATTGAG AAAGTCAGGG AGGTCTGTGT TTCAGAACCT
    GGGAAGTTTA CTCAAAAGGT GAAGGTGTGG ATTAGCGAGT ACTGGAACTT AATGGAAACT
    GTGGCTATTG GCCTGTTTTT GGTGGGATTT GGCTTTCGGT GGGGTGCCCC ACCTTTCTAC
    ACGGCGGGAA GACTGATATA CTGCATCAAC ATCATATTCT GGTTCTCACG GCTCCTGGAC
    TTCTTTGCTG TGAATCAACA TGCAGGTCCC TATGTGACCA TGATTGCAAA AATGACAGCA
    AACATGTTCT ACATTGTGAT CATCATGGCC ATCGTCCTCC TGAGCTTTGG AGTGGCACGC
    AAAGCCATCC TTTCACCAAA GGAGCCCCCA TCCTGGACCC TTGCCCGAGA TATTGTATTT
    GAGCCATACT GGATGATATA TGGAGAAGTC TATGCTTCAG AGATAGATGT TTGTTCAAGC
    CAGCCGTCCT GCCCTCCTGG TTCTTTTCTT ACTCCATTCC TGCAAGCTGT CTACCTCTTC
    GTGCAATATA TCATCATGGT GAACCTGTTG ATTGCTTTCT TTAATAACGT TTACATAGAT
    CTGAAATCCA TTTCAAATAA CCTGTGGAAA TATAATCGCT ATCGTTACAT CATGACCTAC
    CATGAAAAGC CTTGGTTGCC CCCACCTTTT ATCCTGCTGA GTCACATTGG TCTCCTGCTT
    CGCCGTCTTT GCCAGCACCA AGCTCCAAAT GACCAAGAAG AGGGTGATGT TGGATTAAAA
    CTCTACCTAA GTAAGGAGGA TCTGAAAAAA CTTCACGATT TTGAAGAACA GTGTGTGGAA
    AAGTACTTCC ACGAGAAGAT GGAAGATCTG AATTGTAGTT GTGAGGAACA AATCCGAGTG
    ACATCAGAAA GGGTTACCGA GATGTACTTC CAAATGAAAG AAATGAATGA AAAGGTGTCT
    TTCATAAAGG ACTCCTTATT GTCTTTGGAC AGCCAGGTGG GACACCTGCA GGATCTCTCT
    GTCCTGACAG TGGATACCTT AAAAGTCCTT TCTGCTGTTG ACACCTTACA AGAAGATGAG
    GCTCTCCTGG CCAACAGAAA GCATTCTACT TGCAGAAAAC TTCCCCACAG CTGGAGCAAT
    GTCATCTGTG CAGAGGTTCT GGGCAGCATG CCACTCTCTG AGAAGAAGAA GTATCAGTGT
    TATAGCATGC CCCCTTCTTT GCTGCGGAGC CTGGCCAGAG GCTGGTATCC CCCACGAGGG
    CAGAAAGGGC CCTTTCTTGA GATTACAGAT CAAGAGGCTT CAAATGTAAG AGATGACCAA
    GGAGAGCAAG AAACAAAAAA TCGTGTAGTT GCTTCTGGGG TGATGCTGAA CAGACAGGCA
    CACCCAAAGT ATGGCCAATA TCTCCTGGTC CCTTCTTATC TAGAACAAGT TTCTTATTCT
    GCAAAAACTG ACTTGCCTCT GTCCACACCA TCTGTGGAAG CAGGTACAGA TGGGCTGACA
    ACTGAACACG TCAGCCAGAC TGAGGTCCCT GTTCATCTGA TGTGGCAGAC CCCGGGTGTC
    TCAGCCAAGG GCCCCATGGT GGAAACCAAG GAGCAGTGTG AGTCACTTGG TCAGATACCA
    GCCAGACAAG ACAAGGGGGA ACGAGTGCTC CCCACTTTGA TTTGCACACC TGCACCCACG
    AGAGTGTCCT CCCTCCCTTC CCAGGTCATG AGCATGCACC CTGGAGGTGG GTATGTGAAC
    TGGGCATTTT CAGAAGGTGA TGAAACTGGT GTGTTTAGCA CCAAGAAGCA ATGTCAAATC
    AGCATGGCCT CCGCTTGTAA CCATGACTCC AGACTGAGTG AACAGGGCCA GAGGCAGAAC
    CGGGGCAGAT CCCTGTCTGA TAACTCGGCA AGACTGGCCC AGAGGAATTG CTCAGAAGTA
    CTTCCATGGC TCCAGCCAAA CACATCCTTT CGAATCAGTC TGCTTCGGAA AGACAAGCCC
    TTCCCTGGGA ATCAGAGCTT GAGATTACCC AAGGAGGAGA AGTTGAAGAT CTCTAAGATT
    AAAAATCTAT CAAAATTCTC AGAGATAGGA CAATCAAAAT GGATCAAAAC AAAATTGCTA
    ACCAAGGATA GGAAACTGTC AAAGAAGAAG AAGAAAACAC AAGCACTGCA GGTGCCAGTC
    ATAACAGTCA ACACCTGCTC TCAAAGTGAC CAGTTGAACC CAGAGCCTGG AGAAAAGATC
    TCAGAAGCAC ACAAGGGCAG CAAGAACTGG ATTCCAGTGT CCAAATTTAA TCAGATAAGT
    CTAGAATCTT ACATATATCA GAAAATGAAA ACTAAAGGAG CTGAACGACA TCCTGTACAA
    GTCAGTGATT ATGGAAGGCA GTCTCAAGAG GATCTTAGCA AAAGCTCTTT GTGGGATTCC
    AGGAACACCA CAGTCAATAG GAAGTCTCAG TTGAGAAGTT CAAATGGAGT TAACAAAATC
    TCAGACTCCT TAAAAAGCCC TCAAGAGCCT CACCATCATT ATTCAGCCAT TGAAAGGAAC
    AATTTAATGA GGCTTTCTCA GACCATACCG TTTACACCAA TCCAACTGTT TGCAGGAGAA
    GAAGTAACAG TCTACAGGCT GGAGGAGAGT TCTCCCTTGA ATCTGGATAA AAGCATGTCT
    TCTTGGTCCC AGCGGGGGAG ATCTGCTATG ATCCAGGTGT TGTCCCAAGA GGAGATGGAT
    GGTGGCCTTC GCAAAGCCAT GAGAGTCATT AGCACCTGGT CTGAGGATGA CATCCTCAAG
    CCAGGGCAGG TTTTCATTGT GAAGTCCTTT CTTCCTGAGG TTGTGCAGAC ATGGCACGAA
    ATCTTCCAGG AGAGTACTGT GCTTCATCTT TGCCTTCGGG AAATTCAACA ACAAAGAGCT
    GCTCAAAAAC TGATCTATAC CTTCAACCAA GTAAAACCAC AAACCATTCC CTACACTCCA
    AGGTTCCTGG AAGTTTTCTT AATTTACTGC CATTCAGCCC AGCAGTGGTT GACCATCGAG
    AAGTATATGA CGGGGGAGTT CCGGAAGTAC AACAACAACA ACGGTGATGA AATTGCCCCC
    AGCAACACCT TGGAGGAGCT GATGTTGGCT TTCTCTCACT GGACCTATGA GTATACTCGG
    GGAGAGCTGC TGGTTTTAGA TTTGCAAGGT GTTGGAGAAA ATTTGACGGA TCCATCTGTA
    ATAAAACCTG AAGACAAACA ATCAAGAGGA ATGGTGTTTG GACCAGCCAA TTTAGGGGAA
    GATGCAATTA GAAACTTCAT TGCAAAACAT CGTTGCAACT CCTGCTGCCG GAAGCTCAAA
    CTCCCGGACT TAAAAAGAAA TGACTACTCT CCTGGAAGGA TAAATTCTGC CTTTGGACTT
    GAAATCAAAA TAGAACCAGC TGAGGAGACT CCAGCGGGGG AAGAGGGTAG TAATTCTCCA
    GAAGACCTCA CACGATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51415
    Clone ID Related Accession (Same CDS sequence) XM_010807897.1 , XM_010798670.1
    Accession Version XM_010798670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAGAGGT CCCAGAAATC TTGGATTGAA GGAGTGTTCA ACAAGAGAGA ATGTAACAAA 
    ATCATACCCA GCTCTAAAGA CCCTCACAGA TGCACTGCAG GATGTCAGGT CTGTCAGAAT
    TTAATCAGGT GTTACTGTGG ACTATTGATT AGAGACCATC ATGGGATAGA TTATACTCGG
    ACTGTCTCAG CTGCCAGCGG TAGTGAAGAT GAACAGTGGT CTGTTGAAAA GCACACAACG
    AAAAGCCCTA CAGACACCTT TGGCACGATT AACTTCCAAC ATGGAGAGCA CATCCACCAC
    TCCAAGTATA TTAGAACTTC TTATGACACA AAGTTGGATC ACCTGTTACA TTTAATGTTG
    AAAGAGTGGA AGATGGAACT GCCAAAGCTG GTGATCTCCG TCCATGGTGG TATCCAGAAC
    TTCAAGATGC CCTCAAAACT TAAAGAGATC TTCAGCCAAG GTTTGGTCAA AGCTGCAGAG
    ACAACAGGAG CATGGATAAT AACTGAAGGC ATTAATACAG GTGTGTCTAA ACATGTTGGG
    GATGCCCTGA AAGCCCATTC CTCGCAGTCC TTGAGAAAAA TCTGGACAGT TGGAATCCCT
    CCTTGGGGTG TCATTGAGAA CCGGAAAGAT CTTATTGGAA GAGATGTAGT GTGCCTGTAC
    CAGACTCTGG GAAACCCCCT CAGCAAGCTC ACAACACTCA ACTGCATGCA CTCCCACTTT
    ATCCTGTCTG ATGATGGCAC TGTGGGCAAG TATGGCAATG AGATGAAGCT CAGGAGGAAC
    CTGGAAAAGT ACCTCTCTCT GCAGAAGATA CATAGTTGCT CTGGACAAGG CGTGCCCGTG
    GTGGGGCTGG TGGTGGAAGG TGGTCCCAAC GTCATCCTCT CCGTGTGGGA GACTGTGAAA
    GACAAAGACC CGGTGGTGGT GTGCGAAGGC ACAGGCAGGG CTGCTGACCT CCTGGCCTTC
    ACCCACAAAC ACTTAGCAGA TGAAGGGACC CTGCGTCCTC AGGTGAAGGA GGAGATCATC
    TGCATGATGC AGAACACCTT CAACTTTAGT CTTAAGCAGT CCAAGCACCT TTTCCAAATT
    CTGATGGAGT GTATGGTGCA CAGGGATTCT ATTACCATCT TTGATGCTGA TTCTGAGGAG
    TCACAGGACC TTGATTTAGC AATCCTAACA GCTCTGCTAA AGGGCACGAA TTTATCAGCA
    TCAGAACAAT TGAATCTGGC AATGGCTTGG GACAGAATGG ACATTGCCAA GAAACATATC
    CTAATTTATG GACAACACTG GAAGCCTGGC TCTCTAGAAC AAGCGATGTT AGATGCCTTG
    ATGATGGATC GAGTTGATTT TGTGAAGCTC TTAATAGAGT ATGGAGTGAA CCTCCATCGC
    TTTCTTACCA TCTCCCGACT GGAAGAGCTC TATAATACAA AACAAGGACC AACCAATATG
    CTCTTACATC ATCTTGTCCA AGATGTAAAA CAGCATACAC TTCTCTCAGG CTATAAGATA
    ACGCTGATTG ACATCGGATT GGTAATAGAA TACCTTATTG GTGGAGCATA TCGCAGCAGC
    TACACTAGAA AAACTTTCAG AGCCCTCTAC AACAACCTCT ACAGAAAACA TAAGCAAGTG
    ACCACTCTTG CTCAAAACCT GTCCCAGCCT GCTCTTCACC GAAGACGCTG CTCAGGAAAT
    AGAAAGGAGT CTACAGAGAG CACCCTGCAT TCCCAGTTCA TTAGAACTGC CCAACCTTAC
    AAAGTCAAGG AAAAGTCTGC AGCCCTTCAT AAATCAAGGA AGAAGTCAAA AGAAGAACAA
    AATATATCAG ATGACCCTGA TTCTACTGGC TTTATTTATC CTTATAATGA CCTGCTGGTG
    TGGGCTGTGC TAATGAAAAG GCAGAAGATG GCCATGTTCT TCTGGCAGCA TGGTGAGGAG
    GCCACGGTCA AGGCAGTGAT CGCTTGTATC CTCTACCGAG CAATGGCCCG GGAAGCCAAG
    GAGAGCAACA TGGTCGATGA TGCCTCTGAA GAACTGAAAA ATTACTCAAA ACAGTTTGGC
    CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA ATGAAAGAAT GGCCATGAAA
    CTGCTGACCT ACGAACTCAA GAACTGGAGC AACTCTACCT GTTTGAAGCT GGCTGTTTCT
    GGAGGGTTGA GGCCCTTTGT TTCACACACT TGTACCCAAA TGCTACTAAC TGATATGTGG
    ATGGGGCGTC TGAAAATGAG GAAAAACTCT TGGTTAAAGA TCATCATCAG TATTCTTTTC
    CCACCTGCCA TTTTGACACT GGAGTTTAAA AGCAAAGCAG AAATGTCACA TGTCCCACAG
    TCCCAGGACT TTACGTGGTA TAATGGTGAC CAGAACACCA GTACTCTCAA AGAAAATTCT
    TGTCAGAAAG ACTGTGACTT GGAAAGGGGA CCTGATGAGA AAACAGATGA AAGCCAGCAC
    TTTGATTTAA AGAGTGGGCT CCAAACCCTT CCTTGGACCA GGAAAGTCTA TGAGTTCTAC
    AATGCTCCAA TTGTCAAGTT TTGGTTTTAT ACGATGGTGT ACTTGGCATT CCTTATGCTG
    TTCACTTACA CCGTGTTGGT GGAGATGCAG CCCCAGCCCA GCGTGCAAGA GTGGCTTGTT
    ATTATCTACA TCTTCACCAA TGCCATTGAG AAAGTCAGGG AGGTCTGTGT TTCAGAACCT
    GGGAAGTTTA CTCAAAAGGT GAAGGTGTGG ATTAGCGAGT ACTGGAACTT AATGGAAACT
    GTGGCTATTG GCCTGTTTTT GGTGGGATTT GGCTTTCGGT GGGGTGCCCC ACCTTTCTAC
    ACGGCGGGAA GACTGATATA CTGCATCAAC ATCATATTCT GGTTCTCACG GCTCCTGGAC
    TTCTTTGCTG TGAATCAACA TGCAGGTCCC TATGTGACCA TGATTGCAAA AATGACAGCA
    AACATGTTCT ACATTGTGAT CATCATGGCC ATCGTCCTCC TGAGCTTTGG AGTGGCACGC
    AAAGCCATCC TTTCACCAAA GGAGCCCCCA TCCTGGACCC TTGCCCGAGA TATTGTATTT
    GAGCCATACT GGATGATATA TGGAGAAGTC TATGCTTCAG AGATAGATGT TTGTTCAAGC
    CAGCCGTCCT GCCCTCCTGG TTCTTTTCTT ACTCCATTCC TGCAAGCTGT CTACCTCTTC
    GTGCAATATA TCATCATGGT GAACCTGTTG ATTGCTTTCT TTAATAACGT TTACATAGAT
    CTGAAATCCA TTTCAAATAA CCTGTGGAAA TATAATCGCT ATCGTTACAT CATGACCTAC
    CATGAAAAGC CTTGGTTGCC CCCACCTTTT ATCCTGCTGA GTCACATTGG TCTCCTGCTT
    CGCCGTCTTT GCCAGCACCA AGCTCCAAAT GACCAAGAAG AGGGTGATGT TGGATTAAAA
    CTCTACCTAA GTAAGGAGGA TCTGAAAAAA CTTCACGATT TTGAAGAACA GTGTGTGGAA
    AAGTACTTCC ACGAGAAGAT GGAAGATCTG AATTGTAGTT GTGAGGAACA AATCCGAGTG
    ACATCAGAAA GGGTTACCGA GATGTACTTC CAAATGAAAG AAATGAATGA AAAGGTGTCT
    TTCATAAAGG ACTCCTTATT GTCTTTGGAC AGCCAGGTGG GACACCTGCA GGATCTCTCT
    GTCCTGACAG TGGATACCTT AAAAGTCCTT TCTGCTGTTG ACACCTTACA AGAAGATGAG
    GCTCTCCTGG CCAACAGAAA GCATTCTACT TGCAGAAAAC TTCCCCACAG CTGGAGCAAT
    GTCATCTGTG CAGAGGTTCT GGGCAGCATG CCACTCTCTG AGAAGAAGAA GTATCAGTGT
    TATAGCATGC CCCCTTCTTT GCTGCGGAGC CTGGCCAGAG GCTGGTATCC CCCACGAGGG
    CAGAAAGGGC CCTTTCTTGA GATTACAGAT CAAGAGGCTT CAAATGTAAG AGATGACCAA
    GGAGAGCAAG AAACAAAAAA TCGTGTAGTT GCTTCTGGGG TGATGCTGAA CAGACAGGCA
    CACCCAAAGT ATGGCCAATA TCTCCTGGTC CCTTCTTATC TAGAACAAGT TTCTTATTCT
    GCAAAAACTG ACTTGCCTCT GTCCACACCA TCTGTGGAAG CAGGTACAGA TGGGCTGACA
    ACTGAACACG TCAGCCAGAC TGAGGTCCCT GTTCATCTGA TGTGGCAGAC CCCGGGTGTC
    TCAGCCAAGG GCCCCATGGT GGAAACCAAG GAGCAGTGTG AGTCACTTGG TCAGATACCA
    GCCAGACAAG ACAAGGGGGA ACGAGTGCTC CCCACTTTGA TTTGCACACC TGCACCCACG
    AGAGTGTCCT CCCTCCCTTC CCAGGTCATG AGCATGCACC CTGGAGGTGG GTATGTGAAC
    TGGGCATTTT CAGAAGGTGA TGAAACTGGT GTGTTTAGCA CCAAGAAGCA ATGTCAAATC
    AGCATGGCCT CCGCTTGTAA CCATGACTCC AGACTGAGTG AACAGGGCCA GAGGCAGAAC
    CGGGGCAGAT CCCTGTCTGA TAACTCGGCA AGACTGGCCC AGAGGAATTG CTCAGAAGTA
    CTTCCATGGC TCCAGCCAAA CACATCCTTT CGAATCAGTC TGCTTCGGAA AGACAAGCCC
    TTCCCTGGGA ATCAGAGCTT GAGATTACCC AAGGAGGAGA AGTTGAAGAT CTCTAAGATT
    AAAAATCTAT CAAAATTCTC AGAGATAGGA CAATCAAAAT GGATCAAAAC AAAATTGCTA
    ACCAAGGATA GGAAACTGTC AAAGAAGAAG AAGAAAACAC AAGCACTGCA GGTGCCAGTC
    ATAACAGTCA ACACCTGCTC TCAAAGTGAC CAGTTGAACC CAGAGCCTGG AGAAAAGATC
    TCAGAAGCAC ACAAGGGCAG CAAGAACTGG ATTCCAGTGT CCAAATTTAA TCAGATAAGT
    CTAGAATCTT ACATATATCA GAAAATGAAA ACTAAAGGAG CTGAACGACA TCCTGTACAA
    GTCAGTGATT ATGGAAGGCA GTCTCAAGAG GATCTTAGCA AAAGCTCTTT GTGGGATTCC
    AGGAACACCA CAGTCAATAG GAAGTCTCAG TTGAGAAGTT CAAATGGAGT TAACAAAATC
    TCAGACTCCT TAAAAAGCCC TCAAGAGCCT CACCATCATT ATTCAGCCAT TGAAAGGAAC
    AATTTAATGA GGCTTTCTCA GACCATACCG TTTACACCAA TCCAACTGTT TGCAGGAGAA
    GAAGTAACAG TCTACAGGCT GGAGGAGAGT TCTCCCTTGA ATCTGGATAA AAGCATGTCT
    TCTTGGTCCC AGCGGGGGAG ATCTGCTATG ATCCAGGTGT TGTCCCAAGA GGAGATGGAT
    GGTGGCCTTC GCAAAGCCAT GAGAGTCATT AGCACCTGGT CTGAGGATGA CATCCTCAAG
    CCAGGGCAGG TTTTCATTGT GAAGTCCTTT CTTCCTGAGG TTGTGCAGAC ATGGCACGAA
    ATCTTCCAGG AGAGTACTGT GCTTCATCTT TGCCTTCGGG AAATTCAACA ACAAAGAGCT
    GCTCAAAAAC TGATCTATAC CTTCAACCAA GTAAAACCAC AAACCATTCC CTACACTCCA
    AGGTTCCTGG AAGTTTTCTT AATTTACTGC CATTCAGCCC AGCAGTGGTT GACCATCGAG
    AAGTATATGA CGGGGGAGTT CCGGAAGTAC AACAACAACA ACGGTGATGA AATTGCCCCC
    AGCAACACCT TGGAGGAGCT GATGTTGGCT TTCTCTCACT GGACCTATGA GTATACTCGG
    GGAGAGCTGC TGGTTTTAGA TTTGCAAGGT GTTGGAGAAA ATTTGACGGA TCCATCTGTA
    ATAAAACCTG AAGACAAACA ATCAAGAGGA ATGGTGTTTG GACCAGCCAA TTTAGGGGAA
    GATGCAATTA GAAACTTCAT TGCAAAACAT CGTTGCAACT CCTGCTGCCG GAAGCTCAAA
    CTCCCGGACT TAAAAAGAAA TGACTACTCT CCTGGAAGGA TAAATTCTGC CTTTGGACTT
    GAAATCAAAA TAGAACCAGC TGAGGAGACT CCAGCGGGGG AAGAGGGTAG TAATTCTCCA
    GAAGACCTCA CACGATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796972.1
    CDS201..6281
    Translation

    Target ORF information:

    RefSeq Version XM_010798670.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAGAGGT CCCAGAAATC TTGGATTGAA GGAGTGTTCA ACAAGAGAGA ATGTAACAAA 
    ATCATACCCA GCTCTAAAGA CCCTCACAGA TGCACTGCAG GATGTCAGGT CTGTCAGAAT
    TTAATCAGGT GTTACTGTGG ACTATTGATT AGAGACCATC ATGGGATAGA TTATACTCGG
    ACTGTCTCAG CTGCCAGCGG TAGTGAAGAT GAACAGTGGT CTGTTGAAAA GCACACAACG
    AAAAGCCCTA CAGACACCTT TGGCACGATT AACTTCCAAC ATGGAGAGCA CATCCACCAC
    TCCAAGTATA TTAGAACTTC TTATGACACA AAGTTGGATC ACCTGTTACA TTTAATGTTG
    AAAGAGTGGA AGATGGAACT GCCAAAGCTG GTGATCTCCG TCCATGGTGG TATCCAGAAC
    TTCAAGATGC CCTCAAAACT TAAAGAGATC TTCAGCCAAG GTTTGGTCAA AGCTGCAGAG
    ACAACAGGAG CATGGATAAT AACTGAAGGC ATTAATACAG GTGTGTCTAA ACATGTTGGG
    GATGCCCTGA AAGCCCATTC CTCGCAGTCC TTGAGAAAAA TCTGGACAGT TGGAATCCCT
    CCTTGGGGTG TCATTGAGAA CCGGAAAGAT CTTATTGGAA GAGATGTAGT GTGCCTGTAC
    CAGACTCTGG GAAACCCCCT CAGCAAGCTC ACAACACTCA ACTGCATGCA CTCCCACTTT
    ATCCTGTCTG ATGATGGCAC TGTGGGCAAG TATGGCAATG AGATGAAGCT CAGGAGGAAC
    CTGGAAAAGT ACCTCTCTCT GCAGAAGATA CATAGTTGCT CTGGACAAGG CGTGCCCGTG
    GTGGGGCTGG TGGTGGAAGG TGGTCCCAAC GTCATCCTCT CCGTGTGGGA GACTGTGAAA
    GACAAAGACC CGGTGGTGGT GTGCGAAGGC ACAGGCAGGG CTGCTGACCT CCTGGCCTTC
    ACCCACAAAC ACTTAGCAGA TGAAGGGACC CTGCGTCCTC AGGTGAAGGA GGAGATCATC
    TGCATGATGC AGAACACCTT CAACTTTAGT CTTAAGCAGT CCAAGCACCT TTTCCAAATT
    CTGATGGAGT GTATGGTGCA CAGGGATTCT ATTACCATCT TTGATGCTGA TTCTGAGGAG
    TCACAGGACC TTGATTTAGC AATCCTAACA GCTCTGCTAA AGGGCACGAA TTTATCAGCA
    TCAGAACAAT TGAATCTGGC AATGGCTTGG GACAGAATGG ACATTGCCAA GAAACATATC
    CTAATTTATG GACAACACTG GAAGCCTGGC TCTCTAGAAC AAGCGATGTT AGATGCCTTG
    ATGATGGATC GAGTTGATTT TGTGAAGCTC TTAATAGAGT ATGGAGTGAA CCTCCATCGC
    TTTCTTACCA TCTCCCGACT GGAAGAGCTC TATAATACAA AACAAGGACC AACCAATATG
    CTCTTACATC ATCTTGTCCA AGATGTAAAA CAGCATACAC TTCTCTCAGG CTATAAGATA
    ACGCTGATTG ACATCGGATT GGTAATAGAA TACCTTATTG GTGGAGCATA TCGCAGCAGC
    TACACTAGAA AAACTTTCAG AGCCCTCTAC AACAACCTCT ACAGAAAACA TAAGCAAGTG
    ACCACTCTTG CTCAAAACCT GTCCCAGCCT GCTCTTCACC GAAGACGCTG CTCAGGAAAT
    AGAAAGGAGT CTACAGAGAG CACCCTGCAT TCCCAGTTCA TTAGAACTGC CCAACCTTAC
    AAAGTCAAGG AAAAGTCTGC AGCCCTTCAT AAATCAAGGA AGAAGTCAAA AGAAGAACAA
    AATATATCAG ATGACCCTGA TTCTACTGGC TTTATTTATC CTTATAATGA CCTGCTGGTG
    TGGGCTGTGC TAATGAAAAG GCAGAAGATG GCCATGTTCT TCTGGCAGCA TGGTGAGGAG
    GCCACGGTCA AGGCAGTGAT CGCTTGTATC CTCTACCGAG CAATGGCCCG GGAAGCCAAG
    GAGAGCAACA TGGTCGATGA TGCCTCTGAA GAACTGAAAA ATTACTCAAA ACAGTTTGGC
    CAGCTTGCCC TGGATGTGTT GGAGAAGGCA TTCAAACAGA ATGAAAGAAT GGCCATGAAA
    CTGCTGACCT ACGAACTCAA GAACTGGAGC AACTCTACCT GTTTGAAGCT GGCTGTTTCT
    GGAGGGTTGA GGCCCTTTGT TTCACACACT TGTACCCAAA TGCTACTAAC TGATATGTGG
    ATGGGGCGTC TGAAAATGAG GAAAAACTCT TGGTTAAAGA TCATCATCAG TATTCTTTTC
    CCACCTGCCA TTTTGACACT GGAGTTTAAA AGCAAAGCAG AAATGTCACA TGTCCCACAG
    TCCCAGGACT TTACGTGGTA TAATGGTGAC CAGAACACCA GTACTCTCAA AGAAAATTCT
    TGTCAGAAAG ACTGTGACTT GGAAAGGGGA CCTGATGAGA AAACAGATGA AAGCCAGCAC
    TTTGATTTAA AGAGTGGGCT CCAAACCCTT CCTTGGACCA GGAAAGTCTA TGAGTTCTAC
    AATGCTCCAA TTGTCAAGTT TTGGTTTTAT ACGATGGTGT ACTTGGCATT CCTTATGCTG
    TTCACTTACA CCGTGTTGGT GGAGATGCAG CCCCAGCCCA GCGTGCAAGA GTGGCTTGTT
    ATTATCTACA TCTTCACCAA TGCCATTGAG AAAGTCAGGG AGGTCTGTGT TTCAGAACCT
    GGGAAGTTTA CTCAAAAGGT GAAGGTGTGG ATTAGCGAGT ACTGGAACTT AATGGAAACT
    GTGGCTATTG GCCTGTTTTT GGTGGGATTT GGCTTTCGGT GGGGTGCCCC ACCTTTCTAC
    ACGGCGGGAA GACTGATATA CTGCATCAAC ATCATATTCT GGTTCTCACG GCTCCTGGAC
    TTCTTTGCTG TGAATCAACA TGCAGGTCCC TATGTGACCA TGATTGCAAA AATGACAGCA
    AACATGTTCT ACATTGTGAT CATCATGGCC ATCGTCCTCC TGAGCTTTGG AGTGGCACGC
    AAAGCCATCC TTTCACCAAA GGAGCCCCCA TCCTGGACCC TTGCCCGAGA TATTGTATTT
    GAGCCATACT GGATGATATA TGGAGAAGTC TATGCTTCAG AGATAGATGT TTGTTCAAGC
    CAGCCGTCCT GCCCTCCTGG TTCTTTTCTT ACTCCATTCC TGCAAGCTGT CTACCTCTTC
    GTGCAATATA TCATCATGGT GAACCTGTTG ATTGCTTTCT TTAATAACGT TTACATAGAT
    CTGAAATCCA TTTCAAATAA CCTGTGGAAA TATAATCGCT ATCGTTACAT CATGACCTAC
    CATGAAAAGC CTTGGTTGCC CCCACCTTTT ATCCTGCTGA GTCACATTGG TCTCCTGCTT
    CGCCGTCTTT GCCAGCACCA AGCTCCAAAT GACCAAGAAG AGGGTGATGT TGGATTAAAA
    CTCTACCTAA GTAAGGAGGA TCTGAAAAAA CTTCACGATT TTGAAGAACA GTGTGTGGAA
    AAGTACTTCC ACGAGAAGAT GGAAGATCTG AATTGTAGTT GTGAGGAACA AATCCGAGTG
    ACATCAGAAA GGGTTACCGA GATGTACTTC CAAATGAAAG AAATGAATGA AAAGGTGTCT
    TTCATAAAGG ACTCCTTATT GTCTTTGGAC AGCCAGGTGG GACACCTGCA GGATCTCTCT
    GTCCTGACAG TGGATACCTT AAAAGTCCTT TCTGCTGTTG ACACCTTACA AGAAGATGAG
    GCTCTCCTGG CCAACAGAAA GCATTCTACT TGCAGAAAAC TTCCCCACAG CTGGAGCAAT
    GTCATCTGTG CAGAGGTTCT GGGCAGCATG CCACTCTCTG AGAAGAAGAA GTATCAGTGT
    TATAGCATGC CCCCTTCTTT GCTGCGGAGC CTGGCCAGAG GCTGGTATCC CCCACGAGGG
    CAGAAAGGGC CCTTTCTTGA GATTACAGAT CAAGAGGCTT CAAATGTAAG AGATGACCAA
    GGAGAGCAAG AAACAAAAAA TCGTGTAGTT GCTTCTGGGG TGATGCTGAA CAGACAGGCA
    CACCCAAAGT ATGGCCAATA TCTCCTGGTC CCTTCTTATC TAGAACAAGT TTCTTATTCT
    GCAAAAACTG ACTTGCCTCT GTCCACACCA TCTGTGGAAG CAGGTACAGA TGGGCTGACA
    ACTGAACACG TCAGCCAGAC TGAGGTCCCT GTTCATCTGA TGTGGCAGAC CCCGGGTGTC
    TCAGCCAAGG GCCCCATGGT GGAAACCAAG GAGCAGTGTG AGTCACTTGG TCAGATACCA
    GCCAGACAAG ACAAGGGGGA ACGAGTGCTC CCCACTTTGA TTTGCACACC TGCACCCACG
    AGAGTGTCCT CCCTCCCTTC CCAGGTCATG AGCATGCACC CTGGAGGTGG GTATGTGAAC
    TGGGCATTTT CAGAAGGTGA TGAAACTGGT GTGTTTAGCA CCAAGAAGCA ATGTCAAATC
    AGCATGGCCT CCGCTTGTAA CCATGACTCC AGACTGAGTG AACAGGGCCA GAGGCAGAAC
    CGGGGCAGAT CCCTGTCTGA TAACTCGGCA AGACTGGCCC AGAGGAATTG CTCAGAAGTA
    CTTCCATGGC TCCAGCCAAA CACATCCTTT CGAATCAGTC TGCTTCGGAA AGACAAGCCC
    TTCCCTGGGA ATCAGAGCTT GAGATTACCC AAGGAGGAGA AGTTGAAGAT CTCTAAGATT
    AAAAATCTAT CAAAATTCTC AGAGATAGGA CAATCAAAAT GGATCAAAAC AAAATTGCTA
    ACCAAGGATA GGAAACTGTC AAAGAAGAAG AAGAAAACAC AAGCACTGCA GGTGCCAGTC
    ATAACAGTCA ACACCTGCTC TCAAAGTGAC CAGTTGAACC CAGAGCCTGG AGAAAAGATC
    TCAGAAGCAC ACAAGGGCAG CAAGAACTGG ATTCCAGTGT CCAAATTTAA TCAGATAAGT
    CTAGAATCTT ACATATATCA GAAAATGAAA ACTAAAGGAG CTGAACGACA TCCTGTACAA
    GTCAGTGATT ATGGAAGGCA GTCTCAAGAG GATCTTAGCA AAAGCTCTTT GTGGGATTCC
    AGGAACACCA CAGTCAATAG GAAGTCTCAG TTGAGAAGTT CAAATGGAGT TAACAAAATC
    TCAGACTCCT TAAAAAGCCC TCAAGAGCCT CACCATCATT ATTCAGCCAT TGAAAGGAAC
    AATTTAATGA GGCTTTCTCA GACCATACCG TTTACACCAA TCCAACTGTT TGCAGGAGAA
    GAAGTAACAG TCTACAGGCT GGAGGAGAGT TCTCCCTTGA ATCTGGATAA AAGCATGTCT
    TCTTGGTCCC AGCGGGGGAG ATCTGCTATG ATCCAGGTGT TGTCCCAAGA GGAGATGGAT
    GGTGGCCTTC GCAAAGCCAT GAGAGTCATT AGCACCTGGT CTGAGGATGA CATCCTCAAG
    CCAGGGCAGG TTTTCATTGT GAAGTCCTTT CTTCCTGAGG TTGTGCAGAC ATGGCACGAA
    ATCTTCCAGG AGAGTACTGT GCTTCATCTT TGCCTTCGGG AAATTCAACA ACAAAGAGCT
    GCTCAAAAAC TGATCTATAC CTTCAACCAA GTAAAACCAC AAACCATTCC CTACACTCCA
    AGGTTCCTGG AAGTTTTCTT AATTTACTGC CATTCAGCCC AGCAGTGGTT GACCATCGAG
    AAGTATATGA CGGGGGAGTT CCGGAAGTAC AACAACAACA ACGGTGATGA AATTGCCCCC
    AGCAACACCT TGGAGGAGCT GATGTTGGCT TTCTCTCACT GGACCTATGA GTATACTCGG
    GGAGAGCTGC TGGTTTTAGA TTTGCAAGGT GTTGGAGAAA ATTTGACGGA TCCATCTGTA
    ATAAAACCTG AAGACAAACA ATCAAGAGGA ATGGTGTTTG GACCAGCCAA TTTAGGGGAA
    GATGCAATTA GAAACTTCAT TGCAAAACAT CGTTGCAACT CCTGCTGCCG GAAGCTCAAA
    CTCCCGGACT TAAAAAGAAA TGACTACTCT CCTGGAAGGA TAAATTCTGC CTTTGGACTT
    GAAATCAAAA TAGAACCAGC TGAGGAGACT CCAGCGGGGG AAGAGGGTAG TAATTCTCCA
    GAAGACCTCA CACGATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51416
    Clone ID Related Accession (Same CDS sequence) XM_015464530.1 , XM_015472506.1
    Accession Version XM_015464530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GGTAATTTCC CTGTTGAACT AAAGAAGGAG
    GCTTCAGGAA GCAGAGGATT TGATCACTGG ACAACGCTTT TAGAGGTTCA GTCAAACCTG
    AGACTTAAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320016.1
    CDS750..6827
    Translation

    Target ORF information:

    RefSeq Version XM_015464530.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GGTAATTTCC CTGTTGAACT AAAGAAGGAG
    GCTTCAGGAA GCAGAGGATT TGATCACTGG ACAACGCTTT TAGAGGTTCA GTCAAACCTG
    AGACTTAAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51416
    Clone ID Related Accession (Same CDS sequence) XM_015464530.1 , XM_015472506.1
    Accession Version XM_015472506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GGTAATTTCC CTGTTGAACT AAAGAAGGAG
    GCTTCAGGAA GCAGAGGATT TGATCACTGG ACAACGCTTT TAGAGGTTCA GTCAAACCTG
    AGACTTAAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327992.1
    CDS750..6827
    Translation

    Target ORF information:

    RefSeq Version XM_015472506.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAGCGCTC CTTCCGCGGC GGAGCGCTTA CAGTCCCAGA AATCTTGGAT TGAAGGAGTG 
    TTCAACAAGA GAGAATGTAA CAAAATCATA CCCAGCTCTA AAGACCCTCA CAGATGCACT
    GCAGGATGTC AGGTCTGTCA GAATTTAATC AGGTGTTACT GTGGACTATT GATTAGAGAC
    CATCATGGGA TAGATTATAC TCGGACTGTC TCAGCTGCCA GCGGTAGTGA AGATGAACAG
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCTACAGACA CCTTTGGCAC GATTAACTTC
    CAACATGGAG AGCACATCCA CCACTCCAAG TATATTAGAA CTTCTTATGA CACAAAGTTG
    GATCACCTGT TACATTTAAT GTTGAAAGAG TGGAAGATGG AACTGCCAAA GCTGGTGATC
    TCCGTCCATG GTGGTATCCA GAACTTCAAG ATGCCCTCAA AACTTAAAGA GATCTTCAGC
    CAAGGTTTGG TCAAAGCTGC AGAGACAACA GGAGCATGGA TAATAACTGA AGGCATTAAT
    ACAGGTGTGT CTAAACATGT TGGGGATGCC CTGAAAGCCC ATTCCTCGCA GTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCGGAA AGATCTTATT
    GGAAGAGATG TAGTGTGCCT GTACCAGACT CTGGGAAACC CCCTCAGCAA GCTCACAACA
    CTCAACTGCA TGCACTCCCA CTTTATCCTG TCTGATGATG GCACTGTGGG CAAGTATGGC
    AATGAGATGA AGCTCAGGAG GAACCTGGAA AAGTACCTCT CTCTGCAGAA GATACATAGT
    TGCTCTGGAC AAGGCGTGCC CGTGGTGGGG CTGGTGGTGG AAGGTGGTCC CAACGTCATC
    CTCTCCGTGT GGGAGACTGT GAAAGACAAA GACCCGGTGG TGGTGTGCGA AGGCACAGGC
    AGGGCTGCTG ACCTCCTGGC CTTCACCCAC AAACACTTAG CAGATGAAGG GACCCTGCGT
    CCTCAGGTGA AGGAGGAGAT CATCTGCATG ATGCAGAACA CCTTCAACTT TAGTCTTAAG
    CAGTCCAAGC ACCTTTTCCA AATTCTGATG GAGTGTATGG TGCACAGGGA TTCTATTACC
    ATCTTTGATG CTGATTCTGA GGAGTCACAG GACCTTGATT TAGCAATCCT AACAGCTCTG
    CTAAAGGGCA CGAATTTATC AGCATCAGAA CAATTGAATC TGGCAATGGC TTGGGACAGA
    ATGGACATTG CCAAGAAACA TATCCTAATT TATGGACAAC ACTGGAAGCC TGGCTCTCTA
    GAACAAGCGA TGTTAGATGC CTTGATGATG GATCGAGTTG ATTTTGTGAA GCTCTTAATA
    GAGTATGGAG TGAACCTCCA TCGCTTTCTT ACCATCTCCC GACTGGAAGA GCTCTATAAT
    ACAAAACAAG GACCAACCAA TATGCTCTTA CATCATCTTG TCCAAGATGT AAAACAGCAT
    ACACTTCTCT CAGGCTATAA GATAACGCTG ATTGACATCG GATTGGTAAT AGAATACCTT
    ATTGGTGGAG CATATCGCAG CAGCTACACT AGAAAAACTT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AACATAAGCA AGTGACCACT CTTGCTCAAA ACCTGTCCCA GCCTGCTCTT
    CACCGAAGAC GCTGCTCAGG AAATAGAAAG GAGTCTACAG AGAGCACCCT GCATTCCCAG
    TTCATTAGAA CTGCCCAACC TTACAAAGTC AAGGAAAAGT CTGCAGCCCT TCATAAATCA
    AGGAAGAAGT CAAAAGAAGA ACAAAATATA TCAGATGACC CTGATTCTAC TGGCTTTATT
    TATCCTTATA ATGACCTGCT GGTGTGGGCT GTGCTAATGA AAAGGCAGAA GATGGCCATG
    TTCTTCTGGC AGCATGGTGA GGAGGCCACG GTCAAGGCAG TGATCGCTTG TATCCTCTAC
    CGAGCAATGG CCCGGGAAGC CAAGGAGAGC AACATGGTCG ATGATGCCTC TGAAGAACTG
    AAAAATTACT CAAAACAGTT TGGCCAGCTT GCCCTGGATG TGTTGGAGAA GGCATTCAAA
    CAGAATGAAA GAATGGCCAT GAAACTGCTG ACCTACGAAC TCAAGAACTG GAGCAACTCT
    ACCTGTTTGA AGCTGGCTGT TTCTGGAGGG TTGAGGCCCT TTGTTTCACA CACTTGTACC
    CAAATGCTAC TAACTGATAT GTGGATGGGG CGTCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATCATCA TCAGTATTCT TTTCCCACCT GCCATTTTGA CACTGGAGTT TAAAAGCAAA
    GCAGAAATGT CACATGTCCC ACAGTCCCAG GACTTTACGT GGTATAATGG TGACCAGAAC
    ACCAGTACTC TCAAAGAAAA TTCTTGTCAG AAAGACTGTG ACTTGGAAAG GGGACCTGAT
    GAGAAAACAG ATGAAAGCCA GCACTTTGAT TTAAAGAGTG GGCTCCAAAC CCTTCCTTGG
    ACCAGGAAAG TCTATGAGTT CTACAATGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GTGTACTTGG CATTCCTTAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AAGAGTGGCT TGTTATTATC TACATCTTCA CCAATGCCAT TGAGAAAGTC
    AGGGAGGTCT GTGTTTCAGA ACCTGGGAAG TTTACTCAAA AGGTGAAGGT GTGGATTAGC
    GAGTACTGGA ACTTAATGGA AACTGTGGCT ATTGGCCTGT TTTTGGTGGG ATTTGGCTTT
    CGGTGGGGTG CCCCACCTTT CTACACGGCG GGAAGACTGA TATACTGCAT CAACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCCTATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTACATTG TGATCATCAT GGCCATCGTC
    CTCCTGAGCT TTGGAGTGGC ACGCAAAGCC ATCCTTTCAC CAAAGGAGCC CCCATCCTGG
    ACCCTTGCCC GAGATATTGT ATTTGAGCCA TACTGGATGA TATATGGAGA AGTCTATGCT
    TCAGAGATAG ATGTTTGTTC AAGCCAGCCG TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCCTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTTAATA ACGTTTACAT AGATCTGAAA TCCATTTCAA ATAACCTGTG GAAATATAAT
    CGCTATCGTT ACATCATGAC CTACCATGAA AAGCCTTGGT TGCCCCCACC TTTTATCCTG
    CTGAGTCACA TTGGTCTCCT GCTTCGCCGT CTTTGCCAGC ACCAAGCTCC AAATGACCAA
    GAAGAGGGTG ATGTTGGATT AAAACTCTAC CTAAGTAAGG AGGATCTGAA AAAACTTCAC
    GATTTTGAAG AACAGTGTGT GGAAAAGTAC TTCCACGAGA AGATGGAAGA TCTGAATTGT
    AGTTGTGAGG AACAAATCCG AGTGACATCA GAAAGGGTTA CCGAGATGTA CTTCCAAATG
    AAAGAAATGA ATGAAAAGGT GTCTTTCATA AAGGACTCCT TATTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGTCCTG ACAGTGGATA CCTTAAAAGT CCTTTCTGCT
    GTTGACACCT TACAAGAAGA TGAGGCTCTC CTGGCCAACA GAAAGCATTC TACTTGCAGA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTGGGCAG CATGCCACTC
    TCTGAGAAGA AGAAGTATCA GTGTTATAGC ATGCCCCCTT CTTTGCTGCG GAGCCTGGCC
    AGAGGCTGGT ATCCCCCACG AGGGCAGAAA GGGCCCTTTC TTGAGATTAC AGATCAAGAG
    GCTTCAAATG TAAGAGATGA CCAAGGAGAG CAAGAAACAA AAAATCGTGT AGTTGCTTCT
    GGGGTGATGC TGAACAGACA GGCACACCCA AAGTATGGCC AATATCTCCT GGTCCCTTCT
    TATCTAGAAC AAGTTTCTTA TTCTGCAAAA ACTGACTTGC CTCTGTCCAC ACCATCTGTG
    GAAGCAGGTA CAGATGGGCT GACAACTGAA CACGTCAGCC AGACTGAGGT CCCTGTTCAT
    CTGATGTGGC AGACCCCGGG TGTCTCAGCC AAGGGCCCCA TGGTGGAAAC CAAGGAGCAG
    TGTGAGTCAC TTGGTCAGAT ACCAGCCAGA CAAGACAAGG GGGAACGAGT GCTCCCCACT
    TTGATTTGCA CACCTGCACC CACGAGAGTG TCCTCCCTCC CTTCCCAGGT CATGAGCATG
    CACCCTGGAG GTGGGTATGT GAACTGGGCA TTTTCAGAAG GTGATGAAAC TGGTGTGTTT
    AGCACCAAGA AGCAATGTCA AATCAGCATG GCCTCCGCTT GTAACCATGA CTCCAGACTG
    AGTGAACAGG GCCAGAGGCA GAACCGGGGC AGATCCCTGT CTGATAACTC GGCAAGACTG
    GCCCAGAGGA ATTGCTCAGA AGTACTTCCA TGGCTCCAGC CAAACACATC CTTTCGAATC
    AGTCTGCTTC GGAAAGACAA GCCCTTCCCT GGGAATCAGA GCTTGAGATT ACCCAAGGAG
    GAGAAGTTGA AGATCTCTAA GATTAAAAAT CTATCAAAAT TCTCAGAGAT AGGACAATCA
    AAATGGATCA AAACAAAATT GCTAACCAAG GATAGGAAAC TGTCAAAGAA GAAGAAGAAA
    ACACAAGCAC TGCAGGTGCC AGTCATAACA GTCAACACCT GCTCTCAAAG TGACCAGTTG
    AACCCAGAGC CTGGAGAAAA GATCTCAGAA GCACACAAGG GCAGCAAGAA CTGGATTCCA
    GTGTCCAAAT TTAATCAGAT AAGTCTAGAA TCTTACATAT ATCAGAAAAT GAAAACTAAA
    GGAGCTGAAC GACATCCTGT ACAAGTCAGT GATTATGGAA GGCAGTCTCA AGAGGATCTT
    AGCAAAAGCT CTTTGTGGGA TTCCAGGAAC ACCACAGTCA ATAGGAAGTC TCAGTTGAGA
    AGTTCAAATG GAGTTAACAA AATCTCAGAC TCCTTAAAAA GCCCTCAAGA GCCTCACCAT
    CATTATTCAG CCATTGAAAG GAACAATTTA ATGAGGCTTT CTCAGACCAT ACCGTTTACA
    CCAATCCAAC TGTTTGCAGG AGAAGAAGTA ACAGTCTACA GGCTGGAGGA GAGTTCTCCC
    TTGAATCTGG ATAAAAGCAT GTCTTCTTGG TCCCAGCGGG GGAGATCTGC TATGATCCAG
    GTGTTGTCCC AAGAGGAGAT GGATGGTGGC CTTCGCAAAG CCATGAGAGT CATTAGCACC
    TGGTCTGAGG ATGACATCCT CAAGCCAGGG CAGGTTTTCA TTGTGAAGTC CTTTCTTCCT
    GAGGTTGTGC AGACATGGCA CGAAATCTTC CAGGAGAGTA CTGTGCTTCA TCTTTGCCTT
    CGGGAAATTC AACAACAAAG AGCTGCTCAA AAACTGATCT ATACCTTCAA CCAAGTAAAA
    CCACAAACCA TTCCCTACAC TCCAAGGTTC CTGGAAGTTT TCTTAATTTA CTGCCATTCA
    GCCCAGCAGT GGTTGACCAT CGAGAAGTAT ATGACGGGGG AGTTCCGGAA GTACAACAAC
    AACAACGGTG ATGAAATTGC CCCCAGCAAC ACCTTGGAGG AGCTGATGTT GGCTTTCTCT
    CACTGGACCT ATGAGTATAC TCGGGGAGAG CTGCTGGTTT TAGATTTGCA AGGTGTTGGA
    GAAAATTTGA CGGATCCATC TGTAATAAAA CCTGAAGACA AACAATCAAG AGGAATGGTG
    TTTGGACCAG CCAATTTAGG GGAAGATGCA ATTAGAAACT TCATTGCAAA ACATCGTTGC
    AACTCCTGCT GCCGGAAGCT CAAACTCCCG GGTAATTTCC CTGTTGAACT AAAGAAGGAG
    GCTTCAGGAA GCAGAGGATT TGATCACTGG ACAACGCTTT TAGAGGTTCA GTCAAACCTG
    AGACTTAAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.