TBC1D8 cDNA ORF clone, Bos taurus(cattle)

The following TBC1D8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220419 XM_024998792.1
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa239069 NM_001206142.3
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220413 XM_010809738.3
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220414 XM_024998788.1
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220415 XM_024998789.1
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220416 XM_024998790.1
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220417 XM_010809739.3
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220418 XM_024998791.1
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa51323 XM_010809739.2
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa01676 NM_001206142.1
Latest version!
Bos taurus TBC1 domain family, member 8 (with GRAM domain) (TBC1D8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa216378 NM_001206142.2
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa51322 XM_010809738.2
Latest version!
Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220419
    Clone ID Related Accession (Same CDS sequence) XM_024998792.1
    Accession Version XM_024998792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGTTAA TTTCCAGATG CCCTGTGCAG ACAATCTTAT TGTATTATAT ATTTAAACTT 
    GTCATCCCTT GGGTTGACGT CCAGAGGTTG GAGAGGACAT CCAATGTCTT TCTGACGGAT
    ACCATACGCA TCACCACCCA GAACAAGGAG CGGGACTTCT CCATGTTTCT AAACCTGGAC
    GAGGTGTTCA AGATCATGGA GCAGCTGGCC GACGTGACGC TGCGGAGGCT GCTGGACAAT
    GAGGGCTTCG ACCTGGACCC GGGTCTGCAG GAGCCCAGCC AGATCACCAA GAGGGACCTG
    GAAGCTAGGG CACAGAATGA GTTCTTCCGG GCCTTCTTCA GGCTGCCAAG GCGGGAGAAG
    CTGCACGCCG TCCTGGACTG TTCCCTCTGG ACCCCATTCA GCCGCTGTCA CACCGCCGGG
    CAGATGTTCA CCTCCGACAG TTACATCTGC TTTGCCAGCA AGGAGGCCGG CTGCTGCAAA
    GTCATCCTGC CGCTCAGAGA GGTTGTGAGC ATCGAGAAAA TGGAGGACAC GAGTCTGCTG
    CCAAACCCCA TCATCGTCAG CATCCGGAGC AAGATGGCCT TCCAGTTCAT TGAGCTCAGA
    GATCGTGACA GCCTGGTGGA GGGTCTGCTG GCGAGACTGA AGCTGGTGCA TGCCGACCAC
    CCTGTGCACT ATGACACCAC CCTGGATGAG GAGCTGACCT CACCCGTGTT TCATTCAACA
    AGCTTGTGCA GTGGCAGCCA CAGGTTTGGG GGTCTTGAGA TGGTGTCTTC GCAAAATAGC
    GAGGAAAGGG AGAAAGAAAC GAGCCCACGG ATGCACCCCG AGGCTTTCAG GCAGTCGGGC
    AGCCAGAGCC CGGACTCGGG ATTGTCCAGG GAACAGATCA AGGTGAGCCT CTGGAAAGAC
    CACTTTGTGG AGTACGGCAG AACAGTGTGC ATGTTCCGCA CAGAGAAGAT CCGGAAGCTG
    GTGGCCATGG GGATCCCCGA GTCCCTGCGC GGCAGACTCT GGCTTCTTTT CTCAGATGCC
    GTCACGGACC TTGCCGCGCA TCCGGGTTAC TACGGCAACC TGGTGGAGGA GTCCATGGGA
    AAGTGTTGCC TGGTGACGGA GGAGATAGAG CGGGACCTGC ACCGTTCCTT GCCGGAGCAC
    CCCGCCTTCC AAAACGAAAC GGGCATCGCA GCGTTGAGGA GAGTCCTGAC GGCCTATGCC
    CACAGGAACC CCAAGATTGG GTACTGCCAG TCCATGAACA TCCTGACCTC CGTTCTGCTG
    CTGTATGCTA AGGAGGAGGA AGCCTTCTGG CTGCTGGTGG CTGTGTGTGA GCGGATGCTG
    CCCGACTACT TCAACCACCG AGTCATTGGG GCCCAGGTGG ACCAGTCCGT CTTCGAGGAG
    CTCATCAAGG AGCAGCTCCC TGAGCTGGCG GAGCACATGC ACGACCTCTC GGCCCTGGCG
    TCCATCTCCC TGTCCTGGTT CCTCACGCTC TTCCTCAGCA TCATGCCCCT GGAGAGTGCC
    GTGAACGTGG TCGACTGCTT CTTCTACGAT GGCATCAAGG CCATCTTCCA GCTGGGACTG
    GCTGTGTTGG AGGCCACTGC CGAGGGCCTG TGCAGCAGCA AGGATGACAG CCAGGCCTTC
    ATGGTCCTCA GCAGGTTTCT GGACCACGTC AAGAACGAGG ACAGCCCGGG ACCCCCGATT
    GGCAGCCACC ACGCCTTCTT CTCTGACGAC CAGGAGCCGT GTCCTGTGAC CGACATCGCA
    GACCTGATCC GGGACTCCTA CGAGAAATTT GGGGACCAGT CTGTGGCTCA GATCGAGCAC
    ATGCGCTGCA GGCACAGGAT CCGAGTCCTC CAGGGCCACG AGGACACCAC GAAGCAGAAC
    GTGCTCCGCG TCGTTATCCC AGAAGTCTCT GTCCTTCCTG AAGACCTGGA GGAGCTCTAC
    GATTTATTCA AGAGAGAACA CATGATGAGC TGCTACTGGG AGCAGCCTCG GCCCATGGCC
    CCACGCCACG ACCCCAGCAG GCCCTACGCA GAGCAGTACC GCATAGATGC CCAGCAGTTT
    GCACGCCTGT TCCAGCTCGT CTCGCCGTGG ACCTGCGGGG CCCACACGGA AATCCTCGCA
    GAAAGGACTT TCAGGCTTCT GGACGAAAAC ATGGACCACC TTATCGAATT CAAAGCATTC
    GTGAGCTGCC TCGATATTAT GTATAATGGA GAAATGAATG AGAAAATTAA ACTGTTATAT
    CGGCTTCATA TCCCTCCAGC ACTTACTGAA AATGACCGAG ACAGCCAGTC ACCACTGAAG
    AACCCTCTGT TGTCGACGTC AAGACCCCTG GTTTTCGGCA AGTCCAATGG TGATACAATT
    GATTATCAGA AACAGCTGAA GCAGATGATT AAAGATTTGG CCAAAGAGAA AGACAAAACT
    GAGAAAGAGT TGCCCAAAAT GAGCCAGAGA GAGTTTATTC AGTTCTGTAA GACTTTGTAC
    AGTATGTTCC ACGAAGACCC GGAAGAGAAT GACCTGTACC AAGCCATCGC CACAGTAACG
    ACGCTGCTGC TGCAGATTGG GGAGGTGGGG CAGCGCGGCA GCAGCTCTGG GAGCGGCTCC
    CAGGAAGGCG GGGAGGAGCT GCAGGCCTCG GCTCCTTCCC CCTCTTCTGA GCAGGACTTG
    GTGTTTGCCG AGACTGACAC AGGGCAGAGT CCTCAGGCGT CCCAGGCATT TCCTGAGGAG
    GCACAAGGGG ACTGGACTGT CTCCCTTGAA CATATTTTAG CCTCACTTCT GACTGAACAG
    TCACTAGTCA ACTTTTTTGA AAAGCCACTG GACATTAAAT CCAAACTTGA AAACGCCAAG
    CTCAATCAGT ACAGCCTCAA GACTTGTGAA ATGAGCCGCC AGTCACAGCC TGAGCTCAAG
    CTGAGCAGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854560.1
    CDS88..2982
    Translation

    Target ORF information:

    RefSeq Version XM_024998792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGTTAA TTTCCAGATG CCCTGTGCAG ACAATCTTAT TGTATTATAT ATTTAAACTT 
    GTCATCCCTT GGGTTGACGT CCAGAGGTTG GAGAGGACAT CCAATGTCTT TCTGACGGAT
    ACCATACGCA TCACCACCCA GAACAAGGAG CGGGACTTCT CCATGTTTCT AAACCTGGAC
    GAGGTGTTCA AGATCATGGA GCAGCTGGCC GACGTGACGC TGCGGAGGCT GCTGGACAAT
    GAGGGCTTCG ACCTGGACCC GGGTCTGCAG GAGCCCAGCC AGATCACCAA GAGGGACCTG
    GAAGCTAGGG CACAGAATGA GTTCTTCCGG GCCTTCTTCA GGCTGCCAAG GCGGGAGAAG
    CTGCACGCCG TCCTGGACTG TTCCCTCTGG ACCCCATTCA GCCGCTGTCA CACCGCCGGG
    CAGATGTTCA CCTCCGACAG TTACATCTGC TTTGCCAGCA AGGAGGCCGG CTGCTGCAAA
    GTCATCCTGC CGCTCAGAGA GGTTGTGAGC ATCGAGAAAA TGGAGGACAC GAGTCTGCTG
    CCAAACCCCA TCATCGTCAG CATCCGGAGC AAGATGGCCT TCCAGTTCAT TGAGCTCAGA
    GATCGTGACA GCCTGGTGGA GGGTCTGCTG GCGAGACTGA AGCTGGTGCA TGCCGACCAC
    CCTGTGCACT ATGACACCAC CCTGGATGAG GAGCTGACCT CACCCGTGTT TCATTCAACA
    AGCTTGTGCA GTGGCAGCCA CAGGTTTGGG GGTCTTGAGA TGGTGTCTTC GCAAAATAGC
    GAGGAAAGGG AGAAAGAAAC GAGCCCACGG ATGCACCCCG AGGCTTTCAG GCAGTCGGGC
    AGCCAGAGCC CGGACTCGGG ATTGTCCAGG GAACAGATCA AGGTGAGCCT CTGGAAAGAC
    CACTTTGTGG AGTACGGCAG AACAGTGTGC ATGTTCCGCA CAGAGAAGAT CCGGAAGCTG
    GTGGCCATGG GGATCCCCGA GTCCCTGCGC GGCAGACTCT GGCTTCTTTT CTCAGATGCC
    GTCACGGACC TTGCCGCGCA TCCGGGTTAC TACGGCAACC TGGTGGAGGA GTCCATGGGA
    AAGTGTTGCC TGGTGACGGA GGAGATAGAG CGGGACCTGC ACCGTTCCTT GCCGGAGCAC
    CCCGCCTTCC AAAACGAAAC GGGCATCGCA GCGTTGAGGA GAGTCCTGAC GGCCTATGCC
    CACAGGAACC CCAAGATTGG GTACTGCCAG TCCATGAACA TCCTGACCTC CGTTCTGCTG
    CTGTATGCTA AGGAGGAGGA AGCCTTCTGG CTGCTGGTGG CTGTGTGTGA GCGGATGCTG
    CCCGACTACT TCAACCACCG AGTCATTGGG GCCCAGGTGG ACCAGTCCGT CTTCGAGGAG
    CTCATCAAGG AGCAGCTCCC TGAGCTGGCG GAGCACATGC ACGACCTCTC GGCCCTGGCG
    TCCATCTCCC TGTCCTGGTT CCTCACGCTC TTCCTCAGCA TCATGCCCCT GGAGAGTGCC
    GTGAACGTGG TCGACTGCTT CTTCTACGAT GGCATCAAGG CCATCTTCCA GCTGGGACTG
    GCTGTGTTGG AGGCCACTGC CGAGGGCCTG TGCAGCAGCA AGGATGACAG CCAGGCCTTC
    ATGGTCCTCA GCAGGTTTCT GGACCACGTC AAGAACGAGG ACAGCCCGGG ACCCCCGATT
    GGCAGCCACC ACGCCTTCTT CTCTGACGAC CAGGAGCCGT GTCCTGTGAC CGACATCGCA
    GACCTGATCC GGGACTCCTA CGAGAAATTT GGGGACCAGT CTGTGGCTCA GATCGAGCAC
    ATGCGCTGCA GGCACAGGAT CCGAGTCCTC CAGGGCCACG AGGACACCAC GAAGCAGAAC
    GTGCTCCGCG TCGTTATCCC AGAAGTCTCT GTCCTTCCTG AAGACCTGGA GGAGCTCTAC
    GATTTATTCA AGAGAGAACA CATGATGAGC TGCTACTGGG AGCAGCCTCG GCCCATGGCC
    CCACGCCACG ACCCCAGCAG GCCCTACGCA GAGCAGTACC GCATAGATGC CCAGCAGTTT
    GCACGCCTGT TCCAGCTCGT CTCGCCGTGG ACCTGCGGGG CCCACACGGA AATCCTCGCA
    GAAAGGACTT TCAGGCTTCT GGACGAAAAC ATGGACCACC TTATCGAATT CAAAGCATTC
    GTGAGCTGCC TCGATATTAT GTATAATGGA GAAATGAATG AGAAAATTAA ACTGTTATAT
    CGGCTTCATA TCCCTCCAGC ACTTACTGAA AATGACCGAG ACAGCCAGTC ACCACTGAAG
    AACCCTCTGT TGTCGACGTC AAGACCCCTG GTTTTCGGCA AGTCCAATGG TGATACAATT
    GATTATCAGA AACAGCTGAA GCAGATGATT AAAGATTTGG CCAAAGAGAA AGACAAAACT
    GAGAAAGAGT TGCCCAAAAT GAGCCAGAGA GAGTTTATTC AGTTCTGTAA GACTTTGTAC
    AGTATGTTCC ACGAAGACCC GGAAGAGAAT GACCTGTACC AAGCCATCGC CACAGTAACG
    ACGCTGCTGC TGCAGATTGG GGAGGTGGGG CAGCGCGGCA GCAGCTCTGG GAGCGGCTCC
    CAGGAAGGCG GGGAGGAGCT GCAGGCCTCG GCTCCTTCCC CCTCTTCTGA GCAGGACTTG
    GTGTTTGCCG AGACTGACAC AGGGCAGAGT CCTCAGGCGT CCCAGGCATT TCCTGAGGAG
    GCACAAGGGG ACTGGACTGT CTCCCTTGAA CATATTTTAG CCTCACTTCT GACTGAACAG
    TCACTAGTCA ACTTTTTTGA AAAGCCACTG GACATTAAAT CCAAACTTGA AAACGCCAAG
    CTCAATCAGT ACAGCCTCAA GACTTGTGAA ATGAGCCGCC AGTCACAGCC TGAGCTCAAG
    CTGAGCAGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239069
    Clone ID Related Accession (Same CDS sequence) NM_001206142.3
    Accession Version NM_001206142.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On May 30, 2018 this sequence version replaced NM_001206142.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.8126.1, SRR6764281.27458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCCGAGCAGG AGGAGGAGCC TGAGAAATTC
    CGCGAGGCCT TGGTGAAGTT CGAGACCAGG TTCAACTTCC CGGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA AGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TTGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CTGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193071.2
    CDS135..3563
    Translation

    Target ORF information:

    RefSeq Version NM_001206142.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206142.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCCGAGCAGG AGGAGGAGCC TGAGAAATTC
    CGCGAGGCCT TGGTGAAGTT CGAGACCAGG TTCAACTTCC CGGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA AGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TTGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CTGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220413
    Clone ID Related Accession (Same CDS sequence) XM_010809738.3
    Accession Version XM_010809738.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GAGTTACTGA GCGGATCGGA CGTTTACTGG GCCGTGGCCA CGGGTGCCAC GTTAGAGGAA
    ATAAACCAGC ACTGGGACTG GCTGGAGCAA AATCTCCTCC ACACCTTGTC TGTCTTTGAT
    AATAAAGATG ACATTGCCAG TTTTGTCAAA GGGAAGGTGA AGGCTCTGAT TGCCGAGGAG
    ACGAGCAGCA GGCTGGCCGA GCAGGAGGAG GAGCCTGAGA AATTCCGCGA GGCCTTGGTG
    AAGTTCGAGA CCAGGTTCAA CTTCCCGGAA GCTGAGAAGC TGATCACATA TTACTCATGC
    TGCTGCTGGA AGGGCCGGGT GCCCCGCCAA GGCTGGCTGT ACCTGAGCAT CAACCACCTC
    TGCTTCTATT CCTTCTTCTT GGGCAAGGAG CTTAAACTTG TCATCCCTTG GGTTGACGTC
    CAGAGGTTGG AGAGGACATC CAATGTCTTT CTGACGGATA CCATACGCAT CACCACCCAG
    AACAAGGAGC GGGACTTCTC CATGTTTCTA AACCTGGACG AGGTGTTCAA GATCATGGAG
    CAGCTGGCCG ACGTGACGCT GCGGAGGCTG CTGGACAATG AGGGCTTCGA CCTGGACCCG
    GGTCTGCAGG AGCCCAGCCA GATCACCAAG AGGGACCTGG AAGCTAGGGC ACAGAATGAG
    TTCTTCCGGG CCTTCTTCAG GCTGCCAAGG CGGGAGAAGC TGCACGCCGT CCTGGACTGT
    TCCCTCTGGA CCCCATTCAG CCGCTGTCAC ACCGCCGGGC AGATGTTCAC CTCCGACAGT
    TACATCTGCT TTGCCAGCAA GGAGGCCGGC TGCTGCAAAG TCATCCTGCC GCTCAGAGAG
    GTTGTGAGCA TCGAGAAAAT GGAGGACACG AGTCTGCTGC CAAACCCCAT CATCGTCAGC
    ATCCGGAGCA AGATGGCCTT CCAGTTCATT GAGCTCAGAG ATCGTGACAG CCTGGTGGAG
    GGTCTGCTGG CGAGACTGAA GCTGGTGCAT GCCGACCACC CTGTGCACTA TGACACCACC
    CTGGATGAGG AGCTGACCTC ACCCGTGTTT CATTCAACAA GCTTGTGCAG TGGCAGCCAC
    AGGTTTGGGG GTCTTGAGAT GGTGTCTTCG CAAAATAGCG AGGAAAGGGA GAAAGAAACG
    AGCCCACGGA TGCACCCCGA GGCTTTCAGG CAGTCGGGCA GCCAGAGCCC GGACTCGGGA
    TTGTCCAGGG AACAGATCAA GGTGAGCCTC TGGAAAGACC ACTTTGTGGA GTACGGCAGA
    ACAGTGTGCA TGTTCCGCAC AGAGAAGATC CGGAAGCTGG TGGCCATGGG GATCCCCGAG
    TCCCTGCGCG GCAGACTCTG GCTTCTTTTC TCAGATGCCG TCACGGACCT TGCCGCGCAT
    CCGGGTTACT ACGGCAACCT GGTGGAGGAG TCCATGGGAA AGTGTTGCCT GGTGACGGAG
    GAGATAGAGC GGGACCTGCA CCGTTCCTTG CCGGAGCACC CCGCCTTCCA AAACGAAACG
    GGCATCGCAG CGTTGAGGAG AGTCCTGACG GCCTATGCCC ACAGGAACCC CAAGATTGGG
    TACTGCCAGT CCATGAACAT CCTGACCTCC GTTCTGCTGC TGTATGCTAA GGAGGAGGAA
    GCCTTCTGGC TGCTGGTGGC TGTGTGTGAG CGGATGCTGC CCGACTACTT CAACCACCGA
    GTCATTGGGG CCCAGGTGGA CCAGTCCGTC TTCGAGGAGC TCATCAAGGA GCAGCTCCCT
    GAGCTGGCGG AGCACATGCA CGACCTCTCG GCCCTGGCGT CCATCTCCCT GTCCTGGTTC
    CTCACGCTCT TCCTCAGCAT CATGCCCCTG GAGAGTGCCG TGAACGTGGT CGACTGCTTC
    TTCTACGATG GCATCAAGGC CATCTTCCAG CTGGGACTGG CTGTGTTGGA GGCCACTGCC
    GAGGGCCTGT GCAGCAGCAA GGATGACAGC CAGGCCTTCA TGGTCCTCAG CAGGTTTCTG
    GACCACGTCA AGAACGAGGA CAGCCCGGGA CCCCCGATTG GCAGCCACCA CGCCTTCTTC
    TCTGACGACC AGGAGCCGTG TCCTGTGACC GACATCGCAG ACCTGATCCG GGACTCCTAC
    GAGAAATTTG GGGACCAGTC TGTGGCTCAG ATCGAGCACA TGCGCTGCAG GCACAGGATC
    CGAGTCCTCC AGGGCCACGA GGACACCACG AAGCAGAACG TGCTCCGCGT CGTTATCCCA
    GAAGTCTCTG TCCTTCCTGA AGACCTGGAG GAGCTCTACG ATTTATTCAA GAGAGAACAC
    ATGATGAGCT GCTACTGGGA GCAGCCTCGG CCCATGGCCC CACGCCACGA CCCCAGCAGG
    CCCTACGCAG AGCAGTACCG CATAGATGCC CAGCAGTTTG CACGCCTGTT CCAGCTCGTC
    TCGCCGTGGA CCTGCGGGGC CCACACGGAA ATCCTCGCAG AAAGGACTTT CAGGCTTCTG
    GACGAAAACA TGGACCACCT TATCGAATTC AAAGCATTCG TGAGCTGCCT CGATATTATG
    TATAATGGAG AAATGAATGA GAAAATTAAA CTGTTATATC GGCTTCATAT CCCTCCAGCA
    CTTACTGAAA ATGACCGAGA CAGCCAGTCA CCACTGAAGA ACCCTCTGTT GTCGACGTCA
    AGACCCCTGG TTTTCGGCAA GTCCAATGGT GATACAATTG ATTATCAGAA ACAGCTGAAG
    CAGATGATTA AAGATTTGGC CAAAGAGAAA GACAAAACTG AGAAAGAGTT GCCCAAAATG
    AGCCAGAGAG AGTTTATTCA GTTCTGTAAG ACTTTGTACA GTATGTTCCA CGAAGACCCG
    GAAGAGAATG ACCTGTACCA AGCCATCGCC ACAGTAACGA CGCTGCTGCT GCAGATTGGG
    GAGGTGGGGC AGCGCGGCAG CAGCTCTGGG AGCGGCTCCC AGGAAGGCGG GGAGGAGCTG
    CAGGCCTCGG CTCCTTCCCC CTCTTCTGAG CAGGACTTGG TGTTTGCCGA GACTGACACA
    GGGCAGAGTC CTCAGGCGTC CCAGGCATTT CCTGAGGAGG CACAAGGGGA CTGGACTGTC
    TCCCTTGAAC ATATTTTAGC CTCACTTCTG ACTGAACAGT CACTAGTCAA CTTTTTTGAA
    AAGCCACTGG ACATTAAATC CAAACTTGAA AACGCCAAGC TCAATCAGTA CAGCCTCAAG
    ACTTGTGAAA TGAGCCGCCA GTCACAGCCT GAGCTCAAGC TGAGCAGCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808040.2
    CDS1..3474
    Translation

    Target ORF information:

    RefSeq Version XM_010809738.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809738.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GAGTTACTGA GCGGATCGGA CGTTTACTGG GCCGTGGCCA CGGGTGCCAC GTTAGAGGAA
    ATAAACCAGC ACTGGGACTG GCTGGAGCAA AATCTCCTCC ACACCTTGTC TGTCTTTGAT
    AATAAAGATG ACATTGCCAG TTTTGTCAAA GGGAAGGTGA AGGCTCTGAT TGCCGAGGAG
    ACGAGCAGCA GGCTGGCCGA GCAGGAGGAG GAGCCTGAGA AATTCCGCGA GGCCTTGGTG
    AAGTTCGAGA CCAGGTTCAA CTTCCCGGAA GCTGAGAAGC TGATCACATA TTACTCATGC
    TGCTGCTGGA AGGGCCGGGT GCCCCGCCAA GGCTGGCTGT ACCTGAGCAT CAACCACCTC
    TGCTTCTATT CCTTCTTCTT GGGCAAGGAG CTTAAACTTG TCATCCCTTG GGTTGACGTC
    CAGAGGTTGG AGAGGACATC CAATGTCTTT CTGACGGATA CCATACGCAT CACCACCCAG
    AACAAGGAGC GGGACTTCTC CATGTTTCTA AACCTGGACG AGGTGTTCAA GATCATGGAG
    CAGCTGGCCG ACGTGACGCT GCGGAGGCTG CTGGACAATG AGGGCTTCGA CCTGGACCCG
    GGTCTGCAGG AGCCCAGCCA GATCACCAAG AGGGACCTGG AAGCTAGGGC ACAGAATGAG
    TTCTTCCGGG CCTTCTTCAG GCTGCCAAGG CGGGAGAAGC TGCACGCCGT CCTGGACTGT
    TCCCTCTGGA CCCCATTCAG CCGCTGTCAC ACCGCCGGGC AGATGTTCAC CTCCGACAGT
    TACATCTGCT TTGCCAGCAA GGAGGCCGGC TGCTGCAAAG TCATCCTGCC GCTCAGAGAG
    GTTGTGAGCA TCGAGAAAAT GGAGGACACG AGTCTGCTGC CAAACCCCAT CATCGTCAGC
    ATCCGGAGCA AGATGGCCTT CCAGTTCATT GAGCTCAGAG ATCGTGACAG CCTGGTGGAG
    GGTCTGCTGG CGAGACTGAA GCTGGTGCAT GCCGACCACC CTGTGCACTA TGACACCACC
    CTGGATGAGG AGCTGACCTC ACCCGTGTTT CATTCAACAA GCTTGTGCAG TGGCAGCCAC
    AGGTTTGGGG GTCTTGAGAT GGTGTCTTCG CAAAATAGCG AGGAAAGGGA GAAAGAAACG
    AGCCCACGGA TGCACCCCGA GGCTTTCAGG CAGTCGGGCA GCCAGAGCCC GGACTCGGGA
    TTGTCCAGGG AACAGATCAA GGTGAGCCTC TGGAAAGACC ACTTTGTGGA GTACGGCAGA
    ACAGTGTGCA TGTTCCGCAC AGAGAAGATC CGGAAGCTGG TGGCCATGGG GATCCCCGAG
    TCCCTGCGCG GCAGACTCTG GCTTCTTTTC TCAGATGCCG TCACGGACCT TGCCGCGCAT
    CCGGGTTACT ACGGCAACCT GGTGGAGGAG TCCATGGGAA AGTGTTGCCT GGTGACGGAG
    GAGATAGAGC GGGACCTGCA CCGTTCCTTG CCGGAGCACC CCGCCTTCCA AAACGAAACG
    GGCATCGCAG CGTTGAGGAG AGTCCTGACG GCCTATGCCC ACAGGAACCC CAAGATTGGG
    TACTGCCAGT CCATGAACAT CCTGACCTCC GTTCTGCTGC TGTATGCTAA GGAGGAGGAA
    GCCTTCTGGC TGCTGGTGGC TGTGTGTGAG CGGATGCTGC CCGACTACTT CAACCACCGA
    GTCATTGGGG CCCAGGTGGA CCAGTCCGTC TTCGAGGAGC TCATCAAGGA GCAGCTCCCT
    GAGCTGGCGG AGCACATGCA CGACCTCTCG GCCCTGGCGT CCATCTCCCT GTCCTGGTTC
    CTCACGCTCT TCCTCAGCAT CATGCCCCTG GAGAGTGCCG TGAACGTGGT CGACTGCTTC
    TTCTACGATG GCATCAAGGC CATCTTCCAG CTGGGACTGG CTGTGTTGGA GGCCACTGCC
    GAGGGCCTGT GCAGCAGCAA GGATGACAGC CAGGCCTTCA TGGTCCTCAG CAGGTTTCTG
    GACCACGTCA AGAACGAGGA CAGCCCGGGA CCCCCGATTG GCAGCCACCA CGCCTTCTTC
    TCTGACGACC AGGAGCCGTG TCCTGTGACC GACATCGCAG ACCTGATCCG GGACTCCTAC
    GAGAAATTTG GGGACCAGTC TGTGGCTCAG ATCGAGCACA TGCGCTGCAG GCACAGGATC
    CGAGTCCTCC AGGGCCACGA GGACACCACG AAGCAGAACG TGCTCCGCGT CGTTATCCCA
    GAAGTCTCTG TCCTTCCTGA AGACCTGGAG GAGCTCTACG ATTTATTCAA GAGAGAACAC
    ATGATGAGCT GCTACTGGGA GCAGCCTCGG CCCATGGCCC CACGCCACGA CCCCAGCAGG
    CCCTACGCAG AGCAGTACCG CATAGATGCC CAGCAGTTTG CACGCCTGTT CCAGCTCGTC
    TCGCCGTGGA CCTGCGGGGC CCACACGGAA ATCCTCGCAG AAAGGACTTT CAGGCTTCTG
    GACGAAAACA TGGACCACCT TATCGAATTC AAAGCATTCG TGAGCTGCCT CGATATTATG
    TATAATGGAG AAATGAATGA GAAAATTAAA CTGTTATATC GGCTTCATAT CCCTCCAGCA
    CTTACTGAAA ATGACCGAGA CAGCCAGTCA CCACTGAAGA ACCCTCTGTT GTCGACGTCA
    AGACCCCTGG TTTTCGGCAA GTCCAATGGT GATACAATTG ATTATCAGAA ACAGCTGAAG
    CAGATGATTA AAGATTTGGC CAAAGAGAAA GACAAAACTG AGAAAGAGTT GCCCAAAATG
    AGCCAGAGAG AGTTTATTCA GTTCTGTAAG ACTTTGTACA GTATGTTCCA CGAAGACCCG
    GAAGAGAATG ACCTGTACCA AGCCATCGCC ACAGTAACGA CGCTGCTGCT GCAGATTGGG
    GAGGTGGGGC AGCGCGGCAG CAGCTCTGGG AGCGGCTCCC AGGAAGGCGG GGAGGAGCTG
    CAGGCCTCGG CTCCTTCCCC CTCTTCTGAG CAGGACTTGG TGTTTGCCGA GACTGACACA
    GGGCAGAGTC CTCAGGCGTC CCAGGCATTT CCTGAGGAGG CACAAGGGGA CTGGACTGTC
    TCCCTTGAAC ATATTTTAGC CTCACTTCTG ACTGAACAGT CACTAGTCAA CTTTTTTGAA
    AAGCCACTGG ACATTAAATC CAAACTTGAA AACGCCAAGC TCAATCAGTA CAGCCTCAAG
    ACTTGTGAAA TGAGCCGCCA GTCACAGCCT GAGCTCAAGC TGAGCAGCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220414
    Clone ID Related Accession (Same CDS sequence) XM_024998788.1
    Accession Version XM_024998788.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGAGTTA TCTTCAGCTT CTTTATAGAA GGTCGCCTGG TGGGTGCCCT GGACGCGGTG 
    CTGGATTCCA ACGCCCGGGT TGCTCCGTTC CGAATTCTGC TCCAGGTGCC CGGCTCCCAG
    GCGTATTCTC CCATAGCATG TGGTGAGTTA CTGAGCGGAT CGGACGTTTA CTGGGCCGTG
    GCCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CCGAGCAGGA GGAGGAGCCT
    GAGAAATTCC GCGAGGCCTT GGTGAAGTTC GAGACCAGGT TCAACTTCCC GGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAA
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC TGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854556.1
    CDS16..3393
    Translation

    Target ORF information:

    RefSeq Version XM_024998788.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGAGTTA TCTTCAGCTT CTTTATAGAA GGTCGCCTGG TGGGTGCCCT GGACGCGGTG 
    CTGGATTCCA ACGCCCGGGT TGCTCCGTTC CGAATTCTGC TCCAGGTGCC CGGCTCCCAG
    GCGTATTCTC CCATAGCATG TGGTGAGTTA CTGAGCGGAT CGGACGTTTA CTGGGCCGTG
    GCCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CCGAGCAGGA GGAGGAGCCT
    GAGAAATTCC GCGAGGCCTT GGTGAAGTTC GAGACCAGGT TCAACTTCCC GGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAA
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC TGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220415
    Clone ID Related Accession (Same CDS sequence) XM_024998789.1
    Accession Version XM_024998789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGTGA GAAGTCGCCT GGTGGGTGCC CTGGACGCGG TGCTGGATTC CAACGCCCGG 
    GTTGCTCCGT TCCGAATTCT GCTCCAGGTG CCCGGCTCCC AGGCGTATTC TCCCATAGCA
    TGTGGTGAGT TACTGAGCGG ATCGGACGTT TACTGGGCCG TGGCCACGGG TGCCACGTTA
    GAGGAAATAA ACCAGCACTG GGACTGGCTG GAGCAAAATC TCCTCCACAC CTTGTCTGTC
    TTTGATAATA AAGATGACAT TGCCAGTTTT GTCAAAGGGA AGGTGAAGGC TCTGATTGCC
    GAGGAGACGA GCAGCAGGCT GGCCGAGCAG GAGGAGGAGC CTGAGAAATT CCGCGAGGCC
    TTGGTGAAGT TCGAGACCAG GTTCAACTTC CCGGAAGCTG AGAAGCTGAT CACATATTAC
    TCATGCTGCT GCTGGAAGGG CCGGGTGCCC CGCCAAGGCT GGCTGTACCT GAGCATCAAC
    CACCTCTGCT TCTATTCCTT CTTCTTGGGC AAGGAGCTTA AACTTGTCAT CCCTTGGGTT
    GACGTCCAGA GGTTGGAGAG GACATCCAAT GTCTTTCTGA CGGATACCAT ACGCATCACC
    ACCCAGAACA AGGAGCGGGA CTTCTCCATG TTTCTAAACC TGGACGAGGT GTTCAAGATC
    ATGGAGCAGC TGGCCGACGT GACGCTGCGG AGGCTGCTGG ACAATGAGGG CTTCGACCTG
    GACCCGGGTC TGCAGGAGCC CAGCCAGATC ACCAAGAGGG ACCTGGAAGC TAGGGCACAG
    AATGAGTTCT TCCGGGCCTT CTTCAGGCTG CCAAGGCGGG AGAAGCTGCA CGCCGTCCTG
    GACTGTTCCC TCTGGACCCC ATTCAGCCGC TGTCACACCG CCGGGCAGAT GTTCACCTCC
    GACAGTTACA TCTGCTTTGC CAGCAAGGAG GCCGGCTGCT GCAAAGTCAT CCTGCCGCTC
    AGAGAGGTTG TGAGCATCGA GAAAATGGAG GACACGAGTC TGCTGCCAAA CCCCATCATC
    GTCAGCATCC GGAGCAAGAT GGCCTTCCAG TTCATTGAGC TCAGAGATCG TGACAGCCTG
    GTGGAGGGTC TGCTGGCGAG ACTGAAGCTG GTGCATGCCG ACCACCCTGT GCACTATGAC
    ACCACCCTGG ATGAGGAGCT GACCTCACCC GTGTTTCATT CAACAAGCTT GTGCAGTGGC
    AGCCACAGGT TTGGGGGTCT TGAGATGGTG TCTTCGCAAA ATAGCGAGGA AAGGGAGAAA
    GAAACGAGCC CACGGATGCA CCCCGAGGCT TTCAGGCAGT CGGGCAGCCA GAGCCCGGAC
    TCGGGATTGT CCAGGGAACA GATCAAGGTG AGCCTCTGGA AAGACCACTT TGTGGAGTAC
    GGCAGAACAG TGTGCATGTT CCGCACAGAG AAGATCCGGA AGCTGGTGGC CATGGGGATC
    CCCGAGTCCC TGCGCGGCAG ACTCTGGCTT CTTTTCTCAG ATGCCGTCAC GGACCTTGCC
    GCGCATCCGG GTTACTACGG CAACCTGGTG GAGGAGTCCA TGGGAAAGTG TTGCCTGGTG
    ACGGAGGAGA TAGAGCGGGA CCTGCACCGT TCCTTGCCGG AGCACCCCGC CTTCCAAAAC
    GAAACGGGCA TCGCAGCGTT GAGGAGAGTC CTGACGGCCT ATGCCCACAG GAACCCCAAG
    ATTGGGTACT GCCAGTCCAT GAACATCCTG ACCTCCGTTC TGCTGCTGTA TGCTAAGGAG
    GAGGAAGCCT TCTGGCTGCT GGTGGCTGTG TGTGAGCGGA TGCTGCCCGA CTACTTCAAC
    CACCGAGTCA TTGGGGCCCA GGTGGACCAG TCCGTCTTCG AGGAGCTCAT CAAGGAGCAG
    CTCCCTGAGC TGGCGGAGCA CATGCACGAC CTCTCGGCCC TGGCGTCCAT CTCCCTGTCC
    TGGTTCCTCA CGCTCTTCCT CAGCATCATG CCCCTGGAGA GTGCCGTGAA CGTGGTCGAC
    TGCTTCTTCT ACGATGGCAT CAAGGCCATC TTCCAGCTGG GACTGGCTGT GTTGGAGGCC
    ACTGCCGAGG GCCTGTGCAG CAGCAAGGAT GACAGCCAGG CCTTCATGGT CCTCAGCAGG
    TTTCTGGACC ACGTCAAGAA CGAGGACAGC CCGGGACCCC CGATTGGCAG CCACCACGCC
    TTCTTCTCTG ACGACCAGGA GCCGTGTCCT GTGACCGACA TCGCAGACCT GATCCGGGAC
    TCCTACGAGA AATTTGGGGA CCAGTCTGTG GCTCAGATCG AGCACATGCG CTGCAGGCAC
    AGGATCCGAG TCCTCCAGGG CCACGAGGAC ACCACGAAGC AGAACGTGCT CCGCGTCGTT
    ATCCCAGAAG TCTCTGTCCT TCCTGAAGAC CTGGAGGAGC TCTACGATTT ATTCAAGAGA
    GAACACATGA TGAGCTGCTA CTGGGAGCAG CCTCGGCCCA TGGCCCCACG CCACGACCCC
    AGCAGGCCCT ACGCAGAGCA GTACCGCATA GATGCCCAGC AGTTTGCACG CCTGTTCCAG
    CTCGTCTCGC CGTGGACCTG CGGGGCCCAC ACGGAAATCC TCGCAGAAAG GACTTTCAGG
    CTTCTGGACG AAAACATGGA CCACCTTATC GAATTCAAAG CATTCGTGAG CTGCCTCGAT
    ATTATGTATA ATGGAGAAAT GAATGAGAAA ATTAAACTGT TATATCGGCT TCATATCCCT
    CCAGCACTTA CTGAAAATGA CCGAGACAGC CAGTCACCAC TGAAGAACCC TCTGTTGTCG
    ACGTCAAGAC CCCTGGTTTT CGGCAAGTCC AATGGTGATA CAATTGATTA TCAGAAACAG
    CTGAAGCAGA TGATTAAAGA TTTGGCCAAA GAGAAAGACA AAACTGAGAA AGAGTTGCCC
    AAAATGAGCC AGAGAGAGTT TATTCAGTTC TGTAAGACTT TGTACAGTAT GTTCCACGAA
    GACCCGGAAG AGAATGACCT GTACCAAGCC ATCGCCACAG TAACGACGCT GCTGCTGCAG
    ATTGGGGAGG TGGGGCAGCG CGGCAGCAGC TCTGGGAGCG GCTCCCAGGA AGGCGGGGAG
    GAGCTGCAGG CCTCGGCTCC TTCCCCCTCT TCTGAGCAGG ACTTGGTGTT TGCCGAGACT
    GACACAGGGC AGAGTCCTCA GGCGTCCCAG GCATTTCCTG AGGAGGCACA AGGGGACTGG
    ACTGTCTCCC TTGAACATAT TTTAGCCTCA CTTCTGACTG AACAGTCACT AGTCAACTTT
    TTTGAAAAGC CACTGGACAT TAAATCCAAA CTTGAAAACG CCAAGCTCAA TCAGTACAGC
    CTCAAGACTT GTGAAATGAG CCGCCAGTCA CAGCCTGAGC TCAAGCTGAG CAGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854557.1
    CDS308..3667
    Translation

    Target ORF information:

    RefSeq Version XM_024998789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGTGA GAAGTCGCCT GGTGGGTGCC CTGGACGCGG TGCTGGATTC CAACGCCCGG 
    GTTGCTCCGT TCCGAATTCT GCTCCAGGTG CCCGGCTCCC AGGCGTATTC TCCCATAGCA
    TGTGGTGAGT TACTGAGCGG ATCGGACGTT TACTGGGCCG TGGCCACGGG TGCCACGTTA
    GAGGAAATAA ACCAGCACTG GGACTGGCTG GAGCAAAATC TCCTCCACAC CTTGTCTGTC
    TTTGATAATA AAGATGACAT TGCCAGTTTT GTCAAAGGGA AGGTGAAGGC TCTGATTGCC
    GAGGAGACGA GCAGCAGGCT GGCCGAGCAG GAGGAGGAGC CTGAGAAATT CCGCGAGGCC
    TTGGTGAAGT TCGAGACCAG GTTCAACTTC CCGGAAGCTG AGAAGCTGAT CACATATTAC
    TCATGCTGCT GCTGGAAGGG CCGGGTGCCC CGCCAAGGCT GGCTGTACCT GAGCATCAAC
    CACCTCTGCT TCTATTCCTT CTTCTTGGGC AAGGAGCTTA AACTTGTCAT CCCTTGGGTT
    GACGTCCAGA GGTTGGAGAG GACATCCAAT GTCTTTCTGA CGGATACCAT ACGCATCACC
    ACCCAGAACA AGGAGCGGGA CTTCTCCATG TTTCTAAACC TGGACGAGGT GTTCAAGATC
    ATGGAGCAGC TGGCCGACGT GACGCTGCGG AGGCTGCTGG ACAATGAGGG CTTCGACCTG
    GACCCGGGTC TGCAGGAGCC CAGCCAGATC ACCAAGAGGG ACCTGGAAGC TAGGGCACAG
    AATGAGTTCT TCCGGGCCTT CTTCAGGCTG CCAAGGCGGG AGAAGCTGCA CGCCGTCCTG
    GACTGTTCCC TCTGGACCCC ATTCAGCCGC TGTCACACCG CCGGGCAGAT GTTCACCTCC
    GACAGTTACA TCTGCTTTGC CAGCAAGGAG GCCGGCTGCT GCAAAGTCAT CCTGCCGCTC
    AGAGAGGTTG TGAGCATCGA GAAAATGGAG GACACGAGTC TGCTGCCAAA CCCCATCATC
    GTCAGCATCC GGAGCAAGAT GGCCTTCCAG TTCATTGAGC TCAGAGATCG TGACAGCCTG
    GTGGAGGGTC TGCTGGCGAG ACTGAAGCTG GTGCATGCCG ACCACCCTGT GCACTATGAC
    ACCACCCTGG ATGAGGAGCT GACCTCACCC GTGTTTCATT CAACAAGCTT GTGCAGTGGC
    AGCCACAGGT TTGGGGGTCT TGAGATGGTG TCTTCGCAAA ATAGCGAGGA AAGGGAGAAA
    GAAACGAGCC CACGGATGCA CCCCGAGGCT TTCAGGCAGT CGGGCAGCCA GAGCCCGGAC
    TCGGGATTGT CCAGGGAACA GATCAAGGTG AGCCTCTGGA AAGACCACTT TGTGGAGTAC
    GGCAGAACAG TGTGCATGTT CCGCACAGAG AAGATCCGGA AGCTGGTGGC CATGGGGATC
    CCCGAGTCCC TGCGCGGCAG ACTCTGGCTT CTTTTCTCAG ATGCCGTCAC GGACCTTGCC
    GCGCATCCGG GTTACTACGG CAACCTGGTG GAGGAGTCCA TGGGAAAGTG TTGCCTGGTG
    ACGGAGGAGA TAGAGCGGGA CCTGCACCGT TCCTTGCCGG AGCACCCCGC CTTCCAAAAC
    GAAACGGGCA TCGCAGCGTT GAGGAGAGTC CTGACGGCCT ATGCCCACAG GAACCCCAAG
    ATTGGGTACT GCCAGTCCAT GAACATCCTG ACCTCCGTTC TGCTGCTGTA TGCTAAGGAG
    GAGGAAGCCT TCTGGCTGCT GGTGGCTGTG TGTGAGCGGA TGCTGCCCGA CTACTTCAAC
    CACCGAGTCA TTGGGGCCCA GGTGGACCAG TCCGTCTTCG AGGAGCTCAT CAAGGAGCAG
    CTCCCTGAGC TGGCGGAGCA CATGCACGAC CTCTCGGCCC TGGCGTCCAT CTCCCTGTCC
    TGGTTCCTCA CGCTCTTCCT CAGCATCATG CCCCTGGAGA GTGCCGTGAA CGTGGTCGAC
    TGCTTCTTCT ACGATGGCAT CAAGGCCATC TTCCAGCTGG GACTGGCTGT GTTGGAGGCC
    ACTGCCGAGG GCCTGTGCAG CAGCAAGGAT GACAGCCAGG CCTTCATGGT CCTCAGCAGG
    TTTCTGGACC ACGTCAAGAA CGAGGACAGC CCGGGACCCC CGATTGGCAG CCACCACGCC
    TTCTTCTCTG ACGACCAGGA GCCGTGTCCT GTGACCGACA TCGCAGACCT GATCCGGGAC
    TCCTACGAGA AATTTGGGGA CCAGTCTGTG GCTCAGATCG AGCACATGCG CTGCAGGCAC
    AGGATCCGAG TCCTCCAGGG CCACGAGGAC ACCACGAAGC AGAACGTGCT CCGCGTCGTT
    ATCCCAGAAG TCTCTGTCCT TCCTGAAGAC CTGGAGGAGC TCTACGATTT ATTCAAGAGA
    GAACACATGA TGAGCTGCTA CTGGGAGCAG CCTCGGCCCA TGGCCCCACG CCACGACCCC
    AGCAGGCCCT ACGCAGAGCA GTACCGCATA GATGCCCAGC AGTTTGCACG CCTGTTCCAG
    CTCGTCTCGC CGTGGACCTG CGGGGCCCAC ACGGAAATCC TCGCAGAAAG GACTTTCAGG
    CTTCTGGACG AAAACATGGA CCACCTTATC GAATTCAAAG CATTCGTGAG CTGCCTCGAT
    ATTATGTATA ATGGAGAAAT GAATGAGAAA ATTAAACTGT TATATCGGCT TCATATCCCT
    CCAGCACTTA CTGAAAATGA CCGAGACAGC CAGTCACCAC TGAAGAACCC TCTGTTGTCG
    ACGTCAAGAC CCCTGGTTTT CGGCAAGTCC AATGGTGATA CAATTGATTA TCAGAAACAG
    CTGAAGCAGA TGATTAAAGA TTTGGCCAAA GAGAAAGACA AAACTGAGAA AGAGTTGCCC
    AAAATGAGCC AGAGAGAGTT TATTCAGTTC TGTAAGACTT TGTACAGTAT GTTCCACGAA
    GACCCGGAAG AGAATGACCT GTACCAAGCC ATCGCCACAG TAACGACGCT GCTGCTGCAG
    ATTGGGGAGG TGGGGCAGCG CGGCAGCAGC TCTGGGAGCG GCTCCCAGGA AGGCGGGGAG
    GAGCTGCAGG CCTCGGCTCC TTCCCCCTCT TCTGAGCAGG ACTTGGTGTT TGCCGAGACT
    GACACAGGGC AGAGTCCTCA GGCGTCCCAG GCATTTCCTG AGGAGGCACA AGGGGACTGG
    ACTGTCTCCC TTGAACATAT TTTAGCCTCA CTTCTGACTG AACAGTCACT AGTCAACTTT
    TTTGAAAAGC CACTGGACAT TAAATCCAAA CTTGAAAACG CCAAGCTCAA TCAGTACAGC
    CTCAAGACTT GTGAAATGAG CCGCCAGTCA CAGCCTGAGC TCAAGCTGAG CAGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220416
    Clone ID Related Accession (Same CDS sequence) XM_024998790.1
    Accession Version XM_024998790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGAGTTA TCTTCAGCTT CTTTATAGAA GGTCGCCTGG TGGGTGCCCT GGACGCGGTG 
    CTGGATTCCA ACGCCCGGGT TGCTCCGTTC CGAATTCTGC TCCAGGTGCC CGGCTCCCAG
    GCGTATTCTC CCATAGCATG TGGTGCCACG TTAGAGGAAA TAAACCAGCA CTGGGACTGG
    CTGGAGCAAA ATCTCCTCCA CACCTTGTCT GTCTTTGATA ATAAAGATGA CATTGCCAGT
    TTTGTCAAAG GGAAGGTGAA GGCTCTGATT GCCGAGGAGA CGAGCAGCAG GCTGGCCGAG
    CAGGAGGAGG AGCCTGAGAA ATTCCGCGAG GCCTTGGTGA AGTTCGAGAC CAGGTTCAAC
    TTCCCGGAAG CTGAGAAGCT GATCACATAT TACTCATGCT GCTGCTGGAA GGGCCGGGTG
    CCCCGCCAAG GCTGGCTGTA CCTGAGCATC AACCACCTCT GCTTCTATTC CTTCTTCTTG
    GGCAAGGAGC TTAAACTTGT CATCCCTTGG GTTGACGTCC AGAGGTTGGA GAGGACATCC
    AATGTCTTTC TGACGGATAC CATACGCATC ACCACCCAGA ACAAGGAGCG GGACTTCTCC
    ATGTTTCTAA ACCTGGACGA GGTGTTCAAG ATCATGGAGC AGCTGGCCGA CGTGACGCTG
    CGGAGGCTGC TGGACAATGA GGGCTTCGAC CTGGACCCGG GTCTGCAGGA GCCCAGCCAG
    ATCACCAAGA GGGACCTGGA AGCTAGGGCA CAGAATGAGT TCTTCCGGGC CTTCTTCAGG
    CTGCCAAGGC GGGAGAAGCT GCACGCCGTC CTGGACTGTT CCCTCTGGAC CCCATTCAGC
    CGCTGTCACA CCGCCGGGCA GATGTTCACC TCCGACAGTT ACATCTGCTT TGCCAGCAAG
    GAGGCCGGCT GCTGCAAAGT CATCCTGCCG CTCAGAGAGG TTGTGAGCAT CGAGAAAATG
    GAGGACACGA GTCTGCTGCC AAACCCCATC ATCGTCAGCA TCCGGAGCAA GATGGCCTTC
    CAGTTCATTG AGCTCAGAGA TCGTGACAGC CTGGTGGAGG GTCTGCTGGC GAGACTGAAG
    CTGGTGCATG CCGACCACCC TGTGCACTAT GACACCACCC TGGATGAGGA GCTGACCTCA
    CCCGTGTTTC ATTCAACAAG CTTGTGCAGT GGCAGCCACA GGTTTGGGGG TCTTGAGATG
    GTGTCTTCGC AAAATAGCGA GGAAAGGGAG AAAGAAACGA GCCCACGGAT GCACCCCGAG
    GCTTTCAGGC AGTCGGGCAG CCAGAGCCCG GACTCGGGAT TGTCCAGGGA ACAGATCAAG
    GTGAGCCTCT GGAAAGACCA CTTTGTGGAG TACGGCAGAA CAGTGTGCAT GTTCCGCACA
    GAGAAGATCC GGAAGCTGGT GGCCATGGGG ATCCCCGAGT CCCTGCGCGG CAGACTCTGG
    CTTCTTTTCT CAGATGCCGT CACGGACCTT GCCGCGCATC CGGGTTACTA CGGCAACCTG
    GTGGAGGAGT CCATGGGAAA GTGTTGCCTG GTGACGGAGG AGATAGAGCG GGACCTGCAC
    CGTTCCTTGC CGGAGCACCC CGCCTTCCAA AACGAAACGG GCATCGCAGC GTTGAGGAGA
    GTCCTGACGG CCTATGCCCA CAGGAACCCC AAGATTGGGT ACTGCCAGTC CATGAACATC
    CTGACCTCCG TTCTGCTGCT GTATGCTAAG GAGGAGGAAG CCTTCTGGCT GCTGGTGGCT
    GTGTGTGAGC GGATGCTGCC CGACTACTTC AACCACCGAG TCATTGGGGC CCAGGTGGAC
    CAGTCCGTCT TCGAGGAGCT CATCAAGGAG CAGCTCCCTG AGCTGGCGGA GCACATGCAC
    GACCTCTCGG CCCTGGCGTC CATCTCCCTG TCCTGGTTCC TCACGCTCTT CCTCAGCATC
    ATGCCCCTGG AGAGTGCCGT GAACGTGGTC GACTGCTTCT TCTACGATGG CATCAAGGCC
    ATCTTCCAGC TGGGACTGGC TGTGTTGGAG GCCACTGCCG AGGGCCTGTG CAGCAGCAAG
    GATGACAGCC AGGCCTTCAT GGTCCTCAGC AGGTTTCTGG ACCACGTCAA GAACGAGGAC
    AGCCCGGGAC CCCCGATTGG CAGCCACCAC GCCTTCTTCT CTGACGACCA GGAGCCGTGT
    CCTGTGACCG ACATCGCAGA CCTGATCCGG GACTCCTACG AGAAATTTGG GGACCAGTCT
    GTGGCTCAGA TCGAGCACAT GCGCTGCAGG CACAGGATCC GAGTCCTCCA GGGCCACGAG
    GACACCACGA AGCAGAACGT GCTCCGCGTC GTTATCCCAG AAGTCTCTGT CCTTCCTGAA
    GACCTGGAGG AGCTCTACGA TTTATTCAAG AGAGAACACA TGATGAGCTG CTACTGGGAG
    CAGCCTCGGC CCATGGCCCC ACGCCACGAC CCCAGCAGGC CCTACGCAGA GCAGTACCGC
    ATAGATGCCC AGCAGTTTGC ACGCCTGTTC CAGCTCGTCT CGCCGTGGAC CTGCGGGGCC
    CACACGGAAA TCCTCGCAGA AAGGACTTTC AGGCTTCTGG ACGAAAACAT GGACCACCTT
    ATCGAATTCA AAGCATTCGT GAGCTGCCTC GATATTATGT ATAATGGAGA AATGAATGAG
    AAAATTAAAC TGTTATATCG GCTTCATATC CCTCCAGCAC TTACTGAAAA TGACCGAGAC
    AGCCAGTCAC CACTGAAGAA CCCTCTGTTG TCGACGTCAA GACCCCTGGT TTTCGGCAAG
    TCCAATGGTG ATACAATTGA TTATCAGAAA CAGCTGAAGC AGATGATTAA AGATTTGGCC
    AAAGAGAAAG ACAAAACTGA GAAAGAGTTG CCCAAAATGA GCCAGAGAGA GTTTATTCAG
    TTCTGTAAGA CTTTGTACAG TATGTTCCAC GAAGACCCGG AAGAGAATGA CCTGTACCAA
    GCCATCGCCA CAGTAACGAC GCTGCTGCTG CAGATTGGGG AGGTGGGGCA GCGCGGCAGC
    AGCTCTGGGA GCGGCTCCCA GGAAGGCGGG GAGGAGCTGC AGGCCTCGGC TCCTTCCCCC
    TCTTCTGAGC AGGACTTGGT GTTTGCCGAG ACTGACACAG GGCAGAGTCC TCAGGCGTCC
    CAGGCATTTC CTGAGGAGGC ACAAGGGGAC TGGACTGTCT CCCTTGAACA TATTTTAGCC
    TCACTTCTGA CTGAACAGTC ACTAGTCAAC TTTTTTGAAA AGCCACTGGA CATTAAATCC
    AAACTTGAAA ACGCCAAGCT CAATCAGTAC AGCCTCAAGA CTTGTGAAAT GAGCCGCCAG
    TCACAGCCTG AGCTCAAGCT GAGCAGCCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854558.1
    CDS16..3348
    Translation

    Target ORF information:

    RefSeq Version XM_024998790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGAGTTA TCTTCAGCTT CTTTATAGAA GGTCGCCTGG TGGGTGCCCT GGACGCGGTG 
    CTGGATTCCA ACGCCCGGGT TGCTCCGTTC CGAATTCTGC TCCAGGTGCC CGGCTCCCAG
    GCGTATTCTC CCATAGCATG TGGTGCCACG TTAGAGGAAA TAAACCAGCA CTGGGACTGG
    CTGGAGCAAA ATCTCCTCCA CACCTTGTCT GTCTTTGATA ATAAAGATGA CATTGCCAGT
    TTTGTCAAAG GGAAGGTGAA GGCTCTGATT GCCGAGGAGA CGAGCAGCAG GCTGGCCGAG
    CAGGAGGAGG AGCCTGAGAA ATTCCGCGAG GCCTTGGTGA AGTTCGAGAC CAGGTTCAAC
    TTCCCGGAAG CTGAGAAGCT GATCACATAT TACTCATGCT GCTGCTGGAA GGGCCGGGTG
    CCCCGCCAAG GCTGGCTGTA CCTGAGCATC AACCACCTCT GCTTCTATTC CTTCTTCTTG
    GGCAAGGAGC TTAAACTTGT CATCCCTTGG GTTGACGTCC AGAGGTTGGA GAGGACATCC
    AATGTCTTTC TGACGGATAC CATACGCATC ACCACCCAGA ACAAGGAGCG GGACTTCTCC
    ATGTTTCTAA ACCTGGACGA GGTGTTCAAG ATCATGGAGC AGCTGGCCGA CGTGACGCTG
    CGGAGGCTGC TGGACAATGA GGGCTTCGAC CTGGACCCGG GTCTGCAGGA GCCCAGCCAG
    ATCACCAAGA GGGACCTGGA AGCTAGGGCA CAGAATGAGT TCTTCCGGGC CTTCTTCAGG
    CTGCCAAGGC GGGAGAAGCT GCACGCCGTC CTGGACTGTT CCCTCTGGAC CCCATTCAGC
    CGCTGTCACA CCGCCGGGCA GATGTTCACC TCCGACAGTT ACATCTGCTT TGCCAGCAAG
    GAGGCCGGCT GCTGCAAAGT CATCCTGCCG CTCAGAGAGG TTGTGAGCAT CGAGAAAATG
    GAGGACACGA GTCTGCTGCC AAACCCCATC ATCGTCAGCA TCCGGAGCAA GATGGCCTTC
    CAGTTCATTG AGCTCAGAGA TCGTGACAGC CTGGTGGAGG GTCTGCTGGC GAGACTGAAG
    CTGGTGCATG CCGACCACCC TGTGCACTAT GACACCACCC TGGATGAGGA GCTGACCTCA
    CCCGTGTTTC ATTCAACAAG CTTGTGCAGT GGCAGCCACA GGTTTGGGGG TCTTGAGATG
    GTGTCTTCGC AAAATAGCGA GGAAAGGGAG AAAGAAACGA GCCCACGGAT GCACCCCGAG
    GCTTTCAGGC AGTCGGGCAG CCAGAGCCCG GACTCGGGAT TGTCCAGGGA ACAGATCAAG
    GTGAGCCTCT GGAAAGACCA CTTTGTGGAG TACGGCAGAA CAGTGTGCAT GTTCCGCACA
    GAGAAGATCC GGAAGCTGGT GGCCATGGGG ATCCCCGAGT CCCTGCGCGG CAGACTCTGG
    CTTCTTTTCT CAGATGCCGT CACGGACCTT GCCGCGCATC CGGGTTACTA CGGCAACCTG
    GTGGAGGAGT CCATGGGAAA GTGTTGCCTG GTGACGGAGG AGATAGAGCG GGACCTGCAC
    CGTTCCTTGC CGGAGCACCC CGCCTTCCAA AACGAAACGG GCATCGCAGC GTTGAGGAGA
    GTCCTGACGG CCTATGCCCA CAGGAACCCC AAGATTGGGT ACTGCCAGTC CATGAACATC
    CTGACCTCCG TTCTGCTGCT GTATGCTAAG GAGGAGGAAG CCTTCTGGCT GCTGGTGGCT
    GTGTGTGAGC GGATGCTGCC CGACTACTTC AACCACCGAG TCATTGGGGC CCAGGTGGAC
    CAGTCCGTCT TCGAGGAGCT CATCAAGGAG CAGCTCCCTG AGCTGGCGGA GCACATGCAC
    GACCTCTCGG CCCTGGCGTC CATCTCCCTG TCCTGGTTCC TCACGCTCTT CCTCAGCATC
    ATGCCCCTGG AGAGTGCCGT GAACGTGGTC GACTGCTTCT TCTACGATGG CATCAAGGCC
    ATCTTCCAGC TGGGACTGGC TGTGTTGGAG GCCACTGCCG AGGGCCTGTG CAGCAGCAAG
    GATGACAGCC AGGCCTTCAT GGTCCTCAGC AGGTTTCTGG ACCACGTCAA GAACGAGGAC
    AGCCCGGGAC CCCCGATTGG CAGCCACCAC GCCTTCTTCT CTGACGACCA GGAGCCGTGT
    CCTGTGACCG ACATCGCAGA CCTGATCCGG GACTCCTACG AGAAATTTGG GGACCAGTCT
    GTGGCTCAGA TCGAGCACAT GCGCTGCAGG CACAGGATCC GAGTCCTCCA GGGCCACGAG
    GACACCACGA AGCAGAACGT GCTCCGCGTC GTTATCCCAG AAGTCTCTGT CCTTCCTGAA
    GACCTGGAGG AGCTCTACGA TTTATTCAAG AGAGAACACA TGATGAGCTG CTACTGGGAG
    CAGCCTCGGC CCATGGCCCC ACGCCACGAC CCCAGCAGGC CCTACGCAGA GCAGTACCGC
    ATAGATGCCC AGCAGTTTGC ACGCCTGTTC CAGCTCGTCT CGCCGTGGAC CTGCGGGGCC
    CACACGGAAA TCCTCGCAGA AAGGACTTTC AGGCTTCTGG ACGAAAACAT GGACCACCTT
    ATCGAATTCA AAGCATTCGT GAGCTGCCTC GATATTATGT ATAATGGAGA AATGAATGAG
    AAAATTAAAC TGTTATATCG GCTTCATATC CCTCCAGCAC TTACTGAAAA TGACCGAGAC
    AGCCAGTCAC CACTGAAGAA CCCTCTGTTG TCGACGTCAA GACCCCTGGT TTTCGGCAAG
    TCCAATGGTG ATACAATTGA TTATCAGAAA CAGCTGAAGC AGATGATTAA AGATTTGGCC
    AAAGAGAAAG ACAAAACTGA GAAAGAGTTG CCCAAAATGA GCCAGAGAGA GTTTATTCAG
    TTCTGTAAGA CTTTGTACAG TATGTTCCAC GAAGACCCGG AAGAGAATGA CCTGTACCAA
    GCCATCGCCA CAGTAACGAC GCTGCTGCTG CAGATTGGGG AGGTGGGGCA GCGCGGCAGC
    AGCTCTGGGA GCGGCTCCCA GGAAGGCGGG GAGGAGCTGC AGGCCTCGGC TCCTTCCCCC
    TCTTCTGAGC AGGACTTGGT GTTTGCCGAG ACTGACACAG GGCAGAGTCC TCAGGCGTCC
    CAGGCATTTC CTGAGGAGGC ACAAGGGGAC TGGACTGTCT CCCTTGAACA TATTTTAGCC
    TCACTTCTGA CTGAACAGTC ACTAGTCAAC TTTTTTGAAA AGCCACTGGA CATTAAATCC
    AAACTTGAAA ACGCCAAGCT CAATCAGTAC AGCCTCAAGA CTTGTGAAAT GAGCCGCCAG
    TCACAGCCTG AGCTCAAGCT GAGCAGCCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220417
    Clone ID Related Accession (Same CDS sequence) XM_010809739.3
    Accession Version XM_010809739.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CCGAGCAGGA GGAGGAGCCT
    GAGAAATTCC GCGAGGCCTT GGTGAAGTTC GAGACCAGGT TCAACTTCCC GGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAA
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC TGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808041.2
    CDS137..3454
    Translation

    Target ORF information:

    RefSeq Version XM_010809739.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809739.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CCGAGCAGGA GGAGGAGCCT
    GAGAAATTCC GCGAGGCCTT GGTGAAGTTC GAGACCAGGT TCAACTTCCC GGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAA
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC TGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220418
    Clone ID Related Accession (Same CDS sequence) XM_024998791.1
    Accession Version XM_024998791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGTT
    AAACTTGTCA TCCCTTGGGT TGACGTCCAG AGGTTGGAGA GGACATCCAA TGTCTTTCTG
    ACGGATACCA TACGCATCAC CACCCAGAAC AAGGAGCGGG ACTTCTCCAT GTTTCTAAAC
    CTGGACGAGG TGTTCAAGAT CATGGAGCAG CTGGCCGACG TGACGCTGCG GAGGCTGCTG
    GACAATGAGG GCTTCGACCT GGACCCGGGT CTGCAGGAGC CCAGCCAGAT CACCAAGAGG
    GACCTGGAAG CTAGGGCACA GAATGAGTTC TTCCGGGCCT TCTTCAGGCT GCCAAGGCGG
    GAGAAGCTGC ACGCCGTCCT GGACTGTTCC CTCTGGACCC CATTCAGCCG CTGTCACACC
    GCCGGGCAGA TGTTCACCTC CGACAGTTAC ATCTGCTTTG CCAGCAAGGA GGCCGGCTGC
    TGCAAAGTCA TCCTGCCGCT CAGAGAGGTT GTGAGCATCG AGAAAATGGA GGACACGAGT
    CTGCTGCCAA ACCCCATCAT CGTCAGCATC CGGAGCAAGA TGGCCTTCCA GTTCATTGAG
    CTCAGAGATC GTGACAGCCT GGTGGAGGGT CTGCTGGCGA GACTGAAGCT GGTGCATGCC
    GACCACCCTG TGCACTATGA CACCACCCTG GATGAGGAGC TGACCTCACC CGTGTTTCAT
    TCAACAAGCT TGTGCAGTGG CAGCCACAGG TTTGGGGGTC TTGAGATGGT GTCTTCGCAA
    AATAGCGAGG AAAGGGAGAA AGAAACGAGC CCACGGATGC ACCCCGAGGC TTTCAGGCAG
    TCGGGCAGCC AGAGCCCGGA CTCGGGATTG TCCAGGGAAC AGATCAAGGT GAGCCTCTGG
    AAAGACCACT TTGTGGAGTA CGGCAGAACA GTGTGCATGT TCCGCACAGA GAAGATCCGG
    AAGCTGGTGG CCATGGGGAT CCCCGAGTCC CTGCGCGGCA GACTCTGGCT TCTTTTCTCA
    GATGCCGTCA CGGACCTTGC CGCGCATCCG GGTTACTACG GCAACCTGGT GGAGGAGTCC
    ATGGGAAAGT GTTGCCTGGT GACGGAGGAG ATAGAGCGGG ACCTGCACCG TTCCTTGCCG
    GAGCACCCCG CCTTCCAAAA CGAAACGGGC ATCGCAGCGT TGAGGAGAGT CCTGACGGCC
    TATGCCCACA GGAACCCCAA GATTGGGTAC TGCCAGTCCA TGAACATCCT GACCTCCGTT
    CTGCTGCTGT ATGCTAAGGA GGAGGAAGCC TTCTGGCTGC TGGTGGCTGT GTGTGAGCGG
    ATGCTGCCCG ACTACTTCAA CCACCGAGTC ATTGGGGCCC AGGTGGACCA GTCCGTCTTC
    GAGGAGCTCA TCAAGGAGCA GCTCCCTGAG CTGGCGGAGC ACATGCACGA CCTCTCGGCC
    CTGGCGTCCA TCTCCCTGTC CTGGTTCCTC ACGCTCTTCC TCAGCATCAT GCCCCTGGAG
    AGTGCCGTGA ACGTGGTCGA CTGCTTCTTC TACGATGGCA TCAAGGCCAT CTTCCAGCTG
    GGACTGGCTG TGTTGGAGGC CACTGCCGAG GGCCTGTGCA GCAGCAAGGA TGACAGCCAG
    GCCTTCATGG TCCTCAGCAG GTTTCTGGAC CACGTCAAGA ACGAGGACAG CCCGGGACCC
    CCGATTGGCA GCCACCACGC CTTCTTCTCT GACGACCAGG AGCCGTGTCC TGTGACCGAC
    ATCGCAGACC TGATCCGGGA CTCCTACGAG AAATTTGGGG ACCAGTCTGT GGCTCAGATC
    GAGCACATGC GCTGCAGGCA CAGGATCCGA GTCCTCCAGG GCCACGAGGA CACCACGAAG
    CAGAACGTGC TCCGCGTCGT TATCCCAGAA GTCTCTGTCC TTCCTGAAGA CCTGGAGGAG
    CTCTACGATT TATTCAAGAG AGAACACATG ATGAGCTGCT ACTGGGAGCA GCCTCGGCCC
    ATGGCCCCAC GCCACGACCC CAGCAGGCCC TACGCAGAGC AGTACCGCAT AGATGCCCAG
    CAGTTTGCAC GCCTGTTCCA GCTCGTCTCG CCGTGGACCT GCGGGGCCCA CACGGAAATC
    CTCGCAGAAA GGACTTTCAG GCTTCTGGAC GAAAACATGG ACCACCTTAT CGAATTCAAA
    GCATTCGTGA GCTGCCTCGA TATTATGTAT AATGGAGAAA TGAATGAGAA AATTAAACTG
    TTATATCGGC TTCATATCCC TCCAGCACTT ACTGAAAATG ACCGAGACAG CCAGTCACCA
    CTGAAGAACC CTCTGTTGTC GACGTCAAGA CCCCTGGTTT TCGGCAAGTC CAATGGTGAT
    ACAATTGATT ATCAGAAACA GCTGAAGCAG ATGATTAAAG ATTTGGCCAA AGAGAAAGAC
    AAAACTGAGA AAGAGTTGCC CAAAATGAGC CAGAGAGAGT TTATTCAGTT CTGTAAGACT
    TTGTACAGTA TGTTCCACGA AGACCCGGAA GAGAATGACC TGTACCAAGC CATCGCCACA
    GTAACGACGC TGCTGCTGCA GATTGGGGAG GTGGGGCAGC GCGGCAGCAG CTCTGGGAGC
    GGCTCCCAGG AAGGCGGGGA GGAGCTGCAG GCCTCGGCTC CTTCCCCCTC TTCTGAGCAG
    GACTTGGTGT TTGCCGAGAC TGACACAGGG CAGAGTCCTC AGGCGTCCCA GGCATTTCCT
    GAGGAGGCAC AAGGGGACTG GACTGTCTCC CTTGAACATA TTTTAGCCTC ACTTCTGACT
    GAACAGTCAC TAGTCAACTT TTTTGAAAAG CCACTGGACA TTAAATCCAA ACTTGAAAAC
    GCCAAGCTCA ATCAGTACAG CCTCAAGACT TGTGAAATGA GCCGCCAGTC ACAGCCTGAG
    CTCAAGCTGA GCAGCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854559.1
    CDS113..3193
    Translation

    Target ORF information:

    RefSeq Version XM_024998791.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGTT
    AAACTTGTCA TCCCTTGGGT TGACGTCCAG AGGTTGGAGA GGACATCCAA TGTCTTTCTG
    ACGGATACCA TACGCATCAC CACCCAGAAC AAGGAGCGGG ACTTCTCCAT GTTTCTAAAC
    CTGGACGAGG TGTTCAAGAT CATGGAGCAG CTGGCCGACG TGACGCTGCG GAGGCTGCTG
    GACAATGAGG GCTTCGACCT GGACCCGGGT CTGCAGGAGC CCAGCCAGAT CACCAAGAGG
    GACCTGGAAG CTAGGGCACA GAATGAGTTC TTCCGGGCCT TCTTCAGGCT GCCAAGGCGG
    GAGAAGCTGC ACGCCGTCCT GGACTGTTCC CTCTGGACCC CATTCAGCCG CTGTCACACC
    GCCGGGCAGA TGTTCACCTC CGACAGTTAC ATCTGCTTTG CCAGCAAGGA GGCCGGCTGC
    TGCAAAGTCA TCCTGCCGCT CAGAGAGGTT GTGAGCATCG AGAAAATGGA GGACACGAGT
    CTGCTGCCAA ACCCCATCAT CGTCAGCATC CGGAGCAAGA TGGCCTTCCA GTTCATTGAG
    CTCAGAGATC GTGACAGCCT GGTGGAGGGT CTGCTGGCGA GACTGAAGCT GGTGCATGCC
    GACCACCCTG TGCACTATGA CACCACCCTG GATGAGGAGC TGACCTCACC CGTGTTTCAT
    TCAACAAGCT TGTGCAGTGG CAGCCACAGG TTTGGGGGTC TTGAGATGGT GTCTTCGCAA
    AATAGCGAGG AAAGGGAGAA AGAAACGAGC CCACGGATGC ACCCCGAGGC TTTCAGGCAG
    TCGGGCAGCC AGAGCCCGGA CTCGGGATTG TCCAGGGAAC AGATCAAGGT GAGCCTCTGG
    AAAGACCACT TTGTGGAGTA CGGCAGAACA GTGTGCATGT TCCGCACAGA GAAGATCCGG
    AAGCTGGTGG CCATGGGGAT CCCCGAGTCC CTGCGCGGCA GACTCTGGCT TCTTTTCTCA
    GATGCCGTCA CGGACCTTGC CGCGCATCCG GGTTACTACG GCAACCTGGT GGAGGAGTCC
    ATGGGAAAGT GTTGCCTGGT GACGGAGGAG ATAGAGCGGG ACCTGCACCG TTCCTTGCCG
    GAGCACCCCG CCTTCCAAAA CGAAACGGGC ATCGCAGCGT TGAGGAGAGT CCTGACGGCC
    TATGCCCACA GGAACCCCAA GATTGGGTAC TGCCAGTCCA TGAACATCCT GACCTCCGTT
    CTGCTGCTGT ATGCTAAGGA GGAGGAAGCC TTCTGGCTGC TGGTGGCTGT GTGTGAGCGG
    ATGCTGCCCG ACTACTTCAA CCACCGAGTC ATTGGGGCCC AGGTGGACCA GTCCGTCTTC
    GAGGAGCTCA TCAAGGAGCA GCTCCCTGAG CTGGCGGAGC ACATGCACGA CCTCTCGGCC
    CTGGCGTCCA TCTCCCTGTC CTGGTTCCTC ACGCTCTTCC TCAGCATCAT GCCCCTGGAG
    AGTGCCGTGA ACGTGGTCGA CTGCTTCTTC TACGATGGCA TCAAGGCCAT CTTCCAGCTG
    GGACTGGCTG TGTTGGAGGC CACTGCCGAG GGCCTGTGCA GCAGCAAGGA TGACAGCCAG
    GCCTTCATGG TCCTCAGCAG GTTTCTGGAC CACGTCAAGA ACGAGGACAG CCCGGGACCC
    CCGATTGGCA GCCACCACGC CTTCTTCTCT GACGACCAGG AGCCGTGTCC TGTGACCGAC
    ATCGCAGACC TGATCCGGGA CTCCTACGAG AAATTTGGGG ACCAGTCTGT GGCTCAGATC
    GAGCACATGC GCTGCAGGCA CAGGATCCGA GTCCTCCAGG GCCACGAGGA CACCACGAAG
    CAGAACGTGC TCCGCGTCGT TATCCCAGAA GTCTCTGTCC TTCCTGAAGA CCTGGAGGAG
    CTCTACGATT TATTCAAGAG AGAACACATG ATGAGCTGCT ACTGGGAGCA GCCTCGGCCC
    ATGGCCCCAC GCCACGACCC CAGCAGGCCC TACGCAGAGC AGTACCGCAT AGATGCCCAG
    CAGTTTGCAC GCCTGTTCCA GCTCGTCTCG CCGTGGACCT GCGGGGCCCA CACGGAAATC
    CTCGCAGAAA GGACTTTCAG GCTTCTGGAC GAAAACATGG ACCACCTTAT CGAATTCAAA
    GCATTCGTGA GCTGCCTCGA TATTATGTAT AATGGAGAAA TGAATGAGAA AATTAAACTG
    TTATATCGGC TTCATATCCC TCCAGCACTT ACTGAAAATG ACCGAGACAG CCAGTCACCA
    CTGAAGAACC CTCTGTTGTC GACGTCAAGA CCCCTGGTTT TCGGCAAGTC CAATGGTGAT
    ACAATTGATT ATCAGAAACA GCTGAAGCAG ATGATTAAAG ATTTGGCCAA AGAGAAAGAC
    AAAACTGAGA AAGAGTTGCC CAAAATGAGC CAGAGAGAGT TTATTCAGTT CTGTAAGACT
    TTGTACAGTA TGTTCCACGA AGACCCGGAA GAGAATGACC TGTACCAAGC CATCGCCACA
    GTAACGACGC TGCTGCTGCA GATTGGGGAG GTGGGGCAGC GCGGCAGCAG CTCTGGGAGC
    GGCTCCCAGG AAGGCGGGGA GGAGCTGCAG GCCTCGGCTC CTTCCCCCTC TTCTGAGCAG
    GACTTGGTGT TTGCCGAGAC TGACACAGGG CAGAGTCCTC AGGCGTCCCA GGCATTTCCT
    GAGGAGGCAC AAGGGGACTG GACTGTCTCC CTTGAACATA TTTTAGCCTC ACTTCTGACT
    GAACAGTCAC TAGTCAACTT TTTTGAAAAG CCACTGGACA TTAAATCCAA ACTTGAAAAC
    GCCAAGCTCA ATCAGTACAG CCTCAAGACT TGTGAAATGA GCCGCCAGTC ACAGCCTGAG
    CTCAAGCTGA GCAGCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51323
    Clone ID Related Accession (Same CDS sequence) XM_010809739.2
    Accession Version XM_010809739.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CTGAGCAGGA GGAGGAGCCT
    GAGAAGTTCC GCGAGGCCTT GGTGAAGTTC GAGGCCAGGT TCAACTTCCC AGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAC
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC CGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808041.1
    CDS149..3466
    Translation

    Target ORF information:

    RefSeq Version XM_010809739.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTG CCACGTTAGA GGAAATAAAC CAGCACTGGG ACTGGCTGGA GCAAAATCTC
    CTCCACACCT TGTCTGTCTT TGATAATAAA GATGACATTG CCAGTTTTGT CAAAGGGAAG
    GTGAAGGCTC TGATTGCCGA GGAGACGAGC AGCAGGCTGG CTGAGCAGGA GGAGGAGCCT
    GAGAAGTTCC GCGAGGCCTT GGTGAAGTTC GAGGCCAGGT TCAACTTCCC AGAAGCTGAG
    AAGCTGATCA CATATTACTC ATGCTGCTGC TGGAAGGGCC GGGTGCCCCG CCAAGGCTGG
    CTGTACCTGA GCATCAACCA CCTCTGCTTC TATTCCTTCT TCTTGGGCAA GGAGCTTAAA
    CTTGTCATCC CTTGGGTTGA CGTCCAGAGG TTGGAGAGGA CATCCAATGT CTTTCTGACG
    GATACCATAC GCATCACCAC CCAGAACAAG GAGCGGGACT TCTCCATGTT TCTAAACCTG
    GACGAGGTGT TCAAGATCAT GGAGCAGCTG GCCGACGTGA CGCTGCGGAG GCTGCTGGAC
    AATGAGGGCT TCGACCTGGA CCCGGGTCTG CAGGAGCCCA GCCAGATCAC CAAGAGGGAC
    CTGGAAGCTA GGGCACAGAA TGAGTTCTTC CGGGCCTTCT TCAGGCTGCC AAGGCGGGAG
    AAGCTGCACG CCGTCCTGGA CTGTTCCCTC TGGACCCCAT TCAGCCGCTG TCACACCGCC
    GGGCAGATGT TCACCTCCGA CAGTTACATC TGCTTTGCCA GCAAGGAGGC CGGCTGCTGC
    AAAGTCATCC TGCCGCTCAG AGAGGTTGTG AGCATCGAGA AAATGGAGGA CACGAGTCTG
    CTGCCAAACC CCATCATCGT CAGCATCCGG AGCAAGATGG CCTTCCAGTT CATTGAGCTC
    AGAGATCGTG ACAGCCTGGT GGAGGGTCTG CTGGCGAGAC TGAAGCTGGT GCATGCCGAC
    CACCCTGTGC ACTATGACAC CACCCTGGAT GAGGAGCTGA CCTCACCCGT GTTTCATTCA
    ACAAGCTTGT GCAGTGGCAG CCACAGGTTT GGGGGTCTTG AGATGGTGTC TTCGCAAAAT
    AGCGAGGAAA GGGAGAAAGA AACGAGCCCA CGGATGCACC CCGAGGCTTT CAGGCAGTCG
    GGCAGCCAGA GCCCGGACTC GGGATTGTCC AGGGAACAGA TCAAGGTGAG CCTCTGGAAC
    GACCACTTTG TGGAGTACGG CAGAACAGTG TGCATGTTCC GCACAGAGAA GATCCGGAAG
    CTGGTGGCCA TGGGGATCCC CGAGTCCCTG CGCGGCAGAC TCTGGCTTCT TTTCTCAGAT
    GCCGTCACGG ACCTTGCCGC GCATCCGGGT TACTACGGCA ACCTGGTGGA GGAGTCCATG
    GGAAAGTGTT GCCTGGTGAC GGAGGAGATA GAGCGGGACC TGCACCGTTC CTTGCCGGAG
    CACCCCGCCT TCCAAAACGA AACGGGCATC GCAGCGTTGA GGAGAGTCCT GACGGCCTAT
    GCCCACAGGA ACCCCAAGAT TGGGTACTGC CAGTCCATGA ACATCCTGAC CTCCGTTCTG
    CTGCTGTATG CTAAGGAGGA GGAAGCCTTC TGGCTGCTGG TGGCTGTGTG TGAGCGGATG
    CTGCCCGACT ACTTCAACCA CCGAGTCATT GGGGCCCAGG TGGACCAGTC CGTCTTCGAG
    GAGCTCATCA AGGAGCAGCT CCCTGAGCTG GCGGAGCACA TGCACGACCT CTCGGCCCTG
    GCGTCCATCT CCCTGTCCTG GTTCCTCACG CTCTTCCTCA GCATCATGCC CCTGGAGAGT
    GCCGTGAACG TGGTCGACTG CTTCTTCTAC GATGGCATCA AGGCCATCTT CCAGCTGGGA
    CTGGCTGTGT TGGAGGCCAC TGCCGAGGGC CTGTGCAGCA GCAAGGATGA CAGCCAGGCC
    TTCATGGTCC TCAGCAGGTT TCTGGACCAC GTCAAGAACG AGGACAGCCC GGGACCCCCG
    ATTGGCAGCC ACCACGCCTT CTTCTCTGAC GACCAGGAGC CGTGTCCTGT GACCGACATC
    GCAGACCTGA TCCGGGACTC CTACGAGAAA TTTGGGGACC AGTCTGTGGC TCAGATCGAG
    CACATGCGCT GCAGGCACAG GATCCGAGTC CTCCAGGGCC ACGAGGACAC CACGAAGCAG
    AACGTGCTCC GCGTCGTTAT CCCAGAAGTC TCTGTCCTTC CTGAAGACCT GGAGGAGCTC
    TACGATTTAT TCAAGAGAGA ACACATGATG AGCTGCTACT GGGAGCAGCC TCGGCCCATG
    GCCCCACGCC ACGACCCCAG CAGGCCCTAC GCAGAGCAGT ACCGCATAGA TGCCCAGCAG
    TTTGCACGCC TGTTCCAGCT CGTCTCGCCG TGGACCTGCG GGGCCCACAC GGAAATCCTC
    GCAGAAAGGA CTTTCAGGCT TCTGGACGAA AACATGGACC ACCTTATCGA ATTCAAAGCA
    TTCGTGAGCT GCCTCGATAT TATGTATAAT GGAGAAATGA ATGAGAAAAT TAAACTGTTA
    TATCGGCTTC ATATCCCTCC AGCACTTACT GAAAATGACC GAGACAGCCA GTCACCACTG
    AAGAACCCTC TGTTGTCGAC GTCAAGACCC CTGGTTTTCG GCAAGTCCAA TGGTGATACA
    ATTGATTATC AGAAACAGCT GAAGCAGATG ATTAAAGATT TGGCCAAAGA GAAAGACAAA
    ACTGAGAAAG AGTTGCCCAA AATGAGCCAG AGAGAGTTTA TTCAGTTCTG TAAGACTTTG
    TACAGTATGT TCCACGAAGA CCCGGAAGAG AATGACCTGT ACCAAGCCAT CGCCACAGTA
    ACGACGCTGC TGCTGCAGAT TGGGGAGGTG GGGCAGCGCG GCAGCAGCTC TGGGAGCGGC
    TCCCAGGAAG GCGGGGAGGA GCTGCAGGCC TCGGCTCCTT CCCCCTCTTC TGAGCAGGAC
    TTGGTGTTTG CCGAGACTGA CACAGGGCAG AGTCCTCAGG CGTCCCAGGC ATTTCCTGAG
    GAGGCACAAG GGGACTGGAC CGTCTCCCTT GAACATATTT TAGCCTCACT TCTGACTGAA
    CAGTCACTAG TCAACTTTTT TGAAAAGCCA CTGGACATTA AATCCAAACT TGAAAACGCC
    AAGCTCAATC AGTACAGCCT CAAGACTTGT GAAATGAGCC GCCAGTCACA GCCTGAGCTC
    AAGCTGAGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01676
    Clone ID Related Accession (Same CDS sequence) NM_001206142.1
    Accession Version NM_001206142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product TBC1 domain family member 8
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03010393.1, AAFC03041582.1, AAFC03041581.1, AAFC03050127.1 and AAFC03050128.1. On Apr 20, 2011 this sequence version replaced XM_864858.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCTGAGCAGG AGGAGGAGCC TGAGAAGTTC
    CGCGAGGCCT TGGTGAAGTT CGAGGCCAGG TTCAACTTCC CAGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA CGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TCGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CCGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193071.1
    CDS135..3563
    Translation

    Target ORF information:

    RefSeq Version NM_001206142.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus TBC1 domain family, member 8 (with GRAM domain) (TBC1D8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCTGAGCAGG AGGAGGAGCC TGAGAAGTTC
    CGCGAGGCCT TGGTGAAGTT CGAGGCCAGG TTCAACTTCC CAGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA CGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TCGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CCGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216378
    Clone ID Related Accession (Same CDS sequence) NM_001206142.2
    Accession Version NM_001206142.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02030203.1, DAAA02030202.1 and DAAA02030201.1. On Jun 30, 2017 this sequence version replaced NM_001206142.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCTGAGCAGG AGGAGGAGCC TGAGAAGTTC
    CGCGAGGCCT TGGTGAAGTT CGAGGCCAGG TTCAACTTCC CAGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA CGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TTGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CCGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193071.1
    CDS135..3563
    Translation

    Target ORF information:

    RefSeq Version NM_001206142.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GCCACGTTAG AGGAAATAAA CCAGCACTGG GACTGGCTGG AGCAAAATCT CCTCCACACC
    TTGTCTGTCT TTGATAATAA AGATGACATT GCCAGTTTTG TCAAAGGGAA GGTGAAGGCT
    CTGATTGCCG AGGAGACGAG CAGCAGGCTG GCTGAGCAGG AGGAGGAGCC TGAGAAGTTC
    CGCGAGGCCT TGGTGAAGTT CGAGGCCAGG TTCAACTTCC CAGAAGCTGA GAAGCTGATC
    ACATATTACT CATGCTGCTG CTGGAAGGGC CGGGTGCCCC GCCAAGGCTG GCTGTACCTG
    AGCATCAACC ACCTCTGCTT CTATTCCTTC TTCTTGGGCA AGGAGCTTAA ACTTGTCATC
    CCTTGGGTTG ACGTCCAGAG GTTGGAGAGG ACATCCAATG TCTTTCTGAC GGATACCATA
    CGCATCACCA CCCAGAACAA GGAGCGGGAC TTCTCCATGT TTCTAAACCT GGACGAGGTG
    TTCAAGATCA TGGAGCAGCT GGCCGACGTG ACGCTGCGGA GGCTGCTGGA CAATGAGGGC
    TTCGACCTGG ACCCGGGTCT GCAGGAGCCC AGCCAGATCA CCAAGAGGGA CCTGGAAGCT
    AGGGCACAGA ATGAGTTCTT CCGGGCCTTC TTCAGGCTGC CAAGGCGGGA GAAGCTGCAC
    GCCGTCCTGG ACTGTTCCCT CTGGACCCCA TTCAGCCGCT GTCACACCGC CGGGCAGATG
    TTCACCTCCG ACAGTTACAT CTGCTTTGCC AGCAAGGAGG CCGGCTGCTG CAAAGTCATC
    CTGCCGCTCA GAGAGGTTGT GAGCATCGAG AAAATGGAGG ACACGAGTCT GCTGCCAAAC
    CCCATCATCG TCAGCATCCG GAGCAAGATG GCCTTCCAGT TCATTGAGCT CAGAGATCGT
    GACAGCCTGG TGGAGGGTCT GCTGGCGAGA CTGAAGCTGG TGCATGCCGA CCACCCTGTG
    CACTATGACA CCACCCTGGA TGAGGAGCTG ACCTCACCCG TGTTTCATTC AACAAGCTTG
    TGCAGTGGCA GCCACAGGTT TGGGGGTCTT GAGATGGTGT CTTCGCAAAA TAGCGAGGAA
    AGGGAGAAAG AAACGAGCCC ACGGATGCAC CCCGAGGCTT TCAGGCAGTC GGGCAGCCAG
    AGCCCGGACT CGGGATTGTC CAGGGAACAG ATCAAGGTGA GCCTCTGGAA CGACCACTTT
    GTGGAGTACG GCAGAACAGT GTGCATGTTC CGCACAGAGA AGATCCGGAA GCTGGTGGCC
    ATGGGGATCC CCGAGTCCCT GCGCGGCAGA CTCTGGCTTC TTTTCTCAGA TGCCGTCACG
    GACCTTGCCG CGCATCCGGG TTACTACGGC AACCTGGTGG AGGAGTCCAT GGGAAAGTGT
    TGCCTGGTGA CGGAGGAGAT AGAGCGGGAC CTGCACCGTT CCTTGCCGGA GCACCCCGCC
    TTCCAAAACG AAACGGGCAT CGCAGCGTTG AGGAGAGTCC TGACGGCCTA TGCCCACAGG
    AACCCCAAGA TTGGGTACTG CCAGTCCATG AACATCCTGA CCTCCGTTCT GCTGCTGTAT
    GCTAAGGAGG AGGAAGCCTT CTGGCTGCTG GTGGCTGTGT GTGAGCGGAT GCTGCCCGAC
    TACTTCAACC ACCGAGTCAT TGGGGCCCAG GTGGACCAGT CCGTCTTCGA GGAGCTCATC
    AAGGAGCAGC TCCCTGAGCT GGCGGAGCAC ATGCACGACC TCTCGGCCCT GGCGTCCATC
    TCCCTGTCCT GGTTCCTCAC GCTCTTCCTC AGCATCATGC CCCTGGAGAG TGCCGTGAAC
    GTGGTCGACT GCTTCTTCTA CGATGGCATC AAGGCCATCT TCCAGCTGGG ACTGGCTGTG
    TTGGAGGCCA CTGCCGAGGG CCTGTGCAGC AGCAAGGATG ACAGCCAGGC CTTCATGGTC
    CTCAGCAGGT TTCTGGACCA CGTCAAGAAC GAGGACAGCC CGGGACCCCC GATTGGCAGC
    CACCACGCCT TCTTCTCTGA CGACCAGGAG CCGTGTCCTG TGACCGACAT CGCAGACCTG
    ATCCGGGACT CCTACGAGAA ATTTGGGGAC CAGTCTGTGG CTCAGATCGA GCACATGCGC
    TGCAGGCACA GGATCCGAGT CCTCCAGGGC CACGAGGACA CCACGAAGCA GAACGTGCTC
    CGCGTCGTTA TCCCAGAAGT CTCTGTCCTT CCTGAAGACC TGGAGGAGCT CTACGATTTA
    TTCAAGAGAG AACACATGAT GAGCTGCTAC TGGGAGCAGC CTCGGCCCAT GGCCCCACGC
    CACGACCCCA GCAGGCCCTA CGCAGAGCAG TACCGCATAG ATGCCCAGCA GTTTGCACGC
    CTGTTCCAGC TCGTCTCGCC GTGGACCTGC GGGGCCCACA CGGAAATCCT CGCAGAAAGG
    ACTTTCAGGC TTCTGGACGA AAACATGGAC CACCTTATCG AATTCAAAGC ATTCGTGAGC
    TGCCTCGATA TTATGTATAA TGGAGAAATG AATGAGAAAA TTAAACTGTT ATATCGGCTT
    CATATCCCTC CAGCACTTAC TGAAAATGAC CGAGACAGCC AGTCACCACT GAAGAACCCT
    CTGTTGTCGA CGTCAAGACC CCTGGTTTTC GGCAAGTCCA ATGGTGATAC AATTGATTAT
    CAGAAACAGC TGAAGCAGAT GATTAAAGAT TTGGCCAAAG AGAAAGACAA AACTGAGAAA
    GAGTTGCCCA AAATGAGCCA GAGAGAGTTT ATTCAGTTCT GTAAGACTTT GTACAGTATG
    TTCCACGAAG ACCCGGAAGA GAATGACCTG TACCAAGCCA TCGCCACAGT AACGACGCTG
    CTGCTGCAGA TTGGGGAGGT GGGGCAGCGC GGCAGCAGCT CTGGGAGCGG CTCCCAGGAA
    GGCGGGGAGG AGCTGCAGGC CTCGGCTCCT TCCCCCTCTT CTGAGCAGGA CTTGGTGTTT
    GCCGAGACTG ACACAGGGCA GAGTCCTCAG GCGTCCCAGG CATTTCCTGA GGAGGCACAA
    GGGGACTGGA CCGTCTCCCT TGAACATATT TTAGCCTCAC TTCTGACTGA ACAGTCACTA
    GTCAACTTTT TTGAAAAGCC ACTGGACATT AAATCCAAAC TTGAAAACGC CAAGCTCAAT
    CAGTACAGCC TCAAGACTTG TGAAATGAGC CGCCAGTCAC AGCCTGAGCT CAAGCTGAGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51322
    Clone ID Related Accession (Same CDS sequence) XM_010809738.2
    Accession Version XM_010809738.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GAGTTACTGA GCGGATCGGA CGTTTACTGG GCCGTGGCCA CGGGTGCCAC GTTAGAGGAA
    ATAAACCAGC ACTGGGACTG GCTGGAGCAA AATCTCCTCC ACACCTTGTC TGTCTTTGAT
    AATAAAGATG ACATTGCCAG TTTTGTCAAA GGGAAGGTGA AGGCTCTGAT TGCCGAGGAG
    ACGAGCAGCA GGCTGGCTGA GCAGGAGGAG GAGCCTGAGA AGTTCCGCGA GGCCTTGGTG
    AAGTTCGAGG CCAGGTTCAA CTTCCCAGAA GCTGAGAAGC TGATCACATA TTACTCATGC
    TGCTGCTGGA AGGGCCGGGT GCCCCGCCAA GGCTGGCTGT ACCTGAGCAT CAACCACCTC
    TGCTTCTATT CCTTCTTCTT GGGCAAGGAG CTTAAACTTG TCATCCCTTG GGTTGACGTC
    CAGAGGTTGG AGAGGACATC CAATGTCTTT CTGACGGATA CCATACGCAT CACCACCCAG
    AACAAGGAGC GGGACTTCTC CATGTTTCTA AACCTGGACG AGGTGTTCAA GATCATGGAG
    CAGCTGGCCG ACGTGACGCT GCGGAGGCTG CTGGACAATG AGGGCTTCGA CCTGGACCCG
    GGTCTGCAGG AGCCCAGCCA GATCACCAAG AGGGACCTGG AAGCTAGGGC ACAGAATGAG
    TTCTTCCGGG CCTTCTTCAG GCTGCCAAGG CGGGAGAAGC TGCACGCCGT CCTGGACTGT
    TCCCTCTGGA CCCCATTCAG CCGCTGTCAC ACCGCCGGGC AGATGTTCAC CTCCGACAGT
    TACATCTGCT TTGCCAGCAA GGAGGCCGGC TGCTGCAAAG TCATCCTGCC GCTCAGAGAG
    GTTGTGAGCA TCGAGAAAAT GGAGGACACG AGTCTGCTGC CAAACCCCAT CATCGTCAGC
    ATCCGGAGCA AGATGGCCTT CCAGTTCATT GAGCTCAGAG ATCGTGACAG CCTGGTGGAG
    GGTCTGCTGG CGAGACTGAA GCTGGTGCAT GCCGACCACC CTGTGCACTA TGACACCACC
    CTGGATGAGG AGCTGACCTC ACCCGTGTTT CATTCAACAA GCTTGTGCAG TGGCAGCCAC
    AGGTTTGGGG GTCTTGAGAT GGTGTCTTCG CAAAATAGCG AGGAAAGGGA GAAAGAAACG
    AGCCCACGGA TGCACCCCGA GGCTTTCAGG CAGTCGGGCA GCCAGAGCCC GGACTCGGGA
    TTGTCCAGGG AACAGATCAA GGTGAGCCTC TGGAACGACC ACTTTGTGGA GTACGGCAGA
    ACAGTGTGCA TGTTCCGCAC AGAGAAGATC CGGAAGCTGG TGGCCATGGG GATCCCCGAG
    TCCCTGCGCG GCAGACTCTG GCTTCTTTTC TCAGATGCCG TCACGGACCT TGCCGCGCAT
    CCGGGTTACT ACGGCAACCT GGTGGAGGAG TCCATGGGAA AGTGTTGCCT GGTGACGGAG
    GAGATAGAGC GGGACCTGCA CCGTTCCTTG CCGGAGCACC CCGCCTTCCA AAACGAAACG
    GGCATCGCAG CGTTGAGGAG AGTCCTGACG GCCTATGCCC ACAGGAACCC CAAGATTGGG
    TACTGCCAGT CCATGAACAT CCTGACCTCC GTTCTGCTGC TGTATGCTAA GGAGGAGGAA
    GCCTTCTGGC TGCTGGTGGC TGTGTGTGAG CGGATGCTGC CCGACTACTT CAACCACCGA
    GTCATTGGGG CCCAGGTGGA CCAGTCCGTC TTCGAGGAGC TCATCAAGGA GCAGCTCCCT
    GAGCTGGCGG AGCACATGCA CGACCTCTCG GCCCTGGCGT CCATCTCCCT GTCCTGGTTC
    CTCACGCTCT TCCTCAGCAT CATGCCCCTG GAGAGTGCCG TGAACGTGGT CGACTGCTTC
    TTCTACGATG GCATCAAGGC CATCTTCCAG CTGGGACTGG CTGTGTTGGA GGCCACTGCC
    GAGGGCCTGT GCAGCAGCAA GGATGACAGC CAGGCCTTCA TGGTCCTCAG CAGGTTTCTG
    GACCACGTCA AGAACGAGGA CAGCCCGGGA CCCCCGATTG GCAGCCACCA CGCCTTCTTC
    TCTGACGACC AGGAGCCGTG TCCTGTGACC GACATCGCAG ACCTGATCCG GGACTCCTAC
    GAGAAATTTG GGGACCAGTC TGTGGCTCAG ATCGAGCACA TGCGCTGCAG GCACAGGATC
    CGAGTCCTCC AGGGCCACGA GGACACCACG AAGCAGAACG TGCTCCGCGT CGTTATCCCA
    GAAGTCTCTG TCCTTCCTGA AGACCTGGAG GAGCTCTACG ATTTATTCAA GAGAGAACAC
    ATGATGAGCT GCTACTGGGA GCAGCCTCGG CCCATGGCCC CACGCCACGA CCCCAGCAGG
    CCCTACGCAG AGCAGTACCG CATAGATGCC CAGCAGTTTG CACGCCTGTT CCAGCTCGTC
    TCGCCGTGGA CCTGCGGGGC CCACACGGAA ATCCTCGCAG AAAGGACTTT CAGGCTTCTG
    GACGAAAACA TGGACCACCT TATCGAATTC AAAGCATTCG TGAGCTGCCT CGATATTATG
    TATAATGGAG AAATGAATGA GAAAATTAAA CTGTTATATC GGCTTCATAT CCCTCCAGCA
    CTTACTGAAA ATGACCGAGA CAGCCAGTCA CCACTGAAGA ACCCTCTGTT GTCGACGTCA
    AGACCCCTGG TTTTCGGCAA GTCCAATGGT GATACAATTG ATTATCAGAA ACAGCTGAAG
    CAGATGATTA AAGATTTGGC CAAAGAGAAA GACAAAACTG AGAAAGAGTT GCCCAAAATG
    AGCCAGAGAG AGTTTATTCA GTTCTGTAAG ACTTTGTACA GTATGTTCCA CGAAGACCCG
    GAAGAGAATG ACCTGTACCA AGCCATCGCC ACAGTAACGA CGCTGCTGCT GCAGATTGGG
    GAGGTGGGGC AGCGCGGCAG CAGCTCTGGG AGCGGCTCCC AGGAAGGCGG GGAGGAGCTG
    CAGGCCTCGG CTCCTTCCCC CTCTTCTGAG CAGGACTTGG TGTTTGCCGA GACTGACACA
    GGGCAGAGTC CTCAGGCGTC CCAGGCATTT CCTGAGGAGG CACAAGGGGA CTGGACCGTC
    TCCCTTGAAC ATATTTTAGC CTCACTTCTG ACTGAACAGT CACTAGTCAA CTTTTTTGAA
    AAGCCACTGG ACATTAAATC CAAACTTGAA AACGCCAAGC TCAATCAGTA CAGCCTCAAG
    ACTTGTGAAA TGAGCCGCCA GTCACAGCCT GAGCTCAAGC TGAGCAGCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808040.1
    CDS149..3622
    Translation

    Target ORF information:

    RefSeq Version XM_010809738.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 8 (TBC1D8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTGGCTCA AGCCCGAGGA GGTGCTGCTG AAGAACGCTC TGAAGCTCTG GGTGACTCAG 
    AAGAGCAACT GCTACTTCAT CCTGCAGCGG CGCCGCGGGC ACGGCGAGGG GGGCGGCCGG
    CTCACGGGTC GCCTGGTGGG TGCCCTGGAC GCGGTGCTGG ATTCCAACGC CCGGGTTGCT
    CCGTTCCGAA TTCTGCTCCA GGTGCCCGGC TCCCAGGCGT ATTCTCCCAT AGCATGTGGT
    GAGTTACTGA GCGGATCGGA CGTTTACTGG GCCGTGGCCA CGGGTGCCAC GTTAGAGGAA
    ATAAACCAGC ACTGGGACTG GCTGGAGCAA AATCTCCTCC ACACCTTGTC TGTCTTTGAT
    AATAAAGATG ACATTGCCAG TTTTGTCAAA GGGAAGGTGA AGGCTCTGAT TGCCGAGGAG
    ACGAGCAGCA GGCTGGCTGA GCAGGAGGAG GAGCCTGAGA AGTTCCGCGA GGCCTTGGTG
    AAGTTCGAGG CCAGGTTCAA CTTCCCAGAA GCTGAGAAGC TGATCACATA TTACTCATGC
    TGCTGCTGGA AGGGCCGGGT GCCCCGCCAA GGCTGGCTGT ACCTGAGCAT CAACCACCTC
    TGCTTCTATT CCTTCTTCTT GGGCAAGGAG CTTAAACTTG TCATCCCTTG GGTTGACGTC
    CAGAGGTTGG AGAGGACATC CAATGTCTTT CTGACGGATA CCATACGCAT CACCACCCAG
    AACAAGGAGC GGGACTTCTC CATGTTTCTA AACCTGGACG AGGTGTTCAA GATCATGGAG
    CAGCTGGCCG ACGTGACGCT GCGGAGGCTG CTGGACAATG AGGGCTTCGA CCTGGACCCG
    GGTCTGCAGG AGCCCAGCCA GATCACCAAG AGGGACCTGG AAGCTAGGGC ACAGAATGAG
    TTCTTCCGGG CCTTCTTCAG GCTGCCAAGG CGGGAGAAGC TGCACGCCGT CCTGGACTGT
    TCCCTCTGGA CCCCATTCAG CCGCTGTCAC ACCGCCGGGC AGATGTTCAC CTCCGACAGT
    TACATCTGCT TTGCCAGCAA GGAGGCCGGC TGCTGCAAAG TCATCCTGCC GCTCAGAGAG
    GTTGTGAGCA TCGAGAAAAT GGAGGACACG AGTCTGCTGC CAAACCCCAT CATCGTCAGC
    ATCCGGAGCA AGATGGCCTT CCAGTTCATT GAGCTCAGAG ATCGTGACAG CCTGGTGGAG
    GGTCTGCTGG CGAGACTGAA GCTGGTGCAT GCCGACCACC CTGTGCACTA TGACACCACC
    CTGGATGAGG AGCTGACCTC ACCCGTGTTT CATTCAACAA GCTTGTGCAG TGGCAGCCAC
    AGGTTTGGGG GTCTTGAGAT GGTGTCTTCG CAAAATAGCG AGGAAAGGGA GAAAGAAACG
    AGCCCACGGA TGCACCCCGA GGCTTTCAGG CAGTCGGGCA GCCAGAGCCC GGACTCGGGA
    TTGTCCAGGG AACAGATCAA GGTGAGCCTC TGGAACGACC ACTTTGTGGA GTACGGCAGA
    ACAGTGTGCA TGTTCCGCAC AGAGAAGATC CGGAAGCTGG TGGCCATGGG GATCCCCGAG
    TCCCTGCGCG GCAGACTCTG GCTTCTTTTC TCAGATGCCG TCACGGACCT TGCCGCGCAT
    CCGGGTTACT ACGGCAACCT GGTGGAGGAG TCCATGGGAA AGTGTTGCCT GGTGACGGAG
    GAGATAGAGC GGGACCTGCA CCGTTCCTTG CCGGAGCACC CCGCCTTCCA AAACGAAACG
    GGCATCGCAG CGTTGAGGAG AGTCCTGACG GCCTATGCCC ACAGGAACCC CAAGATTGGG
    TACTGCCAGT CCATGAACAT CCTGACCTCC GTTCTGCTGC TGTATGCTAA GGAGGAGGAA
    GCCTTCTGGC TGCTGGTGGC TGTGTGTGAG CGGATGCTGC CCGACTACTT CAACCACCGA
    GTCATTGGGG CCCAGGTGGA CCAGTCCGTC TTCGAGGAGC TCATCAAGGA GCAGCTCCCT
    GAGCTGGCGG AGCACATGCA CGACCTCTCG GCCCTGGCGT CCATCTCCCT GTCCTGGTTC
    CTCACGCTCT TCCTCAGCAT CATGCCCCTG GAGAGTGCCG TGAACGTGGT CGACTGCTTC
    TTCTACGATG GCATCAAGGC CATCTTCCAG CTGGGACTGG CTGTGTTGGA GGCCACTGCC
    GAGGGCCTGT GCAGCAGCAA GGATGACAGC CAGGCCTTCA TGGTCCTCAG CAGGTTTCTG
    GACCACGTCA AGAACGAGGA CAGCCCGGGA CCCCCGATTG GCAGCCACCA CGCCTTCTTC
    TCTGACGACC AGGAGCCGTG TCCTGTGACC GACATCGCAG ACCTGATCCG GGACTCCTAC
    GAGAAATTTG GGGACCAGTC TGTGGCTCAG ATCGAGCACA TGCGCTGCAG GCACAGGATC
    CGAGTCCTCC AGGGCCACGA GGACACCACG AAGCAGAACG TGCTCCGCGT CGTTATCCCA
    GAAGTCTCTG TCCTTCCTGA AGACCTGGAG GAGCTCTACG ATTTATTCAA GAGAGAACAC
    ATGATGAGCT GCTACTGGGA GCAGCCTCGG CCCATGGCCC CACGCCACGA CCCCAGCAGG
    CCCTACGCAG AGCAGTACCG CATAGATGCC CAGCAGTTTG CACGCCTGTT CCAGCTCGTC
    TCGCCGTGGA CCTGCGGGGC CCACACGGAA ATCCTCGCAG AAAGGACTTT CAGGCTTCTG
    GACGAAAACA TGGACCACCT TATCGAATTC AAAGCATTCG TGAGCTGCCT CGATATTATG
    TATAATGGAG AAATGAATGA GAAAATTAAA CTGTTATATC GGCTTCATAT CCCTCCAGCA
    CTTACTGAAA ATGACCGAGA CAGCCAGTCA CCACTGAAGA ACCCTCTGTT GTCGACGTCA
    AGACCCCTGG TTTTCGGCAA GTCCAATGGT GATACAATTG ATTATCAGAA ACAGCTGAAG
    CAGATGATTA AAGATTTGGC CAAAGAGAAA GACAAAACTG AGAAAGAGTT GCCCAAAATG
    AGCCAGAGAG AGTTTATTCA GTTCTGTAAG ACTTTGTACA GTATGTTCCA CGAAGACCCG
    GAAGAGAATG ACCTGTACCA AGCCATCGCC ACAGTAACGA CGCTGCTGCT GCAGATTGGG
    GAGGTGGGGC AGCGCGGCAG CAGCTCTGGG AGCGGCTCCC AGGAAGGCGG GGAGGAGCTG
    CAGGCCTCGG CTCCTTCCCC CTCTTCTGAG CAGGACTTGG TGTTTGCCGA GACTGACACA
    GGGCAGAGTC CTCAGGCGTC CCAGGCATTT CCTGAGGAGG CACAAGGGGA CTGGACCGTC
    TCCCTTGAAC ATATTTTAGC CTCACTTCTG ACTGAACAGT CACTAGTCAA CTTTTTTGAA
    AAGCCACTGG ACATTAAATC CAAACTTGAA AACGCCAAGC TCAATCAGTA CAGCCTCAAG
    ACTTGTGAAA TGAGCCGCCA GTCACAGCCT GAGCTCAAGC TGAGCAGCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.