PLA2G4A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G4A
Entrez Gene ID	525072
Full Name	phospholipase A2 group IVA
General protein information	Preferred Names phospholipase A2 group IVA Names cytosolic phospholipase A2 cPLA2 phospholipase A2, group IVA (cytosolic, calcium-dependent)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976260.1	XP_024832028.1	cytosolic phospholipase A2 isoform X1
NM_001075864.1	NP_001069332.1	cytosolic phospholipase A2

Pathways from BioSystems

Homologies

	PLA2G4A	XP_001107479.2
Bos taurus (cattle)	PLA2G4A	NP_001069332.1
Gallus gallus (chicken)	PLA2G4A	NP_990754.1
Homo sapiens (human)	PLA2G4A	NP_077734.1
Xenopus tropicalis (tropical clawed frog)	pla2g4a	NP_001120577.1
Pan troglodytes (chimpanzee)	PLA2G4A	XP_001165694.1
Rattus norvegicus (Norway rat)	Pla2g4a	NP_598235.2
Danio rerio (zebrafish)	cpla2	NP_571370.1
Canis lupus familiaris (dog)	PLA2G4A	XP_005622510.1
Mus musculus (house mouse)	Pla2g4a	NP_032895.1
Danio rerio (zebrafish)	pla2g4a	XP_687485.3

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a 42-kDa Group IV cPLA2-activating protein, cPLAP?, as a GTP-binding protein in the bovine brain.
Bach JH, Jung KM, Choi JS, Jung SY, Chin MR, Ahn KH, Kim SK, Kim DK
Journal of biochemistry150(4)385-94(2011 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Activation of cytosolic phospholipase A2 and fatty acid transacylase is essential but not sufficient for thrombin-induced smooth muscle cell proliferation.
Gluck N, Schwob O, Krimsky M, Yedgar S
American journal of physiology. Cell physiology294(6)C1597-603(2008 Jun)

Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulosa cells of ovulatory follicles.
Diouf MN, Sayasith K, Lefebvre R, Silversides DW, Sirois J, Lussier JG
Biology of reproduction74(6)1096-103(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

result suggests that cPLAPgamma may act as a G protein, activating cPLA(2)alpha prior to reaching full intracellular Ca(2 ) concentrations
Title: Identification of a 42-kDa Group IV cPLA2-activating protein, cPLAP?, as a GTP-binding protein in the bovine brain.

Activation of cytosolic phospholipase A2 and fatty acid transacylase is essential but not sufficient for thrombin-induced smooth muscle cell proliferation.
Title: Activation of cytosolic phospholipase A2 and fatty acid transacylase is essential but not sufficient for thrombin-induced smooth muscle cell proliferation.

Ovulation is associated with an LH/hCG-dependent induction of PLA2G4A in GC via the adenylyl cyclase/cAMP pathway.
Title: Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulosa cells of ovulatory follicles.

The following PLA2G4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43637	NM_001075864.1 Latest version!	Bos taurus phospholipase A2 group IVA (PLA2G4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa223597	XM_024976260.1 Latest version!	Bos taurus phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G4A ( NM_001075864.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069332.1 Bos taurus phospholipase A2 group IVA (PLA2G4A), mRNA.

Price & Availability↑

CloneID	OBa43637
Clone ID Related Accession (Same CDS sequence)	NM_001075864.1
Accession Version	NM_001075864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	cytosolic phospholipase A2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY363688.1. On or before Jan 4, 2015 this sequence version replaced XM_010823244.1, XM_005194904.1, XM_603415.2. ##Evidence-Data-START## Transcript exon combination :: AY363688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145414, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGTCATTTA TAGATCCTTA TCAACACATT ATAGTGGAGC ACCATTATTC CCACAAGTTC 
ACAGTAGTGG TGCTGCGTGC CACAAAAGTG ACAAAGGGAA CCTTTGGTGA CATGCTTGAC 
ACTCCAGACC CCTATGTGGA ACTTTTCATC TCTTCAACCC CCGACAGCAG GAAGAGAACA 
AGACACTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATATTTT GGAGATCACT TTAATGGATG CCAATTATGT TATGGATGAA 
ACTCTTGGGA CGACAACGTT TCCCATATCT TCTATGAAAG TGGGAGAGAA GAAACAGGTT 
CCTTTTATTT TCAATCAAGT CACTGAAATG ATTCTGGAAA TGTCTCTTGA AGTTTGTTCA 
TCCCCAGACC TCCGGTTTAG TATGGCTCTG TGTGACCAGG AGAAAGCTTT CAGACAACAG 
AGAAAGGAAA ACATAAAGGA AAACATGAAG AAACTGCTGG GTCCAAAGAA TAGTGAAGGC 
TTGCATTCTA CACGTGATGT GCCTGTGGTG GCCATACTGG GCTCAGGTGG GGGATTTCGA 
GCCATGGTAG GATTCTCTGG TGTGATGAAG GCACTGTATG AGTCAGGGAT TTTGGACTGT 
GCTACCTACA TTGCTGGTCT TTCTGGATCC ACATGGTATA TGTCGACCTT ATATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAACAAAG AGCTAATGAA AAATGTCAGC 
CACAACCCTC TTTTACTTCT CACACCACAG AAAATTAAAA GATATGTTGA GTCTTTATGG 
AGGAAAAAAA GTTCTGGACA ACCTGTCACC TTTACTGATA TCTTCGGGAT GTTAATAGGA 
GAAACACTAA TTCACAATAG AATGAACACT ACCCTGAGTA GTTTGAAGGA AAAAGTCAAT 
ACTGGGCAGT GTCCTCTGCC GCTTTTCACC TGTCTCCATG TCAAACCCGA TGTCTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATTTGAGA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CGCCTGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT TGTAAAGAAA 
TACGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GTAGTGCCTT TTCTATATTG 
TTCAACAGAG TCTTGGGAGT TTCTGGATCA CAAAGTAAAG GTTCCACAAT GGAAGAAGAA 
CTAGAAAATA TTACAGCAAA GCATATTGTG AGTAATGATA GCTCAGACAG TGATGACGAA 
TCACAAGGAC CCAAAGGCAC TGAACATGAA GAAGCAGAAA GGGAATACCA AAATGATAAT 
CAAGCAAGTT GGGTCCAACG TATGCTCATG GCCTTGGTGA GTGACTCAGC TTTGTTCAAT 
ACCAGAGAAG GACGTGCCGG GAAGGTGCAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCCTATCCAA TGTCTCCTTT GAGAGACTTT ACCATGCAAG AGTCCCTGGA TGAAGACGAA 
TTGGATGCCG CTGTCGCAGA TCCTGATGAA TTTGAGCAGA TATATGAGCC TTTGGATGTC 
AAAAGTAAAA AGATTCATGT GGTGGACAGT GGCCTCACAT TTAACCTTCC ATACCCTTTG 
ATCCTGAGAC CTCAGAGGGG AGTTGATCTC ATCATCTCCT TTGACTTTTC TGCAAGACCA 
GGTGACTCCA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAC GTATTCGATC GAGAAGGACT GAAGGAATGC 
TATGTTTTTA AACCCAAGAA TCCTGATGTC GAGAAAGATT GCCCGACTAT CATTCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG AGAAACTAAT 
GAAGAGAAAG AAATAGCTGA TTTTGATATT TTTGATGACC CTGAATCACC GTTTTCGACC 
TTCAACTTTC AATATCCCAA TCAAGCATTT AAAAGGCTGC ATGATCTCAT GTACTTCAAT 
ACCCTGAACA ACATTGATGT AATAAAGAAT GCTATAGTCG AAAGCATCGA ATATAGAAGG 
CAGAATCCGT CTCGTTGCTC TGTTTCCCTC AGTAGTGTTG AGGCAAGAAG ATTCTTCAAC 
AAGGAGTTTC TAAGCAAACC CACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069332.1
CDS	141..2390
Translation	MSFIDPYQHIIVEHHYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISSTPDSRKRTRHFNNDINPVWNETFEFILDPNQENILEITLMDANYVMDETLGTTTFPISSMKVGEKKQVPFIFNQVTEMILEMSLEVCSSPDLRFSMALCDQEKAFRQQRKENIKENMKKLLGPKNSEGLHSTRDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYIAGLSGSTWYMSTLYSHPDFPEKGPEEINKELMKNVSHNPLLLLTPQKIKRYVESLWRKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTGQCPLPLFTCLHVKPDVSELMFADWVEFSPFEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSKGSTMEEELENITAKHIVSNDSSDSDDESQGPKGTEHEEAEREYQNDNQASWVQRMLMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPMSPLRDFTMQESLDEDELDAAVADPDEFEQIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPGDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDVEKDCPTIIHFVLANINFRKYKAPGVPRETNEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFNTLNNIDVIKNAIVESIEYRRQNPSRCSVSLSSVEARRFFNKEFLSKPTA

Target ORF information:

RefSeq Version	NM_001075864.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase A2 group IVA (PLA2G4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075864.1

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA TCAACACATT ATAGTGGAGC ACCATTATTC CCACAAGTTC 
ACAGTAGTGG TGCTGCGTGC CACAAAAGTG ACAAAGGGAA CCTTTGGTGA CATGCTTGAC 
ACTCCAGACC CCTATGTGGA ACTTTTCATC TCTTCAACCC CCGACAGCAG GAAGAGAACA 
AGACACTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATATTTT GGAGATCACT TTAATGGATG CCAATTATGT TATGGATGAA 
ACTCTTGGGA CGACAACGTT TCCCATATCT TCTATGAAAG TGGGAGAGAA GAAACAGGTT 
CCTTTTATTT TCAATCAAGT CACTGAAATG ATTCTGGAAA TGTCTCTTGA AGTTTGTTCA 
TCCCCAGACC TCCGGTTTAG TATGGCTCTG TGTGACCAGG AGAAAGCTTT CAGACAACAG 
AGAAAGGAAA ACATAAAGGA AAACATGAAG AAACTGCTGG GTCCAAAGAA TAGTGAAGGC 
TTGCATTCTA CACGTGATGT GCCTGTGGTG GCCATACTGG GCTCAGGTGG GGGATTTCGA 
GCCATGGTAG GATTCTCTGG TGTGATGAAG GCACTGTATG AGTCAGGGAT TTTGGACTGT 
GCTACCTACA TTGCTGGTCT TTCTGGATCC ACATGGTATA TGTCGACCTT ATATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAACAAAG AGCTAATGAA AAATGTCAGC 
CACAACCCTC TTTTACTTCT CACACCACAG AAAATTAAAA GATATGTTGA GTCTTTATGG 
AGGAAAAAAA GTTCTGGACA ACCTGTCACC TTTACTGATA TCTTCGGGAT GTTAATAGGA 
GAAACACTAA TTCACAATAG AATGAACACT ACCCTGAGTA GTTTGAAGGA AAAAGTCAAT 
ACTGGGCAGT GTCCTCTGCC GCTTTTCACC TGTCTCCATG TCAAACCCGA TGTCTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATTTGAGA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CGCCTGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT TGTAAAGAAA 
TACGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GTAGTGCCTT TTCTATATTG 
TTCAACAGAG TCTTGGGAGT TTCTGGATCA CAAAGTAAAG GTTCCACAAT GGAAGAAGAA 
CTAGAAAATA TTACAGCAAA GCATATTGTG AGTAATGATA GCTCAGACAG TGATGACGAA 
TCACAAGGAC CCAAAGGCAC TGAACATGAA GAAGCAGAAA GGGAATACCA AAATGATAAT 
CAAGCAAGTT GGGTCCAACG TATGCTCATG GCCTTGGTGA GTGACTCAGC TTTGTTCAAT 
ACCAGAGAAG GACGTGCCGG GAAGGTGCAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCCTATCCAA TGTCTCCTTT GAGAGACTTT ACCATGCAAG AGTCCCTGGA TGAAGACGAA 
TTGGATGCCG CTGTCGCAGA TCCTGATGAA TTTGAGCAGA TATATGAGCC TTTGGATGTC 
AAAAGTAAAA AGATTCATGT GGTGGACAGT GGCCTCACAT TTAACCTTCC ATACCCTTTG 
ATCCTGAGAC CTCAGAGGGG AGTTGATCTC ATCATCTCCT TTGACTTTTC TGCAAGACCA 
GGTGACTCCA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAC GTATTCGATC GAGAAGGACT GAAGGAATGC 
TATGTTTTTA AACCCAAGAA TCCTGATGTC GAGAAAGATT GCCCGACTAT CATTCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG AGAAACTAAT 
GAAGAGAAAG AAATAGCTGA TTTTGATATT TTTGATGACC CTGAATCACC GTTTTCGACC 
TTCAACTTTC AATATCCCAA TCAAGCATTT AAAAGGCTGC ATGATCTCAT GTACTTCAAT 
ACCCTGAACA ACATTGATGT AATAAAGAAT GCTATAGTCG AAAGCATCGA ATATAGAAGG 
CAGAATCCGT CTCGTTGCTC TGTTTCCCTC AGTAGTGTTG AGGCAAGAAG ATTCTTCAAC 
AAGGAGTTTC TAAGCAAACC CACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4A ( XM_024976260.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832028.1 Bos taurus phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223597
Clone ID Related Accession (Same CDS sequence)	XM_024976260.1
Accession Version	XM_024976260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytosolic phospholipase A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTTA TAGATCCTTA TCAACACATT ATAGTGGAGC ACCATTATTC CCACAAGTTC 
ACAGTAGTGG TGCTGCGTGC CACAAAAGTG ACAAAGGGAA CCTTTGGTGA CATGCTTGAC 
ACTCCAGACC CCTATGTGGA ACTTTTCATC TCTTCAACCC CCGACAGCAG GAAGAGAACA 
AGACACTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATATTTT GGAGATCACT TTAATGGATG CCAATTATGT TATGGATGAA 
ACTCTTGGGA CGACAACGTT TCCCATATCT TCTATGAAAG TGGGAGAGAA GAAACAGGTT 
CCTTTTATTT TCAATCAAGT CACTGAAATG ATTCTGGAAA TGTCTCTTGA AGTTTGTTCA 
TCCCCAGACC TCCGGTTTAG TATGGCTCTG TGTGACCAGG AGAAAGCTTT CAGACAACAG 
AGAAAGGAAA ACATAAAGGA AAACATGAAG AAACTGCTGG GTCCAAAGAA TAGTGAAGGC 
TTGCATTCTA CACGTGATGT GCCTGTGGTG GCCATACTGG GCTCAGGTGG GGGATTTCGA 
GCCATGGTAG GATTCTCTGG TGTGATGAAG GCACTGTATG AGTCAGGGAT TTTGGACTGT 
GCTACCTACA TTGCTGGTCT TTCTGGATCC ACATGGTATA TGTCGACCTT ATATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAACAAAG AGCTAATGAA AAATGTCAGC 
CACAACCCTC TTTTACTTCT CACACCACAG AAAATTAAAA GATATGTTGA GTCTTTATGG 
AGGAAAAAAA GTTCTGGACA ACCTGTCACC TTTACTGATA TCTTCGGGAT GTTAATAGGA 
GAAACACTAA TTCACAATAG AATGAACACT ACCCTGAGTA GTTTGAAGGA AAAAGTCAAT 
ACTGGGCAGT GTCCTCTGCC GCTTTTCACC TGTCTCCATG TCAAACCCGA TGTCTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATTTGAGA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CGCCTGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT TGTAAAGAAA 
TACGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GTAGTGCCTT TTCTATATTG 
TTCAACAGAG TCTTGGGAGT TTCTGGATCA CAAAGTAAAG GTTCCACAAT GGAAGAAGAA 
CTAGAAAATA TTACAGCAAA GCATATTGTG AGTAATGATA GCTCAGACAG TGATGACGAA 
TCACAAGGAC CCAAAGGCAC TGAACATGAA GAAGCAGAAA GGGAATACCA AAATGATAAT 
CAAGCAAGTT GGGTCCAACG TATGCTCATG GCCTTGGTGA GTGACTCAGC TTTGTTCAAT 
ACCAGAGAAG GACGTGCCGG GAAGGTGCAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCCTATCCAA TGTCTCCTTT GAGAGACTTT ACCATGCAAG AGTCCCTGGA TGAAGACGAA 
TTGGATGCCG CGGTCGCAGA TCCTGATGAA TTTGAGCAGA TATATGAGCC TTTGGATGTC 
AAAAGTAAAA AGATTCATGT GGTGGACAGT GGCCTCACAT TTAACCTTCC ATACCCTTTG 
ATCCTGAGAC CTCAGAGGGG AGTTGATCTC ATCATCTCCT TTGACTTTTC TGCAAGACCA 
AGTGACTCCA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAC GTATTCGATC GAGAAGGACT GAAGGAATGC 
TATGTTTTTA AACCCAAGAA TCCTGATGTC GAGAAAGATT GCCCGACTAT CATTCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG AGAAACTAAT 
GAAGAGAAAG AAATAGCTGA TTTTGATATT TTTGATGACC CTGAATCACC GTTTTCGACC 
TTCAACTTTC AATATCCCAA TCAAGCATTT AAAAGGCTGC ATGATCTCAT GTACTTCAAT 
ACCCTGAACA ACATTGATGT AATAAAGAAT GCTATAGTCG AAAGCATCGA ATATAGAAGG 
CAGAATCCGT CTCGTTGCTC TGTTTCCCTC AGTAGTGTTG AGGCAAGAAG ATTCTTCAAC 
AAGGAGTTTC TAAGCAAACC CACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832028.1
CDS	90..2339
Translation	MSFIDPYQHIIVEHHYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISSTPDSRKRTRHFNNDINPVWNETFEFILDPNQENILEITLMDANYVMDETLGTTTFPISSMKVGEKKQVPFIFNQVTEMILEMSLEVCSSPDLRFSMALCDQEKAFRQQRKENIKENMKKLLGPKNSEGLHSTRDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYIAGLSGSTWYMSTLYSHPDFPEKGPEEINKELMKNVSHNPLLLLTPQKIKRYVESLWRKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTGQCPLPLFTCLHVKPDVSELMFADWVEFSPFEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSKGSTMEEELENITAKHIVSNDSSDSDDESQGPKGTEHEEAEREYQNDNQASWVQRMLMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPMSPLRDFTMQESLDEDELDAAVADPDEFEQIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDVEKDCPTIIHFVLANINFRKYKAPGVPRETNEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFNTLNNIDVIKNAIVESIEYRRQNPSRCSVSLSSVEARRFFNKEFLSKPTA

Target ORF information:

RefSeq Version	XM_024976260.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase A2 group IVA (PLA2G4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976260.1

ORF Insert Sequence:

ATGTCATTTA TAGATCCTTA TCAACACATT ATAGTGGAGC ACCATTATTC CCACAAGTTC 
ACAGTAGTGG TGCTGCGTGC CACAAAAGTG ACAAAGGGAA CCTTTGGTGA CATGCTTGAC 
ACTCCAGACC CCTATGTGGA ACTTTTCATC TCTTCAACCC CCGACAGCAG GAAGAGAACA 
AGACACTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT 
CCTAATCAGG AAAATATTTT GGAGATCACT TTAATGGATG CCAATTATGT TATGGATGAA 
ACTCTTGGGA CGACAACGTT TCCCATATCT TCTATGAAAG TGGGAGAGAA GAAACAGGTT 
CCTTTTATTT TCAATCAAGT CACTGAAATG ATTCTGGAAA TGTCTCTTGA AGTTTGTTCA 
TCCCCAGACC TCCGGTTTAG TATGGCTCTG TGTGACCAGG AGAAAGCTTT CAGACAACAG 
AGAAAGGAAA ACATAAAGGA AAACATGAAG AAACTGCTGG GTCCAAAGAA TAGTGAAGGC 
TTGCATTCTA CACGTGATGT GCCTGTGGTG GCCATACTGG GCTCAGGTGG GGGATTTCGA 
GCCATGGTAG GATTCTCTGG TGTGATGAAG GCACTGTATG AGTCAGGGAT TTTGGACTGT 
GCTACCTACA TTGCTGGTCT TTCTGGATCC ACATGGTATA TGTCGACCTT ATATTCTCAC 
CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAACAAAG AGCTAATGAA AAATGTCAGC 
CACAACCCTC TTTTACTTCT CACACCACAG AAAATTAAAA GATATGTTGA GTCTTTATGG 
AGGAAAAAAA GTTCTGGACA ACCTGTCACC TTTACTGATA TCTTCGGGAT GTTAATAGGA 
GAAACACTAA TTCACAATAG AATGAACACT ACCCTGAGTA GTTTGAAGGA AAAAGTCAAT 
ACTGGGCAGT GTCCTCTGCC GCTTTTCACC TGTCTCCATG TCAAACCCGA TGTCTCAGAG 
CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATTTGAGA TTGGCATGGC TAAATATGGT 
ACTTTTATGG CGCCTGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT TGTAAAGAAA 
TACGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GTAGTGCCTT TTCTATATTG 
TTCAACAGAG TCTTGGGAGT TTCTGGATCA CAAAGTAAAG GTTCCACAAT GGAAGAAGAA 
CTAGAAAATA TTACAGCAAA GCATATTGTG AGTAATGATA GCTCAGACAG TGATGACGAA 
TCACAAGGAC CCAAAGGCAC TGAACATGAA GAAGCAGAAA GGGAATACCA AAATGATAAT 
CAAGCAAGTT GGGTCCAACG TATGCTCATG GCCTTGGTGA GTGACTCAGC TTTGTTCAAT 
ACCAGAGAAG GACGTGCCGG GAAGGTGCAC AACTTCATGC TGGGCTTGAA TCTCAATACA 
TCCTATCCAA TGTCTCCTTT GAGAGACTTT ACCATGCAAG AGTCCCTGGA TGAAGACGAA 
TTGGATGCCG CGGTCGCAGA TCCTGATGAA TTTGAGCAGA TATATGAGCC TTTGGATGTC 
AAAAGTAAAA AGATTCATGT GGTGGACAGT GGCCTCACAT TTAACCTTCC ATACCCTTTG 
ATCCTGAGAC CTCAGAGGGG AGTTGATCTC ATCATCTCCT TTGACTTTTC TGCAAGACCA 
AGTGACTCCA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC 
AAGCTCCCCT TTCCAAAGAT TGATCCTTAC GTATTCGATC GAGAAGGACT GAAGGAATGC 
TATGTTTTTA AACCCAAGAA TCCTGATGTC GAGAAAGATT GCCCGACTAT CATTCACTTT 
GTTCTGGCCA ACATCAACTT CAGAAAGTAC AAGGCTCCAG GTGTTCCAAG AGAAACTAAT 
GAAGAGAAAG AAATAGCTGA TTTTGATATT TTTGATGACC CTGAATCACC GTTTTCGACC 
TTCAACTTTC AATATCCCAA TCAAGCATTT AAAAGGCTGC ATGATCTCAT GTACTTCAAT 
ACCCTGAACA ACATTGATGT AATAAAGAAT GCTATAGTCG AAAGCATCGA ATATAGAAGG 
CAGAATCCGT CTCGTTGCTC TGTTTCCCTC AGTAGTGTTG AGGCAAGAAG ATTCTTCAAC 
AAGGAGTTTC TAAGCAAACC CACAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a 42-kDa Group IV cPLA2-activating protein, cPLAP?, as a GTP-binding protein in the bovine brain.
Journal of biochemistry150(4)385-94(2011 Oct)
Bach JH,Jung KM,Choi JS,Jung SY,Chin MR,Ahn KH,Kim SK,Kim DK

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Activation of cytosolic phospholipase A2 and fatty acid transacylase is essential but not sufficient for thrombin-induced smooth muscle cell proliferation.
American journal of physiology. Cell physiology294(6)C1597-603(2008 Jun)
Gluck N,Schwob O,Krimsky M,Yedgar S

Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulosa cells of ovulatory follicles.
Biology of reproduction74(6)1096-103(2006 Jun)
Diouf MN,Sayasith K,Lefebvre R,Silversides DW,Sirois J,Lussier JG