GLS cDNA ORF clone, Bos taurus(cattle)

The following GLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50979 XM_010802152.2
Latest version!
Bos taurus glutaminase (GLS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa227338 XM_024975045.1
Latest version!
Bos taurus glutaminase (GLS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa39961 NM_001077964.2
Latest version!
Bos taurus glutaminase (GLS), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OBa16525 XM_005202579.3
Latest version!
Bos taurus glutaminase (GLS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa16526 XM_005202580.4
Latest version!
Bos taurus glutaminase (GLS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
View Old Accession Versions >>
OBa16525 XM_005202579.2 Bos taurus glutaminase (GLS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa50979 XM_010802152.1 Bos taurus glutaminase (GLS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa16526 XM_005202580.3 Bos taurus glutaminase (GLS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50979
    Clone ID Related Accession (Same CDS sequence) XM_010802152.1 , XM_010802152.2
    Accession Version XM_010802152.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103844.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAATATT TTGACAACTG CAACAGTTCA ATGAAGTTGC AAGGAGTGAA TAATGCTGAA 
    AAATTTGACT ATGTAATGCA GTTTTTAAAT AAGATGGCTG GTAATGAATA TGTTGGATTC
    AGTAATGCAA CGTTTCAGTC TGAAAGAGAA AGTGGAGATC GAAATTTTGC AATAGGATAT
    TACTTAAAGG AAAAAAAGTG TTTCCCAGAA GGCACAGACA TGGTTGGTAT ATTGGACTTC
    TATTTCCAGC TGTGCTCCAT CGAAGTAACT TGTGAGTCAG CTAGTGTGAT GGCAGCAACA
    CTGGCTAATG GTGGCTTCTG CCCAATCACT GGTGAAAGAG TACTGAGTCC TGAAGCAGTT
    CGAAATACAC TCAGTTTGAT GCATTCCTGT GGCATGTATG ATTTCTCAGG GCAGTTTGCT
    TTCCATGTTG GTCTTCCTGC AAAATCTGGA GTTGCTGGAG GCATTCTTTT AGTTGTCCCC
    AATGTCATGG GCATGATGTG CTGGTCTCCT CCTCTGGACA AGATGGGCAA CAGTGTTAAG
    GGAATTCACT TCTGTCATGA TCTTGTTTCT CTGTGTAATT TCCATAACTA TGATAATTTG
    AGACACTTTG CTAAAAAACT TGATCCTCGA AGAGAAGGTG GTGATCAGAG GGTAAAGTCA
    GTGATAAACC TTTTGTTTGC TGCATATACT GGAGATGTGT CTGCACTTCG AAGGTTTGCT
    TTGTCAGCCA TGGACATGGA ACAGCGAGAC TACGATTCTA GAACGGCACT CCACGTAGCT
    GCTGCAGAGG GTCATGTTGA AGTTGTTAAA TTTCTGCTGG AAGCCTGCAA AGTAAATCCT
    TTCCCCAAGG ACAGGTGGAA TAACACTCCC ATGGACGAAG CGCTGCACTT TGGGCACCAC
    GACGTATTTA AAATCCTGCA GGAATACCAA GTCCAGTACA CGCCTCAAGG AGATTCCGAC
    AACGGAAAGG AGAATCAAAC GGTCCATAAG AATCTCGATG GACTGTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800454.1
    CDS3085..4095
    Translation

    Target ORF information:

    RefSeq Version XM_010802152.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAATATT TTGACAACTG CAACAGTTCA ATGAAGTTGC AAGGAGTGAA TAATGCTGAA 
    AAATTTGACT ATGTAATGCA GTTTTTAAAT AAGATGGCTG GTAATGAATA TGTTGGATTC
    AGTAATGCAA CGTTTCAGTC TGAAAGAGAA AGTGGAGATC GAAATTTTGC AATAGGATAT
    TACTTAAAGG AAAAAAAGTG TTTCCCAGAA GGCACAGACA TGGTTGGTAT ATTGGACTTC
    TATTTCCAGC TGTGCTCCAT CGAAGTAACT TGTGAGTCAG CTAGTGTGAT GGCAGCAACA
    CTGGCTAATG GTGGCTTCTG CCCAATCACT GGTGAAAGAG TACTGAGTCC TGAAGCAGTT
    CGAAATACAC TCAGTTTGAT GCATTCCTGT GGCATGTATG ATTTCTCAGG GCAGTTTGCT
    TTCCATGTTG GTCTTCCTGC AAAATCTGGA GTTGCTGGAG GCATTCTTTT AGTTGTCCCC
    AATGTCATGG GCATGATGTG CTGGTCTCCT CCTCTGGACA AGATGGGCAA CAGTGTTAAG
    GGAATTCACT TCTGTCATGA TCTTGTTTCT CTGTGTAATT TCCATAACTA TGATAATTTG
    AGACACTTTG CTAAAAAACT TGATCCTCGA AGAGAAGGTG GTGATCAGAG GGTAAAGTCA
    GTGATAAACC TTTTGTTTGC TGCATATACT GGAGATGTGT CTGCACTTCG AAGGTTTGCT
    TTGTCAGCCA TGGACATGGA ACAGCGAGAC TACGATTCTA GAACGGCACT CCACGTAGCT
    GCTGCAGAGG GTCATGTTGA AGTTGTTAAA TTTCTGCTGG AAGCCTGCAA AGTAAATCCT
    TTCCCCAAGG ACAGGTGGAA TAACACTCCC ATGGACGAAG CGCTGCACTT TGGGCACCAC
    GACGTATTTA AAATCCTGCA GGAATACCAA GTCCAGTACA CGCCTCAAGG AGATTCCGAC
    AACGGAAAGG AGAATCAAAC GGTCCATAAG AATCTCGATG GACTGTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50979
    Clone ID Related Accession (Same CDS sequence) XM_010802152.1 , XM_010802152.2
    Accession Version XM_010802152.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAATATT TTGACAACTG CAACAGTTCA ATGAAGTTGC AAGGAGTGAA TAATGCTGAA 
    AAATTTGACT ATGTAATGCA GTTTTTAAAT AAGATGGCTG GTAATGAATA TGTTGGATTC
    AGTAATGCAA CGTTTCAGTC TGAAAGAGAA AGTGGAGATC GAAATTTTGC AATAGGATAT
    TACTTAAAGG AAAAAAAGTG TTTCCCAGAA GGCACAGACA TGGTTGGTAT ATTGGACTTC
    TATTTCCAGC TGTGCTCCAT CGAAGTAACT TGTGAGTCAG CTAGTGTGAT GGCAGCAACA
    CTGGCTAATG GTGGCTTCTG CCCAATCACT GGTGAAAGAG TACTGAGTCC TGAAGCAGTT
    CGAAATACAC TCAGTTTGAT GCATTCCTGT GGCATGTATG ATTTCTCAGG GCAGTTTGCT
    TTCCATGTTG GTCTTCCTGC AAAATCTGGA GTTGCTGGAG GCATTCTTTT AGTTGTCCCC
    AATGTCATGG GCATGATGTG CTGGTCTCCT CCTCTGGACA AGATGGGCAA CAGTGTTAAG
    GGAATTCACT TCTGTCATGA TCTTGTTTCT CTGTGTAATT TCCATAACTA TGATAATTTG
    AGACACTTTG CTAAAAAACT TGATCCTCGA AGAGAAGGTG GTGATCAGAG GGTAAAGTCA
    GTGATAAACC TTTTGTTTGC TGCATATACT GGAGATGTGT CTGCACTTCG AAGGTTTGCT
    TTGTCAGCCA TGGACATGGA ACAGCGAGAC TACGATTCTA GAACGGCACT CCACGTAGCT
    GCTGCAGAGG GTCATGTTGA AGTTGTTAAA TTTCTGCTGG AAGCCTGCAA AGTAAATCCT
    TTCCCCAAGG ACAGGTGGAA TAACACTCCC ATGGACGAAG CGCTGCACTT TGGGCACCAC
    GACGTATTTA AAATCCTGCA GGAATACCAA GTCCAGTACA CGCCTCAAGG AGATTCCGAC
    AACGGAAAGG AGAATCAAAC GGTCCATAAG AATCTCGATG GACTGTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800454.1
    CDS12..1022
    Translation

    Target ORF information:

    RefSeq Version XM_010802152.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAATATT TTGACAACTG CAACAGTTCA ATGAAGTTGC AAGGAGTGAA TAATGCTGAA 
    AAATTTGACT ATGTAATGCA GTTTTTAAAT AAGATGGCTG GTAATGAATA TGTTGGATTC
    AGTAATGCAA CGTTTCAGTC TGAAAGAGAA AGTGGAGATC GAAATTTTGC AATAGGATAT
    TACTTAAAGG AAAAAAAGTG TTTCCCAGAA GGCACAGACA TGGTTGGTAT ATTGGACTTC
    TATTTCCAGC TGTGCTCCAT CGAAGTAACT TGTGAGTCAG CTAGTGTGAT GGCAGCAACA
    CTGGCTAATG GTGGCTTCTG CCCAATCACT GGTGAAAGAG TACTGAGTCC TGAAGCAGTT
    CGAAATACAC TCAGTTTGAT GCATTCCTGT GGCATGTATG ATTTCTCAGG GCAGTTTGCT
    TTCCATGTTG GTCTTCCTGC AAAATCTGGA GTTGCTGGAG GCATTCTTTT AGTTGTCCCC
    AATGTCATGG GCATGATGTG CTGGTCTCCT CCTCTGGACA AGATGGGCAA CAGTGTTAAG
    GGAATTCACT TCTGTCATGA TCTTGTTTCT CTGTGTAATT TCCATAACTA TGATAATTTG
    AGACACTTTG CTAAAAAACT TGATCCTCGA AGAGAAGGTG GTGATCAGAG GGTAAAGTCA
    GTGATAAACC TTTTGTTTGC TGCATATACT GGAGATGTGT CTGCACTTCG AAGGTTTGCT
    TTGTCAGCCA TGGACATGGA ACAGCGAGAC TACGATTCTA GAACGGCACT CCACGTAGCT
    GCTGCAGAGG GTCATGTTGA AGTTGTTAAA TTTCTGCTGG AAGCCTGCAA AGTAAATCCT
    TTCCCCAAGG ACAGGTGGAA TAACACTCCC ATGGACGAAG CGCTGCACTT TGGGCACCAC
    GACGTATTTA AAATCCTGCA GGAATACCAA GTCCAGTACA CGCCTCAAGG AGATTCCGAC
    AACGGAAAGG AGAATCAAAC GGTCCATAAG AATCTCGATG GACTGTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227338
    Clone ID Related Accession (Same CDS sequence) XM_024975045.1
    Accession Version XM_024975045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGCGAGT TTTTGAAAAT ACATATGAAA ATAGCCAGGA TTGAATGTGA CGGTAAAATA 
    AAACAGGGTC TGTTACCTAG TTTGGAAGAT TTGCTGTTCT ATACAATTGC AGAAGGACAA
    GAGAAAATAC CTGTTCATAA ATTTATTACA GCACTCAAAT CTACAGGATT GCGAACGTCT
    GATCCCAGGT TGAAAGAGTG TATGGATATG TTAAGATTAA CTCTTCAAAC AACATCAGAT
    GGTGTCATGC TAGACAAAGA TCTTTTTAAA AAATGTGTTC AAAGCAACAT TGTTTTGTTG
    ACACAAGCCT TTAGAAGAAA GTTTGTTATT CCTGACTTTA TGTCTTTTAC TTCACATATC
    GATGAATTAT ATGAGAGTGC TAAAAAGCAG TCTGGAGGAA AGGTTGCAGA TTATATTCCT
    CAACTAGCCA AGTTCAGCCC TGATTTGTGG GGTGTATCTG TTTGTACAGT AGATGGACAG
    AGGCATTCTG TTGGAGATAC CAAAGTTCCA TTTTGTCTTC AGTCCTGTGT AAAGCCTTTG
    AAATATGCCA TTGCTGTTAA TGACCTTGGA ACAGAATATG TGCATCGATA TGTTGGGAAA
    GAGCCAAGTG GATTAAGGTT CAACAAACTG TTTTTAAATG AAGATGATAA ACCACACAAT
    CCTATGGTAA ATGCTGGAGC AATTGTTGTG ACTTCATTAA TAAAGCAAGG AGTGAATAAT
    GCTGAAAAAT TTGACTATGT AATGCAGTTT TTAAATAAGA TGGCTGGTAA TGAATATGTT
    GGATTCAGTA ATGCAACGTT TCAGTCTGAA AGAGAAAGTG GAGATCGAAA TTTTGCAATA
    GGATATTACT TAAAGGAAAA AAAGTGTTTC CCAGAAGGCA CAGACATGGT TGGTATATTG
    GACTTCTATT TCCAGCTGTG CTCCATCGAA GTAACTTGTG AGTCAGCTAG TGTGATGGCA
    GCAACACTGG CTAATGGTGG CTTCTGCCCA ATCACTGGTG AAAGAGTACT GAGTCCTGAA
    GCAGTTCGAA ATACACTCAG TTTGATGCAT TCCTGTGGCA TGTATGATTT CTCAGGGCAG
    TTTGCTTTCC ATGTTGGTCT TCCTGCAAAA TCTGGAGTTG CTGGAGGCAT TCTTTTAGTT
    GTCCCCAATG TCATGGGCAT GATGTGCTGG TCTCCTCCTC TGGACAAGAT GGGCAACAGT
    GTTAAGGGAA TTCACTTCTG TCATGATCTT GTTTCTCTGT GTAATTTCCA TAACTATGAT
    AATTTGAGAC ACTTTGCTAA AAAACTTGAT CCTCGAAGAG AAGGTGGTGA TCAGAGGGTA
    AAGTCAGTGA TAAACCTTTT GTTTGCTGCA TATACTGGAG ATGTGTCTGC ACTTCGAAGG
    TTTGCTTTGT CAGCCATGGA CATGGAACAG CGAGACTACG ATTCTAGAAC GGCACTCCAC
    GTAGCTGCTG CAGAGGGTCA TGTTGAAGTT GTTAAATTTC TGCTGGAAGC CTGCAAAGTA
    AATCCTTTCC CCAAGGACAG GTGGAATAAC ACTCCCATGG ACGAAGCGCT GCACTTTGGG
    CACCACGACG TATTTAAAAT CCTGCAGGAA TACCAAGTCC AGTACACGCC TCAAGGAGAT
    TCCGACAACG GAAAGGAGAA TCAAACGGTC CATAAGAATC TCGATGGACT GTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830813.1
    CDS371..2047
    Translation

    Target ORF information:

    RefSeq Version XM_024975045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGCGAGT TTTTGAAAAT ACATATGAAA ATAGCCAGGA TTGAATGTGA CGGTAAAATA 
    AAACAGGGTC TGTTACCTAG TTTGGAAGAT TTGCTGTTCT ATACAATTGC AGAAGGACAA
    GAGAAAATAC CTGTTCATAA ATTTATTACA GCACTCAAAT CTACAGGATT GCGAACGTCT
    GATCCCAGGT TGAAAGAGTG TATGGATATG TTAAGATTAA CTCTTCAAAC AACATCAGAT
    GGTGTCATGC TAGACAAAGA TCTTTTTAAA AAATGTGTTC AAAGCAACAT TGTTTTGTTG
    ACACAAGCCT TTAGAAGAAA GTTTGTTATT CCTGACTTTA TGTCTTTTAC TTCACATATC
    GATGAATTAT ATGAGAGTGC TAAAAAGCAG TCTGGAGGAA AGGTTGCAGA TTATATTCCT
    CAACTAGCCA AGTTCAGCCC TGATTTGTGG GGTGTATCTG TTTGTACAGT AGATGGACAG
    AGGCATTCTG TTGGAGATAC CAAAGTTCCA TTTTGTCTTC AGTCCTGTGT AAAGCCTTTG
    AAATATGCCA TTGCTGTTAA TGACCTTGGA ACAGAATATG TGCATCGATA TGTTGGGAAA
    GAGCCAAGTG GATTAAGGTT CAACAAACTG TTTTTAAATG AAGATGATAA ACCACACAAT
    CCTATGGTAA ATGCTGGAGC AATTGTTGTG ACTTCATTAA TAAAGCAAGG AGTGAATAAT
    GCTGAAAAAT TTGACTATGT AATGCAGTTT TTAAATAAGA TGGCTGGTAA TGAATATGTT
    GGATTCAGTA ATGCAACGTT TCAGTCTGAA AGAGAAAGTG GAGATCGAAA TTTTGCAATA
    GGATATTACT TAAAGGAAAA AAAGTGTTTC CCAGAAGGCA CAGACATGGT TGGTATATTG
    GACTTCTATT TCCAGCTGTG CTCCATCGAA GTAACTTGTG AGTCAGCTAG TGTGATGGCA
    GCAACACTGG CTAATGGTGG CTTCTGCCCA ATCACTGGTG AAAGAGTACT GAGTCCTGAA
    GCAGTTCGAA ATACACTCAG TTTGATGCAT TCCTGTGGCA TGTATGATTT CTCAGGGCAG
    TTTGCTTTCC ATGTTGGTCT TCCTGCAAAA TCTGGAGTTG CTGGAGGCAT TCTTTTAGTT
    GTCCCCAATG TCATGGGCAT GATGTGCTGG TCTCCTCCTC TGGACAAGAT GGGCAACAGT
    GTTAAGGGAA TTCACTTCTG TCATGATCTT GTTTCTCTGT GTAATTTCCA TAACTATGAT
    AATTTGAGAC ACTTTGCTAA AAAACTTGAT CCTCGAAGAG AAGGTGGTGA TCAGAGGGTA
    AAGTCAGTGA TAAACCTTTT GTTTGCTGCA TATACTGGAG ATGTGTCTGC ACTTCGAAGG
    TTTGCTTTGT CAGCCATGGA CATGGAACAG CGAGACTACG ATTCTAGAAC GGCACTCCAC
    GTAGCTGCTG CAGAGGGTCA TGTTGAAGTT GTTAAATTTC TGCTGGAAGC CTGCAAAGTA
    AATCCTTTCC CCAAGGACAG GTGGAATAAC ACTCCCATGG ACGAAGCGCT GCACTTTGGG
    CACCACGACG TATTTAAAAT CCTGCAGGAA TACCAAGTCC AGTACACGCC TCAAGGAGAT
    TCCGACAACG GAAAGGAGAA TCAAACGGTC CATAAGAATC TCGATGGACT GTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39961
    Clone ID Related Accession (Same CDS sequence) NM_001077964.2
    Accession Version NM_001077964.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126537.1. On Aug 30, 2012 this sequence version replaced NM_001077964.1. ##Evidence-Data-START## Transcript exon combination :: BC126537.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG CTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCATTC CTTTGGACCA
    TTGGACTATG AAAGTCTCCA ACAAGAACTT GCTTTAAAAG AGACAGTATG GAAAAAAGTG
    TCACCTGAGT CAAATGAGGA CATCTCTACA ACTGTAGTAT ATAGAATGGA AAGTCTGGGA
    GAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071432.1
    CDS180..1991
    Translation

    Target ORF information:

    RefSeq Version NM_001077964.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077964.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG CTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCATTC CTTTGGACCA
    TTGGACTATG AAAGTCTCCA ACAAGAACTT GCTTTAAAAG AGACAGTATG GAAAAAAGTG
    TCACCTGAGT CAAATGAGGA CATCTCTACA ACTGTAGTAT ATAGAATGGA AAGTCTGGGA
    GAGAAAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16525
    Clone ID Related Accession (Same CDS sequence) XM_005202579.2 , XM_005202579.3
    Accession Version XM_005202579.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103844.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGGTAAA GTCAGTGATA
    AACCTTTTGT TTGCTGCATA TACTGGAGAT GTGTCTGCAC TTCGAAGGTT TGCTTTGTCA
    GCCATGGACA TGGAACAGCG AGACTACGAT TCTAGAACGG CACTCCACGT AGCTGCTGCA
    GAGGGTCATG TTGAAGTTGT TAAATTTCTG CTGGAAGCCT GCAAAGTAAA TCCTTTCCCC
    AAGGACAGGT GGAATAACAC TCCCATGGAC GAAGCGCTGC ACTTTGGGCA CCACGACGTA
    TTTAAAATCC TGCAGGAATA CCAAGTCCAG TACACGCCTC AAGGAGATTC CGACAACGGA
    AAGGAGAATC AAACGGTCCA TAAGAATCTC GATGGACTGT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202636.1
    CDS260..2284
    Translation

    Target ORF information:

    RefSeq Version XM_005202579.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGGTAAA GTCAGTGATA
    AACCTTTTGT TTGCTGCATA TACTGGAGAT GTGTCTGCAC TTCGAAGGTT TGCTTTGTCA
    GCCATGGACA TGGAACAGCG AGACTACGAT TCTAGAACGG CACTCCACGT AGCTGCTGCA
    GAGGGTCATG TTGAAGTTGT TAAATTTCTG CTGGAAGCCT GCAAAGTAAA TCCTTTCCCC
    AAGGACAGGT GGAATAACAC TCCCATGGAC GAAGCGCTGC ACTTTGGGCA CCACGACGTA
    TTTAAAATCC TGCAGGAATA CCAAGTCCAG TACACGCCTC AAGGAGATTC CGACAACGGA
    AAGGAGAATC AAACGGTCCA TAAGAATCTC GATGGACTGT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16525
    Clone ID Related Accession (Same CDS sequence) XM_005202579.2 , XM_005202579.3
    Accession Version XM_005202579.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGGTAAA GTCAGTGATA
    AACCTTTTGT TTGCTGCATA TACTGGAGAT GTGTCTGCAC TTCGAAGGTT TGCTTTGTCA
    GCCATGGACA TGGAACAGCG AGACTACGAT TCTAGAACGG CACTCCACGT AGCTGCTGCA
    GAGGGTCATG TTGAAGTTGT TAAATTTCTG CTGGAAGCCT GCAAAGTAAA TCCTTTCCCC
    AAGGACAGGT GGAATAACAC TCCCATGGAC GAAGCGCTGC ACTTTGGGCA CCACGACGTA
    TTTAAAATCC TGCAGGAATA CCAAGTCCAG TACACGCCTC AAGGAGATTC CGACAACGGA
    AAGGAGAATC AAACGGTCCA TAAGAATCTC GATGGACTGT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202636.1
    CDS292..2316
    Translation

    Target ORF information:

    RefSeq Version XM_005202579.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202579.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGGTAAA GTCAGTGATA
    AACCTTTTGT TTGCTGCATA TACTGGAGAT GTGTCTGCAC TTCGAAGGTT TGCTTTGTCA
    GCCATGGACA TGGAACAGCG AGACTACGAT TCTAGAACGG CACTCCACGT AGCTGCTGCA
    GAGGGTCATG TTGAAGTTGT TAAATTTCTG CTGGAAGCCT GCAAAGTAAA TCCTTTCCCC
    AAGGACAGGT GGAATAACAC TCCCATGGAC GAAGCGCTGC ACTTTGGGCA CCACGACGTA
    TTTAAAATCC TGCAGGAATA CCAAGTCCAG TACACGCCTC AAGGAGATTC CGACAACGGA
    AAGGAGAATC AAACGGTCCA TAAGAATCTC GATGGACTGT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16526
    Clone ID Related Accession (Same CDS sequence) XM_005202580.3 , XM_005202580.4
    Accession Version XM_005202580.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103844.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCTTGG ACCTTTCTTC
    ACTCAAGTGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202637.1
    CDS260..1954
    Translation

    Target ORF information:

    RefSeq Version XM_005202580.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202580.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCTTGG ACCTTTCTTC
    ACTCAAGTGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16526
    Clone ID Related Accession (Same CDS sequence) XM_005202580.3 , XM_005202580.4
    Accession Version XM_005202580.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutaminase kidney isoform, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202580.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCTTGG ACCTTTCTTC
    ACTCAAGTGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202637.1
    CDS253..1947
    Translation

    Target ORF information:

    RefSeq Version XM_005202580.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glutaminase (GLS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202580.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCGAC TCCGAGGCTC GGCGATGCTG CGGGACCTGC TCTTGCGCCC GCCTGCTGCC 
    GCCTGCGCAG TTCTGAGGCG GGCGCAGCCT CTCGCCACCT TGTGCCGGCG ACCCCTGGGC
    GGGGGACGAA CGGGCCAGGT GGCCGCCGCG CGGCTCTACC CGTGGTGGGG CGGGGGCGGC
    CGGTCGGCGG GTCCCCTCAC GAAGGGCCTG TCCAGCTCGC CCTCGGAGAT CCTACAGGAG
    TTGGGCAAGG GGGCCACGCA GCAGCAGCAG CAGCAGCCGC CGCCGCAGCC TGGGGCGTCG
    CCGCCCGCAG TCCCGCAGGC GCCCGGCCCC AAGGACGGCC CGGGGGAGAC GGACGCGTTC
    TCCAACAACG AGGGCAAGGA GCTGGTGCCT TCAGGCGAAA ATAAAATAAA ACAGGGTCTG
    TTACCTAGTT TGGAAGATTT GCTGTTCTAT ACAATTGCAG AAGGACAAGA GAAAATACCT
    GTTCATAAAT TTATTACAGC ACTCAAATCT ACAGGATTGC GAACGTCTGA TCCCAGGTTG
    AAAGAGTGTA TGGATATGTT AAGATTAACT CTTCAAACAA CATCAGATGG TGTCATGCTA
    GACAAAGATC TTTTTAAAAA ATGTGTTCAA AGCAACATTG TTTTGTTGAC ACAAGCCTTT
    AGAAGAAAGT TTGTTATTCC TGACTTTATG TCTTTTACTT CACATATCGA TGAATTATAT
    GAGAGTGCTA AAAAGCAGTC TGGAGGAAAG GTTGCAGATT ATATTCCTCA ACTAGCCAAG
    TTCAGCCCTG ATTTGTGGGG TGTATCTGTT TGTACAGTAG ATGGACAGAG GCATTCTGTT
    GGAGATACCA AAGTTCCATT TTGTCTTCAG TCCTGTGTAA AGCCTTTGAA ATATGCCATT
    GCTGTTAATG ACCTTGGAAC AGAATATGTG CATCGATATG TTGGGAAAGA GCCAAGTGGA
    TTAAGGTTCA ACAAACTGTT TTTAAATGAA GATGATAAAC CACACAATCC TATGGTAAAT
    GCTGGAGCAA TTGTTGTGAC TTCATTAATA AAGCAAGGAG TGAATAATGC TGAAAAATTT
    GACTATGTAA TGCAGTTTTT AAATAAGATG GCTGGTAATG AATATGTTGG ATTCAGTAAT
    GCAACGTTTC AGTCTGAAAG AGAAAGTGGA GATCGAAATT TTGCAATAGG ATATTACTTA
    AAGGAAAAAA AGTGTTTCCC AGAAGGCACA GACATGGTTG GTATATTGGA CTTCTATTTC
    CAGCTGTGCT CCATCGAAGT AACTTGTGAG TCAGCTAGTG TGATGGCAGC AACACTGGCT
    AATGGTGGCT TCTGCCCAAT CACTGGTGAA AGAGTACTGA GTCCTGAAGC AGTTCGAAAT
    ACACTCAGTT TGATGCATTC CTGTGGCATG TATGATTTCT CAGGGCAGTT TGCTTTCCAT
    GTTGGTCTTC CTGCAAAATC TGGAGTTGCT GGAGGCATTC TTTTAGTTGT CCCCAATGTC
    ATGGGCATGA TGTGCTGGTC TCCTCCTCTG GACAAGATGG GCAACAGTGT TAAGGGAATT
    CACTTCTGTC ATGATCTTGT TTCTCTGTGT AATTTCCATA ACTATGATAA TTTGAGACAC
    TTTGCTAAAA AACTTGATCC TCGAAGAGAA GGTGGTGATC AGAGGCTTGG ACCTTTCTTC
    ACTCAAGTGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.