RICTOR cDNA ORF clone, Bos taurus(cattle)

The following RICTOR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RICTOR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228023 XM_024981299.1
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa228022 XM_024981297.1
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa36073 NM_001144096.3
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa30669 XM_015459092.1
Latest version!
Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa30669 XM_024981298.1
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa51387 XM_010816809.3
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa51388 XM_010816811.3
Latest version!
Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa51388 XM_010816811.2 Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa51387 XM_010816809.2 Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228023
    Clone ID Related Accession (Same CDS sequence) XM_024981299.1
    Accession Version XM_024981299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGAATCA TAGCAACATT CCGGTCATGG GCAGGTATTA TTAATTTATG TAAACCTGGA 
    AACTCTGGGA TTCAGTCTCT AATTGGAGTA CTTTGCATTC CAAATATGGA AATAAGGCGA
    GGTCTCCTTG AAGTGCTTTA TGATATATTT CGTCTTCCTC TACCTGTTGT GACTGAAGAG
    TTCATAGAAG CACTACTCAG TGTAGATCCA GGTAGATTCC AAGACAGTTG GAGGCTTTCA
    GATGGCTTTG TAGCAGCTGA GGCAAAAACT ATTCTTCCTC ATCGTGCCAG ATCCAGGCCA
    GACCTCATGG ATAATTATTT GGCACTGATA CTCTCAGCAT TTATTCGTAA TGGACTTTTA
    GAAGGTTTGG TTGAGGTAAT AACAAACAGT GACGACCATA TCTCAGTTAG AGCCACCATC
    CTTTTAGGAG AACTTTTACA TATGGCAAAC ACAATTCTTC CTCATTCACA TAGCCATCAT
    TTGCACTGCC TGCCAACCCT AATGAATATG GCTGCATCCT TTGATATCCC TAAGGAAAAG
    AGATTGCGAG CCAGTGCAGC TTTGAACTGT TTAAAACGCT TCCATGAAAT GAAAAAACGA
    GGACCTAAAC CTTATAGCCT TCATTTAGAT CACATTATTC AGAAAGCAAT TGCAACACAT
    CAGAAACGGG ATCAGTATCT CCGAGCTCAG AAAGATATAT TTGTTCTTAA GGATACAGAG
    GAAGCTCTTC TAATGAACCT TAGAGATAGC CAAGTCCTTC AACATAAAGA GAATCTTGAA
    TGGAATTGGA ATCTTATTGG GACCATTCTT AAGTGGCCAA ATGTAAATCT AAGAAACTAT
    AAAGATGAGC AGTTGCACAG GTTTGTACGA AGACTACTTT ATTTTTACAA GCCCAGTAGT
    AAGTTATACG CCAATCTGGA TCTGGATTTT GCCAAGTCCA AGCAGCTCAC AGTTGTGGGT
    TGTCAGTTTA CAGAATTTCT TCTTGAGTCT GAAGAGGATG GGCAGGGATA CTTAGAAGAT
    CTAGTAAAGG ATATTGTTCA GTGGCTCAAT GCCTCTTCTG GAATGAAACC TGAAAGAAGC
    CTTCAAAATA ATGGTTTATT GACTACTCTT AGTCAACACT ACTTTTTATT TATTGGAACA
    CTTTCTTGCC ACCCTCATGG CGTCAAAATG CTGGAGAAAT GCAGCGTATT TCAATGTTTA
    CTTAACCTTT GCTCCTTGAA AAACCAAGAT CACTTGCTAA AACTGACCGT TTCAAGCTTG
    GACTATAGTA GAGATGGTTT GGCCAGGGTC ATCCTTTCCA AAATCTTAAC AGCAGCCACT
    GATGCCTGCA GACTGTATGC AACAAAACAT TTAAGAGTGT TATTGAGAGC TAATGTTGAA
    TTCTTCAATA ATTGGGGAAT TGAATTACTA GTGACCCAGC TGCATGATAA AAACAAAACG
    ATTTCTTCTG AGGCCCTTGA TATTCTCGAT GAAGCATGTG AAGACAAGGC CAATCTTCAT
    GCACTTATTC AGATGAAGCC AGCTTTATCT CACCTTGGGG ACAAGGGTTT GCTTCTCCTC
    CTGAGATTTC TCTCCATTCC AAAAGGGTTT TCCTATTTGA ATGAAAGGGG TTATGTAACA
    AAACAGTTGG AAAAGTGGCA CAAGGAATAT AATTCAAAAT ACGTTGACTT GATTGAAGAA
    CAGCTTAATG AAGCACTTAC CACTTACCGG AAGCCAATTG ATGGTGACAA CTATGTTCGT
    CGGAGTAACC AAAGATTACA GCGTCCTCAT GTCTACCTAC CTGTACATCT TTATGGACAA
    CTGGTACACC ACAAAACAGG CTGTCATTTA TTGGAAGTAC AGAATATTAT TACAGAACTC
    TGTCACAATG TTCGTACACC AGATTTGGAT AAATGGGAAG AAATTAAAAA ACTAAAAGCA
    TCTCTTTGGG CCTTGGGAAA TATTGGCTCA TCTAATTGGG GTCTCAATTT GCTACAAGAA
    GAAAACGTGA TTCCAGATAT CCTGAAACTT GCAAAACAGT GTGAAGTTCT TTCAGTCAGA
    GGGACCTGTG TATATGTTCT TGGGCTCATA GCCAAAACCA AGCAAGGCTG TGATATTCTA
    AAATGTCACA ATTGGGATGC TGTAAGGCAC AGTCGTAAAC ATTTGTGGCC AGTGGTTCCA
    GACGATGTAG AACAACTCTG TAATGAACTC TCATCTGTCC CGAGCACCCT AAGTTTGAAC
    TCGGAGTCAA CAAGCTCTAG GCATAATAGT GAGAGTGAAT CTGCACCGTC AAGTATGTTC
    ATCATGGAGG ATGACCGGTT TGGCAGCAGC TCTACCAGTA CATTTTTCCT TGACATCAAT
    GAAGATGCAG AGCCAGCATT TTATGATCGA CCTGGACCTA TAAAGGATAA AAATCCATTC
    CCTTTCTTTG CTTCTAGTAA ACTTGTGAAG AATCGTATCT TAAATTCTCT TACTTTGCCT
    AATAAAAAAC ATCGTAGCAG CAGTGATCCA AAAGGAGGGA AACTGTCATC TGAAAATAAG
    ACAAGCAACA GGCGGATCAG AACGCTTACA GAGCCCAGTG TTGACTTTAA TCATAGTGAT
    GATTTCACAC CCATATCCAC AGTACAGAAA ACATTACAAT TAGAAACTTC ATTTGTTGGG
    AATAAGCACA TTGAAGACAC TGGTAGTACT CCAAGTATTG GAGAGAATGA CTTAAAATTC
    ACCAAGAGTC TTGGTACAGA GAATCACAAA GAAAACACAA GCCGAGAGAG ATTAGTTGTA
    GAAAGTTCAT CGAGCTCACA TATGAAGATA CGTAGCCAGA GTTTTAATAC AGATACTACA
    ACAAGTGGCA TAAGTTCAAT GAGTTCAAGT CCTTCACGAG AGACAGTAGG TGTAGATGCT
    ACGACTGTGG ACACAGACTG TGGAAGTATG AGTACTGTGG TAAGTACTAA AACTGTTAAG
    ACAAGCCACT ATTTGACGCC ACAGTCTAAC CATCTCTCTC TCTCTAAATC AAGCTCAGTG
    TCCCTGGTGC CTCCAGGTTC TTCTCACACA CTTCCTAGAA GAGCACAGTC CCTTAAAGCA
    CCCTCTATTG CTACAATTAA AAGTCTAGCA GACTATAACT TTAGTTACAC AAGTTCTAGA
    GATGCCTTTG GCTACGCTAC CCTGAAAAGA TTACAACAGC AAAGAATGCA TCCATCCTTA
    TCTCATTCTG AAGCTTTGGC ATCCCCAGCA AAAGATGTGC TGTTTACTGA TACCATCACC
    ATGAAGGCCA ACAGCTTTGA GTCCAGGTTA ACACCAAGCA GGATCAATTT TAAAAAGAAG
    CATGTCGGGG GAATCAGGAG CTTAAGACCT ACAATAACAA ACAACTTTTT CAGGTTCATG
    AAAGCTTTAA GTTATGCTTC GCTAGATAAA GAAGATTTAT TAAGTCCTAT TAACCAAAAT
    ACGCTGCAGC GCTCCTCCTC CGTGAGGTCC ATGGTGTCCA GTGCGACATA CGGTAGCTCA
    GATGACTATA TTGGTCTTGC TCTTCCAGTA GATATCAATG ATATATTCCA GGTAAAGGAT
    ATTCCTTATT TTCAAACAAA AAACATACCT CCACATGATG ATCGAGGTGC AAGAGTATTT
    GCCCATGATG TTGGAGGTCT TCCATCTGGA ACTGGAGGTC TTGTAAAAAA CTCTTTTCAC
    TTGCTACGAC AGCAGATGAG TCTTACGGAA ATAATGAATT CAATCCATTC AGATGCTTCT
    CTGTTTTTAG AAAGTACAGA AGACACTGGA CTACAGGAAC ATACAGATGA TAACTGCCTT
    TATTGTGTCT GTATTGAAAT TCTGGGTTTC CAGCCCAGTA ACCAACTGAG TGCAATATGT
    AGTCATTCAG ACCTTCAAGA CATTCCATAT TCTGATTGGT GTGAGCAGAC TATCCATAAT
    CCCTTAGAAG TCGTTCCCTC TAAATTTTCT GGCATTTCTG GATGCAGTGA TGGAGTGTCT
    CAAGAAGGCT CAGCCAGCAG CACCAAAAGT ACAGAATTGC TACTAGGTGT TAAAACAATT
    CCAGATGATA CACCAATGTG CCGTATACTC CTTCGTAAAG AAGTTCTGAG ATTAGTCATT
    AATTTGAGTA GTTCAGTTTC AACTAAATGT CATGAAACTG GGCTTTTAAC AATTAAAGAG
    AAGTATCCTC AAACATTTGA TGACATATGC CTTTACTCTG AGGTTTCCCA TTTGCTGTCA
    CACTGCACAT TTAGACTTCC ATGTCGGAGG TTCATACAAG AATTATTTCA GGATGTACAG
    TTTTTACAAA TGCATGAAGA AGCAGAGGCT GTCTTGGCAA CACCACCAAA GCAAACTATA
    GTCGATACAT CTGCTGAATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837067.1
    CDS168..4511
    Translation

    Target ORF information:

    RefSeq Version XM_024981299.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGAATCA TAGCAACATT CCGGTCATGG GCAGGTATTA TTAATTTATG TAAACCTGGA 
    AACTCTGGGA TTCAGTCTCT AATTGGAGTA CTTTGCATTC CAAATATGGA AATAAGGCGA
    GGTCTCCTTG AAGTGCTTTA TGATATATTT CGTCTTCCTC TACCTGTTGT GACTGAAGAG
    TTCATAGAAG CACTACTCAG TGTAGATCCA GGTAGATTCC AAGACAGTTG GAGGCTTTCA
    GATGGCTTTG TAGCAGCTGA GGCAAAAACT ATTCTTCCTC ATCGTGCCAG ATCCAGGCCA
    GACCTCATGG ATAATTATTT GGCACTGATA CTCTCAGCAT TTATTCGTAA TGGACTTTTA
    GAAGGTTTGG TTGAGGTAAT AACAAACAGT GACGACCATA TCTCAGTTAG AGCCACCATC
    CTTTTAGGAG AACTTTTACA TATGGCAAAC ACAATTCTTC CTCATTCACA TAGCCATCAT
    TTGCACTGCC TGCCAACCCT AATGAATATG GCTGCATCCT TTGATATCCC TAAGGAAAAG
    AGATTGCGAG CCAGTGCAGC TTTGAACTGT TTAAAACGCT TCCATGAAAT GAAAAAACGA
    GGACCTAAAC CTTATAGCCT TCATTTAGAT CACATTATTC AGAAAGCAAT TGCAACACAT
    CAGAAACGGG ATCAGTATCT CCGAGCTCAG AAAGATATAT TTGTTCTTAA GGATACAGAG
    GAAGCTCTTC TAATGAACCT TAGAGATAGC CAAGTCCTTC AACATAAAGA GAATCTTGAA
    TGGAATTGGA ATCTTATTGG GACCATTCTT AAGTGGCCAA ATGTAAATCT AAGAAACTAT
    AAAGATGAGC AGTTGCACAG GTTTGTACGA AGACTACTTT ATTTTTACAA GCCCAGTAGT
    AAGTTATACG CCAATCTGGA TCTGGATTTT GCCAAGTCCA AGCAGCTCAC AGTTGTGGGT
    TGTCAGTTTA CAGAATTTCT TCTTGAGTCT GAAGAGGATG GGCAGGGATA CTTAGAAGAT
    CTAGTAAAGG ATATTGTTCA GTGGCTCAAT GCCTCTTCTG GAATGAAACC TGAAAGAAGC
    CTTCAAAATA ATGGTTTATT GACTACTCTT AGTCAACACT ACTTTTTATT TATTGGAACA
    CTTTCTTGCC ACCCTCATGG CGTCAAAATG CTGGAGAAAT GCAGCGTATT TCAATGTTTA
    CTTAACCTTT GCTCCTTGAA AAACCAAGAT CACTTGCTAA AACTGACCGT TTCAAGCTTG
    GACTATAGTA GAGATGGTTT GGCCAGGGTC ATCCTTTCCA AAATCTTAAC AGCAGCCACT
    GATGCCTGCA GACTGTATGC AACAAAACAT TTAAGAGTGT TATTGAGAGC TAATGTTGAA
    TTCTTCAATA ATTGGGGAAT TGAATTACTA GTGACCCAGC TGCATGATAA AAACAAAACG
    ATTTCTTCTG AGGCCCTTGA TATTCTCGAT GAAGCATGTG AAGACAAGGC CAATCTTCAT
    GCACTTATTC AGATGAAGCC AGCTTTATCT CACCTTGGGG ACAAGGGTTT GCTTCTCCTC
    CTGAGATTTC TCTCCATTCC AAAAGGGTTT TCCTATTTGA ATGAAAGGGG TTATGTAACA
    AAACAGTTGG AAAAGTGGCA CAAGGAATAT AATTCAAAAT ACGTTGACTT GATTGAAGAA
    CAGCTTAATG AAGCACTTAC CACTTACCGG AAGCCAATTG ATGGTGACAA CTATGTTCGT
    CGGAGTAACC AAAGATTACA GCGTCCTCAT GTCTACCTAC CTGTACATCT TTATGGACAA
    CTGGTACACC ACAAAACAGG CTGTCATTTA TTGGAAGTAC AGAATATTAT TACAGAACTC
    TGTCACAATG TTCGTACACC AGATTTGGAT AAATGGGAAG AAATTAAAAA ACTAAAAGCA
    TCTCTTTGGG CCTTGGGAAA TATTGGCTCA TCTAATTGGG GTCTCAATTT GCTACAAGAA
    GAAAACGTGA TTCCAGATAT CCTGAAACTT GCAAAACAGT GTGAAGTTCT TTCAGTCAGA
    GGGACCTGTG TATATGTTCT TGGGCTCATA GCCAAAACCA AGCAAGGCTG TGATATTCTA
    AAATGTCACA ATTGGGATGC TGTAAGGCAC AGTCGTAAAC ATTTGTGGCC AGTGGTTCCA
    GACGATGTAG AACAACTCTG TAATGAACTC TCATCTGTCC CGAGCACCCT AAGTTTGAAC
    TCGGAGTCAA CAAGCTCTAG GCATAATAGT GAGAGTGAAT CTGCACCGTC AAGTATGTTC
    ATCATGGAGG ATGACCGGTT TGGCAGCAGC TCTACCAGTA CATTTTTCCT TGACATCAAT
    GAAGATGCAG AGCCAGCATT TTATGATCGA CCTGGACCTA TAAAGGATAA AAATCCATTC
    CCTTTCTTTG CTTCTAGTAA ACTTGTGAAG AATCGTATCT TAAATTCTCT TACTTTGCCT
    AATAAAAAAC ATCGTAGCAG CAGTGATCCA AAAGGAGGGA AACTGTCATC TGAAAATAAG
    ACAAGCAACA GGCGGATCAG AACGCTTACA GAGCCCAGTG TTGACTTTAA TCATAGTGAT
    GATTTCACAC CCATATCCAC AGTACAGAAA ACATTACAAT TAGAAACTTC ATTTGTTGGG
    AATAAGCACA TTGAAGACAC TGGTAGTACT CCAAGTATTG GAGAGAATGA CTTAAAATTC
    ACCAAGAGTC TTGGTACAGA GAATCACAAA GAAAACACAA GCCGAGAGAG ATTAGTTGTA
    GAAAGTTCAT CGAGCTCACA TATGAAGATA CGTAGCCAGA GTTTTAATAC AGATACTACA
    ACAAGTGGCA TAAGTTCAAT GAGTTCAAGT CCTTCACGAG AGACAGTAGG TGTAGATGCT
    ACGACTGTGG ACACAGACTG TGGAAGTATG AGTACTGTGG TAAGTACTAA AACTGTTAAG
    ACAAGCCACT ATTTGACGCC ACAGTCTAAC CATCTCTCTC TCTCTAAATC AAGCTCAGTG
    TCCCTGGTGC CTCCAGGTTC TTCTCACACA CTTCCTAGAA GAGCACAGTC CCTTAAAGCA
    CCCTCTATTG CTACAATTAA AAGTCTAGCA GACTATAACT TTAGTTACAC AAGTTCTAGA
    GATGCCTTTG GCTACGCTAC CCTGAAAAGA TTACAACAGC AAAGAATGCA TCCATCCTTA
    TCTCATTCTG AAGCTTTGGC ATCCCCAGCA AAAGATGTGC TGTTTACTGA TACCATCACC
    ATGAAGGCCA ACAGCTTTGA GTCCAGGTTA ACACCAAGCA GGATCAATTT TAAAAAGAAG
    CATGTCGGGG GAATCAGGAG CTTAAGACCT ACAATAACAA ACAACTTTTT CAGGTTCATG
    AAAGCTTTAA GTTATGCTTC GCTAGATAAA GAAGATTTAT TAAGTCCTAT TAACCAAAAT
    ACGCTGCAGC GCTCCTCCTC CGTGAGGTCC ATGGTGTCCA GTGCGACATA CGGTAGCTCA
    GATGACTATA TTGGTCTTGC TCTTCCAGTA GATATCAATG ATATATTCCA GGTAAAGGAT
    ATTCCTTATT TTCAAACAAA AAACATACCT CCACATGATG ATCGAGGTGC AAGAGTATTT
    GCCCATGATG TTGGAGGTCT TCCATCTGGA ACTGGAGGTC TTGTAAAAAA CTCTTTTCAC
    TTGCTACGAC AGCAGATGAG TCTTACGGAA ATAATGAATT CAATCCATTC AGATGCTTCT
    CTGTTTTTAG AAAGTACAGA AGACACTGGA CTACAGGAAC ATACAGATGA TAACTGCCTT
    TATTGTGTCT GTATTGAAAT TCTGGGTTTC CAGCCCAGTA ACCAACTGAG TGCAATATGT
    AGTCATTCAG ACCTTCAAGA CATTCCATAT TCTGATTGGT GTGAGCAGAC TATCCATAAT
    CCCTTAGAAG TCGTTCCCTC TAAATTTTCT GGCATTTCTG GATGCAGTGA TGGAGTGTCT
    CAAGAAGGCT CAGCCAGCAG CACCAAAAGT ACAGAATTGC TACTAGGTGT TAAAACAATT
    CCAGATGATA CACCAATGTG CCGTATACTC CTTCGTAAAG AAGTTCTGAG ATTAGTCATT
    AATTTGAGTA GTTCAGTTTC AACTAAATGT CATGAAACTG GGCTTTTAAC AATTAAAGAG
    AAGTATCCTC AAACATTTGA TGACATATGC CTTTACTCTG AGGTTTCCCA TTTGCTGTCA
    CACTGCACAT TTAGACTTCC ATGTCGGAGG TTCATACAAG AATTATTTCA GGATGTACAG
    TTTTTACAAA TGCATGAAGA AGCAGAGGCT GTCTTGGCAA CACCACCAAA GCAAACTATA
    GTCGATACAT CTGCTGAATC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228022
    Clone ID Related Accession (Same CDS sequence) XM_024981297.1
    Accession Version XM_024981297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGGGA
    AATATTGGCT CATCTAATTG GGGTCTCAAT TTGCTACAAG AAGAAAACGT GATTCCAGAT
    ATCCTGAAAC TTGCAAAACA GTGTGAAGTT CTTTCAGTCA GAGGGACCTG TGTATATGTT
    CTTGGGCTCA TAGCCAAAAC CAAGCAAGGC TGTGATATTC TAAAATGTCA CAATTGGGAT
    GCTGTAAGGC ACAGTCGTAA ACATTTGTGG CCAGTGGTTC CAGACGATGT AGAACAACTC
    TGTAATGAAC TCTCATCTGT CCCGAGCACC CTAAGTTTGA ACTCGGAGTC AACAAGCTCT
    AGGCATAATA GTGAGAGTGA ATCTGCACCG TCAAGTATGT TCATCATGGA GGATGACCGG
    TTTGGCAGCA GCTCTACCAG TACATTTTTC CTTGACATCA ATGAAGATGC AGAGCCAGCA
    TTTTATGATC GACCTGGACC TATAAAGGAT AAAAATCCAT TCCCTTTCTT TGCTTCTAGT
    AAACTTGTGA AGAATCGTAT CTTAAATTCT CTTACTTTGC CTAATAAAAA ACATCGTAGC
    AGCAGTGATC CAAAAGGAGG GAAACTGTCA TCTGAAAATA AGACAAGCAA CAGGCGGATC
    AGAACGCTTA CAGAGCCCAG TGTTGACTTT AATCATAGTG ATGATTTCAC ACCCATATCC
    ACAGTACAGA AAACATTACA ATTAGAAACT TCATTTGTTG GGAATAAGCA CATTGAAGAC
    ACTGGTAGTA CTCCAAGTAT TGGAGAGAAT GACTTAAAAT TCACCAAGAG TCTTGGTACA
    GAGAATCACA AAGAAAACAC AAGCCGAGAG AGATTAGTTG TAGAAAGTTC ATCGAGCTCA
    CATATGAAGA TACGTAGCCA GAGTTTTAAT ACAGATACTA CAACAAGTGG CATAAGTTCA
    ATGAGTTCAA GTCCTTCACG AGAGACAGTA GGTGTAGATG CTACGACTGT GGACACAGAC
    TGTGGAAGTA TGAGTACTGT GGTAAGTACT AAAACTGTTA AGACAAGCCA CTATTTGACG
    CCACAGTCTA ACCATCTCTC TCTCTCTAAA TCAAGCTCAG TGTCCCTGGT GCCTCCAGGT
    TCTTCTCACA CACTTCCTAG AAGAGCACAG TCCCTTAAAG CACCCTCTAT TGCTACAATT
    AAAAGTCTAG CAGACTATAA CTTTAGTTAC ACAAGTTCTA GAGATGCCTT TGGCTACGCT
    ACCCTGAAAA GATTACAACA GCAAAGAATG CATCCATCCT TATCTCATTC TGAAGCTTTG
    GCATCCCCAG CAAAAGATGT GCTGTTTACT GATACCATCA CCATGAAGGC CAACAGCTTT
    GAGTCCAGGT TAACACCAAG CAGGATCAAT TTTAAAAAGA AGCATGTCGG GGGAATCAGG
    AGCTTAAGAC CTACAATAAC AAACAACTTT TTCAGGTTCA TGAAAGCTTT AAGTTATGCT
    TCGCTAGATA AAGAAGATTT ATTAAGTCCT ATTAACCAAA ATACGCTGCA GCGCTCCTCC
    TCCGTGAGGT CCATGGTGTC CAGTGCGACA TACGGTAGCT CAGATGACTA TATTGGTCTT
    GCTCTTCCAG TAGATATCAA TGATATATTC CAGGTAAAGG ATATTCCTTA TTTTCAAACA
    AAAAACATAC CTCCACATGA TGATCGAGGT GCAAGAGTAT TTGCCCATGA TGTTGGAGGT
    CTTCCATCTG GAACTGGAGG TCTTGTAAAA AACTCTTTTC ACTTGCTACG ACAGCAGATG
    AGTCTTACGG AAATAATGAA TTCAATCCAT TCAGATGCTT CTCTGTTTTT AGAAAGTACA
    GAAGACACTG GACTACAGGA ACATACAGAT GATAACTGCC TTTATTGTGT CTGTATTGAA
    ATTCTGGGTT TCCAGCCCAG TAACCAACTG AGTGCAATAT GTAGTCATTC AGACCTTCAA
    GACATTCCAT ATTCTGATTG GTGTGAGCAG ACTATCCATA ATCCCTTAGA AGTCGTTCCC
    TCTAAATTTT CTGGCATTTC TGGATGCAGT GATGGAGTGT CTCAAGAAGG CTCAGCCAGC
    AGCACCAAAA GTACAGAATT GCTACTAGGT GTTAAAACAA TTCCAGATGA TACACCAATG
    TGCCGTATAC TCCTTCGTAA AGAAGTTCTG AGATTAGTCA TTAATTTGAG TAGTTCAGTT
    TCAACTAAAT GTCATGAAAC TGGGCTTTTA ACAATTAAAG AGAAGTATCC TCAAACATTT
    GATGACATAT GCCTTTACTC TGAGGTTTCC CATTTGCTGT CACACTGCAC ATTTAGACTT
    CCATGTCGGA GGTTCATACA AGAATTATTT CAGGATGTAC AGTTTTTACA AATGCATGAA
    GAAGCAGAGG CTGTCTTGGC AACACCACCA AAGCAAACTA TAGTCGATAC ATCTGCTGAA
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837065.1
    CDS107..5212
    Translation

    Target ORF information:

    RefSeq Version XM_024981297.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGGGA
    AATATTGGCT CATCTAATTG GGGTCTCAAT TTGCTACAAG AAGAAAACGT GATTCCAGAT
    ATCCTGAAAC TTGCAAAACA GTGTGAAGTT CTTTCAGTCA GAGGGACCTG TGTATATGTT
    CTTGGGCTCA TAGCCAAAAC CAAGCAAGGC TGTGATATTC TAAAATGTCA CAATTGGGAT
    GCTGTAAGGC ACAGTCGTAA ACATTTGTGG CCAGTGGTTC CAGACGATGT AGAACAACTC
    TGTAATGAAC TCTCATCTGT CCCGAGCACC CTAAGTTTGA ACTCGGAGTC AACAAGCTCT
    AGGCATAATA GTGAGAGTGA ATCTGCACCG TCAAGTATGT TCATCATGGA GGATGACCGG
    TTTGGCAGCA GCTCTACCAG TACATTTTTC CTTGACATCA ATGAAGATGC AGAGCCAGCA
    TTTTATGATC GACCTGGACC TATAAAGGAT AAAAATCCAT TCCCTTTCTT TGCTTCTAGT
    AAACTTGTGA AGAATCGTAT CTTAAATTCT CTTACTTTGC CTAATAAAAA ACATCGTAGC
    AGCAGTGATC CAAAAGGAGG GAAACTGTCA TCTGAAAATA AGACAAGCAA CAGGCGGATC
    AGAACGCTTA CAGAGCCCAG TGTTGACTTT AATCATAGTG ATGATTTCAC ACCCATATCC
    ACAGTACAGA AAACATTACA ATTAGAAACT TCATTTGTTG GGAATAAGCA CATTGAAGAC
    ACTGGTAGTA CTCCAAGTAT TGGAGAGAAT GACTTAAAAT TCACCAAGAG TCTTGGTACA
    GAGAATCACA AAGAAAACAC AAGCCGAGAG AGATTAGTTG TAGAAAGTTC ATCGAGCTCA
    CATATGAAGA TACGTAGCCA GAGTTTTAAT ACAGATACTA CAACAAGTGG CATAAGTTCA
    ATGAGTTCAA GTCCTTCACG AGAGACAGTA GGTGTAGATG CTACGACTGT GGACACAGAC
    TGTGGAAGTA TGAGTACTGT GGTAAGTACT AAAACTGTTA AGACAAGCCA CTATTTGACG
    CCACAGTCTA ACCATCTCTC TCTCTCTAAA TCAAGCTCAG TGTCCCTGGT GCCTCCAGGT
    TCTTCTCACA CACTTCCTAG AAGAGCACAG TCCCTTAAAG CACCCTCTAT TGCTACAATT
    AAAAGTCTAG CAGACTATAA CTTTAGTTAC ACAAGTTCTA GAGATGCCTT TGGCTACGCT
    ACCCTGAAAA GATTACAACA GCAAAGAATG CATCCATCCT TATCTCATTC TGAAGCTTTG
    GCATCCCCAG CAAAAGATGT GCTGTTTACT GATACCATCA CCATGAAGGC CAACAGCTTT
    GAGTCCAGGT TAACACCAAG CAGGATCAAT TTTAAAAAGA AGCATGTCGG GGGAATCAGG
    AGCTTAAGAC CTACAATAAC AAACAACTTT TTCAGGTTCA TGAAAGCTTT AAGTTATGCT
    TCGCTAGATA AAGAAGATTT ATTAAGTCCT ATTAACCAAA ATACGCTGCA GCGCTCCTCC
    TCCGTGAGGT CCATGGTGTC CAGTGCGACA TACGGTAGCT CAGATGACTA TATTGGTCTT
    GCTCTTCCAG TAGATATCAA TGATATATTC CAGGTAAAGG ATATTCCTTA TTTTCAAACA
    AAAAACATAC CTCCACATGA TGATCGAGGT GCAAGAGTAT TTGCCCATGA TGTTGGAGGT
    CTTCCATCTG GAACTGGAGG TCTTGTAAAA AACTCTTTTC ACTTGCTACG ACAGCAGATG
    AGTCTTACGG AAATAATGAA TTCAATCCAT TCAGATGCTT CTCTGTTTTT AGAAAGTACA
    GAAGACACTG GACTACAGGA ACATACAGAT GATAACTGCC TTTATTGTGT CTGTATTGAA
    ATTCTGGGTT TCCAGCCCAG TAACCAACTG AGTGCAATAT GTAGTCATTC AGACCTTCAA
    GACATTCCAT ATTCTGATTG GTGTGAGCAG ACTATCCATA ATCCCTTAGA AGTCGTTCCC
    TCTAAATTTT CTGGCATTTC TGGATGCAGT GATGGAGTGT CTCAAGAAGG CTCAGCCAGC
    AGCACCAAAA GTACAGAATT GCTACTAGGT GTTAAAACAA TTCCAGATGA TACACCAATG
    TGCCGTATAC TCCTTCGTAA AGAAGTTCTG AGATTAGTCA TTAATTTGAG TAGTTCAGTT
    TCAACTAAAT GTCATGAAAC TGGGCTTTTA ACAATTAAAG AGAAGTATCC TCAAACATTT
    GATGACATAT GCCTTTACTC TGAGGTTTCC CATTTGCTGT CACACTGCAC ATTTAGACTT
    CCATGTCGGA GGTTCATACA AGAATTATTT CAGGATGTAC AGTTTTTACA AATGCATGAA
    GAAGCAGAGG CTGTCTTGGC AACACCACCA AAGCAAACTA TAGTCGATAC ATCTGCTGAA
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36073
    Clone ID Related Accession (Same CDS sequence) NM_001144096.3
    Accession Version NM_001144096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-24
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000020.1. On Feb 22, 2016 this sequence version replaced NM_001144096.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.50478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145447, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGTTC
    ATGAAAGCTT TAAGTTATGC TTCGCTAGAT AAAGAAGATT TATTAAGTCC TATTAACCAA
    AATACGCTGC AGCGCTCCTC CTCCGTGAGG TCCATGGTGT CCAGTGCGAC ATACGGTAGC
    TCAGATGACT ATATTGGTCT TGCTCTTCCA GTAGATATCA ATGATATATT CCAGGTAAAG
    GATATTCCTT ATTTTCAAAC AAAAAACATA CCTCCACATG ATGATCGAGG TGCAAGAGTA
    TTTGCCCATG ATGTTGGAGG TCTTCCATCT GGAACTGGAG GTCTTGTAAA AAACTCTTTT
    CACTTGCTAC GACAGCAGAT GAGTCTTACG GAAATAATGA ATTCAATCCA TTCAGATGCT
    TCTCTGTTTT TAGAAAGTAC AGAAGACACT GGACTACAGG AACATACAGA TGATAACTGC
    CTTTATTGTG TCTGTATTGA AATTCTGGGT TTCCAGCCCA GTAACCAACT GAGTGCAATA
    TGTAGTCATT CAGACCTTCA AGACATTCCA TATTCTGATT GGTGTGAGCA GACTATCCAT
    AATCCCTTAG AAGTCGTTCC CTCTAAATTT TCTGGCATTT CTGGATGCAG TGATGGAGTG
    TCTCAAGAAG GCTCAGCCAG CAGCACCAAA AGTACAGAAT TGCTACTAGG TGTTAAAACA
    ATTCCAGATG ATACACCAAT GTGCCGTATA CTCCTTCGTA AAGAAGTTCT GAGATTAGTC
    ATTAATTTGA GTAGTTCAGT TTCAACTAAA TGTCATGAAA CTGGGCTTTT AACAATTAAA
    GAGAAGTATC CTCAAACATT TGATGACATA TGCCTTTACT CTGAGGTTTC CCATTTGCTG
    TCACACTGCA CATTTAGACT TCCATGTCGG AGGTTCATAC AAGAATTATT TCAGGATGTA
    CAGTTTTTAC AAATGCATGA AGAAGCAGAG GCTGTCTTGG CAACACCACC AAAGCAAACT
    ATAGTCGATA CATCTGCTGA ATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137568.1
    CDS25..5151
    Translation

    Target ORF information:

    RefSeq Version NM_001144096.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGTTC
    ATGAAAGCTT TAAGTTATGC TTCGCTAGAT AAAGAAGATT TATTAAGTCC TATTAACCAA
    AATACGCTGC AGCGCTCCTC CTCCGTGAGG TCCATGGTGT CCAGTGCGAC ATACGGTAGC
    TCAGATGACT ATATTGGTCT TGCTCTTCCA GTAGATATCA ATGATATATT CCAGGTAAAG
    GATATTCCTT ATTTTCAAAC AAAAAACATA CCTCCACATG ATGATCGAGG TGCAAGAGTA
    TTTGCCCATG ATGTTGGAGG TCTTCCATCT GGAACTGGAG GTCTTGTAAA AAACTCTTTT
    CACTTGCTAC GACAGCAGAT GAGTCTTACG GAAATAATGA ATTCAATCCA TTCAGATGCT
    TCTCTGTTTT TAGAAAGTAC AGAAGACACT GGACTACAGG AACATACAGA TGATAACTGC
    CTTTATTGTG TCTGTATTGA AATTCTGGGT TTCCAGCCCA GTAACCAACT GAGTGCAATA
    TGTAGTCATT CAGACCTTCA AGACATTCCA TATTCTGATT GGTGTGAGCA GACTATCCAT
    AATCCCTTAG AAGTCGTTCC CTCTAAATTT TCTGGCATTT CTGGATGCAG TGATGGAGTG
    TCTCAAGAAG GCTCAGCCAG CAGCACCAAA AGTACAGAAT TGCTACTAGG TGTTAAAACA
    ATTCCAGATG ATACACCAAT GTGCCGTATA CTCCTTCGTA AAGAAGTTCT GAGATTAGTC
    ATTAATTTGA GTAGTTCAGT TTCAACTAAA TGTCATGAAA CTGGGCTTTT AACAATTAAA
    GAGAAGTATC CTCAAACATT TGATGACATA TGCCTTTACT CTGAGGTTTC CCATTTGCTG
    TCACACTGCA CATTTAGACT TCCATGTCGG AGGTTCATAC AAGAATTATT TCAGGATGTA
    CAGTTTTTAC AAATGCATGA AGAAGCAGAG GCTGTCTTGG CAACACCACC AAAGCAAACT
    ATAGTCGATA CATCTGCTGA ATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30669
    Clone ID Related Accession (Same CDS sequence) XM_015459092.1 , XM_024981298.1
    Accession Version XM_015459092.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGAAAGC TAGGCCATCT GAATAACTTT ACTAAGCTGC TTTGTGATAT TGGCCACAGT 
    GAAGAAAAAC TGGGTTTTAA CTATGAGGAT ATCATAATTT GCTTGCGGTT AGCTTTATTG
    AATGAAGCAA AAGAAGTGCG GGCAGCAGGG CTTCGAGCTC TTCGGTATCT CATCCAAGAC
    TCCAGTATTC TGCAGAAGGT GCTAAAATTG AAAGTGGACT ATTTAATAGC TAGGTGCATT
    GACATACAGC AGAGCAACGA GGTAGAGAGG ACACAAGCAC TTCGATTAGT CAGAAAGATG
    ATTACTGTGA ATGCTTCCTT GTTTCCTAGC TCTGTGGCCA ACTCATTAAT AGCAGTTGGA
    AATGATGGAC TTCAAGAAAG AGACAGAATG GTTCGAGCAT GCATTGCCAT TATCTGTGAA
    CTAGCACTTC AGAATCCAGA GGTGGTTGCC CTTCGAGGTG GACTGAACAC CATCTTGAAA
    AATGTGATTG ATTGCCAATT AAGTCGAATA AATGAGGCTC TAATTACTAC AATTTTGCAC
    CTTCTTAATC ATCCAAAGAC CCGACAGTAT GTACGAGCTG ATGTCGAATT AGAGCGAATT
    TTAGCACCCT ATACCGATTT TCACTACAGA CATAGTCCAG ATACAGCTGA AGGACAGCTA
    AAAGAAGACA GAGAAGCAAG GTTTCTAGCC AGTAAAATGG GAATCATAGC AACATTCCGG
    TCATGGGCAG GTATTATTAA TTTATGTAAA CCTGGAAACT CTGGGATTCA GTCTCTAATT
    GGAGTACTTT GCATTCCAAA TATGGAAATA AGGCGAGGTC TCCTTGAAGT GCTTTATGAT
    ATATTTCGTC TTCCTCTACC TGTTGTGACT GAAGAGTTCA TAGAAGCACT ACTCAGTGTA
    GATCCAGGTA GATTCCAAGA CAGTTGGAGG CTTTCAGATG GCTTTGTAGC AGCTGAGGCA
    AAAACTATTC TTCCTCATCG TGCCAGATCC AGGCCAGACC TCATGGATAA TTATTTGGCA
    CTGATACTCT CAGCATTTAT TCGTAATGGA CTTTTAGAAG GTTTGGTTGA GGTAATAACA
    AACAGTGACG ACCATATCTC AGTTAGAGCC ACCATCCTTT TAGGAGAACT TTTACATATG
    GCAAACACAA TTCTTCCTCA TTCACATAGC CATCATTTGC ACTGCCTGCC AACCCTAATG
    AATATGGCTG CATCCTTTGA TATCCCTAAG GAAAAGAGAT TGCGAGCCAG TGCAGCTTTG
    AACTGTTTAA AACGCTTCCA TGAAATGAAA AAACGAGGAC CTAAACCTTA TAGCCTTCAT
    TTAGATCACA TTATTCAGAA AGCAATTGCA ACACATCAGA AACGGGATCA GTATCTCCGA
    GCTCAGAAAG ATATATTTGT TCTTAAGGAT ACAGAGGAAG CTCTTCTAAT GAACCTTAGA
    GATAGCCAAG TCCTTCAACA TAAAGAGAAT CTTGAATGGA ATTGGAATCT TATTGGGACC
    ATTCTTAAGT GGCCAAATGT AAATCTAAGA AACTATAAAG ATGAGCAGTT GCACAGGTTT
    GTACGAAGAC TACTTTATTT TTACAAGCCC AGTAGTAAGT TATACGCCAA TCTGGATCTG
    GATTTTGCCA AGTCCAAGCA GCTCACAGTT GTGGGTTGTC AGTTTACAGA ATTTCTTCTT
    GAGTCTGAAG AGGATGGGCA GGGATACTTA GAAGATCTAG TAAAGGATAT TGTTCAGTGG
    CTCAATGCCT CTTCTGGAAT GAAACCTGAA AGAAGCCTTC AAAATAATGG TTTATTGACT
    ACTCTTAGTC AACACTACTT TTTATTTATT GGAACACTTT CTTGCCACCC TCATGGCGTC
    AAAATGCTGG AGAAATGCAG CGTATTTCAA TGTTTACTTA ACCTTTGCTC CTTGAAAAAC
    CAAGATCACT TGCTAAAACT GACCGTTTCA AGCTTGGACT ATAGTAGAGA TGGTTTGGCC
    AGGGTCATCC TTTCCAAAAT CTTAACAGCA GCCACTGATG CCTGCAGACT GTATGCAACA
    AAACATTTAA GAGTGTTATT GAGAGCTAAT GTTGAATTCT TCAATAATTG GGGAATTGAA
    TTACTAGTGA CCCAGCTGCA TGATAAAAAC AAAACGATTT CTTCTGAGGC CCTTGATATT
    CTCGATGAAG CATGTGAAGA CAAGGCCAAT CTTCATGCAC TTATTCAGAT GAAGCCAGCT
    TTATCTCACC TTGGGGACAA GGGTTTGCTT CTCCTCCTGA GATTTCTCTC CATTCCAAAA
    GGGTTTTCCT ATTTGAATGA AAGGGGTTAT GTAACAAAAC AGTTGGAAAA GTGGCACAAG
    GAATATAATT CAAAATACGT TGACTTGATT GAAGAACAGC TTAATGAAGC ACTTACCACT
    TACCGGAAGC CAATTGATGG TGACAACTAT GTTCGTCGGA GTAACCAAAG ATTACAGCGT
    CCTCATGTCT ACCTACCTGT ACATCTTTAT GGACAACTGG TACACCACAA AACAGGCTGT
    CATTTATTGG AAGTACAGAA TATTATTACA GAACTCTGTC ACAATGTTCG TACACCAGAT
    TTGGATAAAT GGGAAGAAAT TAAAAAACTA AAAGCATCTC TTTGGGCCTT GGGAAATATT
    GGCTCATCTA ATTGGGGTCT CAATTTGCTA CAAGAAGAAA ACGTGATTCC AGATATCCTG
    AAACTTGCAA AACAGTGTGA AGTTCTTTCA GTCAGAGGGA CCTGTGTATA TGTTCTTGGG
    CTCATAGCCA AAACCAAGCA AGGCTGTGAT ATTCTAAAAT GTCACAATTG GGATGCTGTA
    AGGCACAGTC GTAAACATTT GTGGCCAGTG GTTCCAGACG ATGTAGAACA ACTCTGTAAT
    GAACTCTCAT CTGTCCCGAG CACCCTAAGT TTGAACTCGG AGTCAACAAG CTCTAGGCAT
    AATAGTGAGA GTGAATCTGC ACCGTCAAGT ATGTTCATCA TGGAGGATGA CCGGTTTGGC
    AGCAGCTCTA CCAGTACATT TTTCCTTGAC ATCAATGAAG ATGCAGAGCC AGCATTTTAT
    GATCGACCTG GACCTATAAA GGATAAAAAT CCATTCCCTT TCTTTGCTTC TAGTAAACTT
    GTGAAGAATC GTATCTTAAA TTCTCTTACT TTGCCTAATA AAAAACATCG TAGCAGCAGT
    GATCCAAAAG GAGGGAAACT GTCATCTGAA AATAAGACAA GCAACAGGCG GATCAGAACG
    CTTACAGAGC CCAGTGTTGA CTTTAATCAT AGTGATGATT TCACACCCAT ATCCACAGTA
    CAGAAAACAT TACAATTAGA AACTTCATTT GTTGGGAATA AGCACATTGA AGACACTGGT
    AGTACTCCAA GTATTGGAGA GAATGACTTA AAATTCACCA AGAGTCTTGG TACAGAGAAT
    CACAAAGAAA ACACAAGCCG AGAGAGATTA GTTGTAGAAA GTTCATCGAG CTCACATATG
    AAGATACGTA GCCAGAGTTT TAATACAGAT ACTACAACAA GTGGCATAAG TTCAATGAGT
    TCAAGTCCTT CACGAGAGAC AGTAGGTGTA GATGCTACGA CTGTGGACAC AGACTGTGGA
    AGTATGAGTA CTGTGGTAAG TACTAAAACT GTTAAGACAA GCCACTATTT GACGCCACAG
    TCTAACCATC TCTCTCTCTC TAAATCAAGC TCAGTGTCCC TGGTGCCTCC AGGTTCTTCT
    CACACACTTC CTAGAAGAGC ACAGTCCCTT AAAGCACCCT CTATTGCTAC AATTAAAAGT
    CTAGCAGACT ATAACTTTAG TTACACAAGT TCTAGAGATG CCTTTGGCTA CGCTACCCTG
    AAAAGATTAC AACAGCAAAG AATGCATCCA TCCTTATCTC ATTCTGAAGC TTTGGCATCC
    CCAGCAAAAG ATGTGCTGTT TACTGATACC ATCACCATGA AGGCCAACAG CTTTGAGTCC
    AGGTTAACAC CAAGCAGGAT CAATTTTAAA AAGAAGCATG TCGGGGGAAT CAGGAGCTTA
    AGACCTACAA TAACAAACAA CTTTTTCAGG TTCATGAAAG CTTTAAGTTA TGCTTCGCTA
    GATAAAGAAG ATTTATTAAG TCCTATTAAC CAAAATACGC TGCAGCGCTC CTCCTCCGTG
    AGGTCCATGG TGTCCAGTGC GACATACGGT AGCTCAGATG ACTATATTGG TCTTGCTCTT
    CCAGTAGATA TCAATGATAT ATTCCAGGTA AAGGATATTC CTTATTTTCA AACAAAAAAC
    ATACCTCCAC ATGATGATCG AGGTGCAAGA GTATTTGCCC ATGATGTTGG AGGTCTTCCA
    TCTGGAACTG GAGGTCTTGT AAAAAACTCT TTTCACTTGC TACGACAGCA GATGAGTCTT
    ACGGAAATAA TGAATTCAAT CCATTCAGAT GCTTCTCTGT TTTTAGAAAG TACAGAAGAC
    ACTGGACTAC AGGAACATAC AGATGATAAC TGCCTTTATT GTGTCTGTAT TGAAATTCTG
    GGTTTCCAGC CCAGTAACCA ACTGAGTGCA ATATGTAGTC ATTCAGACCT TCAAGACATT
    CCATATTCTG ATTGGTGTGA GCAGACTATC CATAATCCCT TAGAAGTCGT TCCCTCTAAA
    TTTTCTGGCA TTTCTGGATG CAGTGATGGA GTGTCTCAAG AAGGCTCAGC CAGCAGCACC
    AAAAGTACAG AATTGCTACT AGGTGTTAAA ACAATTCCAG ATGATACACC AATGTGCCGT
    ATACTCCTTC GTAAAGAAGT TCTGAGATTA GTCATTAATT TGAGTAGTTC AGTTTCAACT
    AAATGTCATG AAACTGGGCT TTTAACAATT AAAGAGAAGT ATCCTCAAAC ATTTGATGAC
    ATATGCCTTT ACTCTGAGGT TTCCCATTTG CTGTCACACT GCACATTTAG ACTTCCATGT
    CGGAGGTTCA TACAAGAATT ATTTCAGGAT GTACAGTTTT TACAAATGCA TGAAGAAGCA
    GAGGCTGTCT TGGCAACACC ACCAAAGCAA ACTATAGTCG ATACATCTGC TGAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314578.1
    CDS170..5209
    Translation

    Target ORF information:

    RefSeq Version XM_015459092.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459092.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGAAAGC TAGGCCATCT GAATAACTTT ACTAAGCTGC TTTGTGATAT TGGCCACAGT 
    GAAGAAAAAC TGGGTTTTAA CTATGAGGAT ATCATAATTT GCTTGCGGTT AGCTTTATTG
    AATGAAGCAA AAGAAGTGCG GGCAGCAGGG CTTCGAGCTC TTCGGTATCT CATCCAAGAC
    TCCAGTATTC TGCAGAAGGT GCTAAAATTG AAAGTGGACT ATTTAATAGC TAGGTGCATT
    GACATACAGC AGAGCAACGA GGTAGAGAGG ACACAAGCAC TTCGATTAGT CAGAAAGATG
    ATTACTGTGA ATGCTTCCTT GTTTCCTAGC TCTGTGGCCA ACTCATTAAT AGCAGTTGGA
    AATGATGGAC TTCAAGAAAG AGACAGAATG GTTCGAGCAT GCATTGCCAT TATCTGTGAA
    CTAGCACTTC AGAATCCAGA GGTGGTTGCC CTTCGAGGTG GACTGAACAC CATCTTGAAA
    AATGTGATTG ATTGCCAATT AAGTCGAATA AATGAGGCTC TAATTACTAC AATTTTGCAC
    CTTCTTAATC ATCCAAAGAC CCGACAGTAT GTACGAGCTG ATGTCGAATT AGAGCGAATT
    TTAGCACCCT ATACCGATTT TCACTACAGA CATAGTCCAG ATACAGCTGA AGGACAGCTA
    AAAGAAGACA GAGAAGCAAG GTTTCTAGCC AGTAAAATGG GAATCATAGC AACATTCCGG
    TCATGGGCAG GTATTATTAA TTTATGTAAA CCTGGAAACT CTGGGATTCA GTCTCTAATT
    GGAGTACTTT GCATTCCAAA TATGGAAATA AGGCGAGGTC TCCTTGAAGT GCTTTATGAT
    ATATTTCGTC TTCCTCTACC TGTTGTGACT GAAGAGTTCA TAGAAGCACT ACTCAGTGTA
    GATCCAGGTA GATTCCAAGA CAGTTGGAGG CTTTCAGATG GCTTTGTAGC AGCTGAGGCA
    AAAACTATTC TTCCTCATCG TGCCAGATCC AGGCCAGACC TCATGGATAA TTATTTGGCA
    CTGATACTCT CAGCATTTAT TCGTAATGGA CTTTTAGAAG GTTTGGTTGA GGTAATAACA
    AACAGTGACG ACCATATCTC AGTTAGAGCC ACCATCCTTT TAGGAGAACT TTTACATATG
    GCAAACACAA TTCTTCCTCA TTCACATAGC CATCATTTGC ACTGCCTGCC AACCCTAATG
    AATATGGCTG CATCCTTTGA TATCCCTAAG GAAAAGAGAT TGCGAGCCAG TGCAGCTTTG
    AACTGTTTAA AACGCTTCCA TGAAATGAAA AAACGAGGAC CTAAACCTTA TAGCCTTCAT
    TTAGATCACA TTATTCAGAA AGCAATTGCA ACACATCAGA AACGGGATCA GTATCTCCGA
    GCTCAGAAAG ATATATTTGT TCTTAAGGAT ACAGAGGAAG CTCTTCTAAT GAACCTTAGA
    GATAGCCAAG TCCTTCAACA TAAAGAGAAT CTTGAATGGA ATTGGAATCT TATTGGGACC
    ATTCTTAAGT GGCCAAATGT AAATCTAAGA AACTATAAAG ATGAGCAGTT GCACAGGTTT
    GTACGAAGAC TACTTTATTT TTACAAGCCC AGTAGTAAGT TATACGCCAA TCTGGATCTG
    GATTTTGCCA AGTCCAAGCA GCTCACAGTT GTGGGTTGTC AGTTTACAGA ATTTCTTCTT
    GAGTCTGAAG AGGATGGGCA GGGATACTTA GAAGATCTAG TAAAGGATAT TGTTCAGTGG
    CTCAATGCCT CTTCTGGAAT GAAACCTGAA AGAAGCCTTC AAAATAATGG TTTATTGACT
    ACTCTTAGTC AACACTACTT TTTATTTATT GGAACACTTT CTTGCCACCC TCATGGCGTC
    AAAATGCTGG AGAAATGCAG CGTATTTCAA TGTTTACTTA ACCTTTGCTC CTTGAAAAAC
    CAAGATCACT TGCTAAAACT GACCGTTTCA AGCTTGGACT ATAGTAGAGA TGGTTTGGCC
    AGGGTCATCC TTTCCAAAAT CTTAACAGCA GCCACTGATG CCTGCAGACT GTATGCAACA
    AAACATTTAA GAGTGTTATT GAGAGCTAAT GTTGAATTCT TCAATAATTG GGGAATTGAA
    TTACTAGTGA CCCAGCTGCA TGATAAAAAC AAAACGATTT CTTCTGAGGC CCTTGATATT
    CTCGATGAAG CATGTGAAGA CAAGGCCAAT CTTCATGCAC TTATTCAGAT GAAGCCAGCT
    TTATCTCACC TTGGGGACAA GGGTTTGCTT CTCCTCCTGA GATTTCTCTC CATTCCAAAA
    GGGTTTTCCT ATTTGAATGA AAGGGGTTAT GTAACAAAAC AGTTGGAAAA GTGGCACAAG
    GAATATAATT CAAAATACGT TGACTTGATT GAAGAACAGC TTAATGAAGC ACTTACCACT
    TACCGGAAGC CAATTGATGG TGACAACTAT GTTCGTCGGA GTAACCAAAG ATTACAGCGT
    CCTCATGTCT ACCTACCTGT ACATCTTTAT GGACAACTGG TACACCACAA AACAGGCTGT
    CATTTATTGG AAGTACAGAA TATTATTACA GAACTCTGTC ACAATGTTCG TACACCAGAT
    TTGGATAAAT GGGAAGAAAT TAAAAAACTA AAAGCATCTC TTTGGGCCTT GGGAAATATT
    GGCTCATCTA ATTGGGGTCT CAATTTGCTA CAAGAAGAAA ACGTGATTCC AGATATCCTG
    AAACTTGCAA AACAGTGTGA AGTTCTTTCA GTCAGAGGGA CCTGTGTATA TGTTCTTGGG
    CTCATAGCCA AAACCAAGCA AGGCTGTGAT ATTCTAAAAT GTCACAATTG GGATGCTGTA
    AGGCACAGTC GTAAACATTT GTGGCCAGTG GTTCCAGACG ATGTAGAACA ACTCTGTAAT
    GAACTCTCAT CTGTCCCGAG CACCCTAAGT TTGAACTCGG AGTCAACAAG CTCTAGGCAT
    AATAGTGAGA GTGAATCTGC ACCGTCAAGT ATGTTCATCA TGGAGGATGA CCGGTTTGGC
    AGCAGCTCTA CCAGTACATT TTTCCTTGAC ATCAATGAAG ATGCAGAGCC AGCATTTTAT
    GATCGACCTG GACCTATAAA GGATAAAAAT CCATTCCCTT TCTTTGCTTC TAGTAAACTT
    GTGAAGAATC GTATCTTAAA TTCTCTTACT TTGCCTAATA AAAAACATCG TAGCAGCAGT
    GATCCAAAAG GAGGGAAACT GTCATCTGAA AATAAGACAA GCAACAGGCG GATCAGAACG
    CTTACAGAGC CCAGTGTTGA CTTTAATCAT AGTGATGATT TCACACCCAT ATCCACAGTA
    CAGAAAACAT TACAATTAGA AACTTCATTT GTTGGGAATA AGCACATTGA AGACACTGGT
    AGTACTCCAA GTATTGGAGA GAATGACTTA AAATTCACCA AGAGTCTTGG TACAGAGAAT
    CACAAAGAAA ACACAAGCCG AGAGAGATTA GTTGTAGAAA GTTCATCGAG CTCACATATG
    AAGATACGTA GCCAGAGTTT TAATACAGAT ACTACAACAA GTGGCATAAG TTCAATGAGT
    TCAAGTCCTT CACGAGAGAC AGTAGGTGTA GATGCTACGA CTGTGGACAC AGACTGTGGA
    AGTATGAGTA CTGTGGTAAG TACTAAAACT GTTAAGACAA GCCACTATTT GACGCCACAG
    TCTAACCATC TCTCTCTCTC TAAATCAAGC TCAGTGTCCC TGGTGCCTCC AGGTTCTTCT
    CACACACTTC CTAGAAGAGC ACAGTCCCTT AAAGCACCCT CTATTGCTAC AATTAAAAGT
    CTAGCAGACT ATAACTTTAG TTACACAAGT TCTAGAGATG CCTTTGGCTA CGCTACCCTG
    AAAAGATTAC AACAGCAAAG AATGCATCCA TCCTTATCTC ATTCTGAAGC TTTGGCATCC
    CCAGCAAAAG ATGTGCTGTT TACTGATACC ATCACCATGA AGGCCAACAG CTTTGAGTCC
    AGGTTAACAC CAAGCAGGAT CAATTTTAAA AAGAAGCATG TCGGGGGAAT CAGGAGCTTA
    AGACCTACAA TAACAAACAA CTTTTTCAGG TTCATGAAAG CTTTAAGTTA TGCTTCGCTA
    GATAAAGAAG ATTTATTAAG TCCTATTAAC CAAAATACGC TGCAGCGCTC CTCCTCCGTG
    AGGTCCATGG TGTCCAGTGC GACATACGGT AGCTCAGATG ACTATATTGG TCTTGCTCTT
    CCAGTAGATA TCAATGATAT ATTCCAGGTA AAGGATATTC CTTATTTTCA AACAAAAAAC
    ATACCTCCAC ATGATGATCG AGGTGCAAGA GTATTTGCCC ATGATGTTGG AGGTCTTCCA
    TCTGGAACTG GAGGTCTTGT AAAAAACTCT TTTCACTTGC TACGACAGCA GATGAGTCTT
    ACGGAAATAA TGAATTCAAT CCATTCAGAT GCTTCTCTGT TTTTAGAAAG TACAGAAGAC
    ACTGGACTAC AGGAACATAC AGATGATAAC TGCCTTTATT GTGTCTGTAT TGAAATTCTG
    GGTTTCCAGC CCAGTAACCA ACTGAGTGCA ATATGTAGTC ATTCAGACCT TCAAGACATT
    CCATATTCTG ATTGGTGTGA GCAGACTATC CATAATCCCT TAGAAGTCGT TCCCTCTAAA
    TTTTCTGGCA TTTCTGGATG CAGTGATGGA GTGTCTCAAG AAGGCTCAGC CAGCAGCACC
    AAAAGTACAG AATTGCTACT AGGTGTTAAA ACAATTCCAG ATGATACACC AATGTGCCGT
    ATACTCCTTC GTAAAGAAGT TCTGAGATTA GTCATTAATT TGAGTAGTTC AGTTTCAACT
    AAATGTCATG AAACTGGGCT TTTAACAATT AAAGAGAAGT ATCCTCAAAC ATTTGATGAC
    ATATGCCTTT ACTCTGAGGT TTCCCATTTG CTGTCACACT GCACATTTAG ACTTCCATGT
    CGGAGGTTCA TACAAGAATT ATTTCAGGAT GTACAGTTTT TACAAATGCA TGAAGAAGCA
    GAGGCTGTCT TGGCAACACC ACCAAAGCAA ACTATAGTCG ATACATCTGC TGAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30669
    Clone ID Related Accession (Same CDS sequence) XM_015459092.1 , XM_024981298.1
    Accession Version XM_024981298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGAAAGC TAGGCCATCT GAATAACTTT ACTAAGCTGC TTTGTGATAT TGGCCACAGT 
    GAAGAAAAAC TGGGTTTTAA CTATGAGGAT ATCATAATTT GCTTGCGGTT AGCTTTATTG
    AATGAAGCAA AAGAAGTGCG GGCAGCAGGG CTTCGAGCTC TTCGGTATCT CATCCAAGAC
    TCCAGTATTC TGCAGAAGGT GCTAAAATTG AAAGTGGACT ATTTAATAGC TAGGTGCATT
    GACATACAGC AGAGCAACGA GGTAGAGAGG ACACAAGCAC TTCGATTAGT CAGAAAGATG
    ATTACTGTGA ATGCTTCCTT GTTTCCTAGC TCTGTGGCCA ACTCATTAAT AGCAGTTGGA
    AATGATGGAC TTCAAGAAAG AGACAGAATG GTTCGAGCAT GCATTGCCAT TATCTGTGAA
    CTAGCACTTC AGAATCCAGA GGTGGTTGCC CTTCGAGGTG GACTGAACAC CATCTTGAAA
    AATGTGATTG ATTGCCAATT AAGTCGAATA AATGAGGCTC TAATTACTAC AATTTTGCAC
    CTTCTTAATC ATCCAAAGAC CCGACAGTAT GTACGAGCTG ATGTCGAATT AGAGCGAATT
    TTAGCACCCT ATACCGATTT TCACTACAGA CATAGTCCAG ATACAGCTGA AGGACAGCTA
    AAAGAAGACA GAGAAGCAAG GTTTCTAGCC AGTAAAATGG GAATCATAGC AACATTCCGG
    TCATGGGCAG GTATTATTAA TTTATGTAAA CCTGGAAACT CTGGGATTCA GTCTCTAATT
    GGAGTACTTT GCATTCCAAA TATGGAAATA AGGCGAGGTC TCCTTGAAGT GCTTTATGAT
    ATATTTCGTC TTCCTCTACC TGTTGTGACT GAAGAGTTCA TAGAAGCACT ACTCAGTGTA
    GATCCAGGTA GATTCCAAGA CAGTTGGAGG CTTTCAGATG GCTTTGTAGC AGCTGAGGCA
    AAAACTATTC TTCCTCATCG TGCCAGATCC AGGCCAGACC TCATGGATAA TTATTTGGCA
    CTGATACTCT CAGCATTTAT TCGTAATGGA CTTTTAGAAG GTTTGGTTGA GGTAATAACA
    AACAGTGACG ACCATATCTC AGTTAGAGCC ACCATCCTTT TAGGAGAACT TTTACATATG
    GCAAACACAA TTCTTCCTCA TTCACATAGC CATCATTTGC ACTGCCTGCC AACCCTAATG
    AATATGGCTG CATCCTTTGA TATCCCTAAG GAAAAGAGAT TGCGAGCCAG TGCAGCTTTG
    AACTGTTTAA AACGCTTCCA TGAAATGAAA AAACGAGGAC CTAAACCTTA TAGCCTTCAT
    TTAGATCACA TTATTCAGAA AGCAATTGCA ACACATCAGA AACGGGATCA GTATCTCCGA
    GCTCAGAAAG ATATATTTGT TCTTAAGGAT ACAGAGGAAG CTCTTCTAAT GAACCTTAGA
    GATAGCCAAG TCCTTCAACA TAAAGAGAAT CTTGAATGGA ATTGGAATCT TATTGGGACC
    ATTCTTAAGT GGCCAAATGT AAATCTAAGA AACTATAAAG ATGAGCAGTT GCACAGGTTT
    GTACGAAGAC TACTTTATTT TTACAAGCCC AGTAGTAAGT TATACGCCAA TCTGGATCTG
    GATTTTGCCA AGTCCAAGCA GCTCACAGTT GTGGGTTGTC AGTTTACAGA ATTTCTTCTT
    GAGTCTGAAG AGGATGGGCA GGGATACTTA GAAGATCTAG TAAAGGATAT TGTTCAGTGG
    CTCAATGCCT CTTCTGGAAT GAAACCTGAA AGAAGCCTTC AAAATAATGG TTTATTGACT
    ACTCTTAGTC AACACTACTT TTTATTTATT GGAACACTTT CTTGCCACCC TCATGGCGTC
    AAAATGCTGG AGAAATGCAG CGTATTTCAA TGTTTACTTA ACCTTTGCTC CTTGAAAAAC
    CAAGATCACT TGCTAAAACT GACCGTTTCA AGCTTGGACT ATAGTAGAGA TGGTTTGGCC
    AGGGTCATCC TTTCCAAAAT CTTAACAGCA GCCACTGATG CCTGCAGACT GTATGCAACA
    AAACATTTAA GAGTGTTATT GAGAGCTAAT GTTGAATTCT TCAATAATTG GGGAATTGAA
    TTACTAGTGA CCCAGCTGCA TGATAAAAAC AAAACGATTT CTTCTGAGGC CCTTGATATT
    CTCGATGAAG CATGTGAAGA CAAGGCCAAT CTTCATGCAC TTATTCAGAT GAAGCCAGCT
    TTATCTCACC TTGGGGACAA GGGTTTGCTT CTCCTCCTGA GATTTCTCTC CATTCCAAAA
    GGGTTTTCCT ATTTGAATGA AAGGGGTTAT GTAACAAAAC AGTTGGAAAA GTGGCACAAG
    GAATATAATT CAAAATACGT TGACTTGATT GAAGAACAGC TTAATGAAGC ACTTACCACT
    TACCGGAAGC CAATTGATGG TGACAACTAT GTTCGTCGGA GTAACCAAAG ATTACAGCGT
    CCTCATGTCT ACCTACCTGT ACATCTTTAT GGACAACTGG TACACCACAA AACAGGCTGT
    CATTTATTGG AAGTACAGAA TATTATTACA GAACTCTGTC ACAATGTTCG TACACCAGAT
    TTGGATAAAT GGGAAGAAAT TAAAAAACTA AAAGCATCTC TTTGGGCCTT GGGAAATATT
    GGCTCATCTA ATTGGGGTCT CAATTTGCTA CAAGAAGAAA ACGTGATTCC AGATATCCTG
    AAACTTGCAA AACAGTGTGA AGTTCTTTCA GTCAGAGGGA CCTGTGTATA TGTTCTTGGG
    CTCATAGCCA AAACCAAGCA AGGCTGTGAT ATTCTAAAAT GTCACAATTG GGATGCTGTA
    AGGCACAGTC GTAAACATTT GTGGCCAGTG GTTCCAGACG ATGTAGAACA ACTCTGTAAT
    GAACTCTCAT CTGTCCCGAG CACCCTAAGT TTGAACTCGG AGTCAACAAG CTCTAGGCAT
    AATAGTGAGA GTGAATCTGC ACCGTCAAGT ATGTTCATCA TGGAGGATGA CCGGTTTGGC
    AGCAGCTCTA CCAGTACATT TTTCCTTGAC ATCAATGAAG ATGCAGAGCC AGCATTTTAT
    GATCGACCTG GACCTATAAA GGATAAAAAT CCATTCCCTT TCTTTGCTTC TAGTAAACTT
    GTGAAGAATC GTATCTTAAA TTCTCTTACT TTGCCTAATA AAAAACATCG TAGCAGCAGT
    GATCCAAAAG GAGGGAAACT GTCATCTGAA AATAAGACAA GCAACAGGCG GATCAGAACG
    CTTACAGAGC CCAGTGTTGA CTTTAATCAT AGTGATGATT TCACACCCAT ATCCACAGTA
    CAGAAAACAT TACAATTAGA AACTTCATTT GTTGGGAATA AGCACATTGA AGACACTGGT
    AGTACTCCAA GTATTGGAGA GAATGACTTA AAATTCACCA AGAGTCTTGG TACAGAGAAT
    CACAAAGAAA ACACAAGCCG AGAGAGATTA GTTGTAGAAA GTTCATCGAG CTCACATATG
    AAGATACGTA GCCAGAGTTT TAATACAGAT ACTACAACAA GTGGCATAAG TTCAATGAGT
    TCAAGTCCTT CACGAGAGAC AGTAGGTGTA GATGCTACGA CTGTGGACAC AGACTGTGGA
    AGTATGAGTA CTGTGGTAAG TACTAAAACT GTTAAGACAA GCCACTATTT GACGCCACAG
    TCTAACCATC TCTCTCTCTC TAAATCAAGC TCAGTGTCCC TGGTGCCTCC AGGTTCTTCT
    CACACACTTC CTAGAAGAGC ACAGTCCCTT AAAGCACCCT CTATTGCTAC AATTAAAAGT
    CTAGCAGACT ATAACTTTAG TTACACAAGT TCTAGAGATG CCTTTGGCTA CGCTACCCTG
    AAAAGATTAC AACAGCAAAG AATGCATCCA TCCTTATCTC ATTCTGAAGC TTTGGCATCC
    CCAGCAAAAG ATGTGCTGTT TACTGATACC ATCACCATGA AGGCCAACAG CTTTGAGTCC
    AGGTTAACAC CAAGCAGGAT CAATTTTAAA AAGAAGCATG TCGGGGGAAT CAGGAGCTTA
    AGACCTACAA TAACAAACAA CTTTTTCAGG TTCATGAAAG CTTTAAGTTA TGCTTCGCTA
    GATAAAGAAG ATTTATTAAG TCCTATTAAC CAAAATACGC TGCAGCGCTC CTCCTCCGTG
    AGGTCCATGG TGTCCAGTGC GACATACGGT AGCTCAGATG ACTATATTGG TCTTGCTCTT
    CCAGTAGATA TCAATGATAT ATTCCAGGTA AAGGATATTC CTTATTTTCA AACAAAAAAC
    ATACCTCCAC ATGATGATCG AGGTGCAAGA GTATTTGCCC ATGATGTTGG AGGTCTTCCA
    TCTGGAACTG GAGGTCTTGT AAAAAACTCT TTTCACTTGC TACGACAGCA GATGAGTCTT
    ACGGAAATAA TGAATTCAAT CCATTCAGAT GCTTCTCTGT TTTTAGAAAG TACAGAAGAC
    ACTGGACTAC AGGAACATAC AGATGATAAC TGCCTTTATT GTGTCTGTAT TGAAATTCTG
    GGTTTCCAGC CCAGTAACCA ACTGAGTGCA ATATGTAGTC ATTCAGACCT TCAAGACATT
    CCATATTCTG ATTGGTGTGA GCAGACTATC CATAATCCCT TAGAAGTCGT TCCCTCTAAA
    TTTTCTGGCA TTTCTGGATG CAGTGATGGA GTGTCTCAAG AAGGCTCAGC CAGCAGCACC
    AAAAGTACAG AATTGCTACT AGGTGTTAAA ACAATTCCAG ATGATACACC AATGTGCCGT
    ATACTCCTTC GTAAAGAAGT TCTGAGATTA GTCATTAATT TGAGTAGTTC AGTTTCAACT
    AAATGTCATG AAACTGGGCT TTTAACAATT AAAGAGAAGT ATCCTCAAAC ATTTGATGAC
    ATATGCCTTT ACTCTGAGGT TTCCCATTTG CTGTCACACT GCACATTTAG ACTTCCATGT
    CGGAGGTTCA TACAAGAATT ATTTCAGGAT GTACAGTTTT TACAAATGCA TGAAGAAGCA
    GAGGCTGTCT TGGCAACACC ACCAAAGCAA ACTATAGTCG ATACATCTGC TGAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837066.1
    CDS299..5338
    Translation

    Target ORF information:

    RefSeq Version XM_024981298.1
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAGAAAGC TAGGCCATCT GAATAACTTT ACTAAGCTGC TTTGTGATAT TGGCCACAGT 
    GAAGAAAAAC TGGGTTTTAA CTATGAGGAT ATCATAATTT GCTTGCGGTT AGCTTTATTG
    AATGAAGCAA AAGAAGTGCG GGCAGCAGGG CTTCGAGCTC TTCGGTATCT CATCCAAGAC
    TCCAGTATTC TGCAGAAGGT GCTAAAATTG AAAGTGGACT ATTTAATAGC TAGGTGCATT
    GACATACAGC AGAGCAACGA GGTAGAGAGG ACACAAGCAC TTCGATTAGT CAGAAAGATG
    ATTACTGTGA ATGCTTCCTT GTTTCCTAGC TCTGTGGCCA ACTCATTAAT AGCAGTTGGA
    AATGATGGAC TTCAAGAAAG AGACAGAATG GTTCGAGCAT GCATTGCCAT TATCTGTGAA
    CTAGCACTTC AGAATCCAGA GGTGGTTGCC CTTCGAGGTG GACTGAACAC CATCTTGAAA
    AATGTGATTG ATTGCCAATT AAGTCGAATA AATGAGGCTC TAATTACTAC AATTTTGCAC
    CTTCTTAATC ATCCAAAGAC CCGACAGTAT GTACGAGCTG ATGTCGAATT AGAGCGAATT
    TTAGCACCCT ATACCGATTT TCACTACAGA CATAGTCCAG ATACAGCTGA AGGACAGCTA
    AAAGAAGACA GAGAAGCAAG GTTTCTAGCC AGTAAAATGG GAATCATAGC AACATTCCGG
    TCATGGGCAG GTATTATTAA TTTATGTAAA CCTGGAAACT CTGGGATTCA GTCTCTAATT
    GGAGTACTTT GCATTCCAAA TATGGAAATA AGGCGAGGTC TCCTTGAAGT GCTTTATGAT
    ATATTTCGTC TTCCTCTACC TGTTGTGACT GAAGAGTTCA TAGAAGCACT ACTCAGTGTA
    GATCCAGGTA GATTCCAAGA CAGTTGGAGG CTTTCAGATG GCTTTGTAGC AGCTGAGGCA
    AAAACTATTC TTCCTCATCG TGCCAGATCC AGGCCAGACC TCATGGATAA TTATTTGGCA
    CTGATACTCT CAGCATTTAT TCGTAATGGA CTTTTAGAAG GTTTGGTTGA GGTAATAACA
    AACAGTGACG ACCATATCTC AGTTAGAGCC ACCATCCTTT TAGGAGAACT TTTACATATG
    GCAAACACAA TTCTTCCTCA TTCACATAGC CATCATTTGC ACTGCCTGCC AACCCTAATG
    AATATGGCTG CATCCTTTGA TATCCCTAAG GAAAAGAGAT TGCGAGCCAG TGCAGCTTTG
    AACTGTTTAA AACGCTTCCA TGAAATGAAA AAACGAGGAC CTAAACCTTA TAGCCTTCAT
    TTAGATCACA TTATTCAGAA AGCAATTGCA ACACATCAGA AACGGGATCA GTATCTCCGA
    GCTCAGAAAG ATATATTTGT TCTTAAGGAT ACAGAGGAAG CTCTTCTAAT GAACCTTAGA
    GATAGCCAAG TCCTTCAACA TAAAGAGAAT CTTGAATGGA ATTGGAATCT TATTGGGACC
    ATTCTTAAGT GGCCAAATGT AAATCTAAGA AACTATAAAG ATGAGCAGTT GCACAGGTTT
    GTACGAAGAC TACTTTATTT TTACAAGCCC AGTAGTAAGT TATACGCCAA TCTGGATCTG
    GATTTTGCCA AGTCCAAGCA GCTCACAGTT GTGGGTTGTC AGTTTACAGA ATTTCTTCTT
    GAGTCTGAAG AGGATGGGCA GGGATACTTA GAAGATCTAG TAAAGGATAT TGTTCAGTGG
    CTCAATGCCT CTTCTGGAAT GAAACCTGAA AGAAGCCTTC AAAATAATGG TTTATTGACT
    ACTCTTAGTC AACACTACTT TTTATTTATT GGAACACTTT CTTGCCACCC TCATGGCGTC
    AAAATGCTGG AGAAATGCAG CGTATTTCAA TGTTTACTTA ACCTTTGCTC CTTGAAAAAC
    CAAGATCACT TGCTAAAACT GACCGTTTCA AGCTTGGACT ATAGTAGAGA TGGTTTGGCC
    AGGGTCATCC TTTCCAAAAT CTTAACAGCA GCCACTGATG CCTGCAGACT GTATGCAACA
    AAACATTTAA GAGTGTTATT GAGAGCTAAT GTTGAATTCT TCAATAATTG GGGAATTGAA
    TTACTAGTGA CCCAGCTGCA TGATAAAAAC AAAACGATTT CTTCTGAGGC CCTTGATATT
    CTCGATGAAG CATGTGAAGA CAAGGCCAAT CTTCATGCAC TTATTCAGAT GAAGCCAGCT
    TTATCTCACC TTGGGGACAA GGGTTTGCTT CTCCTCCTGA GATTTCTCTC CATTCCAAAA
    GGGTTTTCCT ATTTGAATGA AAGGGGTTAT GTAACAAAAC AGTTGGAAAA GTGGCACAAG
    GAATATAATT CAAAATACGT TGACTTGATT GAAGAACAGC TTAATGAAGC ACTTACCACT
    TACCGGAAGC CAATTGATGG TGACAACTAT GTTCGTCGGA GTAACCAAAG ATTACAGCGT
    CCTCATGTCT ACCTACCTGT ACATCTTTAT GGACAACTGG TACACCACAA AACAGGCTGT
    CATTTATTGG AAGTACAGAA TATTATTACA GAACTCTGTC ACAATGTTCG TACACCAGAT
    TTGGATAAAT GGGAAGAAAT TAAAAAACTA AAAGCATCTC TTTGGGCCTT GGGAAATATT
    GGCTCATCTA ATTGGGGTCT CAATTTGCTA CAAGAAGAAA ACGTGATTCC AGATATCCTG
    AAACTTGCAA AACAGTGTGA AGTTCTTTCA GTCAGAGGGA CCTGTGTATA TGTTCTTGGG
    CTCATAGCCA AAACCAAGCA AGGCTGTGAT ATTCTAAAAT GTCACAATTG GGATGCTGTA
    AGGCACAGTC GTAAACATTT GTGGCCAGTG GTTCCAGACG ATGTAGAACA ACTCTGTAAT
    GAACTCTCAT CTGTCCCGAG CACCCTAAGT TTGAACTCGG AGTCAACAAG CTCTAGGCAT
    AATAGTGAGA GTGAATCTGC ACCGTCAAGT ATGTTCATCA TGGAGGATGA CCGGTTTGGC
    AGCAGCTCTA CCAGTACATT TTTCCTTGAC ATCAATGAAG ATGCAGAGCC AGCATTTTAT
    GATCGACCTG GACCTATAAA GGATAAAAAT CCATTCCCTT TCTTTGCTTC TAGTAAACTT
    GTGAAGAATC GTATCTTAAA TTCTCTTACT TTGCCTAATA AAAAACATCG TAGCAGCAGT
    GATCCAAAAG GAGGGAAACT GTCATCTGAA AATAAGACAA GCAACAGGCG GATCAGAACG
    CTTACAGAGC CCAGTGTTGA CTTTAATCAT AGTGATGATT TCACACCCAT ATCCACAGTA
    CAGAAAACAT TACAATTAGA AACTTCATTT GTTGGGAATA AGCACATTGA AGACACTGGT
    AGTACTCCAA GTATTGGAGA GAATGACTTA AAATTCACCA AGAGTCTTGG TACAGAGAAT
    CACAAAGAAA ACACAAGCCG AGAGAGATTA GTTGTAGAAA GTTCATCGAG CTCACATATG
    AAGATACGTA GCCAGAGTTT TAATACAGAT ACTACAACAA GTGGCATAAG TTCAATGAGT
    TCAAGTCCTT CACGAGAGAC AGTAGGTGTA GATGCTACGA CTGTGGACAC AGACTGTGGA
    AGTATGAGTA CTGTGGTAAG TACTAAAACT GTTAAGACAA GCCACTATTT GACGCCACAG
    TCTAACCATC TCTCTCTCTC TAAATCAAGC TCAGTGTCCC TGGTGCCTCC AGGTTCTTCT
    CACACACTTC CTAGAAGAGC ACAGTCCCTT AAAGCACCCT CTATTGCTAC AATTAAAAGT
    CTAGCAGACT ATAACTTTAG TTACACAAGT TCTAGAGATG CCTTTGGCTA CGCTACCCTG
    AAAAGATTAC AACAGCAAAG AATGCATCCA TCCTTATCTC ATTCTGAAGC TTTGGCATCC
    CCAGCAAAAG ATGTGCTGTT TACTGATACC ATCACCATGA AGGCCAACAG CTTTGAGTCC
    AGGTTAACAC CAAGCAGGAT CAATTTTAAA AAGAAGCATG TCGGGGGAAT CAGGAGCTTA
    AGACCTACAA TAACAAACAA CTTTTTCAGG TTCATGAAAG CTTTAAGTTA TGCTTCGCTA
    GATAAAGAAG ATTTATTAAG TCCTATTAAC CAAAATACGC TGCAGCGCTC CTCCTCCGTG
    AGGTCCATGG TGTCCAGTGC GACATACGGT AGCTCAGATG ACTATATTGG TCTTGCTCTT
    CCAGTAGATA TCAATGATAT ATTCCAGGTA AAGGATATTC CTTATTTTCA AACAAAAAAC
    ATACCTCCAC ATGATGATCG AGGTGCAAGA GTATTTGCCC ATGATGTTGG AGGTCTTCCA
    TCTGGAACTG GAGGTCTTGT AAAAAACTCT TTTCACTTGC TACGACAGCA GATGAGTCTT
    ACGGAAATAA TGAATTCAAT CCATTCAGAT GCTTCTCTGT TTTTAGAAAG TACAGAAGAC
    ACTGGACTAC AGGAACATAC AGATGATAAC TGCCTTTATT GTGTCTGTAT TGAAATTCTG
    GGTTTCCAGC CCAGTAACCA ACTGAGTGCA ATATGTAGTC ATTCAGACCT TCAAGACATT
    CCATATTCTG ATTGGTGTGA GCAGACTATC CATAATCCCT TAGAAGTCGT TCCCTCTAAA
    TTTTCTGGCA TTTCTGGATG CAGTGATGGA GTGTCTCAAG AAGGCTCAGC CAGCAGCACC
    AAAAGTACAG AATTGCTACT AGGTGTTAAA ACAATTCCAG ATGATACACC AATGTGCCGT
    ATACTCCTTC GTAAAGAAGT TCTGAGATTA GTCATTAATT TGAGTAGTTC AGTTTCAACT
    AAATGTCATG AAACTGGGCT TTTAACAATT AAAGAGAAGT ATCCTCAAAC ATTTGATGAC
    ATATGCCTTT ACTCTGAGGT TTCCCATTTG CTGTCACACT GCACATTTAG ACTTCCATGT
    CGGAGGTTCA TACAAGAATT ATTTCAGGAT GTACAGTTTT TACAAATGCA TGAAGAAGCA
    GAGGCTGTCT TGGCAACACC ACCAAAGCAA ACTATAGTCG ATACATCTGC TGAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51387
    Clone ID Related Accession (Same CDS sequence) XM_010816809.2 , XM_010816809.3
    Accession Version XM_010816809.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGATC
    AATTTTAAAA AGAAGCATGT CGGGGGAATC AGGAGCTTAA GACCTACAAT AACAAACAAC
    TTTTTCAGGT TCATGAAAGC TTTAAGTTAT GCTTCGCTAG ATAAAGAAGA TTTATTAAGT
    CCTATTAACC AAAATACGCT GCAGCGCTCC TCCTCCGTGA GGTCCATGGT GTCCAGTGCG
    ACATACGGTA GCTCAGATGA CTATATTGGT CTTGCTCTTC CAGTAGATAT CAATGATATA
    TTCCAGGTAA AGGATATTCC TTATTTTCAA ACAAAAAACA TACCTCCACA TGATGATCGA
    GGTGCAAGAG TATTTGCCCA TGATGTTGGA GGTCTTCCAT CTGGAACTGG AGGTCTTGTA
    AAAAACTCTT TTCACTTGCT ACGACAGCAG ATGAGTCTTA CGGAAATAAT GAATTCAATC
    CATTCAGATG CTTCTCTGTT TTTAGAAAGT ACAGAAGACA CTGGACTACA GGAACATACA
    GATGATAACT GCCTTTATTG TGTCTGTATT GAAATTCTGG GTTTCCAGCC CAGTAACCAA
    CTGAGTGCAA TATGTAGTCA TTCAGACCTT CAAGACATTC CATATTCTGA TTGGTGTGAG
    CAGACTATCC ATAATCCCTT AGAAGTCGTT CCCTCTAAAT TTTCTGGCAT TTCTGGATGC
    AGTGATGGAG TGTCTCAAGA AGGCTCAGCC AGCAGCACCA AAAGTACAGA ATTGCTACTA
    GGTGTTAAAA CAATTCCAGA TGATACACCA ATGTGCCGTA TACTCCTTCG TAAAGAAGTT
    CTGAGATTAG TCATTAATTT GAGTAGTTCA GTTTCAACTA AATGTCATGA AACTGGGCTT
    TTAACAATTA AAGAGAAGTA TCCTCAAACA TTTGATGACA TATGCCTTTA CTCTGAGGTT
    TCCCATTTGC TGTCACACTG CACATTTAGA CTTCCATGTC GGAGGTTCAT ACAAGAATTA
    TTTCAGGATG TACAGTTTTT ACAAATGCAT GAAGAAGCAG AGGCTGTCTT GGCAACACCA
    CCAAAGCAAA CTATAGTCGA TACATCTGCT GAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815111.1
    CDS59..5257
    Translation

    Target ORF information:

    RefSeq Version XM_010816809.2
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGATC
    AATTTTAAAA AGAAGCATGT CGGGGGAATC AGGAGCTTAA GACCTACAAT AACAAACAAC
    TTTTTCAGGT TCATGAAAGC TTTAAGTTAT GCTTCGCTAG ATAAAGAAGA TTTATTAAGT
    CCTATTAACC AAAATACGCT GCAGCGCTCC TCCTCCGTGA GGTCCATGGT GTCCAGTGCG
    ACATACGGTA GCTCAGATGA CTATATTGGT CTTGCTCTTC CAGTAGATAT CAATGATATA
    TTCCAGGTAA AGGATATTCC TTATTTTCAA ACAAAAAACA TACCTCCACA TGATGATCGA
    GGTGCAAGAG TATTTGCCCA TGATGTTGGA GGTCTTCCAT CTGGAACTGG AGGTCTTGTA
    AAAAACTCTT TTCACTTGCT ACGACAGCAG ATGAGTCTTA CGGAAATAAT GAATTCAATC
    CATTCAGATG CTTCTCTGTT TTTAGAAAGT ACAGAAGACA CTGGACTACA GGAACATACA
    GATGATAACT GCCTTTATTG TGTCTGTATT GAAATTCTGG GTTTCCAGCC CAGTAACCAA
    CTGAGTGCAA TATGTAGTCA TTCAGACCTT CAAGACATTC CATATTCTGA TTGGTGTGAG
    CAGACTATCC ATAATCCCTT AGAAGTCGTT CCCTCTAAAT TTTCTGGCAT TTCTGGATGC
    AGTGATGGAG TGTCTCAAGA AGGCTCAGCC AGCAGCACCA AAAGTACAGA ATTGCTACTA
    GGTGTTAAAA CAATTCCAGA TGATACACCA ATGTGCCGTA TACTCCTTCG TAAAGAAGTT
    CTGAGATTAG TCATTAATTT GAGTAGTTCA GTTTCAACTA AATGTCATGA AACTGGGCTT
    TTAACAATTA AAGAGAAGTA TCCTCAAACA TTTGATGACA TATGCCTTTA CTCTGAGGTT
    TCCCATTTGC TGTCACACTG CACATTTAGA CTTCCATGTC GGAGGTTCAT ACAAGAATTA
    TTTCAGGATG TACAGTTTTT ACAAATGCAT GAAGAAGCAG AGGCTGTCTT GGCAACACCA
    CCAAAGCAAA CTATAGTCGA TACATCTGCT GAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51387
    Clone ID Related Accession (Same CDS sequence) XM_010816809.2 , XM_010816809.3
    Accession Version XM_010816809.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGATC
    AATTTTAAAA AGAAGCATGT CGGGGGAATC AGGAGCTTAA GACCTACAAT AACAAACAAC
    TTTTTCAGGT TCATGAAAGC TTTAAGTTAT GCTTCGCTAG ATAAAGAAGA TTTATTAAGT
    CCTATTAACC AAAATACGCT GCAGCGCTCC TCCTCCGTGA GGTCCATGGT GTCCAGTGCG
    ACATACGGTA GCTCAGATGA CTATATTGGT CTTGCTCTTC CAGTAGATAT CAATGATATA
    TTCCAGGTAA AGGATATTCC TTATTTTCAA ACAAAAAACA TACCTCCACA TGATGATCGA
    GGTGCAAGAG TATTTGCCCA TGATGTTGGA GGTCTTCCAT CTGGAACTGG AGGTCTTGTA
    AAAAACTCTT TTCACTTGCT ACGACAGCAG ATGAGTCTTA CGGAAATAAT GAATTCAATC
    CATTCAGATG CTTCTCTGTT TTTAGAAAGT ACAGAAGACA CTGGACTACA GGAACATACA
    GATGATAACT GCCTTTATTG TGTCTGTATT GAAATTCTGG GTTTCCAGCC CAGTAACCAA
    CTGAGTGCAA TATGTAGTCA TTCAGACCTT CAAGACATTC CATATTCTGA TTGGTGTGAG
    CAGACTATCC ATAATCCCTT AGAAGTCGTT CCCTCTAAAT TTTCTGGCAT TTCTGGATGC
    AGTGATGGAG TGTCTCAAGA AGGCTCAGCC AGCAGCACCA AAAGTACAGA ATTGCTACTA
    GGTGTTAAAA CAATTCCAGA TGATACACCA ATGTGCCGTA TACTCCTTCG TAAAGAAGTT
    CTGAGATTAG TCATTAATTT GAGTAGTTCA GTTTCAACTA AATGTCATGA AACTGGGCTT
    TTAACAATTA AAGAGAAGTA TCCTCAAACA TTTGATGACA TATGCCTTTA CTCTGAGGTT
    TCCCATTTGC TGTCACACTG CACATTTAGA CTTCCATGTC GGAGGTTCAT ACAAGAATTA
    TTTCAGGATG TACAGTTTTT ACAAATGCAT GAAGAAGCAG AGGCTGTCTT GGCAACACCA
    CCAAAGCAAA CTATAGTCGA TACATCTGCT GAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815111.1
    CDS107..5305
    Translation

    Target ORF information:

    RefSeq Version XM_010816809.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCGA TCGGCCGCGG CCGCTCGCTG AAGAACCTCC GAATACGAGG GCGGAATGAC 
    AGCGGCGAGG AGAACGTCCC GCTGGATCTG ACCCGAGAAC CTTCTGATAA CTTAAGGGAG
    ATTCTCCAAA ATGTGGCCAA ATTGCAAGGA GTATCAAATA TGAGAAAGCT AGGCCATCTG
    AATAACTTTA CTAAGCTGCT TTGTGATATT GGCCACAGTG AAGAAAAACT GGGTTTTAAC
    TATGAGGATA TCATAATTTG CTTGCGGTTA GCTTTATTGA ATGAAGCAAA AGAAGTGCGG
    GCAGCAGGGC TTCGAGCTCT TCGGTATCTC ATCCAAGACT CCAGTATTCT GCAGAAGGTG
    CTAAAATTGA AAGTGGACTA TTTAATAGCT AGGTGCATTG ACATACAGCA GAGCAACGAG
    GTAGAGAGGA CACAAGCACT TCGATTAGTC AGAAAGATGA TTACTGTGAA TGCTTCCTTG
    TTTCCTAGCT CTGTGGCCAA CTCATTAATA GCAGTTGGAA ATGATGGACT TCAAGAAAGA
    GACAGAATGG TTCGAGCATG CATTGCCATT ATCTGTGAAC TAGCACTTCA GAATCCAGAG
    GTGGTTGCCC TTCGAGGTGG ACTGAACACC ATCTTGAAAA ATGTGATTGA TTGCCAATTA
    AGTCGAATAA ATGAGGCTCT AATTACTACA ATTTTGCACC TTCTTAATCA TCCAAAGACC
    CGACAGTATG TACGAGCTGA TGTCGAATTA GAGCGAATTT TAGCACCCTA TACCGATTTT
    CACTACAGAC ATAGTCCAGA TACAGCTGAA GGACAGCTAA AAGAAGACAG AGAAGCAAGG
    TTTCTAGCCA GTAAAATGGG AATCATAGCA ACATTCCGGT CATGGGCAGG TATTATTAAT
    TTATGTAAAC CTGGAAACTC TGGGATTCAG TCTCTAATTG GAGTACTTTG CATTCCAAAT
    ATGGAAATAA GGCGAGGTCT CCTTGAAGTG CTTTATGATA TATTTCGTCT TCCTCTACCT
    GTTGTGACTG AAGAGTTCAT AGAAGCACTA CTCAGTGTAG ATCCAGGTAG ATTCCAAGAC
    AGTTGGAGGC TTTCAGATGG CTTTGTAGCA GCTGAGGCAA AAACTATTCT TCCTCATCGT
    GCCAGATCCA GGCCAGACCT CATGGATAAT TATTTGGCAC TGATACTCTC AGCATTTATT
    CGTAATGGAC TTTTAGAAGG TTTGGTTGAG GTAATAACAA ACAGTGACGA CCATATCTCA
    GTTAGAGCCA CCATCCTTTT AGGAGAACTT TTACATATGG CAAACACAAT TCTTCCTCAT
    TCACATAGCC ATCATTTGCA CTGCCTGCCA ACCCTAATGA ATATGGCTGC ATCCTTTGAT
    ATCCCTAAGG AAAAGAGATT GCGAGCCAGT GCAGCTTTGA ACTGTTTAAA ACGCTTCCAT
    GAAATGAAAA AACGAGGACC TAAACCTTAT AGCCTTCATT TAGATCACAT TATTCAGAAA
    GCAATTGCAA CACATCAGAA ACGGGATCAG TATCTCCGAG CTCAGAAAGA TATATTTGTT
    CTTAAGGATA CAGAGGAAGC TCTTCTAATG AACCTTAGAG ATAGCCAAGT CCTTCAACAT
    AAAGAGAATC TTGAATGGAA TTGGAATCTT ATTGGGACCA TTCTTAAGTG GCCAAATGTA
    AATCTAAGAA ACTATAAAGA TGAGCAGTTG CACAGGTTTG TACGAAGACT ACTTTATTTT
    TACAAGCCCA GTAGTAAGTT ATACGCCAAT CTGGATCTGG ATTTTGCCAA GTCCAAGCAG
    CTCACAGTTG TGGGTTGTCA GTTTACAGAA TTTCTTCTTG AGTCTGAAGA GGATGGGCAG
    GGATACTTAG AAGATCTAGT AAAGGATATT GTTCAGTGGC TCAATGCCTC TTCTGGAATG
    AAACCTGAAA GAAGCCTTCA AAATAATGGT TTATTGACTA CTCTTAGTCA ACACTACTTT
    TTATTTATTG GAACACTTTC TTGCCACCCT CATGGCGTCA AAATGCTGGA GAAATGCAGC
    GTATTTCAAT GTTTACTTAA CCTTTGCTCC TTGAAAAACC AAGATCACTT GCTAAAACTG
    ACCGTTTCAA GCTTGGACTA TAGTAGAGAT GGTTTGGCCA GGGTCATCCT TTCCAAAATC
    TTAACAGCAG CCACTGATGC CTGCAGACTG TATGCAACAA AACATTTAAG AGTGTTATTG
    AGAGCTAATG TTGAATTCTT CAATAATTGG GGAATTGAAT TACTAGTGAC CCAGCTGCAT
    GATAAAAACA AAACGATTTC TTCTGAGGCC CTTGATATTC TCGATGAAGC ATGTGAAGAC
    AAGGCCAATC TTCATGCACT TATTCAGATG AAGCCAGCTT TATCTCACCT TGGGGACAAG
    GGTTTGCTTC TCCTCCTGAG ATTTCTCTCC ATTCCAAAAG GGTTTTCCTA TTTGAATGAA
    AGGGGTTATG TAACAAAACA GTTGGAAAAG TGGCACAAGG AATATAATTC AAAATACGTT
    GACTTGATTG AAGAACAGCT TAATGAAGCA CTTACCACTT ACCGGAAGCC AATTGATGGT
    GACAACTATG TTCGTCGGAG TAACCAAAGA TTACAGCGTC CTCATGTCTA CCTACCTGTA
    CATCTTTATG GACAACTGGT ACACCACAAA ACAGGCTGTC ATTTATTGGA AGTACAGAAT
    ATTATTACAG AACTCTGTCA CAATGTTCGT ACACCAGATT TGGATAAATG GGAAGAAATT
    AAAAAACTAA AAGCATCTCT TTGGGCCTTG GGAAATATTG GCTCATCTAA TTGGGGTCTC
    AATTTGCTAC AAGAAGAAAA CGTGATTCCA GATATCCTGA AACTTGCAAA ACAGTGTGAA
    GTTCTTTCAG TCAGAGGGAC CTGTGTATAT GTTCTTGGGC TCATAGCCAA AACCAAGCAA
    GGCTGTGATA TTCTAAAATG TCACAATTGG GATGCTGTAA GGCACAGTCG TAAACATTTG
    TGGCCAGTGG TTCCAGACGA TGTAGAACAA CTCTGTAATG AACTCTCATC TGTCCCGAGC
    ACCCTAAGTT TGAACTCGGA GTCAACAAGC TCTAGGCATA ATAGTGAGAG TGAATCTGCA
    CCGTCAAGTA TGTTCATCAT GGAGGATGAC CGGTTTGGCA GCAGCTCTAC CAGTACATTT
    TTCCTTGACA TCAATGAAGA TGCAGAGCCA GCATTTTATG ATCGACCTGG ACCTATAAAG
    GATAAAAATC CATTCCCTTT CTTTGCTTCT AGTAAACTTG TGAAGAATCG TATCTTAAAT
    TCTCTTACTT TGCCTAATAA AAAACATCGT AGCAGCAGTG ATCCAAAAGG AGGGAAACTG
    TCATCTGAAA ATAAGACAAG CAACAGGCGG ATCAGAACGC TTACAGAGCC CAGTGTTGAC
    TTTAATCATA GTGATGATTT CACACCCATA TCCACAGTAC AGAAAACATT ACAATTAGAA
    ACTTCATTTG TTGGGAATAA GCACATTGAA GACACTGGTA GTACTCCAAG TATTGGAGAG
    AATGACTTAA AATTCACCAA GAGTCTTGGT ACAGAGAATC ACAAAGAAAA CACAAGCCGA
    GAGAGATTAG TTGTAGAAAG TTCATCGAGC TCACATATGA AGATACGTAG CCAGAGTTTT
    AATACAGATA CTACAACAAG TGGCATAAGT TCAATGAGTT CAAGTCCTTC ACGAGAGACA
    GTAGGTGTAG ATGCTACGAC TGTGGACACA GACTGTGGAA GTATGAGTAC TGTGGTAAGT
    ACTAAAACTG TTAAGACAAG CCACTATTTG ACGCCACAGT CTAACCATCT CTCTCTCTCT
    AAATCAAGCT CAGTGTCCCT GGTGCCTCCA GGTTCTTCTC ACACACTTCC TAGAAGAGCA
    CAGTCCCTTA AAGCACCCTC TATTGCTACA ATTAAAAGTC TAGCAGACTA TAACTTTAGT
    TACACAAGTT CTAGAGATGC CTTTGGCTAC GCTACCCTGA AAAGATTACA ACAGCAAAGA
    ATGCATCCAT CCTTATCTCA TTCTGAAGCT TTGGCATCCC CAGCAAAAGA TGTGCTGTTT
    ACTGATACCA TCACCATGAA GGCCAACAGC TTTGAGTCCA GGTTAACACC AAGCAGGATC
    AATTTTAAAA AGAAGCATGT CGGGGGAATC AGGAGCTTAA GACCTACAAT AACAAACAAC
    TTTTTCAGGT TCATGAAAGC TTTAAGTTAT GCTTCGCTAG ATAAAGAAGA TTTATTAAGT
    CCTATTAACC AAAATACGCT GCAGCGCTCC TCCTCCGTGA GGTCCATGGT GTCCAGTGCG
    ACATACGGTA GCTCAGATGA CTATATTGGT CTTGCTCTTC CAGTAGATAT CAATGATATA
    TTCCAGGTAA AGGATATTCC TTATTTTCAA ACAAAAAACA TACCTCCACA TGATGATCGA
    GGTGCAAGAG TATTTGCCCA TGATGTTGGA GGTCTTCCAT CTGGAACTGG AGGTCTTGTA
    AAAAACTCTT TTCACTTGCT ACGACAGCAG ATGAGTCTTA CGGAAATAAT GAATTCAATC
    CATTCAGATG CTTCTCTGTT TTTAGAAAGT ACAGAAGACA CTGGACTACA GGAACATACA
    GATGATAACT GCCTTTATTG TGTCTGTATT GAAATTCTGG GTTTCCAGCC CAGTAACCAA
    CTGAGTGCAA TATGTAGTCA TTCAGACCTT CAAGACATTC CATATTCTGA TTGGTGTGAG
    CAGACTATCC ATAATCCCTT AGAAGTCGTT CCCTCTAAAT TTTCTGGCAT TTCTGGATGC
    AGTGATGGAG TGTCTCAAGA AGGCTCAGCC AGCAGCACCA AAAGTACAGA ATTGCTACTA
    GGTGTTAAAA CAATTCCAGA TGATACACCA ATGTGCCGTA TACTCCTTCG TAAAGAAGTT
    CTGAGATTAG TCATTAATTT GAGTAGTTCA GTTTCAACTA AATGTCATGA AACTGGGCTT
    TTAACAATTA AAGAGAAGTA TCCTCAAACA TTTGATGACA TATGCCTTTA CTCTGAGGTT
    TCCCATTTGC TGTCACACTG CACATTTAGA CTTCCATGTC GGAGGTTCAT ACAAGAATTA
    TTTCAGGATG TACAGTTTTT ACAAATGCAT GAAGAAGCAG AGGCTGTCTT GGCAACACCA
    CCAAAGCAAA CTATAGTCGA TACATCTGCT GAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51388
    Clone ID Related Accession (Same CDS sequence) XM_010816811.3 , XM_010816811.2
    Accession Version XM_010816811.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTACTG TGAATGCTTC CTTGTTTCCT AGCTCTGTGG CCAACTCATT AATAGCAGTT 
    GGAAATGATG GACTTCAAGA AAGAGACAGA ATGGTTCGAG CATGCATTGC CATTATCTGT
    GAACTAGCAC TTCAGAATCC AGAGGTGGTT GCCCTTCGAG GTGGACTGAA CACCATCTTG
    AAAAATGTGA TTGATTGCCA ATTAAGTCGA ATAAATGAGG CTCTAATTAC TACAATTTTG
    CACCTTCTTA ATCATCCAAA GACCCGACAG TATGTACGAG CTGATGTCGA ATTAGAGCGA
    ATTTTAGCAC CCTATACCGA TTTTCACTAC AGACATAGTC CAGATACAGC TGAAGGACAG
    CTAAAAGAAG ACAGAGAAGC AAGGTTTCTA GCCAGTAAAA TGGGAATCAT AGCAACATTC
    CGGTCATGGG CAGGTATTAT TAATTTATGT AAACCTGGAA ACTCTGGGAT TCAGTCTCTA
    ATTGGAGTAC TTTGCATTCC AAATATGGAA ATAAGGCGAG GTCTCCTTGA AGTGCTTTAT
    GATATATTTC GTCTTCCTCT ACCTGTTGTG ACTGAAGAGT TCATAGAAGC ACTACTCAGT
    GTAGATCCAG GTAGATTCCA AGACAGTTGG AGGCTTTCAG ATGGCTTTGT AGCAGCTGAG
    GCAAAAACTA TTCTTCCTCA TCGTGCCAGA TCCAGGCCAG ACCTCATGGA TAATTATTTG
    GCACTGATAC TCTCAGCATT TATTCGTAAT GGACTTTTAG AAGGTTTGGT TGAGGTAATA
    ACAAACAGTG ACGACCATAT CTCAGTTAGA GCCACCATCC TTTTAGGAGA ACTTTTACAT
    ATGGCAAACA CAATTCTTCC TCATTCACAT AGCCATCATT TGCACTGCCT GCCAACCCTA
    ATGAATATGG CTGCATCCTT TGATATCCCT AAGGAAAAGA GATTGCGAGC CAGTGCAGCT
    TTGAACTGTT TAAAACGCTT CCATGAAATG AAAAAACGAG GACCTAAACC TTATAGCCTT
    CATTTAGATC ACATTATTCA GAAAGCAATT GCAACACATC AGAAACGGGA TCAGTATCTC
    CGAGCTCAGA AAGATATATT TGTTCTTAAG GATACAGAGG AAGCTCTTCT AATGAACCTT
    AGAGATAGCC AAGTCCTTCA ACATAAAGAG AATCTTGAAT GGAATTGGAA TCTTATTGGG
    ACCATTCTTA AGTGGCCAAA TGTAAATCTA AGAAACTATA AAGATGAGCA GTTGCACAGG
    TTTGTACGAA GACTACTTTA TTTTTACAAG CCCAGTAGTA AGTTATACGC CAATCTGGAT
    CTGGATTTTG CCAAGTCCAA GCAGCTCACA GTTGTGGGTT GTCAGTTTAC AGAATTTCTT
    CTTGAGTCTG AAGAGGATGG GCAGGGATAC TTAGAAGATC TAGTAAAGGA TATTGTTCAG
    TGGCTCAATG CCTCTTCTGG AATGAAACCT GAAAGAAGCC TTCAAAATAA TGGTTTATTG
    ACTACTCTTA GTCAACACTA CTTTTTATTT ATTGGAACAC TTTCTTGCCA CCCTCATGGC
    GTCAAAATGC TGGAGAAATG CAGCGTATTT CAATGTTTAC TTAACCTTTG CTCCTTGAAA
    AACCAAGATC ACTTGCTAAA ACTGACCGTT TCAAGCTTGG ACTATAGTAG AGATGGTTTG
    GCCAGGGTCA TCCTTTCCAA AATCTTAACA GCAGCCACTG ATGCCTGCAG ACTGTATGCA
    ACAAAACATT TAAGAGTGTT ATTGAGAGCT AATGTTGAAT TCTTCAATAA TTGGGGAATT
    GAATTACTAG TGACCCAGCT GCATGATAAA AACAAAACGA TTTCTTCTGA GGCCCTTGAT
    ATTCTCGATG AAGCATGTGA AGACAAGGCC AATCTTCATG CACTTATTCA GATGAAGCCA
    GCTTTATCTC ACCTTGGGGA CAAGGGTTTG CTTCTCCTCC TGAGATTTCT CTCCATTCCA
    AAAGGGTTTT CCTATTTGAA TGAAAGGGGT TATGTAACAA AACAGTTGGA AAAGTGGCAC
    AAGGAATATA ATTCAAAATA CGTTGACTTG ATTGAAGAAC AGCTTAATGA AGCACTTACC
    ACTTACCGGA AGCCAATTGA TGGTGACAAC TATGTTCGTC GGAGTAACCA AAGATTACAG
    CGTCCTCATG TCTACCTACC TGTACATCTT TATGGACAAC TGGTACACCA CAAAACAGGC
    TGTCATTTAT TGGAAGTACA GAATATTATT ACAGAACTCT GTCACAATGT TCGTACACCA
    GATTTGGATA AATGGGAAGA AATTAAAAAA CTAAAAGCAT CTCTTTGGGC CTTGGGAAAT
    ATTGGCTCAT CTAATTGGGG TCTCAATTTG CTACAAGAAG AAAACGTGAT TCCAGATATC
    CTGAAACTTG CAAAACAGTG TGAAGTTCTT TCAGTCAGAG GGACCTGTGT ATATGTTCTT
    GGGCTCATAG CCAAAACCAA GCAAGGCTGT GATATTCTAA AATGTCACAA TTGGGATGCT
    GTAAGGCACA GTCGTAAACA TTTGTGGCCA GTGGTTCCAG ACGATGTAGA ACAACTCTGT
    AATGAACTCT CATCTGTCCC GAGCACCCTA AGTTTGAACT CGGAGTCAAC AAGCTCTAGG
    CATAATAGTG AGAGTGAATC TGCACCGTCA AGTATGTTCA TCATGGAGGA TGACCGGTTT
    GGCAGCAGCT CTACCAGTAC ATTTTTCCTT GACATCAATG AAGATGCAGA GCCAGCATTT
    TATGATCGAC CTGGACCTAT AAAGGATAAA AATCCATTCC CTTTCTTTGC TTCTAGTAAA
    CTTGTGAAGA ATCGTATCTT AAATTCTCTT ACTTTGCCTA ATAAAAAACA TCGTAGCAGC
    AGTGATCCAA AAGGAGGGAA ACTGTCATCT GAAAATAAGA CAAGCAACAG GCGGATCAGA
    ACGCTTACAG AGCCCAGTGT TGACTTTAAT CATAGTGATG ATTTCACACC CATATCCACA
    GTACAGAAAA CATTACAATT AGAAACTTCA TTTGTTGGGA ATAAGCACAT TGAAGACACT
    GGTAGTACTC CAAGTATTGG AGAGAATGAC TTAAAATTCA CCAAGAGTCT TGGTACAGAG
    AATCACAAAG AAAACACAAG CCGAGAGAGA TTAGTTGTAG AAAGTTCATC GAGCTCACAT
    ATGAAGATAC GTAGCCAGAG TTTTAATACA GATACTACAA CAAGTGGCAT AAGTTCAATG
    AGTTCAAGTC CTTCACGAGA GACAGTAGGT GTAGATGCTA CGACTGTGGA CACAGACTGT
    GGAAGTATGA GTACTGTGGT AAGTACTAAA ACTGTTAAGA CAAGCCACTA TTTGACGCCA
    CAGTCTAACC ATCTCTCTCT CTCTAAATCA AGCTCAGTGT CCCTGGTGCC TCCAGGTTCT
    TCTCACACAC TTCCTAGAAG AGCACAGTCC CTTAAAGCAC CCTCTATTGC TACAATTAAA
    AGTCTAGCAG ACTATAACTT TAGTTACACA AGTTCTAGAG ATGCCTTTGG CTACGCTACC
    CTGAAAAGAT TACAACAGCA AAGAATGCAT CCATCCTTAT CTCATTCTGA AGCTTTGGCA
    TCCCCAGCAA AAGATGTGCT GTTTACTGAT ACCATCACCA TGAAGGCCAA CAGCTTTGAG
    TCCAGGTTAA CACCAAGCAG GATCAATTTT AAAAAGAAGC ATGTCGGGGG AATCAGGAGC
    TTAAGACCTA CAATAACAAA CAACTTTTTC AGGTTCATGA AAGCTTTAAG TTATGCTTCG
    CTAGATAAAG AAGATTTATT AAGTCCTATT AACCAAAATA CGCTGCAGCG CTCCTCCTCC
    GTGAGGTCCA TGGTGTCCAG TGCGACATAC GGTAGCTCAG ATGACTATAT TGGTCTTGCT
    CTTCCAGTAG ATATCAATGA TATATTCCAG GTAAAGGATA TTCCTTATTT TCAAACAAAA
    AACATACCTC CACATGATGA TCGAGGTGCA AGAGTATTTG CCCATGATGT TGGAGGTCTT
    CCATCTGGAA CTGGAGGTCT TGTAAAAAAC TCTTTTCACT TGCTACGACA GCAGATGAGT
    CTTACGGAAA TAATGAATTC AATCCATTCA GATGCTTCTC TGTTTTTAGA AAGTACAGAA
    GACACTGGAC TACAGGAACA TACAGATGAT AACTGCCTTT ATTGTGTCTG TATTGAAATT
    CTGGGTTTCC AGCCCAGTAA CCAACTGAGT GCAATATGTA GTCATTCAGA CCTTCAAGAC
    ATTCCATATT CTGATTGGTG TGAGCAGACT ATCCATAATC CCTTAGAAGT CGTTCCCTCT
    AAATTTTCTG GCATTTCTGG ATGCAGTGAT GGAGTGTCTC AAGAAGGCTC AGCCAGCAGC
    ACCAAAAGTA CAGAATTGCT ACTAGGTGTT AAAACAATTC CAGATGATAC ACCAATGTGC
    CGTATACTCC TTCGTAAAGA AGTTCTGAGA TTAGTCATTA ATTTGAGTAG TTCAGTTTCA
    ACTAAATGTC ATGAAACTGG GCTTTTAACA ATTAAAGAGA AGTATCCTCA AACATTTGAT
    GACATATGCC TTTACTCTGA GGTTTCCCAT TTGCTGTCAC ACTGCACATT TAGACTTCCA
    TGTCGGAGGT TCATACAAGA ATTATTTCAG GATGTACAGT TTTTACAAAT GCATGAAGAA
    GCAGAGGCTG TCTTGGCAAC ACCACCAAAG CAAACTATAG TCGATACATC TGCTGAATCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815113.1
    CDS163..4905
    Translation

    Target ORF information:

    RefSeq Version XM_010816811.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816811.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTACTG TGAATGCTTC CTTGTTTCCT AGCTCTGTGG CCAACTCATT AATAGCAGTT 
    GGAAATGATG GACTTCAAGA AAGAGACAGA ATGGTTCGAG CATGCATTGC CATTATCTGT
    GAACTAGCAC TTCAGAATCC AGAGGTGGTT GCCCTTCGAG GTGGACTGAA CACCATCTTG
    AAAAATGTGA TTGATTGCCA ATTAAGTCGA ATAAATGAGG CTCTAATTAC TACAATTTTG
    CACCTTCTTA ATCATCCAAA GACCCGACAG TATGTACGAG CTGATGTCGA ATTAGAGCGA
    ATTTTAGCAC CCTATACCGA TTTTCACTAC AGACATAGTC CAGATACAGC TGAAGGACAG
    CTAAAAGAAG ACAGAGAAGC AAGGTTTCTA GCCAGTAAAA TGGGAATCAT AGCAACATTC
    CGGTCATGGG CAGGTATTAT TAATTTATGT AAACCTGGAA ACTCTGGGAT TCAGTCTCTA
    ATTGGAGTAC TTTGCATTCC AAATATGGAA ATAAGGCGAG GTCTCCTTGA AGTGCTTTAT
    GATATATTTC GTCTTCCTCT ACCTGTTGTG ACTGAAGAGT TCATAGAAGC ACTACTCAGT
    GTAGATCCAG GTAGATTCCA AGACAGTTGG AGGCTTTCAG ATGGCTTTGT AGCAGCTGAG
    GCAAAAACTA TTCTTCCTCA TCGTGCCAGA TCCAGGCCAG ACCTCATGGA TAATTATTTG
    GCACTGATAC TCTCAGCATT TATTCGTAAT GGACTTTTAG AAGGTTTGGT TGAGGTAATA
    ACAAACAGTG ACGACCATAT CTCAGTTAGA GCCACCATCC TTTTAGGAGA ACTTTTACAT
    ATGGCAAACA CAATTCTTCC TCATTCACAT AGCCATCATT TGCACTGCCT GCCAACCCTA
    ATGAATATGG CTGCATCCTT TGATATCCCT AAGGAAAAGA GATTGCGAGC CAGTGCAGCT
    TTGAACTGTT TAAAACGCTT CCATGAAATG AAAAAACGAG GACCTAAACC TTATAGCCTT
    CATTTAGATC ACATTATTCA GAAAGCAATT GCAACACATC AGAAACGGGA TCAGTATCTC
    CGAGCTCAGA AAGATATATT TGTTCTTAAG GATACAGAGG AAGCTCTTCT AATGAACCTT
    AGAGATAGCC AAGTCCTTCA ACATAAAGAG AATCTTGAAT GGAATTGGAA TCTTATTGGG
    ACCATTCTTA AGTGGCCAAA TGTAAATCTA AGAAACTATA AAGATGAGCA GTTGCACAGG
    TTTGTACGAA GACTACTTTA TTTTTACAAG CCCAGTAGTA AGTTATACGC CAATCTGGAT
    CTGGATTTTG CCAAGTCCAA GCAGCTCACA GTTGTGGGTT GTCAGTTTAC AGAATTTCTT
    CTTGAGTCTG AAGAGGATGG GCAGGGATAC TTAGAAGATC TAGTAAAGGA TATTGTTCAG
    TGGCTCAATG CCTCTTCTGG AATGAAACCT GAAAGAAGCC TTCAAAATAA TGGTTTATTG
    ACTACTCTTA GTCAACACTA CTTTTTATTT ATTGGAACAC TTTCTTGCCA CCCTCATGGC
    GTCAAAATGC TGGAGAAATG CAGCGTATTT CAATGTTTAC TTAACCTTTG CTCCTTGAAA
    AACCAAGATC ACTTGCTAAA ACTGACCGTT TCAAGCTTGG ACTATAGTAG AGATGGTTTG
    GCCAGGGTCA TCCTTTCCAA AATCTTAACA GCAGCCACTG ATGCCTGCAG ACTGTATGCA
    ACAAAACATT TAAGAGTGTT ATTGAGAGCT AATGTTGAAT TCTTCAATAA TTGGGGAATT
    GAATTACTAG TGACCCAGCT GCATGATAAA AACAAAACGA TTTCTTCTGA GGCCCTTGAT
    ATTCTCGATG AAGCATGTGA AGACAAGGCC AATCTTCATG CACTTATTCA GATGAAGCCA
    GCTTTATCTC ACCTTGGGGA CAAGGGTTTG CTTCTCCTCC TGAGATTTCT CTCCATTCCA
    AAAGGGTTTT CCTATTTGAA TGAAAGGGGT TATGTAACAA AACAGTTGGA AAAGTGGCAC
    AAGGAATATA ATTCAAAATA CGTTGACTTG ATTGAAGAAC AGCTTAATGA AGCACTTACC
    ACTTACCGGA AGCCAATTGA TGGTGACAAC TATGTTCGTC GGAGTAACCA AAGATTACAG
    CGTCCTCATG TCTACCTACC TGTACATCTT TATGGACAAC TGGTACACCA CAAAACAGGC
    TGTCATTTAT TGGAAGTACA GAATATTATT ACAGAACTCT GTCACAATGT TCGTACACCA
    GATTTGGATA AATGGGAAGA AATTAAAAAA CTAAAAGCAT CTCTTTGGGC CTTGGGAAAT
    ATTGGCTCAT CTAATTGGGG TCTCAATTTG CTACAAGAAG AAAACGTGAT TCCAGATATC
    CTGAAACTTG CAAAACAGTG TGAAGTTCTT TCAGTCAGAG GGACCTGTGT ATATGTTCTT
    GGGCTCATAG CCAAAACCAA GCAAGGCTGT GATATTCTAA AATGTCACAA TTGGGATGCT
    GTAAGGCACA GTCGTAAACA TTTGTGGCCA GTGGTTCCAG ACGATGTAGA ACAACTCTGT
    AATGAACTCT CATCTGTCCC GAGCACCCTA AGTTTGAACT CGGAGTCAAC AAGCTCTAGG
    CATAATAGTG AGAGTGAATC TGCACCGTCA AGTATGTTCA TCATGGAGGA TGACCGGTTT
    GGCAGCAGCT CTACCAGTAC ATTTTTCCTT GACATCAATG AAGATGCAGA GCCAGCATTT
    TATGATCGAC CTGGACCTAT AAAGGATAAA AATCCATTCC CTTTCTTTGC TTCTAGTAAA
    CTTGTGAAGA ATCGTATCTT AAATTCTCTT ACTTTGCCTA ATAAAAAACA TCGTAGCAGC
    AGTGATCCAA AAGGAGGGAA ACTGTCATCT GAAAATAAGA CAAGCAACAG GCGGATCAGA
    ACGCTTACAG AGCCCAGTGT TGACTTTAAT CATAGTGATG ATTTCACACC CATATCCACA
    GTACAGAAAA CATTACAATT AGAAACTTCA TTTGTTGGGA ATAAGCACAT TGAAGACACT
    GGTAGTACTC CAAGTATTGG AGAGAATGAC TTAAAATTCA CCAAGAGTCT TGGTACAGAG
    AATCACAAAG AAAACACAAG CCGAGAGAGA TTAGTTGTAG AAAGTTCATC GAGCTCACAT
    ATGAAGATAC GTAGCCAGAG TTTTAATACA GATACTACAA CAAGTGGCAT AAGTTCAATG
    AGTTCAAGTC CTTCACGAGA GACAGTAGGT GTAGATGCTA CGACTGTGGA CACAGACTGT
    GGAAGTATGA GTACTGTGGT AAGTACTAAA ACTGTTAAGA CAAGCCACTA TTTGACGCCA
    CAGTCTAACC ATCTCTCTCT CTCTAAATCA AGCTCAGTGT CCCTGGTGCC TCCAGGTTCT
    TCTCACACAC TTCCTAGAAG AGCACAGTCC CTTAAAGCAC CCTCTATTGC TACAATTAAA
    AGTCTAGCAG ACTATAACTT TAGTTACACA AGTTCTAGAG ATGCCTTTGG CTACGCTACC
    CTGAAAAGAT TACAACAGCA AAGAATGCAT CCATCCTTAT CTCATTCTGA AGCTTTGGCA
    TCCCCAGCAA AAGATGTGCT GTTTACTGAT ACCATCACCA TGAAGGCCAA CAGCTTTGAG
    TCCAGGTTAA CACCAAGCAG GATCAATTTT AAAAAGAAGC ATGTCGGGGG AATCAGGAGC
    TTAAGACCTA CAATAACAAA CAACTTTTTC AGGTTCATGA AAGCTTTAAG TTATGCTTCG
    CTAGATAAAG AAGATTTATT AAGTCCTATT AACCAAAATA CGCTGCAGCG CTCCTCCTCC
    GTGAGGTCCA TGGTGTCCAG TGCGACATAC GGTAGCTCAG ATGACTATAT TGGTCTTGCT
    CTTCCAGTAG ATATCAATGA TATATTCCAG GTAAAGGATA TTCCTTATTT TCAAACAAAA
    AACATACCTC CACATGATGA TCGAGGTGCA AGAGTATTTG CCCATGATGT TGGAGGTCTT
    CCATCTGGAA CTGGAGGTCT TGTAAAAAAC TCTTTTCACT TGCTACGACA GCAGATGAGT
    CTTACGGAAA TAATGAATTC AATCCATTCA GATGCTTCTC TGTTTTTAGA AAGTACAGAA
    GACACTGGAC TACAGGAACA TACAGATGAT AACTGCCTTT ATTGTGTCTG TATTGAAATT
    CTGGGTTTCC AGCCCAGTAA CCAACTGAGT GCAATATGTA GTCATTCAGA CCTTCAAGAC
    ATTCCATATT CTGATTGGTG TGAGCAGACT ATCCATAATC CCTTAGAAGT CGTTCCCTCT
    AAATTTTCTG GCATTTCTGG ATGCAGTGAT GGAGTGTCTC AAGAAGGCTC AGCCAGCAGC
    ACCAAAAGTA CAGAATTGCT ACTAGGTGTT AAAACAATTC CAGATGATAC ACCAATGTGC
    CGTATACTCC TTCGTAAAGA AGTTCTGAGA TTAGTCATTA ATTTGAGTAG TTCAGTTTCA
    ACTAAATGTC ATGAAACTGG GCTTTTAACA ATTAAAGAGA AGTATCCTCA AACATTTGAT
    GACATATGCC TTTACTCTGA GGTTTCCCAT TTGCTGTCAC ACTGCACATT TAGACTTCCA
    TGTCGGAGGT TCATACAAGA ATTATTTCAG GATGTACAGT TTTTACAAAT GCATGAAGAA
    GCAGAGGCTG TCTTGGCAAC ACCACCAAAG CAAACTATAG TCGATACATC TGCTGAATCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51388
    Clone ID Related Accession (Same CDS sequence) XM_010816811.3 , XM_010816811.2
    Accession Version XM_010816811.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rapamycin-insensitive companion of mTOR isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTACTG TGAATGCTTC CTTGTTTCCT AGCTCTGTGG CCAACTCATT AATAGCAGTT 
    GGAAATGATG GACTTCAAGA AAGAGACAGA ATGGTTCGAG CATGCATTGC CATTATCTGT
    GAACTAGCAC TTCAGAATCC AGAGGTGGTT GCCCTTCGAG GTGGACTGAA CACCATCTTG
    AAAAATGTGA TTGATTGCCA ATTAAGTCGA ATAAATGAGG CTCTAATTAC TACAATTTTG
    CACCTTCTTA ATCATCCAAA GACCCGACAG TATGTACGAG CTGATGTCGA ATTAGAGCGA
    ATTTTAGCAC CCTATACCGA TTTTCACTAC AGACATAGTC CAGATACAGC TGAAGGACAG
    CTAAAAGAAG ACAGAGAAGC AAGGTTTCTA GCCAGTAAAA TGGGAATCAT AGCAACATTC
    CGGTCATGGG CAGGTATTAT TAATTTATGT AAACCTGGAA ACTCTGGGAT TCAGTCTCTA
    ATTGGAGTAC TTTGCATTCC AAATATGGAA ATAAGGCGAG GTCTCCTTGA AGTGCTTTAT
    GATATATTTC GTCTTCCTCT ACCTGTTGTG ACTGAAGAGT TCATAGAAGC ACTACTCAGT
    GTAGATCCAG GTAGATTCCA AGACAGTTGG AGGCTTTCAG ATGGCTTTGT AGCAGCTGAG
    GCAAAAACTA TTCTTCCTCA TCGTGCCAGA TCCAGGCCAG ACCTCATGGA TAATTATTTG
    GCACTGATAC TCTCAGCATT TATTCGTAAT GGACTTTTAG AAGGTTTGGT TGAGGTAATA
    ACAAACAGTG ACGACCATAT CTCAGTTAGA GCCACCATCC TTTTAGGAGA ACTTTTACAT
    ATGGCAAACA CAATTCTTCC TCATTCACAT AGCCATCATT TGCACTGCCT GCCAACCCTA
    ATGAATATGG CTGCATCCTT TGATATCCCT AAGGAAAAGA GATTGCGAGC CAGTGCAGCT
    TTGAACTGTT TAAAACGCTT CCATGAAATG AAAAAACGAG GACCTAAACC TTATAGCCTT
    CATTTAGATC ACATTATTCA GAAAGCAATT GCAACACATC AGAAACGGGA TCAGTATCTC
    CGAGCTCAGA AAGATATATT TGTTCTTAAG GATACAGAGG AAGCTCTTCT AATGAACCTT
    AGAGATAGCC AAGTCCTTCA ACATAAAGAG AATCTTGAAT GGAATTGGAA TCTTATTGGG
    ACCATTCTTA AGTGGCCAAA TGTAAATCTA AGAAACTATA AAGATGAGCA GTTGCACAGG
    TTTGTACGAA GACTACTTTA TTTTTACAAG CCCAGTAGTA AGTTATACGC CAATCTGGAT
    CTGGATTTTG CCAAGTCCAA GCAGCTCACA GTTGTGGGTT GTCAGTTTAC AGAATTTCTT
    CTTGAGTCTG AAGAGGATGG GCAGGGATAC TTAGAAGATC TAGTAAAGGA TATTGTTCAG
    TGGCTCAATG CCTCTTCTGG AATGAAACCT GAAAGAAGCC TTCAAAATAA TGGTTTATTG
    ACTACTCTTA GTCAACACTA CTTTTTATTT ATTGGAACAC TTTCTTGCCA CCCTCATGGC
    GTCAAAATGC TGGAGAAATG CAGCGTATTT CAATGTTTAC TTAACCTTTG CTCCTTGAAA
    AACCAAGATC ACTTGCTAAA ACTGACCGTT TCAAGCTTGG ACTATAGTAG AGATGGTTTG
    GCCAGGGTCA TCCTTTCCAA AATCTTAACA GCAGCCACTG ATGCCTGCAG ACTGTATGCA
    ACAAAACATT TAAGAGTGTT ATTGAGAGCT AATGTTGAAT TCTTCAATAA TTGGGGAATT
    GAATTACTAG TGACCCAGCT GCATGATAAA AACAAAACGA TTTCTTCTGA GGCCCTTGAT
    ATTCTCGATG AAGCATGTGA AGACAAGGCC AATCTTCATG CACTTATTCA GATGAAGCCA
    GCTTTATCTC ACCTTGGGGA CAAGGGTTTG CTTCTCCTCC TGAGATTTCT CTCCATTCCA
    AAAGGGTTTT CCTATTTGAA TGAAAGGGGT TATGTAACAA AACAGTTGGA AAAGTGGCAC
    AAGGAATATA ATTCAAAATA CGTTGACTTG ATTGAAGAAC AGCTTAATGA AGCACTTACC
    ACTTACCGGA AGCCAATTGA TGGTGACAAC TATGTTCGTC GGAGTAACCA AAGATTACAG
    CGTCCTCATG TCTACCTACC TGTACATCTT TATGGACAAC TGGTACACCA CAAAACAGGC
    TGTCATTTAT TGGAAGTACA GAATATTATT ACAGAACTCT GTCACAATGT TCGTACACCA
    GATTTGGATA AATGGGAAGA AATTAAAAAA CTAAAAGCAT CTCTTTGGGC CTTGGGAAAT
    ATTGGCTCAT CTAATTGGGG TCTCAATTTG CTACAAGAAG AAAACGTGAT TCCAGATATC
    CTGAAACTTG CAAAACAGTG TGAAGTTCTT TCAGTCAGAG GGACCTGTGT ATATGTTCTT
    GGGCTCATAG CCAAAACCAA GCAAGGCTGT GATATTCTAA AATGTCACAA TTGGGATGCT
    GTAAGGCACA GTCGTAAACA TTTGTGGCCA GTGGTTCCAG ACGATGTAGA ACAACTCTGT
    AATGAACTCT CATCTGTCCC GAGCACCCTA AGTTTGAACT CGGAGTCAAC AAGCTCTAGG
    CATAATAGTG AGAGTGAATC TGCACCGTCA AGTATGTTCA TCATGGAGGA TGACCGGTTT
    GGCAGCAGCT CTACCAGTAC ATTTTTCCTT GACATCAATG AAGATGCAGA GCCAGCATTT
    TATGATCGAC CTGGACCTAT AAAGGATAAA AATCCATTCC CTTTCTTTGC TTCTAGTAAA
    CTTGTGAAGA ATCGTATCTT AAATTCTCTT ACTTTGCCTA ATAAAAAACA TCGTAGCAGC
    AGTGATCCAA AAGGAGGGAA ACTGTCATCT GAAAATAAGA CAAGCAACAG GCGGATCAGA
    ACGCTTACAG AGCCCAGTGT TGACTTTAAT CATAGTGATG ATTTCACACC CATATCCACA
    GTACAGAAAA CATTACAATT AGAAACTTCA TTTGTTGGGA ATAAGCACAT TGAAGACACT
    GGTAGTACTC CAAGTATTGG AGAGAATGAC TTAAAATTCA CCAAGAGTCT TGGTACAGAG
    AATCACAAAG AAAACACAAG CCGAGAGAGA TTAGTTGTAG AAAGTTCATC GAGCTCACAT
    ATGAAGATAC GTAGCCAGAG TTTTAATACA GATACTACAA CAAGTGGCAT AAGTTCAATG
    AGTTCAAGTC CTTCACGAGA GACAGTAGGT GTAGATGCTA CGACTGTGGA CACAGACTGT
    GGAAGTATGA GTACTGTGGT AAGTACTAAA ACTGTTAAGA CAAGCCACTA TTTGACGCCA
    CAGTCTAACC ATCTCTCTCT CTCTAAATCA AGCTCAGTGT CCCTGGTGCC TCCAGGTTCT
    TCTCACACAC TTCCTAGAAG AGCACAGTCC CTTAAAGCAC CCTCTATTGC TACAATTAAA
    AGTCTAGCAG ACTATAACTT TAGTTACACA AGTTCTAGAG ATGCCTTTGG CTACGCTACC
    CTGAAAAGAT TACAACAGCA AAGAATGCAT CCATCCTTAT CTCATTCTGA AGCTTTGGCA
    TCCCCAGCAA AAGATGTGCT GTTTACTGAT ACCATCACCA TGAAGGCCAA CAGCTTTGAG
    TCCAGGTTAA CACCAAGCAG GATCAATTTT AAAAAGAAGC ATGTCGGGGG AATCAGGAGC
    TTAAGACCTA CAATAACAAA CAACTTTTTC AGGTTCATGA AAGCTTTAAG TTATGCTTCG
    CTAGATAAAG AAGATTTATT AAGTCCTATT AACCAAAATA CGCTGCAGCG CTCCTCCTCC
    GTGAGGTCCA TGGTGTCCAG TGCGACATAC GGTAGCTCAG ATGACTATAT TGGTCTTGCT
    CTTCCAGTAG ATATCAATGA TATATTCCAG GTAAAGGATA TTCCTTATTT TCAAACAAAA
    AACATACCTC CACATGATGA TCGAGGTGCA AGAGTATTTG CCCATGATGT TGGAGGTCTT
    CCATCTGGAA CTGGAGGTCT TGTAAAAAAC TCTTTTCACT TGCTACGACA GCAGATGAGT
    CTTACGGAAA TAATGAATTC AATCCATTCA GATGCTTCTC TGTTTTTAGA AAGTACAGAA
    GACACTGGAC TACAGGAACA TACAGATGAT AACTGCCTTT ATTGTGTCTG TATTGAAATT
    CTGGGTTTCC AGCCCAGTAA CCAACTGAGT GCAATATGTA GTCATTCAGA CCTTCAAGAC
    ATTCCATATT CTGATTGGTG TGAGCAGACT ATCCATAATC CCTTAGAAGT CGTTCCCTCT
    AAATTTTCTG GCATTTCTGG ATGCAGTGAT GGAGTGTCTC AAGAAGGCTC AGCCAGCAGC
    ACCAAAAGTA CAGAATTGCT ACTAGGTGTT AAAACAATTC CAGATGATAC ACCAATGTGC
    CGTATACTCC TTCGTAAAGA AGTTCTGAGA TTAGTCATTA ATTTGAGTAG TTCAGTTTCA
    ACTAAATGTC ATGAAACTGG GCTTTTAACA ATTAAAGAGA AGTATCCTCA AACATTTGAT
    GACATATGCC TTTACTCTGA GGTTTCCCAT TTGCTGTCAC ACTGCACATT TAGACTTCCA
    TGTCGGAGGT TCATACAAGA ATTATTTCAG GATGTACAGT TTTTACAAAT GCATGAAGAA
    GCAGAGGCTG TCTTGGCAAC ACCACCAAAG CAAACTATAG TCGATACATC TGCTGAATCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815113.1
    CDS126..4868
    Translation

    Target ORF information:

    RefSeq Version XM_010816811.2
    Organism Bos taurus(cattle)
    Definition Bos taurus RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816811.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTACTG TGAATGCTTC CTTGTTTCCT AGCTCTGTGG CCAACTCATT AATAGCAGTT 
    GGAAATGATG GACTTCAAGA AAGAGACAGA ATGGTTCGAG CATGCATTGC CATTATCTGT
    GAACTAGCAC TTCAGAATCC AGAGGTGGTT GCCCTTCGAG GTGGACTGAA CACCATCTTG
    AAAAATGTGA TTGATTGCCA ATTAAGTCGA ATAAATGAGG CTCTAATTAC TACAATTTTG
    CACCTTCTTA ATCATCCAAA GACCCGACAG TATGTACGAG CTGATGTCGA ATTAGAGCGA
    ATTTTAGCAC CCTATACCGA TTTTCACTAC AGACATAGTC CAGATACAGC TGAAGGACAG
    CTAAAAGAAG ACAGAGAAGC AAGGTTTCTA GCCAGTAAAA TGGGAATCAT AGCAACATTC
    CGGTCATGGG CAGGTATTAT TAATTTATGT AAACCTGGAA ACTCTGGGAT TCAGTCTCTA
    ATTGGAGTAC TTTGCATTCC AAATATGGAA ATAAGGCGAG GTCTCCTTGA AGTGCTTTAT
    GATATATTTC GTCTTCCTCT ACCTGTTGTG ACTGAAGAGT TCATAGAAGC ACTACTCAGT
    GTAGATCCAG GTAGATTCCA AGACAGTTGG AGGCTTTCAG ATGGCTTTGT AGCAGCTGAG
    GCAAAAACTA TTCTTCCTCA TCGTGCCAGA TCCAGGCCAG ACCTCATGGA TAATTATTTG
    GCACTGATAC TCTCAGCATT TATTCGTAAT GGACTTTTAG AAGGTTTGGT TGAGGTAATA
    ACAAACAGTG ACGACCATAT CTCAGTTAGA GCCACCATCC TTTTAGGAGA ACTTTTACAT
    ATGGCAAACA CAATTCTTCC TCATTCACAT AGCCATCATT TGCACTGCCT GCCAACCCTA
    ATGAATATGG CTGCATCCTT TGATATCCCT AAGGAAAAGA GATTGCGAGC CAGTGCAGCT
    TTGAACTGTT TAAAACGCTT CCATGAAATG AAAAAACGAG GACCTAAACC TTATAGCCTT
    CATTTAGATC ACATTATTCA GAAAGCAATT GCAACACATC AGAAACGGGA TCAGTATCTC
    CGAGCTCAGA AAGATATATT TGTTCTTAAG GATACAGAGG AAGCTCTTCT AATGAACCTT
    AGAGATAGCC AAGTCCTTCA ACATAAAGAG AATCTTGAAT GGAATTGGAA TCTTATTGGG
    ACCATTCTTA AGTGGCCAAA TGTAAATCTA AGAAACTATA AAGATGAGCA GTTGCACAGG
    TTTGTACGAA GACTACTTTA TTTTTACAAG CCCAGTAGTA AGTTATACGC CAATCTGGAT
    CTGGATTTTG CCAAGTCCAA GCAGCTCACA GTTGTGGGTT GTCAGTTTAC AGAATTTCTT
    CTTGAGTCTG AAGAGGATGG GCAGGGATAC TTAGAAGATC TAGTAAAGGA TATTGTTCAG
    TGGCTCAATG CCTCTTCTGG AATGAAACCT GAAAGAAGCC TTCAAAATAA TGGTTTATTG
    ACTACTCTTA GTCAACACTA CTTTTTATTT ATTGGAACAC TTTCTTGCCA CCCTCATGGC
    GTCAAAATGC TGGAGAAATG CAGCGTATTT CAATGTTTAC TTAACCTTTG CTCCTTGAAA
    AACCAAGATC ACTTGCTAAA ACTGACCGTT TCAAGCTTGG ACTATAGTAG AGATGGTTTG
    GCCAGGGTCA TCCTTTCCAA AATCTTAACA GCAGCCACTG ATGCCTGCAG ACTGTATGCA
    ACAAAACATT TAAGAGTGTT ATTGAGAGCT AATGTTGAAT TCTTCAATAA TTGGGGAATT
    GAATTACTAG TGACCCAGCT GCATGATAAA AACAAAACGA TTTCTTCTGA GGCCCTTGAT
    ATTCTCGATG AAGCATGTGA AGACAAGGCC AATCTTCATG CACTTATTCA GATGAAGCCA
    GCTTTATCTC ACCTTGGGGA CAAGGGTTTG CTTCTCCTCC TGAGATTTCT CTCCATTCCA
    AAAGGGTTTT CCTATTTGAA TGAAAGGGGT TATGTAACAA AACAGTTGGA AAAGTGGCAC
    AAGGAATATA ATTCAAAATA CGTTGACTTG ATTGAAGAAC AGCTTAATGA AGCACTTACC
    ACTTACCGGA AGCCAATTGA TGGTGACAAC TATGTTCGTC GGAGTAACCA AAGATTACAG
    CGTCCTCATG TCTACCTACC TGTACATCTT TATGGACAAC TGGTACACCA CAAAACAGGC
    TGTCATTTAT TGGAAGTACA GAATATTATT ACAGAACTCT GTCACAATGT TCGTACACCA
    GATTTGGATA AATGGGAAGA AATTAAAAAA CTAAAAGCAT CTCTTTGGGC CTTGGGAAAT
    ATTGGCTCAT CTAATTGGGG TCTCAATTTG CTACAAGAAG AAAACGTGAT TCCAGATATC
    CTGAAACTTG CAAAACAGTG TGAAGTTCTT TCAGTCAGAG GGACCTGTGT ATATGTTCTT
    GGGCTCATAG CCAAAACCAA GCAAGGCTGT GATATTCTAA AATGTCACAA TTGGGATGCT
    GTAAGGCACA GTCGTAAACA TTTGTGGCCA GTGGTTCCAG ACGATGTAGA ACAACTCTGT
    AATGAACTCT CATCTGTCCC GAGCACCCTA AGTTTGAACT CGGAGTCAAC AAGCTCTAGG
    CATAATAGTG AGAGTGAATC TGCACCGTCA AGTATGTTCA TCATGGAGGA TGACCGGTTT
    GGCAGCAGCT CTACCAGTAC ATTTTTCCTT GACATCAATG AAGATGCAGA GCCAGCATTT
    TATGATCGAC CTGGACCTAT AAAGGATAAA AATCCATTCC CTTTCTTTGC TTCTAGTAAA
    CTTGTGAAGA ATCGTATCTT AAATTCTCTT ACTTTGCCTA ATAAAAAACA TCGTAGCAGC
    AGTGATCCAA AAGGAGGGAA ACTGTCATCT GAAAATAAGA CAAGCAACAG GCGGATCAGA
    ACGCTTACAG AGCCCAGTGT TGACTTTAAT CATAGTGATG ATTTCACACC CATATCCACA
    GTACAGAAAA CATTACAATT AGAAACTTCA TTTGTTGGGA ATAAGCACAT TGAAGACACT
    GGTAGTACTC CAAGTATTGG AGAGAATGAC TTAAAATTCA CCAAGAGTCT TGGTACAGAG
    AATCACAAAG AAAACACAAG CCGAGAGAGA TTAGTTGTAG AAAGTTCATC GAGCTCACAT
    ATGAAGATAC GTAGCCAGAG TTTTAATACA GATACTACAA CAAGTGGCAT AAGTTCAATG
    AGTTCAAGTC CTTCACGAGA GACAGTAGGT GTAGATGCTA CGACTGTGGA CACAGACTGT
    GGAAGTATGA GTACTGTGGT AAGTACTAAA ACTGTTAAGA CAAGCCACTA TTTGACGCCA
    CAGTCTAACC ATCTCTCTCT CTCTAAATCA AGCTCAGTGT CCCTGGTGCC TCCAGGTTCT
    TCTCACACAC TTCCTAGAAG AGCACAGTCC CTTAAAGCAC CCTCTATTGC TACAATTAAA
    AGTCTAGCAG ACTATAACTT TAGTTACACA AGTTCTAGAG ATGCCTTTGG CTACGCTACC
    CTGAAAAGAT TACAACAGCA AAGAATGCAT CCATCCTTAT CTCATTCTGA AGCTTTGGCA
    TCCCCAGCAA AAGATGTGCT GTTTACTGAT ACCATCACCA TGAAGGCCAA CAGCTTTGAG
    TCCAGGTTAA CACCAAGCAG GATCAATTTT AAAAAGAAGC ATGTCGGGGG AATCAGGAGC
    TTAAGACCTA CAATAACAAA CAACTTTTTC AGGTTCATGA AAGCTTTAAG TTATGCTTCG
    CTAGATAAAG AAGATTTATT AAGTCCTATT AACCAAAATA CGCTGCAGCG CTCCTCCTCC
    GTGAGGTCCA TGGTGTCCAG TGCGACATAC GGTAGCTCAG ATGACTATAT TGGTCTTGCT
    CTTCCAGTAG ATATCAATGA TATATTCCAG GTAAAGGATA TTCCTTATTT TCAAACAAAA
    AACATACCTC CACATGATGA TCGAGGTGCA AGAGTATTTG CCCATGATGT TGGAGGTCTT
    CCATCTGGAA CTGGAGGTCT TGTAAAAAAC TCTTTTCACT TGCTACGACA GCAGATGAGT
    CTTACGGAAA TAATGAATTC AATCCATTCA GATGCTTCTC TGTTTTTAGA AAGTACAGAA
    GACACTGGAC TACAGGAACA TACAGATGAT AACTGCCTTT ATTGTGTCTG TATTGAAATT
    CTGGGTTTCC AGCCCAGTAA CCAACTGAGT GCAATATGTA GTCATTCAGA CCTTCAAGAC
    ATTCCATATT CTGATTGGTG TGAGCAGACT ATCCATAATC CCTTAGAAGT CGTTCCCTCT
    AAATTTTCTG GCATTTCTGG ATGCAGTGAT GGAGTGTCTC AAGAAGGCTC AGCCAGCAGC
    ACCAAAAGTA CAGAATTGCT ACTAGGTGTT AAAACAATTC CAGATGATAC ACCAATGTGC
    CGTATACTCC TTCGTAAAGA AGTTCTGAGA TTAGTCATTA ATTTGAGTAG TTCAGTTTCA
    ACTAAATGTC ATGAAACTGG GCTTTTAACA ATTAAAGAGA AGTATCCTCA AACATTTGAT
    GACATATGCC TTTACTCTGA GGTTTCCCAT TTGCTGTCAC ACTGCACATT TAGACTTCCA
    TGTCGGAGGT TCATACAAGA ATTATTTCAG GATGTACAGT TTTTACAAAT GCATGAAGAA
    GCAGAGGCTG TCTTGGCAAC ACCACCAAAG CAAACTATAG TCGATACATC TGCTGAATCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.