PTCH1 cDNA ORF clone, Bos taurus(cattle)

The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237096 XM_024995742.1
Latest version!
Bos taurus patched 1 (PTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa22272 XM_010808162.3
Latest version!
Bos taurus patched 1 (PTCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22272 XM_005210407.4
Latest version!
Bos taurus patched 1 (PTCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa39601 NM_001205879.2
Latest version!
Bos taurus patched 1 (PTCH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa22271 XM_005210406.4
Latest version!
Bos taurus patched 1 (PTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa22272 XM_005210407.3 Bos taurus patched 1 (PTCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa39601 NM_001205879.1 Bos taurus patched 1 (PTCH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa22271 XM_005210406.3 Bos taurus patched 1 (PTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa22272 XM_010808162.2 Bos taurus patched 1 (PTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237096
    Clone ID Related Accession (Same CDS sequence) XM_024995742.1
    Accession Version XM_024995742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAAG CTACTGGCCG GAAAGCGCCG CTGTGGCTGC GAGCGAAGTT TCAAAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTAGGCCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATTC AGACCCCCAA GGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCGA CAGCACCTGG ACTCGGCGCT CCAGGCCAGC
    CGGGTCCATG TATACATGTA TAACAGGCAG TGGAAATTGG AACATTTGTG TTATAAATCT
    GGAGAACTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAGTATCT TTACCCTTGT
    TTAATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AGTTACAGTC TGGGACGGCA
    TACCTACTAG GTAAGCCTCC TTTGCAGTGG ACAAACTTTG ACCCTTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAA
    GTTGGTCATG GTTACATGGA CCGGCCCTGC CTCAATCCAG CTGATCCAGA CTGTCCCGCC
    ACAGCCCCCA ACAAAAATGC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGG
    TGTCATGGAT TATCCAGAAA GTATATGCAC TGGCAGGAGG AACTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGGAA GCTTGTCAGT GCCCATGCTC TGCAGACCAT GTTTCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AAGGGTTATG AGTATGTCTC GCACATCAAC
    TGGAACGAGG ACAAGGCAGC AGCCATCCTG GAGGCTTGGC AAAGGACGTA CGTGGAGGTG
    GTTCATCAGA GTGTTGCTCA GAACTCCACT CAGAAGGTGC TTTCCTTTAC CACGACGACC
    CTGGATGACA TCCTCAAGTC CTTCTCTGAT GTCAGCGTCA TCCGAGTGGC CAGCGGCTAT
    TTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAGGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTGTGCTCGC TGATCGGGAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCGTTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTT CTTCTGGCGC ATGCATTTAG TGAAACGGGA
    CAGAATAAAA GAATTCCTTT TGAGGACAGG ACCGGGGAGT GCCTCAAGCG CACGGGAGCC
    AGTGTGGCCC TCACCTCCAT CAGCAACGTC ACAGCCTTCT TCATGGCCGC GTTGATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTGCAG GCGGCTGTAG TAGTGGTGTT CAATTTTGCT
    ATGGTTCTGC TGATTTTTCC TGCAATTCTC AGCATGGATT TGTATCGACG TGAGGACAGG
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGAGTGAT TCAGGTTGAA
    CCCCAGGCCT ACACAGAGAC TCACGATAAC ACCCGCTACA GCCCCCCACC CCCCTACAGC
    AGCCACGGCT TCGCCCATGA AACCCAGATC ACCATGCAGT CCACGGTACA GCTTCGCACA
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACTGCCG AGCCACGCTC CGAGATCTCT
    GTGCAGCCCG TCACCATGGC TCAGGACACA CTCAGCTGCC AGAGCCCCGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCG GACTCCAGCC TCCACTGCCT GGAGCCCCCC
    TGCACCAGGT GGACACTTTC ATCTTTCGCT GAGAAGCACT ATGCTCCTTT CCTCTTAAAA
    CCAAAAGCCA AGGTCGTGGT GATCTTCCTT TTCCTGGGCC TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGCGTGA TGGGCTGGAT CTTACAGACA TTGTGCCTCG GGAAACTAGA
    GAATATGACT TTATTGCTGC ACAGTTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACTCAGAAAG CAGATTACCC GAATATCCAG CACTTACTTT ATGACCTTCA TAAGAGTTTC
    AGTAATGTGA AGTATGTCAT GCTGGAAGAA AATAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTCCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAAAATGGA TCGGATGATG GGGTCCTTGC CTACAAACTC
    CTGGTACAAA CCGGCAGCCG TGATAAGCCC ATTGACATCA GTCAGCTGAC TAAACGGCGC
    CTGGTGGATG CAGACGGCAT CATTAACCCC AGTGCTTTCT ACATCTACCT GACTGCTTGG
    GTCAGCAACG ACCCTGTGGC TTATGCCGCC TCCCAAGCCA ACATCCGGCC TCACCGTCCT
    GAGTGGGTTC ATGACAAAGC CGACTACATG CCAGAAACCA GGCTGAGAAT CCCCGCGGCA
    GAGCCCATTG AATATGCTCA GTTCCCTTTC TACCTCAACG GCCTGCGTGA CACCTCGGAC
    TTCGTTGAAG CCATCGAAAA AGTCAGAACC ATCTGCAATA ACTACACGAG CCTGGGGCTC
    TCCAGCTACC CCAATGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATTGG CCTCCGCCAC
    TGGCTCCTCC TGTCCATCAG CGTGGTGCTG GCCTGCACGT TCCTCGTGTG CGCCGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTGACAGTCT TGGCTTTGAT GACAGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGAATC AAGCTGAGTG CCGTGCCTGT GGTCATCTTG
    ATTGCTTCCG TCGGCATTGG AGTGGAATTC ACTGTTCATG TCGCTCTGGC CTTTCTAACA
    GCCATTGGTG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCGCCTGTT
    CTCGATGGTG CCGTTTCCAC TCTACTGGGA GTGCTGATGC TTGCAGGATC TGAATTTGAT
    TTCATTGTCA GGTACTTCTT TGCTGTCCTG GCAATCCTAA CAATCCTGGG TGTTCTCAAT
    GGACTAGTTT TGCTGCCAGT CCTTTTGTCC TTCTTCGGAC CGTATCCTGA GGTGTCTCCA
    GCCAATGGGC TGAACCGGCT GCCCACACCT TCTCCTGAGC CACCCCCCAG CGTGGTCCGG
    TTTGCTGTGC CCGCTGGTCA CACAAACAAC GGGTCTGATT CCTCTGACTC GGAGTACAGT
    TCTCAGACGA CCGTGTCAGG CATCAGTGAG GAGCTGCGGC ACTACGAGGC CCAGCAGGGT
    GCTGGGGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCTGT CTTCGCCCGC
    TCCACTGTGG TCCATCCTGA ACCGAGGCAT CACCCACCCT CAAATCCTCG ACAGCAGTCC
    CACCTGGACT CAAGGACCCT GCCACCTGGA CAGCCAGGAC AGCAGCCCAG AAGAGAAACC
    CCCAGAGAAT GCTTGAGGGC GCCTCCATAT AAACCGCGCA GAGACGCTTT CGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCACGG GCCCCCGGGG AGCCCGTTCT
    CACAACCCTC GGCATCACAA CCCAGCGTTC ACTGCCATGG GCAGCTCCAT GCCCAGCTAC
    TGCCAGCCCA TTACCACCGT GACGGCCTCG GCGTCCGTGA CTGTTGCCGT GCATCCTCCG
    CCCGCCCCTG GGCCTGGGCC CAGTAGGAAC CCCCGAAGCG GGCTTTGCCC AGGCTACGAG
    GACTACCCCG AGACTGACCA CGGCCTGTTT GAGGACCCCC ATGTGCCTTT TAATGTCCGG
    TGTGAGAGGA GGGAACCCAA GGTGGAGGTC ATTGAGCTGC AGGACGTGGA ATGTGAGGAG
    AGACGCCACG GCAGCAGCTC CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851510.1
    CDS445..4611
    Translation

    Target ORF information:

    RefSeq Version XM_024995742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAAG CTACTGGCCG GAAAGCGCCG CTGTGGCTGC GAGCGAAGTT TCAAAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTAGGCCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATTC AGACCCCCAA GGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCGA CAGCACCTGG ACTCGGCGCT CCAGGCCAGC
    CGGGTCCATG TATACATGTA TAACAGGCAG TGGAAATTGG AACATTTGTG TTATAAATCT
    GGAGAACTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAGTATCT TTACCCTTGT
    TTAATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AGTTACAGTC TGGGACGGCA
    TACCTACTAG GTAAGCCTCC TTTGCAGTGG ACAAACTTTG ACCCTTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAA
    GTTGGTCATG GTTACATGGA CCGGCCCTGC CTCAATCCAG CTGATCCAGA CTGTCCCGCC
    ACAGCCCCCA ACAAAAATGC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGG
    TGTCATGGAT TATCCAGAAA GTATATGCAC TGGCAGGAGG AACTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGGAA GCTTGTCAGT GCCCATGCTC TGCAGACCAT GTTTCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AAGGGTTATG AGTATGTCTC GCACATCAAC
    TGGAACGAGG ACAAGGCAGC AGCCATCCTG GAGGCTTGGC AAAGGACGTA CGTGGAGGTG
    GTTCATCAGA GTGTTGCTCA GAACTCCACT CAGAAGGTGC TTTCCTTTAC CACGACGACC
    CTGGATGACA TCCTCAAGTC CTTCTCTGAT GTCAGCGTCA TCCGAGTGGC CAGCGGCTAT
    TTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAGGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTGTGCTCGC TGATCGGGAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCGTTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTT CTTCTGGCGC ATGCATTTAG TGAAACGGGA
    CAGAATAAAA GAATTCCTTT TGAGGACAGG ACCGGGGAGT GCCTCAAGCG CACGGGAGCC
    AGTGTGGCCC TCACCTCCAT CAGCAACGTC ACAGCCTTCT TCATGGCCGC GTTGATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTGCAG GCGGCTGTAG TAGTGGTGTT CAATTTTGCT
    ATGGTTCTGC TGATTTTTCC TGCAATTCTC AGCATGGATT TGTATCGACG TGAGGACAGG
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGAGTGAT TCAGGTTGAA
    CCCCAGGCCT ACACAGAGAC TCACGATAAC ACCCGCTACA GCCCCCCACC CCCCTACAGC
    AGCCACGGCT TCGCCCATGA AACCCAGATC ACCATGCAGT CCACGGTACA GCTTCGCACA
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACTGCCG AGCCACGCTC CGAGATCTCT
    GTGCAGCCCG TCACCATGGC TCAGGACACA CTCAGCTGCC AGAGCCCCGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCG GACTCCAGCC TCCACTGCCT GGAGCCCCCC
    TGCACCAGGT GGACACTTTC ATCTTTCGCT GAGAAGCACT ATGCTCCTTT CCTCTTAAAA
    CCAAAAGCCA AGGTCGTGGT GATCTTCCTT TTCCTGGGCC TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGCGTGA TGGGCTGGAT CTTACAGACA TTGTGCCTCG GGAAACTAGA
    GAATATGACT TTATTGCTGC ACAGTTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACTCAGAAAG CAGATTACCC GAATATCCAG CACTTACTTT ATGACCTTCA TAAGAGTTTC
    AGTAATGTGA AGTATGTCAT GCTGGAAGAA AATAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTCCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAAAATGGA TCGGATGATG GGGTCCTTGC CTACAAACTC
    CTGGTACAAA CCGGCAGCCG TGATAAGCCC ATTGACATCA GTCAGCTGAC TAAACGGCGC
    CTGGTGGATG CAGACGGCAT CATTAACCCC AGTGCTTTCT ACATCTACCT GACTGCTTGG
    GTCAGCAACG ACCCTGTGGC TTATGCCGCC TCCCAAGCCA ACATCCGGCC TCACCGTCCT
    GAGTGGGTTC ATGACAAAGC CGACTACATG CCAGAAACCA GGCTGAGAAT CCCCGCGGCA
    GAGCCCATTG AATATGCTCA GTTCCCTTTC TACCTCAACG GCCTGCGTGA CACCTCGGAC
    TTCGTTGAAG CCATCGAAAA AGTCAGAACC ATCTGCAATA ACTACACGAG CCTGGGGCTC
    TCCAGCTACC CCAATGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATTGG CCTCCGCCAC
    TGGCTCCTCC TGTCCATCAG CGTGGTGCTG GCCTGCACGT TCCTCGTGTG CGCCGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTGACAGTCT TGGCTTTGAT GACAGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGAATC AAGCTGAGTG CCGTGCCTGT GGTCATCTTG
    ATTGCTTCCG TCGGCATTGG AGTGGAATTC ACTGTTCATG TCGCTCTGGC CTTTCTAACA
    GCCATTGGTG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCGCCTGTT
    CTCGATGGTG CCGTTTCCAC TCTACTGGGA GTGCTGATGC TTGCAGGATC TGAATTTGAT
    TTCATTGTCA GGTACTTCTT TGCTGTCCTG GCAATCCTAA CAATCCTGGG TGTTCTCAAT
    GGACTAGTTT TGCTGCCAGT CCTTTTGTCC TTCTTCGGAC CGTATCCTGA GGTGTCTCCA
    GCCAATGGGC TGAACCGGCT GCCCACACCT TCTCCTGAGC CACCCCCCAG CGTGGTCCGG
    TTTGCTGTGC CCGCTGGTCA CACAAACAAC GGGTCTGATT CCTCTGACTC GGAGTACAGT
    TCTCAGACGA CCGTGTCAGG CATCAGTGAG GAGCTGCGGC ACTACGAGGC CCAGCAGGGT
    GCTGGGGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCTGT CTTCGCCCGC
    TCCACTGTGG TCCATCCTGA ACCGAGGCAT CACCCACCCT CAAATCCTCG ACAGCAGTCC
    CACCTGGACT CAAGGACCCT GCCACCTGGA CAGCCAGGAC AGCAGCCCAG AAGAGAAACC
    CCCAGAGAAT GCTTGAGGGC GCCTCCATAT AAACCGCGCA GAGACGCTTT CGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCACGG GCCCCCGGGG AGCCCGTTCT
    CACAACCCTC GGCATCACAA CCCAGCGTTC ACTGCCATGG GCAGCTCCAT GCCCAGCTAC
    TGCCAGCCCA TTACCACCGT GACGGCCTCG GCGTCCGTGA CTGTTGCCGT GCATCCTCCG
    CCCGCCCCTG GGCCTGGGCC CAGTAGGAAC CCCCGAAGCG GGCTTTGCCC AGGCTACGAG
    GACTACCCCG AGACTGACCA CGGCCTGTTT GAGGACCCCC ATGTGCCTTT TAATGTCCGG
    TGTGAGAGGA GGGAACCCAA GGTGGAGGTC ATTGAGCTGC AGGACGTGGA ATGTGAGGAG
    AGACGCCACG GCAGCAGCTC CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22272
    Clone ID Related Accession (Same CDS sequence) XM_005210407.3 , XM_010808162.2 , XM_005210407.4 , XM_010808162.3
    Accession Version XM_005210407.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104131.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210407.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210464.1
    CDS334..4245
    Translation

    Target ORF information:

    RefSeq Version XM_005210407.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210407.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22272
    Clone ID Related Accession (Same CDS sequence) XM_005210407.3 , XM_010808162.2 , XM_005210407.4 , XM_010808162.3
    Accession Version XM_010808162.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104131.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806464.1
    CDS159..4070
    Translation

    Target ORF information:

    RefSeq Version XM_010808162.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808162.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22272
    Clone ID Related Accession (Same CDS sequence) XM_005210407.3 , XM_010808162.2 , XM_005210407.4 , XM_010808162.3
    Accession Version XM_005210407.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210407.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210464.1
    CDS1147..5058
    Translation

    Target ORF information:

    RefSeq Version XM_005210407.4
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210407.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22272
    Clone ID Related Accession (Same CDS sequence) XM_005210407.3 , XM_010808162.2 , XM_005210407.4 , XM_010808162.3
    Accession Version XM_010808162.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806464.1
    CDS177..4088
    Translation

    Target ORF information:

    RefSeq Version XM_010808162.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808162.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTTAATC CTCAACTCAT GATTCAGACC CCCAAGGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCGACAGCA CCTGGACTCG GCGCTCCAGG CCAGCCGGGT CCATGTATAC
    ATGTATAACA GGCAGTGGAA ATTGGAACAT TTGTGTTATA AATCTGGAGA ACTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAG TATCTTTACC CTTGTTTAAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAGTTA CAGTCTGGGA CGGCATACCT ACTAGGTAAG
    CCTCCTTTGC AGTGGACAAA CTTTGACCCT TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAAGTTGG TCATGGTTAC
    ATGGACCGGC CCTGCCTCAA TCCAGCTGAT CCAGACTGTC CCGCCACAGC CCCCAACAAA
    AATGCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGGTGTCA TGGATTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAACTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG ACCATGTTTC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAAGGG TTATGAGTAT GTCTCGCACA TCAACTGGAA CGAGGACAAG
    GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG ACGTACGTGG AGGTGGTTCA TCAGAGTGTT
    GCTCAGAACT CCACTCAGAA GGTGCTTTCC TTTACCACGA CGACCCTGGA TGACATCCTC
    AAGTCCTTCT CTGATGTCAG CGTCATCCGA GTGGCCAGCG GCTATTTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTGTG CTCGCTGATC
    GGGATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TCTTTCTTCT GGCGCATGCA TTTAGTGAAA CGGGACAGAA TAAAAGAATT
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTC AAGCGCACGG GAGCCAGTGT GGCCCTCACC
    TCCATCAGCA ACGTCACAGC CTTCTTCATG GCCGCGTTGA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG GTGTTCAATT TTGCTATGGT TCTGCTGATT
    TTTCCTGCAA TTCTCAGCAT GGATTTGTAT CGACGTGAGG ACAGGAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA GTGATTCAGG TTGAACCCCA GGCCTACACA
    GAGACTCACG ATAACACCCG CTACAGCCCC CCACCCCCCT ACAGCAGCCA CGGCTTCGCC
    CATGAAACCC AGATCACCAT GCAGTCCACG GTACAGCTTC GCACAGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC TGCCGAGCCA CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    ATGGCTCAGG ACACACTCAG CTGCCAGAGC CCCGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCGGACTC CAGCCTCCAC TGCCTGGAGC CCCCCTGCAC CAGGTGGACA
    CTTTCATCTT TCGCTGAGAA GCACTATGCT CCTTTCCTCT TAAAACCAAA AGCCAAGGTC
    GTGGTGATCT TCCTTTTCCT GGGCCTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    CGTGATGGGC TGGATCTTAC AGACATTGTG CCTCGGGAAA CTAGAGAATA TGACTTTATT
    GCTGCACAGT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACTCA GAAAGCAGAT
    TACCCGAATA TCCAGCACTT ACTTTATGAC CTTCATAAGA GTTTCAGTAA TGTGAAGTAT
    GTCATGCTGG AAGAAAATAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTCCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAAA ATGGATCGGA TGATGGGGTC CTTGCCTACA AACTCCTGGT ACAAACCGGC
    AGCCGTGATA AGCCCATTGA CATCAGTCAG CTGACTAAAC GGCGCCTGGT GGATGCAGAC
    GGCATCATTA ACCCCAGTGC TTTCTACATC TACCTGACTG CTTGGGTCAG CAACGACCCT
    GTGGCTTATG CCGCCTCCCA AGCCAACATC CGGCCTCACC GTCCTGAGTG GGTTCATGAC
    AAAGCCGACT ACATGCCAGA AACCAGGCTG AGAATCCCCG CGGCAGAGCC CATTGAATAT
    GCTCAGTTCC CTTTCTACCT CAACGGCCTG CGTGACACCT CGGACTTCGT TGAAGCCATC
    GAAAAAGTCA GAACCATCTG CAATAACTAC ACGAGCCTGG GGCTCTCCAG CTACCCCAAT
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATTGGCCTCC GCCACTGGCT CCTCCTGTCC
    ATCAGCGTGG TGCTGGCCTG CACGTTCCTC GTGTGCGCCG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAC AGTCTTGGCT TTGATGACAG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GAATCAAGCT GAGTGCCGTG CCTGTGGTCA TCTTGATTGC TTCCGTCGGC
    ATTGGAGTGG AATTCACTGT TCATGTCGCT CTGGCCTTTC TAACAGCCAT TGGTGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT
    TCCACTCTAC TGGGAGTGCT GATGCTTGCA GGATCTGAAT TTGATTTCAT TGTCAGGTAC
    TTCTTTGCTG TCCTGGCAAT CCTAACAATC CTGGGTGTTC TCAATGGACT AGTTTTGCTG
    CCAGTCCTTT TGTCCTTCTT CGGACCGTAT CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC
    CGGCTGCCCA CACCTTCTCC TGAGCCACCC CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT
    GGTCACACAA ACAACGGGTC TGATTCCTCT GACTCGGAGT ACAGTTCTCA GACGACCGTG
    TCAGGCATCA GTGAGGAGCT GCGGCACTAC GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT
    CCTGAACCGA GGCATCACCC ACCCTCAAAT CCTCGACAGC AGTCCCACCT GGACTCAAGG
    ACCCTGCCAC CTGGACAGCC AGGACAGCAG CCCAGAAGAG AAACCCCCAG AGAATGCTTG
    AGGGCGCCTC CATATAAACC GCGCAGAGAC GCTTTCGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CACGGGCCCC CGGGGAGCCC GTTCTCACAA CCCTCGGCAT
    CACAACCCAG CGTTCACTGC CATGGGCAGC TCCATGCCCA GCTACTGCCA GCCCATTACC
    ACCGTGACGG CCTCGGCGTC CGTGACTGTT GCCGTGCATC CTCCGCCCGC CCCTGGGCCT
    GGGCCCAGTA GGAACCCCCG AAGCGGGCTT TGCCCAGGCT ACGAGGACTA CCCCGAGACT
    GACCACGGCC TGTTTGAGGA CCCCCATGTG CCTTTTAATG TCCGGTGTGA GAGGAGGGAA
    CCCAAGGTGG AGGTCATTGA GCTGCAGGAC GTGGAATGTG AGGAGAGACG CCACGGCAGC
    AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39601
    Clone ID Related Accession (Same CDS sequence) NM_001205879.2 , NM_001205879.1
    Accession Version NM_001205879.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product protein patched homolog 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On Dec 20, 2018 this sequence version replaced NM_001205879.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGCTTGCC TATGCCTGTT TAACCATGCT GCGCTGGGAC
    TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG
    GCTGCAGGAT TGGGCCTGTG CTCGCTGATC GGGATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TCTTTCTTCT GGCGCATGCA
    TTTAGTGAAA CGGGACAGAA TAAAAGAATT CCTTTTGAGG ACAGGACCGG GGAGTGCCTC
    AAGCGCACGG GAGCCAGTGT GGCCCTCACC TCCATCAGCA ACGTCACAGC CTTCTTCATG
    GCCGCGTTGA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG
    GTGTTCAATT TTGCTATGGT TCTGCTGATT TTTCCTGCAA TTCTCAGCAT GGATTTGTAT
    CGACGTGAGG ACAGGAGATT GGATATTTTC TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA
    GTGATTCAGG TTGAACCCCA GGCCTACACA GAGACTCACG ATAACACCCG CTACAGCCCC
    CCACCCCCCT ACAGCAGCCA CGGCTTCGCC CATGAAACCC AGATCACCAT GCAGTCCACG
    GTACAGCTTC GCACAGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC TGCCGAGCCA
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC ATGGCTCAGG ACACACTCAG CTGCCAGAGC
    CCCGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCGGACTC CAGCCTCCAC
    TGCCTGGAGC CCCCCTGCAC CAGGTGGACA CTTTCATCTT TCGCTGAGAA GCACTATGCT
    CCTTTCCTCT TAAAACCAAA AGCCAAGGTC GTGGTGATCT TCCTTTTCCT GGGCCTGCTG
    GGGGTCAGCC TTTATGGGAC CACCCGAGTG CGTGATGGGC TGGATCTTAC AGACATTGTG
    CCTCGGGAAA CTAGAGAATA TGACTTTATT GCTGCACAGT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACTCA GAAAGCAGAT TACCCGAATA TCCAGCACTT ACTTTATGAC
    CTTCATAAGA GTTTCAGTAA TGTGAAGTAT GTCATGCTGG AAGAAAATAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTCCAAGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAAA ATGGATCGGA TGATGGGGTC
    CTTGCCTACA AACTCCTGGT ACAAACCGGC AGCCGTGATA AGCCCATTGA CATCAGTCAG
    CTGACTAAAC GGCGCCTGGT GGATGCAGAC GGCATCATTA ACCCCAGTGC TTTCTACATC
    TACCTGACTG CTTGGGTCAG CAACGACCCT GTGGCTTATG CCGCCTCCCA AGCCAACATC
    CGGCCTCACC GTCCTGAGTG GGTTCATGAC AAAGCCGACT ACATGCCAGA AACCAGGCTG
    AGAATCCCCG CGGCAGAGCC CATTGAATAT GCTCAGTTCC CTTTCTACCT CAACGGCCTG
    CGTGACACCT CGGACTTCGT TGAAGCCATC GAAAAAGTCA GAACCATCTG CAATAACTAC
    ACGAGCCTGG GGCTCTCCAG CTACCCCAAT GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATTGGCCTCC GCCACTGGCT CCTCCTGTCC ATCAGCGTGG TGCTGGCCTG CACGTTCCTC
    GTGTGCGCCG TCTTCCTCCT GAACCCCTGG ACGGCCGGGA TCATTGTGAC AGTCTTGGCT
    TTGATGACAG TTGAGCTCTT TGGCATGATG GGCCTCATTG GAATCAAGCT GAGTGCCGTG
    CCTGTGGTCA TCTTGATTGC TTCCGTCGGC ATTGGAGTGG AATTCACTGT TCATGTCGCT
    CTGGCCTTTC TAACAGCCAT TGGTGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT TCCACTCTAC TGGGAGTGCT GATGCTTGCA
    GGATCTGAAT TTGATTTCAT TGTCAGGTAC TTCTTTGCTG TCCTGGCAAT CCTAACAATC
    CTGGGTGTTC TCAATGGACT AGTTTTGCTG CCAGTCCTTT TGTCCTTCTT CGGACCGTAT
    CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC CGGCTGCCCA CACCTTCTCC TGAGCCACCC
    CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT GGTCACACAA ACAACGGGTC TGATTCCTCT
    GACTCGGAGT ACAGTTCTCA GACGACCGTG TCAGGCATCA GTGAGGAGCT GCGGCACTAC
    GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT CCTGAACCGA GGCATCACCC ACCCTCAAAT
    CCTCGACAGC AGTCCCACCT GGACTCAAGG ACCCTGCCAC CTGGACAGCC AGGACAGCAG
    CCCAGAAGAG AAACCCCCAG AGAATGCTTG AGGGCGCCTC CATATAAACC GCGCAGAGAC
    GCTTTCGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGACCG CACGGGCCCC
    CGGGGAGCCC GTTCTCACAA CCCTCGGCAT CACAACCCAG CGTTCACTGC CATGGGCAGC
    TCCATGCCCA GCTACTGCCA GCCCATTACC ACCGTGACGG CCTCGGCGTC CGTGACTGTT
    GCCGTGCATC CTCCGCCCGC CCCTGGGCCT GGGCCCAGTA GGAACCCCCG AAGCGGGCTT
    TGCCCAGGCT ACGAGGACTA CCCCGAGACT GACCACGGCC TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TCCGGTGTGA GAGGAGGGAA CCCAAGGTGG AGGTCATTGA GCTGCAGGAC
    GTGGAATGTG AGGAGAGACG CCACGGCAGC AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192808.1
    CDS148..4509
    Translation

    Target ORF information:

    RefSeq Version NM_001205879.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205879.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGCTTGCC TATGCCTGTT TAACCATGCT GCGCTGGGAC
    TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG
    GCTGCAGGAT TGGGCCTGTG CTCGCTGATC GGGATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TCTTTCTTCT GGCGCATGCA
    TTTAGTGAAA CGGGACAGAA TAAAAGAATT CCTTTTGAGG ACAGGACCGG GGAGTGCCTC
    AAGCGCACGG GAGCCAGTGT GGCCCTCACC TCCATCAGCA ACGTCACAGC CTTCTTCATG
    GCCGCGTTGA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG
    GTGTTCAATT TTGCTATGGT TCTGCTGATT TTTCCTGCAA TTCTCAGCAT GGATTTGTAT
    CGACGTGAGG ACAGGAGATT GGATATTTTC TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA
    GTGATTCAGG TTGAACCCCA GGCCTACACA GAGACTCACG ATAACACCCG CTACAGCCCC
    CCACCCCCCT ACAGCAGCCA CGGCTTCGCC CATGAAACCC AGATCACCAT GCAGTCCACG
    GTACAGCTTC GCACAGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC TGCCGAGCCA
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC ATGGCTCAGG ACACACTCAG CTGCCAGAGC
    CCCGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCGGACTC CAGCCTCCAC
    TGCCTGGAGC CCCCCTGCAC CAGGTGGACA CTTTCATCTT TCGCTGAGAA GCACTATGCT
    CCTTTCCTCT TAAAACCAAA AGCCAAGGTC GTGGTGATCT TCCTTTTCCT GGGCCTGCTG
    GGGGTCAGCC TTTATGGGAC CACCCGAGTG CGTGATGGGC TGGATCTTAC AGACATTGTG
    CCTCGGGAAA CTAGAGAATA TGACTTTATT GCTGCACAGT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACTCA GAAAGCAGAT TACCCGAATA TCCAGCACTT ACTTTATGAC
    CTTCATAAGA GTTTCAGTAA TGTGAAGTAT GTCATGCTGG AAGAAAATAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTCCAAGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAAA ATGGATCGGA TGATGGGGTC
    CTTGCCTACA AACTCCTGGT ACAAACCGGC AGCCGTGATA AGCCCATTGA CATCAGTCAG
    CTGACTAAAC GGCGCCTGGT GGATGCAGAC GGCATCATTA ACCCCAGTGC TTTCTACATC
    TACCTGACTG CTTGGGTCAG CAACGACCCT GTGGCTTATG CCGCCTCCCA AGCCAACATC
    CGGCCTCACC GTCCTGAGTG GGTTCATGAC AAAGCCGACT ACATGCCAGA AACCAGGCTG
    AGAATCCCCG CGGCAGAGCC CATTGAATAT GCTCAGTTCC CTTTCTACCT CAACGGCCTG
    CGTGACACCT CGGACTTCGT TGAAGCCATC GAAAAAGTCA GAACCATCTG CAATAACTAC
    ACGAGCCTGG GGCTCTCCAG CTACCCCAAT GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATTGGCCTCC GCCACTGGCT CCTCCTGTCC ATCAGCGTGG TGCTGGCCTG CACGTTCCTC
    GTGTGCGCCG TCTTCCTCCT GAACCCCTGG ACGGCCGGGA TCATTGTGAC AGTCTTGGCT
    TTGATGACAG TTGAGCTCTT TGGCATGATG GGCCTCATTG GAATCAAGCT GAGTGCCGTG
    CCTGTGGTCA TCTTGATTGC TTCCGTCGGC ATTGGAGTGG AATTCACTGT TCATGTCGCT
    CTGGCCTTTC TAACAGCCAT TGGTGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT TCCACTCTAC TGGGAGTGCT GATGCTTGCA
    GGATCTGAAT TTGATTTCAT TGTCAGGTAC TTCTTTGCTG TCCTGGCAAT CCTAACAATC
    CTGGGTGTTC TCAATGGACT AGTTTTGCTG CCAGTCCTTT TGTCCTTCTT CGGACCGTAT
    CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC CGGCTGCCCA CACCTTCTCC TGAGCCACCC
    CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT GGTCACACAA ACAACGGGTC TGATTCCTCT
    GACTCGGAGT ACAGTTCTCA GACGACCGTG TCAGGCATCA GTGAGGAGCT GCGGCACTAC
    GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT CCTGAACCGA GGCATCACCC ACCCTCAAAT
    CCTCGACAGC AGTCCCACCT GGACTCAAGG ACCCTGCCAC CTGGACAGCC AGGACAGCAG
    CCCAGAAGAG AAACCCCCAG AGAATGCTTG AGGGCGCCTC CATATAAACC GCGCAGAGAC
    GCTTTCGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGACCG CACGGGCCCC
    CGGGGAGCCC GTTCTCACAA CCCTCGGCAT CACAACCCAG CGTTCACTGC CATGGGCAGC
    TCCATGCCCA GCTACTGCCA GCCCATTACC ACCGTGACGG CCTCGGCGTC CGTGACTGTT
    GCCGTGCATC CTCCGCCCGC CCCTGGGCCT GGGCCCAGTA GGAACCCCCG AAGCGGGCTT
    TGCCCAGGCT ACGAGGACTA CCCCGAGACT GACCACGGCC TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TCCGGTGTGA GAGGAGGGAA CCCAAGGTGG AGGTCATTGA GCTGCAGGAC
    GTGGAATGTG AGGAGAGACG CCACGGCAGC AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39601
    Clone ID Related Accession (Same CDS sequence) NM_001205879.2 , NM_001205879.1
    Accession Version NM_001205879.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product protein patched homolog 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02024005.1. On Apr 20, 2011 this sequence version replaced XM_599250.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822089, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGCTTGCC TATGCCTGTT TAACCATGCT GCGCTGGGAC
    TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG
    GCTGCAGGAT TGGGCCTGTG CTCGCTGATC GGGATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TCTTTCTTCT GGCGCATGCA
    TTTAGTGAAA CGGGACAGAA TAAAAGAATT CCTTTTGAGG ACAGGACCGG GGAGTGCCTC
    AAGCGCACGG GAGCCAGTGT GGCCCTCACC TCCATCAGCA ACGTCACAGC CTTCTTCATG
    GCCGCGTTGA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG
    GTGTTCAATT TTGCTATGGT TCTGCTGATT TTTCCTGCAA TTCTCAGCAT GGATTTGTAT
    CGACGTGAGG ACAGGAGATT GGATATTTTC TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA
    GTGATTCAGG TTGAACCCCA GGCCTACACA GAGACTCACG ATAACACCCG CTACAGCCCC
    CCACCCCCCT ACAGCAGCCA CGGCTTCGCC CATGAAACCC AGATCACCAT GCAGTCCACG
    GTACAGCTTC GCACAGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC TGCCGAGCCA
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC ATGGCTCAGG ACACACTCAG CTGCCAGAGC
    CCCGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCGGACTC CAGCCTCCAC
    TGCCTGGAGC CCCCCTGCAC CAGGTGGACA CTTTCATCTT TCGCTGAGAA GCACTATGCT
    CCTTTCCTCT TAAAACCAAA AGCCAAGGTC GTGGTGATCT TCCTTTTCCT GGGCCTGCTG
    GGGGTCAGCC TTTATGGGAC CACCCGAGTG CGTGATGGGC TGGATCTTAC AGACATTGTG
    CCTCGGGAAA CTAGAGAATA TGACTTTATT GCTGCACAGT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACTCA GAAAGCAGAT TACCCGAATA TCCAGCACTT ACTTTATGAC
    CTTCATAAGA GTTTCAGTAA TGTGAAGTAT GTCATGCTGG AAGAAAATAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTCCAAGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAAA ATGGATCGGA TGATGGGGTC
    CTTGCCTACA AACTCCTGGT ACAAACCGGC AGCCGTGATA AGCCCATTGA CATCAGTCAG
    CTGACTAAAC GGCGCCTGGT GGATGCAGAC GGCATCATTA ACCCCAGTGC TTTCTACATC
    TACCTGACTG CTTGGGTCAG CAACGACCCT GTGGCTTATG CCGCCTCCCA AGCCAACATC
    CGGCCTCACC GTCCTGAGTG GGTTCATGAC AAAGCCGACT ACATGCCAGA AACCAGGCTG
    AGAATCCCCG CGGCAGAGCC CATTGAATAT GCTCAGTTCC CTTTCTACCT CAACGGCCTG
    CGTGACACCT CGGACTTCGT TGAAGCCATC GAAAAAGTCA GAACCATCTG CAATAACTAC
    ACGAGCCTGG GGCTCTCCAG CTACCCCAAT GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATTGGCCTCC GCCACTGGCT CCTCCTGTCC ATCAGCGTGG TGCTGGCCTG CACGTTCCTC
    GTGTGCGCCG TCTTCCTCCT GAACCCCTGG ACGGCCGGGA TCATTGTGAC AGTCTTGGCT
    TTGATGACAG TTGAGCTCTT TGGCATGATG GGCCTCATTG GAATCAAGCT GAGTGCCGTG
    CCTGTGGTCA TCTTGATTGC TTCCGTCGGC ATTGGAGTGG AATTCACTGT TCATGTCGCT
    CTGGCCTTTC TAACAGCCAT TGGTGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT TCCACTCTAC TGGGAGTGCT GATGCTTGCA
    GGATCTGAAT TTGATTTCAT TGTCAGGTAC TTCTTTGCTG TCCTGGCAAT CCTAACAATC
    CTGGGTGTTC TCAATGGACT AGTTTTGCTG CCAGTCCTTT TGTCCTTCTT CGGACCGTAT
    CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC CGGCTGCCCA CACCTTCTCC TGAGCCACCC
    CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT GGTCACACAA ACAACGGGTC TGATTCCTCT
    GACTCGGAGT ACAGTTCTCA GACGACCGTG TCAGGCATCA GTGAGGAGCT GCGGCACTAC
    GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT CCTGAACCGA GGCATCACCC ACCCTCAAAT
    CCTCGACAGC AGTCCCACCT GGACTCAAGG ACCCTGCCAC CTGGACAGCC AGGACAGCAG
    CCCAGAAGAG AAACCCCCAG AGAATGCTTG AGGGCGCCTC CATATAAACC GCGCAGAGAC
    GCTTTCGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGACCG CACGGGCCCC
    CGGGGAGCCC GTTCTCACAA CCCTCGGCAT CACAACCCAG CGTTCACTGC CATGGGCAGC
    TCCATGCCCA GCTACTGCCA GCCCATTACC ACCGTGACGG CCTCGGCGTC CGTGACTGTT
    GCCGTGCATC CTCCGCCCGC CCCTGGGCCT GGGCCCAGTA GGAACCCCCG AAGCGGGCTT
    TGCCCAGGCT ACGAGGACTA CCCCGAGACT GACCACGGCC TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TCCGGTGTGA GAGGAGGGAA CCCAAGGTGG AGGTCATTGA GCTGCAGGAC
    GTGGAATGTG AGGAGAGACG CCACGGCAGC AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192808.1
    CDS148..4509
    Translation

    Target ORF information:

    RefSeq Version NM_001205879.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGCTTGCC TATGCCTGTT TAACCATGCT GCGCTGGGAC
    TGCTCCAGGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG
    GCTGCAGGAT TGGGCCTGTG CTCGCTGATC GGGATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCGT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TCTTTCTTCT GGCGCATGCA
    TTTAGTGAAA CGGGACAGAA TAAAAGAATT CCTTTTGAGG ACAGGACCGG GGAGTGCCTC
    AAGCGCACGG GAGCCAGTGT GGCCCTCACC TCCATCAGCA ACGTCACAGC CTTCTTCATG
    GCCGCGTTGA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TGCAGGCGGC TGTAGTAGTG
    GTGTTCAATT TTGCTATGGT TCTGCTGATT TTTCCTGCAA TTCTCAGCAT GGATTTGTAT
    CGACGTGAGG ACAGGAGATT GGATATTTTC TGCTGTTTCA CAAGCCCCTG TGTCAGCAGA
    GTGATTCAGG TTGAACCCCA GGCCTACACA GAGACTCACG ATAACACCCG CTACAGCCCC
    CCACCCCCCT ACAGCAGCCA CGGCTTCGCC CATGAAACCC AGATCACCAT GCAGTCCACG
    GTACAGCTTC GCACAGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC TGCCGAGCCA
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC ATGGCTCAGG ACACACTCAG CTGCCAGAGC
    CCCGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCGGACTC CAGCCTCCAC
    TGCCTGGAGC CCCCCTGCAC CAGGTGGACA CTTTCATCTT TCGCTGAGAA GCACTATGCT
    CCTTTCCTCT TAAAACCAAA AGCCAAGGTC GTGGTGATCT TCCTTTTCCT GGGCCTGCTG
    GGGGTCAGCC TTTATGGGAC CACCCGAGTG CGTGATGGGC TGGATCTTAC AGACATTGTG
    CCTCGGGAAA CTAGAGAATA TGACTTTATT GCTGCACAGT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACTCA GAAAGCAGAT TACCCGAATA TCCAGCACTT ACTTTATGAC
    CTTCATAAGA GTTTCAGTAA TGTGAAGTAT GTCATGCTGG AAGAAAATAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTCCAAGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAAA ATGGATCGGA TGATGGGGTC
    CTTGCCTACA AACTCCTGGT ACAAACCGGC AGCCGTGATA AGCCCATTGA CATCAGTCAG
    CTGACTAAAC GGCGCCTGGT GGATGCAGAC GGCATCATTA ACCCCAGTGC TTTCTACATC
    TACCTGACTG CTTGGGTCAG CAACGACCCT GTGGCTTATG CCGCCTCCCA AGCCAACATC
    CGGCCTCACC GTCCTGAGTG GGTTCATGAC AAAGCCGACT ACATGCCAGA AACCAGGCTG
    AGAATCCCCG CGGCAGAGCC CATTGAATAT GCTCAGTTCC CTTTCTACCT CAACGGCCTG
    CGTGACACCT CGGACTTCGT TGAAGCCATC GAAAAAGTCA GAACCATCTG CAATAACTAC
    ACGAGCCTGG GGCTCTCCAG CTACCCCAAT GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATTGGCCTCC GCCACTGGCT CCTCCTGTCC ATCAGCGTGG TGCTGGCCTG CACGTTCCTC
    GTGTGCGCCG TCTTCCTCCT GAACCCCTGG ACGGCCGGGA TCATTGTGAC AGTCTTGGCT
    TTGATGACAG TTGAGCTCTT TGGCATGATG GGCCTCATTG GAATCAAGCT GAGTGCCGTG
    CCTGTGGTCA TCTTGATTGC TTCCGTCGGC ATTGGAGTGG AATTCACTGT TCATGTCGCT
    CTGGCCTTTC TAACAGCCAT TGGTGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCGC CTGTTCTCGA TGGTGCCGTT TCCACTCTAC TGGGAGTGCT GATGCTTGCA
    GGATCTGAAT TTGATTTCAT TGTCAGGTAC TTCTTTGCTG TCCTGGCAAT CCTAACAATC
    CTGGGTGTTC TCAATGGACT AGTTTTGCTG CCAGTCCTTT TGTCCTTCTT CGGACCGTAT
    CCTGAGGTGT CTCCAGCCAA TGGGCTGAAC CGGCTGCCCA CACCTTCTCC TGAGCCACCC
    CCCAGCGTGG TCCGGTTTGC TGTGCCCGCT GGTCACACAA ACAACGGGTC TGATTCCTCT
    GACTCGGAGT ACAGTTCTCA GACGACCGTG TCAGGCATCA GTGAGGAGCT GCGGCACTAC
    GAGGCCCAGC AGGGTGCTGG GGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCTGTCTTCG CCCGCTCCAC TGTGGTCCAT CCTGAACCGA GGCATCACCC ACCCTCAAAT
    CCTCGACAGC AGTCCCACCT GGACTCAAGG ACCCTGCCAC CTGGACAGCC AGGACAGCAG
    CCCAGAAGAG AAACCCCCAG AGAATGCTTG AGGGCGCCTC CATATAAACC GCGCAGAGAC
    GCTTTCGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGACCG CACGGGCCCC
    CGGGGAGCCC GTTCTCACAA CCCTCGGCAT CACAACCCAG CGTTCACTGC CATGGGCAGC
    TCCATGCCCA GCTACTGCCA GCCCATTACC ACCGTGACGG CCTCGGCGTC CGTGACTGTT
    GCCGTGCATC CTCCGCCCGC CCCTGGGCCT GGGCCCAGTA GGAACCCCCG AAGCGGGCTT
    TGCCCAGGCT ACGAGGACTA CCCCGAGACT GACCACGGCC TGTTTGAGGA CCCCCATGTG
    CCTTTTAATG TCCGGTGTGA GAGGAGGGAA CCCAAGGTGG AGGTCATTGA GCTGCAGGAC
    GTGGAATGTG AGGAGAGACG CCACGGCAGC AGCTCCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22271
    Clone ID Related Accession (Same CDS sequence) XM_005210406.3 , XM_005210406.4
    Accession Version XM_005210406.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210406.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGGTTTTG CCGTTTCTCG CTCTTGGTGT TGGTGTGGAT
    GATGTCTTTC TTCTGGCGCA TGCATTTAGT GAAACGGGAC AGAATAAAAG AATTCCTTTT
    GAGGACAGGA CCGGGGAGTG CCTCAAGCGC ACGGGAGCCA GTGTGGCCCT CACCTCCATC
    AGCAACGTCA CAGCCTTCTT CATGGCCGCG TTGATCCCAA TTCCCGCTCT GCGGGCGTTC
    TCCCTGCAGG CGGCTGTAGT AGTGGTGTTC AATTTTGCTA TGGTTCTGCT GATTTTTCCT
    GCAATTCTCA GCATGGATTT GTATCGACGT GAGGACAGGA GATTGGATAT TTTCTGCTGT
    TTCACAAGCC CCTGTGTCAG CAGAGTGATT CAGGTTGAAC CCCAGGCCTA CACAGAGACT
    CACGATAACA CCCGCTACAG CCCCCCACCC CCCTACAGCA GCCACGGCTT CGCCCATGAA
    ACCCAGATCA CCATGCAGTC CACGGTACAG CTTCGCACAG AGTACGACCC CCACACGCAC
    GTGTACTACA CCACTGCCGA GCCACGCTCC GAGATCTCTG TGCAGCCCGT CACCATGGCT
    CAGGACACAC TCAGCTGCCA GAGCCCCGAG AGCACCAGCT CCACAAGGGA CCTGCTCTCC
    CAGTTCTCGG ACTCCAGCCT CCACTGCCTG GAGCCCCCCT GCACCAGGTG GACACTTTCA
    TCTTTCGCTG AGAAGCACTA TGCTCCTTTC CTCTTAAAAC CAAAAGCCAA GGTCGTGGTG
    ATCTTCCTTT TCCTGGGCCT GCTGGGGGTC AGCCTTTATG GGACCACCCG AGTGCGTGAT
    GGGCTGGATC TTACAGACAT TGTGCCTCGG GAAACTAGAG AATATGACTT TATTGCTGCA
    CAGTTCAAAT ACTTTTCTTT CTACAACATG TATATAGTCA CTCAGAAAGC AGATTACCCG
    AATATCCAGC ACTTACTTTA TGACCTTCAT AAGAGTTTCA GTAATGTGAA GTATGTCATG
    CTGGAAGAAA ATAAACAGCT TCCCAAAATG TGGCTGCACT ACTTCAGAGA CTGGCTCCAA
    GGACTTCAGG ATGCATTTGA CAGTGACTGG GAAACCGGGA AAATCATGCC AAACAATTAC
    AAAAATGGAT CGGATGATGG GGTCCTTGCC TACAAACTCC TGGTACAAAC CGGCAGCCGT
    GATAAGCCCA TTGACATCAG TCAGCTGACT AAACGGCGCC TGGTGGATGC AGACGGCATC
    ATTAACCCCA GTGCTTTCTA CATCTACCTG ACTGCTTGGG TCAGCAACGA CCCTGTGGCT
    TATGCCGCCT CCCAAGCCAA CATCCGGCCT CACCGTCCTG AGTGGGTTCA TGACAAAGCC
    GACTACATGC CAGAAACCAG GCTGAGAATC CCCGCGGCAG AGCCCATTGA ATATGCTCAG
    TTCCCTTTCT ACCTCAACGG CCTGCGTGAC ACCTCGGACT TCGTTGAAGC CATCGAAAAA
    GTCAGAACCA TCTGCAATAA CTACACGAGC CTGGGGCTCT CCAGCTACCC CAATGGCTAC
    CCCTTCCTCT TCTGGGAGCA GTACATTGGC CTCCGCCACT GGCTCCTCCT GTCCATCAGC
    GTGGTGCTGG CCTGCACGTT CCTCGTGTGC GCCGTCTTCC TCCTGAACCC CTGGACGGCC
    GGGATCATTG TGACAGTCTT GGCTTTGATG ACAGTTGAGC TCTTTGGCAT GATGGGCCTC
    ATTGGAATCA AGCTGAGTGC CGTGCCTGTG GTCATCTTGA TTGCTTCCGT CGGCATTGGA
    GTGGAATTCA CTGTTCATGT CGCTCTGGCC TTTCTAACAG CCATTGGTGA CAAGAACCGC
    AGGGCTGTGC TTGCCCTGGA GCACATGTTT GCGCCTGTTC TCGATGGTGC CGTTTCCACT
    CTACTGGGAG TGCTGATGCT TGCAGGATCT GAATTTGATT TCATTGTCAG GTACTTCTTT
    GCTGTCCTGG CAATCCTAAC AATCCTGGGT GTTCTCAATG GACTAGTTTT GCTGCCAGTC
    CTTTTGTCCT TCTTCGGACC GTATCCTGAG GTGTCTCCAG CCAATGGGCT GAACCGGCTG
    CCCACACCTT CTCCTGAGCC ACCCCCCAGC GTGGTCCGGT TTGCTGTGCC CGCTGGTCAC
    ACAAACAACG GGTCTGATTC CTCTGACTCG GAGTACAGTT CTCAGACGAC CGTGTCAGGC
    ATCAGTGAGG AGCTGCGGCA CTACGAGGCC CAGCAGGGTG CTGGGGGCCC TGCCCACCAA
    GTGATCGTGG AAGCCACAGA AAACCCTGTC TTCGCCCGCT CCACTGTGGT CCATCCTGAA
    CCGAGGCATC ACCCACCCTC AAATCCTCGA CAGCAGTCCC ACCTGGACTC AAGGACCCTG
    CCACCTGGAC AGCCAGGACA GCAGCCCAGA AGAGAAACCC CCAGAGAATG CTTGAGGGCG
    CCTCCATATA AACCGCGCAG AGACGCTTTC GAAATTTCTA CTGAAGGGCA TTCTGGCCCT
    AGCAATAGGG ACCGCACGGG CCCCCGGGGA GCCCGTTCTC ACAACCCTCG GCATCACAAC
    CCAGCGTTCA CTGCCATGGG CAGCTCCATG CCCAGCTACT GCCAGCCCAT TACCACCGTG
    ACGGCCTCGG CGTCCGTGAC TGTTGCCGTG CATCCTCCGC CCGCCCCTGG GCCTGGGCCC
    AGTAGGAACC CCCGAAGCGG GCTTTGCCCA GGCTACGAGG ACTACCCCGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA TGTGCCTTTT AATGTCCGGT GTGAGAGGAG GGAACCCAAG
    GTGGAGGTCA TTGAGCTGCA GGACGTGGAA TGTGAGGAGA GACGCCACGG CAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210463.1
    CDS336..4541
    Translation

    Target ORF information:

    RefSeq Version XM_005210406.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210406.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGGTTTTG CCGTTTCTCG CTCTTGGTGT TGGTGTGGAT
    GATGTCTTTC TTCTGGCGCA TGCATTTAGT GAAACGGGAC AGAATAAAAG AATTCCTTTT
    GAGGACAGGA CCGGGGAGTG CCTCAAGCGC ACGGGAGCCA GTGTGGCCCT CACCTCCATC
    AGCAACGTCA CAGCCTTCTT CATGGCCGCG TTGATCCCAA TTCCCGCTCT GCGGGCGTTC
    TCCCTGCAGG CGGCTGTAGT AGTGGTGTTC AATTTTGCTA TGGTTCTGCT GATTTTTCCT
    GCAATTCTCA GCATGGATTT GTATCGACGT GAGGACAGGA GATTGGATAT TTTCTGCTGT
    TTCACAAGCC CCTGTGTCAG CAGAGTGATT CAGGTTGAAC CCCAGGCCTA CACAGAGACT
    CACGATAACA CCCGCTACAG CCCCCCACCC CCCTACAGCA GCCACGGCTT CGCCCATGAA
    ACCCAGATCA CCATGCAGTC CACGGTACAG CTTCGCACAG AGTACGACCC CCACACGCAC
    GTGTACTACA CCACTGCCGA GCCACGCTCC GAGATCTCTG TGCAGCCCGT CACCATGGCT
    CAGGACACAC TCAGCTGCCA GAGCCCCGAG AGCACCAGCT CCACAAGGGA CCTGCTCTCC
    CAGTTCTCGG ACTCCAGCCT CCACTGCCTG GAGCCCCCCT GCACCAGGTG GACACTTTCA
    TCTTTCGCTG AGAAGCACTA TGCTCCTTTC CTCTTAAAAC CAAAAGCCAA GGTCGTGGTG
    ATCTTCCTTT TCCTGGGCCT GCTGGGGGTC AGCCTTTATG GGACCACCCG AGTGCGTGAT
    GGGCTGGATC TTACAGACAT TGTGCCTCGG GAAACTAGAG AATATGACTT TATTGCTGCA
    CAGTTCAAAT ACTTTTCTTT CTACAACATG TATATAGTCA CTCAGAAAGC AGATTACCCG
    AATATCCAGC ACTTACTTTA TGACCTTCAT AAGAGTTTCA GTAATGTGAA GTATGTCATG
    CTGGAAGAAA ATAAACAGCT TCCCAAAATG TGGCTGCACT ACTTCAGAGA CTGGCTCCAA
    GGACTTCAGG ATGCATTTGA CAGTGACTGG GAAACCGGGA AAATCATGCC AAACAATTAC
    AAAAATGGAT CGGATGATGG GGTCCTTGCC TACAAACTCC TGGTACAAAC CGGCAGCCGT
    GATAAGCCCA TTGACATCAG TCAGCTGACT AAACGGCGCC TGGTGGATGC AGACGGCATC
    ATTAACCCCA GTGCTTTCTA CATCTACCTG ACTGCTTGGG TCAGCAACGA CCCTGTGGCT
    TATGCCGCCT CCCAAGCCAA CATCCGGCCT CACCGTCCTG AGTGGGTTCA TGACAAAGCC
    GACTACATGC CAGAAACCAG GCTGAGAATC CCCGCGGCAG AGCCCATTGA ATATGCTCAG
    TTCCCTTTCT ACCTCAACGG CCTGCGTGAC ACCTCGGACT TCGTTGAAGC CATCGAAAAA
    GTCAGAACCA TCTGCAATAA CTACACGAGC CTGGGGCTCT CCAGCTACCC CAATGGCTAC
    CCCTTCCTCT TCTGGGAGCA GTACATTGGC CTCCGCCACT GGCTCCTCCT GTCCATCAGC
    GTGGTGCTGG CCTGCACGTT CCTCGTGTGC GCCGTCTTCC TCCTGAACCC CTGGACGGCC
    GGGATCATTG TGACAGTCTT GGCTTTGATG ACAGTTGAGC TCTTTGGCAT GATGGGCCTC
    ATTGGAATCA AGCTGAGTGC CGTGCCTGTG GTCATCTTGA TTGCTTCCGT CGGCATTGGA
    GTGGAATTCA CTGTTCATGT CGCTCTGGCC TTTCTAACAG CCATTGGTGA CAAGAACCGC
    AGGGCTGTGC TTGCCCTGGA GCACATGTTT GCGCCTGTTC TCGATGGTGC CGTTTCCACT
    CTACTGGGAG TGCTGATGCT TGCAGGATCT GAATTTGATT TCATTGTCAG GTACTTCTTT
    GCTGTCCTGG CAATCCTAAC AATCCTGGGT GTTCTCAATG GACTAGTTTT GCTGCCAGTC
    CTTTTGTCCT TCTTCGGACC GTATCCTGAG GTGTCTCCAG CCAATGGGCT GAACCGGCTG
    CCCACACCTT CTCCTGAGCC ACCCCCCAGC GTGGTCCGGT TTGCTGTGCC CGCTGGTCAC
    ACAAACAACG GGTCTGATTC CTCTGACTCG GAGTACAGTT CTCAGACGAC CGTGTCAGGC
    ATCAGTGAGG AGCTGCGGCA CTACGAGGCC CAGCAGGGTG CTGGGGGCCC TGCCCACCAA
    GTGATCGTGG AAGCCACAGA AAACCCTGTC TTCGCCCGCT CCACTGTGGT CCATCCTGAA
    CCGAGGCATC ACCCACCCTC AAATCCTCGA CAGCAGTCCC ACCTGGACTC AAGGACCCTG
    CCACCTGGAC AGCCAGGACA GCAGCCCAGA AGAGAAACCC CCAGAGAATG CTTGAGGGCG
    CCTCCATATA AACCGCGCAG AGACGCTTTC GAAATTTCTA CTGAAGGGCA TTCTGGCCCT
    AGCAATAGGG ACCGCACGGG CCCCCGGGGA GCCCGTTCTC ACAACCCTCG GCATCACAAC
    CCAGCGTTCA CTGCCATGGG CAGCTCCATG CCCAGCTACT GCCAGCCCAT TACCACCGTG
    ACGGCCTCGG CGTCCGTGAC TGTTGCCGTG CATCCTCCGC CCGCCCCTGG GCCTGGGCCC
    AGTAGGAACC CCCGAAGCGG GCTTTGCCCA GGCTACGAGG ACTACCCCGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA TGTGCCTTTT AATGTCCGGT GTGAGAGGAG GGAACCCAAG
    GTGGAGGTCA TTGAGCTGCA GGACGTGGAA TGTGAGGAGA GACGCCACGG CAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22271
    Clone ID Related Accession (Same CDS sequence) XM_005210406.3 , XM_005210406.4
    Accession Version XM_005210406.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210406.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGGTTTTG CCGTTTCTCG CTCTTGGTGT TGGTGTGGAT
    GATGTCTTTC TTCTGGCGCA TGCATTTAGT GAAACGGGAC AGAATAAAAG AATTCCTTTT
    GAGGACAGGA CCGGGGAGTG CCTCAAGCGC ACGGGAGCCA GTGTGGCCCT CACCTCCATC
    AGCAACGTCA CAGCCTTCTT CATGGCCGCG TTGATCCCAA TTCCCGCTCT GCGGGCGTTC
    TCCCTGCAGG CGGCTGTAGT AGTGGTGTTC AATTTTGCTA TGGTTCTGCT GATTTTTCCT
    GCAATTCTCA GCATGGATTT GTATCGACGT GAGGACAGGA GATTGGATAT TTTCTGCTGT
    TTCACAAGCC CCTGTGTCAG CAGAGTGATT CAGGTTGAAC CCCAGGCCTA CACAGAGACT
    CACGATAACA CCCGCTACAG CCCCCCACCC CCCTACAGCA GCCACGGCTT CGCCCATGAA
    ACCCAGATCA CCATGCAGTC CACGGTACAG CTTCGCACAG AGTACGACCC CCACACGCAC
    GTGTACTACA CCACTGCCGA GCCACGCTCC GAGATCTCTG TGCAGCCCGT CACCATGGCT
    CAGGACACAC TCAGCTGCCA GAGCCCCGAG AGCACCAGCT CCACAAGGGA CCTGCTCTCC
    CAGTTCTCGG ACTCCAGCCT CCACTGCCTG GAGCCCCCCT GCACCAGGTG GACACTTTCA
    TCTTTCGCTG AGAAGCACTA TGCTCCTTTC CTCTTAAAAC CAAAAGCCAA GGTCGTGGTG
    ATCTTCCTTT TCCTGGGCCT GCTGGGGGTC AGCCTTTATG GGACCACCCG AGTGCGTGAT
    GGGCTGGATC TTACAGACAT TGTGCCTCGG GAAACTAGAG AATATGACTT TATTGCTGCA
    CAGTTCAAAT ACTTTTCTTT CTACAACATG TATATAGTCA CTCAGAAAGC AGATTACCCG
    AATATCCAGC ACTTACTTTA TGACCTTCAT AAGAGTTTCA GTAATGTGAA GTATGTCATG
    CTGGAAGAAA ATAAACAGCT TCCCAAAATG TGGCTGCACT ACTTCAGAGA CTGGCTCCAA
    GGACTTCAGG ATGCATTTGA CAGTGACTGG GAAACCGGGA AAATCATGCC AAACAATTAC
    AAAAATGGAT CGGATGATGG GGTCCTTGCC TACAAACTCC TGGTACAAAC CGGCAGCCGT
    GATAAGCCCA TTGACATCAG TCAGCTGACT AAACGGCGCC TGGTGGATGC AGACGGCATC
    ATTAACCCCA GTGCTTTCTA CATCTACCTG ACTGCTTGGG TCAGCAACGA CCCTGTGGCT
    TATGCCGCCT CCCAAGCCAA CATCCGGCCT CACCGTCCTG AGTGGGTTCA TGACAAAGCC
    GACTACATGC CAGAAACCAG GCTGAGAATC CCCGCGGCAG AGCCCATTGA ATATGCTCAG
    TTCCCTTTCT ACCTCAACGG CCTGCGTGAC ACCTCGGACT TCGTTGAAGC CATCGAAAAA
    GTCAGAACCA TCTGCAATAA CTACACGAGC CTGGGGCTCT CCAGCTACCC CAATGGCTAC
    CCCTTCCTCT TCTGGGAGCA GTACATTGGC CTCCGCCACT GGCTCCTCCT GTCCATCAGC
    GTGGTGCTGG CCTGCACGTT CCTCGTGTGC GCCGTCTTCC TCCTGAACCC CTGGACGGCC
    GGGATCATTG TGACAGTCTT GGCTTTGATG ACAGTTGAGC TCTTTGGCAT GATGGGCCTC
    ATTGGAATCA AGCTGAGTGC CGTGCCTGTG GTCATCTTGA TTGCTTCCGT CGGCATTGGA
    GTGGAATTCA CTGTTCATGT CGCTCTGGCC TTTCTAACAG CCATTGGTGA CAAGAACCGC
    AGGGCTGTGC TTGCCCTGGA GCACATGTTT GCGCCTGTTC TCGATGGTGC CGTTTCCACT
    CTACTGGGAG TGCTGATGCT TGCAGGATCT GAATTTGATT TCATTGTCAG GTACTTCTTT
    GCTGTCCTGG CAATCCTAAC AATCCTGGGT GTTCTCAATG GACTAGTTTT GCTGCCAGTC
    CTTTTGTCCT TCTTCGGACC GTATCCTGAG GTGTCTCCAG CCAATGGGCT GAACCGGCTG
    CCCACACCTT CTCCTGAGCC ACCCCCCAGC GTGGTCCGGT TTGCTGTGCC CGCTGGTCAC
    ACAAACAACG GGTCTGATTC CTCTGACTCG GAGTACAGTT CTCAGACGAC CGTGTCAGGC
    ATCAGTGAGG AGCTGCGGCA CTACGAGGCC CAGCAGGGTG CTGGGGGCCC TGCCCACCAA
    GTGATCGTGG AAGCCACAGA AAACCCTGTC TTCGCCCGCT CCACTGTGGT CCATCCTGAA
    CCGAGGCATC ACCCACCCTC AAATCCTCGA CAGCAGTCCC ACCTGGACTC AAGGACCCTG
    CCACCTGGAC AGCCAGGACA GCAGCCCAGA AGAGAAACCC CCAGAGAATG CTTGAGGGCG
    CCTCCATATA AACCGCGCAG AGACGCTTTC GAAATTTCTA CTGAAGGGCA TTCTGGCCCT
    AGCAATAGGG ACCGCACGGG CCCCCGGGGA GCCCGTTCTC ACAACCCTCG GCATCACAAC
    CCAGCGTTCA CTGCCATGGG CAGCTCCATG CCCAGCTACT GCCAGCCCAT TACCACCGTG
    ACGGCCTCGG CGTCCGTGAC TGTTGCCGTG CATCCTCCGC CCGCCCCTGG GCCTGGGCCC
    AGTAGGAACC CCCGAAGCGG GCTTTGCCCA GGCTACGAGG ACTACCCCGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA TGTGCCTTTT AATGTCCGGT GTGAGAGGAG GGAACCCAAG
    GTGGAGGTCA TTGAGCTGCA GGACGTGGAA TGTGAGGAGA GACGCCACGG CAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210463.1
    CDS1..4206
    Translation

    Target ORF information:

    RefSeq Version XM_005210406.4
    Organism Bos taurus(cattle)
    Definition Bos taurus patched 1 (PTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210406.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCTCGG CTGGTAACGC CGCCGAGCTC CAGAACCGCG GCGGCGGCGG CAGCTGCAGC 
    GGGGCCCCGG GCCGGCCGGC CGGTGGCGGG AGGCGCAGGC GGACCGGGGG ACTGCGCCGC
    AACGCCGTGC CGGACTGGGA CTATCTGCAC CGGCCCAGCT ACTGCGACGC CGCCTTCGCT
    CTGGAGCAGA TTTCCAAGGG GAAAGCTACT GGCCGGAAAG CGCCGCTGTG GCTGCGAGCG
    AAGTTTCAAA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTAG GCCTCCTCAT ATTTGGGGCC TTCGCTGTGG GATTAAAGGC AGCTAATCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGTGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATTCAGACC
    CCCAAGGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCGACAGCA CCTGGACTCG
    GCGCTCCAGG CCAGCCGGGT CCATGTATAC ATGTATAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTATA AATCTGGAGA ACTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAG
    TATCTTTACC CTTGTTTAAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAGTTA
    CAGTCTGGGA CGGCATACCT ACTAGGTAAG CCTCCTTTGC AGTGGACAAA CTTTGACCCT
    TTGGAATTCC TAGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAAGTTGG TCATGGTTAC ATGGACCGGC CCTGCCTCAA TCCAGCTGAT
    CCAGACTGTC CCGCCACAGC CCCCAACAAA AATGCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGGTGTCA TGGATTATCC AGAAAGTATA TGCACTGGCA GGAGGAACTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGGAAGCTTG TCAGTGCCCA TGCTCTGCAG
    ACCATGTTTC AGTTAATGAC TCCCAAGCAA ATGTATGAAC ACTTCAAGGG TTATGAGTAT
    GTCTCGCACA TCAACTGGAA CGAGGACAAG GCAGCAGCCA TCCTGGAGGC TTGGCAAAGG
    ACGTACGTGG AGGTGGTTCA TCAGAGTGTT GCTCAGAACT CCACTCAGAA GGTGCTTTCC
    TTTACCACGA CGACCCTGGA TGACATCCTC AAGTCCTTCT CTGATGTCAG CGTCATCCGA
    GTGGCCAGCG GCTATTTACT GATGGTTTTG CCGTTTCTCG CTCTTGGTGT TGGTGTGGAT
    GATGTCTTTC TTCTGGCGCA TGCATTTAGT GAAACGGGAC AGAATAAAAG AATTCCTTTT
    GAGGACAGGA CCGGGGAGTG CCTCAAGCGC ACGGGAGCCA GTGTGGCCCT CACCTCCATC
    AGCAACGTCA CAGCCTTCTT CATGGCCGCG TTGATCCCAA TTCCCGCTCT GCGGGCGTTC
    TCCCTGCAGG CGGCTGTAGT AGTGGTGTTC AATTTTGCTA TGGTTCTGCT GATTTTTCCT
    GCAATTCTCA GCATGGATTT GTATCGACGT GAGGACAGGA GATTGGATAT TTTCTGCTGT
    TTCACAAGCC CCTGTGTCAG CAGAGTGATT CAGGTTGAAC CCCAGGCCTA CACAGAGACT
    CACGATAACA CCCGCTACAG CCCCCCACCC CCCTACAGCA GCCACGGCTT CGCCCATGAA
    ACCCAGATCA CCATGCAGTC CACGGTACAG CTTCGCACAG AGTACGACCC CCACACGCAC
    GTGTACTACA CCACTGCCGA GCCACGCTCC GAGATCTCTG TGCAGCCCGT CACCATGGCT
    CAGGACACAC TCAGCTGCCA GAGCCCCGAG AGCACCAGCT CCACAAGGGA CCTGCTCTCC
    CAGTTCTCGG ACTCCAGCCT CCACTGCCTG GAGCCCCCCT GCACCAGGTG GACACTTTCA
    TCTTTCGCTG AGAAGCACTA TGCTCCTTTC CTCTTAAAAC CAAAAGCCAA GGTCGTGGTG
    ATCTTCCTTT TCCTGGGCCT GCTGGGGGTC AGCCTTTATG GGACCACCCG AGTGCGTGAT
    GGGCTGGATC TTACAGACAT TGTGCCTCGG GAAACTAGAG AATATGACTT TATTGCTGCA
    CAGTTCAAAT ACTTTTCTTT CTACAACATG TATATAGTCA CTCAGAAAGC AGATTACCCG
    AATATCCAGC ACTTACTTTA TGACCTTCAT AAGAGTTTCA GTAATGTGAA GTATGTCATG
    CTGGAAGAAA ATAAACAGCT TCCCAAAATG TGGCTGCACT ACTTCAGAGA CTGGCTCCAA
    GGACTTCAGG ATGCATTTGA CAGTGACTGG GAAACCGGGA AAATCATGCC AAACAATTAC
    AAAAATGGAT CGGATGATGG GGTCCTTGCC TACAAACTCC TGGTACAAAC CGGCAGCCGT
    GATAAGCCCA TTGACATCAG TCAGCTGACT AAACGGCGCC TGGTGGATGC AGACGGCATC
    ATTAACCCCA GTGCTTTCTA CATCTACCTG ACTGCTTGGG TCAGCAACGA CCCTGTGGCT
    TATGCCGCCT CCCAAGCCAA CATCCGGCCT CACCGTCCTG AGTGGGTTCA TGACAAAGCC
    GACTACATGC CAGAAACCAG GCTGAGAATC CCCGCGGCAG AGCCCATTGA ATATGCTCAG
    TTCCCTTTCT ACCTCAACGG CCTGCGTGAC ACCTCGGACT TCGTTGAAGC CATCGAAAAA
    GTCAGAACCA TCTGCAATAA CTACACGAGC CTGGGGCTCT CCAGCTACCC CAATGGCTAC
    CCCTTCCTCT TCTGGGAGCA GTACATTGGC CTCCGCCACT GGCTCCTCCT GTCCATCAGC
    GTGGTGCTGG CCTGCACGTT CCTCGTGTGC GCCGTCTTCC TCCTGAACCC CTGGACGGCC
    GGGATCATTG TGACAGTCTT GGCTTTGATG ACAGTTGAGC TCTTTGGCAT GATGGGCCTC
    ATTGGAATCA AGCTGAGTGC CGTGCCTGTG GTCATCTTGA TTGCTTCCGT CGGCATTGGA
    GTGGAATTCA CTGTTCATGT CGCTCTGGCC TTTCTAACAG CCATTGGTGA CAAGAACCGC
    AGGGCTGTGC TTGCCCTGGA GCACATGTTT GCGCCTGTTC TCGATGGTGC CGTTTCCACT
    CTACTGGGAG TGCTGATGCT TGCAGGATCT GAATTTGATT TCATTGTCAG GTACTTCTTT
    GCTGTCCTGG CAATCCTAAC AATCCTGGGT GTTCTCAATG GACTAGTTTT GCTGCCAGTC
    CTTTTGTCCT TCTTCGGACC GTATCCTGAG GTGTCTCCAG CCAATGGGCT GAACCGGCTG
    CCCACACCTT CTCCTGAGCC ACCCCCCAGC GTGGTCCGGT TTGCTGTGCC CGCTGGTCAC
    ACAAACAACG GGTCTGATTC CTCTGACTCG GAGTACAGTT CTCAGACGAC CGTGTCAGGC
    ATCAGTGAGG AGCTGCGGCA CTACGAGGCC CAGCAGGGTG CTGGGGGCCC TGCCCACCAA
    GTGATCGTGG AAGCCACAGA AAACCCTGTC TTCGCCCGCT CCACTGTGGT CCATCCTGAA
    CCGAGGCATC ACCCACCCTC AAATCCTCGA CAGCAGTCCC ACCTGGACTC AAGGACCCTG
    CCACCTGGAC AGCCAGGACA GCAGCCCAGA AGAGAAACCC CCAGAGAATG CTTGAGGGCG
    CCTCCATATA AACCGCGCAG AGACGCTTTC GAAATTTCTA CTGAAGGGCA TTCTGGCCCT
    AGCAATAGGG ACCGCACGGG CCCCCGGGGA GCCCGTTCTC ACAACCCTCG GCATCACAAC
    CCAGCGTTCA CTGCCATGGG CAGCTCCATG CCCAGCTACT GCCAGCCCAT TACCACCGTG
    ACGGCCTCGG CGTCCGTGAC TGTTGCCGTG CATCCTCCGC CCGCCCCTGG GCCTGGGCCC
    AGTAGGAACC CCCGAAGCGG GCTTTGCCCA GGCTACGAGG ACTACCCCGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA TGTGCCTTTT AATGTCCGGT GTGAGAGGAG GGAACCCAAG
    GTGGAGGTCA TTGAGCTGCA GGACGTGGAA TGTGAGGAGA GACGCCACGG CAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.