MAN2A1 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2A1
Entrez Gene ID	521539
Full Name	mannosidase alpha class 2A member 1
General protein information	Preferred Names mannosidase alpha class 2A member 1 Names alpha-mannosidase 2
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205886.2	NP_001192815.1	alpha-mannosidase 2
XM_024994256.1	XP_024850024.1	alpha-mannosidase 2 isoform X2
XM_024994255.1	XP_024850023.1	alpha-mannosidase 2 isoform X1
NM_001205886.3	NP_001192815.2	alpha-mannosidase 2
NM_001205886.1	NP_001192815.1	alpha-mannosidase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2A1	XP_517864.2
Canis lupus familiaris (dog)	MAN2A1	XP_545995.3
Rattus norvegicus (Norway rat)	Man2a1	NP_037111.2
Xenopus tropicalis (tropical clawed frog)	man2a1	NP_001163963.1
Homo sapiens (human)	MAN2A1	NP_002363.2
Gallus gallus (chicken)	MAN2A1	XP_003643094.1
	MAN2A1	XP_002804520.1
Bos taurus (cattle)	MAN2A1	NP_001192815.1
Mus musculus (house mouse)	Man2a1	NP_032575.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAN2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236047	XM_024994256.1 Latest version!	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa239243	NM_001205886.3 Latest version!	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa236046	XM_024994255.1 Latest version!	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa01592	NM_001205886.2 Latest version!	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa01592	NM_001205886.1	Bos taurus mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2A1 ( XM_024994256.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024850024.1 Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236047
Clone ID Related Accession (Same CDS sequence)	XM_024994256.1
Accession Version	XM_024994256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alpha-mannosidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAGA TAAGAGGTTG GTTGAAGACC TTTGATGACT ACTTTAGAGA GAAGACTCAG 
TATATTTTTA ATAATATGGT TGTCAAGTTG AAAGAAGACC CACGGAGAAA ATTTATATGG 
TCTGAGATCT CTTACCTTTC GAAGTGGTGG GAAGCTATAG ATGTTCAGAA GAAGGATGCT 
GTTAAAAGTT TGCTAGAAAA CGGTCAGTTT GAAATTGTGA CAGGTGGCTG GGTAATGCCT 
GATGAAGCTG GTGCACATTA TTTTGCCATA ATTGACCAAC TAATTGAAGG ACATCAGTGG 
CTGGAGAAGA ATCTAGGAGT GAAACCTCGG TCTGGTTGGG CTATCGACCC ATTTGGACAT 
TCACCAACAA TGGCGTATCT TCTAAAACGT GCTGGATTTT CTCACATGCT TATTCAGAGA 
GTTCATTATG CAGTTAAAAA ACACTTTGCA TTGCATAAAA CACTGGAGTT TTTTTGGAGA 
CAAAATTGGG ATCTGGGATC TGTCACAGAC ATTTTTTGCC ATATGATGCC CTTCTACAGC 
TACGACATCC CCCACACTTG TGGGCCTGAT CCTAAAATAT GCTGCCAGTT TGATTTTAAA 
CGGCTTCCTG GAGGCAGATA CGGTTGTCCC TGGGGAGTCC CCCCAGAAAC AATATATCCT 
GGAAATCTCC AGAACAGGGC CGAGATGCTC CTGGACCAGT ACCGAAAGAA GTCAGAGCTC 
TTCCGTACCA CAGTGGTCCT GGCCCCGCTG GGAGACGATT TCCGCTACAC CGAACGCACC 
GAATGGGATC ACCAGTTCAA GAATTACCAG CTGCTTTTTG ATTATATGAA CTCTCATTCT 
CAGTATAATG TTAAGATACA GTTTGGGACT TTATCAGATT ATTTTGATGC ACTAGAAAAA 
GAAGCTTCAG CCAGTAGTAG GAATAGCCAA TCAATATTCC CTGTTGTAAG TGGAGATTTT 
TTCACTTATG CTGACCGAGA TGATCATTAC TGGAGTGGCT ATTTTACATC CAGGCCCTTT 
TACAAACGAA TGGATAGGAT ATTGGAATCC CATTTAAGGG CTGCTGAAAT TCTTTACTAT 
TTCGCCATGA AACAAGCTAA GCAGTACAAG ATAAGTAAAT TTCTTTCATC ATCCCATTAT 
GTGATGCTGA CAGAAGCAAG ACGAAATTTG GGACTATTTC AACATCATGA TGCTATCACA 
GGAACTGCAA AAGATTGGGT GGTTGTGGAT TATGGTACCA GACTCTTTCA TTCACTGATG 
AATTTAAAGA AGATAATTGG ACATTCTGCA CTGCTGCTTA TTTTGAAGGA CAAACACTCA 
TACGACTCTT ATTCTTCGGA TACCTTCCTA GAAATGGATT TGAAACAAAG AACGCAAAGT 
TCTCTACCAC AAAAAAATGT AATAAGTCTG AGCGCAGAGC CAAGGTATCT CGTGGTCTAT 
AACCCTTCAG AACAAGCTCG AAGCTCTGTA GTCTCAGTCT GTGTGAGCTC ATCCGCAGCA 
CAAGTGTCCT CTGCTTCAGG AAAGCCTGTG GAAATCCAAA TGAGTGCAGT GTGGGACACA 
GCAACAACCG TCTCACAGAC AGCCTACGAG ATATCTTTTC TAGCAGAGAT GCCACCACTG 
GGACTGAAAG TGTATAAGAT TTTGGAAACA TCAAGTTCAA ATCCACATTT AGCCAAATAT 
GACCTATATA ATGGTAATAT AGCAAATGAA GGAATTTTTA ACATGAATAA TATAAAAAGT 
GCTCAAGAAG CTATAACTCT AGAGAACTCC TTTATTAGGC TGCGCTTTGG TCAATCTGGG 
CTTATGGAGG AAATGCTAAA TAGAGGAGAT GGTAAGAGTC TTGAGGTGAC AGTACAGTTT 
TCCTGGTATG GAACCACAAG TAAAAGGGAT AAAAGTGGTG CCTACCTCTT CTTACCCGAC 
GGGGAGGCCA AGCCTTACGT TTACACAACA CCACCCTTTG TCAGAGTGCA GCGTGGAAGG 
TTTTATTCAG ACGTGACTTG CTTTTTTGAA CATGTTACCC ACAGAGTTCG ACTGTACAAC 
ATACATGGAG TAGAAGGACA GTCTGTGGAG ATTTCCAATA TTGTAGACAT CCGAAAAGAG 
CATAACTATG AGATTGCAAT GAGAATTTCT TCCAGCATCA ACAGCCAAAA CAGATTTTAT 
ACTGACCTAA ATGGATACCA GATTCAGCCT AGAGTGACGA TGAGCAAATT GCCTCTCCAG 
GCGAACGTCT ACCCCATGAC TACGATGGCG TACATTCAGG ACGCCGAGCG CCGCTTGACT 
CTGCTCTCGG CTCAGTCTTT GGGTGTTTCG AGTCTGAAGA GCGGTCAGAT TGAAGTTATC 
ATGGATCGAA GACTCATGCA AGATGATAAT CGTGGCCTTG AGCAAGGTGT CCATGATAAC 
AAGATTACAG CTAATTTATT TCGAATACTA CTAGAAAAGA GAAGTGTCGT GAATATGGAA 
GAAGACCAGA AGGCAGTCAG TTACCCTTCC CTCCTTAGCC ACATAACGTC TTCTTTCCTG 
AATCACCCTG TCTTCCCAAT GACAGAAAAG GTCCCCGTGC CCACCCTTCA GCTGCTGGGT 
GAATTCTCTC CCTTGATGGC ACCCTTGCCC TGTGACATTC ACCTGGTTAA TCTGAGGACA 
GTGCAGTCGA AGGTGGATGG CAAGCATTCC AACGAGGCCG CCTTGATCCT CCACAGGAAG 
GGGTTTGACT GCCGCTTCTC TAGCAGAGAC ACCGGGCTGC TGTGTTCCAC AACCCAGGGA 
AAGATCCTGG TCCAGAAGCT CTTCAGCAAG TTCACTGTTG TGAGCATCAC ACCTTCGTCA 
TTATCCCTGA TGCATCCCCC TCCAGACGCC CGAAATATAA GTGAGATCAA CTTGAGTCCC 
ATGGAAATCA GCACATTCCG AATCCACTTG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850024.1
CDS	16..2931
Translation	MPQIRGWLKTFDDYFREKTQYIFNNMVVKLKEDPRRKFIWSEISYLSKWWEAIDVQKKDAVKSLLENGQFEIVTGGWVMPDEAGAHYFAIIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDIFCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPETIYPGNLQNRAEMLLDQYRKKSELFRTTVVLAPLGDDFRYTERTEWDHQFKNYQLLFDYMNSHSQYNVKIQFGTLSDYFDALEKEASASSRNSQSIFPVVSGDFFTYADRDDHYWSGYFTSRPFYKRMDRILESHLRAAEILYYFAMKQAKQYKISKFLSSSHYVMLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMNLKKIIGHSALLLILKDKHSYDSYSSDTFLEMDLKQRTQSSLPQKNVISLSAEPRYLVVYNPSEQARSSVVSVCVSSSAAQVSSASGKPVEIQMSAVWDTATTVSQTAYEISFLAEMPPLGLKVYKILETSSSNPHLAKYDLYNGNIANEGIFNMNNIKSAQEAITLENSFIRLRFGQSGLMEEMLNRGDGKSLEVTVQFSWYGTTSKRDKSGAYLFLPDGEAKPYVYTTPPFVRVQRGRFYSDVTCFFEHVTHRVRLYNIHGVEGQSVEISNIVDIRKEHNYEIAMRISSSINSQNRFYTDLNGYQIQPRVTMSKLPLQANVYPMTTMAYIQDAERRLTLLSAQSLGVSSLKSGQIEVIMDRRLMQDDNRGLEQGVHDNKITANLFRILLEKRSVVNMEEDQKAVSYPSLLSHITSSFLNHPVFPMTEKVPVPTLQLLGEFSPLMAPLPCDIHLVNLRTVQSKVDGKHSNEAALILHRKGFDCRFSSRDTGLLCSTTQGKILVQKLFSKFTVVSITPSSLSLMHPPPDARNISEINLSPMEISTFRIHLR

Target ORF information:

RefSeq Version	XM_024994256.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994256.1

ORF Insert Sequence:

ATGCCGCAGA TAAGAGGTTG GTTGAAGACC TTTGATGACT ACTTTAGAGA GAAGACTCAG 
TATATTTTTA ATAATATGGT TGTCAAGTTG AAAGAAGACC CACGGAGAAA ATTTATATGG 
TCTGAGATCT CTTACCTTTC GAAGTGGTGG GAAGCTATAG ATGTTCAGAA GAAGGATGCT 
GTTAAAAGTT TGCTAGAAAA CGGTCAGTTT GAAATTGTGA CAGGTGGCTG GGTAATGCCT 
GATGAAGCTG GTGCACATTA TTTTGCCATA ATTGACCAAC TAATTGAAGG ACATCAGTGG 
CTGGAGAAGA ATCTAGGAGT GAAACCTCGG TCTGGTTGGG CTATCGACCC ATTTGGACAT 
TCACCAACAA TGGCGTATCT TCTAAAACGT GCTGGATTTT CTCACATGCT TATTCAGAGA 
GTTCATTATG CAGTTAAAAA ACACTTTGCA TTGCATAAAA CACTGGAGTT TTTTTGGAGA 
CAAAATTGGG ATCTGGGATC TGTCACAGAC ATTTTTTGCC ATATGATGCC CTTCTACAGC 
TACGACATCC CCCACACTTG TGGGCCTGAT CCTAAAATAT GCTGCCAGTT TGATTTTAAA 
CGGCTTCCTG GAGGCAGATA CGGTTGTCCC TGGGGAGTCC CCCCAGAAAC AATATATCCT 
GGAAATCTCC AGAACAGGGC CGAGATGCTC CTGGACCAGT ACCGAAAGAA GTCAGAGCTC 
TTCCGTACCA CAGTGGTCCT GGCCCCGCTG GGAGACGATT TCCGCTACAC CGAACGCACC 
GAATGGGATC ACCAGTTCAA GAATTACCAG CTGCTTTTTG ATTATATGAA CTCTCATTCT 
CAGTATAATG TTAAGATACA GTTTGGGACT TTATCAGATT ATTTTGATGC ACTAGAAAAA 
GAAGCTTCAG CCAGTAGTAG GAATAGCCAA TCAATATTCC CTGTTGTAAG TGGAGATTTT 
TTCACTTATG CTGACCGAGA TGATCATTAC TGGAGTGGCT ATTTTACATC CAGGCCCTTT 
TACAAACGAA TGGATAGGAT ATTGGAATCC CATTTAAGGG CTGCTGAAAT TCTTTACTAT 
TTCGCCATGA AACAAGCTAA GCAGTACAAG ATAAGTAAAT TTCTTTCATC ATCCCATTAT 
GTGATGCTGA CAGAAGCAAG ACGAAATTTG GGACTATTTC AACATCATGA TGCTATCACA 
GGAACTGCAA AAGATTGGGT GGTTGTGGAT TATGGTACCA GACTCTTTCA TTCACTGATG 
AATTTAAAGA AGATAATTGG ACATTCTGCA CTGCTGCTTA TTTTGAAGGA CAAACACTCA 
TACGACTCTT ATTCTTCGGA TACCTTCCTA GAAATGGATT TGAAACAAAG AACGCAAAGT 
TCTCTACCAC AAAAAAATGT AATAAGTCTG AGCGCAGAGC CAAGGTATCT CGTGGTCTAT 
AACCCTTCAG AACAAGCTCG AAGCTCTGTA GTCTCAGTCT GTGTGAGCTC ATCCGCAGCA 
CAAGTGTCCT CTGCTTCAGG AAAGCCTGTG GAAATCCAAA TGAGTGCAGT GTGGGACACA 
GCAACAACCG TCTCACAGAC AGCCTACGAG ATATCTTTTC TAGCAGAGAT GCCACCACTG 
GGACTGAAAG TGTATAAGAT TTTGGAAACA TCAAGTTCAA ATCCACATTT AGCCAAATAT 
GACCTATATA ATGGTAATAT AGCAAATGAA GGAATTTTTA ACATGAATAA TATAAAAAGT 
GCTCAAGAAG CTATAACTCT AGAGAACTCC TTTATTAGGC TGCGCTTTGG TCAATCTGGG 
CTTATGGAGG AAATGCTAAA TAGAGGAGAT GGTAAGAGTC TTGAGGTGAC AGTACAGTTT 
TCCTGGTATG GAACCACAAG TAAAAGGGAT AAAAGTGGTG CCTACCTCTT CTTACCCGAC 
GGGGAGGCCA AGCCTTACGT TTACACAACA CCACCCTTTG TCAGAGTGCA GCGTGGAAGG 
TTTTATTCAG ACGTGACTTG CTTTTTTGAA CATGTTACCC ACAGAGTTCG ACTGTACAAC 
ATACATGGAG TAGAAGGACA GTCTGTGGAG ATTTCCAATA TTGTAGACAT CCGAAAAGAG 
CATAACTATG AGATTGCAAT GAGAATTTCT TCCAGCATCA ACAGCCAAAA CAGATTTTAT 
ACTGACCTAA ATGGATACCA GATTCAGCCT AGAGTGACGA TGAGCAAATT GCCTCTCCAG 
GCGAACGTCT ACCCCATGAC TACGATGGCG TACATTCAGG ACGCCGAGCG CCGCTTGACT 
CTGCTCTCGG CTCAGTCTTT GGGTGTTTCG AGTCTGAAGA GCGGTCAGAT TGAAGTTATC 
ATGGATCGAA GACTCATGCA AGATGATAAT CGTGGCCTTG AGCAAGGTGT CCATGATAAC 
AAGATTACAG CTAATTTATT TCGAATACTA CTAGAAAAGA GAAGTGTCGT GAATATGGAA 
GAAGACCAGA AGGCAGTCAG TTACCCTTCC CTCCTTAGCC ACATAACGTC TTCTTTCCTG 
AATCACCCTG TCTTCCCAAT GACAGAAAAG GTCCCCGTGC CCACCCTTCA GCTGCTGGGT 
GAATTCTCTC CCTTGATGGC ACCCTTGCCC TGTGACATTC ACCTGGTTAA TCTGAGGACA 
GTGCAGTCGA AGGTGGATGG CAAGCATTCC AACGAGGCCG CCTTGATCCT CCACAGGAAG 
GGGTTTGACT GCCGCTTCTC TAGCAGAGAC ACCGGGCTGC TGTGTTCCAC AACCCAGGGA 
AAGATCCTGG TCCAGAAGCT CTTCAGCAAG TTCACTGTTG TGAGCATCAC ACCTTCGTCA 
TTATCCCTGA TGCATCCCCC TCCAGACGCC CGAAATATAA GTGAGATCAA CTTGAGTCCC 
ATGGAAATCA GCACATTCCG AATCCACTTG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A1 ( NM_001205886.3 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192815.2 Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

Price & Availability↑

CloneID	OBa239243
Clone ID Related Accession (Same CDS sequence)	NM_001205886.3
Accession Version	NM_001205886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	alpha-mannosidase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000007.1. On Dec 20, 2018 this sequence version replaced NM_001205886.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.26643.1, SRR5571261.3853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAGAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192815.2
CDS	1..3438
Translation	MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPKSPRREGSFPQGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGTKNSKGNFSHGAGSPLLPSKRLSLTVDPADCLFASQSGSQSSEVQMLDVYSLIPFDNPDGGVWKQGFDITYKPDEWDTKPLQVFVVPHSHNDPGWLKTFDDYFREKTQYIFNNMVVKLKEDPRRKFIWSEISYLSKWWEAIDVQKKDAVKSLLENGQFEIVTGGWVMPDEAGAHYFAIIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDIFCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPETIYPGNLQNRAEMLLDQYRKKSELFRTTVVLAPLGDDFRYTERTEWDHQFKNYQLLFDYMNSHSQYNVKIQFGTLSDYFDALEKEASASSRNSQSIFPVVSGDFFTYADRDDHYWSGYFTSRPFYKRMDRILESHLRAAEILYYFAMKQAKQYKISKFLSSSHYVMLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMNLKKIIGHSALLLILKDKHSYDSYSSDTFLEMDLKQRTQSSLPQKNVISLSAEPRYLVVYNPSEQARSSVVSVCVSSSAAQVSSASGKPVEIQMSAVWDTATTVSQTAYEISFLAEMPPLGLKVYKILETSSSNPHLAKYDLYNGNIANEGIFNMNNIKSAQEAITLENSFIRLRFGQSGLMEEMLNRGDGKSLEVTVQFSWYGTTSKRDKSGAYLFLPDGEAKPYVYTTPPFVRVQRGRFYSDVTCFFEHVTHRVRLYNIHGVEGQSVEISNIVDIRKEHNYEIAMRISSSINSQNRFYTDLNGYQIQPRVTMSKLPLQANVYPMTTMAYIQDAERRLTLLSAQSLGVSSLKSGQIEVIMDRRLMQDDNRGLEQGVHDNKITANLFRILLEKRSVVNMEEDQKAVSYPSLLSHITSSFLNHPVFPMTEKVPVPTLQLLGEFSPLMAPLPCDIHLVNLRTVQSKVDGKHSNEAALILHRKGFDCRFSSRDTGLLCSTTQGKILVQKLFSKFTVVSITPSSLSLMHPPPDARNISEINLSPMEISTFRIHLR

Target ORF information:

RefSeq Version	NM_001205886.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205886.3

ORF Insert Sequence:

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAGAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A1 ( XM_024994255.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024850023.1 Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA.

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CloneID	OBa236046
Clone ID Related Accession (Same CDS sequence)	XM_024994255.1
Accession Version	XM_024994255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alpha-mannosidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGATGTT GGATGTTTAC AGTCTAATTC CTTTTGACAA TCCCGATGGT 
GGAGTTTGGA AGCAAGGATT TGACATCACT TATAAGCCTG ATGAATGGGA CACTAAGCCC 
CTTCAAGTCT TCGTGGTGCC TCACTCCCAT AATGACCCAG GTTGGTTGAA GACCTTTGAT 
GACTACTTTA GAGAGAAGAC TCAGTATATT TTTAATAATA TGGTTGTCAA GTTGAAAGAA 
GACCCACGGA GAAAATTTAT ATGGTCTGAG ATCTCTTACC TTTCGAAGTG GTGGGAAGCT 
ATAGATGTTC AGAAGAAGGA TGCTGTTAAA AGTTTGCTAG AAAACGGTCA GTTTGAAATT 
GTGACAGGTG GCTGGGTAAT GCCTGATGAA GCTGGTGCAC ATTATTTTGC CATAATTGAC 
CAACTAATTG AAGGACATCA GTGGCTGGAG AAGAATCTAG GAGTGAAACC TCGGTCTGGT 
TGGGCTATCG ACCCATTTGG ACATTCACCA ACAATGGCGT ATCTTCTAAA ACGTGCTGGA 
TTTTCTCACA TGCTTATTCA GAGAGTTCAT TATGCAGTTA AAAAACACTT TGCATTGCAT 
AAAACACTGG AGTTTTTTTG GAGACAAAAT TGGGATCTGG GATCTGTCAC AGACATTTTT 
TGCCATATGA TGCCCTTCTA CAGCTACGAC ATCCCCCACA CTTGTGGGCC TGATCCTAAA 
ATATGCTGCC AGTTTGATTT TAAACGGCTT CCTGGAGGCA GATACGGTTG TCCCTGGGGA 
GTCCCCCCAG AAACAATATA TCCTGGAAAT CTCCAGAACA GGGCCGAGAT GCTCCTGGAC 
CAGTACCGAA AGAAGTCAGA GCTCTTCCGT ACCACAGTGG TCCTGGCCCC GCTGGGAGAC 
GATTTCCGCT ACACCGAACG CACCGAATGG GATCACCAGT TCAAGAATTA CCAGCTGCTT 
TTTGATTATA TGAACTCTCA TTCTCAGTAT AATGTTAAGA TACAGTTTGG GACTTTATCA 
GATTATTTTG ATGCACTAGA AAAAGAAGCT TCAGCCAGTA GTAGGAATAG CCAATCAATA 
TTCCCTGTTG TAAGTGGAGA TTTTTTCACT TATGCTGACC GAGATGATCA TTACTGGAGT 
GGCTATTTTA CATCCAGGCC CTTTTACAAA CGAATGGATA GGATATTGGA ATCCCATTTA 
AGGGCTGCTG AAATTCTTTA CTATTTCGCC ATGAAACAAG CTAAGCAGTA CAAGATAAGT 
AAATTTCTTT CATCATCCCA TTATGTGATG CTGACAGAAG CAAGACGAAA TTTGGGACTA 
TTTCAACATC ATGATGCTAT CACAGGAACT GCAAAAGATT GGGTGGTTGT GGATTATGGT 
ACCAGACTCT TTCATTCACT GATGAATTTA AAGAAGATAA TTGGACATTC TGCACTGCTG 
CTTATTTTGA AGGACAAACA CTCATACGAC TCTTATTCTT CGGATACCTT CCTAGAAATG 
GATTTGAAAC AAAGAACGCA AAGTTCTCTA CCACAAAAAA ATGTAATAAG TCTGAGCGCA 
GAGCCAAGGT ATCTCGTGGT CTATAACCCT TCAGAACAAG CTCGAAGCTC TGTAGTCTCA 
GTCTGTGTGA GCTCATCCGC AGCACAAGTG TCCTCTGCTT CAGGAAAGCC TGTGGAAATC 
CAAATGAGTG CAGTGTGGGA CACAGCAACA ACCGTCTCAC AGACAGCCTA CGAGATATCT 
TTTCTAGCAG AGATGCCACC ACTGGGACTG AAAGTGTATA AGATTTTGGA AACATCAAGT 
TCAAATCCAC ATTTAGCCAA ATATGACCTA TATAATGGTA ATATAGCAAA TGAAGGAATT 
TTTAACATGA ATAATATAAA AAGTGCTCAA GAAGCTATAA CTCTAGAGAA CTCCTTTATT 
AGGCTGCGCT TTGGTCAATC TGGGCTTATG GAGGAAATGC TAAATAGAGG AGATGGTAAG 
AGTCTTGAGG TGACAGTACA GTTTTCCTGG TATGGAACCA CAAGTAAAAG GGATAAAAGT 
GGTGCCTACC TCTTCTTACC CGACGGGGAG GCCAAGCCTT ACGTTTACAC AACACCACCC 
TTTGTCAGAG TGCAGCGTGG AAGGTTTTAT TCAGACGTGA CTTGCTTTTT TGAACATGTT 
ACCCACAGAG TTCGACTGTA CAACATACAT GGAGTAGAAG GACAGTCTGT GGAGATTTCC 
AATATTGTAG ACATCCGAAA AGAGCATAAC TATGAGATTG CAATGAGAAT TTCTTCCAGC 
ATCAACAGCC AAAACAGATT TTATACTGAC CTAAATGGAT ACCAGATTCA GCCTAGAGTG 
ACGATGAGCA AATTGCCTCT CCAGGCGAAC GTCTACCCCA TGACTACGAT GGCGTACATT 
CAGGACGCCG AGCGCCGCTT GACTCTGCTC TCGGCTCAGT CTTTGGGTGT TTCGAGTCTG 
AAGAGCGGTC AGATTGAAGT TATCATGGAT CGAAGACTCA TGCAAGATGA TAATCGTGGC 
CTTGAGCAAG GTGTCCATGA TAACAAGATT ACAGCTAATT TATTTCGAAT ACTACTAGAA 
AAGAGAAGTG TCGTGAATAT GGAAGAAGAC CAGAAGGCAG TCAGTTACCC TTCCCTCCTT 
AGCCACATAA CGTCTTCTTT CCTGAATCAC CCTGTCTTCC CAATGACAGA AAAGGTCCCC 
GTGCCCACCC TTCAGCTGCT GGGTGAATTC TCTCCCTTGA TGGCACCCTT GCCCTGTGAC 
ATTCACCTGG TTAATCTGAG GACAGTGCAG TCGAAGGTGG ATGGCAAGCA TTCCAACGAG 
GCCGCCTTGA TCCTCCACAG GAAGGGGTTT GACTGCCGCT TCTCTAGCAG AGACACCGGG 
CTGCTGTGTT CCACAACCCA GGGAAAGATC CTGGTCCAGA AGCTCTTCAG CAAGTTCACT 
GTTGTGAGCA TCACACCTTC GTCATTATCC CTGATGCATC CCCCTCCAGA CGCCCGAAAT 
ATAAGTGAGA TCAACTTGAG TCCCATGGAA ATCAGCACAT TCCGAATCCA CTTGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850023.1
CDS	167..3346
Translation	MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPKSPRREGSFPQMLDVYSLIPFDNPDGGVWKQGFDITYKPDEWDTKPLQVFVVPHSHNDPGWLKTFDDYFREKTQYIFNNMVVKLKEDPRRKFIWSEISYLSKWWEAIDVQKKDAVKSLLENGQFEIVTGGWVMPDEAGAHYFAIIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDIFCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPETIYPGNLQNRAEMLLDQYRKKSELFRTTVVLAPLGDDFRYTERTEWDHQFKNYQLLFDYMNSHSQYNVKIQFGTLSDYFDALEKEASASSRNSQSIFPVVSGDFFTYADRDDHYWSGYFTSRPFYKRMDRILESHLRAAEILYYFAMKQAKQYKISKFLSSSHYVMLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMNLKKIIGHSALLLILKDKHSYDSYSSDTFLEMDLKQRTQSSLPQKNVISLSAEPRYLVVYNPSEQARSSVVSVCVSSSAAQVSSASGKPVEIQMSAVWDTATTVSQTAYEISFLAEMPPLGLKVYKILETSSSNPHLAKYDLYNGNIANEGIFNMNNIKSAQEAITLENSFIRLRFGQSGLMEEMLNRGDGKSLEVTVQFSWYGTTSKRDKSGAYLFLPDGEAKPYVYTTPPFVRVQRGRFYSDVTCFFEHVTHRVRLYNIHGVEGQSVEISNIVDIRKEHNYEIAMRISSSINSQNRFYTDLNGYQIQPRVTMSKLPLQANVYPMTTMAYIQDAERRLTLLSAQSLGVSSLKSGQIEVIMDRRLMQDDNRGLEQGVHDNKITANLFRILLEKRSVVNMEEDQKAVSYPSLLSHITSSFLNHPVFPMTEKVPVPTLQLLGEFSPLMAPLPCDIHLVNLRTVQSKVDGKHSNEAALILHRKGFDCRFSSRDTGLLCSTTQGKILVQKLFSKFTVVSITPSSLSLMHPPPDARNISEINLSPMEISTFRIHLR

Target ORF information:

RefSeq Version	XM_024994255.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994255.1

ORF Insert Sequence:

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGATGTT GGATGTTTAC AGTCTAATTC CTTTTGACAA TCCCGATGGT 
GGAGTTTGGA AGCAAGGATT TGACATCACT TATAAGCCTG ATGAATGGGA CACTAAGCCC 
CTTCAAGTCT TCGTGGTGCC TCACTCCCAT AATGACCCAG GTTGGTTGAA GACCTTTGAT 
GACTACTTTA GAGAGAAGAC TCAGTATATT TTTAATAATA TGGTTGTCAA GTTGAAAGAA 
GACCCACGGA GAAAATTTAT ATGGTCTGAG ATCTCTTACC TTTCGAAGTG GTGGGAAGCT 
ATAGATGTTC AGAAGAAGGA TGCTGTTAAA AGTTTGCTAG AAAACGGTCA GTTTGAAATT 
GTGACAGGTG GCTGGGTAAT GCCTGATGAA GCTGGTGCAC ATTATTTTGC CATAATTGAC 
CAACTAATTG AAGGACATCA GTGGCTGGAG AAGAATCTAG GAGTGAAACC TCGGTCTGGT 
TGGGCTATCG ACCCATTTGG ACATTCACCA ACAATGGCGT ATCTTCTAAA ACGTGCTGGA 
TTTTCTCACA TGCTTATTCA GAGAGTTCAT TATGCAGTTA AAAAACACTT TGCATTGCAT 
AAAACACTGG AGTTTTTTTG GAGACAAAAT TGGGATCTGG GATCTGTCAC AGACATTTTT 
TGCCATATGA TGCCCTTCTA CAGCTACGAC ATCCCCCACA CTTGTGGGCC TGATCCTAAA 
ATATGCTGCC AGTTTGATTT TAAACGGCTT CCTGGAGGCA GATACGGTTG TCCCTGGGGA 
GTCCCCCCAG AAACAATATA TCCTGGAAAT CTCCAGAACA GGGCCGAGAT GCTCCTGGAC 
CAGTACCGAA AGAAGTCAGA GCTCTTCCGT ACCACAGTGG TCCTGGCCCC GCTGGGAGAC 
GATTTCCGCT ACACCGAACG CACCGAATGG GATCACCAGT TCAAGAATTA CCAGCTGCTT 
TTTGATTATA TGAACTCTCA TTCTCAGTAT AATGTTAAGA TACAGTTTGG GACTTTATCA 
GATTATTTTG ATGCACTAGA AAAAGAAGCT TCAGCCAGTA GTAGGAATAG CCAATCAATA 
TTCCCTGTTG TAAGTGGAGA TTTTTTCACT TATGCTGACC GAGATGATCA TTACTGGAGT 
GGCTATTTTA CATCCAGGCC CTTTTACAAA CGAATGGATA GGATATTGGA ATCCCATTTA 
AGGGCTGCTG AAATTCTTTA CTATTTCGCC ATGAAACAAG CTAAGCAGTA CAAGATAAGT 
AAATTTCTTT CATCATCCCA TTATGTGATG CTGACAGAAG CAAGACGAAA TTTGGGACTA 
TTTCAACATC ATGATGCTAT CACAGGAACT GCAAAAGATT GGGTGGTTGT GGATTATGGT 
ACCAGACTCT TTCATTCACT GATGAATTTA AAGAAGATAA TTGGACATTC TGCACTGCTG 
CTTATTTTGA AGGACAAACA CTCATACGAC TCTTATTCTT CGGATACCTT CCTAGAAATG 
GATTTGAAAC AAAGAACGCA AAGTTCTCTA CCACAAAAAA ATGTAATAAG TCTGAGCGCA 
GAGCCAAGGT ATCTCGTGGT CTATAACCCT TCAGAACAAG CTCGAAGCTC TGTAGTCTCA 
GTCTGTGTGA GCTCATCCGC AGCACAAGTG TCCTCTGCTT CAGGAAAGCC TGTGGAAATC 
CAAATGAGTG CAGTGTGGGA CACAGCAACA ACCGTCTCAC AGACAGCCTA CGAGATATCT 
TTTCTAGCAG AGATGCCACC ACTGGGACTG AAAGTGTATA AGATTTTGGA AACATCAAGT 
TCAAATCCAC ATTTAGCCAA ATATGACCTA TATAATGGTA ATATAGCAAA TGAAGGAATT 
TTTAACATGA ATAATATAAA AAGTGCTCAA GAAGCTATAA CTCTAGAGAA CTCCTTTATT 
AGGCTGCGCT TTGGTCAATC TGGGCTTATG GAGGAAATGC TAAATAGAGG AGATGGTAAG 
AGTCTTGAGG TGACAGTACA GTTTTCCTGG TATGGAACCA CAAGTAAAAG GGATAAAAGT 
GGTGCCTACC TCTTCTTACC CGACGGGGAG GCCAAGCCTT ACGTTTACAC AACACCACCC 
TTTGTCAGAG TGCAGCGTGG AAGGTTTTAT TCAGACGTGA CTTGCTTTTT TGAACATGTT 
ACCCACAGAG TTCGACTGTA CAACATACAT GGAGTAGAAG GACAGTCTGT GGAGATTTCC 
AATATTGTAG ACATCCGAAA AGAGCATAAC TATGAGATTG CAATGAGAAT TTCTTCCAGC 
ATCAACAGCC AAAACAGATT TTATACTGAC CTAAATGGAT ACCAGATTCA GCCTAGAGTG 
ACGATGAGCA AATTGCCTCT CCAGGCGAAC GTCTACCCCA TGACTACGAT GGCGTACATT 
CAGGACGCCG AGCGCCGCTT GACTCTGCTC TCGGCTCAGT CTTTGGGTGT TTCGAGTCTG 
AAGAGCGGTC AGATTGAAGT TATCATGGAT CGAAGACTCA TGCAAGATGA TAATCGTGGC 
CTTGAGCAAG GTGTCCATGA TAACAAGATT ACAGCTAATT TATTTCGAAT ACTACTAGAA 
AAGAGAAGTG TCGTGAATAT GGAAGAAGAC CAGAAGGCAG TCAGTTACCC TTCCCTCCTT 
AGCCACATAA CGTCTTCTTT CCTGAATCAC CCTGTCTTCC CAATGACAGA AAAGGTCCCC 
GTGCCCACCC TTCAGCTGCT GGGTGAATTC TCTCCCTTGA TGGCACCCTT GCCCTGTGAC 
ATTCACCTGG TTAATCTGAG GACAGTGCAG TCGAAGGTGG ATGGCAAGCA TTCCAACGAG 
GCCGCCTTGA TCCTCCACAG GAAGGGGTTT GACTGCCGCT TCTCTAGCAG AGACACCGGG 
CTGCTGTGTT CCACAACCCA GGGAAAGATC CTGGTCCAGA AGCTCTTCAG CAAGTTCACT 
GTTGTGAGCA TCACACCTTC GTCATTATCC CTGATGCATC CCCCTCCAGA CGCCCGAAAT 
ATAAGTGAGA TCAACTTGAG TCCCATGGAA ATCAGCACAT TCCGAATCCA CTTGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A1 ( NM_001205886.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192815.1 Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

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CloneID	OBa01592
Clone ID Related Accession (Same CDS sequence)	NM_001205886.2 , NM_001205886.1
Accession Version	NM_001205886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	alpha-mannosidase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02021699.1, CB452086.1 and HX937636.1. On Aug 24, 2017 this sequence version replaced NM_001205886.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403641 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192815.1
CDS	1..3438
Translation	MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPKSPRREGSFPQGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGTKNSKGNFSHGAGSPLLPSKRLSLTVDPADCLFASQSGSQSSEVQMLDVYSLIPFDNPDGGVWKQGFDITYKPDEWDTKPLQVFVVPHSHNDPGWLKTFDDYFREKTQYIFNNMVVKLKEDPRRKFIWSEISYLSKWWEAIDVQKKDAVKSLLENGQFEIVTGGWVMPDEAGAHYFAIIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDIFCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPETIYPGNLQNRAEMLLDQYRKKSELFRTTVVLAPLGDDFRYTERTEWDHQFKNYQLLFDYMNSHSQYNVKIQFGTLSDYFDALEKEASASSRNSQSIFPVVSGDFFTYADRDDHYWSGYFTSRPFYKRMDRILESHLRAAEILYYFAMKQAKQYKISKFLSSSHYVMLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMNLKKIIGHSALLLILKDKHSYDSYSSDTFLEMDLKQRTQSSLPQKNVISLSAEPRYLVVYNPSEQARSSVVSVCVSSSAAQVSSASGKPVEIQMSAVWDTATTVSQTAYEISFLAEMPPLGLKVYKILETSSSNPHLAKYDLYNGNIANEGIFNMNNIKSAQEAITLENSFIRLRFGQSGLMEEMLNKGDGKSLEVTVQFSWYGTTSKRDKSGAYLFLPDGEAKPYVYTTPPFVRVQRGRFYSDVTCFFEHVTHRVRLYNIHGVEGQSVEISNIVDIRKEHNYEIAMRISSSINSQNRFYTDLNGYQIQPRVTMSKLPLQANVYPMTTMAYIQDAERRLTLLSAQSLGVSSLKSGQIEVIMDRRLMQDDNRGLEQGVHDNKITANLFRILLEKRSVVNMEEDQKAVSYPSLLSHITSSFLNHPVFPMTEKVPVPTLQLLGEFSPLMAPLPCDIHLVNLRTVQSKVDGKHSNEAALILHRKGFDCRFSSRDTGLLCSTTQGKILVQKLFSKFTVVSITPSSLSLMHPPPDARNISEINLSPMEISTFRIHLR

Target ORF information:

RefSeq Version	NM_001205886.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205886.2

ORF Insert Sequence:

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A1 ( NM_001205886.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192815.1 Bos taurus mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA.

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CloneID	OBa01592
Clone ID Related Accession (Same CDS sequence)	NM_001205886.2 , NM_001205886.1
Accession Version	NM_001205886.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-04-23
Organism	Bos taurus(Cattle)
Product	alpha-mannosidase 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03039677.1, AAFC03039678.1, AAFC03057257.1 and AAFC03057258.1. On Apr 20, 2011 this sequence version replaced XM_599803.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192815.1
CDS	1..3438
Translation	MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPKSPRREGSFPQGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGTKNSKGNFSHGAGSPLLPSKRLSLTVDPADCLFASQSGSQSSEVQMLDVYSLIPFDNPDGGVWKQGFDITYKPDEWDTKPLQVFVVPHSHNDPGWLKTFDDYFREKTQYIFNNMVVKLKEDPRRKFIWSEISYLSKWWEAIDVQKKDAVKSLLENGQFEIVTGGWVMPDEAGAHYFAIIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDIFCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPETIYPGNLQNRAEMLLDQYRKKSELFRTTVVLAPLGDDFRYTERTEWDHQFKNYQLLFDYMNSHSQYNVKIQFGTLSDYFDALEKEASASSRNSQSIFPVVSGDFFTYADRDDHYWSGYFTSRPFYKRMDRILESHLRAAEILYYFAMKQAKQYKISKFLSSSHYVMLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMNLKKIIGHSALLLILKDKHSYDSYSSDTFLEMDLKQRTQSSLPQKNVISLSAEPRYLVVYNPSEQARSSVVSVCVSSSAAQVSSASGKPVEIQMSAVWDTATTVSQTAYEISFLAEMPPLGLKVYKILETSSSNPHLAKYDLYNGNIANEGIFNMNNIKSAQEAITLENSFIRLRFGQSGLMEEMLNKGDGKSLEVTVQFSWYGTTSKRDKSGAYLFLPDGEAKPYVYTTPPFVRVQRGRFYSDVTCFFEHVTHRVRLYNIHGVEGQSVEISNIVDIRKEHNYEIAMRISSSINSQNRFYTDLNGYQIQPRVTMSKLPLQANVYPMTTMAYIQDAERRLTLLSAQSLGVSSLKSGQIEVIMDRRLMQDDNRGLEQGVHDNKITANLFRILLEKRSVVNMEEDQKAVSYPSLLSHITSSFLNHPVFPMTEKVPVPTLQLLGEFSPLMAPLPCDIHLVNLRTVQSKVDGKHSNEAALILHRKGFDCRFSSRDTGLLCSTTQGKILVQKLFSKFTVVSITPSSLSLMHPPPDARNISEINLSPMEISTFRIHLR

Target ORF information:

RefSeq Version	NM_001205886.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205886.1

ORF Insert Sequence:

ATGAAGTTGA GCCGGCAGTT CACCGTTTTC GGCAGCGCTA TCTTCTGCGT GGTGATCTTC 
TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTGGACTACC CCAAGAGCCC GCGCCGCGAG 
GGCTCCTTCC CGCAGGGTCA GCTTTCAATG TTGCAAGAAA AAATAGACCA TTTGGAACGC 
TTGCTAGCTG AGAATAATGA AATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT 
GAATCTGTGG AGGATGGTAC GAAAAACTCA AAAGGCAATT TCAGCCATGG TGCTGGCTCT 
CCTCTTCTGC CATCGAAACG TTTGTCCCTC ACAGTTGACC CTGCAGACTG TCTGTTTGCT 
TCACAAAGTG GAAGTCAGAG TTCAGAGGTG CAGATGTTGG ATGTTTACAG TCTAATTCCT 
TTTGACAATC CCGATGGTGG AGTTTGGAAG CAAGGATTTG ACATCACTTA TAAGCCTGAT 
GAATGGGACA CTAAGCCCCT TCAAGTCTTC GTGGTGCCTC ACTCCCATAA TGACCCAGGT 
TGGTTGAAGA CCTTTGATGA CTACTTTAGA GAGAAGACTC AGTATATTTT TAATAATATG 
GTTGTCAAGT TGAAAGAAGA CCCACGGAGA AAATTTATAT GGTCTGAGAT CTCTTACCTT 
TCGAAGTGGT GGGAAGCTAT AGATGTTCAG AAGAAGGATG CTGTTAAAAG TTTGCTAGAA 
AACGGTCAGT TTGAAATTGT GACAGGTGGC TGGGTAATGC CTGATGAAGC TGGTGCACAT 
TATTTTGCCA TAATTGACCA ACTAATTGAA GGACATCAGT GGCTGGAGAA GAATCTAGGA 
GTGAAACCTC GGTCTGGTTG GGCTATCGAC CCATTTGGAC ATTCACCAAC AATGGCGTAT 
CTTCTAAAAC GTGCTGGATT TTCTCACATG CTTATTCAGA GAGTTCATTA TGCAGTTAAA 
AAACACTTTG CATTGCATAA AACACTGGAG TTTTTTTGGA GACAAAATTG GGATCTGGGA 
TCTGTCACAG ACATTTTTTG CCATATGATG CCCTTCTACA GCTACGACAT CCCCCACACT 
TGTGGGCCTG ATCCTAAAAT ATGCTGCCAG TTTGATTTTA AACGGCTTCC TGGAGGCAGA 
TACGGTTGTC CCTGGGGAGT CCCCCCAGAA ACAATATATC CTGGAAATCT CCAGAACAGG 
GCCGAGATGC TCCTGGACCA GTACCGAAAG AAGTCAGAGC TCTTCCGTAC CACAGTGGTC 
CTGGCCCCGC TGGGAGACGA TTTCCGCTAC ACCGAACGCA CCGAATGGGA TCACCAGTTC 
AAGAATTACC AGCTGCTTTT TGATTATATG AACTCTCATT CTCAGTATAA TGTTAAGATA 
CAGTTTGGGA CTTTATCAGA TTATTTTGAT GCACTAGAAA AAGAAGCTTC AGCCAGTAGT 
AGGAATAGCC AATCAATATT CCCTGTTGTA AGTGGAGATT TTTTCACTTA TGCTGACCGA 
GATGATCATT ACTGGAGTGG CTATTTTACA TCCAGGCCCT TTTACAAACG AATGGATAGG 
ATATTGGAAT CCCATTTAAG GGCTGCTGAA ATTCTTTACT ATTTCGCCAT GAAACAAGCT 
AAGCAGTACA AGATAAGTAA ATTTCTTTCA TCATCCCATT ATGTGATGCT GACAGAAGCA 
AGACGAAATT TGGGACTATT TCAACATCAT GATGCTATCA CAGGAACTGC AAAAGATTGG 
GTGGTTGTGG ATTATGGTAC CAGACTCTTT CATTCACTGA TGAATTTAAA GAAGATAATT 
GGACATTCTG CACTGCTGCT TATTTTGAAG GACAAACACT CATACGACTC TTATTCTTCG 
GATACCTTCC TAGAAATGGA TTTGAAACAA AGAACGCAAA GTTCTCTACC ACAAAAAAAT 
GTAATAAGTC TGAGCGCAGA GCCAAGGTAT CTCGTGGTCT ATAACCCTTC AGAACAAGCT 
CGAAGCTCTG TAGTCTCAGT CTGTGTGAGC TCATCCGCAG CACAAGTGTC CTCTGCTTCA 
GGAAAGCCTG TGGAAATCCA AATGAGTGCA GTGTGGGACA CAGCAACAAC CGTCTCACAG 
ACAGCCTACG AGATATCTTT TCTAGCAGAG ATGCCACCAC TGGGACTGAA AGTGTATAAG 
ATTTTGGAAA CATCAAGTTC AAATCCACAT TTAGCCAAAT ATGACCTATA TAATGGTAAT 
ATAGCAAATG AAGGAATTTT TAACATGAAT AATATAAAAA GTGCTCAAGA AGCTATAACT 
CTAGAGAACT CCTTTATTAG GCTGCGCTTT GGTCAATCTG GGCTTATGGA GGAAATGCTA 
AATAAAGGAG ATGGTAAGAG TCTTGAGGTG ACAGTACAGT TTTCCTGGTA TGGAACCACA 
AGTAAAAGGG ATAAAAGTGG TGCCTACCTC TTCTTACCCG ACGGGGAGGC CAAGCCTTAC 
GTTTACACAA CACCACCCTT TGTCAGAGTG CAGCGTGGAA GGTTTTATTC AGACGTGACT 
TGCTTTTTTG AACATGTTAC CCACAGAGTT CGACTGTACA ACATACATGG AGTAGAAGGA 
CAGTCTGTGG AGATTTCCAA TATTGTAGAC ATCCGAAAAG AGCATAACTA TGAGATTGCA 
ATGAGAATTT CTTCCAGCAT CAACAGCCAA AACAGATTTT ATACTGACCT AAATGGATAC 
CAGATTCAGC CTAGAGTGAC GATGAGCAAA TTGCCTCTCC AGGCGAACGT CTACCCCATG 
ACTACGATGG CGTACATTCA GGACGCCGAG CGCCGCTTGA CTCTGCTCTC GGCTCAGTCT 
TTGGGTGTTT CGAGTCTGAA GAGCGGTCAG ATTGAAGTTA TCATGGATCG AAGACTCATG 
CAAGATGATA ATCGTGGCCT TGAGCAAGGT GTCCATGATA ACAAGATTAC AGCTAATTTA 
TTTCGAATAC TACTAGAAAA GAGAAGTGTC GTGAATATGG AAGAAGACCA GAAGGCAGTC 
AGTTACCCTT CCCTCCTTAG CCACATAACG TCTTCTTTCC TGAATCACCC TGTCTTCCCA 
ATGACAGAAA AGGTCCCCGT GCCCACCCTT CAGCTGCTGG GTGAATTCTC TCCCTTGATG 
GCACCCTTGC CCTGTGACAT TCACCTGGTT AATCTGAGGA CAGTGCAGTC GAAGGTGGAT 
GGCAAGCATT CCAACGAGGC CGCCTTGATC CTCCACAGGA AGGGGTTTGA CTGCCGCTTC 
TCTAGCAGAG ACACCGGGCT GCTGTGTTCC ACAACCCAGG GAAAGATCCT GGTCCAGAAG 
CTCTTCAGCA AGTTCACTGT TGTGAGCATC ACACCTTCGT CATTATCCCT GATGCATCCC 
CCTCCAGACG CCCGAAATAT AAGTGAGATC AACTTGAGTC CCATGGAAAT CAGCACATTC 
CGAATCCACT TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MAN2A1 cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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