TOP3A cDNA ORF clone, Bos taurus(cattle)

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa51381 XM_010816034.2
Latest version!
Bos taurus topoisomerase (DNA) III alpha (TOP3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa93871 XM_015458728.2
Latest version!
Bos taurus DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa37350 NM_001077975.2
Latest version!
Bos taurus DNA topoisomerase III alpha (TOP3A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa93871 XM_015458728.1 Bos taurus topoisomerase (DNA) III alpha (TOP3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa51381
    Clone ID Related Accession (Same CDS sequence) XM_010816034.2
    Accession Version XM_010816034.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 3-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTTC CGGCCGCCCA CCGCGCTCTC CGGGGGCTGC CGCGGCCCGG TACTCGGGCC 
    CTGACTCGCG CCGCCATGGA GGTGGCCCTG CGAGGCGTGC GGAAAATCCT TTGCGTGGCC
    GAGAAGAACG ACGCGGCCAA GGGCATAGCC GACTTGCTGT CTGGCGGCCG CATGCGGCGG
    AGGGAAGGAC TTTCAAGATT CAACAAGATC TATGAGTTTG ATTATCATCT GTGTGGCCAG
    AATGTCACCA TGGTAATGAC CTCAGTCTCA GGACACTTGC TGGCCCATGA TTTCCGGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCT CTTGTCCTGT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTCTGG AGCGAGAGGC CCAGCAATGC
    CAGGCCCTGG TGATTTGGAC CGACTGCGAC AGAGAGGGTG AAAACATTGG GTTTGAGGTC
    ATCCACGTGT GCAAGGCCGT GAAGCCCAGC CTGCAGGTGC TGCGAGCCCG TTTCTCCGAG
    ATCACCACCC GCGCTGTCCG GACGGCATGT GAAAACCTTA CGGAGCCCGA CCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGCGGA TCGGGGCGGC CTTCACCAGG
    TTCCAGACGC TGCGGCTGCA GAAGATCTTC CCCGAGGTGC TGGCAGAGCA GCTTATCAGC
    TACGGCAGCT GCCAGTTCCC CACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCATTTGTGC CAGAAGTCTT CCACAAGATC CGAGTGACTC ATGACCACAG GGACGGCGTG
    GTAGAGTTCA GCTGGAAAAG GCATCGTCTC TTCAATCACA CGGCATGTCT CGTCCTCTAC
    CAGATGTGCA TGGAGGATCC CAGGGCGATG GTGGTAGAGG TCAGGTCAAA AGCCAAGAGC
    AAGTGGAGGC CTCAGGCTCT GGACACTGTG GAGCTGGAGA AGCTGGCATC TCGGAAGTTG
    AGGATAAATG CTAAAGAAAC CATGAGGATT GCAGAAAAGC TCTACACCCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC GAACATTTTC CCCAAAGACC TAGACCTGGC GGCACTGGTG
    GAGCAACAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCTC GAAACATTCT AGAGCGCGGT
    GGCCCCACCC CACGCAATGG GAATAAGTCT GACCAAGCGC ACCCTCCCAT CCACCCCACC
    AAATACACTG ACAGCCTGCA GGGGGATGAG CAGCGACTGT ACGAGCTCAT CGTCCGCCAC
    TTCCTGGCCT GCTGCTCGCA GGATGCCCAG GGCCAGGAGA CCACTGTGGA GATCGACATC
    GCGCAGGAGC GCTTCGTGGC CCACGGCCTC ATGATCCTGG CCCGGAACTA TCTGGACGTG
    TACCCGTATG ATCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGCAAGG CACCTGCTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGG GAGACCAGTC CCCCCCAGCT GCTCACCGAG
    GCTGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CGGACGCCAC GCACGCTGAG
    CACATCGAGA CCATCAAGGC CCGGATGTAC GTCGGGCTCA CGCCAGATAA GCGCTTTCTC
    CCTGGGCACC TGGGCATGGG GCTCGTGGAA GGCTATGACT CCATGGGCTA CGAGATGTCC
    AAGCCTGATC TGCGGGCCGA GCTGGAGGCT GACCTGAAGC TCATCTGCGA GGGGAAGAAG
    GACAAGCTGG TGGTCCTGCA TCAGCAGGTG CAGAAGTACA AGCAGGTGTT CATCGAAGCC
    GTGGCCAAAG CCAGGAAGTT GGACGAGGCC TTGTCCCAGT ACTTCGGGGC GGCTACAGAG
    GTAGCCCCAC AGGACGCCCT CCCGGCTGTG CCCGAGCCCA TCAGGAAGTG TCCTCAGTGC
    AGCCGGGACA TGGTTCTTAA GACCAGAAAG AGTGGCGGGT TTTACCTCGG TTGCACGGGC
    TTCCCTGAGT GTCGGTCGGC TGTCTGGTTC CCGGACAGCG TGCTGGAGGC CAGCAGGGAT
    GTGAGCGTGT GCCCAGTGTG CCAGCCGCAC CCAGTTTACA GGTTGAAGTT CAAGTTTAAA
    CGAGGCAGCC TGCCCCCAAC CATGCCCCTA GAATTTGTCG GCTGCATCGG CGGGTGTGAT
    GAGACCCTGA GGGAGATCCT GGCCCTCAGG TTCTCCCAGG GCGCTGCCCG AGTCAGCCAG
    CCTGCCAGCC AGACCTCCGG CTACCTGCAG GCTAGCCAGT CCCTGAACAG AATGGACAGT
    GGCCAGCAGG GCCAGCCCCG GCCCCCCGTC ACCAGGCCCT CGAAGGCTTT GACCAGGACT
    CTTCCTCCAC CCGCCGTTGA GAGTGAGAGC AACTCGGTGA CCTGCAACTG TGGCCAGGAG
    GCCCTGCTGC TCACCGTCCG GAAGGAGGGC CCCAACCAGG GCCGCCAGTT CTACAAGTGT
    GGCAGTGGTG GCTGCAGCTT CTTTCTATGG GCTGACAGCG GCCACCCAGG AGCCGGGCCT
    CCCTCCTCAG CGTCCAGACC CCCAGGCGGC CCCCTGGAAC ACCCCCCGGG CTCAGGGACT
    CTCTTGGGCG GGTCCAGCAG GCCTGGCAGT GGTGGCAGCG GTGGCGCATC CTGCCTGTGT
    GGCCAGCCGG CTGTCACGCG CACCGTGCAG AAGGAGGGGC CCAACCAGGG CCGCCAGTTC
    CACACGTGTG CCCAGCCCCG TGAGCACCAG TGCGGCTTCT TCCAGTGGGT GGACGAGAAC
    GTGGCCCCAG CTGTGATCCT GACACATACG TGGCAGGTCT GCACACACAG GAAGAGCCCA
    GAGCCTGGGT GCAGGCCAGG GGTTGGAGAC GCCACTGCTC CAAGGGACCC ACGTCTGCCT
    TGTAGGAGGC AGTCTGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814336.1
    CDS152..3112
    Translation

    Target ORF information:

    RefSeq Version XM_010816034.2
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) III alpha (TOP3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816034.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATATTTC CGGCCGCCCA CCGCGCTCTC CGGGGGCTGC CGCGGCCCGG TACTCGGGCC 
    CTGACTCGCG CCGCCATGGA GGTGGCCCTG CGAGGCGTGC GGAAAATCCT TTGCGTGGCC
    GAGAAGAACG ACGCGGCCAA GGGCATAGCC GACTTGCTGT CTGGCGGCCG CATGCGGCGG
    AGGGAAGGAC TTTCAAGATT CAACAAGATC TATGAGTTTG ATTATCATCT GTGTGGCCAG
    AATGTCACCA TGGTAATGAC CTCAGTCTCA GGACACTTGC TGGCCCATGA TTTCCGGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCT CTTGTCCTGT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTCTGG AGCGAGAGGC CCAGCAATGC
    CAGGCCCTGG TGATTTGGAC CGACTGCGAC AGAGAGGGTG AAAACATTGG GTTTGAGGTC
    ATCCACGTGT GCAAGGCCGT GAAGCCCAGC CTGCAGGTGC TGCGAGCCCG TTTCTCCGAG
    ATCACCACCC GCGCTGTCCG GACGGCATGT GAAAACCTTA CGGAGCCCGA CCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGCGGA TCGGGGCGGC CTTCACCAGG
    TTCCAGACGC TGCGGCTGCA GAAGATCTTC CCCGAGGTGC TGGCAGAGCA GCTTATCAGC
    TACGGCAGCT GCCAGTTCCC CACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCATTTGTGC CAGAAGTCTT CCACAAGATC CGAGTGACTC ATGACCACAG GGACGGCGTG
    GTAGAGTTCA GCTGGAAAAG GCATCGTCTC TTCAATCACA CGGCATGTCT CGTCCTCTAC
    CAGATGTGCA TGGAGGATCC CAGGGCGATG GTGGTAGAGG TCAGGTCAAA AGCCAAGAGC
    AAGTGGAGGC CTCAGGCTCT GGACACTGTG GAGCTGGAGA AGCTGGCATC TCGGAAGTTG
    AGGATAAATG CTAAAGAAAC CATGAGGATT GCAGAAAAGC TCTACACCCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC GAACATTTTC CCCAAAGACC TAGACCTGGC GGCACTGGTG
    GAGCAACAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCTC GAAACATTCT AGAGCGCGGT
    GGCCCCACCC CACGCAATGG GAATAAGTCT GACCAAGCGC ACCCTCCCAT CCACCCCACC
    AAATACACTG ACAGCCTGCA GGGGGATGAG CAGCGACTGT ACGAGCTCAT CGTCCGCCAC
    TTCCTGGCCT GCTGCTCGCA GGATGCCCAG GGCCAGGAGA CCACTGTGGA GATCGACATC
    GCGCAGGAGC GCTTCGTGGC CCACGGCCTC ATGATCCTGG CCCGGAACTA TCTGGACGTG
    TACCCGTATG ATCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGCAAGG CACCTGCTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGG GAGACCAGTC CCCCCCAGCT GCTCACCGAG
    GCTGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CGGACGCCAC GCACGCTGAG
    CACATCGAGA CCATCAAGGC CCGGATGTAC GTCGGGCTCA CGCCAGATAA GCGCTTTCTC
    CCTGGGCACC TGGGCATGGG GCTCGTGGAA GGCTATGACT CCATGGGCTA CGAGATGTCC
    AAGCCTGATC TGCGGGCCGA GCTGGAGGCT GACCTGAAGC TCATCTGCGA GGGGAAGAAG
    GACAAGCTGG TGGTCCTGCA TCAGCAGGTG CAGAAGTACA AGCAGGTGTT CATCGAAGCC
    GTGGCCAAAG CCAGGAAGTT GGACGAGGCC TTGTCCCAGT ACTTCGGGGC GGCTACAGAG
    GTAGCCCCAC AGGACGCCCT CCCGGCTGTG CCCGAGCCCA TCAGGAAGTG TCCTCAGTGC
    AGCCGGGACA TGGTTCTTAA GACCAGAAAG AGTGGCGGGT TTTACCTCGG TTGCACGGGC
    TTCCCTGAGT GTCGGTCGGC TGTCTGGTTC CCGGACAGCG TGCTGGAGGC CAGCAGGGAT
    GTGAGCGTGT GCCCAGTGTG CCAGCCGCAC CCAGTTTACA GGTTGAAGTT CAAGTTTAAA
    CGAGGCAGCC TGCCCCCAAC CATGCCCCTA GAATTTGTCG GCTGCATCGG CGGGTGTGAT
    GAGACCCTGA GGGAGATCCT GGCCCTCAGG TTCTCCCAGG GCGCTGCCCG AGTCAGCCAG
    CCTGCCAGCC AGACCTCCGG CTACCTGCAG GCTAGCCAGT CCCTGAACAG AATGGACAGT
    GGCCAGCAGG GCCAGCCCCG GCCCCCCGTC ACCAGGCCCT CGAAGGCTTT GACCAGGACT
    CTTCCTCCAC CCGCCGTTGA GAGTGAGAGC AACTCGGTGA CCTGCAACTG TGGCCAGGAG
    GCCCTGCTGC TCACCGTCCG GAAGGAGGGC CCCAACCAGG GCCGCCAGTT CTACAAGTGT
    GGCAGTGGTG GCTGCAGCTT CTTTCTATGG GCTGACAGCG GCCACCCAGG AGCCGGGCCT
    CCCTCCTCAG CGTCCAGACC CCCAGGCGGC CCCCTGGAAC ACCCCCCGGG CTCAGGGACT
    CTCTTGGGCG GGTCCAGCAG GCCTGGCAGT GGTGGCAGCG GTGGCGCATC CTGCCTGTGT
    GGCCAGCCGG CTGTCACGCG CACCGTGCAG AAGGAGGGGC CCAACCAGGG CCGCCAGTTC
    CACACGTGTG CCCAGCCCCG TGAGCACCAG TGCGGCTTCT TCCAGTGGGT GGACGAGAAC
    GTGGCCCCAG CTGTGATCCT GACACATACG TGGCAGGTCT GCACACACAG GAAGAGCCCA
    GAGCCTGGGT GCAGGCCAGG GGTTGGAGAC GCCACTGCTC CAAGGGACCC ACGTCTGCCT
    TGTAGGAGGC AGTCTGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93871
    Clone ID Related Accession (Same CDS sequence) XM_015458728.2 , XM_015458728.1
    Accession Version XM_015458728.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 3-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTTGA TTATCATCTG TGTGGCCAGG CAGAGCTGCA ATCCTCTTGT CCTGTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TCTGGAGCGA
    GAGGCCCAGC AATGCCAGGC CCTGGTGATT TGGACCGACT GCGACAGAGA GGGTGAAAAC
    ATTGGGTTTG AGGTCATCCA CGTGTGCAAG GCCGTGAAGC CCAGCCTGCA GGTGCTGCGA
    GCCCGTTTCT CCGAGATCAC CACCCGCGCT GTCCGGACGG CATGTGAAAA CCTTACGGAG
    CCCGACCAGA GAGTGAGCGA TGCCGTGGAT GTGAGGCAGG AGTTGGACCT GCGGATCGGG
    GCGGCCTTCA CCAGGTTCCA GACGCTGCGG CTGCAGAAGA TCTTCCCCGA GGTGCTGGCA
    GAGCAGCTTA TCAGCTACGG CAGCTGCCAG TTCCCCACCC TGGGGTTCGT GGTGGAGAGG
    TTCAAAGCCA TTCAGGCATT TGTGCCAGAA GTCTTCCACA AGATCCGAGT GACTCATGAC
    CACAGGGACG GCGTGGTAGA GTTCAGCTGG AAAAGGCATC GTCTCTTCAA TCACACGGCA
    TGTCTCGTCC TCTACCAGAT GTGCATGGAG GATCCCAGGG CGATGGTGGT AGAGGTCAGG
    TCAAAAGCCA AGAGCAAGTG GAGGCCTCAG GCTCTGGACA CTGTGGAGCT GGAGAAGCTG
    GCATCTCGGA AGTTGAGGAT AAATGCTAAA GAAACCATGA GGATTGCAGA AAAGCTCTAC
    ACCCAAGGGT ACATCAGCTA TCCTCGAACT GAAACGAACA TTTTCCCCAA AGACCTAGAC
    CTGGCGGCAC TGGTGGAGCA ACAGACCCCG GATCCACGCT GGGGGGCCTT TGCTCGAAAC
    ATTCTAGAGC GCGGTGGCCC CACCCCACGC AATGGGAATA AGTCTGACCA AGCGCACCCT
    CCCATCCACC CCACCAAATA CACTGACAGC CTGCAGGGGG ATGAGCAGCG ACTGTACGAG
    CTCATCGTCC GCCACTTCCT GGCCTGCTGC TCGCAGGATG CCCAGGGCCA GGAGACCACT
    GTGGAGATCG ACATCGCGCA GGAGCGCTTC GTGGCCCACG GCCTCATGAT CCTGGCCCGG
    AACTATCTGG ACGTGTACCC GTATGATCGC TGGAGTGACA AGACCCTCCC TGTCTATGAG
    CAAGGCACCT GCTTTCAGCC CAGCACTGTG GAGATGGTGG ACGGGGAGAC CAGTCCCCCC
    CAGCTGCTCA CCGAGGCTGA CCTCATTGCC CTCATGGAGA AGCACGGCAT TGGTACGGAC
    GCCACGCACG CTGAGCACAT CGAGACCATC AAGGCCCGGA TGTACGTCGG GCTCACGCCA
    GATAAGCGCT TTCTCCCTGG GCACCTGGGC ATGGGGCTCG TGGAAGGCTA TGACTCCATG
    GGCTACGAGA TGTCCAAGCC TGATCTGCGG GCCGAGCTGG AGGCTGACCT GAAGCTCATC
    TGCGAGGGGA AGAAGGACAA GCTGGTGGTC CTGCATCAGC AGGTGCAGAA GTACAAGCAG
    GTGTTCATCG AAGCCGTGGC CAAAGCCAGG AAGTTGGACG AGGCCTTGTC CCAGTACTTC
    GGGGCGGCTA CAGAGGTAGC CCCACAGGAC GCCCTCCCGG CTGTGCCCGA GCCCATCAGG
    AAGTGTCCTC AGTGCAGCCG GGACATGGTT CTTAAGACCA GAAAGAGTGG CGGGTTTTAC
    CTCGGTTGCA CGGGCTTCCC TGAGTGTCGG TCGGCTGTCT GGTTCCCGGA CAGCGTGCTG
    GAGGCCAGCA GGGATGTGAG CGTGTGCCCA GTGTGCCAGC CGCACCCAGT TTACAGGTTG
    AAGTTCAAGT TTAAACGAGG CAGCCTGCCC CCAACCATGC CCCTAGAATT TGTCGGCTGC
    ATCGGCGGGT GTGATGAGAC CCTGAGGGAG ATCCTGGCCC TCAGGTTCTC CCAGGGCGCT
    GCCCGAGTCA GCCAGCCTGC CAGCCAGACC TCCGGCTACC TGCAGGCTAG CCAGTCCCTG
    AACAGAATGG ACAGTGGCCA GCAGGGCCAG CCCCGGCCCC CCGTCACCAG GCCCTCGAAG
    GCTTTGACCA GGACTCTTCC TCCACCCGCC GTTGAGAGTG AGAGCAACTC GGTGACCTGC
    AACTGTGGCC AGGAGGCCCT GCTGCTCACC GTCCGGAAGG AGGGCCCCAA CCAGGGCCGC
    CAGTTCTACA AGTGTGGCAG TGGTGGCTGC AGCTTCTTTC TATGGGCTGA CAGCGGCCAC
    CCAGGAGCCG GGCCTCCCTC CTCAGCGTCC AGACCCCCAG GCGGCCCCCT GGAACACCCC
    CCGGGCTCAG GGACTCTCTT GGGCGGGTCC AGCAGGCCTG GCAGTGGTGG CAGCGGTGGC
    GCATCCTGCC TGTGTGGCCA GCCGGCTGTC ACGCGCACCG TGCAGAAGGA GGGGCCCAAC
    CAGGGCCGCC AGTTCCACAC GTGTGCCCAG CCCCGTGAGC ACCAGTGCGG CTTCTTCCAG
    TGGGTGGACG AGAACGTGGC CCCAGGGACC TTCGGAGGCC CCCTCTGGCC AGGAAGCAGG
    AGAGGAACCC AGGGGCTGGA GGCCAGAAGC AAGAGGCCTC GGGCCGGCTC CTCAGACACA
    GGGTCTGCAG CCAAGAAACC CCGGAAGTGC AGCCTTTGCC ACCAGCCTGG ACATACCCGT
    CCCTTCTGTC CTCAGAACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314214.1
    CDS172..2895
    Translation

    Target ORF information:

    RefSeq Version XM_015458728.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTTGA TTATCATCTG TGTGGCCAGG CAGAGCTGCA ATCCTCTTGT CCTGTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TCTGGAGCGA
    GAGGCCCAGC AATGCCAGGC CCTGGTGATT TGGACCGACT GCGACAGAGA GGGTGAAAAC
    ATTGGGTTTG AGGTCATCCA CGTGTGCAAG GCCGTGAAGC CCAGCCTGCA GGTGCTGCGA
    GCCCGTTTCT CCGAGATCAC CACCCGCGCT GTCCGGACGG CATGTGAAAA CCTTACGGAG
    CCCGACCAGA GAGTGAGCGA TGCCGTGGAT GTGAGGCAGG AGTTGGACCT GCGGATCGGG
    GCGGCCTTCA CCAGGTTCCA GACGCTGCGG CTGCAGAAGA TCTTCCCCGA GGTGCTGGCA
    GAGCAGCTTA TCAGCTACGG CAGCTGCCAG TTCCCCACCC TGGGGTTCGT GGTGGAGAGG
    TTCAAAGCCA TTCAGGCATT TGTGCCAGAA GTCTTCCACA AGATCCGAGT GACTCATGAC
    CACAGGGACG GCGTGGTAGA GTTCAGCTGG AAAAGGCATC GTCTCTTCAA TCACACGGCA
    TGTCTCGTCC TCTACCAGAT GTGCATGGAG GATCCCAGGG CGATGGTGGT AGAGGTCAGG
    TCAAAAGCCA AGAGCAAGTG GAGGCCTCAG GCTCTGGACA CTGTGGAGCT GGAGAAGCTG
    GCATCTCGGA AGTTGAGGAT AAATGCTAAA GAAACCATGA GGATTGCAGA AAAGCTCTAC
    ACCCAAGGGT ACATCAGCTA TCCTCGAACT GAAACGAACA TTTTCCCCAA AGACCTAGAC
    CTGGCGGCAC TGGTGGAGCA ACAGACCCCG GATCCACGCT GGGGGGCCTT TGCTCGAAAC
    ATTCTAGAGC GCGGTGGCCC CACCCCACGC AATGGGAATA AGTCTGACCA AGCGCACCCT
    CCCATCCACC CCACCAAATA CACTGACAGC CTGCAGGGGG ATGAGCAGCG ACTGTACGAG
    CTCATCGTCC GCCACTTCCT GGCCTGCTGC TCGCAGGATG CCCAGGGCCA GGAGACCACT
    GTGGAGATCG ACATCGCGCA GGAGCGCTTC GTGGCCCACG GCCTCATGAT CCTGGCCCGG
    AACTATCTGG ACGTGTACCC GTATGATCGC TGGAGTGACA AGACCCTCCC TGTCTATGAG
    CAAGGCACCT GCTTTCAGCC CAGCACTGTG GAGATGGTGG ACGGGGAGAC CAGTCCCCCC
    CAGCTGCTCA CCGAGGCTGA CCTCATTGCC CTCATGGAGA AGCACGGCAT TGGTACGGAC
    GCCACGCACG CTGAGCACAT CGAGACCATC AAGGCCCGGA TGTACGTCGG GCTCACGCCA
    GATAAGCGCT TTCTCCCTGG GCACCTGGGC ATGGGGCTCG TGGAAGGCTA TGACTCCATG
    GGCTACGAGA TGTCCAAGCC TGATCTGCGG GCCGAGCTGG AGGCTGACCT GAAGCTCATC
    TGCGAGGGGA AGAAGGACAA GCTGGTGGTC CTGCATCAGC AGGTGCAGAA GTACAAGCAG
    GTGTTCATCG AAGCCGTGGC CAAAGCCAGG AAGTTGGACG AGGCCTTGTC CCAGTACTTC
    GGGGCGGCTA CAGAGGTAGC CCCACAGGAC GCCCTCCCGG CTGTGCCCGA GCCCATCAGG
    AAGTGTCCTC AGTGCAGCCG GGACATGGTT CTTAAGACCA GAAAGAGTGG CGGGTTTTAC
    CTCGGTTGCA CGGGCTTCCC TGAGTGTCGG TCGGCTGTCT GGTTCCCGGA CAGCGTGCTG
    GAGGCCAGCA GGGATGTGAG CGTGTGCCCA GTGTGCCAGC CGCACCCAGT TTACAGGTTG
    AAGTTCAAGT TTAAACGAGG CAGCCTGCCC CCAACCATGC CCCTAGAATT TGTCGGCTGC
    ATCGGCGGGT GTGATGAGAC CCTGAGGGAG ATCCTGGCCC TCAGGTTCTC CCAGGGCGCT
    GCCCGAGTCA GCCAGCCTGC CAGCCAGACC TCCGGCTACC TGCAGGCTAG CCAGTCCCTG
    AACAGAATGG ACAGTGGCCA GCAGGGCCAG CCCCGGCCCC CCGTCACCAG GCCCTCGAAG
    GCTTTGACCA GGACTCTTCC TCCACCCGCC GTTGAGAGTG AGAGCAACTC GGTGACCTGC
    AACTGTGGCC AGGAGGCCCT GCTGCTCACC GTCCGGAAGG AGGGCCCCAA CCAGGGCCGC
    CAGTTCTACA AGTGTGGCAG TGGTGGCTGC AGCTTCTTTC TATGGGCTGA CAGCGGCCAC
    CCAGGAGCCG GGCCTCCCTC CTCAGCGTCC AGACCCCCAG GCGGCCCCCT GGAACACCCC
    CCGGGCTCAG GGACTCTCTT GGGCGGGTCC AGCAGGCCTG GCAGTGGTGG CAGCGGTGGC
    GCATCCTGCC TGTGTGGCCA GCCGGCTGTC ACGCGCACCG TGCAGAAGGA GGGGCCCAAC
    CAGGGCCGCC AGTTCCACAC GTGTGCCCAG CCCCGTGAGC ACCAGTGCGG CTTCTTCCAG
    TGGGTGGACG AGAACGTGGC CCCAGGGACC TTCGGAGGCC CCCTCTGGCC AGGAAGCAGG
    AGAGGAACCC AGGGGCTGGA GGCCAGAAGC AAGAGGCCTC GGGCCGGCTC CTCAGACACA
    GGGTCTGCAG CCAAGAAACC CCGGAAGTGC AGCCTTTGCC ACCAGCCTGG ACATACCCGT
    CCCTTCTGTC CTCAGAACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93871
    Clone ID Related Accession (Same CDS sequence) XM_015458728.2 , XM_015458728.1
    Accession Version XM_015458728.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 3-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTTGA TTATCATCTG TGTGGCCAGG CAGAGCTGCA ATCCTCTTGT CCTGTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TCTGGAGCGA
    GAGGCCCAGC AATGCCAGGC CCTGGTGATT TGGACCGACT GCGACAGAGA GGGTGAAAAC
    ATTGGGTTTG AGGTCATCCA CGTGTGCAAG GCCGTGAAGC CCAGCCTGCA GGTGCTGCGA
    GCCCGTTTCT CCGAGATCAC CACCCGCGCT GTCCGGACGG CATGTGAAAA CCTTACGGAG
    CCCGACCAGA GAGTGAGCGA TGCCGTGGAT GTGAGGCAGG AGTTGGACCT GCGGATCGGG
    GCGGCCTTCA CCAGGTTCCA GACGCTGCGG CTGCAGAAGA TCTTCCCCGA GGTGCTGGCA
    GAGCAGCTTA TCAGCTACGG CAGCTGCCAG TTCCCCACCC TGGGGTTCGT GGTGGAGAGG
    TTCAAAGCCA TTCAGGCATT TGTGCCAGAA GTCTTCCACA AGATCCGAGT GACTCATGAC
    CACAGGGACG GCGTGGTAGA GTTCAGCTGG AAAAGGCATC GTCTCTTCAA TCACACGGCA
    TGTCTCGTCC TCTACCAGAT GTGCATGGAG GATCCCAGGG CGATGGTGGT AGAGGTCAGG
    TCAAAAGCCA AGAGCAAGTG GAGGCCTCAG GCTCTGGACA CTGTGGAGCT GGAGAAGCTG
    GCATCTCGGA AGTTGAGGAT AAATGCTAAA GAAACCATGA GGATTGCAGA AAAGCTCTAC
    ACCCAAGGGT ACATCAGCTA TCCTCGAACT GAAACGAACA TTTTCCCCAA AGACCTAGAC
    CTGGCGGCAC TGGTGGAGCA ACAGACCCCG GATCCACGCT GGGGGGCCTT TGCTCGAAAC
    ATTCTAGAGC GCGGTGGCCC CACCCCACGC AATGGGAATA AGTCTGACCA AGCGCACCCT
    CCCATCCACC CCACCAAATA CACTGACAGC CTGCAGGGGG ATGAGCAGCG ACTGTACGAG
    CTCATCGTCC GCCACTTCCT GGCCTGCTGC TCGCAGGATG CCCAGGGCCA GGAGACCACT
    GTGGAGATCG ACATCGCGCA GGAGCGCTTC GTGGCCCACG GCCTCATGAT CCTGGCCCGG
    AACTATCTGG ACGTGTACCC GTATGATCGC TGGAGTGACA AGACCCTCCC TGTCTATGAG
    CAAGGCACCT GCTTTCAGCC CAGCACTGTG GAGATGGTGG ACGGGGAGAC CAGTCCCCCC
    CAGCTGCTCA CCGAGGCTGA CCTCATTGCC CTCATGGAGA AGCACGGCAT TGGTACGGAC
    GCCACGCACG CTGAGCACAT CGAGACCATC AAGGCCCGGA TGTACGTCGG GCTCACGCCA
    GATAAGCGCT TTCTCCCTGG GCACCTGGGC ATGGGGCTCG TGGAAGGCTA TGACTCCATG
    GGCTACGAGA TGTCCAAGCC TGATCTGCGG GCCGAGCTGG AGGCTGACCT GAAGCTCATC
    TGCGAGGGGA AGAAGGACAA GCTGGTGGTC CTGCATCAGC AGGTGCAGAA GTACAAGCAG
    GTGTTCATCG AAGCCGTGGC CAAAGCCAGG AAGTTGGACG AGGCCTTGTC CCAGTACTTC
    GGGGCGGCTA CAGAGGTAGC CCCACAGGAC GCCCTCCCGG CTGTGCCCGA GCCCATCAGG
    AAGTGTCCTC AGTGCAGCCG GGACATGGTT CTTAAGACCA GAAAGAGTGG CGGGTTTTAC
    CTCGGTTGCA CGGGCTTCCC TGAGTGTCGG TCGGCTGTCT GGTTCCCGGA CAGCGTGCTG
    GAGGCCAGCA GGGATGTGAG CGTGTGCCCA GTGTGCCAGC CGCACCCAGT TTACAGGTTG
    AAGTTCAAGT TTAAACGAGG CAGCCTGCCC CCAACCATGC CCCTAGAATT TGTCGGCTGC
    ATCGGCGGGT GTGATGAGAC CCTGAGGGAG ATCCTGGCCC TCAGGTTCTC CCAGGGCGCT
    GCCCGAGTCA GCCAGCCTGC CAGCCAGACC TCCGGCTACC TGCAGGCTAG CCAGTCCCTG
    AACAGAATGG ACAGTGGCCA GCAGGGCCAG CCCCGGCCCC CCGTCACCAG GCCCTCGAAG
    GCTTTGACCA GGACTCTTCC TCCACCCGCC GTTGAGAGTG AGAGCAACTC GGTGACCTGC
    AACTGTGGCC AGGAGGCCCT GCTGCTCACC GTCCGGAAGG AGGGCCCCAA CCAGGGCCGC
    CAGTTCTACA AGTGTGGCAG TGGTGGCTGC AGCTTCTTTC TATGGGCTGA CAGCGGCCAC
    CCAGGAGCCG GGCCTCCCTC CTCAGCGTCC AGACCCCCAG GCGGCCCCCT GGAACACCCC
    CCGGGCTCAG GGACTCTCTT GGGCGGGTCC AGCAGGCCTG GCAGTGGTGG CAGCGGTGGC
    GCATCCTGCC TGTGTGGCCA GCCGGCTGTC ACGCGCACCG TGCAGAAGGA GGGGCCCAAC
    CAGGGCCGCC AGTTCCACAC GTGTGCCCAG CCCCGTGAGC ACCAGTGCGG CTTCTTCCAG
    TGGGTGGACG AGAACGTGGC CCCAGGGACC TTCGGAGGCC CCCTCTGGCC AGGAAGCAGG
    AGAGGAACCC AGGGGCTGGA GGCCAGAAGC AAGAGGCCTC GGGCCGGCTC CTCAGACACA
    GGGTCTGCAG CCAAGAAACC CCGGAAGTGC AGCCTTTGCC ACCAGCCTGG ACATACCCGT
    CCCTTCTGTC CTCAGAACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314214.1
    CDS136..2859
    Translation

    Target ORF information:

    RefSeq Version XM_015458728.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) III alpha (TOP3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTTGA TTATCATCTG TGTGGCCAGG CAGAGCTGCA ATCCTCTTGT CCTGTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TCTGGAGCGA
    GAGGCCCAGC AATGCCAGGC CCTGGTGATT TGGACCGACT GCGACAGAGA GGGTGAAAAC
    ATTGGGTTTG AGGTCATCCA CGTGTGCAAG GCCGTGAAGC CCAGCCTGCA GGTGCTGCGA
    GCCCGTTTCT CCGAGATCAC CACCCGCGCT GTCCGGACGG CATGTGAAAA CCTTACGGAG
    CCCGACCAGA GAGTGAGCGA TGCCGTGGAT GTGAGGCAGG AGTTGGACCT GCGGATCGGG
    GCGGCCTTCA CCAGGTTCCA GACGCTGCGG CTGCAGAAGA TCTTCCCCGA GGTGCTGGCA
    GAGCAGCTTA TCAGCTACGG CAGCTGCCAG TTCCCCACCC TGGGGTTCGT GGTGGAGAGG
    TTCAAAGCCA TTCAGGCATT TGTGCCAGAA GTCTTCCACA AGATCCGAGT GACTCATGAC
    CACAGGGACG GCGTGGTAGA GTTCAGCTGG AAAAGGCATC GTCTCTTCAA TCACACGGCA
    TGTCTCGTCC TCTACCAGAT GTGCATGGAG GATCCCAGGG CGATGGTGGT AGAGGTCAGG
    TCAAAAGCCA AGAGCAAGTG GAGGCCTCAG GCTCTGGACA CTGTGGAGCT GGAGAAGCTG
    GCATCTCGGA AGTTGAGGAT AAATGCTAAA GAAACCATGA GGATTGCAGA AAAGCTCTAC
    ACCCAAGGGT ACATCAGCTA TCCTCGAACT GAAACGAACA TTTTCCCCAA AGACCTAGAC
    CTGGCGGCAC TGGTGGAGCA ACAGACCCCG GATCCACGCT GGGGGGCCTT TGCTCGAAAC
    ATTCTAGAGC GCGGTGGCCC CACCCCACGC AATGGGAATA AGTCTGACCA AGCGCACCCT
    CCCATCCACC CCACCAAATA CACTGACAGC CTGCAGGGGG ATGAGCAGCG ACTGTACGAG
    CTCATCGTCC GCCACTTCCT GGCCTGCTGC TCGCAGGATG CCCAGGGCCA GGAGACCACT
    GTGGAGATCG ACATCGCGCA GGAGCGCTTC GTGGCCCACG GCCTCATGAT CCTGGCCCGG
    AACTATCTGG ACGTGTACCC GTATGATCGC TGGAGTGACA AGACCCTCCC TGTCTATGAG
    CAAGGCACCT GCTTTCAGCC CAGCACTGTG GAGATGGTGG ACGGGGAGAC CAGTCCCCCC
    CAGCTGCTCA CCGAGGCTGA CCTCATTGCC CTCATGGAGA AGCACGGCAT TGGTACGGAC
    GCCACGCACG CTGAGCACAT CGAGACCATC AAGGCCCGGA TGTACGTCGG GCTCACGCCA
    GATAAGCGCT TTCTCCCTGG GCACCTGGGC ATGGGGCTCG TGGAAGGCTA TGACTCCATG
    GGCTACGAGA TGTCCAAGCC TGATCTGCGG GCCGAGCTGG AGGCTGACCT GAAGCTCATC
    TGCGAGGGGA AGAAGGACAA GCTGGTGGTC CTGCATCAGC AGGTGCAGAA GTACAAGCAG
    GTGTTCATCG AAGCCGTGGC CAAAGCCAGG AAGTTGGACG AGGCCTTGTC CCAGTACTTC
    GGGGCGGCTA CAGAGGTAGC CCCACAGGAC GCCCTCCCGG CTGTGCCCGA GCCCATCAGG
    AAGTGTCCTC AGTGCAGCCG GGACATGGTT CTTAAGACCA GAAAGAGTGG CGGGTTTTAC
    CTCGGTTGCA CGGGCTTCCC TGAGTGTCGG TCGGCTGTCT GGTTCCCGGA CAGCGTGCTG
    GAGGCCAGCA GGGATGTGAG CGTGTGCCCA GTGTGCCAGC CGCACCCAGT TTACAGGTTG
    AAGTTCAAGT TTAAACGAGG CAGCCTGCCC CCAACCATGC CCCTAGAATT TGTCGGCTGC
    ATCGGCGGGT GTGATGAGAC CCTGAGGGAG ATCCTGGCCC TCAGGTTCTC CCAGGGCGCT
    GCCCGAGTCA GCCAGCCTGC CAGCCAGACC TCCGGCTACC TGCAGGCTAG CCAGTCCCTG
    AACAGAATGG ACAGTGGCCA GCAGGGCCAG CCCCGGCCCC CCGTCACCAG GCCCTCGAAG
    GCTTTGACCA GGACTCTTCC TCCACCCGCC GTTGAGAGTG AGAGCAACTC GGTGACCTGC
    AACTGTGGCC AGGAGGCCCT GCTGCTCACC GTCCGGAAGG AGGGCCCCAA CCAGGGCCGC
    CAGTTCTACA AGTGTGGCAG TGGTGGCTGC AGCTTCTTTC TATGGGCTGA CAGCGGCCAC
    CCAGGAGCCG GGCCTCCCTC CTCAGCGTCC AGACCCCCAG GCGGCCCCCT GGAACACCCC
    CCGGGCTCAG GGACTCTCTT GGGCGGGTCC AGCAGGCCTG GCAGTGGTGG CAGCGGTGGC
    GCATCCTGCC TGTGTGGCCA GCCGGCTGTC ACGCGCACCG TGCAGAAGGA GGGGCCCAAC
    CAGGGCCGCC AGTTCCACAC GTGTGCCCAG CCCCGTGAGC ACCAGTGCGG CTTCTTCCAG
    TGGGTGGACG AGAACGTGGC CCCAGGGACC TTCGGAGGCC CCCTCTGGCC AGGAAGCAGG
    AGAGGAACCC AGGGGCTGGA GGCCAGAAGC AAGAGGCCTC GGGCCGGCTC CTCAGACACA
    GGGTCTGCAG CCAAGAAACC CCGGAAGTGC AGCCTTTGCC ACCAGCCTGG ACATACCCGT
    CCCTTCTGTC CTCAGAACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37350
    Clone ID Related Accession (Same CDS sequence) NM_001077975.2
    Accession Version NM_001077975.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-13
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 3-alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126547.1. On Aug 30, 2012 this sequence version replaced NM_001077975.1. ##Evidence-Data-START## Transcript exon combination :: BC126547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATATTTC CGGCCGCCCA CCGCGCTCTC CGGGGGCTGC CGCGGCCCGG TACTCGGGCC 
    CTGACTCGCG CCGCCATGGA GGTGGCCCTG CGAGGCGTGC GGAAAATCCT TTGCGTGGCC
    GAGAAGAACG ACGCGGCCAA GGGCATAGCC GACTTGCTGT CTGGCGGCCG CATGCGGCGG
    AGGGAAGGAC TTTCAAGATT CAACAAGATC TATGAGTTTG ATTATCATCT GTGTGGCCAG
    AATGTCACCA TGGTAATGAC CTCAGTCTCA GGACACTTGC TGGCCCATGA TTTCCGGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCT CTTGTCCTGT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTCTGG AGCGAGAGGC CCAGCAATGC
    CAGGCCCTGG TGATTTGGAC CGACTGCGAC AGAGAGGGTG AAAACATTGG GTTTGAGGTC
    ATCCACGTGT GCAAGGCCGT GAAGCCCAGC CTGCAGGTGC TGCGAGCCCG TTTCTCCGAG
    ATCACCACCC GCGCTGTCCG GACGGCATGT GAAAACCTTA CGGAGCCCGA CCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGCGGA TCGGGGCGGC CTTCACCAGG
    TTCCAGACGC TGCGGCTGCA GAAGATCTTC CCCGAGGTGC TGGCAGAGCA GCTTATCAGC
    TACGGCAGCT GCCAGTTCCC CACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCATTTGTGC CAGAAGTCTT CCACAAGATC CGAGTGACTC ATGACCACAG GGACGGCGTG
    GTAGAGTTCA GCTGGAAAAG GCATCGTCTC TTCAATCACA CGGCATGTCT CGTCCTCTAC
    CAGATGTGCA TGGAGGATCC CAGGGCGATG GTGGTAGAGG TCAGGTCAAA AGCCAAGAGC
    AAGTGGAGGC CTCAGGCTCT GGACACTGTG GAGCTGGAGA AGCTGGCATC TCGGAAGTTG
    AGGATAAATG CTAAAGAAAC CATGAGGATT GCAGAAAAGC TCTACACCCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC GAACATTTTC CCCAAAGACC TAGACCTGGC GGCACTGGTG
    GAGCAACAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCTC GAAACATTCT AGAGCGCGGT
    GGCCCCACCC CACGCAATGG GAATAAGTCT GACCAAGCGC ACCCTCCCAT CCACCCCACC
    AAATACACTG ACAGCCTGCA GGGGGATGAG CAGCGACTGT ACGAGCTCAT CGTCCGCCAC
    TTCCTGGCCT GCTGCTCGCA GGATGCCCAG GGCCAGGAGA CCACTGTGGA GATCGACATC
    GCGCAGGAGC GCTTCGTGGC CCACGGCCTC ATGATCCTGG CCCGGAACTA TCTGGACGTG
    TACCCGTATG ATCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGCAAGG CACCTGCTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGG GAGACCAGTC CCCCCCAGCT GCTCACCGAG
    GCTGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CGGACGCCAC GCACGCTGAG
    CACATCGAGA CCATCAAGGC CCGGATGTAC GTCGGGCTCA CGCCAGATAA GCGCTTTCTC
    CCTGGGCACC TGGGCATGGG GCTCGTGGAA GGCTATGACT CCATGGGCTA CGAGATGTCC
    AAGCCTGATC TGCGGGCCGA GCTGGAGGCT GACCTGAAGC TCATCTGCGA GGGGAAGAAG
    GACAAGCTGG TGGTCCTGCA TCAGCAGGTG CAGAAGTACA AGCAGGTGTT CATCGAAGCC
    GTGGCCAAAG CCAGGAAGTT GGACGAGGCC TTGTCCCAGT ACTTCGGGGC GGCTACAGAG
    GTAGCCCCAC AGGACGCCCT CCCGGCTGTG CCCGAGCCCA TCAGGAAGTG TCCTCAGTGC
    AGCCGGGACA TGGTTCTTAA GACCAGAAAG AGTGGCGGGT TTTACCTCGG TTGCACGGGC
    TTCCCTGAGT GTCGGTCGGC TGTCTGGTTC CCGGACAGCG TGCTGGAGGC CAGCAGGGAT
    GTGAGCGTGT GCCCAGTGTG CCAGCCGCAC CCAGTTTACA GGTTGAAGTT CAAGTTTAAA
    CGAGGCAGCC TGCCCCCAAC CATGCCCCTA GAATTTGTCG GCTGCATCGG CGGGTGTGAT
    GAGACCCTGA GGGAGATCCT GGCCCTCAGG TTCTCCCAGG GCGCTGCCCG AGTCAGCCAG
    CCTGCCAGCC AGACCTCCGG CTACCTGCAG GCTAGCCAGT CCCTGAACAG AATGGACAGT
    GGCCAGCAGG GCCAGCCCCG GCCCCCCGTC ACCAGGCCCT CGAAGGCTTT GACCAGGACT
    CTTCCTCCAC CCGCCGTTGA GAGTGAGAGC AACTCGGTGA CCTGCAACTG TGGCCAGGAG
    GCCCTGCTGC TCACCGTCCG GAAGGAGGGC CCCAACCAGG GCCGCCAGTT CTACAAGTGT
    GGCAGTGGTG GCTGCAGCTT CTTTCTATGG GCTGACAGCG GCCACCCAGG AGCCGGGCCT
    CCCTCCTCAG CGTCCAGACC CCCAGGCGGC CCCCTGGAAC ACCCCCCGGG CTCAGGGACT
    CTCTTGGGCG GGTCCAGCAG GCCTGGCAGT GGTGGCAGCG GTGGCGCATC CTGCCTGTGT
    GGCCAGCCGG CTGTCACGCG CACCGTGCAG AAGGAGGGGC CCAACCAGGG CCGCCAGTTC
    CACACGTGTG CCCAGCCCCG TGAGCACCAG TGCGGCTTCT TCCAGTGGGT GGACGAGAAC
    GTGGCCCCAG GGACCTTCGG AGGCCCCCTC TGGCCAGGAA GCAGGAGAGG AACCCAGGGG
    CTGGAGGCCA GAAGCAAGAG GCCTCGGGCC GGCTCCTCAG ACACAGGGTC TGCAACCAAG
    AAACCCCGGA AGTGCAGCCT TTGCCACCAG CCTGGACATA CCCGTCCCTT CTGTCCTCAG
    AACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071443.1
    CDS60..3068
    Translation

    Target ORF information:

    RefSeq Version NM_001077975.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase III alpha (TOP3A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077975.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATATTTC CGGCCGCCCA CCGCGCTCTC CGGGGGCTGC CGCGGCCCGG TACTCGGGCC 
    CTGACTCGCG CCGCCATGGA GGTGGCCCTG CGAGGCGTGC GGAAAATCCT TTGCGTGGCC
    GAGAAGAACG ACGCGGCCAA GGGCATAGCC GACTTGCTGT CTGGCGGCCG CATGCGGCGG
    AGGGAAGGAC TTTCAAGATT CAACAAGATC TATGAGTTTG ATTATCATCT GTGTGGCCAG
    AATGTCACCA TGGTAATGAC CTCAGTCTCA GGACACTTGC TGGCCCATGA TTTCCGGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCT CTTGTCCTGT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTCTGG AGCGAGAGGC CCAGCAATGC
    CAGGCCCTGG TGATTTGGAC CGACTGCGAC AGAGAGGGTG AAAACATTGG GTTTGAGGTC
    ATCCACGTGT GCAAGGCCGT GAAGCCCAGC CTGCAGGTGC TGCGAGCCCG TTTCTCCGAG
    ATCACCACCC GCGCTGTCCG GACGGCATGT GAAAACCTTA CGGAGCCCGA CCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGCGGA TCGGGGCGGC CTTCACCAGG
    TTCCAGACGC TGCGGCTGCA GAAGATCTTC CCCGAGGTGC TGGCAGAGCA GCTTATCAGC
    TACGGCAGCT GCCAGTTCCC CACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCATTTGTGC CAGAAGTCTT CCACAAGATC CGAGTGACTC ATGACCACAG GGACGGCGTG
    GTAGAGTTCA GCTGGAAAAG GCATCGTCTC TTCAATCACA CGGCATGTCT CGTCCTCTAC
    CAGATGTGCA TGGAGGATCC CAGGGCGATG GTGGTAGAGG TCAGGTCAAA AGCCAAGAGC
    AAGTGGAGGC CTCAGGCTCT GGACACTGTG GAGCTGGAGA AGCTGGCATC TCGGAAGTTG
    AGGATAAATG CTAAAGAAAC CATGAGGATT GCAGAAAAGC TCTACACCCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC GAACATTTTC CCCAAAGACC TAGACCTGGC GGCACTGGTG
    GAGCAACAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCTC GAAACATTCT AGAGCGCGGT
    GGCCCCACCC CACGCAATGG GAATAAGTCT GACCAAGCGC ACCCTCCCAT CCACCCCACC
    AAATACACTG ACAGCCTGCA GGGGGATGAG CAGCGACTGT ACGAGCTCAT CGTCCGCCAC
    TTCCTGGCCT GCTGCTCGCA GGATGCCCAG GGCCAGGAGA CCACTGTGGA GATCGACATC
    GCGCAGGAGC GCTTCGTGGC CCACGGCCTC ATGATCCTGG CCCGGAACTA TCTGGACGTG
    TACCCGTATG ATCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGCAAGG CACCTGCTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGG GAGACCAGTC CCCCCCAGCT GCTCACCGAG
    GCTGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CGGACGCCAC GCACGCTGAG
    CACATCGAGA CCATCAAGGC CCGGATGTAC GTCGGGCTCA CGCCAGATAA GCGCTTTCTC
    CCTGGGCACC TGGGCATGGG GCTCGTGGAA GGCTATGACT CCATGGGCTA CGAGATGTCC
    AAGCCTGATC TGCGGGCCGA GCTGGAGGCT GACCTGAAGC TCATCTGCGA GGGGAAGAAG
    GACAAGCTGG TGGTCCTGCA TCAGCAGGTG CAGAAGTACA AGCAGGTGTT CATCGAAGCC
    GTGGCCAAAG CCAGGAAGTT GGACGAGGCC TTGTCCCAGT ACTTCGGGGC GGCTACAGAG
    GTAGCCCCAC AGGACGCCCT CCCGGCTGTG CCCGAGCCCA TCAGGAAGTG TCCTCAGTGC
    AGCCGGGACA TGGTTCTTAA GACCAGAAAG AGTGGCGGGT TTTACCTCGG TTGCACGGGC
    TTCCCTGAGT GTCGGTCGGC TGTCTGGTTC CCGGACAGCG TGCTGGAGGC CAGCAGGGAT
    GTGAGCGTGT GCCCAGTGTG CCAGCCGCAC CCAGTTTACA GGTTGAAGTT CAAGTTTAAA
    CGAGGCAGCC TGCCCCCAAC CATGCCCCTA GAATTTGTCG GCTGCATCGG CGGGTGTGAT
    GAGACCCTGA GGGAGATCCT GGCCCTCAGG TTCTCCCAGG GCGCTGCCCG AGTCAGCCAG
    CCTGCCAGCC AGACCTCCGG CTACCTGCAG GCTAGCCAGT CCCTGAACAG AATGGACAGT
    GGCCAGCAGG GCCAGCCCCG GCCCCCCGTC ACCAGGCCCT CGAAGGCTTT GACCAGGACT
    CTTCCTCCAC CCGCCGTTGA GAGTGAGAGC AACTCGGTGA CCTGCAACTG TGGCCAGGAG
    GCCCTGCTGC TCACCGTCCG GAAGGAGGGC CCCAACCAGG GCCGCCAGTT CTACAAGTGT
    GGCAGTGGTG GCTGCAGCTT CTTTCTATGG GCTGACAGCG GCCACCCAGG AGCCGGGCCT
    CCCTCCTCAG CGTCCAGACC CCCAGGCGGC CCCCTGGAAC ACCCCCCGGG CTCAGGGACT
    CTCTTGGGCG GGTCCAGCAG GCCTGGCAGT GGTGGCAGCG GTGGCGCATC CTGCCTGTGT
    GGCCAGCCGG CTGTCACGCG CACCGTGCAG AAGGAGGGGC CCAACCAGGG CCGCCAGTTC
    CACACGTGTG CCCAGCCCCG TGAGCACCAG TGCGGCTTCT TCCAGTGGGT GGACGAGAAC
    GTGGCCCCAG GGACCTTCGG AGGCCCCCTC TGGCCAGGAA GCAGGAGAGG AACCCAGGGG
    CTGGAGGCCA GAAGCAAGAG GCCTCGGGCC GGCTCCTCAG ACACAGGGTC TGCAACCAAG
    AAACCCCGGA AGTGCAGCCT TTGCCACCAG CCTGGACATA CCCGTCCCTT CTGTCCTCAG
    AACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.