APC2 cDNA ORF clone, Bos taurus(cattle)

The following APC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236654 XM_002689175.6
Latest version!
Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa236655 XM_005209327.4
Latest version!
Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa236656 XM_005209328.4
Latest version!
Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa21449 XM_604945.9
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa21449 XM_005209328.3
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51193 XM_002689175.5
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51193 XM_005199027.3
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51194 XM_005199028.3
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51194 XM_005209327.3
Latest version!
Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236654
    Clone ID Related Accession (Same CDS sequence) XM_002689175.6
    Accession Version XM_002689175.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689175.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTATGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CGACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CTCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689221.5
    CDS109..6942
    Translation

    Target ORF information:

    RefSeq Version XM_002689175.6
    Organism Bos taurus(cattle)
    Definition Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689175.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTATGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CGACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CTCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236655
    Clone ID Related Accession (Same CDS sequence) XM_005209327.4
    Accession Version XM_005209327.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209327.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTATGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCGA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCTC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209384.3
    CDS109..6939
    Translation

    Target ORF information:

    RefSeq Version XM_005209327.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209327.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTATGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCGA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCTC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236656
    Clone ID Related Accession (Same CDS sequence) XM_005209328.4
    Accession Version XM_005209328.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209328.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTATGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCG ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCT CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209385.2
    CDS239..7000
    Translation

    Target ORF information:

    RefSeq Version XM_005209328.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC2, WNT signaling pathway regulator (APC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209328.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTATGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCG ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCT CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21449
    Clone ID Related Accession (Same CDS sequence) XM_604945.9 , XM_005209328.3
    Accession Version XM_604945.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_604945.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTACGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCC ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCC CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_604945.5
    CDS247..7008
    Translation

    Target ORF information:

    RefSeq Version XM_604945.9
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_604945.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTACGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCC ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCC CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21449
    Clone ID Related Accession (Same CDS sequence) XM_604945.9 , XM_005209328.3
    Accession Version XM_005209328.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTACGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCC ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCC CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209385.1
    CDS247..7008
    Translation

    Target ORF information:

    RefSeq Version XM_005209328.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209328.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACGAGCT CAGTGGCAGC CTATGAGCAG CTGGTGCGGC AGGTGGAGGC CTTGAAGGCC 
    GAGAACAGTC ACCTGCGGCA GGAGCTTCGG GATAATTCAA GCCACCTGTC CAAGCTGGAG
    ACAGAGACGT CGGGAATGAA GGCGGTCCTG CAGCACTTAC AGGGCAAGCT AGAGCAGGAG
    GCCCGTGTGT TGGTGTCCTC CGGGCAGACT GAGGTGCTGG AACAGCTGAA AGCCCTGCAG
    ATGGACATCA CCAGCCTGTA TAACCTCAAG TTCCAGCCCC CAGCTCTGGC CCCTGAACCC
    ACTACCAGGA CCCCTGAGGG AAGCCCGGTG CACAGCTCTG GGCCCTCCAA GGAGAATTTT
    GGGGAGCTGA GCCGGGCCAC CATTCGGCTC CTAGAGGAAC TGGACCGGGA ACGGTGTTTC
    CTGTTGAATG AGATTGAGAA GGAGGAAAAG GAGAAGCTCT GGTATTACTC GCAGTTGCAG
    GGGCTATCTA AGCGCTTGGA TGAACTCCCG CACGTGGAGA CGCAGTTCTC AATGCAGATG
    GATCTGATCC GGCAGCAGCT GGAATTCGAG GCCCAACACA TCCGCTCGCT GATGGAGGAG
    CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCGCAGA TCCGTGCTTC CCGTCTGGAG
    CAGATCGACA AGGAACTGCT GTCCGCGCAG GACCGAGTGC AGCAGACCGA GCCCCAGGCC
    TTGCTGGCAG TGAAGTCGAT GCCCATGGAC GAGGACGCTG AGAATGAGGT CCCCACGCAC
    CCTGAGGATG GTGCCCCTCA GCCAGGCAAC AGTAAGGTGG AGGTGGTCTT CTGGCTCCTG
    TCCATGCTGG CAACGCGGGA CCAGGAGGAC ACGGCGCGGA CGCTGCTCGC CATGTCCAGC
    TCACCCGAAA GCTGCGTGGC CATGCGCCGC TCAGGCTGTC TGCCGCTGCT GCTGCAGATC
    CTGCACGGCA CTGAGGCCGG GGCCGGGGGT CGCAACGGGA CCCCCGGGGC TCCGGGAGCC
    AAGGACGCGC GCATGCGTGC CAACGCAGCG CTGCACAATA TCGTCTTCTC TCAGCCCGAC
    CAGGGGCTAG CACGCAAGGA GATGCGCGTG TTACACGTGC TGGAGCAGAT CCGCGCCTAC
    TGCGAGACCT GTTGGGACTG GCTGCAGGCC CGGGATGGCG GGCCTGAGGG CAGCGGCACT
    GGTGCTGCGC CGGTGCCCAT CGAGCCACAG ATCTGTCAGG CCACCTGCGC CGTGATGAAG
    CTGTCCTTTG ACGAGGAATA CCGCCGAGCT ATGAATGAGC TGGGTGGGCT GCAGGCAGTG
    GCGGAGTTAC TGCAGGTTGA CCATGAGATG CACAAGATGA CCCGGGACCC TCTCAACCTG
    GCCCTGCGCC GATACGCTGG CATGACCCTC ACCAACCTCA CCTTCGGGGA CGTTGCCAAC
    AAGGCTGCAC TGTGCGCCCG CCGGGGCTGC ATGGAGGCCA TTGTGGCCCA GCTGGCATCT
    GAGAGTGAGG AGCTGCACCA GGTTGTGTCT AGCATCCTGC GCAACCTGTC CTGGAGGGCC
    GACATCAACA GCAAGAAGGT GCTGAGGGAA GTGGGCAGCA TGACTGCCCT GATGCAGTGC
    GTCCTGCGAG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TTAGCGCCCT GTGGAACCTC
    TCGGCGCACA GCACGGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGTGC CCTGGGCTTC
    CTGGTAAGCA CACTGACTTA CAAGTGCCAG AGCAACTCGC TAGCCATCAT TGAGAGCGGT
    GGTGGCATCC TGCGCAATGT GTCCAGCCTC ATCGCCACCC GCGAGGACTA CAGGCAGGTG
    CTGCGAGACC ACAATTGCCT GCAGACGCTG CTGCAGCACC TGACGTCGCA CAGCCTGACC
    ATAGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCCC CCGCGACCAG
    GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGGA ACCTGGTGCA CTCCAAGCAC
    AAAATGATCG CCATGGGCAG CGCCGCCGCG CTGCGCAACC TGCTGGCTCA CCGGCCTGCC
    AAGTACCAGC CAGCGGCCAC CGCGGTCTCC CCGGGCTCCT GCGCGCCCAG CCTGTACGTG
    CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACACACGGC ACCTGGCTCA GGCACTTGAC
    CACCTGGAGA AGCAGGGCCT GCCGGAGGCC GAGACCGCCT GCAAGAAGCC CCTCCCGCCC
    CTGCGCCACC TGGACGGCCT GGCCCAGGAC TACGCCTCTG ACTCGGGCTG TTTTGACGAC
    GACGATGCGC CCTCCCTGGC TGCTGCCGCC ACTGCCGCAG AGCCCGCCAG CCCCGCAGTG
    CTCTCCCTCT TCCTGGGCAG CCCCTTCCTG CAAGGCCAGG CGCTGGCCCG AGCCCCGCCC
    ACCCGCCGAG GAGGCCTGGA GGTGGAGAAG GAGGCCGGTG GAGAGGCAGC CGTGGCAGCT
    AGGGCCAAGG CCAAGCTGGC GCTGGCGGTG GCACGCATCG ACAGGCTGGT AGAGGACATC
    TCGGCCCTGC ACACCTCATC TGACGACAGC TTCAGCCTCA GCTCCGGGGA CCCTGGCCAG
    GAGGCCCCAC GAGAAGGCCG TGCCCAATCC TGCTCACCTT GCAGGGGGCC TGAGGGGGGG
    CGGCGGGAAG CAGGCAGCAG CCGGGCTCAC CCACTGCTGC GGCTCAAGGC AGCCCACGCC
    AGCCTTTCCA ATGACAGTCT CAACAGCGGT AGCACCAGTG ATGGGCACTG TCCTCGCGAA
    CACTCTCGGC CCTGCTCGCT GGCTGCTCTG GCCGAGCACC GAGAGGGGCC CCCACGTGGT
    CAGGCGCGGC CCAGCCGGCT TGACCTCAAC CTGCCTGGAG GCCAGGCTGA GCCTGAGGCC
    CGGGATGCCA AGGCCACAGA TGCCCACGTG CGTACCATCA AGTTGTCGCC CACCTACCAG
    CACGTGCCGC TGCTGGAGGG CACCACTAGG GCGGGTGCGG GGTCCCTGGC CCCTGGGGCC
    CGGAAACAGG CCTGGCTGTC CACAGAAGGC CTGAGCAGTG TGCCTGAGAA GCTAGTGGTT
    GAGAAGGCAC CCCTCTGCCT GTCCCGCTGC AGCTCGCTGT CCTCACTGTC CTCAGCTGGC
    CGCCCGGGGC CCAGTGAGGC TGGGGACCTA GATGAGAGCG ACTCATCCCT GGAGGGGCTG
    GAGGAGTCTG GCCCTGGTGA AACAGAGCTG GATGGGGCCT GGCGGGGCCC AGGGGCCGCC
    TCCCTGCCCA TGGCCATCCC AGCGCCCCAG AGGGGTCGGG GCCTGGGGGT GGAGGATGCC
    ACGCCGTCCA GCTCCTCTGA GAACTGCGTG CAGGAGACAC CGCTGGTGCT GAGCCGCTGC
    AGCTCAGTCA GTTCACTGGG CAGCTTCGAG AGCCCATCCA TTGCCAGCTC CATCCCCAGT
    GACCCATGCA GCGGGCTGGG CAGTGGCACG GTCAGCCCCA GTGAGCTGCC TGATAGCCCC
    GGGCAAACCA TGCCTCCGAG CCGCAGCAAG ACACCACCGC TGGCCCCAGC ACCACCGGGC
    GAGCGGGAAG CCACCCAGTT CAGCCTGCAG TGGGAGAGTT ACGTGAAGCG CTTCCTGGAC
    ATCGCCGACT GCCGGGAGCG CTGCCGGCTG CCCTCCGAGC TGGACGCAGG CAGCGTGCGT
    TTCACTGTGG AGAAGCCCGA CGAGAACTTC TCGTGTGCCT CCAGCCTCAG CGCACTGGCC
    CTGCATGAGC TCTACGTGCA GAAAGACGTG GAGCTGCGGC TGCTGCCCCC TGCCTGCCCA
    GAGCGCGGCA GTGGGGGAGG CGGAGGCCCA GGGCACCGCC GACGGGATGA GGCCAGTGGC
    TGTCTGGAAG GGCCGGCGTC CACCGACCAG GAGCTGGAGC TGCTGCGTGA GTGCCTGGGT
    GGGGCCGTGC CTGCCCGGCT CCGAAAGGTG GCCTCAGCAC TGGTGCCTGG CTGCCGCACG
    CTGCCCGTGC CAGTCTACAT GCTGGTGCCT GCCCCGGCCC GTGAGGACGA CTCCTGCACT
    GACTCGGCTG AGGGCACTCC AGTCAACTTC TCCAGCACCG CTTCCCTCAG CGACGAGACC
    CTGCAGGGAC CCCCCAAGGA TGGTGGGCGT TTGGATGGGA AGAAGCCCGT GGGTCGTGCT
    GCCTCCACTA GGCAGTCTGC TGGGCAGCGG CACAGGGTCG GGGGCATGGG CCGGAGCACA
    GAGCAGCCCC GGGGGGCTGG CAGTGGCAGG GCAGGGCTGG AGCTGCCCCT CCGGCGGCCC
    CCGAGCACCT GCAGGGACAC TGACAGCTCC CGCCCAGGCC AGGAGCACGG GGATGGGGCT
    CTGCAGTCTC TCTGCCTCAC AACGCCCACC GAGGAGGCTG TGTATTGTTT CTATGGCAAT
    GACTCGGATG AGGAGCCGGC CACAGCAGTG GCAGCAGCAC CCCCACGGCG GGCATCTGCG
    ATCCCCCGGG CGGTCAAGAG GGAGCGCCCC ACTGGTGCCA GGAAGGAGGT GCAGGCTGTG
    CCCAAGGCTG CACCCAAGGC GGCACCGCCT GCCCGGGCCC AGCCCAGCCT CATTGCAGAT
    GAGACACCAC CGTGCTACTC CCTGAGCTCC TCCGCCAGCT CCCTCAGCGA ACCTGAGCCC
    TCCGAGTGCA TGGCCAGTCG GCCCCGGGTC CACAAGCCAG GGCTCACCAA GGACCTGGTC
    CCTGGGGGCA GGAAGGATGG CTGCCCCAGC CCTCGGGCTG AGGCAGAGCT GCTCCGGCGT
    TGCATAGGCT CAGCCTTGCC CAGGCGCCAG CCCCAGGTGT CCGGCCCAAG GCGCCGCCCA
    CCCCGAGAGG CCCAGCCAAA GGCGCGTGCA GCAGAAGGGC CCCGGGAGCG CGGTGAGGAG
    GCGGCCAGCT CAGACCATGC CTCAGACCTG GACAGCATCG AGTGGCGTGC CATCCAGGAG
    GGTGCCAACT CTGTTGCCAC ATGGCTGCAC CAGGCCGCAG CGGCGGCTGC ACGTGAGGCC
    TCCTCAGAGT CTGATTCTGT TCTGTCCTTT GCAACAGGGC AGTCTGTGGG TTCCACCCTG
    CAGCCCACTG TGCATAGGAA GGGGTGTCGG CCAAGGGCAG AGCGCCAAGC GGGTGGTGCC
    CTGCGGCCAG AGAAACCAGA AGGGGCCCTC ACCCAGCGCA GCAGCGGGCC GGAGAAGCCG
    CGTGGCACTC AGAAGGCCCC AAATGGGGTG CCGGCGGTGC TCCGGGGACA TACAGTGATC
    TACATGCCCA CCCCGGCCAC CCGGGCACAG CCCAGAGGTG CCCCTGGTTC CCGCATTGTG
    GCAAGAAAGA TGGGAGCGCC CAGCCCCGTG CAGCCGGCAG CCCCCACCAA AGCCCCCAAC
    CCAGGGCAGC AGCGGTCTAG GAGCCTGCAC CGGCCTGGCA AGATCTCAGA GCTGGCAACA
    CTCAGCCCCC CGCAGAGAAG TGCCACACCA CCAGCCCGCC TTGCCAAGAC CCCATCGTCA
    AGTTCCTCTC AGACCTCACC GGCCTCCCAG CCTCTGCCCA GGAGGTCACC CCCTGCTGCC
    CAGCCTGCAG GGCCCCTGCC CGGCCCCAGG GCCTCCCCAG TGCCCAAGAC TCCAGCAAGG
    GCCCTGCTGG CCAAGCAGCA CAAGACGCAG AAGTCACCCG TGAGGATCCC CTTCATGCAG
    AGGCCTGCCA GGCGGGGGCC GCCGCCCTTG GCTAGGGCAG CCCCGGAGCC AGGCCCCAGG
    GGCCGGGTGG GCGCAGAAGG CGGCTCTGCA GCCCGAGGGG GCCGCCTGGG CCTGGTGCGC
    GTGGCCTCCG CCCGCTCCAG CGGCAGCGAA TCCTCCGACC GCTCGGGTTT CCGGCGGCAG
    CTGACCTTTA TCAAGGAGTC GCCGGGCCTG ATGCGGCGCC GACGCTCGGA ACTGTCCGCA
    CCCGAAGCCA CCAACCCGAC CACCCAGGCT GCCTCGCCCT GCCGCGGCCG GCCAGCACTA
    CCCGCTGTCT TCCTCTGCTC CTCGCGCTGT GATGAGCTGC GGGCAGGCTC CCGGCAGGCC
    CCGGCCCCGC AGCGGGCCCA CACGGCCCGG CCCCGCCCCA GCGAGCGGCA ACCTCGGCGC
    ACCAGCTCCG AGAGCCCGTC CCGCCTGCCC GTCCGCACGC CGGCCGCCGG GCCCGAGACG
    GTCAAGCGCT ACGCCTCCCT GCCTCACATC AGCGTGGCCC GCAGACCGGA TGCCACCATC
    CCCGAACCTG CGGCGGATGC CACCCGCCGC AGCAGCGCAG GGGAGGCCGG GCCGCTGCCC
    AGGGTGGCCG CGCCGGGCAC CACGTGGCGC CGCATCCGGG ACGAGGACGT CCCGCACATC
    CTGCGGAGCA CGCTGCCCGC CCGCGCCCTG CCGCTGCTGG GCTCCCCGCC GGAGGACGGC
    CCAGCCGGCC CACCACAGCG CAAGACCAGC GACGCCGTGG TCCAGACTGA AGACTTCGCG
    GCTGCGAAGA CCAACTCCAG CACGTCCCCG AGCATGGAGA GCAGGGCGCC CCCCGAGGCC
    ACGGCCGGCG GCCCCACCTC CCTTCTTGGC AGCGACGTGG ACGGGCCGCC CTCCAAGGCG
    CCCGCACCCA TCCCCTTCGT CCCCGCCAGC CGGCACGGCT CCCCCAGCCG CTCCGCCCGT
    GTCCCCCCTT TCAACTACGT GCCCAGCCCC ATGGTGGCGG CCACCGCTGA CTCCACCGTG
    GAGAAGGCCC CCACCCCGGT CCCCACCAGC CTCCTGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51193
    Clone ID Related Accession (Same CDS sequence) XM_002689175.5 , XM_005199027.3
    Accession Version XM_002689175.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689175.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTACGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CCACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CCCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689221.4
    CDS76..6909
    Translation

    Target ORF information:

    RefSeq Version XM_002689175.5
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689175.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTACGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CCACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CCCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51193
    Clone ID Related Accession (Same CDS sequence) XM_002689175.5 , XM_005199027.3
    Accession Version XM_005199027.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTACGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CCACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CCCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199084.2
    CDS76..6909
    Translation

    Target ORF information:

    RefSeq Version XM_005199027.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199027.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGCAGTTC
    TCAATGCAGA TGGATCTGAT CCGGCAGCAG CTGGAATTCG AGGCCCAACA CATCCGCTCG
    CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCGCA GATCCGTGCT
    TCCCGTCTGG AGCAGATCGA CAAGGAACTG CTGTCCGCGC AGGACCGAGT GCAGCAGACC
    GAGCCCCAGG CCTTGCTGGC AGTGAAGTCG ATGCCCATGG ACGAGGACGC TGAGAATGAG
    GTCCCCACGC ACCCTGAGGA TGGTGCCCCT CAGCCAGGCA ACAGTAAGGT GGAGGTGGTC
    TTCTGGCTCC TGTCCATGCT GGCAACGCGG GACCAGGAGG ACACGGCGCG GACGCTGCTC
    GCCATGTCCA GCTCACCCGA AAGCTGCGTG GCCATGCGCC GCTCAGGCTG TCTGCCGCTG
    CTGCTGCAGA TCCTGCACGG CACTGAGGCC GGGGCCGGGG GTCGCAACGG GACCCCCGGG
    GCTCCGGGAG CCAAGGACGC GCGCATGCGT GCCAACGCAG CGCTGCACAA TATCGTCTTC
    TCTCAGCCCG ACCAGGGGCT AGCACGCAAG GAGATGCGCG TGTTACACGT GCTGGAGCAG
    ATCCGCGCCT ACTGCGAGAC CTGTTGGGAC TGGCTGCAGG CCCGGGATGG CGGGCCTGAG
    GGCAGCGGCA CTGGTGCTGC GCCGGTGCCC ATCGAGCCAC AGATCTGTCA GGCCACCTGC
    GCCGTGATGA AGCTGTCCTT TGACGAGGAA TACCGCCGAG CTATGAATGA GCTGGGTGGG
    CTGCAGGCAG TGGCGGAGTT ACTGCAGGTT GACCATGAGA TGCACAAGAT GACCCGGGAC
    CCTCTCAACC TGGCCCTGCG CCGATACGCT GGCATGACCC TCACCAACCT CACCTTCGGG
    GACGTTGCCA ACAAGGCTGC ACTGTGCGCC CGCCGGGGCT GCATGGAGGC CATTGTGGCC
    CAGCTGGCAT CTGAGAGTGA GGAGCTGCAC CAGGTTGTGT CTAGCATCCT GCGCAACCTG
    TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AAGTGGGCAG CATGACTGCC
    CTGATGCAGT GCGTCCTGCG AGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTTAGCGCC
    CTGTGGAACC TCTCGGCGCA CAGCACGGAG AACAAGGCGG CCATCTGCCA GGTGGATGGT
    GCCCTGGGCT TCCTGGTAAG CACACTGACT TACAAGTGCC AGAGCAACTC GCTAGCCATC
    ATTGAGAGCG GTGGTGGCAT CCTGCGCAAT GTGTCCAGCC TCATCGCCAC CCGCGAGGAC
    TACAGGCAGG TGCTGCGAGA CCACAATTGC CTGCAGACGC TGCTGCAGCA CCTGACGTCG
    CACAGCCTGA CCATAGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC
    CCCCGCGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG GAACCTGGTG
    CACTCCAAGC ACAAAATGAT CGCCATGGGC AGCGCCGCCG CGCTGCGCAA CCTGCTGGCT
    CACCGGCCTG CCAAGTACCA GCCAGCGGCC ACCGCGGTCT CCCCGGGCTC CTGCGCGCCC
    AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACACACG GCACCTGGCT
    CAGGCACTTG ACCACCTGGA GAAGCAGGGC CTGCCGGAGG CCGAGACCGC CTGCAAGAAG
    CCCCTCCCGC CCCTGCGCCA CCTGGACGGC CTGGCCCAGG ACTACGCCTC TGACTCGGGC
    TGTTTTGACG ACGACGATGC GCCCTCCCTG GCTGCTGCCG CCACTGCCGC AGAGCCCGCC
    AGCCCCGCAG TGCTCTCCCT CTTCCTGGGC AGCCCCTTCC TGCAAGGCCA GGCGCTGGCC
    CGAGCCCCGC CCACCCGCCG AGGAGGCCTG GAGGTGGAGA AGGAGGCCGG TGGAGAGGCA
    GCCGTGGCAG CTAGGGCCAA GGCCAAGCTG GCGCTGGCGG TGGCACGCAT CGACAGGCTG
    GTAGAGGACA TCTCGGCCCT GCACACCTCA TCTGACGACA GCTTCAGCCT CAGCTCCGGG
    GACCCTGGCC AGGAGGCCCC ACGAGAAGGC CGTGCCCAAT CCTGCTCACC TTGCAGGGGG
    CCTGAGGGGG GGCGGCGGGA AGCAGGCAGC AGCCGGGCTC ACCCACTGCT GCGGCTCAAG
    GCAGCCCACG CCAGCCTTTC CAATGACAGT CTCAACAGCG GTAGCACCAG TGATGGGCAC
    TGTCCTCGCG AACACTCTCG GCCCTGCTCG CTGGCTGCTC TGGCCGAGCA CCGAGAGGGG
    CCCCCACGTG GTCAGGCGCG GCCCAGCCGG CTTGACCTCA ACCTGCCTGG AGGCCAGGCT
    GAGCCTGAGG CCCGGGATGC CAAGGCCACA GATGCCCACG TGCGTACCAT CAAGTTGTCG
    CCCACCTACC AGCACGTGCC GCTGCTGGAG GGCACCACTA GGGCGGGTGC GGGGTCCCTG
    GCCCCTGGGG CCCGGAAACA GGCCTGGCTG TCCACAGAAG GCCTGAGCAG TGTGCCTGAG
    AAGCTAGTGG TTGAGAAGGC ACCCCTCTGC CTGTCCCGCT GCAGCTCGCT GTCCTCACTG
    TCCTCAGCTG GCCGCCCGGG GCCCAGTGAG GCTGGGGACC TAGATGAGAG CGACTCATCC
    CTGGAGGGGC TGGAGGAGTC TGGCCCTGGT GAAACAGAGC TGGATGGGGC CTGGCGGGGC
    CCAGGGGCCG CCTCCCTGCC CATGGCCATC CCAGCGCCCC AGAGGGGTCG GGGCCTGGGG
    GTGGAGGATG CCACGCCGTC CAGCTCCTCT GAGAACTGCG TGCAGGAGAC ACCGCTGGTG
    CTGAGCCGCT GCAGCTCAGT CAGTTCACTG GGCAGCTTCG AGAGCCCATC CATTGCCAGC
    TCCATCCCCA GTGACCCATG CAGCGGGCTG GGCAGTGGCA CGGTCAGCCC CAGTGAGCTG
    CCTGATAGCC CCGGGCAAAC CATGCCTCCG AGCCGCAGCA AGACACCACC GCTGGCCCCA
    GCACCACCGG GCGAGCGGGA AGCCACCCAG TTCAGCCTGC AGTGGGAGAG TTACGTGAAG
    CGCTTCCTGG ACATCGCCGA CTGCCGGGAG CGCTGCCGGC TGCCCTCCGA GCTGGACGCA
    GGCAGCGTGC GTTTCACTGT GGAGAAGCCC GACGAGAACT TCTCGTGTGC CTCCAGCCTC
    AGCGCACTGG CCCTGCATGA GCTCTACGTG CAGAAAGACG TGGAGCTGCG GCTGCTGCCC
    CCTGCCTGCC CAGAGCGCGG CAGTGGGGGA GGCGGAGGCC CAGGGCACCG CCGACGGGAT
    GAGGCCAGTG GCTGTCTGGA AGGGCCGGCG TCCACCGACC AGGAGCTGGA GCTGCTGCGT
    GAGTGCCTGG GTGGGGCCGT GCCTGCCCGG CTCCGAAAGG TGGCCTCAGC ACTGGTGCCT
    GGCTGCCGCA CGCTGCCCGT GCCAGTCTAC ATGCTGGTGC CTGCCCCGGC CCGTGAGGAC
    GACTCCTGCA CTGACTCGGC TGAGGGCACT CCAGTCAACT TCTCCAGCAC CGCTTCCCTC
    AGCGACGAGA CCCTGCAGGG ACCCCCCAAG GATGGTGGGC GTTTGGATGG GAAGAAGCCC
    GTGGGTCGTG CTGCCTCCAC TAGGCAGTCT GCTGGGCAGC GGCACAGGGT CGGGGGCATG
    GGCCGGAGCA CAGAGCAGCC CCGGGGGGCT GGCAGTGGCA GGGCAGGGCT GGAGCTGCCC
    CTCCGGCGGC CCCCGAGCAC CTGCAGGGAC ACTGACAGCT CCCGCCCAGG CCAGGAGCAC
    GGGGATGGGG CTCTGCAGTC TCTCTGCCTC ACAACGCCCA CCGAGGAGGC TGTGTATTGT
    TTCTATGGCA ATGACTCGGA TGAGGAGCCG GCCACAGCAG TGGCAGCAGC ACCCCCACGG
    CGGGCATCTG CGATCCCCCG GGCGGTCAAG AGGGAGCGCC CCACTGGTGC CAGGAAGGAG
    GTGCAGGCTG TGCCCAAGGC TGCACCCAAG GCGGCACCGC CTGCCCGGGC CCAGCCCAGC
    CTCATTGCAG ATGAGACACC ACCGTGCTAC TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC
    GAACCTGAGC CCTCCGAGTG CATGGCCAGT CGGCCCCGGG TCCACAAGCC AGGGCTCACC
    AAGGACCTGG TCCCTGGGGG CAGGAAGGAT GGCTGCCCCA GCCCTCGGGC TGAGGCAGAG
    CTGCTCCGGC GTTGCATAGG CTCAGCCTTG CCCAGGCGCC AGCCCCAGGT GTCCGGCCCA
    AGGCGCCGCC CACCCCGAGA GGCCCAGCCA AAGGCGCGTG CAGCAGAAGG GCCCCGGGAG
    CGCGGTGAGG AGGCGGCCAG CTCAGACCAT GCCTCAGACC TGGACAGCAT CGAGTGGCGT
    GCCATCCAGG AGGGTGCCAA CTCTGTTGCC ACATGGCTGC ACCAGGCCGC AGCGGCGGCT
    GCACGTGAGG CCTCCTCAGA GTCTGATTCT GTTCTGTCCT TTGCAACAGG GCAGTCTGTG
    GGTTCCACCC TGCAGCCCAC TGTGCATAGG AAGGGGTGTC GGCCAAGGGC AGAGCGCCAA
    GCGGGTGGTG CCCTGCGGCC AGAGAAACCA GAAGGGGCCC TCACCCAGCG CAGCAGCGGG
    CCGGAGAAGC CGCGTGGCAC TCAGAAGGCC CCAAATGGGG TGCCGGCGGT GCTCCGGGGA
    CATACAGTGA TCTACATGCC CACCCCGGCC ACCCGGGCAC AGCCCAGAGG TGCCCCTGGT
    TCCCGCATTG TGGCAAGAAA GATGGGAGCG CCCAGCCCCG TGCAGCCGGC AGCCCCCACC
    AAAGCCCCCA ACCCAGGGCA GCAGCGGTCT AGGAGCCTGC ACCGGCCTGG CAAGATCTCA
    GAGCTGGCAA CACTCAGCCC CCCGCAGAGA AGTGCCACAC CACCAGCCCG CCTTGCCAAG
    ACCCCATCGT CAAGTTCCTC TCAGACCTCA CCGGCCTCCC AGCCTCTGCC CAGGAGGTCA
    CCCCCTGCTG CCCAGCCTGC AGGGCCCCTG CCCGGCCCCA GGGCCTCCCC AGTGCCCAAG
    ACTCCAGCAA GGGCCCTGCT GGCCAAGCAG CACAAGACGC AGAAGTCACC CGTGAGGATC
    CCCTTCATGC AGAGGCCTGC CAGGCGGGGG CCGCCGCCCT TGGCTAGGGC AGCCCCGGAG
    CCAGGCCCCA GGGGCCGGGT GGGCGCAGAA GGCGGCTCTG CAGCCCGAGG GGGCCGCCTG
    GGCCTGGTGC GCGTGGCCTC CGCCCGCTCC AGCGGCAGCG AATCCTCCGA CCGCTCGGGT
    TTCCGGCGGC AGCTGACCTT TATCAAGGAG TCGCCGGGCC TGATGCGGCG CCGACGCTCG
    GAACTGTCCG CACCCGAAGC CACCAACCCG ACCACCCAGG CTGCCTCGCC CTGCCGCGGC
    CGGCCAGCAC TACCCGCTGT CTTCCTCTGC TCCTCGCGCT GTGATGAGCT GCGGGCAGGC
    TCCCGGCAGG CCCCGGCCCC GCAGCGGGCC CACACGGCCC GGCCCCGCCC CAGCGAGCGG
    CAACCTCGGC GCACCAGCTC CGAGAGCCCG TCCCGCCTGC CCGTCCGCAC GCCGGCCGCC
    GGGCCCGAGA CGGTCAAGCG CTACGCCTCC CTGCCTCACA TCAGCGTGGC CCGCAGACCG
    GATGCCACCA TCCCCGAACC TGCGGCGGAT GCCACCCGCC GCAGCAGCGC AGGGGAGGCC
    GGGCCGCTGC CCAGGGTGGC CGCGCCGGGC ACCACGTGGC GCCGCATCCG GGACGAGGAC
    GTCCCGCACA TCCTGCGGAG CACGCTGCCC GCCCGCGCCC TGCCGCTGCT GGGCTCCCCG
    CCGGAGGACG GCCCAGCCGG CCCACCACAG CGCAAGACCA GCGACGCCGT GGTCCAGACT
    GAAGACTTCG CGGCTGCGAA GACCAACTCC AGCACGTCCC CGAGCATGGA GAGCAGGGCG
    CCCCCCGAGG CCACGGCCGG CGGCCCCACC TCCCTTCTTG GCAGCGACGT GGACGGGCCG
    CCCTCCAAGG CGCCCGCACC CATCCCCTTC GTCCCCGCCA GCCGGCACGG CTCCCCCAGC
    CGCTCCGCCC GTGTCCCCCC TTTCAACTAC GTGCCCAGCC CCATGGTGGC GGCCACCGCT
    GACTCCACCG TGGAGAAGGC CCCCACCCCG GTCCCCACCA GCCTCCTGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51194
    Clone ID Related Accession (Same CDS sequence) XM_005199028.3 , XM_005209327.3
    Accession Version XM_005199028.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTACGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCCA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCCC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199085.2
    CDS76..6906
    Translation

    Target ORF information:

    RefSeq Version XM_005199028.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199028.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTACGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCCA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCCC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51194
    Clone ID Related Accession (Same CDS sequence) XM_005199028.3 , XM_005209327.3
    Accession Version XM_005209327.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTACGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCCA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCCC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209384.2
    CDS76..6906
    Translation

    Target ORF information:

    RefSeq Version XM_005209327.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatosis polyposis coli 2 (APC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209327.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGGGCTCC TGGGGCTGCT GAGCCTGCTG CACTCGGTCT TCTTTGGGGA CCAGGCACTA 
    CAGGCGCTGA AGATGACGAG CTCAGTGGCA GCCTATGAGC AGCTGGTGCG GCAGGTGGAG
    GCCTTGAAGG CCGAGAACAG TCACCTGCGG CAGGAGCTTC GGGATAATTC AAGCCACCTG
    TCCAAGCTGG AGACAGAGAC GTCGGGAATG AAGGCGGTCC TGCAGCACTT ACAGGGCAAG
    CTAGAGCAGG AGGCCCGTGT GTTGGTGTCC TCCGGGCAGA CTGAGGTGCT GGAACAGCTG
    AAAGCCCTGC AGATGGACAT CACCAGCCTG TATAACCTCA AGTTCCAGCC CCCAGCTCTG
    GCCCCTGAAC CCACTACCAG GACCCCTGAG GGAAGCCCGG TGCACAGCTC TGGGCCCTCC
    AAGGAGAATT TTGGGGAGCT GAGCCGGGCC ACCATTCGGC TCCTAGAGGA ACTGGACCGG
    GAACGGTGTT TCCTGTTGAA TGAGATTGAG AAGGAGGAAA AGGAGAAGCT CTGGTATTAC
    TCGCAGTTGC AGGGGCTATC TAAGCGCTTG GATGAACTCC CGCACGTGGA GACGTTCTCA
    ATGCAGATGG ATCTGATCCG GCAGCAGCTG GAATTCGAGG CCCAACACAT CCGCTCGCTG
    ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCGCAGAT CCGTGCTTCC
    CGTCTGGAGC AGATCGACAA GGAACTGCTG TCCGCGCAGG ACCGAGTGCA GCAGACCGAG
    CCCCAGGCCT TGCTGGCAGT GAAGTCGATG CCCATGGACG AGGACGCTGA GAATGAGGTC
    CCCACGCACC CTGAGGATGG TGCCCCTCAG CCAGGCAACA GTAAGGTGGA GGTGGTCTTC
    TGGCTCCTGT CCATGCTGGC AACGCGGGAC CAGGAGGACA CGGCGCGGAC GCTGCTCGCC
    ATGTCCAGCT CACCCGAAAG CTGCGTGGCC ATGCGCCGCT CAGGCTGTCT GCCGCTGCTG
    CTGCAGATCC TGCACGGCAC TGAGGCCGGG GCCGGGGGTC GCAACGGGAC CCCCGGGGCT
    CCGGGAGCCA AGGACGCGCG CATGCGTGCC AACGCAGCGC TGCACAATAT CGTCTTCTCT
    CAGCCCGACC AGGGGCTAGC ACGCAAGGAG ATGCGCGTGT TACACGTGCT GGAGCAGATC
    CGCGCCTACT GCGAGACCTG TTGGGACTGG CTGCAGGCCC GGGATGGCGG GCCTGAGGGC
    AGCGGCACTG GTGCTGCGCC GGTGCCCATC GAGCCACAGA TCTGTCAGGC CACCTGCGCC
    GTGATGAAGC TGTCCTTTGA CGAGGAATAC CGCCGAGCTA TGAATGAGCT GGGTGGGCTG
    CAGGCAGTGG CGGAGTTACT GCAGGTTGAC CATGAGATGC ACAAGATGAC CCGGGACCCT
    CTCAACCTGG CCCTGCGCCG ATACGCTGGC ATGACCCTCA CCAACCTCAC CTTCGGGGAC
    GTTGCCAACA AGGCTGCACT GTGCGCCCGC CGGGGCTGCA TGGAGGCCAT TGTGGCCCAG
    CTGGCATCTG AGAGTGAGGA GCTGCACCAG GTTGTGTCTA GCATCCTGCG CAACCTGTCC
    TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAAG TGGGCAGCAT GACTGCCCTG
    ATGCAGTGCG TCCTGCGAGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT TAGCGCCCTG
    TGGAACCTCT CGGCGCACAG CACGGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGTGCC
    CTGGGCTTCC TGGTAAGCAC ACTGACTTAC AAGTGCCAGA GCAACTCGCT AGCCATCATT
    GAGAGCGGTG GTGGCATCCT GCGCAATGTG TCCAGCCTCA TCGCCACCCG CGAGGACTAC
    AGGCAGGTGC TGCGAGACCA CAATTGCCTG CAGACGCTGC TGCAGCACCT GACGTCGCAC
    AGCCTGACCA TAGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCCCC
    CGCGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGGAA CCTGGTGCAC
    TCCAAGCACA AAATGATCGC CATGGGCAGC GCCGCCGCGC TGCGCAACCT GCTGGCTCAC
    CGGCCTGCCA AGTACCAGCC AGCGGCCACC GCGGTCTCCC CGGGCTCCTG CGCGCCCAGC
    CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACACACGGCA CCTGGCTCAG
    GCACTTGACC ACCTGGAGAA GCAGGGCCTG CCGGAGGCCG AGACCGCCTG CAAGAAGCCC
    CTCCCGCCCC TGCGCCACCT GGACGGCCTG GCCCAGGACT ACGCCTCTGA CTCGGGCTGT
    TTTGACGACG ACGATGCGCC CTCCCTGGCT GCTGCCGCCA CTGCCGCAGA GCCCGCCAGC
    CCCGCAGTGC TCTCCCTCTT CCTGGGCAGC CCCTTCCTGC AAGGCCAGGC GCTGGCCCGA
    GCCCCGCCCA CCCGCCGAGG AGGCCTGGAG GTGGAGAAGG AGGCCGGTGG AGAGGCAGCC
    GTGGCAGCTA GGGCCAAGGC CAAGCTGGCG CTGGCGGTGG CACGCATCGA CAGGCTGGTA
    GAGGACATCT CGGCCCTGCA CACCTCATCT GACGACAGCT TCAGCCTCAG CTCCGGGGAC
    CCTGGCCAGG AGGCCCCACG AGAAGGCCGT GCCCAATCCT GCTCACCTTG CAGGGGGCCT
    GAGGGGGGGC GGCGGGAAGC AGGCAGCAGC CGGGCTCACC CACTGCTGCG GCTCAAGGCA
    GCCCACGCCA GCCTTTCCAA TGACAGTCTC AACAGCGGTA GCACCAGTGA TGGGCACTGT
    CCTCGCGAAC ACTCTCGGCC CTGCTCGCTG GCTGCTCTGG CCGAGCACCG AGAGGGGCCC
    CCACGTGGTC AGGCGCGGCC CAGCCGGCTT GACCTCAACC TGCCTGGAGG CCAGGCTGAG
    CCTGAGGCCC GGGATGCCAA GGCCACAGAT GCCCACGTGC GTACCATCAA GTTGTCGCCC
    ACCTACCAGC ACGTGCCGCT GCTGGAGGGC ACCACTAGGG CGGGTGCGGG GTCCCTGGCC
    CCTGGGGCCC GGAAACAGGC CTGGCTGTCC ACAGAAGGCC TGAGCAGTGT GCCTGAGAAG
    CTAGTGGTTG AGAAGGCACC CCTCTGCCTG TCCCGCTGCA GCTCGCTGTC CTCACTGTCC
    TCAGCTGGCC GCCCGGGGCC CAGTGAGGCT GGGGACCTAG ATGAGAGCGA CTCATCCCTG
    GAGGGGCTGG AGGAGTCTGG CCCTGGTGAA ACAGAGCTGG ATGGGGCCTG GCGGGGCCCA
    GGGGCCGCCT CCCTGCCCAT GGCCATCCCA GCGCCCCAGA GGGGTCGGGG CCTGGGGGTG
    GAGGATGCCA CGCCGTCCAG CTCCTCTGAG AACTGCGTGC AGGAGACACC GCTGGTGCTG
    AGCCGCTGCA GCTCAGTCAG TTCACTGGGC AGCTTCGAGA GCCCATCCAT TGCCAGCTCC
    ATCCCCAGTG ACCCATGCAG CGGGCTGGGC AGTGGCACGG TCAGCCCCAG TGAGCTGCCT
    GATAGCCCCG GGCAAACCAT GCCTCCGAGC CGCAGCAAGA CACCACCGCT GGCCCCAGCA
    CCACCGGGCG AGCGGGAAGC CACCCAGTTC AGCCTGCAGT GGGAGAGTTA CGTGAAGCGC
    TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CCTCCGAGCT GGACGCAGGC
    AGCGTGCGTT TCACTGTGGA GAAGCCCGAC GAGAACTTCT CGTGTGCCTC CAGCCTCAGC
    GCACTGGCCC TGCATGAGCT CTACGTGCAG AAAGACGTGG AGCTGCGGCT GCTGCCCCCT
    GCCTGCCCAG AGCGCGGCAG TGGGGGAGGC GGAGGCCCAG GGCACCGCCG ACGGGATGAG
    GCCAGTGGCT GTCTGGAAGG GCCGGCGTCC ACCGACCAGG AGCTGGAGCT GCTGCGTGAG
    TGCCTGGGTG GGGCCGTGCC TGCCCGGCTC CGAAAGGTGG CCTCAGCACT GGTGCCTGGC
    TGCCGCACGC TGCCCGTGCC AGTCTACATG CTGGTGCCTG CCCCGGCCCG TGAGGACGAC
    TCCTGCACTG ACTCGGCTGA GGGCACTCCA GTCAACTTCT CCAGCACCGC TTCCCTCAGC
    GACGAGACCC TGCAGGGACC CCCCAAGGAT GGTGGGCGTT TGGATGGGAA GAAGCCCGTG
    GGTCGTGCTG CCTCCACTAG GCAGTCTGCT GGGCAGCGGC ACAGGGTCGG GGGCATGGGC
    CGGAGCACAG AGCAGCCCCG GGGGGCTGGC AGTGGCAGGG CAGGGCTGGA GCTGCCCCTC
    CGGCGGCCCC CGAGCACCTG CAGGGACACT GACAGCTCCC GCCCAGGCCA GGAGCACGGG
    GATGGGGCTC TGCAGTCTCT CTGCCTCACA ACGCCCACCG AGGAGGCTGT GTATTGTTTC
    TATGGCAATG ACTCGGATGA GGAGCCGGCC ACAGCAGTGG CAGCAGCACC CCCACGGCGG
    GCATCTGCGA TCCCCCGGGC GGTCAAGAGG GAGCGCCCCA CTGGTGCCAG GAAGGAGGTG
    CAGGCTGTGC CCAAGGCTGC ACCCAAGGCG GCACCGCCTG CCCGGGCCCA GCCCAGCCTC
    ATTGCAGATG AGACACCACC GTGCTACTCC CTGAGCTCCT CCGCCAGCTC CCTCAGCGAA
    CCTGAGCCCT CCGAGTGCAT GGCCAGTCGG CCCCGGGTCC ACAAGCCAGG GCTCACCAAG
    GACCTGGTCC CTGGGGGCAG GAAGGATGGC TGCCCCAGCC CTCGGGCTGA GGCAGAGCTG
    CTCCGGCGTT GCATAGGCTC AGCCTTGCCC AGGCGCCAGC CCCAGGTGTC CGGCCCAAGG
    CGCCGCCCAC CCCGAGAGGC CCAGCCAAAG GCGCGTGCAG CAGAAGGGCC CCGGGAGCGC
    GGTGAGGAGG CGGCCAGCTC AGACCATGCC TCAGACCTGG ACAGCATCGA GTGGCGTGCC
    ATCCAGGAGG GTGCCAACTC TGTTGCCACA TGGCTGCACC AGGCCGCAGC GGCGGCTGCA
    CGTGAGGCCT CCTCAGAGTC TGATTCTGTT CTGTCCTTTG CAACAGGGCA GTCTGTGGGT
    TCCACCCTGC AGCCCACTGT GCATAGGAAG GGGTGTCGGC CAAGGGCAGA GCGCCAAGCG
    GGTGGTGCCC TGCGGCCAGA GAAACCAGAA GGGGCCCTCA CCCAGCGCAG CAGCGGGCCG
    GAGAAGCCGC GTGGCACTCA GAAGGCCCCA AATGGGGTGC CGGCGGTGCT CCGGGGACAT
    ACAGTGATCT ACATGCCCAC CCCGGCCACC CGGGCACAGC CCAGAGGTGC CCCTGGTTCC
    CGCATTGTGG CAAGAAAGAT GGGAGCGCCC AGCCCCGTGC AGCCGGCAGC CCCCACCAAA
    GCCCCCAACC CAGGGCAGCA GCGGTCTAGG AGCCTGCACC GGCCTGGCAA GATCTCAGAG
    CTGGCAACAC TCAGCCCCCC GCAGAGAAGT GCCACACCAC CAGCCCGCCT TGCCAAGACC
    CCATCGTCAA GTTCCTCTCA GACCTCACCG GCCTCCCAGC CTCTGCCCAG GAGGTCACCC
    CCTGCTGCCC AGCCTGCAGG GCCCCTGCCC GGCCCCAGGG CCTCCCCAGT GCCCAAGACT
    CCAGCAAGGG CCCTGCTGGC CAAGCAGCAC AAGACGCAGA AGTCACCCGT GAGGATCCCC
    TTCATGCAGA GGCCTGCCAG GCGGGGGCCG CCGCCCTTGG CTAGGGCAGC CCCGGAGCCA
    GGCCCCAGGG GCCGGGTGGG CGCAGAAGGC GGCTCTGCAG CCCGAGGGGG CCGCCTGGGC
    CTGGTGCGCG TGGCCTCCGC CCGCTCCAGC GGCAGCGAAT CCTCCGACCG CTCGGGTTTC
    CGGCGGCAGC TGACCTTTAT CAAGGAGTCG CCGGGCCTGA TGCGGCGCCG ACGCTCGGAA
    CTGTCCGCAC CCGAAGCCAC CAACCCGACC ACCCAGGCTG CCTCGCCCTG CCGCGGCCGG
    CCAGCACTAC CCGCTGTCTT CCTCTGCTCC TCGCGCTGTG ATGAGCTGCG GGCAGGCTCC
    CGGCAGGCCC CGGCCCCGCA GCGGGCCCAC ACGGCCCGGC CCCGCCCCAG CGAGCGGCAA
    CCTCGGCGCA CCAGCTCCGA GAGCCCGTCC CGCCTGCCCG TCCGCACGCC GGCCGCCGGG
    CCCGAGACGG TCAAGCGCTA CGCCTCCCTG CCTCACATCA GCGTGGCCCG CAGACCGGAT
    GCCACCATCC CCGAACCTGC GGCGGATGCC ACCCGCCGCA GCAGCGCAGG GGAGGCCGGG
    CCGCTGCCCA GGGTGGCCGC GCCGGGCACC ACGTGGCGCC GCATCCGGGA CGAGGACGTC
    CCGCACATCC TGCGGAGCAC GCTGCCCGCC CGCGCCCTGC CGCTGCTGGG CTCCCCGCCG
    GAGGACGGCC CAGCCGGCCC ACCACAGCGC AAGACCAGCG ACGCCGTGGT CCAGACTGAA
    GACTTCGCGG CTGCGAAGAC CAACTCCAGC ACGTCCCCGA GCATGGAGAG CAGGGCGCCC
    CCCGAGGCCA CGGCCGGCGG CCCCACCTCC CTTCTTGGCA GCGACGTGGA CGGGCCGCCC
    TCCAAGGCGC CCGCACCCAT CCCCTTCGTC CCCGCCAGCC GGCACGGCTC CCCCAGCCGC
    TCCGCCCGTG TCCCCCCTTT CAACTACGTG CCCAGCCCCA TGGTGGCGGC CACCGCTGAC
    TCCACCGTGG AGAAGGCCCC CACCCCGGTC CCCACCAGCC TCCTGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.