MAN2A1 cDNA ORF clone, Bos taurus(Cattle)

The following MAN2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236047 XM_024994256.1
Latest version!
Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa239243 NM_001205886.3
Latest version!
Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236046 XM_024994255.1
Latest version!
Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa01592 NM_001205886.2
Latest version!
Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa01592 NM_001205886.1 Bos taurus mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236047
    Clone ID Related Accession (Same CDS sequence) XM_024994256.1
    Accession Version XM_024994256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGCAGA TAAGAGGTTG GTTGAAGACC TTTGATGACT ACTTTAGAGA GAAGACTCAG 
    TATATTTTTA ATAATATGGT TGTCAAGTTG AAAGAAGACC CACGGAGAAA ATTTATATGG
    TCTGAGATCT CTTACCTTTC GAAGTGGTGG GAAGCTATAG ATGTTCAGAA GAAGGATGCT
    GTTAAAAGTT TGCTAGAAAA CGGTCAGTTT GAAATTGTGA CAGGTGGCTG GGTAATGCCT
    GATGAAGCTG GTGCACATTA TTTTGCCATA ATTGACCAAC TAATTGAAGG ACATCAGTGG
    CTGGAGAAGA ATCTAGGAGT GAAACCTCGG TCTGGTTGGG CTATCGACCC ATTTGGACAT
    TCACCAACAA TGGCGTATCT TCTAAAACGT GCTGGATTTT CTCACATGCT TATTCAGAGA
    GTTCATTATG CAGTTAAAAA ACACTTTGCA TTGCATAAAA CACTGGAGTT TTTTTGGAGA
    CAAAATTGGG ATCTGGGATC TGTCACAGAC ATTTTTTGCC ATATGATGCC CTTCTACAGC
    TACGACATCC CCCACACTTG TGGGCCTGAT CCTAAAATAT GCTGCCAGTT TGATTTTAAA
    CGGCTTCCTG GAGGCAGATA CGGTTGTCCC TGGGGAGTCC CCCCAGAAAC AATATATCCT
    GGAAATCTCC AGAACAGGGC CGAGATGCTC CTGGACCAGT ACCGAAAGAA GTCAGAGCTC
    TTCCGTACCA CAGTGGTCCT GGCCCCGCTG GGAGACGATT TCCGCTACAC CGAACGCACC
    GAATGGGATC ACCAGTTCAA GAATTACCAG CTGCTTTTTG ATTATATGAA CTCTCATTCT
    CAGTATAATG TTAAGATACA GTTTGGGACT TTATCAGATT ATTTTGATGC ACTAGAAAAA
    GAAGCTTCAG CCAGTAGTAG GAATAGCCAA TCAATATTCC CTGTTGTAAG TGGAGATTTT
    TTCACTTATG CTGACCGAGA TGATCATTAC TGGAGTGGCT ATTTTACATC CAGGCCCTTT
    TACAAACGAA TGGATAGGAT ATTGGAATCC CATTTAAGGG CTGCTGAAAT TCTTTACTAT
    TTCGCCATGA AACAAGCTAA GCAGTACAAG ATAAGTAAAT TTCTTTCATC ATCCCATTAT
    GTGATGCTGA CAGAAGCAAG ACGAAATTTG GGACTATTTC AACATCATGA TGCTATCACA
    GGAACTGCAA AAGATTGGGT GGTTGTGGAT TATGGTACCA GACTCTTTCA TTCACTGATG
    AATTTAAAGA AGATAATTGG ACATTCTGCA CTGCTGCTTA TTTTGAAGGA CAAACACTCA
    TACGACTCTT ATTCTTCGGA TACCTTCCTA GAAATGGATT TGAAACAAAG AACGCAAAGT
    TCTCTACCAC AAAAAAATGT AATAAGTCTG AGCGCAGAGC CAAGGTATCT CGTGGTCTAT
    AACCCTTCAG AACAAGCTCG AAGCTCTGTA GTCTCAGTCT GTGTGAGCTC ATCCGCAGCA
    CAAGTGTCCT CTGCTTCAGG AAAGCCTGTG GAAATCCAAA TGAGTGCAGT GTGGGACACA
    GCAACAACCG TCTCACAGAC AGCCTACGAG ATATCTTTTC TAGCAGAGAT GCCACCACTG
    GGACTGAAAG TGTATAAGAT TTTGGAAACA TCAAGTTCAA ATCCACATTT AGCCAAATAT
    GACCTATATA ATGGTAATAT AGCAAATGAA GGAATTTTTA ACATGAATAA TATAAAAAGT
    GCTCAAGAAG CTATAACTCT AGAGAACTCC TTTATTAGGC TGCGCTTTGG TCAATCTGGG
    CTTATGGAGG AAATGCTAAA TAGAGGAGAT GGTAAGAGTC TTGAGGTGAC AGTACAGTTT
    TCCTGGTATG GAACCACAAG TAAAAGGGAT AAAAGTGGTG CCTACCTCTT CTTACCCGAC
    GGGGAGGCCA AGCCTTACGT TTACACAACA CCACCCTTTG TCAGAGTGCA GCGTGGAAGG
    TTTTATTCAG ACGTGACTTG CTTTTTTGAA CATGTTACCC ACAGAGTTCG ACTGTACAAC
    ATACATGGAG TAGAAGGACA GTCTGTGGAG ATTTCCAATA TTGTAGACAT CCGAAAAGAG
    CATAACTATG AGATTGCAAT GAGAATTTCT TCCAGCATCA ACAGCCAAAA CAGATTTTAT
    ACTGACCTAA ATGGATACCA GATTCAGCCT AGAGTGACGA TGAGCAAATT GCCTCTCCAG
    GCGAACGTCT ACCCCATGAC TACGATGGCG TACATTCAGG ACGCCGAGCG CCGCTTGACT
    CTGCTCTCGG CTCAGTCTTT GGGTGTTTCG AGTCTGAAGA GCGGTCAGAT TGAAGTTATC
    ATGGATCGAA GACTCATGCA AGATGATAAT CGTGGCCTTG AGCAAGGTGT CCATGATAAC
    AAGATTACAG CTAATTTATT TCGAATACTA CTAGAAAAGA GAAGTGTCGT GAATATGGAA
    GAAGACCAGA AGGCAGTCAG TTACCCTTCC CTCCTTAGCC ACATAACGTC TTCTTTCCTG
    AATCACCCTG TCTTCCCAAT GACAGAAAAG GTCCCCGTGC CCACCCTTCA GCTGCTGGGT
    GAATTCTCTC CCTTGATGGC ACCCTTGCCC TGTGACATTC ACCTGGTTAA TCTGAGGACA
    GTGCAGTCGA AGGTGGATGG CAAGCATTCC AACGAGGCCG CCTTGATCCT CCACAGGAAG
    GGGTTTGACT GCCGCTTCTC TAGCAGAGAC ACCGGGCTGC TGTGTTCCAC AACCCAGGGA
    AAGATCCTGG TCCAGAAGCT CTTCAGCAAG TTCACTGTTG TGAGCATCAC ACCTTCGTCA
    TTATCCCTGA TGCATCCCCC TCCAGACGCC CGAAATATAA GTGAGATCAA CTTGAGTCCC
    ATGGAAATCA GCACATTCCG AATCCACTTG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850024.1
    CDS16..2931
    Translation

    Target ORF information:

    RefSeq Version XM_024994256.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGCAGA TAAGAGGTTG GTTGAAGACC TTTGATGACT ACTTTAGAGA GAAGACTCAG 
    TATATTTTTA ATAATATGGT TGTCAAGTTG AAAGAAGACC CACGGAGAAA ATTTATATGG
    TCTGAGATCT CTTACCTTTC GAAGTGGTGG GAAGCTATAG ATGTTCAGAA GAAGGATGCT
    GTTAAAAGTT TGCTAGAAAA CGGTCAGTTT GAAATTGTGA CAGGTGGCTG GGTAATGCCT
    GATGAAGCTG GTGCACATTA TTTTGCCATA ATTGACCAAC TAATTGAAGG ACATCAGTGG
    CTGGAGAAGA ATCTAGGAGT GAAACCTCGG TCTGGTTGGG CTATCGACCC ATTTGGACAT
    TCACCAACAA TGGCGTATCT TCTAAAACGT GCTGGATTTT CTCACATGCT TATTCAGAGA
    GTTCATTATG CAGTTAAAAA ACACTTTGCA TTGCATAAAA CACTGGAGTT TTTTTGGAGA
    CAAAATTGGG ATCTGGGATC TGTCACAGAC ATTTTTTGCC ATATGATGCC CTTCTACAGC
    TACGACATCC CCCACACTTG TGGGCCTGAT CCTAAAATAT GCTGCCAGTT TGATTTTAAA
    CGGCTTCCTG GAGGCAGATA CGGTTGTCCC TGGGGAGTCC CCCCAGAAAC AATATATCCT
    GGAAATCTCC AGAACAGGGC CGAGATGCTC CTGGACCAGT ACCGAAAGAA GTCAGAGCTC
    TTCCGTACCA CAGTGGTCCT GGCCCCGCTG GGAGACGATT TCCGCTACAC CGAACGCACC
    GAATGGGATC ACCAGTTCAA GAATTACCAG CTGCTTTTTG ATTATATGAA CTCTCATTCT
    CAGTATAATG TTAAGATACA GTTTGGGACT TTATCAGATT ATTTTGATGC ACTAGAAAAA
    GAAGCTTCAG CCAGTAGTAG GAATAGCCAA TCAATATTCC CTGTTGTAAG TGGAGATTTT
    TTCACTTATG CTGACCGAGA TGATCATTAC TGGAGTGGCT ATTTTACATC CAGGCCCTTT
    TACAAACGAA TGGATAGGAT ATTGGAATCC CATTTAAGGG CTGCTGAAAT TCTTTACTAT
    TTCGCCATGA AACAAGCTAA GCAGTACAAG ATAAGTAAAT TTCTTTCATC ATCCCATTAT
    GTGATGCTGA CAGAAGCAAG ACGAAATTTG GGACTATTTC AACATCATGA TGCTATCACA
    GGAACTGCAA AAGATTGGGT GGTTGTGGAT TATGGTACCA GACTCTTTCA TTCACTGATG
    AATTTAAAGA AGATAATTGG ACATTCTGCA CTGCTGCTTA TTTTGAAGGA CAAACACTCA
    TACGACTCTT ATTCTTCGGA TACCTTCCTA GAAATGGATT TGAAACAAAG AACGCAAAGT
    TCTCTACCAC AAAAAAATGT AATAAGTCTG AGCGCAGAGC CAAGGTATCT CGTGGTCTAT
    AACCCTTCAG AACAAGCTCG AAGCTCTGTA GTCTCAGTCT GTGTGAGCTC ATCCGCAGCA
    CAAGTGTCCT CTGCTTCAGG AAAGCCTGTG GAAATCCAAA TGAGTGCAGT GTGGGACACA
    GCAACAACCG TCTCACAGAC AGCCTACGAG ATATCTTTTC TAGCAGAGAT GCCACCACTG
    GGACTGAAAG TGTATAAGAT TTTGGAAACA TCAAGTTCAA ATCCACATTT AGCCAAATAT
    GACCTATATA ATGGTAATAT AGCAAATGAA GGAATTTTTA ACATGAATAA TATAAAAAGT
    GCTCAAGAAG CTATAACTCT AGAGAACTCC TTTATTAGGC TGCGCTTTGG TCAATCTGGG
    CTTATGGAGG AAATGCTAAA TAGAGGAGAT GGTAAGAGTC TTGAGGTGAC AGTACAGTTT
    TCCTGGTATG GAACCACAAG TAAAAGGGAT AAAAGTGGTG CCTACCTCTT CTTACCCGAC
    GGGGAGGCCA AGCCTTACGT TTACACAACA CCACCCTTTG TCAGAGTGCA GCGTGGAAGG
    TTTTATTCAG ACGTGACTTG CTTTTTTGAA CATGTTACCC ACAGAGTTCG ACTGTACAAC
    ATACATGGAG TAGAAGGACA GTCTGTGGAG ATTTCCAATA TTGTAGACAT CCGAAAAGAG
    CATAACTATG AGATTGCAAT GAGAATTTCT TCCAGCATCA ACAGCCAAAA CAGATTTTAT
    ACTGACCTAA ATGGATACCA GATTCAGCCT AGAGTGACGA TGAGCAAATT GCCTCTCCAG
    GCGAACGTCT ACCCCATGAC TACGATGGCG TACATTCAGG ACGCCGAGCG CCGCTTGACT
    CTGCTCTCGG CTCAGTCTTT GGGTGTTTCG AGTCTGAAGA GCGGTCAGAT TGAAGTTATC
    ATGGATCGAA GACTCATGCA AGATGATAAT CGTGGCCTTG AGCAAGGTGT CCATGATAAC
    AAGATTACAG CTAATTTATT TCGAATACTA CTAGAAAAGA GAAGTGTCGT GAATATGGAA
    GAAGACCAGA AGGCAGTCAG TTACCCTTCC CTCCTTAGCC ACATAACGTC TTCTTTCCTG
    AATCACCCTG TCTTCCCAAT GACAGAAAAG GTCCCCGTGC CCACCCTTCA GCTGCTGGGT
    GAATTCTCTC CCTTGATGGC ACCCTTGCCC TGTGACATTC ACCTGGTTAA TCTGAGGACA
    GTGCAGTCGA AGGTGGATGG CAAGCATTCC AACGAGGCCG CCTTGATCCT CCACAGGAAG
    GGGTTTGACT GCCGCTTCTC TAGCAGAGAC ACCGGGCTGC TGTGTTCCAC AACCCAGGGA
    AAGATCCTGG TCCAGAAGCT CTTCAGCAAG TTCACTGTTG TGAGCATCAC ACCTTCGTCA
    TTATCCCTGA TGCATCCCCC TCCAGACGCC CGAAATATAA GTGAGATCAA CTTGAGTCCC
    ATGGAAATCA GCACATTCCG AATCCACTTG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239243
    Clone ID Related Accession (Same CDS sequence) NM_001205886.3
    Accession Version NM_001205886.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000007.1. On Dec 20, 2018 this sequence version replaced NM_001205886.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.26643.1, SRR5571261.3853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAGAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192815.2
    CDS1..3438
    Translation

    Target ORF information:

    RefSeq Version NM_001205886.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAGAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236046
    Clone ID Related Accession (Same CDS sequence) XM_024994255.1
    Accession Version XM_024994255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3180bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGATGTT GGATGTTTAC AGTCTAATTC CTTTTGACAA TCCCGATGGT
    GGAGTTTGGA AGCAAGGATT TGACATCACT TATAAGCCTG ATGAATGGGA CACTAAGCCC
    CTTCAAGTCT TCGTGGTGCC TCACTCCCAT AATGACCCAG GTTGGTTGAA GACCTTTGAT
    GACTACTTTA GAGAGAAGAC TCAGTATATT TTTAATAATA TGGTTGTCAA GTTGAAAGAA
    GACCCACGGA GAAAATTTAT ATGGTCTGAG ATCTCTTACC TTTCGAAGTG GTGGGAAGCT
    ATAGATGTTC AGAAGAAGGA TGCTGTTAAA AGTTTGCTAG AAAACGGTCA GTTTGAAATT
    GTGACAGGTG GCTGGGTAAT GCCTGATGAA GCTGGTGCAC ATTATTTTGC CATAATTGAC
    CAACTAATTG AAGGACATCA GTGGCTGGAG AAGAATCTAG GAGTGAAACC TCGGTCTGGT
    TGGGCTATCG ACCCATTTGG ACATTCACCA ACAATGGCGT ATCTTCTAAA ACGTGCTGGA
    TTTTCTCACA TGCTTATTCA GAGAGTTCAT TATGCAGTTA AAAAACACTT TGCATTGCAT
    AAAACACTGG AGTTTTTTTG GAGACAAAAT TGGGATCTGG GATCTGTCAC AGACATTTTT
    TGCCATATGA TGCCCTTCTA CAGCTACGAC ATCCCCCACA CTTGTGGGCC TGATCCTAAA
    ATATGCTGCC AGTTTGATTT TAAACGGCTT CCTGGAGGCA GATACGGTTG TCCCTGGGGA
    GTCCCCCCAG AAACAATATA TCCTGGAAAT CTCCAGAACA GGGCCGAGAT GCTCCTGGAC
    CAGTACCGAA AGAAGTCAGA GCTCTTCCGT ACCACAGTGG TCCTGGCCCC GCTGGGAGAC
    GATTTCCGCT ACACCGAACG CACCGAATGG GATCACCAGT TCAAGAATTA CCAGCTGCTT
    TTTGATTATA TGAACTCTCA TTCTCAGTAT AATGTTAAGA TACAGTTTGG GACTTTATCA
    GATTATTTTG ATGCACTAGA AAAAGAAGCT TCAGCCAGTA GTAGGAATAG CCAATCAATA
    TTCCCTGTTG TAAGTGGAGA TTTTTTCACT TATGCTGACC GAGATGATCA TTACTGGAGT
    GGCTATTTTA CATCCAGGCC CTTTTACAAA CGAATGGATA GGATATTGGA ATCCCATTTA
    AGGGCTGCTG AAATTCTTTA CTATTTCGCC ATGAAACAAG CTAAGCAGTA CAAGATAAGT
    AAATTTCTTT CATCATCCCA TTATGTGATG CTGACAGAAG CAAGACGAAA TTTGGGACTA
    TTTCAACATC ATGATGCTAT CACAGGAACT GCAAAAGATT GGGTGGTTGT GGATTATGGT
    ACCAGACTCT TTCATTCACT GATGAATTTA AAGAAGATAA TTGGACATTC TGCACTGCTG
    CTTATTTTGA AGGACAAACA CTCATACGAC TCTTATTCTT CGGATACCTT CCTAGAAATG
    GATTTGAAAC AAAGAACGCA AAGTTCTCTA CCACAAAAAA ATGTAATAAG TCTGAGCGCA
    GAGCCAAGGT ATCTCGTGGT CTATAACCCT TCAGAACAAG CTCGAAGCTC TGTAGTCTCA
    GTCTGTGTGA GCTCATCCGC AGCACAAGTG TCCTCTGCTT CAGGAAAGCC TGTGGAAATC
    CAAATGAGTG CAGTGTGGGA CACAGCAACA ACCGTCTCAC AGACAGCCTA CGAGATATCT
    TTTCTAGCAG AGATGCCACC ACTGGGACTG AAAGTGTATA AGATTTTGGA AACATCAAGT
    TCAAATCCAC ATTTAGCCAA ATATGACCTA TATAATGGTA ATATAGCAAA TGAAGGAATT
    TTTAACATGA ATAATATAAA AAGTGCTCAA GAAGCTATAA CTCTAGAGAA CTCCTTTATT
    AGGCTGCGCT TTGGTCAATC TGGGCTTATG GAGGAAATGC TAAATAGAGG AGATGGTAAG
    AGTCTTGAGG TGACAGTACA GTTTTCCTGG TATGGAACCA CAAGTAAAAG GGATAAAAGT
    GGTGCCTACC TCTTCTTACC CGACGGGGAG GCCAAGCCTT ACGTTTACAC AACACCACCC
    TTTGTCAGAG TGCAGCGTGG AAGGTTTTAT TCAGACGTGA CTTGCTTTTT TGAACATGTT
    ACCCACAGAG TTCGACTGTA CAACATACAT GGAGTAGAAG GACAGTCTGT GGAGATTTCC
    AATATTGTAG ACATCCGAAA AGAGCATAAC TATGAGATTG CAATGAGAAT TTCTTCCAGC
    ATCAACAGCC AAAACAGATT TTATACTGAC CTAAATGGAT ACCAGATTCA GCCTAGAGTG
    ACGATGAGCA AATTGCCTCT CCAGGCGAAC GTCTACCCCA TGACTACGAT GGCGTACATT
    CAGGACGCCG AGCGCCGCTT GACTCTGCTC TCGGCTCAGT CTTTGGGTGT TTCGAGTCTG
    AAGAGCGGTC AGATTGAAGT TATCATGGAT CGAAGACTCA TGCAAGATGA TAATCGTGGC
    CTTGAGCAAG GTGTCCATGA TAACAAGATT ACAGCTAATT TATTTCGAAT ACTACTAGAA
    AAGAGAAGTG TCGTGAATAT GGAAGAAGAC CAGAAGGCAG TCAGTTACCC TTCCCTCCTT
    AGCCACATAA CGTCTTCTTT CCTGAATCAC CCTGTCTTCC CAATGACAGA AAAGGTCCCC
    GTGCCCACCC TTCAGCTGCT GGGTGAATTC TCTCCCTTGA TGGCACCCTT GCCCTGTGAC
    ATTCACCTGG TTAATCTGAG GACAGTGCAG TCGAAGGTGG ATGGCAAGCA TTCCAACGAG
    GCCGCCTTGA TCCTCCACAG GAAGGGGTTT GACTGCCGCT TCTCTAGCAG AGACACCGGG
    CTGCTGTGTT CCACAACCCA GGGAAAGATC CTGGTCCAGA AGCTCTTCAG CAAGTTCACT
    GTTGTGAGCA TCACACCTTC GTCATTATCC CTGATGCATC CCCCTCCAGA CGCCCGAAAT
    ATAAGTGAGA TCAACTTGAG TCCCATGGAA ATCAGCACAT TCCGAATCCA CTTGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850023.1
    CDS167..3346
    Translation

    Target ORF information:

    RefSeq Version XM_024994255.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGATGTT GGATGTTTAC AGTCTAATTC CTTTTGACAA TCCCGATGGT
    GGAGTTTGGA AGCAAGGATT TGACATCACT TATAAGCCTG ATGAATGGGA CACTAAGCCC
    CTTCAAGTCT TCGTGGTGCC TCACTCCCAT AATGACCCAG GTTGGTTGAA GACCTTTGAT
    GACTACTTTA GAGAGAAGAC TCAGTATATT TTTAATAATA TGGTTGTCAA GTTGAAAGAA
    GACCCACGGA GAAAATTTAT ATGGTCTGAG ATCTCTTACC TTTCGAAGTG GTGGGAAGCT
    ATAGATGTTC AGAAGAAGGA TGCTGTTAAA AGTTTGCTAG AAAACGGTCA GTTTGAAATT
    GTGACAGGTG GCTGGGTAAT GCCTGATGAA GCTGGTGCAC ATTATTTTGC CATAATTGAC
    CAACTAATTG AAGGACATCA GTGGCTGGAG AAGAATCTAG GAGTGAAACC TCGGTCTGGT
    TGGGCTATCG ACCCATTTGG ACATTCACCA ACAATGGCGT ATCTTCTAAA ACGTGCTGGA
    TTTTCTCACA TGCTTATTCA GAGAGTTCAT TATGCAGTTA AAAAACACTT TGCATTGCAT
    AAAACACTGG AGTTTTTTTG GAGACAAAAT TGGGATCTGG GATCTGTCAC AGACATTTTT
    TGCCATATGA TGCCCTTCTA CAGCTACGAC ATCCCCCACA CTTGTGGGCC TGATCCTAAA
    ATATGCTGCC AGTTTGATTT TAAACGGCTT CCTGGAGGCA GATACGGTTG TCCCTGGGGA
    GTCCCCCCAG AAACAATATA TCCTGGAAAT CTCCAGAACA GGGCCGAGAT GCTCCTGGAC
    CAGTACCGAA AGAAGTCAGA GCTCTTCCGT ACCACAGTGG TCCTGGCCCC GCTGGGAGAC
    GATTTCCGCT ACACCGAACG CACCGAATGG GATCACCAGT TCAAGAATTA CCAGCTGCTT
    TTTGATTATA TGAACTCTCA TTCTCAGTAT AATGTTAAGA TACAGTTTGG GACTTTATCA
    GATTATTTTG ATGCACTAGA AAAAGAAGCT TCAGCCAGTA GTAGGAATAG CCAATCAATA
    TTCCCTGTTG TAAGTGGAGA TTTTTTCACT TATGCTGACC GAGATGATCA TTACTGGAGT
    GGCTATTTTA CATCCAGGCC CTTTTACAAA CGAATGGATA GGATATTGGA ATCCCATTTA
    AGGGCTGCTG AAATTCTTTA CTATTTCGCC ATGAAACAAG CTAAGCAGTA CAAGATAAGT
    AAATTTCTTT CATCATCCCA TTATGTGATG CTGACAGAAG CAAGACGAAA TTTGGGACTA
    TTTCAACATC ATGATGCTAT CACAGGAACT GCAAAAGATT GGGTGGTTGT GGATTATGGT
    ACCAGACTCT TTCATTCACT GATGAATTTA AAGAAGATAA TTGGACATTC TGCACTGCTG
    CTTATTTTGA AGGACAAACA CTCATACGAC TCTTATTCTT CGGATACCTT CCTAGAAATG
    GATTTGAAAC AAAGAACGCA AAGTTCTCTA CCACAAAAAA ATGTAATAAG TCTGAGCGCA
    GAGCCAAGGT ATCTCGTGGT CTATAACCCT TCAGAACAAG CTCGAAGCTC TGTAGTCTCA
    GTCTGTGTGA GCTCATCCGC AGCACAAGTG TCCTCTGCTT CAGGAAAGCC TGTGGAAATC
    CAAATGAGTG CAGTGTGGGA CACAGCAACA ACCGTCTCAC AGACAGCCTA CGAGATATCT
    TTTCTAGCAG AGATGCCACC ACTGGGACTG AAAGTGTATA AGATTTTGGA AACATCAAGT
    TCAAATCCAC ATTTAGCCAA ATATGACCTA TATAATGGTA ATATAGCAAA TGAAGGAATT
    TTTAACATGA ATAATATAAA AAGTGCTCAA GAAGCTATAA CTCTAGAGAA CTCCTTTATT
    AGGCTGCGCT TTGGTCAATC TGGGCTTATG GAGGAAATGC TAAATAGAGG AGATGGTAAG
    AGTCTTGAGG TGACAGTACA GTTTTCCTGG TATGGAACCA CAAGTAAAAG GGATAAAAGT
    GGTGCCTACC TCTTCTTACC CGACGGGGAG GCCAAGCCTT ACGTTTACAC AACACCACCC
    TTTGTCAGAG TGCAGCGTGG AAGGTTTTAT TCAGACGTGA CTTGCTTTTT TGAACATGTT
    ACCCACAGAG TTCGACTGTA CAACATACAT GGAGTAGAAG GACAGTCTGT GGAGATTTCC
    AATATTGTAG ACATCCGAAA AGAGCATAAC TATGAGATTG CAATGAGAAT TTCTTCCAGC
    ATCAACAGCC AAAACAGATT TTATACTGAC CTAAATGGAT ACCAGATTCA GCCTAGAGTG
    ACGATGAGCA AATTGCCTCT CCAGGCGAAC GTCTACCCCA TGACTACGAT GGCGTACATT
    CAGGACGCCG AGCGCCGCTT GACTCTGCTC TCGGCTCAGT CTTTGGGTGT TTCGAGTCTG
    AAGAGCGGTC AGATTGAAGT TATCATGGAT CGAAGACTCA TGCAAGATGA TAATCGTGGC
    CTTGAGCAAG GTGTCCATGA TAACAAGATT ACAGCTAATT TATTTCGAAT ACTACTAGAA
    AAGAGAAGTG TCGTGAATAT GGAAGAAGAC CAGAAGGCAG TCAGTTACCC TTCCCTCCTT
    AGCCACATAA CGTCTTCTTT CCTGAATCAC CCTGTCTTCC CAATGACAGA AAAGGTCCCC
    GTGCCCACCC TTCAGCTGCT GGGTGAATTC TCTCCCTTGA TGGCACCCTT GCCCTGTGAC
    ATTCACCTGG TTAATCTGAG GACAGTGCAG TCGAAGGTGG ATGGCAAGCA TTCCAACGAG
    GCCGCCTTGA TCCTCCACAG GAAGGGGTTT GACTGCCGCT TCTCTAGCAG AGACACCGGG
    CTGCTGTGTT CCACAACCCA GGGAAAGATC CTGGTCCAGA AGCTCTTCAG CAAGTTCACT
    GTTGTGAGCA TCACACCTTC GTCATTATCC CTGATGCATC CCCCTCCAGA CGCCCGAAAT
    ATAAGTGAGA TCAACTTGAG TCCCATGGAA ATCAGCACAT TCCGAATCCA CTTGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01592
    Clone ID Related Accession (Same CDS sequence) NM_001205886.2 , NM_001205886.1
    Accession Version NM_001205886.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02021699.1, CB452086.1 and HX937636.1. On Aug 24, 2017 this sequence version replaced NM_001205886.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403641 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192815.1
    CDS1..3438
    Translation

    Target ORF information:

    RefSeq Version NM_001205886.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01592
    Clone ID Related Accession (Same CDS sequence) NM_001205886.2 , NM_001205886.1
    Accession Version NM_001205886.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-04-23
    Organism Bos taurus(Cattle)
    Product alpha-mannosidase 2
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03039677.1, AAFC03039678.1, AAFC03057257.1 and AAFC03057258.1. On Apr 20, 2011 this sequence version replaced XM_599803.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192815.1
    CDS1..3438
    Translation

    Target ORF information:

    RefSeq Version NM_001205886.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG
    GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC
    TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT
    CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT
    TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT
    TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT
    GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT
    TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG
    GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT
    TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA
    AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT
    TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA
    GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT
    CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA
    AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA
    TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT
    TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA
    TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG
    GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC
    CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC
    AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA
    CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT
    AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA
    GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG
    ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT
    AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA
    AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG
    GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT
    GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG
    GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT
    GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT
    CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA
    GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG
    ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG
    ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT
    ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT
    CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA
    AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA
    AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC
    GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT
    TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA
    CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA
    ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC
    CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG
    ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT
    TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG
    CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA
    TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC
    AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA
    ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG
    GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT
    GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC
    TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG
    CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC
    CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC
    CGAATCCACT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.