COL22A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL22A1
Entrez Gene ID	521058
Full Name	collagen type XXII alpha 1 chain
General protein information	Preferred Names collagen type XXII alpha 1 chain Names collagen alpha-1(XXII) chain collagen, type XXII, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015474404.1	XP_015329890.1	collagen alpha-1(XXII) chain isoform X2
XM_002692600.5	XP_002692646.3	collagen alpha-1(XXII) chain isoform X1
XM_015466293.1	XP_015321779.1	collagen alpha-1(XXII) chain isoform X2
XM_002692600.4	XP_002692646.2	collagen alpha-1(XXII) chain isoform X1
XM_010822664.2	XP_010820966.2	collagen alpha-1(XXII) chain isoform X1
XM_015474404.2	XP_015329890.2	collagen alpha-1(XXII) chain isoform X3
XM_025002008.1	XP_024857776.1	collagen alpha-1(XXII) chain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL22A1	XP_528236.3
	LOC100425803	XP_002805544.1
Canis lupus familiaris (dog)	COL22A1	XP_005628047.1
Gallus gallus (chicken)	COL22A1	NP_001132911.1
Homo sapiens (human)	COL22A1	NP_690848.1
Mus musculus (house mouse)	Col22a1	NP_081450.1
Danio rerio (zebrafish)	prp	XP_002665520.3
Bos taurus (cattle)	COL22A1	XP_002692646.2
Rattus norvegicus (Norway rat)	Col22a1	XP_243609.6
Xenopus tropicalis (tropical clawed frog)	col22a1	XP_004915093.1

Gene Ontology

The following COL22A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL22A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222589	XM_025002008.1 Latest version!	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222590	XM_015474404.2 Latest version!	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa25838	XM_002692600.4 Latest version!	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa25838	XM_010822664.2 Latest version!	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa50351	XM_002692600.5 Latest version!	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa92484	XM_015474404.1 Latest version!	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa92484	XM_015466293.1 Latest version!	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL22A1 ( XM_025002008.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857776.1 Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa222589
Clone ID Related Accession (Same CDS sequence)	XM_025002008.1
Accession Version	XM_025002008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGCCTTGC TGCTGCTCGA CCTGGTAACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857776.1
CDS	496..5178
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLLGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGASGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGVGLRGEMGPPGIPALLLLDLVT

Target ORF information:

RefSeq Version	XM_025002008.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025002008.1

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGCCTTGC TGCTGCTCGA CCTGGTAACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_015474404.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329890.2 Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa222590
Clone ID Related Accession (Same CDS sequence)	XM_015474404.2
Accession Version	XM_015474404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329890.2
CDS	496..5100
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLLGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGASGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKALLLLDLVT

Target ORF information:

RefSeq Version	XM_015474404.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474404.2

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_002692600.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002692646.2 Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

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CloneID	OBa25838
Clone ID Related Accession (Same CDS sequence)	XM_002692600.4 , XM_010822664.2
Accession Version	XM_002692600.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692600.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002692646.2
CDS	497..5341
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLPGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGAAGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGVGLRGEMGPPGIPGQPGEPGYAKDGLPGSPGPQGETGPAGHPGPPGPPGPPGQCDPSQCAYFASLAAARPGNVKGP

Target ORF information:

RefSeq Version	XM_002692600.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002692600.4

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_010822664.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820966.2 Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

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CloneID	OBa25838
Clone ID Related Accession (Same CDS sequence)	XM_002692600.4 , XM_010822664.2
Accession Version	XM_010822664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820966.2
CDS	497..5341
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLPGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGAAGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGVGLRGEMGPPGIPGQPGEPGYAKDGLPGSPGPQGETGPAGHPGPPGPPGPPGQCDPSQCAYFASLAAARPGNVKGP

Target ORF information:

RefSeq Version	XM_010822664.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822664.2

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_002692600.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002692646.3 Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

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CloneID	OBa50351
Clone ID Related Accession (Same CDS sequence)	XM_002692600.5
Accession Version	XM_002692600.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002692600.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002692646.3
CDS	496..5340
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLLGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGASGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGVGLRGEMGPPGIPGQPGEPGYAKDGLPGSPGPQGETGPAGHPGPPGPPGPPGQCDPSQCAYFASLAAARPGNVKGP

Target ORF information:

RefSeq Version	XM_002692600.5
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002692600.5

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCACGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACCGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATAGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCTGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCATCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGGTGA GCGAGGAGCC AAAGGTGACC CAGGGGCACC TGGAGTTGGC 
CTCCGAGGCG AGATGGGACC CCCCGGGATC CCAGGTCAGC CCGGGGAACC TGGCTATGCT 
AAAGATGGGC TTCCCGGGAG TCCCGGGCCT CAAGGAGAGA CAGGACCAGC TGGACACCCC 
GGCCCCCCAG GTCCCCCCGG CCCCCCGGGC CAGTGCGACC CCTCACAGTG TGCCTACTTC 
GCCAGCCTTG CTGCTGCTCG ACCTGGTAAC GTGAAGGGCC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_015474404.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329890.1 Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

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CloneID	OBa92484
Clone ID Related Accession (Same CDS sequence)	XM_015474404.1 , XM_015466293.1
Accession Version	XM_015474404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329890.1
CDS	497..5101
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLPGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGAAGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKALLLLDLVT

Target ORF information:

RefSeq Version	XM_015474404.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474404.1

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_015466293.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321779.1 Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

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CloneID	OBa92484
Clone ID Related Accession (Same CDS sequence)	XM_015474404.1 , XM_015466293.1
Accession Version	XM_015466293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XXII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321779.1
CDS	497..5101
Translation	MLVPKHERAMGHLRGSVVAGLLWMLLLWSGDRGCQAQRAGCKTVHYDLVFVLDSSSSVGKENFEKVRQWVANLVDTFEVGPERTRVGVVRYSDRPATAFELGRFGSRAAVRAAARQLAYHGGHTHTGDALRFITRHSFTPRAGGRPGDRAFKQVAILLTDGRSQDLVLPAATAARRAGIRIFAVGVGEALREELEEIASEPTAAHVFHVSDFDAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGTKEITGFDLMDLFSVKTLLGERENGAQSSYVRMGSFPVVQRTEDVFPQGLPDEYAFVTTFRLRKPSRREDWYIWQVIDQYGIPQVSIRLDGENKAVEYSAVGAMKDAVRVVFRGPRVHDLFDRDWHKVALSVQAQNASLYVDCALVQTLPLEERENIDIQGKTAIGKRLYDSVPVDFDLQRVVIYCDSRHAELETCCDIPSGPCQVTVLTEPSPLPQPPPTSGSEQIGFLKTINCSCPPGEKGEKGFDGPVGLPGPKGDMGATGPAGAPGPKGEKGDTGGGPFVQGEKGEKGSLGLPGPPGRDGSKGMRGEPGEPGEPGLPGEVGLRGSQGPPGLPGPPGPIGAPGLHGERGEKGARGEKGEQGLDGFPGKQGETGEQGRPGPPGEPGPRGEKGAVGPAGPPGLPGSVVQREGLKGEQGAPGPRGHQGPPGPPGAPGLMGPEGKDGPPGLQGLRGKRGEAGPPGVPGLLGQQGPPGPPGVPGPPGPGGPPGLPGEIGFPGKPGPPGQAGPPGKDGPNGPPGLPGPKGDPGDRGEDGLPGQRGPRGEVGEQGLAGRPGEKGEAGLPGFPGLPGARGEKGDQGEKGALGLPGLKGDQGVKGEVGPPGPPGLPGTTSLFTPHPRMPGEPGPRGEKGDPGVPGEPGSPGHPGELGPRGPTGPPGAKGQEGAQGAPGAAGSPGVPGLVGPPGPSGPPGSVGPPGSRGPPGKDGERGEKGAAGEEGSPGPAGPRGDPGSPGPPGPPGRGKDGDPGLRGPPGLPGPLGTKGDRGAPGIPGSPGNRGEPGMAVAGPPGPSGPPGEKGPPGSRGPPGFPGPQGPAGQDGIPGNPGERGPPGKPGPSSVLSPGDVNLLVKDVCNDCPPGPPGLPGLPGFKGDKGLPGKPGREGTEGKKGDAGPRGLPGPPGIAGPQGNKGEHGADGEVGQKGDQGHPGVPGFMGPPGDPGPPGADGIAGTAGPPGIQGPPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKTGEPGLPGPEGARGPPGFKGHTGDSGPPGPRGEPGATGPPGREGSPGKDGDTGPTGPQGPQGLRGLPGKSGSPGSPGEPGTPGSPGQKGTKGENGSPGLPGFLGPRGPQGEPGEKGVPGKEGAPGKPGEPGLRGERGDPGIKGDKGPPGGKGQPGDPGTPGHKGHTGLMGPQGPPGESGPAGPPGPPGQPGFPGLRGESPSMDILRRLIQEELEKQLETKLAYLLAQMPSAHTKPSQGRPGPPGPPGKDGLPGRAGPVGEPGRPGQGGLEGPSGPIGPKALLLLDLVT

Target ORF information:

RefSeq Version	XM_015466293.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XXII alpha 1 (COL22A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466293.1

ORF Insert Sequence:

ATGCTGGTTC CCAAGCACGA GAGAGCCATG GGCCACCTGA GAGGGAGCGT CGTGGCCGGC 
CTCCTGTGGA TGCTGCTGCT TTGGAGCGGG GACCGCGGCT GTCAGGCCCA GAGGGCAGGT 
TGCAAAACTG TCCACTATGA CCTGGTGTTC GTGCTGGACT CCTCCTCGAG CGTGGGCAAA 
GAGAACTTCG AGAAGGTCCG GCAGTGGGTG GCCAACCTGG TGGACACGTT CGAGGTGGGC 
CCGGAGCGCA CGCGGGTGGG CGTGGTGCGC TACAGCGACC GCCCAGCCAC AGCCTTCGAG 
CTGGGCCGCT TCGGCTCGCG GGCTGCGGTG CGCGCGGCGG CGCGGCAGCT GGCCTACCAC 
GGCGGCCACA CGCACACGGG CGACGCGCTG CGCTTCATCA CACGCCACAG CTTCACGCCA 
CGAGCCGGCG GCCGCCCCGG GGACCGCGCC TTCAAGCAGG TGGCCATCCT GCTGACCGAC 
GGCCGCAGCC AGGACCTGGT GCTGCCCGCT GCCACGGCCG CGCGCCGTGC TGGCATCCGC 
ATCTTTGCCG TGGGCGTGGG CGAGGCGCTG CGCGAGGAGC TAGAGGAGAT CGCCTCCGAG 
CCCACAGCGG CGCATGTCTT TCACGTGTCT GACTTCGACG CCATCGACAA GATCCGGGGC 
AAGCTGCGGC GCCGCCTGTG CGAGAATGTG CTCTGCCCCA GCGTTCGTGT GGAAGGAGAT 
CGCTTTAAGC ACACCAATGG CGGAACCAAG GAGATCACAG GTTTTGACCT GATGGATTTG 
TTCAGTGTGA AGACGCTCTT GGGGGAGAGA GAGAACGGGG CGCAGAGCTC CTACGTCCGG 
ATGGGATCCT TCCCTGTGGT GCAGAGGACG GAGGACGTGT TCCCGCAAGG TTTGCCCGAT 
GAGTACGCCT TTGTCACCAC CTTCCGGCTC AGGAAACCAT CCCGGAGAGA AGACTGGTAT 
ATCTGGCAGG TCATCGACCA GTACGGCATC CCGCAGGTGT CCATCCGCCT GGATGGCGAG 
AACAAGGCCG TGGAGTACAG CGCTGTGGGC GCCATGAAGG ATGCCGTCAG GGTGGTCTTC 
CGGGGCCCCC GGGTCCACGA CCTCTTCGAC CGGGACTGGC ACAAGGTGGC CCTGAGCGTG 
CAGGCCCAGA ACGCTTCCTT GTACGTCGAC TGTGCACTGG TGCAGACGCT GCCCCTCGAG 
GAGCGGGAGA ACATCGACAT CCAGGGCAAG ACGGCGATCG GCAAGCGCCT CTACGACAGC 
GTGCCCGTCG ACTTTGACCT CCAGCGGGTC GTGATTTACT GCGACTCCCG ACACGCGGAA 
CTGGAGACCT GCTGTGACAT CCCCTCGGGT CCGTGTCAGG TGACTGTGCT GACGGAACCC 
TCCCCGCTGC CCCAGCCGCC TCCCACCTCC GGCAGTGAGC AGATCGGGTT TTTGAAGACC 
ATCAACTGCT CCTGCCCACC TGGAGAAAAG GGTGAGAAGG GCTTCGATGG CCCCGTGGGA 
CTCCCCGGCC CAAAGGGAGA CATGGGAGCC ACTGGGCCAG CAGGCGCTCC TGGACCCAAG 
GGAGAGAAAG GAGATACGGG CGGAGGACCT TTTGTGCAAG GAGAAAAGGG TGAAAAGGGC 
TCCCTGGGCC TGCCGGGTCC CCCAGGAAGA GACGGCAGCA AAGGCATGCG GGGGGAGCCA 
GGAGAACCGG GCGAGCCGGG GCTGCCCGGG GAGGTGGGCC TGCGGGGATC CCAGGGACCG 
CCTGGACTTC CAGGACCTCC CGGCCCCATC GGTGCTCCCG GTCTCCATGG AGAACGAGGG 
GAAAAGGGAG CCCGGGGAGA AAAGGGGGAG CAAGGCCTGG ATGGATTCCC CGGGAAGCAG 
GGGGAGACTG GTGAGCAGGG AAGACCCGGC CCTCCCGGTG AGCCAGGGCC CCGGGGGGAG 
AAGGGTGCCG TGGGTCCTGC AGGCCCCCCT GGGCTTCCAG GCTCTGTGGT GCAGAGAGAG 
GGCCTGAAAG GCGAGCAGGG AGCCCCCGGA CCTCGGGGTC ACCAGGGCCC ACCTGGGCCA 
CCAGGCGCCC CGGGTCTGAT GGGTCCAGAA GGCAAGGATG GACCTCCTGG TTTGCAAGGT 
CTCCGAGGAA AGAGAGGTGA GGCGGGTCCT CCTGGAGTCC CTGGCTTGCT GGGGCAGCAG 
GGCCCCCCAG GACCACCTGG CGTCCCAGGC CCCCCTGGAC CCGGAGGCCC CCCGGGTCTG 
CCTGGAGAGA TCGGCTTCCC GGGAAAGCCT GGGCCCCCTG GGCAGGCGGG ACCGCCTGGA 
AAGGATGGGC CGAATGGACC CCCAGGCCTG CCAGGACCCA AGGGAGACCC AGGAGACAGA 
GGGGAGGACG GTCTGCCCGG ACAGCGCGGC CCGCGGGGTG AAGTTGGGGA GCAGGGCCTG 
GCTGGCCGAC CTGGAGAAAA GGGAGAGGCA GGGCTCCCAG GCTTCCCGGG ACTCCCAGGT 
GCGAGGGGAG AGAAAGGCGA CCAGGGAGAG AAAGGTGCCC TGGGGCTTCC GGGACTGAAA 
GGCGACCAAG GTGTAAAGGG GGAAGTGGGC CCACCAGGGC CCCCTGGATT ACCTGGCACT 
ACTTCCCTGT TCACACCACA CCCGAGAATG CCCGGAGAAC CAGGGCCCAG GGGAGAGAAG 
GGCGATCCTG GCGTGCCTGG GGAGCCGGGG TCGCCAGGTC ATCCTGGAGA ACTGGGGCCT 
CGGGGACCCA CTGGACCACC GGGCGCCAAG GGACAAGAAG GCGCCCAGGG AGCGCCTGGA 
GCAGCCGGGA GCCCCGGAGT TCCTGGACTT GTAGGGCCCC CTGGCCCCAG CGGCCCCCCA 
GGGAGCGTGG GTCCCCCGGG CTCCAGAGGC CCCCCTGGGA AAGATGGGGA GCGTGGTGAG 
AAGGGAGCAG CAGGAGAAGA AGGGAGCCCA GGGCCAGCCG GCCCCAGGGG CGATCCTGGT 
TCCCCCGGGC CCCCCGGGCC CCCTGGAAGA GGGAAAGATG GAGACCCGGG ACTTCGTGGC 
CCACCTGGAT TGCCAGGACC TCTGGGAACC AAGGGGGACC GTGGTGCGCC TGGGATCCCC 
GGCTCTCCTG GCAACCGTGG AGAGCCAGGC ATGGCGGTCG CCGGCCCTCC GGGCCCCTCT 
GGACCACCAG GAGAAAAAGG TCCTCCGGGA TCTCGAGGCC CGCCTGGGTT TCCCGGCCCC 
CAGGGACCAG CTGGCCAGGA TGGTATACCA GGAAATCCAG GAGAAAGAGG GCCTCCTGGC 
AAACCAGGCC CCTCTTCAGT ACTGTCTCCA GGGGACGTAA ATCTCTTGGT GAAGGACGTG 
TGCAATGACT GTCCCCCGGG TCCCCCAGGC CTCCCTGGTC TTCCAGGTTT TAAAGGGGAC 
AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GGAAAAAGGG GGATGCTGGG 
CCCAGAGGCC TCCCAGGGCC CCCAGGAATA GCTGGACCTC AGGGAAATAA AGGAGAACAT 
GGTGCAGACG GTGAGGTTGG ACAGAAAGGT GACCAGGGTC ATCCCGGAGT TCCCGGTTTC 
ATGGGCCCCC CAGGGGACCC TGGACCACCA GGAGCAGACG GAATAGCGGG AACTGCTGGA 
CCACCAGGAA TCCAAGGACC CCCAGGGAAA GAAGGTCCTC CTGGCCCCCA AGGCCCGTCT 
GGATTACCCG GCATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGGCCC 
CCCGGAGAGC CGGGCAAAAC AGGAGAGCCG GGTCTGCCAG GACCAGAGGG TGCCCGCGGT 
CCCCCTGGCT TCAAGGGACA CACAGGCGAC TCTGGCCCAC CTGGCCCCCG GGGAGAGCCT 
GGCGCCACGG GGCCCCCTGG CCGGGAGGGG TCACCAGGGA AAGACGGTGA CACTGGACCT 
ACAGGGCCAC AGGGTCCCCA AGGACTGAGG GGCCTACCGG GTAAGAGTGG GTCACCTGGA 
TCACCGGGAG AGCCTGGCAC CCCAGGAAGC CCTGGCCAGA AGGGAACCAA AGGAGAAAAC 
GGCAGCCCAG GGCTCCCGGG CTTCCTGGGT CCCCGGGGGC CTCAGGGGGA GCCAGGCGAG 
AAAGGAGTCC CAGGCAAGGA GGGGGCACCT GGGAAGCCAG GAGAGCCAGG ACTCAGAGGA 
GAAAGGGGAG ATCCTGGCAT CAAAGGTGAC AAAGGCCCTC CCGGTGGCAA GGGCCAGCCC 
GGCGACCCCG GGACCCCAGG CCACAAGGGT CACACAGGCC TCATGGGCCC CCAGGGGCCA 
CCTGGGGAGA GCGGGCCGGC CGGTCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGG 
CTAAGGGGCG AGTCTCCATC CATGGACATC CTGCGGCGGC TCATCCAAGA GGAACTGGAG 
AAACAGCTGG AAACCAAACT CGCCTACCTC CTGGCCCAGA TGCCCTCTGC ACACACGAAG 
CCCTCTCAAG GCAGACCCGG ACCCCCAGGG CCTCCTGGAA AAGATGGGCT TCCAGGTCGG 
GCAGGCCCCG TCGGGGAGCC AGGAAGGCCA GGGCAAGGGG GTCTGGAGGG ACCCTCTGGC 
CCCATAGGTC CCAAAGCCTT GCTGCTGCTC GACCTGGTAA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL22A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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