UNC13A cDNA ORF clone, Bos taurus(cattle)

The following UNC13A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNC13A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa51251 XM_010798163.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51251 XM_010806607.2
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51252 XM_010806608.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51254 XM_010806610.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51255 XM_010806611.2
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51255 XM_010798167.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51257 XM_010806613.2
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $881.30
$1259.00
OBa90182 XM_015463962.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa236377 XM_024994792.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa51253 XM_024994798.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa51256 XM_024994796.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90176 XM_015463956.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90176 XM_024994790.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90177 XM_024994791.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90177 XM_015463957.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90178 XM_024994793.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90178 XM_015463958.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90179 XM_015463959.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90179 XM_024994794.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90180 XM_015463960.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90180 XM_024994795.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90181 XM_024994797.1
Latest version!
Bos taurus unc-13 homolog A (UNC13A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa90181 XM_015463961.1
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa05033 XM_002688573.3
Latest version!
Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OBa51255 XM_010806611.1 Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa51251 XM_010806607.1 Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa51251
    Clone ID Related Accession (Same CDS sequence) XM_010798163.1 , XM_010806607.1 , XM_010806607.2
    Accession Version XM_010798163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796465.1
    CDS157..5268
    Translation

    Target ORF information:

    RefSeq Version XM_010798163.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51251
    Clone ID Related Accession (Same CDS sequence) XM_010798163.1 , XM_010806607.1 , XM_010806607.2
    Accession Version XM_010806607.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804909.1
    CDS157..5268
    Translation

    Target ORF information:

    RefSeq Version XM_010806607.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51251
    Clone ID Related Accession (Same CDS sequence) XM_010798163.1 , XM_010806607.1 , XM_010806607.2
    Accession Version XM_010806607.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804909.1
    CDS150..5261
    Translation

    Target ORF information:

    RefSeq Version XM_010806607.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806607.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GTCCATGGTG GAAAAGGGAC TAGGTTTACC CTTAGTGAAG ACATTTATCC TGAGAAGGGC
    TCGGGTGTGG AAGACCCTGT GGGTGAAGTC TCCGTCCACG TAGAGCTCTT CACTCATCCA
    GGAACTGGGG AACACAAGGT CACAGTGAAA GTGGTGGCTG CCAATGATCT CAAGTGGCAG
    ACTTCTGGCA TTTTCCGGCC ATTCATCGAG GTCAACATCA TTGGGCCCCA GCTTAGTGAC
    AAGAAACGCA AGTTTGCGAC CAAATCCAAG AACAACAGCT GGGCCCCCAA GTACAATGAG
    AGCTTTCAGT TCACGCTGAG CGCTGACACA GGCCCTGAGT GCTACGAGCT GCAGGTGTGC
    GTCAAGGACT ACTGCTTCGC GCGCGAGGAC CGCACGGTGG GGCTGGCCGT GCTGCAGCTA
    CGCGAGCTGG TCCAGCGCGG GAGCGCCGCC TGCTGGTTGC CCCTTGGCCG CCGCATCCAC
    ATGGACGACA CGGGCCTCAC GGTGCTGCGC ATCCTGTCGC AACGCAGCAA TGACGAGGTG
    GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC ACGCGCTCCG CCGAGGAAGG CGGTGCCGCG
    CCTGCCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51252
    Clone ID Related Accession (Same CDS sequence) XM_010806608.1
    Accession Version XM_010806608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGGGAACCCA GATGATCTTC AATGCAGCCA AGGAGCTGGG TCAGCTATCT
    AAACTCAAGG ACCACATGGT ACGAGAAGAA GCCAAGAGCT TGACCCCGAA GCAGTGTGCA
    GTTGTTGAGC TGGCCCTGGA TACTATCAAG CAATACTTCC ATGCAGGTGG CGTGGGCCTC
    AAGAAAACTT TCCTGGAGAA GAGCCCAGAC CTGCAGTCCC TGCGCTATGC CCTGTCCCTC
    TACACGCAGG CCACCGACCT GCTCATCAAG ACATTCGTGC AGACGCAGTC GGCCCAGGTC
    CATGGTGGAA AAGGGACTAG GTTTACCCTT AGTGAAGACA TTTATCCTGA GAAGGGCTCG
    GGTGTGGAAG ACCCTGTGGG TGAAGTCTCC GTCCACGTAG AGCTCTTCAC TCATCCAGGA
    ACTGGGGAAC ACAAGGTCAC AGTGAAAGTG GTGGCTGCCA ATGATCTCAA GTGGCAGACT
    TCTGGCATTT TCCGGCCATT CATCGAGGTC AACATCATTG GGCCCCAGCT TAGTGACAAG
    AAACGCAAGT TTGCGACCAA ATCCAAGAAC AACAGCTGGG CCCCCAAGTA CAATGAGAGC
    TTTCAGTTCA CGCTGAGCGC TGACACAGGC CCTGAGTGCT ACGAGCTGCA GGTGTGCGTC
    AAGGACTACT GCTTCGCGCG CGAGGACCGC ACGGTGGGGC TGGCCGTGCT GCAGCTACGC
    GAGCTGGTCC AGCGCGGGAG CGCCGCCTGC TGGTTGCCCC TTGGCCGCCG CATCCACATG
    GACGACACGG GCCTCACGGT GCTGCGCATC CTGTCGCAAC GCAGCAATGA CGAGGTGGCC
    AAGGAATTCG TCAAGCTCAA GTCGGACACG CGCTCCGCCG AGGAAGGCGG TGCCGCGCCT
    GCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804910.1
    CDS157..5265
    Translation

    Target ORF information:

    RefSeq Version XM_010806608.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGGGAACCCA GATGATCTTC AATGCAGCCA AGGAGCTGGG TCAGCTATCT
    AAACTCAAGG ACCACATGGT ACGAGAAGAA GCCAAGAGCT TGACCCCGAA GCAGTGTGCA
    GTTGTTGAGC TGGCCCTGGA TACTATCAAG CAATACTTCC ATGCAGGTGG CGTGGGCCTC
    AAGAAAACTT TCCTGGAGAA GAGCCCAGAC CTGCAGTCCC TGCGCTATGC CCTGTCCCTC
    TACACGCAGG CCACCGACCT GCTCATCAAG ACATTCGTGC AGACGCAGTC GGCCCAGGTC
    CATGGTGGAA AAGGGACTAG GTTTACCCTT AGTGAAGACA TTTATCCTGA GAAGGGCTCG
    GGTGTGGAAG ACCCTGTGGG TGAAGTCTCC GTCCACGTAG AGCTCTTCAC TCATCCAGGA
    ACTGGGGAAC ACAAGGTCAC AGTGAAAGTG GTGGCTGCCA ATGATCTCAA GTGGCAGACT
    TCTGGCATTT TCCGGCCATT CATCGAGGTC AACATCATTG GGCCCCAGCT TAGTGACAAG
    AAACGCAAGT TTGCGACCAA ATCCAAGAAC AACAGCTGGG CCCCCAAGTA CAATGAGAGC
    TTTCAGTTCA CGCTGAGCGC TGACACAGGC CCTGAGTGCT ACGAGCTGCA GGTGTGCGTC
    AAGGACTACT GCTTCGCGCG CGAGGACCGC ACGGTGGGGC TGGCCGTGCT GCAGCTACGC
    GAGCTGGTCC AGCGCGGGAG CGCCGCCTGC TGGTTGCCCC TTGGCCGCCG CATCCACATG
    GACGACACGG GCCTCACGGT GCTGCGCATC CTGTCGCAAC GCAGCAATGA CGAGGTGGCC
    AAGGAATTCG TCAAGCTCAA GTCGGACACG CGCTCCGCCG AGGAAGGCGG TGCCGCGCCT
    GCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51254
    Clone ID Related Accession (Same CDS sequence) XM_010806610.1
    Accession Version XM_010806610.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGG GAACCCAGAT GATCTTCAAT
    GCAGCCAAGG AGCTGGGTCA GCTATCTAAA CTCAAGGACC ACATGGTACG AGAAGAAGCC
    AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT GTTGAGCTGG CCCTGGATAC TATCAAGCAA
    TACTTCCATG CAGGTGGCGT GGGCCTCAAG AAAACTTTCC TGGAGAAGAG CCCAGACCTG
    CAGTCCCTGC GCTATGCCCT GTCCCTCTAC ACGCAGGCCA CCGACCTGCT CATCAAGACA
    TTCGTGCAGA CGCAGTCGGC CCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT
    GAAGACATTT ATCCTGAGAA GGGCTCGGGT GTGGAAGACC CTGTGGGTGA AGTCTCCGTC
    CACGTAGAGC TCTTCACTCA TCCAGGAACT GGGGAACACA AGGTCACAGT GAAAGTGGTG
    GCTGCCAATG ATCTCAAGTG GCAGACTTCT GGCATTTTCC GGCCATTCAT CGAGGTCAAC
    ATCATTGGGC CCCAGCTTAG TGACAAGAAA CGCAAGTTTG CGACCAAATC CAAGAACAAC
    AGCTGGGCCC CCAAGTACAA TGAGAGCTTT CAGTTCACGC TGAGCGCTGA CACAGGCCCT
    GAGTGCTACG AGCTGCAGGT GTGCGTCAAG GACTACTGCT TCGCGCGCGA GGACCGCACG
    GTGGGGCTGG CCGTGCTGCA GCTACGCGAG CTGGTCCAGC GCGGGAGCGC CGCCTGCTGG
    TTGCCCCTTG GCCGCCGCAT CCACATGGAC GACACGGGCC TCACGGTGCT GCGCATCCTG
    TCGCAACGCA GCAATGACGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC
    TCCGCCGAGG AAGGCGGTGC CGCGCCTGCC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804912.1
    CDS157..5232
    Translation

    Target ORF information:

    RefSeq Version XM_010806610.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806610.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGG GAACCCAGAT GATCTTCAAT
    GCAGCCAAGG AGCTGGGTCA GCTATCTAAA CTCAAGGACC ACATGGTACG AGAAGAAGCC
    AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT GTTGAGCTGG CCCTGGATAC TATCAAGCAA
    TACTTCCATG CAGGTGGCGT GGGCCTCAAG AAAACTTTCC TGGAGAAGAG CCCAGACCTG
    CAGTCCCTGC GCTATGCCCT GTCCCTCTAC ACGCAGGCCA CCGACCTGCT CATCAAGACA
    TTCGTGCAGA CGCAGTCGGC CCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT
    GAAGACATTT ATCCTGAGAA GGGCTCGGGT GTGGAAGACC CTGTGGGTGA AGTCTCCGTC
    CACGTAGAGC TCTTCACTCA TCCAGGAACT GGGGAACACA AGGTCACAGT GAAAGTGGTG
    GCTGCCAATG ATCTCAAGTG GCAGACTTCT GGCATTTTCC GGCCATTCAT CGAGGTCAAC
    ATCATTGGGC CCCAGCTTAG TGACAAGAAA CGCAAGTTTG CGACCAAATC CAAGAACAAC
    AGCTGGGCCC CCAAGTACAA TGAGAGCTTT CAGTTCACGC TGAGCGCTGA CACAGGCCCT
    GAGTGCTACG AGCTGCAGGT GTGCGTCAAG GACTACTGCT TCGCGCGCGA GGACCGCACG
    GTGGGGCTGG CCGTGCTGCA GCTACGCGAG CTGGTCCAGC GCGGGAGCGC CGCCTGCTGG
    TTGCCCCTTG GCCGCCGCAT CCACATGGAC GACACGGGCC TCACGGTGCT GCGCATCCTG
    TCGCAACGCA GCAATGACGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC
    TCCGCCGAGG AAGGCGGTGC CGCGCCTGCC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51255
    Clone ID Related Accession (Same CDS sequence) XM_010806611.2 , XM_010806611.1 , XM_010798167.1
    Accession Version XM_010806611.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804913.1
    CDS150..5204
    Translation

    Target ORF information:

    RefSeq Version XM_010806611.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51255
    Clone ID Related Accession (Same CDS sequence) XM_010806611.2 , XM_010806611.1 , XM_010798167.1
    Accession Version XM_010806611.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804913.1
    CDS157..5211
    Translation

    Target ORF information:

    RefSeq Version XM_010806611.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806611.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51255
    Clone ID Related Accession (Same CDS sequence) XM_010806611.2 , XM_010806611.1 , XM_010798167.1
    Accession Version XM_010798167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796469.1
    CDS157..5211
    Translation

    Target ORF information:

    RefSeq Version XM_010798167.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGCTCGGGTG TGGAAGACCC TGTGGGTGAA GTCTCCGTCC ACGTAGAGCT CTTCACTCAT
    CCAGGAACTG GGGAACACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA TCTCAAGTGG
    CAGACTTCTG GCATTTTCCG GCCATTCATC GAGGTCAACA TCATTGGGCC CCAGCTTAGT
    GACAAGAAAC GCAAGTTTGC GACCAAATCC AAGAACAACA GCTGGGCCCC CAAGTACAAT
    GAGAGCTTTC AGTTCACGCT GAGCGCTGAC ACAGGCCCTG AGTGCTACGA GCTGCAGGTG
    TGCGTCAAGG ACTACTGCTT CGCGCGCGAG GACCGCACGG TGGGGCTGGC CGTGCTGCAG
    CTACGCGAGC TGGTCCAGCG CGGGAGCGCC GCCTGCTGGT TGCCCCTTGG CCGCCGCATC
    CACATGGACG ACACGGGCCT CACGGTGCTG CGCATCCTGT CGCAACGCAG CAATGACGAG
    GTGGCCAAGG AATTCGTCAA GCTCAAGTCG GACACGCGCT CCGCCGAGGA AGGCGGTGCC
    GCGCCTGCCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51257
    Clone ID Related Accession (Same CDS sequence) XM_010806613.2
    Accession Version XM_010806613.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGTGCTGCTC TTAACCCAGA TCCCGGGTCC CCCAGACAGG CGCTTGCCCA TCTCAGATTC
    CAGCTGTGCC ACTCCGTGGG TGGGCGCCTC CCGCAACCGG GTGCCTCCTT GAGCCTCAGT
    TTTCTCATCT GTAAAATGAG AACAGTCAAG GACCCTAATT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804915.1
    CDS157..4821
    Translation

    Target ORF information:

    RefSeq Version XM_010806613.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806613.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGAAC CCAGATGATC TTCAATGCAG CCAAGGAGCT GGGTCAGCTA
    TCTAAACTCA AGGACCACAT GGTACGAGAA GAAGCCAAGA GCTTGACCCC GAAGCAGTGT
    GCAGTTGTTG AGCTGGCCCT GGATACTATC AAGCAATACT TCCATGCAGG TGGCGTGGGC
    CTCAAGAAAA CTTTCCTGGA GAAGAGCCCA GACCTGCAGT CCCTGCGCTA TGCCCTGTCC
    CTCTACACGC AGGCCACCGA CCTGCTCATC AAGACATTCG TGCAGACGCA GTCGGCCCAG
    GGTGCTGCTC TTAACCCAGA TCCCGGGTCC CCCAGACAGG CGCTTGCCCA TCTCAGATTC
    CAGCTGTGCC ACTCCGTGGG TGGGCGCCTC CCGCAACCGG GTGCCTCCTT GAGCCTCAGT
    TTTCTCATCT GTAAAATGAG AACAGTCAAG GACCCTAATT CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90182
    Clone ID Related Accession (Same CDS sequence) XM_015463962.1
    Accession Version XM_015463962.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACATACT CAAGCCCCAC AGGCAGCAGC CGCTACGCCT CCTCAGGGGA GCTGAGCCAA 
    GGCAGTTCAC AGCTGAGCGA GGACTTCGAC CCGGATGAGC AGAGCCTGCA GGGCTCTGAG
    CTAGAAGATG AGCGGGACCG GGACTCCTAC CACTCCTGCC ACAGCTCGGT CAGCTACCAC
    AAGGACTCGC CACGCTGGGA CCAGGACGAG GACGAATTGG ATGAGGAGCT GGATGAAGAG
    CTGGATGAGG ACCTGGAGGA CTTCCTGGAG GAGGAGGAGG AGCTACCTGA AGACGAGGAA
    GAGCTGGAGG AGGAGGAGGA GGTGCCAGAT GACATCCGAG GCTATGCCAT GCGAGAGGAG
    GTGGCCGTGG CCGAGCCCAA GGACTTCAAG CGTGTCAGCC TTCCACCTGC CGCTCCCGAG
    AAAGAGGAGC AGGCCCCAGC CATGCCCACT GAGGCCCCCG ACATACCCAA GGTGGCCCCT
    GAGCCAGCCG TGCCTGAGGA AGTGCCTGCA TCTGAGCAGG AGCCTGAGGC CCCCAAGGCT
    GAGGAGAGTT TCAGGCCCCG AGAAGATGAG GAAGGCCAAG AGGGTCAGGA CTCCATGTCT
    AGAGCCAAGG CCAACTGGCT GCGAGCCTTC AACAAAGTGC GGCTGCAGCT GCAGGAGGCC
    CGGGGAGAAG GAGAAATGTC CAAGTCCCTG TGGTTCAAAG GCGGCCCTGG CGGTGGTCTC
    ATCATCATTG ATAGCATGCC GGACATCCGC AAGAGGAAGC CGATCCCACT CGTGAGCGAC
    CTGGCCATGT CCCTTGTCCA GTCCAGAAAA GCAGGCATCA CCTCCGCCTT GGCATCCAGC
    ACTTTGAACA ACGAAGAGCT GAAAAATCAC GTTTACAAGA AGACCCTGCA AGCCTTAATC
    TACCCCATCT CGTGCACGAC GCCACACAAC TTCGAGGTGT GGACGGCTAC CACACCCACC
    TATTGCTACG AGTGTGAGGG GCTGCTGTGG GGCATCGCGC GGCAGGGCAT GCGCTGTACC
    GAGTGCGGTG TCAAGTGCCA CGAGAAGTGC CAGGACCTGC TCAATGCAGA CTGTCTGCAG
    CGGGCGGCGG AGAAGAGCTC CAAGCACGGG GCGGAGGACC GGACGCAGAA CATCATCATG
    GTGCTCAAGG ACCGCATGAA GATCCGGGAG CGCAACAAGC CCGAGATCTT CGAGCTCATC
    CAGGAGATCT TCGCGGTGAC CAAGACTGCA CACACGCAAC AGATGAAGGC AGTCAAGCAG
    AGCGTGCTGG ACGGAACATC CAAGTGGTCG GCCAAGATCA GCATCACTGT GGTCTGTGCC
    CAGGGGTTGC AGGCAAAGGA CAAGACGGGA TCCAGTGACC CCTATGTCAC CGTCCAGGTT
    GGGAAGACCA AGAAACGGAC AAAAACCATC TATGGAAACC TGAACCCCGT GTGGGAGGAG
    AACTTTCACT TTGAATGCCA CAACTCCTCA GACCGAATCA AGGTGCGCGT CTGGGATGAG
    GACGACGATA TCAAATCTCG CGTGAAGCAG CGGTTCAAAA GGGAATCCGA TGACTTCCTG
    GGTCAGACGA TCATCGAGGT GCGGACACTC AGTGGCGAGA TGGACGTGTG GTACAACCTG
    GACAAGCGGA CTGACAAATC TGCTGTGTCA GGTGCCATCC GGCTGCACAT CAGTGTGGAG
    ATCAAAGGTG AAGAGAAGGT GGCTCCATAC CACGTCCAGT ACACCTGTTT GCACGAGAAC
    CTGTTCCACT TCGTGACAGA CGTGCAGAAC AATGGGGTCG TGAAGATCCC AGATGCCAAG
    GGTGATGACG CCTGGAAAGT TTACTATGAT GAGACAGCCC AGGAGATTGT AGATGAATTT
    GCCATGCGCT ACGGCGTCGA GTCCATCTAC CAAGCCATGA CCCACTTTGC CTGCCTCTCG
    TCCAAGTACA TGTGTCCTGG GGTGCCTGCC GTCATGAGCA CCCTGCTTGC CAACATCAAC
    GCCTATTATG CTCACACCAC CGCCTCCACC AACGTGTCTG CCTCTGACCG CTTCGCCGCC
    TCCAACTTTG GGAAAGAGCG CTTTGTGAAG CTCCTGGACC AGCTGCATAA CTCCCTGCGG
    ATAGACCTCT CCATGTATCG GAATAACTTC CCAGCCAGCA GCCCAGAGAG ACTCCAGGAC
    CTCAAATCCA CTGTGGACCT TCTCACCAGC ATCACCTTCT TTCGGATGAA GGTACAAGAA
    CTCCAGAGCC CACCCCGAGC CAGCCAGGTG GTGAAGGACT GTGTGAAAGC CTGCCTCAAC
    TCTACTTATG AGTACATCTT CAACAATTGT CATGAACTCT ATAGTCGAGA GTACCAGACT
    GACCCGGCCA AGAAGGGGGA AGTTCCCCCA GAGGAACAAG GCCCCAGCAT CAAGAACCTC
    GACTTCTGGT CCAAGCTGAT CACTCTCATA GTGTCCATCA TTGAGGAAGA CAAAAATTCG
    TACACTCCCT GCCTCAACCA GTTTCCCCAG GAGTTGAACG TGGGTAAAAT CAGCGCTGAA
    GTGATGTGGA ATCTGTTTGC CCAGGATATG AAGTATGCAA TGGAGGAGCA TGATAAGCAT
    CGCCTGTGCA AGAGTGCCGA CTACATGAAC CTCCACTTCA AGGTCAAATG GCTCTACAAT
    GAGTATGTGG CTGAGCTGCC TGCCTTTAAG GACCGAGTGC CTGAGTATCC TGCGTGGTTT
    GAGCCCTTTG TCATCCAGTG GCTGGACGAG AATGAGGAAG TGTCCCGAGA TTTCTTGCAT
    GGAGCCCTGG AGAGAGACAA GAAGGATGGG TTCCAGCAGA CTTCAGAGCA TGCCCTGTTC
    TCCTGCTCTG TGGTGGATGT CTTCTCCCAG CTCAATCAGA GCTTTGAGAT CATCAAGAAA
    CTCGAGTGTC CTGACCCCCA GATTGTGGGG CACTACATGA GGCGCTTTGC CAAGACCATC
    AGTAATGTGC TCCTCCAGTA TGCAGACATC ATCTCCAAGG ACTTCGCTTC CTACTGCTCC
    AAGGAGAAGG AGAAAGTGCC CTGCATCCTC ATGAACAACA CTCAGCAGCT GAGAGTTCAG
    CTGGAGAAGA TGTTCGAAGC CATGGGAGGG AAGGAGCTGG ATGCCGAGGC TAGTGACATC
    TTGAAGGAGC TACAGGTGAA ACTCAACAAC GTCTTGGATG AGCTCAGCCG GGTGTTTGCT
    ACCAGCTTCC AGCCACACAT TGAAGACTGT GTCAAGCAGA TGGGTGACAT CCTTAGCCAG
    GTGAAAGGCA CAGGCAATGT GCCAGCCAGT GCCTGCAGCA GCGTGGCCCA GGACGCTGAC
    AATGTGCTAC AACCCATCAT GGACCTGCTG GATAGCAACC TGACTCTCTT TGCCAAAATC
    TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG AAGGAGTTAT GGAAGCTGGT CATGAACACC
    ATGGAGAAGA CCATCGTCCT GCCACCCCTC ACTGACCAGA CGATGATCGG CACCCTCTTG
    AGAAAACATG GCAAGGGATT AGAAAAGGGC AGGGTGAAAT TGCCAAGCCA CTCAGATGGA
    ACCCAGATGA TCTTCAATGC AGCCAAGGAG CTGGGTCAGC TATCTAAACT CAAGGACCAC
    ATGGTACGAG AAGAAGCCAA GAGCTTGACC CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC
    CTGGATACTA TCAAGCAATA CTTCCATGCA GGTGGCGTGG GCCTCAAGAA AACTTTCCTG
    GAGAAGAGCC CAGACCTGCA GTCCCTGCGC TATGCCCTGT CCCTCTACAC GCAGGCCACC
    GACCTGCTCA TCAAGACATT CGTGCAGACG CAGTCGGCCC AGGTCCATGG TGGAAAAGGG
    ACTAGGTTTA CCCTTAGTGA AGACATTTAT CCTGAGAAGG GCTCGGGTGT GGAAGACCCT
    GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC TTCACTCATC CAGGAACTGG GGAACACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAT CTCAAGTGGC AGACTTCTGG CATTTTCCGG
    CCATTCATCG AGGTCAACAT CATTGGGCCC CAGCTTAGTG ACAAGAAACG CAAGTTTGCG
    ACCAAATCCA AGAACAACAG CTGGGCCCCC AAGTACAATG AGAGCTTTCA GTTCACGCTG
    AGCGCTGACA CAGGCCCTGA GTGCTACGAG CTGCAGGTGT GCGTCAAGGA CTACTGCTTC
    GCGCGCGAGG ACCGCACGGT GGGGCTGGCC GTGCTGCAGC TACGCGAGCT GGTCCAGCGC
    GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC CGCCGCATCC ACATGGACGA CACGGGCCTC
    ACGGTGCTGC GCATCCTGTC GCAACGCAGC AATGACGAGG TGGCCAAGGA ATTCGTCAAG
    CTCAAGTCGG ACACGCGCTC CGCCGAGGAA GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319448.1
    CDS93..4526
    Translation

    Target ORF information:

    RefSeq Version XM_015463962.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463962.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACATACT CAAGCCCCAC AGGCAGCAGC CGCTACGCCT CCTCAGGGGA GCTGAGCCAA 
    GGCAGTTCAC AGCTGAGCGA GGACTTCGAC CCGGATGAGC AGAGCCTGCA GGGCTCTGAG
    CTAGAAGATG AGCGGGACCG GGACTCCTAC CACTCCTGCC ACAGCTCGGT CAGCTACCAC
    AAGGACTCGC CACGCTGGGA CCAGGACGAG GACGAATTGG ATGAGGAGCT GGATGAAGAG
    CTGGATGAGG ACCTGGAGGA CTTCCTGGAG GAGGAGGAGG AGCTACCTGA AGACGAGGAA
    GAGCTGGAGG AGGAGGAGGA GGTGCCAGAT GACATCCGAG GCTATGCCAT GCGAGAGGAG
    GTGGCCGTGG CCGAGCCCAA GGACTTCAAG CGTGTCAGCC TTCCACCTGC CGCTCCCGAG
    AAAGAGGAGC AGGCCCCAGC CATGCCCACT GAGGCCCCCG ACATACCCAA GGTGGCCCCT
    GAGCCAGCCG TGCCTGAGGA AGTGCCTGCA TCTGAGCAGG AGCCTGAGGC CCCCAAGGCT
    GAGGAGAGTT TCAGGCCCCG AGAAGATGAG GAAGGCCAAG AGGGTCAGGA CTCCATGTCT
    AGAGCCAAGG CCAACTGGCT GCGAGCCTTC AACAAAGTGC GGCTGCAGCT GCAGGAGGCC
    CGGGGAGAAG GAGAAATGTC CAAGTCCCTG TGGTTCAAAG GCGGCCCTGG CGGTGGTCTC
    ATCATCATTG ATAGCATGCC GGACATCCGC AAGAGGAAGC CGATCCCACT CGTGAGCGAC
    CTGGCCATGT CCCTTGTCCA GTCCAGAAAA GCAGGCATCA CCTCCGCCTT GGCATCCAGC
    ACTTTGAACA ACGAAGAGCT GAAAAATCAC GTTTACAAGA AGACCCTGCA AGCCTTAATC
    TACCCCATCT CGTGCACGAC GCCACACAAC TTCGAGGTGT GGACGGCTAC CACACCCACC
    TATTGCTACG AGTGTGAGGG GCTGCTGTGG GGCATCGCGC GGCAGGGCAT GCGCTGTACC
    GAGTGCGGTG TCAAGTGCCA CGAGAAGTGC CAGGACCTGC TCAATGCAGA CTGTCTGCAG
    CGGGCGGCGG AGAAGAGCTC CAAGCACGGG GCGGAGGACC GGACGCAGAA CATCATCATG
    GTGCTCAAGG ACCGCATGAA GATCCGGGAG CGCAACAAGC CCGAGATCTT CGAGCTCATC
    CAGGAGATCT TCGCGGTGAC CAAGACTGCA CACACGCAAC AGATGAAGGC AGTCAAGCAG
    AGCGTGCTGG ACGGAACATC CAAGTGGTCG GCCAAGATCA GCATCACTGT GGTCTGTGCC
    CAGGGGTTGC AGGCAAAGGA CAAGACGGGA TCCAGTGACC CCTATGTCAC CGTCCAGGTT
    GGGAAGACCA AGAAACGGAC AAAAACCATC TATGGAAACC TGAACCCCGT GTGGGAGGAG
    AACTTTCACT TTGAATGCCA CAACTCCTCA GACCGAATCA AGGTGCGCGT CTGGGATGAG
    GACGACGATA TCAAATCTCG CGTGAAGCAG CGGTTCAAAA GGGAATCCGA TGACTTCCTG
    GGTCAGACGA TCATCGAGGT GCGGACACTC AGTGGCGAGA TGGACGTGTG GTACAACCTG
    GACAAGCGGA CTGACAAATC TGCTGTGTCA GGTGCCATCC GGCTGCACAT CAGTGTGGAG
    ATCAAAGGTG AAGAGAAGGT GGCTCCATAC CACGTCCAGT ACACCTGTTT GCACGAGAAC
    CTGTTCCACT TCGTGACAGA CGTGCAGAAC AATGGGGTCG TGAAGATCCC AGATGCCAAG
    GGTGATGACG CCTGGAAAGT TTACTATGAT GAGACAGCCC AGGAGATTGT AGATGAATTT
    GCCATGCGCT ACGGCGTCGA GTCCATCTAC CAAGCCATGA CCCACTTTGC CTGCCTCTCG
    TCCAAGTACA TGTGTCCTGG GGTGCCTGCC GTCATGAGCA CCCTGCTTGC CAACATCAAC
    GCCTATTATG CTCACACCAC CGCCTCCACC AACGTGTCTG CCTCTGACCG CTTCGCCGCC
    TCCAACTTTG GGAAAGAGCG CTTTGTGAAG CTCCTGGACC AGCTGCATAA CTCCCTGCGG
    ATAGACCTCT CCATGTATCG GAATAACTTC CCAGCCAGCA GCCCAGAGAG ACTCCAGGAC
    CTCAAATCCA CTGTGGACCT TCTCACCAGC ATCACCTTCT TTCGGATGAA GGTACAAGAA
    CTCCAGAGCC CACCCCGAGC CAGCCAGGTG GTGAAGGACT GTGTGAAAGC CTGCCTCAAC
    TCTACTTATG AGTACATCTT CAACAATTGT CATGAACTCT ATAGTCGAGA GTACCAGACT
    GACCCGGCCA AGAAGGGGGA AGTTCCCCCA GAGGAACAAG GCCCCAGCAT CAAGAACCTC
    GACTTCTGGT CCAAGCTGAT CACTCTCATA GTGTCCATCA TTGAGGAAGA CAAAAATTCG
    TACACTCCCT GCCTCAACCA GTTTCCCCAG GAGTTGAACG TGGGTAAAAT CAGCGCTGAA
    GTGATGTGGA ATCTGTTTGC CCAGGATATG AAGTATGCAA TGGAGGAGCA TGATAAGCAT
    CGCCTGTGCA AGAGTGCCGA CTACATGAAC CTCCACTTCA AGGTCAAATG GCTCTACAAT
    GAGTATGTGG CTGAGCTGCC TGCCTTTAAG GACCGAGTGC CTGAGTATCC TGCGTGGTTT
    GAGCCCTTTG TCATCCAGTG GCTGGACGAG AATGAGGAAG TGTCCCGAGA TTTCTTGCAT
    GGAGCCCTGG AGAGAGACAA GAAGGATGGG TTCCAGCAGA CTTCAGAGCA TGCCCTGTTC
    TCCTGCTCTG TGGTGGATGT CTTCTCCCAG CTCAATCAGA GCTTTGAGAT CATCAAGAAA
    CTCGAGTGTC CTGACCCCCA GATTGTGGGG CACTACATGA GGCGCTTTGC CAAGACCATC
    AGTAATGTGC TCCTCCAGTA TGCAGACATC ATCTCCAAGG ACTTCGCTTC CTACTGCTCC
    AAGGAGAAGG AGAAAGTGCC CTGCATCCTC ATGAACAACA CTCAGCAGCT GAGAGTTCAG
    CTGGAGAAGA TGTTCGAAGC CATGGGAGGG AAGGAGCTGG ATGCCGAGGC TAGTGACATC
    TTGAAGGAGC TACAGGTGAA ACTCAACAAC GTCTTGGATG AGCTCAGCCG GGTGTTTGCT
    ACCAGCTTCC AGCCACACAT TGAAGACTGT GTCAAGCAGA TGGGTGACAT CCTTAGCCAG
    GTGAAAGGCA CAGGCAATGT GCCAGCCAGT GCCTGCAGCA GCGTGGCCCA GGACGCTGAC
    AATGTGCTAC AACCCATCAT GGACCTGCTG GATAGCAACC TGACTCTCTT TGCCAAAATC
    TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG AAGGAGTTAT GGAAGCTGGT CATGAACACC
    ATGGAGAAGA CCATCGTCCT GCCACCCCTC ACTGACCAGA CGATGATCGG CACCCTCTTG
    AGAAAACATG GCAAGGGATT AGAAAAGGGC AGGGTGAAAT TGCCAAGCCA CTCAGATGGA
    ACCCAGATGA TCTTCAATGC AGCCAAGGAG CTGGGTCAGC TATCTAAACT CAAGGACCAC
    ATGGTACGAG AAGAAGCCAA GAGCTTGACC CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC
    CTGGATACTA TCAAGCAATA CTTCCATGCA GGTGGCGTGG GCCTCAAGAA AACTTTCCTG
    GAGAAGAGCC CAGACCTGCA GTCCCTGCGC TATGCCCTGT CCCTCTACAC GCAGGCCACC
    GACCTGCTCA TCAAGACATT CGTGCAGACG CAGTCGGCCC AGGTCCATGG TGGAAAAGGG
    ACTAGGTTTA CCCTTAGTGA AGACATTTAT CCTGAGAAGG GCTCGGGTGT GGAAGACCCT
    GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC TTCACTCATC CAGGAACTGG GGAACACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAT CTCAAGTGGC AGACTTCTGG CATTTTCCGG
    CCATTCATCG AGGTCAACAT CATTGGGCCC CAGCTTAGTG ACAAGAAACG CAAGTTTGCG
    ACCAAATCCA AGAACAACAG CTGGGCCCCC AAGTACAATG AGAGCTTTCA GTTCACGCTG
    AGCGCTGACA CAGGCCCTGA GTGCTACGAG CTGCAGGTGT GCGTCAAGGA CTACTGCTTC
    GCGCGCGAGG ACCGCACGGT GGGGCTGGCC GTGCTGCAGC TACGCGAGCT GGTCCAGCGC
    GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC CGCCGCATCC ACATGGACGA CACGGGCCTC
    ACGGTGCTGC GCATCCTGTC GCAACGCAGC AATGACGAGG TGGCCAAGGA ATTCGTCAAG
    CTCAAGTCGG ACACGCGCTC CGCCGAGGAA GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236377
    Clone ID Related Accession (Same CDS sequence) XM_024994792.1
    Accession Version XM_024994792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGA TGATCGGCAC CCTCTTGAGA AAACATGGCA AGGGATTAGA AAAGGGCAGG
    GTGAAATTGC CAAGCCACTC AGATGGAACC CAGATGATCT TCAATGCAGC CAAGGAGCTG
    GGTCAGCTAT CTAAACTCAA GGACCACATG GTACGAGAAG AAGCCAAGAG CTTGACCCCG
    AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG GATACTATCA AGCAATACTT CCATGCAGGT
    GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT
    GCCCTGTCCC TCTACACGCA GGCCACCGAC CTGCTCATCA AGACATTCGT GCAGACGCAG
    TCGGCCCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CATTTATCCT
    GAGAAGGGCT CGGGTGTGGA AGACCCTGTG GGTGAAGTCT CCGTCCACGT AGAGCTCTTC
    ACTCATCCAG GAACTGGGGA ACACAAGGTC ACAGTGAAAG TGGTGGCTGC CAATGATCTC
    AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA TTCATCGAGG TCAACATCAT TGGGCCCCAG
    CTTAGTGACA AGAAACGCAA GTTTGCGACC AAATCCAAGA ACAACAGCTG GGCCCCCAAG
    TACAATGAGA GCTTTCAGTT CACGCTGAGC GCTGACACAG GCCCTGAGTG CTACGAGCTG
    CAGGTGTGCG TCAAGGACTA CTGCTTCGCG CGCGAGGACC GCACGGTGGG GCTGGCCGTG
    CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC
    CGCATCCACA TGGACGACAC GGGCCTCACG GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT
    GACGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCCGC CGAGGAAGGC
    GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850560.1
    CDS150..5330
    Translation

    Target ORF information:

    RefSeq Version XM_024994792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGA TGATCGGCAC CCTCTTGAGA AAACATGGCA AGGGATTAGA AAAGGGCAGG
    GTGAAATTGC CAAGCCACTC AGATGGAACC CAGATGATCT TCAATGCAGC CAAGGAGCTG
    GGTCAGCTAT CTAAACTCAA GGACCACATG GTACGAGAAG AAGCCAAGAG CTTGACCCCG
    AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG GATACTATCA AGCAATACTT CCATGCAGGT
    GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT
    GCCCTGTCCC TCTACACGCA GGCCACCGAC CTGCTCATCA AGACATTCGT GCAGACGCAG
    TCGGCCCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CATTTATCCT
    GAGAAGGGCT CGGGTGTGGA AGACCCTGTG GGTGAAGTCT CCGTCCACGT AGAGCTCTTC
    ACTCATCCAG GAACTGGGGA ACACAAGGTC ACAGTGAAAG TGGTGGCTGC CAATGATCTC
    AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA TTCATCGAGG TCAACATCAT TGGGCCCCAG
    CTTAGTGACA AGAAACGCAA GTTTGCGACC AAATCCAAGA ACAACAGCTG GGCCCCCAAG
    TACAATGAGA GCTTTCAGTT CACGCTGAGC GCTGACACAG GCCCTGAGTG CTACGAGCTG
    CAGGTGTGCG TCAAGGACTA CTGCTTCGCG CGCGAGGACC GCACGGTGGG GCTGGCCGTG
    CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC
    CGCATCCACA TGGACGACAC GGGCCTCACG GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT
    GACGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCCGC CGAGGAAGGC
    GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51253
    Clone ID Related Accession (Same CDS sequence) XM_024994798.1
    Accession Version XM_024994798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGG GAACCCAGAT GATCTTCAAT GCAGCCAAGG AGCTGGGTCA GCTATCTAAA
    CTCAAGGACC ACATGGTACG AGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT
    GTTGAGCTGG CCCTGGATAC TATCAAGCAA TACTTCCATG CAGGTGGCGT GGGCCTCAAG
    AAAACTTTCC TGGAGAAGAG CCCAGACCTG CAGTCCCTGC GCTATGCCCT GTCCCTCTAC
    ACGCAGGCCA CCGACCTGCT CATCAAGACA TTCGTGCAGA CGCAGTCGGC CCAGGTCCAT
    GGTGGAAAAG GGACTAGGTT TACCCTTAGT GAAGACATTT ATCCTGAGAA GGGCTCGGGT
    GTGGAAGACC CTGTGGGTGA AGTCTCCGTC CACGTAGAGC TCTTCACTCA TCCAGGAACT
    GGGGAACACA AGGTCACAGT GAAAGTGGTG GCTGCCAATG ATCTCAAGTG GCAGACTTCT
    GGCATTTTCC GGCCATTCAT CGAGGTCAAC ATCATTGGGC CCCAGCTTAG TGACAAGAAA
    CGCAAGTTTG CGACCAAATC CAAGAACAAC AGCTGGGCCC CCAAGTACAA TGAGAGCTTT
    CAGTTCACGC TGAGCGCTGA CACAGGCCCT GAGTGCTACG AGCTGCAGGT GTGCGTCAAG
    GACTACTGCT TCGCGCGCGA GGACCGCACG GTGGGGCTGG CCGTGCTGCA GCTACGCGAG
    CTGGTCCAGC GCGGGAGCGC CGCCTGCTGG TTGCCCCTTG GCCGCCGCAT CCACATGGAC
    GACACGGGCC TCACGGTGCT GCGCATCCTG TCGCAACGCA GCAATGACGA GGTGGCCAAG
    GAATTCGTCA AGCTCAAGTC GGACACGCGC TCCGCCGAGG AAGGCGGTGC CGCGCCTGCC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850566.1
    CDS150..5255
    Translation

    Target ORF information:

    RefSeq Version XM_024994798.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGG GAACCCAGAT GATCTTCAAT GCAGCCAAGG AGCTGGGTCA GCTATCTAAA
    CTCAAGGACC ACATGGTACG AGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT
    GTTGAGCTGG CCCTGGATAC TATCAAGCAA TACTTCCATG CAGGTGGCGT GGGCCTCAAG
    AAAACTTTCC TGGAGAAGAG CCCAGACCTG CAGTCCCTGC GCTATGCCCT GTCCCTCTAC
    ACGCAGGCCA CCGACCTGCT CATCAAGACA TTCGTGCAGA CGCAGTCGGC CCAGGTCCAT
    GGTGGAAAAG GGACTAGGTT TACCCTTAGT GAAGACATTT ATCCTGAGAA GGGCTCGGGT
    GTGGAAGACC CTGTGGGTGA AGTCTCCGTC CACGTAGAGC TCTTCACTCA TCCAGGAACT
    GGGGAACACA AGGTCACAGT GAAAGTGGTG GCTGCCAATG ATCTCAAGTG GCAGACTTCT
    GGCATTTTCC GGCCATTCAT CGAGGTCAAC ATCATTGGGC CCCAGCTTAG TGACAAGAAA
    CGCAAGTTTG CGACCAAATC CAAGAACAAC AGCTGGGCCC CCAAGTACAA TGAGAGCTTT
    CAGTTCACGC TGAGCGCTGA CACAGGCCCT GAGTGCTACG AGCTGCAGGT GTGCGTCAAG
    GACTACTGCT TCGCGCGCGA GGACCGCACG GTGGGGCTGG CCGTGCTGCA GCTACGCGAG
    CTGGTCCAGC GCGGGAGCGC CGCCTGCTGG TTGCCCCTTG GCCGCCGCAT CCACATGGAC
    GACACGGGCC TCACGGTGCT GCGCATCCTG TCGCAACGCA GCAATGACGA GGTGGCCAAG
    GAATTCGTCA AGCTCAAGTC GGACACGCGC TCCGCCGAGG AAGGCGGTGC CGCGCCTGCC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51256
    Clone ID Related Accession (Same CDS sequence) XM_024994796.1
    Accession Version XM_024994796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGG GAACCCAGAT GATCTTCAAT GCAGCCAAGG AGCTGGGTCA GCTATCTAAA
    CTCAAGGACC ACATGGTACG AGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT
    GTTGAGCTGG CCCTGGATAC TATCAAGCAA TACTTCCATG CAGGTGGCGT GGGCCTCAAG
    AAAACTTTCC TGGAGAAGAG CCCAGACCTG CAGTCCCTGC GCTATGCCCT GTCCCTCTAC
    ACGCAGGCCA CCGACCTGCT CATCAAGACA TTCGTGCAGA CGCAGTCGGC CCAGGGCTCG
    GGTGTGGAAG ACCCTGTGGG TGAAGTCTCC GTCCACGTAG AGCTCTTCAC TCATCCAGGA
    ACTGGGGAAC ACAAGGTCAC AGTGAAAGTG GTGGCTGCCA ATGATCTCAA GTGGCAGACT
    TCTGGCATTT TCCGGCCATT CATCGAGGTC AACATCATTG GGCCCCAGCT TAGTGACAAG
    AAACGCAAGT TTGCGACCAA ATCCAAGAAC AACAGCTGGG CCCCCAAGTA CAATGAGAGC
    TTTCAGTTCA CGCTGAGCGC TGACACAGGC CCTGAGTGCT ACGAGCTGCA GGTGTGCGTC
    AAGGACTACT GCTTCGCGCG CGAGGACCGC ACGGTGGGGC TGGCCGTGCT GCAGCTACGC
    GAGCTGGTCC AGCGCGGGAG CGCCGCCTGC TGGTTGCCCC TTGGCCGCCG CATCCACATG
    GACGACACGG GCCTCACGGT GCTGCGCATC CTGTCGCAAC GCAGCAATGA CGAGGTGGCC
    AAGGAATTCG TCAAGCTCAA GTCGGACACG CGCTCCGCCG AGGAAGGCGG TGCCGCGCCT
    GCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850564.1
    CDS150..5198
    Translation

    Target ORF information:

    RefSeq Version XM_024994796.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGTCCC TTGTCCAGTC CAGAAAAGCA
    GGCATCACCT CCGCCTTGGC ATCCAGCACT TTGAACAACG AAGAGCTGAA AAATCACGTT
    TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCGT GCACGACGCC ACACAACTTC
    GAGGTGTGGA CGGCTACCAC ACCCACCTAT TGCTACGAGT GTGAGGGGCT GCTGTGGGGC
    ATCGCGCGGC AGGGCATGCG CTGTACCGAG TGCGGTGTCA AGTGCCACGA GAAGTGCCAG
    GACCTGCTCA ATGCAGACTG TCTGCAGCGG GCGGCGGAGA AGAGCTCCAA GCACGGGGCG
    GAGGACCGGA CGCAGAACAT CATCATGGTG CTCAAGGACC GCATGAAGAT CCGGGAGCGC
    AACAAGCCCG AGATCTTCGA GCTCATCCAG GAGATCTTCG CGGTGACCAA GACTGCACAC
    ACGCAACAGA TGAAGGCAGT CAAGCAGAGC GTGCTGGACG GAACATCCAA GTGGTCGGCC
    AAGATCAGCA TCACTGTGGT CTGTGCCCAG GGGTTGCAGG CAAAGGACAA GACGGGATCC
    AGTGACCCCT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AACGGACAAA AACCATCTAT
    GGAAACCTGA ACCCCGTGTG GGAGGAGAAC TTTCACTTTG AATGCCACAA CTCCTCAGAC
    CGAATCAAGG TGCGCGTCTG GGATGAGGAC GACGATATCA AATCTCGCGT GAAGCAGCGG
    TTCAAAAGGG AATCCGATGA CTTCCTGGGT CAGACGATCA TCGAGGTGCG GACACTCAGT
    GGCGAGATGG ACGTGTGGTA CAACCTGGAC AAGCGGACTG ACAAATCTGC TGTGTCAGGT
    GCCATCCGGC TGCACATCAG TGTGGAGATC AAAGGTGAAG AGAAGGTGGC TCCATACCAC
    GTCCAGTACA CCTGTTTGCA CGAGAACCTG TTCCACTTCG TGACAGACGT GCAGAACAAT
    GGGGTCGTGA AGATCCCAGA TGCCAAGGGT GATGACGCCT GGAAAGTTTA CTATGATGAG
    ACAGCCCAGG AGATTGTAGA TGAATTTGCC ATGCGCTACG GCGTCGAGTC CATCTACCAA
    GCCATGACCC ACTTTGCCTG CCTCTCGTCC AAGTACATGT GTCCTGGGGT GCCTGCCGTC
    ATGAGCACCC TGCTTGCCAA CATCAACGCC TATTATGCTC ACACCACCGC CTCCACCAAC
    GTGTCTGCCT CTGACCGCTT CGCCGCCTCC AACTTTGGGA AAGAGCGCTT TGTGAAGCTC
    CTGGACCAGC TGCATAACTC CCTGCGGATA GACCTCTCCA TGTATCGGAA TAACTTCCCA
    GCCAGCAGCC CAGAGAGACT CCAGGACCTC AAATCCACTG TGGACCTTCT CACCAGCATC
    ACCTTCTTTC GGATGAAGGT ACAAGAACTC CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG
    AAGGACTGTG TGAAAGCCTG CCTCAACTCT ACTTATGAGT ACATCTTCAA CAATTGTCAT
    GAACTCTATA GTCGAGAGTA CCAGACTGAC CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG
    GAACAAGGCC CCAGCATCAA GAACCTCGAC TTCTGGTCCA AGCTGATCAC TCTCATAGTG
    TCCATCATTG AGGAAGACAA AAATTCGTAC ACTCCCTGCC TCAACCAGTT TCCCCAGGAG
    TTGAACGTGG GTAAAATCAG CGCTGAAGTG ATGTGGAATC TGTTTGCCCA GGATATGAAG
    TATGCAATGG AGGAGCATGA TAAGCATCGC CTGTGCAAGA GTGCCGACTA CATGAACCTC
    CACTTCAAGG TCAAATGGCT CTACAATGAG TATGTGGCTG AGCTGCCTGC CTTTAAGGAC
    CGAGTGCCTG AGTATCCTGC GTGGTTTGAG CCCTTTGTCA TCCAGTGGCT GGACGAGAAT
    GAGGAAGTGT CCCGAGATTT CTTGCATGGA GCCCTGGAGA GAGACAAGAA GGATGGGTTC
    CAGCAGACTT CAGAGCATGC CCTGTTCTCC TGCTCTGTGG TGGATGTCTT CTCCCAGCTC
    AATCAGAGCT TTGAGATCAT CAAGAAACTC GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC
    TACATGAGGC GCTTTGCCAA GACCATCAGT AATGTGCTCC TCCAGTATGC AGACATCATC
    TCCAAGGACT TCGCTTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG
    AACAACACTC AGCAGCTGAG AGTTCAGCTG GAGAAGATGT TCGAAGCCAT GGGAGGGAAG
    GAGCTGGATG CCGAGGCTAG TGACATCTTG AAGGAGCTAC AGGTGAAACT CAACAACGTC
    TTGGATGAGC TCAGCCGGGT GTTTGCTACC AGCTTCCAGC CACACATTGA AGACTGTGTC
    AAGCAGATGG GTGACATCCT TAGCCAGGTG AAAGGCACAG GCAATGTGCC AGCCAGTGCC
    TGCAGCAGCG TGGCCCAGGA CGCTGACAAT GTGCTACAAC CCATCATGGA CCTGCTGGAT
    AGCAACCTGA CTCTCTTTGC CAAAATCTGT GAGAAGACGG TGCTGAAGCG GGTGCTGAAG
    GAGTTATGGA AGCTGGTCAT GAACACCATG GAGAAGACCA TCGTCCTGCC ACCCCTCACT
    GACCAGACGG GAACCCAGAT GATCTTCAAT GCAGCCAAGG AGCTGGGTCA GCTATCTAAA
    CTCAAGGACC ACATGGTACG AGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCAGTT
    GTTGAGCTGG CCCTGGATAC TATCAAGCAA TACTTCCATG CAGGTGGCGT GGGCCTCAAG
    AAAACTTTCC TGGAGAAGAG CCCAGACCTG CAGTCCCTGC GCTATGCCCT GTCCCTCTAC
    ACGCAGGCCA CCGACCTGCT CATCAAGACA TTCGTGCAGA CGCAGTCGGC CCAGGGCTCG
    GGTGTGGAAG ACCCTGTGGG TGAAGTCTCC GTCCACGTAG AGCTCTTCAC TCATCCAGGA
    ACTGGGGAAC ACAAGGTCAC AGTGAAAGTG GTGGCTGCCA ATGATCTCAA GTGGCAGACT
    TCTGGCATTT TCCGGCCATT CATCGAGGTC AACATCATTG GGCCCCAGCT TAGTGACAAG
    AAACGCAAGT TTGCGACCAA ATCCAAGAAC AACAGCTGGG CCCCCAAGTA CAATGAGAGC
    TTTCAGTTCA CGCTGAGCGC TGACACAGGC CCTGAGTGCT ACGAGCTGCA GGTGTGCGTC
    AAGGACTACT GCTTCGCGCG CGAGGACCGC ACGGTGGGGC TGGCCGTGCT GCAGCTACGC
    GAGCTGGTCC AGCGCGGGAG CGCCGCCTGC TGGTTGCCCC TTGGCCGCCG CATCCACATG
    GACGACACGG GCCTCACGGT GCTGCGCATC CTGTCGCAAC GCAGCAATGA CGAGGTGGCC
    AAGGAATTCG TCAAGCTCAA GTCGGACACG CGCTCCGCCG AGGAAGGCGG TGCCGCGCCT
    GCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90176
    Clone ID Related Accession (Same CDS sequence) XM_015463956.1 , XM_024994790.1
    Accession Version XM_015463956.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGTCCA TGGTGGAAAA GGGACTAGGT TTACCCTTAG TGAAGACATT
    TATCCTGAGA AGGGCTCGGG TGTGGAAGAC CCTGTGGGTG AAGTCTCCGT CCACGTAGAG
    CTCTTCACTC ATCCAGGAAC TGGGGAACAC AAGGTCACAG TGAAAGTGGT GGCTGCCAAT
    GATCTCAAGT GGCAGACTTC TGGCATTTTC CGGCCATTCA TCGAGGTCAA CATCATTGGG
    CCCCAGCTTA GTGACAAGAA ACGCAAGTTT GCGACCAAAT CCAAGAACAA CAGCTGGGCC
    CCCAAGTACA ATGAGAGCTT TCAGTTCACG CTGAGCGCTG ACACAGGCCC TGAGTGCTAC
    GAGCTGCAGG TGTGCGTCAA GGACTACTGC TTCGCGCGCG AGGACCGCAC GGTGGGGCTG
    GCCGTGCTGC AGCTACGCGA GCTGGTCCAG CGCGGGAGCG CCGCCTGCTG GTTGCCCCTT
    GGCCGCCGCA TCCACATGGA CGACACGGGC CTCACGGTGC TGCGCATCCT GTCGCAACGC
    AGCAATGACG AGGTGGCCAA GGAATTCGTC AAGCTCAAGT CGGACACGCG CTCCGCCGAG
    GAAGGCGGTG CCGCGCCTGC CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319442.1
    CDS157..5343
    Translation

    Target ORF information:

    RefSeq Version XM_015463956.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGTCCA TGGTGGAAAA GGGACTAGGT TTACCCTTAG TGAAGACATT
    TATCCTGAGA AGGGCTCGGG TGTGGAAGAC CCTGTGGGTG AAGTCTCCGT CCACGTAGAG
    CTCTTCACTC ATCCAGGAAC TGGGGAACAC AAGGTCACAG TGAAAGTGGT GGCTGCCAAT
    GATCTCAAGT GGCAGACTTC TGGCATTTTC CGGCCATTCA TCGAGGTCAA CATCATTGGG
    CCCCAGCTTA GTGACAAGAA ACGCAAGTTT GCGACCAAAT CCAAGAACAA CAGCTGGGCC
    CCCAAGTACA ATGAGAGCTT TCAGTTCACG CTGAGCGCTG ACACAGGCCC TGAGTGCTAC
    GAGCTGCAGG TGTGCGTCAA GGACTACTGC TTCGCGCGCG AGGACCGCAC GGTGGGGCTG
    GCCGTGCTGC AGCTACGCGA GCTGGTCCAG CGCGGGAGCG CCGCCTGCTG GTTGCCCCTT
    GGCCGCCGCA TCCACATGGA CGACACGGGC CTCACGGTGC TGCGCATCCT GTCGCAACGC
    AGCAATGACG AGGTGGCCAA GGAATTCGTC AAGCTCAAGT CGGACACGCG CTCCGCCGAG
    GAAGGCGGTG CCGCGCCTGC CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90176
    Clone ID Related Accession (Same CDS sequence) XM_015463956.1 , XM_024994790.1
    Accession Version XM_024994790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGTCCA TGGTGGAAAA GGGACTAGGT TTACCCTTAG TGAAGACATT
    TATCCTGAGA AGGGCTCGGG TGTGGAAGAC CCTGTGGGTG AAGTCTCCGT CCACGTAGAG
    CTCTTCACTC ATCCAGGAAC TGGGGAACAC AAGGTCACAG TGAAAGTGGT GGCTGCCAAT
    GATCTCAAGT GGCAGACTTC TGGCATTTTC CGGCCATTCA TCGAGGTCAA CATCATTGGG
    CCCCAGCTTA GTGACAAGAA ACGCAAGTTT GCGACCAAAT CCAAGAACAA CAGCTGGGCC
    CCCAAGTACA ATGAGAGCTT TCAGTTCACG CTGAGCGCTG ACACAGGCCC TGAGTGCTAC
    GAGCTGCAGG TGTGCGTCAA GGACTACTGC TTCGCGCGCG AGGACCGCAC GGTGGGGCTG
    GCCGTGCTGC AGCTACGCGA GCTGGTCCAG CGCGGGAGCG CCGCCTGCTG GTTGCCCCTT
    GGCCGCCGCA TCCACATGGA CGACACGGGC CTCACGGTGC TGCGCATCCT GTCGCAACGC
    AGCAATGACG AGGTGGCCAA GGAATTCGTC AAGCTCAAGT CGGACACGCG CTCCGCCGAG
    GAAGGCGGTG CCGCGCCTGC CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850558.1
    CDS150..5336
    Translation

    Target ORF information:

    RefSeq Version XM_024994790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGTCCA TGGTGGAAAA GGGACTAGGT TTACCCTTAG TGAAGACATT
    TATCCTGAGA AGGGCTCGGG TGTGGAAGAC CCTGTGGGTG AAGTCTCCGT CCACGTAGAG
    CTCTTCACTC ATCCAGGAAC TGGGGAACAC AAGGTCACAG TGAAAGTGGT GGCTGCCAAT
    GATCTCAAGT GGCAGACTTC TGGCATTTTC CGGCCATTCA TCGAGGTCAA CATCATTGGG
    CCCCAGCTTA GTGACAAGAA ACGCAAGTTT GCGACCAAAT CCAAGAACAA CAGCTGGGCC
    CCCAAGTACA ATGAGAGCTT TCAGTTCACG CTGAGCGCTG ACACAGGCCC TGAGTGCTAC
    GAGCTGCAGG TGTGCGTCAA GGACTACTGC TTCGCGCGCG AGGACCGCAC GGTGGGGCTG
    GCCGTGCTGC AGCTACGCGA GCTGGTCCAG CGCGGGAGCG CCGCCTGCTG GTTGCCCCTT
    GGCCGCCGCA TCCACATGGA CGACACGGGC CTCACGGTGC TGCGCATCCT GTCGCAACGC
    AGCAATGACG AGGTGGCCAA GGAATTCGTC AAGCTCAAGT CGGACACGCG CTCCGCCGAG
    GAAGGCGGTG CCGCGCCTGC CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90177
    Clone ID Related Accession (Same CDS sequence) XM_024994791.1 , XM_015463957.1
    Accession Version XM_024994791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGATGATCGG CACCCTCTTG AGAAAACATG GCAAGGGATT AGAAAAGGGC
    AGGGTGAAAT TGCCAAGCCA CTCAGATGGA ACCCAGATGA TCTTCAATGC AGCCAAGGAG
    CTGGGTCAGC TATCTAAACT CAAGGACCAC ATGGTACGAG AAGAAGCCAA GAGCTTGACC
    CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC CTGGATACTA TCAAGCAATA CTTCCATGCA
    GGTGGCGTGG GCCTCAAGAA AACTTTCCTG GAGAAGAGCC CAGACCTGCA GTCCCTGCGC
    TATGCCCTGT CCCTCTACAC GCAGGCCACC GACCTGCTCA TCAAGACATT CGTGCAGACG
    CAGTCGGCCC AGGTCCATGG TGGAAAAGGG ACTAGGTTTA CCCTTAGTGA AGACATTTAT
    CCTGAGAAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850559.1
    CDS150..5333
    Translation

    Target ORF information:

    RefSeq Version XM_024994791.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGATGATCGG CACCCTCTTG AGAAAACATG GCAAGGGATT AGAAAAGGGC
    AGGGTGAAAT TGCCAAGCCA CTCAGATGGA ACCCAGATGA TCTTCAATGC AGCCAAGGAG
    CTGGGTCAGC TATCTAAACT CAAGGACCAC ATGGTACGAG AAGAAGCCAA GAGCTTGACC
    CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC CTGGATACTA TCAAGCAATA CTTCCATGCA
    GGTGGCGTGG GCCTCAAGAA AACTTTCCTG GAGAAGAGCC CAGACCTGCA GTCCCTGCGC
    TATGCCCTGT CCCTCTACAC GCAGGCCACC GACCTGCTCA TCAAGACATT CGTGCAGACG
    CAGTCGGCCC AGGTCCATGG TGGAAAAGGG ACTAGGTTTA CCCTTAGTGA AGACATTTAT
    CCTGAGAAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90177
    Clone ID Related Accession (Same CDS sequence) XM_024994791.1 , XM_015463957.1
    Accession Version XM_015463957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGATGATCGG CACCCTCTTG AGAAAACATG GCAAGGGATT AGAAAAGGGC
    AGGGTGAAAT TGCCAAGCCA CTCAGATGGA ACCCAGATGA TCTTCAATGC AGCCAAGGAG
    CTGGGTCAGC TATCTAAACT CAAGGACCAC ATGGTACGAG AAGAAGCCAA GAGCTTGACC
    CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC CTGGATACTA TCAAGCAATA CTTCCATGCA
    GGTGGCGTGG GCCTCAAGAA AACTTTCCTG GAGAAGAGCC CAGACCTGCA GTCCCTGCGC
    TATGCCCTGT CCCTCTACAC GCAGGCCACC GACCTGCTCA TCAAGACATT CGTGCAGACG
    CAGTCGGCCC AGGTCCATGG TGGAAAAGGG ACTAGGTTTA CCCTTAGTGA AGACATTTAT
    CCTGAGAAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319443.1
    CDS157..5340
    Translation

    Target ORF information:

    RefSeq Version XM_015463957.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAACC CTGCATCCTC
    ATGAACAACA CTCAGCAGCT GAGAGTTCAG CTGGAGAAGA TGTTCGAAGC CATGGGAGGG
    AAGGAGCTGG ATGCCGAGGC TAGTGACATC TTGAAGGAGC TACAGGTGAA ACTCAACAAC
    GTCTTGGATG AGCTCAGCCG GGTGTTTGCT ACCAGCTTCC AGCCACACAT TGAAGACTGT
    GTCAAGCAGA TGGGTGACAT CCTTAGCCAG GTGAAAGGCA CAGGCAATGT GCCAGCCAGT
    GCCTGCAGCA GCGTGGCCCA GGACGCTGAC AATGTGCTAC AACCCATCAT GGACCTGCTG
    GATAGCAACC TGACTCTCTT TGCCAAAATC TGTGAGAAGA CGGTGCTGAA GCGGGTGCTG
    AAGGAGTTAT GGAAGCTGGT CATGAACACC ATGGAGAAGA CCATCGTCCT GCCACCCCTC
    ACTGACCAGA CGATGATCGG CACCCTCTTG AGAAAACATG GCAAGGGATT AGAAAAGGGC
    AGGGTGAAAT TGCCAAGCCA CTCAGATGGA ACCCAGATGA TCTTCAATGC AGCCAAGGAG
    CTGGGTCAGC TATCTAAACT CAAGGACCAC ATGGTACGAG AAGAAGCCAA GAGCTTGACC
    CCGAAGCAGT GTGCAGTTGT TGAGCTGGCC CTGGATACTA TCAAGCAATA CTTCCATGCA
    GGTGGCGTGG GCCTCAAGAA AACTTTCCTG GAGAAGAGCC CAGACCTGCA GTCCCTGCGC
    TATGCCCTGT CCCTCTACAC GCAGGCCACC GACCTGCTCA TCAAGACATT CGTGCAGACG
    CAGTCGGCCC AGGTCCATGG TGGAAAAGGG ACTAGGTTTA CCCTTAGTGA AGACATTTAT
    CCTGAGAAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90178
    Clone ID Related Accession (Same CDS sequence) XM_015463958.1 , XM_024994793.1
    Accession Version XM_015463958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGA TGATCGGCAC CCTCTTGAGA
    AAACATGGCA AGGGATTAGA AAAGGGCAGG GTGAAATTGC CAAGCCACTC AGATGGAACC
    CAGATGATCT TCAATGCAGC CAAGGAGCTG GGTCAGCTAT CTAAACTCAA GGACCACATG
    GTACGAGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG
    GATACTATCA AGCAATACTT CCATGCAGGT GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG
    AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT GCCCTGTCCC TCTACACGCA GGCCACCGAC
    CTGCTCATCA AGACATTCGT GCAGACGCAG TCGGCCCAGG TCCATGGTGG AAAAGGGACT
    AGGTTTACCC TTAGTGAAGA CATTTATCCT GAGAAGGGCT CGGGTGTGGA AGACCCTGTG
    GGTGAAGTCT CCGTCCACGT AGAGCTCTTC ACTCATCCAG GAACTGGGGA ACACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGATCTC AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA
    TTCATCGAGG TCAACATCAT TGGGCCCCAG CTTAGTGACA AGAAACGCAA GTTTGCGACC
    AAATCCAAGA ACAACAGCTG GGCCCCCAAG TACAATGAGA GCTTTCAGTT CACGCTGAGC
    GCTGACACAG GCCCTGAGTG CTACGAGCTG CAGGTGTGCG TCAAGGACTA CTGCTTCGCG
    CGCGAGGACC GCACGGTGGG GCTGGCCGTG CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG
    AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC CGCATCCACA TGGACGACAC GGGCCTCACG
    GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT GACGAGGTGG CCAAGGAATT CGTCAAGCTC
    AAGTCGGACA CGCGCTCCGC CGAGGAAGGC GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319444.1
    CDS157..5307
    Translation

    Target ORF information:

    RefSeq Version XM_015463958.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGA TGATCGGCAC CCTCTTGAGA
    AAACATGGCA AGGGATTAGA AAAGGGCAGG GTGAAATTGC CAAGCCACTC AGATGGAACC
    CAGATGATCT TCAATGCAGC CAAGGAGCTG GGTCAGCTAT CTAAACTCAA GGACCACATG
    GTACGAGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG
    GATACTATCA AGCAATACTT CCATGCAGGT GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG
    AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT GCCCTGTCCC TCTACACGCA GGCCACCGAC
    CTGCTCATCA AGACATTCGT GCAGACGCAG TCGGCCCAGG TCCATGGTGG AAAAGGGACT
    AGGTTTACCC TTAGTGAAGA CATTTATCCT GAGAAGGGCT CGGGTGTGGA AGACCCTGTG
    GGTGAAGTCT CCGTCCACGT AGAGCTCTTC ACTCATCCAG GAACTGGGGA ACACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGATCTC AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA
    TTCATCGAGG TCAACATCAT TGGGCCCCAG CTTAGTGACA AGAAACGCAA GTTTGCGACC
    AAATCCAAGA ACAACAGCTG GGCCCCCAAG TACAATGAGA GCTTTCAGTT CACGCTGAGC
    GCTGACACAG GCCCTGAGTG CTACGAGCTG CAGGTGTGCG TCAAGGACTA CTGCTTCGCG
    CGCGAGGACC GCACGGTGGG GCTGGCCGTG CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG
    AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC CGCATCCACA TGGACGACAC GGGCCTCACG
    GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT GACGAGGTGG CCAAGGAATT CGTCAAGCTC
    AAGTCGGACA CGCGCTCCGC CGAGGAAGGC GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90178
    Clone ID Related Accession (Same CDS sequence) XM_015463958.1 , XM_024994793.1
    Accession Version XM_024994793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGA TGATCGGCAC CCTCTTGAGA
    AAACATGGCA AGGGATTAGA AAAGGGCAGG GTGAAATTGC CAAGCCACTC AGATGGAACC
    CAGATGATCT TCAATGCAGC CAAGGAGCTG GGTCAGCTAT CTAAACTCAA GGACCACATG
    GTACGAGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG
    GATACTATCA AGCAATACTT CCATGCAGGT GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG
    AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT GCCCTGTCCC TCTACACGCA GGCCACCGAC
    CTGCTCATCA AGACATTCGT GCAGACGCAG TCGGCCCAGG TCCATGGTGG AAAAGGGACT
    AGGTTTACCC TTAGTGAAGA CATTTATCCT GAGAAGGGCT CGGGTGTGGA AGACCCTGTG
    GGTGAAGTCT CCGTCCACGT AGAGCTCTTC ACTCATCCAG GAACTGGGGA ACACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGATCTC AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA
    TTCATCGAGG TCAACATCAT TGGGCCCCAG CTTAGTGACA AGAAACGCAA GTTTGCGACC
    AAATCCAAGA ACAACAGCTG GGCCCCCAAG TACAATGAGA GCTTTCAGTT CACGCTGAGC
    GCTGACACAG GCCCTGAGTG CTACGAGCTG CAGGTGTGCG TCAAGGACTA CTGCTTCGCG
    CGCGAGGACC GCACGGTGGG GCTGGCCGTG CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG
    AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC CGCATCCACA TGGACGACAC GGGCCTCACG
    GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT GACGAGGTGG CCAAGGAATT CGTCAAGCTC
    AAGTCGGACA CGCGCTCCGC CGAGGAAGGC GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850561.1
    CDS150..5300
    Translation

    Target ORF information:

    RefSeq Version XM_024994793.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTATGATGA CCCTGACAGC
    GCAGTGGATG ACCGTGACAG TGACTACCGC AGTGAAACAA GCAACAGCAT CCCGCCACCC
    TATTACACTA CGTCCCAGCC CAATGCCTCA GTCCACCAGT ATTCTGTCCG CCCACCACCC
    CTGGGTTCCC GGGAATCCTA CAGCGACTCC ATGCACAGCT ATGAGGAGTT CTCCGAGCCG
    CGGGCCCTCA GCCCCACAGG CAGCAGCCGC TACGCCTCCT CAGGGGAGCT GAGCCAAGGC
    AGTTCACAGC TGAGCGAGGA CTTCGACCCG GATGAGCAGA GCCTGCAGGG CTCTGAGCTA
    GAAGATGAGC GGGACCGGGA CTCCTACCAC TCCTGCCACA GCTCGGTCAG CTACCACAAG
    GACTCGCCAC GCTGGGACCA GGACGAGGAC GAATTGGATG AGGAGCTGGA TGAAGAGCTG
    GATGAGGACC TGGAGGACTT CCTGGAGGAG GAGGAGGAGC TACCTGAAGA CGAGGAAGAG
    CTGGAGGAGG AGGAGGAGGT GCCAGATGAC ATCCGAGGCT ATGCCATGCG AGAGGAGGTG
    GCCGTGGCCG AGCCCAAGGA CTTCAAGCGT GTCAGCCTTC CACCTGCCGC TCCCGAGAAA
    GAGGAGCAGG CCCCAGCCAT GCCCACTGAG GCCCCCGACA TACCCAAGGT GGCCCCTGAG
    CCAGCCGTGC CTGAGGAAGT GCCTGCATCT GAGCAGGAGC CTGAGGCCCC CAAGGCTGAG
    GAGAGTTTCA GGCCCCGAGA AGATGAGGAA GGCCAAGAGG GTCAGGACTC CATGTCTAGA
    GCCAAGGCCA ACTGGCTGCG AGCCTTCAAC AAAGTGCGGC TGCAGCTGCA GGAGGCCCGG
    GGAGAAGGAG AAATGTCCAA GTCCCTGTGG TTCAAAGGCG GCCCTGGCGG TGGTCTCATC
    ATCATTGATA GCATGCCGGA CATCCGCAAG AGGAAGCCGA TCCCACTCGT GAGCGACCTG
    GCCATGTCCC TTGTCCAGTC CAGAAAAGCA GGCATCACCT CCGCCTTGGC ATCCAGCACT
    TTGAACAACG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC
    CCCATCTCGT GCACGACGCC ACACAACTTC GAGGTGTGGA CGGCTACCAC ACCCACCTAT
    TGCTACGAGT GTGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG CTGTACCGAG
    TGCGGTGTCA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCAGACTG TCTGCAGCGG
    GCGGCGGAGA AGAGCTCCAA GCACGGGGCG GAGGACCGGA CGCAGAACAT CATCATGGTG
    CTCAAGGACC GCATGAAGAT CCGGGAGCGC AACAAGCCCG AGATCTTCGA GCTCATCCAG
    GAGATCTTCG CGGTGACCAA GACTGCACAC ACGCAACAGA TGAAGGCAGT CAAGCAGAGC
    GTGCTGGACG GAACATCCAA GTGGTCGGCC AAGATCAGCA TCACTGTGGT CTGTGCCCAG
    GGGTTGCAGG CAAAGGACAA GACGGGATCC AGTGACCCCT ATGTCACCGT CCAGGTTGGG
    AAGACCAAGA AACGGACAAA AACCATCTAT GGAAACCTGA ACCCCGTGTG GGAGGAGAAC
    TTTCACTTTG AATGCCACAA CTCCTCAGAC CGAATCAAGG TGCGCGTCTG GGATGAGGAC
    GACGATATCA AATCTCGCGT GAAGCAGCGG TTCAAAAGGG AATCCGATGA CTTCCTGGGT
    CAGACGATCA TCGAGGTGCG GACACTCAGT GGCGAGATGG ACGTGTGGTA CAACCTGGAC
    AAGCGGACTG ACAAATCTGC TGTGTCAGGT GCCATCCGGC TGCACATCAG TGTGGAGATC
    AAAGGTGAAG AGAAGGTGGC TCCATACCAC GTCCAGTACA CCTGTTTGCA CGAGAACCTG
    TTCCACTTCG TGACAGACGT GCAGAACAAT GGGGTCGTGA AGATCCCAGA TGCCAAGGGT
    GATGACGCCT GGAAAGTTTA CTATGATGAG ACAGCCCAGG AGATTGTAGA TGAATTTGCC
    ATGCGCTACG GCGTCGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCGTCC
    AAGTACATGT GTCCTGGGGT GCCTGCCGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC
    TATTATGCTC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCCGCCTCC
    AACTTTGGGA AAGAGCGCTT TGTGAAGCTC CTGGACCAGC TGCATAACTC CCTGCGGATA
    GACCTCTCCA TGTATCGGAA TAACTTCCCA GCCAGCAGCC CAGAGAGACT CCAGGACCTC
    AAATCCACTG TGGACCTTCT CACCAGCATC ACCTTCTTTC GGATGAAGGT ACAAGAACTC
    CAGAGCCCAC CCCGAGCCAG CCAGGTGGTG AAGGACTGTG TGAAAGCCTG CCTCAACTCT
    ACTTATGAGT ACATCTTCAA CAATTGTCAT GAACTCTATA GTCGAGAGTA CCAGACTGAC
    CCGGCCAAGA AGGGGGAAGT TCCCCCAGAG GAACAAGGCC CCAGCATCAA GAACCTCGAC
    TTCTGGTCCA AGCTGATCAC TCTCATAGTG TCCATCATTG AGGAAGACAA AAATTCGTAC
    ACTCCCTGCC TCAACCAGTT TCCCCAGGAG TTGAACGTGG GTAAAATCAG CGCTGAAGTG
    ATGTGGAATC TGTTTGCCCA GGATATGAAG TATGCAATGG AGGAGCATGA TAAGCATCGC
    CTGTGCAAGA GTGCCGACTA CATGAACCTC CACTTCAAGG TCAAATGGCT CTACAATGAG
    TATGTGGCTG AGCTGCCTGC CTTTAAGGAC CGAGTGCCTG AGTATCCTGC GTGGTTTGAG
    CCCTTTGTCA TCCAGTGGCT GGACGAGAAT GAGGAAGTGT CCCGAGATTT CTTGCATGGA
    GCCCTGGAGA GAGACAAGAA GGATGGGTTC CAGCAGACTT CAGAGCATGC CCTGTTCTCC
    TGCTCTGTGG TGGATGTCTT CTCCCAGCTC AATCAGAGCT TTGAGATCAT CAAGAAACTC
    GAGTGTCCTG ACCCCCAGAT TGTGGGGCAC TACATGAGGC GCTTTGCCAA GACCATCAGT
    AATGTGCTCC TCCAGTATGC AGACATCATC TCCAAGGACT TCGCTTCCTA CTGCTCCAAG
    GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACTC AGCAGCTGAG AGTTCAGCTG
    GAGAAGATGT TCGAAGCCAT GGGAGGGAAG GAGCTGGATG CCGAGGCTAG TGACATCTTG
    AAGGAGCTAC AGGTGAAACT CAACAACGTC TTGGATGAGC TCAGCCGGGT GTTTGCTACC
    AGCTTCCAGC CACACATTGA AGACTGTGTC AAGCAGATGG GTGACATCCT TAGCCAGGTG
    AAAGGCACAG GCAATGTGCC AGCCAGTGCC TGCAGCAGCG TGGCCCAGGA CGCTGACAAT
    GTGCTACAAC CCATCATGGA CCTGCTGGAT AGCAACCTGA CTCTCTTTGC CAAAATCTGT
    GAGAAGACGG TGCTGAAGCG GGTGCTGAAG GAGTTATGGA AGCTGGTCAT GAACACCATG
    GAGAAGACCA TCGTCCTGCC ACCCCTCACT GACCAGACGA TGATCGGCAC CCTCTTGAGA
    AAACATGGCA AGGGATTAGA AAAGGGCAGG GTGAAATTGC CAAGCCACTC AGATGGAACC
    CAGATGATCT TCAATGCAGC CAAGGAGCTG GGTCAGCTAT CTAAACTCAA GGACCACATG
    GTACGAGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG
    GATACTATCA AGCAATACTT CCATGCAGGT GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG
    AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT GCCCTGTCCC TCTACACGCA GGCCACCGAC
    CTGCTCATCA AGACATTCGT GCAGACGCAG TCGGCCCAGG TCCATGGTGG AAAAGGGACT
    AGGTTTACCC TTAGTGAAGA CATTTATCCT GAGAAGGGCT CGGGTGTGGA AGACCCTGTG
    GGTGAAGTCT CCGTCCACGT AGAGCTCTTC ACTCATCCAG GAACTGGGGA ACACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGATCTC AAGTGGCAGA CTTCTGGCAT TTTCCGGCCA
    TTCATCGAGG TCAACATCAT TGGGCCCCAG CTTAGTGACA AGAAACGCAA GTTTGCGACC
    AAATCCAAGA ACAACAGCTG GGCCCCCAAG TACAATGAGA GCTTTCAGTT CACGCTGAGC
    GCTGACACAG GCCCTGAGTG CTACGAGCTG CAGGTGTGCG TCAAGGACTA CTGCTTCGCG
    CGCGAGGACC GCACGGTGGG GCTGGCCGTG CTGCAGCTAC GCGAGCTGGT CCAGCGCGGG
    AGCGCCGCCT GCTGGTTGCC CCTTGGCCGC CGCATCCACA TGGACGACAC GGGCCTCACG
    GTGCTGCGCA TCCTGTCGCA ACGCAGCAAT GACGAGGTGG CCAAGGAATT CGTCAAGCTC
    AAGTCGGACA CGCGCTCCGC CGAGGAAGGC GGTGCCGCGC CTGCCCCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90179
    Clone ID Related Accession (Same CDS sequence) XM_015463959.1 , XM_024994794.1
    Accession Version XM_015463959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGCTC GGGTGTGGAA GACCCTGTGG GTGAAGTCTC CGTCCACGTA
    GAGCTCTTCA CTCATCCAGG AACTGGGGAA CACAAGGTCA CAGTGAAAGT GGTGGCTGCC
    AATGATCTCA AGTGGCAGAC TTCTGGCATT TTCCGGCCAT TCATCGAGGT CAACATCATT
    GGGCCCCAGC TTAGTGACAA GAAACGCAAG TTTGCGACCA AATCCAAGAA CAACAGCTGG
    GCCCCCAAGT ACAATGAGAG CTTTCAGTTC ACGCTGAGCG CTGACACAGG CCCTGAGTGC
    TACGAGCTGC AGGTGTGCGT CAAGGACTAC TGCTTCGCGC GCGAGGACCG CACGGTGGGG
    CTGGCCGTGC TGCAGCTACG CGAGCTGGTC CAGCGCGGGA GCGCCGCCTG CTGGTTGCCC
    CTTGGCCGCC GCATCCACAT GGACGACACG GGCCTCACGG TGCTGCGCAT CCTGTCGCAA
    CGCAGCAATG ACGAGGTGGC CAAGGAATTC GTCAAGCTCA AGTCGGACAC GCGCTCCGCC
    GAGGAAGGCG GTGCCGCGCC TGCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319445.1
    CDS157..5286
    Translation

    Target ORF information:

    RefSeq Version XM_015463959.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGCTC GGGTGTGGAA GACCCTGTGG GTGAAGTCTC CGTCCACGTA
    GAGCTCTTCA CTCATCCAGG AACTGGGGAA CACAAGGTCA CAGTGAAAGT GGTGGCTGCC
    AATGATCTCA AGTGGCAGAC TTCTGGCATT TTCCGGCCAT TCATCGAGGT CAACATCATT
    GGGCCCCAGC TTAGTGACAA GAAACGCAAG TTTGCGACCA AATCCAAGAA CAACAGCTGG
    GCCCCCAAGT ACAATGAGAG CTTTCAGTTC ACGCTGAGCG CTGACACAGG CCCTGAGTGC
    TACGAGCTGC AGGTGTGCGT CAAGGACTAC TGCTTCGCGC GCGAGGACCG CACGGTGGGG
    CTGGCCGTGC TGCAGCTACG CGAGCTGGTC CAGCGCGGGA GCGCCGCCTG CTGGTTGCCC
    CTTGGCCGCC GCATCCACAT GGACGACACG GGCCTCACGG TGCTGCGCAT CCTGTCGCAA
    CGCAGCAATG ACGAGGTGGC CAAGGAATTC GTCAAGCTCA AGTCGGACAC GCGCTCCGCC
    GAGGAAGGCG GTGCCGCGCC TGCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90179
    Clone ID Related Accession (Same CDS sequence) XM_015463959.1 , XM_024994794.1
    Accession Version XM_024994794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGCTC GGGTGTGGAA GACCCTGTGG GTGAAGTCTC CGTCCACGTA
    GAGCTCTTCA CTCATCCAGG AACTGGGGAA CACAAGGTCA CAGTGAAAGT GGTGGCTGCC
    AATGATCTCA AGTGGCAGAC TTCTGGCATT TTCCGGCCAT TCATCGAGGT CAACATCATT
    GGGCCCCAGC TTAGTGACAA GAAACGCAAG TTTGCGACCA AATCCAAGAA CAACAGCTGG
    GCCCCCAAGT ACAATGAGAG CTTTCAGTTC ACGCTGAGCG CTGACACAGG CCCTGAGTGC
    TACGAGCTGC AGGTGTGCGT CAAGGACTAC TGCTTCGCGC GCGAGGACCG CACGGTGGGG
    CTGGCCGTGC TGCAGCTACG CGAGCTGGTC CAGCGCGGGA GCGCCGCCTG CTGGTTGCCC
    CTTGGCCGCC GCATCCACAT GGACGACACG GGCCTCACGG TGCTGCGCAT CCTGTCGCAA
    CGCAGCAATG ACGAGGTGGC CAAGGAATTC GTCAAGCTCA AGTCGGACAC GCGCTCCGCC
    GAGGAAGGCG GTGCCGCGCC TGCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850562.1
    CDS150..5279
    Translation

    Target ORF information:

    RefSeq Version XM_024994794.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGCTC GGGTGTGGAA GACCCTGTGG GTGAAGTCTC CGTCCACGTA
    GAGCTCTTCA CTCATCCAGG AACTGGGGAA CACAAGGTCA CAGTGAAAGT GGTGGCTGCC
    AATGATCTCA AGTGGCAGAC TTCTGGCATT TTCCGGCCAT TCATCGAGGT CAACATCATT
    GGGCCCCAGC TTAGTGACAA GAAACGCAAG TTTGCGACCA AATCCAAGAA CAACAGCTGG
    GCCCCCAAGT ACAATGAGAG CTTTCAGTTC ACGCTGAGCG CTGACACAGG CCCTGAGTGC
    TACGAGCTGC AGGTGTGCGT CAAGGACTAC TGCTTCGCGC GCGAGGACCG CACGGTGGGG
    CTGGCCGTGC TGCAGCTACG CGAGCTGGTC CAGCGCGGGA GCGCCGCCTG CTGGTTGCCC
    CTTGGCCGCC GCATCCACAT GGACGACACG GGCCTCACGG TGCTGCGCAT CCTGTCGCAA
    CGCAGCAATG ACGAGGTGGC CAAGGAATTC GTCAAGCTCA AGTCGGACAC GCGCTCCGCC
    GAGGAAGGCG GTGCCGCGCC TGCCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90180
    Clone ID Related Accession (Same CDS sequence) XM_024994795.1 , XM_015463960.1
    Accession Version XM_024994795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGAACCCAG ATGATCTTCA ATGCAGCCAA GGAGCTGGGT
    CAGCTATCTA AACTCAAGGA CCACATGGTA CGAGAAGAAG CCAAGAGCTT GACCCCGAAG
    CAGTGTGCAG TTGTTGAGCT GGCCCTGGAT ACTATCAAGC AATACTTCCA TGCAGGTGGC
    GTGGGCCTCA AGAAAACTTT CCTGGAGAAG AGCCCAGACC TGCAGTCCCT GCGCTATGCC
    CTGTCCCTCT ACACGCAGGC CACCGACCTG CTCATCAAGA CATTCGTGCA GACGCAGTCG
    GCCCAGGTCC ATGGTGGAAA AGGGACTAGG TTTACCCTTA GTGAAGACAT TTATCCTGAG
    AAGGGCTCGG GTGTGGAAGA CCCTGTGGGT GAAGTCTCCG TCCACGTAGA GCTCTTCACT
    CATCCAGGAA CTGGGGAACA CAAGGTCACA GTGAAAGTGG TGGCTGCCAA TGATCTCAAG
    TGGCAGACTT CTGGCATTTT CCGGCCATTC ATCGAGGTCA ACATCATTGG GCCCCAGCTT
    AGTGACAAGA AACGCAAGTT TGCGACCAAA TCCAAGAACA ACAGCTGGGC CCCCAAGTAC
    AATGAGAGCT TTCAGTTCAC GCTGAGCGCT GACACAGGCC CTGAGTGCTA CGAGCTGCAG
    GTGTGCGTCA AGGACTACTG CTTCGCGCGC GAGGACCGCA CGGTGGGGCT GGCCGTGCTG
    CAGCTACGCG AGCTGGTCCA GCGCGGGAGC GCCGCCTGCT GGTTGCCCCT TGGCCGCCGC
    ATCCACATGG ACGACACGGG CCTCACGGTG CTGCGCATCC TGTCGCAACG CAGCAATGAC
    GAGGTGGCCA AGGAATTCGT CAAGCTCAAG TCGGACACGC GCTCCGCCGA GGAAGGCGGT
    GCCGCGCCTG CCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850563.1
    CDS150..5267
    Translation

    Target ORF information:

    RefSeq Version XM_024994795.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGAACCCAG ATGATCTTCA ATGCAGCCAA GGAGCTGGGT
    CAGCTATCTA AACTCAAGGA CCACATGGTA CGAGAAGAAG CCAAGAGCTT GACCCCGAAG
    CAGTGTGCAG TTGTTGAGCT GGCCCTGGAT ACTATCAAGC AATACTTCCA TGCAGGTGGC
    GTGGGCCTCA AGAAAACTTT CCTGGAGAAG AGCCCAGACC TGCAGTCCCT GCGCTATGCC
    CTGTCCCTCT ACACGCAGGC CACCGACCTG CTCATCAAGA CATTCGTGCA GACGCAGTCG
    GCCCAGGTCC ATGGTGGAAA AGGGACTAGG TTTACCCTTA GTGAAGACAT TTATCCTGAG
    AAGGGCTCGG GTGTGGAAGA CCCTGTGGGT GAAGTCTCCG TCCACGTAGA GCTCTTCACT
    CATCCAGGAA CTGGGGAACA CAAGGTCACA GTGAAAGTGG TGGCTGCCAA TGATCTCAAG
    TGGCAGACTT CTGGCATTTT CCGGCCATTC ATCGAGGTCA ACATCATTGG GCCCCAGCTT
    AGTGACAAGA AACGCAAGTT TGCGACCAAA TCCAAGAACA ACAGCTGGGC CCCCAAGTAC
    AATGAGAGCT TTCAGTTCAC GCTGAGCGCT GACACAGGCC CTGAGTGCTA CGAGCTGCAG
    GTGTGCGTCA AGGACTACTG CTTCGCGCGC GAGGACCGCA CGGTGGGGCT GGCCGTGCTG
    CAGCTACGCG AGCTGGTCCA GCGCGGGAGC GCCGCCTGCT GGTTGCCCCT TGGCCGCCGC
    ATCCACATGG ACGACACGGG CCTCACGGTG CTGCGCATCC TGTCGCAACG CAGCAATGAC
    GAGGTGGCCA AGGAATTCGT CAAGCTCAAG TCGGACACGC GCTCCGCCGA GGAAGGCGGT
    GCCGCGCCTG CCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90180
    Clone ID Related Accession (Same CDS sequence) XM_024994795.1 , XM_015463960.1
    Accession Version XM_015463960.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGAACCCAG ATGATCTTCA ATGCAGCCAA GGAGCTGGGT
    CAGCTATCTA AACTCAAGGA CCACATGGTA CGAGAAGAAG CCAAGAGCTT GACCCCGAAG
    CAGTGTGCAG TTGTTGAGCT GGCCCTGGAT ACTATCAAGC AATACTTCCA TGCAGGTGGC
    GTGGGCCTCA AGAAAACTTT CCTGGAGAAG AGCCCAGACC TGCAGTCCCT GCGCTATGCC
    CTGTCCCTCT ACACGCAGGC CACCGACCTG CTCATCAAGA CATTCGTGCA GACGCAGTCG
    GCCCAGGTCC ATGGTGGAAA AGGGACTAGG TTTACCCTTA GTGAAGACAT TTATCCTGAG
    AAGGGCTCGG GTGTGGAAGA CCCTGTGGGT GAAGTCTCCG TCCACGTAGA GCTCTTCACT
    CATCCAGGAA CTGGGGAACA CAAGGTCACA GTGAAAGTGG TGGCTGCCAA TGATCTCAAG
    TGGCAGACTT CTGGCATTTT CCGGCCATTC ATCGAGGTCA ACATCATTGG GCCCCAGCTT
    AGTGACAAGA AACGCAAGTT TGCGACCAAA TCCAAGAACA ACAGCTGGGC CCCCAAGTAC
    AATGAGAGCT TTCAGTTCAC GCTGAGCGCT GACACAGGCC CTGAGTGCTA CGAGCTGCAG
    GTGTGCGTCA AGGACTACTG CTTCGCGCGC GAGGACCGCA CGGTGGGGCT GGCCGTGCTG
    CAGCTACGCG AGCTGGTCCA GCGCGGGAGC GCCGCCTGCT GGTTGCCCCT TGGCCGCCGC
    ATCCACATGG ACGACACGGG CCTCACGGTG CTGCGCATCC TGTCGCAACG CAGCAATGAC
    GAGGTGGCCA AGGAATTCGT CAAGCTCAAG TCGGACACGC GCTCCGCCGA GGAAGGCGGT
    GCCGCGCCTG CCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319446.1
    CDS157..5274
    Translation

    Target ORF information:

    RefSeq Version XM_015463960.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGAACCCAG ATGATCTTCA ATGCAGCCAA GGAGCTGGGT
    CAGCTATCTA AACTCAAGGA CCACATGGTA CGAGAAGAAG CCAAGAGCTT GACCCCGAAG
    CAGTGTGCAG TTGTTGAGCT GGCCCTGGAT ACTATCAAGC AATACTTCCA TGCAGGTGGC
    GTGGGCCTCA AGAAAACTTT CCTGGAGAAG AGCCCAGACC TGCAGTCCCT GCGCTATGCC
    CTGTCCCTCT ACACGCAGGC CACCGACCTG CTCATCAAGA CATTCGTGCA GACGCAGTCG
    GCCCAGGTCC ATGGTGGAAA AGGGACTAGG TTTACCCTTA GTGAAGACAT TTATCCTGAG
    AAGGGCTCGG GTGTGGAAGA CCCTGTGGGT GAAGTCTCCG TCCACGTAGA GCTCTTCACT
    CATCCAGGAA CTGGGGAACA CAAGGTCACA GTGAAAGTGG TGGCTGCCAA TGATCTCAAG
    TGGCAGACTT CTGGCATTTT CCGGCCATTC ATCGAGGTCA ACATCATTGG GCCCCAGCTT
    AGTGACAAGA AACGCAAGTT TGCGACCAAA TCCAAGAACA ACAGCTGGGC CCCCAAGTAC
    AATGAGAGCT TTCAGTTCAC GCTGAGCGCT GACACAGGCC CTGAGTGCTA CGAGCTGCAG
    GTGTGCGTCA AGGACTACTG CTTCGCGCGC GAGGACCGCA CGGTGGGGCT GGCCGTGCTG
    CAGCTACGCG AGCTGGTCCA GCGCGGGAGC GCCGCCTGCT GGTTGCCCCT TGGCCGCCGC
    ATCCACATGG ACGACACGGG CCTCACGGTG CTGCGCATCC TGTCGCAACG CAGCAATGAC
    GAGGTGGCCA AGGAATTCGT CAAGCTCAAG TCGGACACGC GCTCCGCCGA GGAAGGCGGT
    GCCGCGCCTG CCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90181
    Clone ID Related Accession (Same CDS sequence) XM_015463961.1 , XM_024994797.1
    Accession Version XM_015463961.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGTGC TGCTCTTAAC CCAGATCCCG GGTCCCCCAG ACAGGCGCTT
    GCCCATCTCA GATTCCAGCT GTGCCACTCC GTGGGTGGGC GCCTCCCGCA ACCGGGTGCC
    TCCTTGAGCC TCAGTTTTCT CATCTGTAAA ATGAGAACAG TCAAGGACCC TAATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319447.1
    CDS157..4896
    Translation

    Target ORF information:

    RefSeq Version XM_015463961.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463961.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGTGC TGCTCTTAAC CCAGATCCCG GGTCCCCCAG ACAGGCGCTT
    GCCCATCTCA GATTCCAGCT GTGCCACTCC GTGGGTGGGC GCCTCCCGCA ACCGGGTGCC
    TCCTTGAGCC TCAGTTTTCT CATCTGTAAA ATGAGAACAG TCAAGGACCC TAATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90181
    Clone ID Related Accession (Same CDS sequence) XM_015463961.1 , XM_024994797.1
    Accession Version XM_024994797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGTGC TGCTCTTAAC CCAGATCCCG GGTCCCCCAG ACAGGCGCTT
    GCCCATCTCA GATTCCAGCT GTGCCACTCC GTGGGTGGGC GCCTCCCGCA ACCGGGTGCC
    TCCTTGAGCC TCAGTTTTCT CATCTGTAAA ATGAGAACAG TCAAGGACCC TAATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850565.1
    CDS151..4890
    Translation

    Target ORF information:

    RefSeq Version XM_024994797.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (UNC13A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGATGAT CGGCACCCTC TTGAGAAAAC ATGGCAAGGG ATTAGAAAAG
    GGCAGGGTGA AATTGCCAAG CCACTCAGAT GGAACCCAGA TGATCTTCAA TGCAGCCAAG
    GAGCTGGGTC AGCTATCTAA ACTCAAGGAC CACATGGTAC GAGAAGAAGC CAAGAGCTTG
    ACCCCGAAGC AGTGTGCAGT TGTTGAGCTG GCCCTGGATA CTATCAAGCA ATACTTCCAT
    GCAGGTGGCG TGGGCCTCAA GAAAACTTTC CTGGAGAAGA GCCCAGACCT GCAGTCCCTG
    CGCTATGCCC TGTCCCTCTA CACGCAGGCC ACCGACCTGC TCATCAAGAC ATTCGTGCAG
    ACGCAGTCGG CCCAGGGTGC TGCTCTTAAC CCAGATCCCG GGTCCCCCAG ACAGGCGCTT
    GCCCATCTCA GATTCCAGCT GTGCCACTCC GTGGGTGGGC GCCTCCCGCA ACCGGGTGCC
    TCCTTGAGCC TCAGTTTTCT CATCTGTAAA ATGAGAACAG TCAAGGACCC TAATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05033
    Clone ID Related Accession (Same CDS sequence) XM_002688573.3
    Accession Version XM_002688573.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002688573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGCAC CCTCTTGAGA AAACATGGCA AGGGATTAGA AAAGGGCAGG
    GTGAAATTGC CAAGCCACTC AGATGGAACC CAGATGATCT TCAATGCAGC CAAGGAGCTG
    GGTCAGCTAT CTAAACTCAA GGACCACATG GTACGAGAAG AAGCCAAGAG CTTGACCCCG
    AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG GATACTATCA AGCAATACTT CCATGCAGGT
    GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT
    GCCCTGTCCC TCTACACGCA GGCCACCGAC CTGCTCATCA AGACATTCGT GCAGACGCAG
    TCGGCCCAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002688619.2
    CDS157..5280
    Translation

    Target ORF information:

    RefSeq Version XM_002688573.3
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog A (C. elegans) (UNC13A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688573.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTCTGC TTTGCGTTGG AGTCAAAAAA GCGAAGTTTG ATGGTGCCCA AGAGAAATTC 
    AACACCTACG TGACCCTGAA GGTGCAGAAT GTCAAAAGCA CGACTATCGC TGTCCGAGGC
    AGCCAGCCCA GCTGGGAGCA GGATTTCATG TTTGAGATTA ATCGCCTGGA TTTGGGGCTG
    ACAGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGCAC TGTGTGGATC
    CCCCTGCGGA CCATCCGCCA GTCCAATGAA GAGGGTCCTG GAGAGTGGCT GACCCTGGAC
    TCTCAGGTCA TCATGGCAGA CAGCGAGATC TGCGGTACTA AGGACCCCAC CTTCCACCGC
    ATCCTCCTCG ACACCCGCTT TGAGCTGCCC TTAGACATCC CAGAAGAGGA GGCCCGTTAC
    TGGGCCAAGA AGCTGGAGCA GCTGAACGCC ATGCGGGACC AGGATGAGTA TTCATTCCAG
    GATGAGCAAG ACAAGCCTCT TCCTGTTCCC AGCAATCAGT GTTGCAACTG GAATTATTTT
    GGCTGGGGTG AGCAGCAGAA TGATGACCCT GACAGCGCAG TGGATGACCG TGACAGTGAC
    TACCGCAGTG AAACAAGCAA CAGCATCCCG CCACCCTATT ACACTACGTC CCAGCCCAAT
    GCCTCAGTCC ACCAGTATTC TGTCCGCCCA CCACCCCTGG GTTCCCGGGA ATCCTACAGC
    GACTCCATGC ACAGCTATGA GGAGTTCTCC GAGCCGCGGG CCCTCAGCCC CACAGGCAGC
    AGCCGCTACG CCTCCTCAGG GGAGCTGAGC CAAGGCAGTT CACAGCTGAG CGAGGACTTC
    GACCCGGATG AGCAGAGCCT GCAGGGCTCT GAGCTAGAAG ATGAGCGGGA CCGGGACTCC
    TACCACTCCT GCCACAGCTC GGTCAGCTAC CACAAGGACT CGCCACGCTG GGACCAGGAC
    GAGGACGAAT TGGATGAGGA GCTGGATGAA GAGCTGGATG AGGACCTGGA GGACTTCCTG
    GAGGAGGAGG AGGAGCTACC TGAAGACGAG GAAGAGCTGG AGGAGGAGGA GGAGGTGCCA
    GATGACATCC GAGGCTATGC CATGCGAGAG GAGGTGGCCG TGGCCGAGCC CAAGGACTTC
    AAGCGTGTCA GCCTTCCACC TGCCGCTCCC GAGAAAGAGG AGCAGGCCCC AGCCATGCCC
    ACTGAGGCCC CCGACATACC CAAGGTGGCC CCTGAGCCAG CCGTGCCTGA GGAAGTGCCT
    GCATCTGAGC AGGAGCCTGA GGCCCCCAAG GCTGAGGAGA GTTTCAGGCC CCGAGAAGAT
    GAGGAAGGCC AAGAGGGTCA GGACTCCATG TCTAGAGCCA AGGCCAACTG GCTGCGAGCC
    TTCAACAAAG TGCGGCTGCA GCTGCAGGAG GCCCGGGGAG AAGGAGAAAT GTCCAAGTCC
    CTGTGGTTCA AAGGCGGCCC TGGCGGTGGT CTCATCATCA TTGATAGCAT GCCGGACATC
    CGCAAGAGGA AGCCGATCCC ACTCGTGAGC GACCTGGCCA TGTCCCTTGT CCAGTCCAGA
    AAAGCAGGCA TCACCTCCGC CTTGGCATCC AGCACTTTGA ACAACGAAGA GCTGAAAAAT
    CACGTTTACA AGAAGACCCT GCAAGCCTTA ATCTACCCCA TCTCGTGCAC GACGCCACAC
    AACTTCGAGG TGTGGACGGC TACCACACCC ACCTATTGCT ACGAGTGTGA GGGGCTGCTG
    TGGGGCATCG CGCGGCAGGG CATGCGCTGT ACCGAGTGCG GTGTCAAGTG CCACGAGAAG
    TGCCAGGACC TGCTCAATGC AGACTGTCTG CAGCGGGCGG CGGAGAAGAG CTCCAAGCAC
    GGGGCGGAGG ACCGGACGCA GAACATCATC ATGGTGCTCA AGGACCGCAT GAAGATCCGG
    GAGCGCAACA AGCCCGAGAT CTTCGAGCTC ATCCAGGAGA TCTTCGCGGT GACCAAGACT
    GCACACACGC AACAGATGAA GGCAGTCAAG CAGAGCGTGC TGGACGGAAC ATCCAAGTGG
    TCGGCCAAGA TCAGCATCAC TGTGGTCTGT GCCCAGGGGT TGCAGGCAAA GGACAAGACG
    GGATCCAGTG ACCCCTATGT CACCGTCCAG GTTGGGAAGA CCAAGAAACG GACAAAAACC
    ATCTATGGAA ACCTGAACCC CGTGTGGGAG GAGAACTTTC ACTTTGAATG CCACAACTCC
    TCAGACCGAA TCAAGGTGCG CGTCTGGGAT GAGGACGACG ATATCAAATC TCGCGTGAAG
    CAGCGGTTCA AAAGGGAATC CGATGACTTC CTGGGTCAGA CGATCATCGA GGTGCGGACA
    CTCAGTGGCG AGATGGACGT GTGGTACAAC CTGGACAAGC GGACTGACAA ATCTGCTGTG
    TCAGGTGCCA TCCGGCTGCA CATCAGTGTG GAGATCAAAG GTGAAGAGAA GGTGGCTCCA
    TACCACGTCC AGTACACCTG TTTGCACGAG AACCTGTTCC ACTTCGTGAC AGACGTGCAG
    AACAATGGGG TCGTGAAGAT CCCAGATGCC AAGGGTGATG ACGCCTGGAA AGTTTACTAT
    GATGAGACAG CCCAGGAGAT TGTAGATGAA TTTGCCATGC GCTACGGCGT CGAGTCCATC
    TACCAAGCCA TGACCCACTT TGCCTGCCTC TCGTCCAAGT ACATGTGTCC TGGGGTGCCT
    GCCGTCATGA GCACCCTGCT TGCCAACATC AACGCCTATT ATGCTCACAC CACCGCCTCC
    ACCAACGTGT CTGCCTCTGA CCGCTTCGCC GCCTCCAACT TTGGGAAAGA GCGCTTTGTG
    AAGCTCCTGG ACCAGCTGCA TAACTCCCTG CGGATAGACC TCTCCATGTA TCGGAATAAC
    TTCCCAGCCA GCAGCCCAGA GAGACTCCAG GACCTCAAAT CCACTGTGGA CCTTCTCACC
    AGCATCACCT TCTTTCGGAT GAAGGTACAA GAACTCCAGA GCCCACCCCG AGCCAGCCAG
    GTGGTGAAGG ACTGTGTGAA AGCCTGCCTC AACTCTACTT ATGAGTACAT CTTCAACAAT
    TGTCATGAAC TCTATAGTCG AGAGTACCAG ACTGACCCGG CCAAGAAGGG GGAAGTTCCC
    CCAGAGGAAC AAGGCCCCAG CATCAAGAAC CTCGACTTCT GGTCCAAGCT GATCACTCTC
    ATAGTGTCCA TCATTGAGGA AGACAAAAAT TCGTACACTC CCTGCCTCAA CCAGTTTCCC
    CAGGAGTTGA ACGTGGGTAA AATCAGCGCT GAAGTGATGT GGAATCTGTT TGCCCAGGAT
    ATGAAGTATG CAATGGAGGA GCATGATAAG CATCGCCTGT GCAAGAGTGC CGACTACATG
    AACCTCCACT TCAAGGTCAA ATGGCTCTAC AATGAGTATG TGGCTGAGCT GCCTGCCTTT
    AAGGACCGAG TGCCTGAGTA TCCTGCGTGG TTTGAGCCCT TTGTCATCCA GTGGCTGGAC
    GAGAATGAGG AAGTGTCCCG AGATTTCTTG CATGGAGCCC TGGAGAGAGA CAAGAAGGAT
    GGGTTCCAGC AGACTTCAGA GCATGCCCTG TTCTCCTGCT CTGTGGTGGA TGTCTTCTCC
    CAGCTCAATC AGAGCTTTGA GATCATCAAG AAACTCGAGT GTCCTGACCC CCAGATTGTG
    GGGCACTACA TGAGGCGCTT TGCCAAGACC ATCAGTAATG TGCTCCTCCA GTATGCAGAC
    ATCATCTCCA AGGACTTCGC TTCCTACTGC TCCAAGGAGA AGGAGAAAGT GCCCTGCATC
    CTCATGAACA ACACTCAGCA GCTGAGAGTT CAGCTGGAGA AGATGTTCGA AGCCATGGGA
    GGGAAGGAGC TGGATGCCGA GGCTAGTGAC ATCTTGAAGG AGCTACAGGT GAAACTCAAC
    AACGTCTTGG ATGAGCTCAG CCGGGTGTTT GCTACCAGCT TCCAGCCACA CATTGAAGAC
    TGTGTCAAGC AGATGGGTGA CATCCTTAGC CAGGTGAAAG GCACAGGCAA TGTGCCAGCC
    AGTGCCTGCA GCAGCGTGGC CCAGGACGCT GACAATGTGC TACAACCCAT CATGGACCTG
    CTGGATAGCA ACCTGACTCT CTTTGCCAAA ATCTGTGAGA AGACGGTGCT GAAGCGGGTG
    CTGAAGGAGT TATGGAAGCT GGTCATGAAC ACCATGGAGA AGACCATCGT CCTGCCACCC
    CTCACTGACC AGACGGGCAC CCTCTTGAGA AAACATGGCA AGGGATTAGA AAAGGGCAGG
    GTGAAATTGC CAAGCCACTC AGATGGAACC CAGATGATCT TCAATGCAGC CAAGGAGCTG
    GGTCAGCTAT CTAAACTCAA GGACCACATG GTACGAGAAG AAGCCAAGAG CTTGACCCCG
    AAGCAGTGTG CAGTTGTTGA GCTGGCCCTG GATACTATCA AGCAATACTT CCATGCAGGT
    GGCGTGGGCC TCAAGAAAAC TTTCCTGGAG AAGAGCCCAG ACCTGCAGTC CCTGCGCTAT
    GCCCTGTCCC TCTACACGCA GGCCACCGAC CTGCTCATCA AGACATTCGT GCAGACGCAG
    TCGGCCCAGG GCTCGGGTGT GGAAGACCCT GTGGGTGAAG TCTCCGTCCA CGTAGAGCTC
    TTCACTCATC CAGGAACTGG GGAACACAAG GTCACAGTGA AAGTGGTGGC TGCCAATGAT
    CTCAAGTGGC AGACTTCTGG CATTTTCCGG CCATTCATCG AGGTCAACAT CATTGGGCCC
    CAGCTTAGTG ACAAGAAACG CAAGTTTGCG ACCAAATCCA AGAACAACAG CTGGGCCCCC
    AAGTACAATG AGAGCTTTCA GTTCACGCTG AGCGCTGACA CAGGCCCTGA GTGCTACGAG
    CTGCAGGTGT GCGTCAAGGA CTACTGCTTC GCGCGCGAGG ACCGCACGGT GGGGCTGGCC
    GTGCTGCAGC TACGCGAGCT GGTCCAGCGC GGGAGCGCCG CCTGCTGGTT GCCCCTTGGC
    CGCCGCATCC ACATGGACGA CACGGGCCTC ACGGTGCTGC GCATCCTGTC GCAACGCAGC
    AATGACGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC CGCCGAGGAA
    GGCGGTGCCG CGCCTGCCCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.