COL22A1 cDNA ORF clone, Bos taurus(cattle)

The following COL22A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL22A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222589 XM_025002008.1
Latest version!
Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222590 XM_015474404.2
Latest version!
Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa25838 XM_002692600.4
Latest version!
Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa25838 XM_010822664.2
Latest version!
Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa50351 XM_002692600.5
Latest version!
Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92484 XM_015474404.1
Latest version!
Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92484 XM_015466293.1
Latest version!
Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222589
    Clone ID Related Accession (Same CDS sequence) XM_025002008.1
    Accession Version XM_025002008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGCCTTGC TGCTGCTCGA CCTGGTAACG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857776.1
    CDS496..5178
    Translation

    Target ORF information:

    RefSeq Version XM_025002008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025002008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGCCTTGC TGCTGCTCGA CCTGGTAACG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222590
    Clone ID Related Accession (Same CDS sequence) XM_015474404.2
    Accession Version XM_015474404.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329890.2
    CDS496..5100
    Translation

    Target ORF information:

    RefSeq Version XM_015474404.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474404.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25838
    Clone ID Related Accession (Same CDS sequence) XM_002692600.4 , XM_010822664.2
    Accession Version XM_002692600.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692600.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692646.2
    CDS497..5341
    Translation

    Target ORF information:

    RefSeq Version XM_002692600.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692600.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25838
    Clone ID Related Accession (Same CDS sequence) XM_002692600.4 , XM_010822664.2
    Accession Version XM_010822664.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820966.2
    CDS497..5341
    Translation

    Target ORF information:

    RefSeq Version XM_010822664.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822664.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50351
    Clone ID Related Accession (Same CDS sequence) XM_002692600.5
    Accession Version XM_002692600.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002692600.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692646.3
    CDS496..5340
    Translation

    Target ORF information:

    RefSeq Version XM_002692600.5
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692600.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC
    CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT
    AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC
    GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC
    GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92484
    Clone ID Related Accession (Same CDS sequence) XM_015474404.1 , XM_015466293.1
    Accession Version XM_015474404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329890.1
    CDS497..5101
    Translation

    Target ORF information:

    RefSeq Version XM_015474404.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92484
    Clone ID Related Accession (Same CDS sequence) XM_015474404.1 , XM_015466293.1
    Accession Version XM_015466293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-1(XXII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321779.1
    CDS497..5101
    Translation

    Target ORF information:

    RefSeq Version XM_015466293.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
    CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT
    TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA
    GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC
    CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG
    CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC
    GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA
    CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC
    GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC
    ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG
    CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC
    AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT
    CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG
    TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG
    ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT
    GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT
    ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG
    AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC
    CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG
    CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG
    GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC
    GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA
    CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC
    TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC
    ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA
    CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG
    GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC
    TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA
    GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG
    CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG
    GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG
    GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG
    AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG
    GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA
    CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT
    CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG
    GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG
    CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA
    AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA
    GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG
    GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT
    GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA
    GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT
    ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG
    GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA
    GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA
    GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG
    AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT
    TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC
    CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC
    GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT
    GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC
    AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG
    TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG
    CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT
    GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC
    ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA
    CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT
    GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC
    CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT
    CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT
    GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT
    ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA
    TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC
    GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG
    AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA
    GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC
    GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA
    CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG
    CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG
    AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG
    CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG
    GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC
    CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.