ARHGEF18 cDNA ORF clone, Bos taurus(cattle)

The following ARHGEF18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50572 XM_010798248.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa50572 XM_010806873.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa236470 XM_024994912.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa90243 XM_015464087.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa90243 XM_015472030.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa90244 XM_015472031.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90244 XM_015464088.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa50571 XM_010806870.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010806872.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010798246.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010798247.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010798245.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010806871.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010806868.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010798244.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa50571 XM_010806868.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50572 XM_010806873.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa50571 XM_010806871.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010806872.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa50571 XM_010806870.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa90244 XM_015472031.1 Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50572
    Clone ID Related Accession (Same CDS sequence) XM_010798248.2 , XM_010806873.2 , XM_010806873.3
    Accession Version XM_010798248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796550.1
    CDS1059..4037
    Translation

    Target ORF information:

    RefSeq Version XM_010798248.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50572
    Clone ID Related Accession (Same CDS sequence) XM_010798248.2 , XM_010806873.2 , XM_010806873.3
    Accession Version XM_010806873.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805175.1
    CDS1059..4037
    Translation

    Target ORF information:

    RefSeq Version XM_010806873.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50572
    Clone ID Related Accession (Same CDS sequence) XM_010798248.2 , XM_010806873.2 , XM_010806873.3
    Accession Version XM_010806873.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805175.1
    CDS1009..3987
    Translation

    Target ORF information:

    RefSeq Version XM_010806873.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806873.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACGGTCT CGCAGAAGGG GGGTCCACAG CCAGCACCCA GCCCAGCGGG AACCGGGACA 
    AGACTCGGAC CACTCACAGG AGAGATGGAT GAAACTGATA CACTGTTTCC GAAGCTCAAG
    CAGGTGGCCG ATGACTCGCT TTTTCTTACA TCTTCCAGCA CTGAGCCCAT CTTTATAGAA
    GACCCCTACA CTGCCTCGCT GAGAGGCGAG GTCGAAGCGG ACACCCACGA GTTCGAGGCT
    GAGTCCTGGA GCCTCTCTGT GGACCCCGAG TATGTGAAGA AACAGAAGAG GGAGGTGGTG
    AAAAGGCAGG ACGTGCTTTA TGAGCTGATG CAGACGGAGG CCCACCATGT GCGGACGCTC
    AAGATCATGC TCAAGGTCTA CTCCCGGGCC CTGCGGGAGG AGCTGCAATT CAGCCCCAAG
    GCCGTCAGCC GCCTGTTCCC AGGCGCAGAC GACCTGCTGG AGGTGCACAG CCACTTCCTG
    TGTCGGCTGA AGGAGCGCCG CCTGGAGTCG CTGGAGGAGG GCAGTGCCCA CAACTACATC
    ATCCGGAAGA TCGGGGACCT CCTGCTTCAG CAGTTTTCAG GTGAAAACGG AGAGAGGATG
    AAAGAAAAAT ACAGCGTCTT TTGTAGCGGT CACAACGATG CTGTCAGTCA CTACAAGTTG
    CTGCTGCAGC AAAACAAGAA ATTTCAAAAC TTGATCAAGA GAATCGGCAA CTTCTCCATC
    GTGAGGCGGC TCGGCGTGCA GGAGTGTATC CTCCTGGTCA CCCAACGCAT CACCAAATAC
    CCTGTGCTGG TAGAGCGAAT TATCCAGAAT ACAGAAGCTG GCTCCGAGGA CCACAGAGAC
    CTGACCCAGG CCCTGAACCT CATCAAAGAC ATCATCTCAC AAGTGGACGC CAGGGTCAGT
    GAGTGCGAGA AGGGCCAGCG CCTCAGGGAG ATCGCTGGGA AGATGGACCT CAAGTCCTCC
    AGCAAGCTCA AAAATGGGCT GACCTTCCGC AAGGAGGACA TGCTGCAGAG GCAGCTCCAC
    CTGGAGGGCA CCCTGTGCTG GAAGACCACC TCTGGGCGCC TGAAAGACGT CCTCGCAGTC
    CTGTTGACAG ATGTGCTGCT GCTGCTCCAA GAAAAGGATC AGAAATACGT CTTTGCATCC
    GTGGACTCCA AGCGCCCCGT CATCTCGCTG CAAAAGCTCA TCGTGAGGGA GGTAGCCAAC
    GAGGAGAAAG CCATGTTTCT GATCAGTGCC TCCCTGCATG GGCCCGAGAT GTACGAGATC
    TATACCAGCT CGAAGGAAGA CAGGAACACC TGGATGGCCC ACATCCGGAG GGCCGTGGAG
    AGCTGCCCAG ACGAAGAGGA AGGGCCCTTC AGCGAGGCCG AAGAGGAAAA GAAGGTGGTC
    GAGACGCGTG CTGTGAGGCT GAGGGACTTC CAAGAGCGAC TGAACAGGAA AGACCAGCAG
    ATTGCACAGA GCCTGAGCGA GAAGCAGCAG ATCTACCTGG AGATGGCAGA GATGAGCGGG
    CTCGAGGACC TCGCCCTGTC TCGGCTTCTC TTCCGGGGAG GGGACCCCCC CGAGAACCTG
    CAGGGCGAGC TGATTCTCAA GTCAGCCATG AGTGAGATCG AAGACATCCA GAGTTTCATC
    TGCAGGCGGC TGGGCAACGC CTACTGCCAG GCAGAAGACG GGGGCAGCGC AGGCAGCCCA
    GGCAAGGACG GCAGTCTTCA GAAGAACATG TGCAGCAGTG ACCTCAGCCC CCGAGACTGG
    CAGGGCCTGG TGAGCAGCCT GGACTCGAGG CCTGGAGACA CCCAGGGGGC CCCCGAGGAA
    GCCTCAAAGG TGGCAGAGTC ACCAGGTGCA GACCCCGGCC CCCACCCACC TGCGGTCCTG
    GAGTTGGAGC TCGTCCAGCG GATCCAGACG CTGTCCCAGC TGCTCCTCAG CCTCCAGGTG
    GTCATCGCCC AGCAGGACAG CTACGTGGAA ATTCAGAAAG CAGCCATCCA GGAGCGCGAG
    AAGCAGTTCC GGCTGCAGTC AACACGCGGG AACCTGCTGC TGGAACAGGA GCGGCAGCGC
    AACTTTGAGA AGCAGCGAGA GGAGCTGGCA GGCGTGCAGA AGCTGCAGGG CCAGCTGCGG
    CTGGAGCAGC AACGCTGGGA GCGCGAGCGG GAGCAGCAGC GCCGGGAGCT GGACCAGGCC
    TCGGCGCGGC TGCAGCAGCG TGAGAGTGAG TTGCTGCGGC TGCAGGAGCG GCTGAACCAG
    GAGCGCGAGG AGCTGGAGCG CCAGCGCCAG GCCTACCAGC ATGACCTGGA GCGGCTGCGC
    GAGGCCCAGC GCGCCGTGGA GCGGGAGCGC GAGCGCCTAG AGGTGCTGCG CCGGCTCAAG
    AAGCAGAACA CAGCGCCTGG GGCGCTGCCG CCGGAGGCAC TGGCGGAGAC CCAGCCTCTA
    AGCCACACTC CCAGCTTCAA TGGGAATGGG CTGGATGGAT CCATGACCCT GACCAAAGCA
    CCAGGGCCCC GACCCACCTC GCTGGTGTCC AGTGCGGGGT CCGAGTATGC CGAGCGCCCC
    GAGGTGGCCC GCAGGGACAG CGCCCCGGCC AAGAGCGAAG TGCCCATCCA GCTGCTGAGT
    GCCACCAACC AGATCCAGAG GCAGGCAGCT GTGCAGCAGC AGATTCCCAC CAAGTTGGCA
    GCCTCCACCA AGGGCGGAAA GGACAAGGGA GGCAAGAACC GGGGCTCCCA GCGCTGGGAC
    AGCTCGGCCT CGTTTGACCT GAGGCAGCCG CTGCTTCTCA ACAAGCTCAT GGGCAAAGAA
    GAGAACACCT CTCGGAACCG CCGCTCGCTG AGTCCCGTCC TGCCCAGCTC CACGCCCCAC
    CAAGACCCCA GCCTTCCAGC CCCGACCCCG GGCCCAGCCG ATGTCCCCAC CGAGTCCCTC
    CCCCTCAAGA CCTGGGGGCC ATCCTTTCCA CCCGGGGCCC CGTCCTCCAC ACCAGTCAAC
    AAGGAAGAAA CGGGCAAGGA AGACGTCATC TTCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236470
    Clone ID Related Accession (Same CDS sequence) XM_024994912.1
    Accession Version XM_024994912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT TCGAACCTCA 
    CCTGGTTTGA GTTCTTGTCT GACATCCCTG AAGGAGCACC CGAGGGCCAC CCTCCTGTCT
    GATGGCAGCC CACGCCCGAC CAGGAATGTC GGCATGACGG TCTCGCAGAA GGGGGGTCCA
    CAGCCAGCAC CCAGCCCAGC GGGAACCGGG ACAAGACTCG GACCACTCAC AGGAGAGATG
    GATGAAACTG ATACACTGTT TCCGAAGCTC AAGCAGGTGG CCGATGACTC GCTTTTTCTT
    ACATCTTCCA GCACTGAGCC CATCTTTATA GAAGACCCCT ACACTGCCTC GCTGAGAGGC
    GAGGTCGAAG CGGACACCCA CGAGTTCGAG GCTGAGTCCT GGAGCCTCTC TGTGGACCCC
    GAGTATGTGA AGAAACAGAA GAGGGAGGTG GTGAAAAGGC AGGACGTGCT TTATGAGCTG
    ATGCAGACGG AGGCCCACCA TGTGCGGACG CTCAAGATCA TGCTCAAGGT CTACTCCCGG
    GCCCTGCGGG AGGAGCTGCA ATTCAGCCCC AAGGCCGTCA GCCGCCTGTT CCCAGGCGCA
    GACGACCTGC TGGAGGTGCA CAGCCACTTC CTGTGTCGGC TGAAGGAGCG CCGCCTGGAG
    TCGCTGGAGG AGGGCAGTGC CCACAACTAC ATCATCCGGA AGATCGGGGA CCTCCTGCTT
    CAGCAGTTTT CAGGTGAAAA CGGAGAGAGG ATGAAAGAAA AATACAGCGT CTTTTGTAGC
    GGTCACAACG ATGCTGTCAG TCACTACAAG TTGCTGCTGC AGCAAAACAA GAAATTTCAA
    AACTTGATCA AGAGAATCGG CAACTTCTCC ATCGTGAGGC GGCTCGGCGT GCAGGAGTGT
    ATCCTCCTGG TCACCCAACG CATCACCAAA TACCCTGTGC TGGTAGAGCG AATTATCCAG
    AATACAGAAG CTGGCTCCGA GGACCACAGA GACCTGACCC AGGCCCTGAA CCTCATCAAA
    GACATCATCT CACAAGTGGA CGCCAGGGTC AGTGAGTGCG AGAAGGGCCA GCGCCTCAGG
    GAGATCGCTG GGAAGATGGA CCTCAAGTCC TCCAGCAAGC TCAAAAATGG GCTGACCTTC
    CGCAAGGAGG ACATGCTGCA GAGGCAGCTC CACCTGGAGG GCACCCTGTG CTGGAAGACC
    ACCTCTGGGC GCCTGAAAGA CGTCCTCGCA GTCCTGTTGA CAGATGTGCT GCTGCTGCTC
    CAAGAAAAGG ATCAGAAATA CGTCTTTGCA TCCGTGGACT CCAAGCGCCC CGTCATCTCG
    CTGCAAAAGC TCATCGTGAG GGAGGTAGCC AACGAGGAGA AAGCCATGTT TCTGATCAGT
    GCCTCCCTGC ATGGGCCCGA GATGTACGAG ATCTATACCA GCTCGAAGGA AGACAGGAAC
    ACCTGGATGG CCCACATCCG GAGGGCCGTG GAGAGCTGCC CAGACGAAGA GGAAGGGCCC
    TTCAGCGAGG CCGAAGAGGA AAAGAAGGTG GTCGAGACGC GTGCTGTGAG GCTGAGGGAC
    TTCCAAGAGC GACTGAACAG GAAAGACCAG CAGATTGCAC AGAGCCTGAG CGAGAAGCAG
    CAGATCTACC TGGAGATGGC AGAGATGAGC GGGCTCGAGG ACCTCGCCCT GTCTCGGCTT
    CTCTTCCGGG GAGGGGACCC CCCCGAGAAC CTGCAGGGCG AGCTGATTCT CAAGTCAGCC
    ATGAGTGAGA TCGAAGACAT CCAGAGTTTC ATCTGCAGGC GGCTGGGCAA CGCCTACTGC
    CAGGCAGAAG ACGGGGGCAG CGCAGGCAGC CCAGGCAAGG ACGGCAGTCT TCAGAAGAAC
    ATGTGCAGCA GTGACCTCAG CCCCCGAGAC TGGCAGGGCC TGGTGAGCAG CCTGGACTCG
    AGGCCTGGAG ACACCCAGGG GGCCCCCGAG GAAGCCTCAA AGGTGGCAGA GTCACCAGGT
    GCAGACCCCG GCCCCCACCC ACCTGCGGTC CTGGAGTTGG AGCTCGTCCA GCGGATCCAG
    ACGCTGTCCC AGCTGCTCCT CAGCCTCCAG GTGGTCATCG CCCAGCAGGA CAGCTACGTG
    GAAATTCAGA AAGCAGCCAT CCAGGAGCGC GAGAAGCAGT TCCGGCTGCA GTCAACACGC
    GGGAACCTGC TGCTGGAACA GGAGCGGCAG CGCAACTTTG AGAAGCAGCG AGAGGAGCTG
    GCAGGCGTGC AGAAGCTGCA GGGCCAGCTG CGGCTGGAGC AGCAACGCTG GGAGCGCGAG
    CGGGAGCAGC AGCGCCGGGA GCTGGACCAG GCCTCGGCGC GGCTGCAGCA GCGTGAGAGT
    GAGTTGCTGC GGCTGCAGGA GCGGCTGAAC CAGGAGCGCG AGGAGCTGGA GCGCCAGCGC
    CAGGCCTACC AGCATGACCT GGAGCGGCTG CGCGAGGCCC AGCGCGCCGT GGAGCGGGAG
    CGCGAGCGCC TAGAGGTGCT GCGCCGGCTC AAGAAGCAGA ACACAGCGCC TGGGGCGCTG
    CCGCCGGAGG CACTGGCGGA GACCCAGCCT CTAAGCCACA CTCCCAGCTT CAATGGGAAT
    GGGCTGGATG GATCCATGAC CCTGACCAAA GCACCAGGGC CCCGACCCAC CTCGCTGGTG
    TCCAGTGCGG GGTCCGAGTA TGCCGAGCGC CCCGAGGTGG CCCGCAGGGA CAGCGCCCCG
    GCCAAGAGCG AAGTGCCCAT CCAGCTGCTG AGTGCCACCA ACCAGATCCA GAGGCAGGCA
    GCTGTGCAGC AGCAGATTCC CACCAAGTTG GCAGCCTCCA CCAAGGGCGG AAAGGACAAG
    GGAGGCAAGA ACCGGGGCTC CCAGCGCTGG GACAGCTCGG CCTCGTTTGA CCTGAGGCAG
    CCGCTGCTTC TCAACAAGCT CATGGGCAAA GAAGAGAACA CCTCTCGGAA CCGCCGCTCG
    CTGAGTCCCG TCCTGCCCAG CTCCACGCCC CACCAAGACC CCAGCCTTCC AGCCCCGACC
    CCGGGCCCAG CCGATGTCCC CACCGAGTCC CTCCCCCTCA AGACCTGGGG GCCATCCTTT
    CCACCCGGGG CCCCGTCCTC CACACCAGTC AACAAGGAAG AAACGGGCAA GGAAGACGTC
    ATCTTCTTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850680.1
    CDS491..3622
    Translation

    Target ORF information:

    RefSeq Version XM_024994912.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT TCGAACCTCA 
    CCTGGTTTGA GTTCTTGTCT GACATCCCTG AAGGAGCACC CGAGGGCCAC CCTCCTGTCT
    GATGGCAGCC CACGCCCGAC CAGGAATGTC GGCATGACGG TCTCGCAGAA GGGGGGTCCA
    CAGCCAGCAC CCAGCCCAGC GGGAACCGGG ACAAGACTCG GACCACTCAC AGGAGAGATG
    GATGAAACTG ATACACTGTT TCCGAAGCTC AAGCAGGTGG CCGATGACTC GCTTTTTCTT
    ACATCTTCCA GCACTGAGCC CATCTTTATA GAAGACCCCT ACACTGCCTC GCTGAGAGGC
    GAGGTCGAAG CGGACACCCA CGAGTTCGAG GCTGAGTCCT GGAGCCTCTC TGTGGACCCC
    GAGTATGTGA AGAAACAGAA GAGGGAGGTG GTGAAAAGGC AGGACGTGCT TTATGAGCTG
    ATGCAGACGG AGGCCCACCA TGTGCGGACG CTCAAGATCA TGCTCAAGGT CTACTCCCGG
    GCCCTGCGGG AGGAGCTGCA ATTCAGCCCC AAGGCCGTCA GCCGCCTGTT CCCAGGCGCA
    GACGACCTGC TGGAGGTGCA CAGCCACTTC CTGTGTCGGC TGAAGGAGCG CCGCCTGGAG
    TCGCTGGAGG AGGGCAGTGC CCACAACTAC ATCATCCGGA AGATCGGGGA CCTCCTGCTT
    CAGCAGTTTT CAGGTGAAAA CGGAGAGAGG ATGAAAGAAA AATACAGCGT CTTTTGTAGC
    GGTCACAACG ATGCTGTCAG TCACTACAAG TTGCTGCTGC AGCAAAACAA GAAATTTCAA
    AACTTGATCA AGAGAATCGG CAACTTCTCC ATCGTGAGGC GGCTCGGCGT GCAGGAGTGT
    ATCCTCCTGG TCACCCAACG CATCACCAAA TACCCTGTGC TGGTAGAGCG AATTATCCAG
    AATACAGAAG CTGGCTCCGA GGACCACAGA GACCTGACCC AGGCCCTGAA CCTCATCAAA
    GACATCATCT CACAAGTGGA CGCCAGGGTC AGTGAGTGCG AGAAGGGCCA GCGCCTCAGG
    GAGATCGCTG GGAAGATGGA CCTCAAGTCC TCCAGCAAGC TCAAAAATGG GCTGACCTTC
    CGCAAGGAGG ACATGCTGCA GAGGCAGCTC CACCTGGAGG GCACCCTGTG CTGGAAGACC
    ACCTCTGGGC GCCTGAAAGA CGTCCTCGCA GTCCTGTTGA CAGATGTGCT GCTGCTGCTC
    CAAGAAAAGG ATCAGAAATA CGTCTTTGCA TCCGTGGACT CCAAGCGCCC CGTCATCTCG
    CTGCAAAAGC TCATCGTGAG GGAGGTAGCC AACGAGGAGA AAGCCATGTT TCTGATCAGT
    GCCTCCCTGC ATGGGCCCGA GATGTACGAG ATCTATACCA GCTCGAAGGA AGACAGGAAC
    ACCTGGATGG CCCACATCCG GAGGGCCGTG GAGAGCTGCC CAGACGAAGA GGAAGGGCCC
    TTCAGCGAGG CCGAAGAGGA AAAGAAGGTG GTCGAGACGC GTGCTGTGAG GCTGAGGGAC
    TTCCAAGAGC GACTGAACAG GAAAGACCAG CAGATTGCAC AGAGCCTGAG CGAGAAGCAG
    CAGATCTACC TGGAGATGGC AGAGATGAGC GGGCTCGAGG ACCTCGCCCT GTCTCGGCTT
    CTCTTCCGGG GAGGGGACCC CCCCGAGAAC CTGCAGGGCG AGCTGATTCT CAAGTCAGCC
    ATGAGTGAGA TCGAAGACAT CCAGAGTTTC ATCTGCAGGC GGCTGGGCAA CGCCTACTGC
    CAGGCAGAAG ACGGGGGCAG CGCAGGCAGC CCAGGCAAGG ACGGCAGTCT TCAGAAGAAC
    ATGTGCAGCA GTGACCTCAG CCCCCGAGAC TGGCAGGGCC TGGTGAGCAG CCTGGACTCG
    AGGCCTGGAG ACACCCAGGG GGCCCCCGAG GAAGCCTCAA AGGTGGCAGA GTCACCAGGT
    GCAGACCCCG GCCCCCACCC ACCTGCGGTC CTGGAGTTGG AGCTCGTCCA GCGGATCCAG
    ACGCTGTCCC AGCTGCTCCT CAGCCTCCAG GTGGTCATCG CCCAGCAGGA CAGCTACGTG
    GAAATTCAGA AAGCAGCCAT CCAGGAGCGC GAGAAGCAGT TCCGGCTGCA GTCAACACGC
    GGGAACCTGC TGCTGGAACA GGAGCGGCAG CGCAACTTTG AGAAGCAGCG AGAGGAGCTG
    GCAGGCGTGC AGAAGCTGCA GGGCCAGCTG CGGCTGGAGC AGCAACGCTG GGAGCGCGAG
    CGGGAGCAGC AGCGCCGGGA GCTGGACCAG GCCTCGGCGC GGCTGCAGCA GCGTGAGAGT
    GAGTTGCTGC GGCTGCAGGA GCGGCTGAAC CAGGAGCGCG AGGAGCTGGA GCGCCAGCGC
    CAGGCCTACC AGCATGACCT GGAGCGGCTG CGCGAGGCCC AGCGCGCCGT GGAGCGGGAG
    CGCGAGCGCC TAGAGGTGCT GCGCCGGCTC AAGAAGCAGA ACACAGCGCC TGGGGCGCTG
    CCGCCGGAGG CACTGGCGGA GACCCAGCCT CTAAGCCACA CTCCCAGCTT CAATGGGAAT
    GGGCTGGATG GATCCATGAC CCTGACCAAA GCACCAGGGC CCCGACCCAC CTCGCTGGTG
    TCCAGTGCGG GGTCCGAGTA TGCCGAGCGC CCCGAGGTGG CCCGCAGGGA CAGCGCCCCG
    GCCAAGAGCG AAGTGCCCAT CCAGCTGCTG AGTGCCACCA ACCAGATCCA GAGGCAGGCA
    GCTGTGCAGC AGCAGATTCC CACCAAGTTG GCAGCCTCCA CCAAGGGCGG AAAGGACAAG
    GGAGGCAAGA ACCGGGGCTC CCAGCGCTGG GACAGCTCGG CCTCGTTTGA CCTGAGGCAG
    CCGCTGCTTC TCAACAAGCT CATGGGCAAA GAAGAGAACA CCTCTCGGAA CCGCCGCTCG
    CTGAGTCCCG TCCTGCCCAG CTCCACGCCC CACCAAGACC CCAGCCTTCC AGCCCCGACC
    CCGGGCCCAG CCGATGTCCC CACCGAGTCC CTCCCCCTCA AGACCTGGGG GCCATCCTTT
    CCACCCGGGG CCCCGTCCTC CACACCAGTC AACAAGGAAG AAACGGGCAA GGAAGACGTC
    ATCTTCTTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90243
    Clone ID Related Accession (Same CDS sequence) XM_015464087.1 , XM_015472030.1
    Accession Version XM_015464087.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGTCTGG CAGCCCCTGC AGACAGTCTC CGCCCCTCTC GCTGGCAGCC CTGTCCTGGC 
    AGGGCTTCCC AGCCGAGCGC CTTCTTCATC CTGCCGTTCA CTCACACGCT CGCACGCACG
    GCCCCCATGG CCAGCTCGGC GCTCAACCAC TCCTGGCCGG CTCTCTCCAA GTTCTGGCTG
    AAGCGGTGGG CCTTTAAGAG AGGCTCTGAG CCCAAGCCAT GTGTCCAGCC CTTTGACTCT
    GGAGCTGCAG ATTCAGCCAC CAAGAGCAGC GGTGGACCCT GGAGGCCTGA GGACCCAGAC
    TTCTCCACTA TGGAGGATGA GCAGGAGGAT GGAATCCCAG GGCTGCTCGG CGGTAGCGAG
    AGTTCAGAGG ACCTCAGCCT GGATTTGGGG GCCCTTCAGG GCAGTGAGTA TCTCCAGGAC
    CTGGGCCTCG AAGTCCTTTC ACACAGCCAG CCTGGGGGAG CCAGAGACAG TGGCCCCCCT
    AGTGAAGAAG CCCGAGGAGA AACGCCCCGA GGAGAAACGC CCCTCTCCAG CGCTGCGAGG
    TCCCAGGGCC TTGCTCGGAG ACGCAGCTGG GAGAGGTCAA GGAGCTGCTC TGAGAGCCGG
    CAGAGGCTCA GCATCGAATC CTCAGCTGTG AATGAGGGGC CCTTTCTGCC TCGGACACTG
    GCCAGCCTCG CTCTGAACCT GCCAGGAGAG GGCCTGCAGG CCTGGACCCA AGGGTGTCTG
    CCTGGGGGTG GAACCCCCGC AGAGCAACCA AGCAAGGAAT GTGACAGCCC CGAGAAAAGA
    GTGAGGTCAC GGTCTGTTCC TGTGTCCTTC GATGAGATCA GCTCCCTGGA AATCTTTCCA
    GCTTTGGAAG TGCCACCTCC AGCTGTTCAA GGCCTGGACC CCCCGGTGCT GGAGTGCATG
    GAAAAGGATC ATGTGGAGCC GAATCATGTG TTGATTGTCC AGCAGGTGCT TGAAGAACTC
    CGACAGTACC ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT
    TCGAACCTCA CCTGGTTTGA GTTCTTGTCT GAGAGTGAGG ACAGCATGGG GAAGACGGAG
    AGAAGCGACA GGGGCACCAA GGTGAAACGC AGCCTGAGCT CCCTGCGCAA TCGAGTCACC
    AGGCAGAAGG AGAAGGGGAA GACCCCAGTG CATCAGAAGG ACAAAGGTCA GGATGCGCGA
    GAGAGGAAAG AATGTATCAA CGGGCACCAG CTGGCTCGAG GAACCTTCTC TGGCCACTCC
    AGCTGCCCTA TGTGCGGCAA ACCTTTTCTG AGCTCTGCAT CCCTGAAGGA GCACCCGAGG
    GCCACCCTCC TGTCTGATGG CAGCCCACGC CCGACCAGGA ATGTCGGCAT GACGGTCTCG
    CAGAAGGGGG GTCCACAGCC AGCACCCAGC CCAGCGGGAA CCGGGACAAG ACTCGGACCA
    CTCACAGGAG AGATGGATGA AACTGATACA CTGTTTCCGA AGCTCAAGCA GGTGGCCGAT
    GACTCGCTTT TTCTTACATC TTCCAGCACT GAGCCCATCT TTATAGAAGA CCCCTACACT
    GCCTCGCTGA GAGGCGAGGT CGAAGCGGAC ACCCACGAGT TCGAGGCTGA GTCCTGGAGC
    CTCTCTGTGG ACCCCGAGTA TGTGAAGAAA CAGAAGAGGG AGGTGGTGAA AAGGCAGGAC
    GTGCTTTATG AGCTGATGCA GACGGAGGCC CACCATGTGC GGACGCTCAA GATCATGCTC
    AAGGTCTACT CCCGGGCCCT GCGGGAGGAG CTGCAATTCA GCCCCAAGGC CGTCAGCCGC
    CTGTTCCCAG GCGCAGACGA CCTGCTGGAG GTGCACAGCC ACTTCCTGTG TCGGCTGAAG
    GAGCGCCGCC TGGAGTCGCT GGAGGAGGGC AGTGCCCACA ACTACATCAT CCGGAAGATC
    GGGGACCTCC TGCTTCAGCA GTTTTCAGGT GAAAACGGAG AGAGGATGAA AGAAAAATAC
    AGCGTCTTTT GTAGCGGTCA CAACGATGCT GTCAGTCACT ACAAGTTGCT GCTGCAGCAA
    AACAAGAAAT TTCAAAACTT GATCAAGAGA ATCGGCAACT TCTCCATCGT GAGGCGGCTC
    GGCGTGCAGG AGTGTATCCT CCTGGTCACC CAACGCATCA CCAAATACCC TGTGCTGGTA
    GAGCGAATTA TCCAGAATAC AGAAGCTGGC TCCGAGGACC ACAGAGACCT GACCCAGGCC
    CTGAACCTCA TCAAAGACAT CATCTCACAA GTGGACGCCA GGGTCAGTGA GTGCGAGAAG
    GGCCAGCGCC TCAGGGAGAT CGCTGGGAAG ATGGACCTCA AGTCCTCCAG CAAGCTCAAA
    AATGGGCTGA CCTTCCGCAA GGAGGACATG CTGCAGAGGC AGCTCCACCT GGAGGGCACC
    CTGTGCTGGA AGACCACCTC TGGGCGCCTG AAAGACGTCC TCGCAGTCCT GTTGACAGAT
    GTGCTGCTGC TGCTCCAAGA AAAGGATCAG AAATACGTCT TTGCATCCGT GGACTCCAAG
    CGCCCCGTCA TCTCGCTGCA AAAGCTCATC GTGAGGGAGG TAGCCAACGA GGAGAAAGCC
    ATGTTTCTGA TCAGTGCCTC CCTGCATGGG CCCGAGATGT ACGAGATCTA TACCAGCTCG
    AAGGAAGACA GGAACACCTG GATGGCCCAC ATCCGGAGGG CCGTGGAGAG CTGCCCAGAC
    GAAGAGGAAG GGCCCTTCAG CGAGGCCGAA GAGGAAAAGA AGGTGGTCGA GACGCGTGCT
    GTGAGGCTGA GGGACTTCCA AGAGCGACTG AACAGGAAAG ACCAGCAGAT TGCACAGAGC
    CTGAGCGAGA AGCAGCAGAT CTACCTGGAG ATGGCAGAGA TGAGCGGGCT CGAGGACCTC
    GCCCTGTCTC GGCTTCTCTT CCGGGGAGGG GACCCCCCCG AGAACCTGCA GGGCGAGCTG
    ATTCTCAAGT CAGCCATGAG TGAGATCGAA GACATCCAGA GTTTCATCTG CAGGCGGCTG
    GGCAACGCCT ACTGCCAGGC AGAAGACGGG GGCAGCGCAG GCAGCCCAGG CAAGGACGGC
    AGTCTTCAGA AGAACATGTG CAGCAGTGAC CTCAGCCCCC GAGACTGGCA GGGCCTGGTG
    AGCAGCCTGG ACTCGAGGCC TGGAGACACC CAGGGGGCCC CCGAGGAAGC CTCAAAGGTG
    GCAGAGTCAC CAGGTGCAGA CCCCGGCCCC CACCCACCTG CGGTCCTGGA GTTGGAGCTC
    GTCCAGCGGA TCCAGACGCT GTCCCAGCTG CTCCTCAGCC TCCAGGTGGT CATCGCCCAG
    CAGGACAGCT ACGTGGAAAT TCAGAAAGCA GCCATCCAGG AGCGCGAGAA GCAGTTCCGG
    CTGCAGTCAA CACGCGGGAA CCTGCTGCTG GAACAGGAGC GGCAGCGCAA CTTTGAGAAG
    CAGCGAGAGG AGCTGGCAGG CGTGCAGAAG CTGCAGGGCC AGCTGCGGCT GGAGCAGCAA
    CGCTGGGAGC GCGAGCGGGA GCAGCAGCGC CGGGAGCTGG ACCAGGCCTC GGCGCGGCTG
    CAGCAGCGTG AGAGTGAGTT GCTGCGGCTG CAGGAGCGGC TGAACCAGGA GCGCGAGGAG
    CTGGAGCGCC AGCGCCAGGC CTACCAGCAT GACCTGGAGC GGCTGCGCGA GGCCCAGCGC
    GCCGTGGAGC GGGAGCGCGA GCGCCTAGAG GTGCTGCGCC GGCTCAAGAA GCAGAACACA
    GCGCCTGGGG CGCTGCCGCC GGAGGCACTG GCGGAGACCC AGCCTCTAAG CCACACTCCC
    AGCTTCAATG GGAATGGGCT GGATGGATCC ATGACCCTGA CCAAAGCACC AGGGCCCCGA
    CCCACCTCGC TGGTGTCCAG TGCGGGGTCC GAGTATGCCG AGCGCCCCGA GGTGGCCCGC
    AGGGACAGCG CCCCGGCCAA GAGCGAAGTG CCCATCCAGC TGCTGAGTGC CACCAACCAG
    ATCCAGAGGC AGGCAGCTGT GCAGCAGCAG ATTCCCACCA AGTTGGCAGC CTCCACCAAG
    GGCGGAAAGG ACAAGGGAGG CAAGAACCGG GGCTCCCAGC GCTGGGACAG CTCGGCCTCG
    TTTGACCTGA GGCAGCCGCT GCTTCTCAAC AAGCTCATGG GCAAAGAAGA GAACACCTCT
    CGGAACCGCC GCTCGCTGAG TCCCGTCCTG CCCAGCTCCA CGCCCCACCA AGACCCCAGC
    CTTCCAGCCC CGACCCCGGG CCCAGCCGAT GTCCCCACCG AGTCCCTCCC CCTCAAGACC
    TGGGGGCCAT CCTTTCCACC CGGGGCCCCG TCCTCCACAC CAGTCAACAA GGAAGAAACG
    GGCAAGGAAG ACGTCATCTT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319573.1
    CDS335..4681
    Translation

    Target ORF information:

    RefSeq Version XM_015464087.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464087.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGTCTGG CAGCCCCTGC AGACAGTCTC CGCCCCTCTC GCTGGCAGCC CTGTCCTGGC 
    AGGGCTTCCC AGCCGAGCGC CTTCTTCATC CTGCCGTTCA CTCACACGCT CGCACGCACG
    GCCCCCATGG CCAGCTCGGC GCTCAACCAC TCCTGGCCGG CTCTCTCCAA GTTCTGGCTG
    AAGCGGTGGG CCTTTAAGAG AGGCTCTGAG CCCAAGCCAT GTGTCCAGCC CTTTGACTCT
    GGAGCTGCAG ATTCAGCCAC CAAGAGCAGC GGTGGACCCT GGAGGCCTGA GGACCCAGAC
    TTCTCCACTA TGGAGGATGA GCAGGAGGAT GGAATCCCAG GGCTGCTCGG CGGTAGCGAG
    AGTTCAGAGG ACCTCAGCCT GGATTTGGGG GCCCTTCAGG GCAGTGAGTA TCTCCAGGAC
    CTGGGCCTCG AAGTCCTTTC ACACAGCCAG CCTGGGGGAG CCAGAGACAG TGGCCCCCCT
    AGTGAAGAAG CCCGAGGAGA AACGCCCCGA GGAGAAACGC CCCTCTCCAG CGCTGCGAGG
    TCCCAGGGCC TTGCTCGGAG ACGCAGCTGG GAGAGGTCAA GGAGCTGCTC TGAGAGCCGG
    CAGAGGCTCA GCATCGAATC CTCAGCTGTG AATGAGGGGC CCTTTCTGCC TCGGACACTG
    GCCAGCCTCG CTCTGAACCT GCCAGGAGAG GGCCTGCAGG CCTGGACCCA AGGGTGTCTG
    CCTGGGGGTG GAACCCCCGC AGAGCAACCA AGCAAGGAAT GTGACAGCCC CGAGAAAAGA
    GTGAGGTCAC GGTCTGTTCC TGTGTCCTTC GATGAGATCA GCTCCCTGGA AATCTTTCCA
    GCTTTGGAAG TGCCACCTCC AGCTGTTCAA GGCCTGGACC CCCCGGTGCT GGAGTGCATG
    GAAAAGGATC ATGTGGAGCC GAATCATGTG TTGATTGTCC AGCAGGTGCT TGAAGAACTC
    CGACAGTACC ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT
    TCGAACCTCA CCTGGTTTGA GTTCTTGTCT GAGAGTGAGG ACAGCATGGG GAAGACGGAG
    AGAAGCGACA GGGGCACCAA GGTGAAACGC AGCCTGAGCT CCCTGCGCAA TCGAGTCACC
    AGGCAGAAGG AGAAGGGGAA GACCCCAGTG CATCAGAAGG ACAAAGGTCA GGATGCGCGA
    GAGAGGAAAG AATGTATCAA CGGGCACCAG CTGGCTCGAG GAACCTTCTC TGGCCACTCC
    AGCTGCCCTA TGTGCGGCAA ACCTTTTCTG AGCTCTGCAT CCCTGAAGGA GCACCCGAGG
    GCCACCCTCC TGTCTGATGG CAGCCCACGC CCGACCAGGA ATGTCGGCAT GACGGTCTCG
    CAGAAGGGGG GTCCACAGCC AGCACCCAGC CCAGCGGGAA CCGGGACAAG ACTCGGACCA
    CTCACAGGAG AGATGGATGA AACTGATACA CTGTTTCCGA AGCTCAAGCA GGTGGCCGAT
    GACTCGCTTT TTCTTACATC TTCCAGCACT GAGCCCATCT TTATAGAAGA CCCCTACACT
    GCCTCGCTGA GAGGCGAGGT CGAAGCGGAC ACCCACGAGT TCGAGGCTGA GTCCTGGAGC
    CTCTCTGTGG ACCCCGAGTA TGTGAAGAAA CAGAAGAGGG AGGTGGTGAA AAGGCAGGAC
    GTGCTTTATG AGCTGATGCA GACGGAGGCC CACCATGTGC GGACGCTCAA GATCATGCTC
    AAGGTCTACT CCCGGGCCCT GCGGGAGGAG CTGCAATTCA GCCCCAAGGC CGTCAGCCGC
    CTGTTCCCAG GCGCAGACGA CCTGCTGGAG GTGCACAGCC ACTTCCTGTG TCGGCTGAAG
    GAGCGCCGCC TGGAGTCGCT GGAGGAGGGC AGTGCCCACA ACTACATCAT CCGGAAGATC
    GGGGACCTCC TGCTTCAGCA GTTTTCAGGT GAAAACGGAG AGAGGATGAA AGAAAAATAC
    AGCGTCTTTT GTAGCGGTCA CAACGATGCT GTCAGTCACT ACAAGTTGCT GCTGCAGCAA
    AACAAGAAAT TTCAAAACTT GATCAAGAGA ATCGGCAACT TCTCCATCGT GAGGCGGCTC
    GGCGTGCAGG AGTGTATCCT CCTGGTCACC CAACGCATCA CCAAATACCC TGTGCTGGTA
    GAGCGAATTA TCCAGAATAC AGAAGCTGGC TCCGAGGACC ACAGAGACCT GACCCAGGCC
    CTGAACCTCA TCAAAGACAT CATCTCACAA GTGGACGCCA GGGTCAGTGA GTGCGAGAAG
    GGCCAGCGCC TCAGGGAGAT CGCTGGGAAG ATGGACCTCA AGTCCTCCAG CAAGCTCAAA
    AATGGGCTGA CCTTCCGCAA GGAGGACATG CTGCAGAGGC AGCTCCACCT GGAGGGCACC
    CTGTGCTGGA AGACCACCTC TGGGCGCCTG AAAGACGTCC TCGCAGTCCT GTTGACAGAT
    GTGCTGCTGC TGCTCCAAGA AAAGGATCAG AAATACGTCT TTGCATCCGT GGACTCCAAG
    CGCCCCGTCA TCTCGCTGCA AAAGCTCATC GTGAGGGAGG TAGCCAACGA GGAGAAAGCC
    ATGTTTCTGA TCAGTGCCTC CCTGCATGGG CCCGAGATGT ACGAGATCTA TACCAGCTCG
    AAGGAAGACA GGAACACCTG GATGGCCCAC ATCCGGAGGG CCGTGGAGAG CTGCCCAGAC
    GAAGAGGAAG GGCCCTTCAG CGAGGCCGAA GAGGAAAAGA AGGTGGTCGA GACGCGTGCT
    GTGAGGCTGA GGGACTTCCA AGAGCGACTG AACAGGAAAG ACCAGCAGAT TGCACAGAGC
    CTGAGCGAGA AGCAGCAGAT CTACCTGGAG ATGGCAGAGA TGAGCGGGCT CGAGGACCTC
    GCCCTGTCTC GGCTTCTCTT CCGGGGAGGG GACCCCCCCG AGAACCTGCA GGGCGAGCTG
    ATTCTCAAGT CAGCCATGAG TGAGATCGAA GACATCCAGA GTTTCATCTG CAGGCGGCTG
    GGCAACGCCT ACTGCCAGGC AGAAGACGGG GGCAGCGCAG GCAGCCCAGG CAAGGACGGC
    AGTCTTCAGA AGAACATGTG CAGCAGTGAC CTCAGCCCCC GAGACTGGCA GGGCCTGGTG
    AGCAGCCTGG ACTCGAGGCC TGGAGACACC CAGGGGGCCC CCGAGGAAGC CTCAAAGGTG
    GCAGAGTCAC CAGGTGCAGA CCCCGGCCCC CACCCACCTG CGGTCCTGGA GTTGGAGCTC
    GTCCAGCGGA TCCAGACGCT GTCCCAGCTG CTCCTCAGCC TCCAGGTGGT CATCGCCCAG
    CAGGACAGCT ACGTGGAAAT TCAGAAAGCA GCCATCCAGG AGCGCGAGAA GCAGTTCCGG
    CTGCAGTCAA CACGCGGGAA CCTGCTGCTG GAACAGGAGC GGCAGCGCAA CTTTGAGAAG
    CAGCGAGAGG AGCTGGCAGG CGTGCAGAAG CTGCAGGGCC AGCTGCGGCT GGAGCAGCAA
    CGCTGGGAGC GCGAGCGGGA GCAGCAGCGC CGGGAGCTGG ACCAGGCCTC GGCGCGGCTG
    CAGCAGCGTG AGAGTGAGTT GCTGCGGCTG CAGGAGCGGC TGAACCAGGA GCGCGAGGAG
    CTGGAGCGCC AGCGCCAGGC CTACCAGCAT GACCTGGAGC GGCTGCGCGA GGCCCAGCGC
    GCCGTGGAGC GGGAGCGCGA GCGCCTAGAG GTGCTGCGCC GGCTCAAGAA GCAGAACACA
    GCGCCTGGGG CGCTGCCGCC GGAGGCACTG GCGGAGACCC AGCCTCTAAG CCACACTCCC
    AGCTTCAATG GGAATGGGCT GGATGGATCC ATGACCCTGA CCAAAGCACC AGGGCCCCGA
    CCCACCTCGC TGGTGTCCAG TGCGGGGTCC GAGTATGCCG AGCGCCCCGA GGTGGCCCGC
    AGGGACAGCG CCCCGGCCAA GAGCGAAGTG CCCATCCAGC TGCTGAGTGC CACCAACCAG
    ATCCAGAGGC AGGCAGCTGT GCAGCAGCAG ATTCCCACCA AGTTGGCAGC CTCCACCAAG
    GGCGGAAAGG ACAAGGGAGG CAAGAACCGG GGCTCCCAGC GCTGGGACAG CTCGGCCTCG
    TTTGACCTGA GGCAGCCGCT GCTTCTCAAC AAGCTCATGG GCAAAGAAGA GAACACCTCT
    CGGAACCGCC GCTCGCTGAG TCCCGTCCTG CCCAGCTCCA CGCCCCACCA AGACCCCAGC
    CTTCCAGCCC CGACCCCGGG CCCAGCCGAT GTCCCCACCG AGTCCCTCCC CCTCAAGACC
    TGGGGGCCAT CCTTTCCACC CGGGGCCCCG TCCTCCACAC CAGTCAACAA GGAAGAAACG
    GGCAAGGAAG ACGTCATCTT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90243
    Clone ID Related Accession (Same CDS sequence) XM_015464087.1 , XM_015472030.1
    Accession Version XM_015472030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGTCTGG CAGCCCCTGC AGACAGTCTC CGCCCCTCTC GCTGGCAGCC CTGTCCTGGC 
    AGGGCTTCCC AGCCGAGCGC CTTCTTCATC CTGCCGTTCA CTCACACGCT CGCACGCACG
    GCCCCCATGG CCAGCTCGGC GCTCAACCAC TCCTGGCCGG CTCTCTCCAA GTTCTGGCTG
    AAGCGGTGGG CCTTTAAGAG AGGCTCTGAG CCCAAGCCAT GTGTCCAGCC CTTTGACTCT
    GGAGCTGCAG ATTCAGCCAC CAAGAGCAGC GGTGGACCCT GGAGGCCTGA GGACCCAGAC
    TTCTCCACTA TGGAGGATGA GCAGGAGGAT GGAATCCCAG GGCTGCTCGG CGGTAGCGAG
    AGTTCAGAGG ACCTCAGCCT GGATTTGGGG GCCCTTCAGG GCAGTGAGTA TCTCCAGGAC
    CTGGGCCTCG AAGTCCTTTC ACACAGCCAG CCTGGGGGAG CCAGAGACAG TGGCCCCCCT
    AGTGAAGAAG CCCGAGGAGA AACGCCCCGA GGAGAAACGC CCCTCTCCAG CGCTGCGAGG
    TCCCAGGGCC TTGCTCGGAG ACGCAGCTGG GAGAGGTCAA GGAGCTGCTC TGAGAGCCGG
    CAGAGGCTCA GCATCGAATC CTCAGCTGTG AATGAGGGGC CCTTTCTGCC TCGGACACTG
    GCCAGCCTCG CTCTGAACCT GCCAGGAGAG GGCCTGCAGG CCTGGACCCA AGGGTGTCTG
    CCTGGGGGTG GAACCCCCGC AGAGCAACCA AGCAAGGAAT GTGACAGCCC CGAGAAAAGA
    GTGAGGTCAC GGTCTGTTCC TGTGTCCTTC GATGAGATCA GCTCCCTGGA AATCTTTCCA
    GCTTTGGAAG TGCCACCTCC AGCTGTTCAA GGCCTGGACC CCCCGGTGCT GGAGTGCATG
    GAAAAGGATC ATGTGGAGCC GAATCATGTG TTGATTGTCC AGCAGGTGCT TGAAGAACTC
    CGACAGTACC ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT
    TCGAACCTCA CCTGGTTTGA GTTCTTGTCT GAGAGTGAGG ACAGCATGGG GAAGACGGAG
    AGAAGCGACA GGGGCACCAA GGTGAAACGC AGCCTGAGCT CCCTGCGCAA TCGAGTCACC
    AGGCAGAAGG AGAAGGGGAA GACCCCAGTG CATCAGAAGG ACAAAGGTCA GGATGCGCGA
    GAGAGGAAAG AATGTATCAA CGGGCACCAG CTGGCTCGAG GAACCTTCTC TGGCCACTCC
    AGCTGCCCTA TGTGCGGCAA ACCTTTTCTG AGCTCTGCAT CCCTGAAGGA GCACCCGAGG
    GCCACCCTCC TGTCTGATGG CAGCCCACGC CCGACCAGGA ATGTCGGCAT GACGGTCTCG
    CAGAAGGGGG GTCCACAGCC AGCACCCAGC CCAGCGGGAA CCGGGACAAG ACTCGGACCA
    CTCACAGGAG AGATGGATGA AACTGATACA CTGTTTCCGA AGCTCAAGCA GGTGGCCGAT
    GACTCGCTTT TTCTTACATC TTCCAGCACT GAGCCCATCT TTATAGAAGA CCCCTACACT
    GCCTCGCTGA GAGGCGAGGT CGAAGCGGAC ACCCACGAGT TCGAGGCTGA GTCCTGGAGC
    CTCTCTGTGG ACCCCGAGTA TGTGAAGAAA CAGAAGAGGG AGGTGGTGAA AAGGCAGGAC
    GTGCTTTATG AGCTGATGCA GACGGAGGCC CACCATGTGC GGACGCTCAA GATCATGCTC
    AAGGTCTACT CCCGGGCCCT GCGGGAGGAG CTGCAATTCA GCCCCAAGGC CGTCAGCCGC
    CTGTTCCCAG GCGCAGACGA CCTGCTGGAG GTGCACAGCC ACTTCCTGTG TCGGCTGAAG
    GAGCGCCGCC TGGAGTCGCT GGAGGAGGGC AGTGCCCACA ACTACATCAT CCGGAAGATC
    GGGGACCTCC TGCTTCAGCA GTTTTCAGGT GAAAACGGAG AGAGGATGAA AGAAAAATAC
    AGCGTCTTTT GTAGCGGTCA CAACGATGCT GTCAGTCACT ACAAGTTGCT GCTGCAGCAA
    AACAAGAAAT TTCAAAACTT GATCAAGAGA ATCGGCAACT TCTCCATCGT GAGGCGGCTC
    GGCGTGCAGG AGTGTATCCT CCTGGTCACC CAACGCATCA CCAAATACCC TGTGCTGGTA
    GAGCGAATTA TCCAGAATAC AGAAGCTGGC TCCGAGGACC ACAGAGACCT GACCCAGGCC
    CTGAACCTCA TCAAAGACAT CATCTCACAA GTGGACGCCA GGGTCAGTGA GTGCGAGAAG
    GGCCAGCGCC TCAGGGAGAT CGCTGGGAAG ATGGACCTCA AGTCCTCCAG CAAGCTCAAA
    AATGGGCTGA CCTTCCGCAA GGAGGACATG CTGCAGAGGC AGCTCCACCT GGAGGGCACC
    CTGTGCTGGA AGACCACCTC TGGGCGCCTG AAAGACGTCC TCGCAGTCCT GTTGACAGAT
    GTGCTGCTGC TGCTCCAAGA AAAGGATCAG AAATACGTCT TTGCATCCGT GGACTCCAAG
    CGCCCCGTCA TCTCGCTGCA AAAGCTCATC GTGAGGGAGG TAGCCAACGA GGAGAAAGCC
    ATGTTTCTGA TCAGTGCCTC CCTGCATGGG CCCGAGATGT ACGAGATCTA TACCAGCTCG
    AAGGAAGACA GGAACACCTG GATGGCCCAC ATCCGGAGGG CCGTGGAGAG CTGCCCAGAC
    GAAGAGGAAG GGCCCTTCAG CGAGGCCGAA GAGGAAAAGA AGGTGGTCGA GACGCGTGCT
    GTGAGGCTGA GGGACTTCCA AGAGCGACTG AACAGGAAAG ACCAGCAGAT TGCACAGAGC
    CTGAGCGAGA AGCAGCAGAT CTACCTGGAG ATGGCAGAGA TGAGCGGGCT CGAGGACCTC
    GCCCTGTCTC GGCTTCTCTT CCGGGGAGGG GACCCCCCCG AGAACCTGCA GGGCGAGCTG
    ATTCTCAAGT CAGCCATGAG TGAGATCGAA GACATCCAGA GTTTCATCTG CAGGCGGCTG
    GGCAACGCCT ACTGCCAGGC AGAAGACGGG GGCAGCGCAG GCAGCCCAGG CAAGGACGGC
    AGTCTTCAGA AGAACATGTG CAGCAGTGAC CTCAGCCCCC GAGACTGGCA GGGCCTGGTG
    AGCAGCCTGG ACTCGAGGCC TGGAGACACC CAGGGGGCCC CCGAGGAAGC CTCAAAGGTG
    GCAGAGTCAC CAGGTGCAGA CCCCGGCCCC CACCCACCTG CGGTCCTGGA GTTGGAGCTC
    GTCCAGCGGA TCCAGACGCT GTCCCAGCTG CTCCTCAGCC TCCAGGTGGT CATCGCCCAG
    CAGGACAGCT ACGTGGAAAT TCAGAAAGCA GCCATCCAGG AGCGCGAGAA GCAGTTCCGG
    CTGCAGTCAA CACGCGGGAA CCTGCTGCTG GAACAGGAGC GGCAGCGCAA CTTTGAGAAG
    CAGCGAGAGG AGCTGGCAGG CGTGCAGAAG CTGCAGGGCC AGCTGCGGCT GGAGCAGCAA
    CGCTGGGAGC GCGAGCGGGA GCAGCAGCGC CGGGAGCTGG ACCAGGCCTC GGCGCGGCTG
    CAGCAGCGTG AGAGTGAGTT GCTGCGGCTG CAGGAGCGGC TGAACCAGGA GCGCGAGGAG
    CTGGAGCGCC AGCGCCAGGC CTACCAGCAT GACCTGGAGC GGCTGCGCGA GGCCCAGCGC
    GCCGTGGAGC GGGAGCGCGA GCGCCTAGAG GTGCTGCGCC GGCTCAAGAA GCAGAACACA
    GCGCCTGGGG CGCTGCCGCC GGAGGCACTG GCGGAGACCC AGCCTCTAAG CCACACTCCC
    AGCTTCAATG GGAATGGGCT GGATGGATCC ATGACCCTGA CCAAAGCACC AGGGCCCCGA
    CCCACCTCGC TGGTGTCCAG TGCGGGGTCC GAGTATGCCG AGCGCCCCGA GGTGGCCCGC
    AGGGACAGCG CCCCGGCCAA GAGCGAAGTG CCCATCCAGC TGCTGAGTGC CACCAACCAG
    ATCCAGAGGC AGGCAGCTGT GCAGCAGCAG ATTCCCACCA AGTTGGCAGC CTCCACCAAG
    GGCGGAAAGG ACAAGGGAGG CAAGAACCGG GGCTCCCAGC GCTGGGACAG CTCGGCCTCG
    TTTGACCTGA GGCAGCCGCT GCTTCTCAAC AAGCTCATGG GCAAAGAAGA GAACACCTCT
    CGGAACCGCC GCTCGCTGAG TCCCGTCCTG CCCAGCTCCA CGCCCCACCA AGACCCCAGC
    CTTCCAGCCC CGACCCCGGG CCCAGCCGAT GTCCCCACCG AGTCCCTCCC CCTCAAGACC
    TGGGGGCCAT CCTTTCCACC CGGGGCCCCG TCCTCCACAC CAGTCAACAA GGAAGAAACG
    GGCAAGGAAG ACGTCATCTT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327516.1
    CDS335..4681
    Translation

    Target ORF information:

    RefSeq Version XM_015472030.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGTCTGG CAGCCCCTGC AGACAGTCTC CGCCCCTCTC GCTGGCAGCC CTGTCCTGGC 
    AGGGCTTCCC AGCCGAGCGC CTTCTTCATC CTGCCGTTCA CTCACACGCT CGCACGCACG
    GCCCCCATGG CCAGCTCGGC GCTCAACCAC TCCTGGCCGG CTCTCTCCAA GTTCTGGCTG
    AAGCGGTGGG CCTTTAAGAG AGGCTCTGAG CCCAAGCCAT GTGTCCAGCC CTTTGACTCT
    GGAGCTGCAG ATTCAGCCAC CAAGAGCAGC GGTGGACCCT GGAGGCCTGA GGACCCAGAC
    TTCTCCACTA TGGAGGATGA GCAGGAGGAT GGAATCCCAG GGCTGCTCGG CGGTAGCGAG
    AGTTCAGAGG ACCTCAGCCT GGATTTGGGG GCCCTTCAGG GCAGTGAGTA TCTCCAGGAC
    CTGGGCCTCG AAGTCCTTTC ACACAGCCAG CCTGGGGGAG CCAGAGACAG TGGCCCCCCT
    AGTGAAGAAG CCCGAGGAGA AACGCCCCGA GGAGAAACGC CCCTCTCCAG CGCTGCGAGG
    TCCCAGGGCC TTGCTCGGAG ACGCAGCTGG GAGAGGTCAA GGAGCTGCTC TGAGAGCCGG
    CAGAGGCTCA GCATCGAATC CTCAGCTGTG AATGAGGGGC CCTTTCTGCC TCGGACACTG
    GCCAGCCTCG CTCTGAACCT GCCAGGAGAG GGCCTGCAGG CCTGGACCCA AGGGTGTCTG
    CCTGGGGGTG GAACCCCCGC AGAGCAACCA AGCAAGGAAT GTGACAGCCC CGAGAAAAGA
    GTGAGGTCAC GGTCTGTTCC TGTGTCCTTC GATGAGATCA GCTCCCTGGA AATCTTTCCA
    GCTTTGGAAG TGCCACCTCC AGCTGTTCAA GGCCTGGACC CCCCGGTGCT GGAGTGCATG
    GAAAAGGATC ATGTGGAGCC GAATCATGTG TTGATTGTCC AGCAGGTGCT TGAAGAACTC
    CGACAGTACC ATGGGGCCCG GCAGAGGGCT CGATTGTCGG TCTGCCCCGG AGGAGCAGCT
    TCGAACCTCA CCTGGTTTGA GTTCTTGTCT GAGAGTGAGG ACAGCATGGG GAAGACGGAG
    AGAAGCGACA GGGGCACCAA GGTGAAACGC AGCCTGAGCT CCCTGCGCAA TCGAGTCACC
    AGGCAGAAGG AGAAGGGGAA GACCCCAGTG CATCAGAAGG ACAAAGGTCA GGATGCGCGA
    GAGAGGAAAG AATGTATCAA CGGGCACCAG CTGGCTCGAG GAACCTTCTC TGGCCACTCC
    AGCTGCCCTA TGTGCGGCAA ACCTTTTCTG AGCTCTGCAT CCCTGAAGGA GCACCCGAGG
    GCCACCCTCC TGTCTGATGG CAGCCCACGC CCGACCAGGA ATGTCGGCAT GACGGTCTCG
    CAGAAGGGGG GTCCACAGCC AGCACCCAGC CCAGCGGGAA CCGGGACAAG ACTCGGACCA
    CTCACAGGAG AGATGGATGA AACTGATACA CTGTTTCCGA AGCTCAAGCA GGTGGCCGAT
    GACTCGCTTT TTCTTACATC TTCCAGCACT GAGCCCATCT TTATAGAAGA CCCCTACACT
    GCCTCGCTGA GAGGCGAGGT CGAAGCGGAC ACCCACGAGT TCGAGGCTGA GTCCTGGAGC
    CTCTCTGTGG ACCCCGAGTA TGTGAAGAAA CAGAAGAGGG AGGTGGTGAA AAGGCAGGAC
    GTGCTTTATG AGCTGATGCA GACGGAGGCC CACCATGTGC GGACGCTCAA GATCATGCTC
    AAGGTCTACT CCCGGGCCCT GCGGGAGGAG CTGCAATTCA GCCCCAAGGC CGTCAGCCGC
    CTGTTCCCAG GCGCAGACGA CCTGCTGGAG GTGCACAGCC ACTTCCTGTG TCGGCTGAAG
    GAGCGCCGCC TGGAGTCGCT GGAGGAGGGC AGTGCCCACA ACTACATCAT CCGGAAGATC
    GGGGACCTCC TGCTTCAGCA GTTTTCAGGT GAAAACGGAG AGAGGATGAA AGAAAAATAC
    AGCGTCTTTT GTAGCGGTCA CAACGATGCT GTCAGTCACT ACAAGTTGCT GCTGCAGCAA
    AACAAGAAAT TTCAAAACTT GATCAAGAGA ATCGGCAACT TCTCCATCGT GAGGCGGCTC
    GGCGTGCAGG AGTGTATCCT CCTGGTCACC CAACGCATCA CCAAATACCC TGTGCTGGTA
    GAGCGAATTA TCCAGAATAC AGAAGCTGGC TCCGAGGACC ACAGAGACCT GACCCAGGCC
    CTGAACCTCA TCAAAGACAT CATCTCACAA GTGGACGCCA GGGTCAGTGA GTGCGAGAAG
    GGCCAGCGCC TCAGGGAGAT CGCTGGGAAG ATGGACCTCA AGTCCTCCAG CAAGCTCAAA
    AATGGGCTGA CCTTCCGCAA GGAGGACATG CTGCAGAGGC AGCTCCACCT GGAGGGCACC
    CTGTGCTGGA AGACCACCTC TGGGCGCCTG AAAGACGTCC TCGCAGTCCT GTTGACAGAT
    GTGCTGCTGC TGCTCCAAGA AAAGGATCAG AAATACGTCT TTGCATCCGT GGACTCCAAG
    CGCCCCGTCA TCTCGCTGCA AAAGCTCATC GTGAGGGAGG TAGCCAACGA GGAGAAAGCC
    ATGTTTCTGA TCAGTGCCTC CCTGCATGGG CCCGAGATGT ACGAGATCTA TACCAGCTCG
    AAGGAAGACA GGAACACCTG GATGGCCCAC ATCCGGAGGG CCGTGGAGAG CTGCCCAGAC
    GAAGAGGAAG GGCCCTTCAG CGAGGCCGAA GAGGAAAAGA AGGTGGTCGA GACGCGTGCT
    GTGAGGCTGA GGGACTTCCA AGAGCGACTG AACAGGAAAG ACCAGCAGAT TGCACAGAGC
    CTGAGCGAGA AGCAGCAGAT CTACCTGGAG ATGGCAGAGA TGAGCGGGCT CGAGGACCTC
    GCCCTGTCTC GGCTTCTCTT CCGGGGAGGG GACCCCCCCG AGAACCTGCA GGGCGAGCTG
    ATTCTCAAGT CAGCCATGAG TGAGATCGAA GACATCCAGA GTTTCATCTG CAGGCGGCTG
    GGCAACGCCT ACTGCCAGGC AGAAGACGGG GGCAGCGCAG GCAGCCCAGG CAAGGACGGC
    AGTCTTCAGA AGAACATGTG CAGCAGTGAC CTCAGCCCCC GAGACTGGCA GGGCCTGGTG
    AGCAGCCTGG ACTCGAGGCC TGGAGACACC CAGGGGGCCC CCGAGGAAGC CTCAAAGGTG
    GCAGAGTCAC CAGGTGCAGA CCCCGGCCCC CACCCACCTG CGGTCCTGGA GTTGGAGCTC
    GTCCAGCGGA TCCAGACGCT GTCCCAGCTG CTCCTCAGCC TCCAGGTGGT CATCGCCCAG
    CAGGACAGCT ACGTGGAAAT TCAGAAAGCA GCCATCCAGG AGCGCGAGAA GCAGTTCCGG
    CTGCAGTCAA CACGCGGGAA CCTGCTGCTG GAACAGGAGC GGCAGCGCAA CTTTGAGAAG
    CAGCGAGAGG AGCTGGCAGG CGTGCAGAAG CTGCAGGGCC AGCTGCGGCT GGAGCAGCAA
    CGCTGGGAGC GCGAGCGGGA GCAGCAGCGC CGGGAGCTGG ACCAGGCCTC GGCGCGGCTG
    CAGCAGCGTG AGAGTGAGTT GCTGCGGCTG CAGGAGCGGC TGAACCAGGA GCGCGAGGAG
    CTGGAGCGCC AGCGCCAGGC CTACCAGCAT GACCTGGAGC GGCTGCGCGA GGCCCAGCGC
    GCCGTGGAGC GGGAGCGCGA GCGCCTAGAG GTGCTGCGCC GGCTCAAGAA GCAGAACACA
    GCGCCTGGGG CGCTGCCGCC GGAGGCACTG GCGGAGACCC AGCCTCTAAG CCACACTCCC
    AGCTTCAATG GGAATGGGCT GGATGGATCC ATGACCCTGA CCAAAGCACC AGGGCCCCGA
    CCCACCTCGC TGGTGTCCAG TGCGGGGTCC GAGTATGCCG AGCGCCCCGA GGTGGCCCGC
    AGGGACAGCG CCCCGGCCAA GAGCGAAGTG CCCATCCAGC TGCTGAGTGC CACCAACCAG
    ATCCAGAGGC AGGCAGCTGT GCAGCAGCAG ATTCCCACCA AGTTGGCAGC CTCCACCAAG
    GGCGGAAAGG ACAAGGGAGG CAAGAACCGG GGCTCCCAGC GCTGGGACAG CTCGGCCTCG
    TTTGACCTGA GGCAGCCGCT GCTTCTCAAC AAGCTCATGG GCAAAGAAGA GAACACCTCT
    CGGAACCGCC GCTCGCTGAG TCCCGTCCTG CCCAGCTCCA CGCCCCACCA AGACCCCAGC
    CTTCCAGCCC CGACCCCGGG CCCAGCCGAT GTCCCCACCG AGTCCCTCCC CCTCAAGACC
    TGGGGGCCAT CCTTTCCACC CGGGGCCCCG TCCTCCACAC CAGTCAACAA GGAAGAAACG
    GGCAAGGAAG ACGTCATCTT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90244
    Clone ID Related Accession (Same CDS sequence) XM_015464088.1 , XM_015472031.1 , XM_015472031.2
    Accession Version XM_015464088.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319574.1
    CDS276..3383
    Translation

    Target ORF information:

    RefSeq Version XM_015464088.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90244
    Clone ID Related Accession (Same CDS sequence) XM_015464088.1 , XM_015472031.1 , XM_015472031.2
    Accession Version XM_015472031.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327517.1
    CDS276..3383
    Translation

    Target ORF information:

    RefSeq Version XM_015472031.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90244
    Clone ID Related Accession (Same CDS sequence) XM_015464088.1 , XM_015472031.1 , XM_015472031.2
    Accession Version XM_015472031.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327517.1
    CDS184..3291
    Translation

    Target ORF information:

    RefSeq Version XM_015472031.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCCCTT ACCTGAGTTT CCTGAGGACC ATTTCTTTTC AGCCAGCAAT CTGTGCAGCA 
    TCCCTGAAGG AGCACCCGAG GGCCACCCTC CTGTCTGATG GCAGCCCACG CCCGACCAGG
    AATGTCGGCA TGACGGTCTC GCAGAAGGGG GGTCCACAGC CAGCACCCAG CCCAGCGGGA
    ACCGGGACAA GACTCGGACC ACTCACAGGA GAGATGGATG AAACTGATAC ACTGTTTCCG
    AAGCTCAAGC AGGTGGCCGA TGACTCGCTT TTTCTTACAT CTTCCAGCAC TGAGCCCATC
    TTTATAGAAG ACCCCTACAC TGCCTCGCTG AGAGGCGAGG TCGAAGCGGA CACCCACGAG
    TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG GACCCCGAGT ATGTGAAGAA ACAGAAGAGG
    GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT GAGCTGATGC AGACGGAGGC CCACCATGTG
    CGGACGCTCA AGATCATGCT CAAGGTCTAC TCCCGGGCCC TGCGGGAGGA GCTGCAATTC
    AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA GGCGCAGACG ACCTGCTGGA GGTGCACAGC
    CACTTCCTGT GTCGGCTGAA GGAGCGCCGC CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC
    AACTACATCA TCCGGAAGAT CGGGGACCTC CTGCTTCAGC AGTTTTCAGG TGAAAACGGA
    GAGAGGATGA AAGAAAAATA CAGCGTCTTT TGTAGCGGTC ACAACGATGC TGTCAGTCAC
    TACAAGTTGC TGCTGCAGCA AAACAAGAAA TTTCAAAACT TGATCAAGAG AATCGGCAAC
    TTCTCCATCG TGAGGCGGCT CGGCGTGCAG GAGTGTATCC TCCTGGTCAC CCAACGCATC
    ACCAAATACC CTGTGCTGGT AGAGCGAATT ATCCAGAATA CAGAAGCTGG CTCCGAGGAC
    CACAGAGACC TGACCCAGGC CCTGAACCTC ATCAAAGACA TCATCTCACA AGTGGACGCC
    AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC CTCAGGGAGA TCGCTGGGAA GATGGACCTC
    AAGTCCTCCA GCAAGCTCAA AAATGGGCTG ACCTTCCGCA AGGAGGACAT GCTGCAGAGG
    CAGCTCCACC TGGAGGGCAC CCTGTGCTGG AAGACCACCT CTGGGCGCCT GAAAGACGTC
    CTCGCAGTCC TGTTGACAGA TGTGCTGCTG CTGCTCCAAG AAAAGGATCA GAAATACGTC
    TTTGCATCCG TGGACTCCAA GCGCCCCGTC ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG
    GTAGCCAACG AGGAGAAAGC CATGTTTCTG ATCAGTGCCT CCCTGCATGG GCCCGAGATG
    TACGAGATCT ATACCAGCTC GAAGGAAGAC AGGAACACCT GGATGGCCCA CATCCGGAGG
    GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG
    AAGGTGGTCG AGACGCGTGC TGTGAGGCTG AGGGACTTCC AAGAGCGACT GAACAGGAAA
    GACCAGCAGA TTGCACAGAG CCTGAGCGAG AAGCAGCAGA TCTACCTGGA GATGGCAGAG
    ATGAGCGGGC TCGAGGACCT CGCCCTGTCT CGGCTTCTCT TCCGGGGAGG GGACCCCCCC
    GAGAACCTGC AGGGCGAGCT GATTCTCAAG TCAGCCATGA GTGAGATCGA AGACATCCAG
    AGTTTCATCT GCAGGCGGCT GGGCAACGCC TACTGCCAGG CAGAAGACGG GGGCAGCGCA
    GGCAGCCCAG GCAAGGACGG CAGTCTTCAG AAGAACATGT GCAGCAGTGA CCTCAGCCCC
    CGAGACTGGC AGGGCCTGGT GAGCAGCCTG GACTCGAGGC CTGGAGACAC CCAGGGGGCC
    CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA CCAGGTGCAG ACCCCGGCCC CCACCCACCT
    GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG ATCCAGACGC TGTCCCAGCT GCTCCTCAGC
    CTCCAGGTGG TCATCGCCCA GCAGGACAGC TACGTGGAAA TTCAGAAAGC AGCCATCCAG
    GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA ACACGCGGGA ACCTGCTGCT GGAACAGGAG
    CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC
    CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG
    GACCAGGCCT CGGCGCGGCT GCAGCAGCGT GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG
    CTGAACCAGG AGCGCGAGGA GCTGGAGCGC CAGCGCCAGG CCTACCAGCA TGACCTGGAG
    CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC
    CGGCTCAAGA AGCAGAACAC AGCGCCTGGG GCGCTGCCGC CGGAGGCACT GGCGGAGACC
    CAGCCTCTAA GCCACACTCC CAGCTTCAAT GGGAATGGGC TGGATGGATC CATGACCCTG
    ACCAAAGCAC CAGGGCCCCG ACCCACCTCG CTGGTGTCCA GTGCGGGGTC CGAGTATGCC
    GAGCGCCCCG AGGTGGCCCG CAGGGACAGC GCCCCGGCCA AGAGCGAAGT GCCCATCCAG
    CTGCTGAGTG CCACCAACCA GATCCAGAGG CAGGCAGCTG TGCAGCAGCA GATTCCCACC
    AAGTTGGCAG CCTCCACCAA GGGCGGAAAG GACAAGGGAG GCAAGAACCG GGGCTCCCAG
    CGCTGGGACA GCTCGGCCTC GTTTGACCTG AGGCAGCCGC TGCTTCTCAA CAAGCTCATG
    GGCAAAGAAG AGAACACCTC TCGGAACCGC CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC
    ACGCCCCACC AAGACCCCAG CCTTCCAGCC CCGACCCCGG GCCCAGCCGA TGTCCCCACC
    GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA TCCTTTCCAC CCGGGGCCCC GTCCTCCACA
    CCAGTCAACA AGGAAGAAAC GGGCAAGGAA GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010798247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796549.1
    CDS411..4448
    Translation

    Target ORF information:

    RefSeq Version XM_010798247.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010798245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796547.1
    CDS523..4560
    Translation

    Target ORF information:

    RefSeq Version XM_010798245.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806868.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805170.1
    CDS497..4534
    Translation

    Target ORF information:

    RefSeq Version XM_010806868.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806868.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806868.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805170.1
    CDS411..4448
    Translation

    Target ORF information:

    RefSeq Version XM_010806868.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806868.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806871.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805173.1
    CDS260..4297
    Translation

    Target ORF information:

    RefSeq Version XM_010806871.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806871.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806872.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805174.1
    CDS331..4368
    Translation

    Target ORF information:

    RefSeq Version XM_010806872.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806872.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806871.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805173.1
    CDS1756..5793
    Translation

    Target ORF information:

    RefSeq Version XM_010806871.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010798244.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796546.1
    CDS497..4534
    Translation

    Target ORF information:

    RefSeq Version XM_010798244.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010798246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796548.1
    CDS1756..5793
    Translation

    Target ORF information:

    RefSeq Version XM_010798246.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806870.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805172.1
    CDS523..4560
    Translation

    Target ORF information:

    RefSeq Version XM_010806870.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806872.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805174.1
    CDS411..4448
    Translation

    Target ORF information:

    RefSeq Version XM_010806872.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50571
    Clone ID Related Accession (Same CDS sequence) XM_010798247.2 , XM_010798245.2 , XM_010806868.2 , XM_010806868.3 , XM_010806871.3 , XM_010806872.3 , XM_010806871.2 , XM_010798244.2 , XM_010798246.2 , XM_010806870.2 , XM_010806872.2 , XM_010806870.3
    Accession Version XM_010806870.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 18 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805172.1
    CDS443..4480
    Translation

    Target ORF information:

    RefSeq Version XM_010806870.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 18 (ARHGEF18), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806870.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGATG AGCAGGAGGA TGGAATCCCA GGGCTGCTCG GCGGTAGCGA GAGTTCAGAG 
    GACCTCAGCC TGGATTTGGG GGCCCTTCAG GGCAGTGAGT ATCTCCAGGA CCTGGGCCTC
    GAAGTCCTTT CACACAGCCA GCCTGGGGGA GCCAGAGACA GTGGCCCCCC TAGTGAAGAA
    GCCCGAGGAG AAACGCCCCG AGGAGAAACG CCCCTCTCCA GCGCTGCGAG GTCCCAGGGC
    CTTGCTCGGA GACGCAGCTG GGAGAGGTCA AGGAGCTGCT CTGAGAGCCG GCAGAGGCTC
    AGCATCGAAT CCTCAGCTGT GAATGAGGGG CCCTTTCTGC CTCGGACACT GGCCAGCCTC
    GCTCTGAACC TGCCAGGAGA GGGCCTGCAG GCCTGGACCC AAGGGTGTCT GCCTGGGGGT
    GGAACCCCCG CAGAGCAACC AAGCAAGGAA TGTGACAGCC CCGAGAAAAG AGTGAGGTCA
    CGGTCTGTTC CTGTGTCCTT CGATGAGATC AGCTCCCTGG AAATCTTTCC AGCTTTGGAA
    GTGCCACCTC CAGCTGTTCA AGGCCTGGAC CCCCCGGTGC TGGAGTGCAT GGAAAAGGAT
    CATGTGGAGC CGAATCATGT GTTGATTGTC CAGCAGGTGC TTGAAGAACT CCGACAGTAC
    CATGGGGCCC GGCAGAGGGC TCGATTGTCG GTCTGCCCCG GAGGAGCAGC TTCGAACCTC
    ACCTGGTTTG AGTTCTTGTC TGAGAGTGAG GACAGCATGG GGAAGACGGA GAGAAGCGAC
    AGGGGCACCA AGGTGAAACG CAGCCTGAGC TCCCTGCGCA ATCGAGTCAC CAGGCAGAAG
    GAGAAGGGGA AGACCCCAGT GCATCAGAAG GACAAAGGTC AGGATGCGCG AGAGAGGAAA
    GAATGTATCA ACGGGCACCA GCTGGCTCGA GGAACCTTCT CTGGCCACTC CAGCTGCCCT
    ATGTGCGGCA AACCTTTTCT GAGCTCTGCA TCCCTGAAGG AGCACCCGAG GGCCACCCTC
    CTGTCTGATG GCAGCCCACG CCCGACCAGG AATGTCGGCA TGACGGTCTC GCAGAAGGGG
    GGTCCACAGC CAGCACCCAG CCCAGCGGGA ACCGGGACAA GACTCGGACC ACTCACAGGA
    GAGATGGATG AAACTGATAC ACTGTTTCCG AAGCTCAAGC AGGTGGCCGA TGACTCGCTT
    TTTCTTACAT CTTCCAGCAC TGAGCCCATC TTTATAGAAG ACCCCTACAC TGCCTCGCTG
    AGAGGCGAGG TCGAAGCGGA CACCCACGAG TTCGAGGCTG AGTCCTGGAG CCTCTCTGTG
    GACCCCGAGT ATGTGAAGAA ACAGAAGAGG GAGGTGGTGA AAAGGCAGGA CGTGCTTTAT
    GAGCTGATGC AGACGGAGGC CCACCATGTG CGGACGCTCA AGATCATGCT CAAGGTCTAC
    TCCCGGGCCC TGCGGGAGGA GCTGCAATTC AGCCCCAAGG CCGTCAGCCG CCTGTTCCCA
    GGCGCAGACG ACCTGCTGGA GGTGCACAGC CACTTCCTGT GTCGGCTGAA GGAGCGCCGC
    CTGGAGTCGC TGGAGGAGGG CAGTGCCCAC AACTACATCA TCCGGAAGAT CGGGGACCTC
    CTGCTTCAGC AGTTTTCAGG TGAAAACGGA GAGAGGATGA AAGAAAAATA CAGCGTCTTT
    TGTAGCGGTC ACAACGATGC TGTCAGTCAC TACAAGTTGC TGCTGCAGCA AAACAAGAAA
    TTTCAAAACT TGATCAAGAG AATCGGCAAC TTCTCCATCG TGAGGCGGCT CGGCGTGCAG
    GAGTGTATCC TCCTGGTCAC CCAACGCATC ACCAAATACC CTGTGCTGGT AGAGCGAATT
    ATCCAGAATA CAGAAGCTGG CTCCGAGGAC CACAGAGACC TGACCCAGGC CCTGAACCTC
    ATCAAAGACA TCATCTCACA AGTGGACGCC AGGGTCAGTG AGTGCGAGAA GGGCCAGCGC
    CTCAGGGAGA TCGCTGGGAA GATGGACCTC AAGTCCTCCA GCAAGCTCAA AAATGGGCTG
    ACCTTCCGCA AGGAGGACAT GCTGCAGAGG CAGCTCCACC TGGAGGGCAC CCTGTGCTGG
    AAGACCACCT CTGGGCGCCT GAAAGACGTC CTCGCAGTCC TGTTGACAGA TGTGCTGCTG
    CTGCTCCAAG AAAAGGATCA GAAATACGTC TTTGCATCCG TGGACTCCAA GCGCCCCGTC
    ATCTCGCTGC AAAAGCTCAT CGTGAGGGAG GTAGCCAACG AGGAGAAAGC CATGTTTCTG
    ATCAGTGCCT CCCTGCATGG GCCCGAGATG TACGAGATCT ATACCAGCTC GAAGGAAGAC
    AGGAACACCT GGATGGCCCA CATCCGGAGG GCCGTGGAGA GCTGCCCAGA CGAAGAGGAA
    GGGCCCTTCA GCGAGGCCGA AGAGGAAAAG AAGGTGGTCG AGACGCGTGC TGTGAGGCTG
    AGGGACTTCC AAGAGCGACT GAACAGGAAA GACCAGCAGA TTGCACAGAG CCTGAGCGAG
    AAGCAGCAGA TCTACCTGGA GATGGCAGAG ATGAGCGGGC TCGAGGACCT CGCCCTGTCT
    CGGCTTCTCT TCCGGGGAGG GGACCCCCCC GAGAACCTGC AGGGCGAGCT GATTCTCAAG
    TCAGCCATGA GTGAGATCGA AGACATCCAG AGTTTCATCT GCAGGCGGCT GGGCAACGCC
    TACTGCCAGG CAGAAGACGG GGGCAGCGCA GGCAGCCCAG GCAAGGACGG CAGTCTTCAG
    AAGAACATGT GCAGCAGTGA CCTCAGCCCC CGAGACTGGC AGGGCCTGGT GAGCAGCCTG
    GACTCGAGGC CTGGAGACAC CCAGGGGGCC CCCGAGGAAG CCTCAAAGGT GGCAGAGTCA
    CCAGGTGCAG ACCCCGGCCC CCACCCACCT GCGGTCCTGG AGTTGGAGCT CGTCCAGCGG
    ATCCAGACGC TGTCCCAGCT GCTCCTCAGC CTCCAGGTGG TCATCGCCCA GCAGGACAGC
    TACGTGGAAA TTCAGAAAGC AGCCATCCAG GAGCGCGAGA AGCAGTTCCG GCTGCAGTCA
    ACACGCGGGA ACCTGCTGCT GGAACAGGAG CGGCAGCGCA ACTTTGAGAA GCAGCGAGAG
    GAGCTGGCAG GCGTGCAGAA GCTGCAGGGC CAGCTGCGGC TGGAGCAGCA ACGCTGGGAG
    CGCGAGCGGG AGCAGCAGCG CCGGGAGCTG GACCAGGCCT CGGCGCGGCT GCAGCAGCGT
    GAGAGTGAGT TGCTGCGGCT GCAGGAGCGG CTGAACCAGG AGCGCGAGGA GCTGGAGCGC
    CAGCGCCAGG CCTACCAGCA TGACCTGGAG CGGCTGCGCG AGGCCCAGCG CGCCGTGGAG
    CGGGAGCGCG AGCGCCTAGA GGTGCTGCGC CGGCTCAAGA AGCAGAACAC AGCGCCTGGG
    GCGCTGCCGC CGGAGGCACT GGCGGAGACC CAGCCTCTAA GCCACACTCC CAGCTTCAAT
    GGGAATGGGC TGGATGGATC CATGACCCTG ACCAAAGCAC CAGGGCCCCG ACCCACCTCG
    CTGGTGTCCA GTGCGGGGTC CGAGTATGCC GAGCGCCCCG AGGTGGCCCG CAGGGACAGC
    GCCCCGGCCA AGAGCGAAGT GCCCATCCAG CTGCTGAGTG CCACCAACCA GATCCAGAGG
    CAGGCAGCTG TGCAGCAGCA GATTCCCACC AAGTTGGCAG CCTCCACCAA GGGCGGAAAG
    GACAAGGGAG GCAAGAACCG GGGCTCCCAG CGCTGGGACA GCTCGGCCTC GTTTGACCTG
    AGGCAGCCGC TGCTTCTCAA CAAGCTCATG GGCAAAGAAG AGAACACCTC TCGGAACCGC
    CGCTCGCTGA GTCCCGTCCT GCCCAGCTCC ACGCCCCACC AAGACCCCAG CCTTCCAGCC
    CCGACCCCGG GCCCAGCCGA TGTCCCCACC GAGTCCCTCC CCCTCAAGAC CTGGGGGCCA
    TCCTTTCCAC CCGGGGCCCC GTCCTCCACA CCAGTCAACA AGGAAGAAAC GGGCAAGGAA
    GACGTCATCT TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.