BTBD8 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010803304.2	XP_010801606.1	BTB/POZ domain-containing protein 8 isoform X1
XM_024989022.1	XP_024844790.1	BTB/POZ domain-containing protein 8 isoform X3
XM_024989037.1	XP_024844805.1	BTB/POZ domain-containing protein 8 isoform X4
XM_010803309.3	XP_010801611.1	BTB/POZ domain-containing protein 8 isoform X5
XM_010803304.1	XP_010801606.1	BTB/POZ domain-containing protein 8 isoform X1
XM_010803305.1	XP_010801607.1	BTB/POZ domain-containing protein 8 isoform X2
XM_024989033.1	XP_024844801.1	BTB/POZ domain-containing protein 8 isoform X4
XM_024989050.1	XP_024844818.1	BTB/POZ domain-containing protein 8 isoform X6
XM_024989028.1	XP_024844796.1	BTB/POZ domain-containing protein 8 isoform X4
XM_010803308.3	XP_010801610.1	BTB/POZ domain-containing protein 8 isoform X4
NM_001105200.1	NP_001098670.1	BTB/POZ domain-containing protein 8
XM_010803308.2	XP_010801610.1	BTB/POZ domain-containing protein 8 isoform X3
XM_010803309.2	XP_010801611.1	BTB/POZ domain-containing protein 8 isoform X4
XM_010803305.2	XP_010801607.1	BTB/POZ domain-containing protein 8 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37312	NM_001105200.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa232763	XM_024989050.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa50258	XM_024989022.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa50256	XM_010803304.2 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa50257	XM_010803305.2 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa50259	XM_024989033.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50259	XM_010803308.3 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50259	XM_024989037.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50259	XM_024989028.1 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50260	XM_010803309.3 Latest version!	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa50256	XM_010803304.1	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa50259	XM_010803308.2	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50260	XM_010803309.2	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50257	XM_010803305.1	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

BTBD8 ( NM_001105200.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098670.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), mRNA.

Price & Availability↑

CloneID	OBa37312
Clone ID Related Accession (Same CDS sequence)	NM_001105200.1
Accession Version	NM_001105200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On Sep 20, 2007 this sequence version replaced XM_598700.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAGT AAGTATAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098670.1
CDS	1..1173
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRLLREEVHTDVTFSIGCTLFKAHKAVLLARVPDFYFHTVGHTDNLKNHEHVTVENFEASEFRTFLQIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLVSIT

Target ORF information:

RefSeq Version	NM_001105200.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105200.1

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAGT AAGTATAACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_024989050.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844818.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa232763
Clone ID Related Accession (Same CDS sequence)	XM_024989050.1
Accession Version	XM_024989050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC AGCTCCAAGA AGAATGCATT GCATTTATTA TAGAAAATTT CTCCAAGATC 
ATTCAAAGTG AAAATTTTGC TCTTCTGTTA CAGTCACAAG CAATGAGTAG CACATCTGAT 
CTGTTGGACA AAATTTTTAA AGCTGTTGAA GAAAACATTA CCACTGAGAA TAGTTGCTCG 
CTTCTTATGG CTCTGGACAC ATTACTGAAC TCTGACAGTA CAAAAGAAAT GGGTTTTACG 
TGCAAGATCC AGGCTCTGCG TGATAAGCTG TGGATCTTCC TGGTTCAGTC TTTCTATGCT 
GTTCGTCACA CAGAAAGCTG GAAGCTGATG AGCACAGATG ATCAACAGAA AATCCAAGCA 
GCTGCATTTG ACAAAGGTGA TGACCGAAGA CTTGGCAAAA AGCCTATATT CAGTAGCTCT 
CAGCAAAGAA AACAGGTTTC TGACTGTGGT GCTATAAAAA ACAAATCCGC TGGAGGAAGT 
AACAAGAAAC AGTGTCTGAG TTATCTCTCT GCTCATCAGA AGATGAAATC TGATGGATTA 
GGAGCCTCTG GACATTCATC AAGTACTAAT AGAAATTCTA TAAATAAAAC TTTGAAGCAT 
GATGATTTAA AGGAAAAGGA TCCAAAACCA GCATCCAAGA GTACAAAAGA ATTAAAAACT 
GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA GCTATCATAA AGCCCAAAAC AGAAAATAGT 
GATAATGCAA AGTCAACAAA CCTGTCACCT AGACAAGCTG TGGAAAGATC AGCAACAGCA 
GCAGCAAATG AACAGAAAAA TTCAGGAAAT GGAAAAGGAG TGAGAAATCA GGAAGAGCAA 
ATTACAGGTG CCAGACCCAA GGTACTCACT GGAAATTTAA ATGTGCAAGC CAAAGCAAAG 
CCTTTGAAGA AAGTGACAGG CAAAGATTCT CCACACCTCC GAATGGCAGG GCCCTCCAGC 
AGATCTACAA ATTCAAGTAT GGAATTACTG ACTTCCACTG ACTCTCTAGA TGAACCAAAA 
GAAAATGGGT CAATGAAAGA GAAGCTTCCT GGTCATAAAC CTTCCTCTTG TGACTCTCGA 
GGACAGACTG TGAAAAACAG TGTAGAAGGT ATCAAAACTT CCACTGTAGC AATGAAATAT 
CGACCTGTTT CAAAAGATAC CAATGGAATT TCCAATAAAA AAAGCACTCA TGAACAGGAA 
TCTAATACAC ATAACAGTGT GCTAAAGAAG ATCACCAGCA AAGGATACAG TGATCCAGTA 
CCACAGGCAA TTTTAAAAAA AAGGGGAAAT GGCAATGGAT GTGCTACAGC TCCGCAGAGG 
ACAAAGAATG CCACAGCTAA TCTTGCTAAA ACTCAAGGAT CCCAAGGATC ACCAAATTCA 
GTAAAATCTT CAGTCTCTTC AAGGCAATCT GATGAAAATG TGACAAAATT GGACCACAGT 
GTAACTACAG ATAAACAGAC GCCTAAGAGA AAAATTGTCA AGCAAGGACA AACAACTTTG 
CCTAAGATTG GTGCAAAAAA GGTCACAATG CCTAAAAACC CAAATCAACC CAAAAGAGGT 
GAAAATTTGA ATAATAAAGA TGCAAAACCG AAAACACTTC CTGAACAGGT TATACTGAAG 
ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA AGTGAACCAT CTGTTGTCCA AAAAAGTGTG 
TCTCTTGATG TGTGTGACAA TAATAACAAA GGCAGTGTTT CTGAACAGAA GCCTCATGAA 
CCTCTAACTA ATCTCACAGC CAAAACCAGT GATGCAGAAG CATTTCAGTC ACCATGCATA 
CTCGACTCAC AGAAGACACT GAACAATCAA GAAAAACAGA AGTTGGTGTT AGAATGCCAA 
AATATTTCAA ATCTGGATAA ATTAATAAAA CGTGAACTGG AATCAAAACA GATCTGTTCA 
GTTAAAAGTG AAACAAATGT TTCTGGCCAC AAAAAAATAG ATCACTGCAC CACAGCTAAA 
ATACCTTGTC ATTCTGATGA GACTGATAAT GTCGATCCAA AATTTTATAG TACCACTGCT 
CTAAAATCCA TGATTTCAAA TCCTAAAGAA AACTCTTTGA ACTCTAATCC AGTTTGTGAC 
TTAGACTCAG CACACGTAGA ACAACTCCAT TCAGAATTAG ATAGGAAGAA GCAAGTAGGG 
AGAAAAGATA CAAACCAAAA ATTAAGCATT AAATGTGTGA AAGATGTTTT ACCTTGGGTT 
CCTGAAAAGA CAAATGGTAC CTTAAATTCT GGTCAAGATG ACAGAAAATC TAAAATTCAT 
GTAGAAGAAC AGACAGTTCC TAGTCAGTTG TCTGATGACT CTGCCATGAA TGAAGACAAT 
CATGCTACAG TAGAGTCACA TATTTCCTCC AAGTGTTTTT TGGAACAAAT ATCAGGGAAA 
AATTCTAAAG ATACGGAAAC AACAGAAACT TCAGAGAGCC ACGAAACTCC AGAAGCACCA 
TTCATGAGTC ACTGGAATTT GAGTACCAAT GTTCTGCATC AGAGAGAGAG TCCTGAATCA 
GACAGTGGCA GTGTCACAAC ATCCTCTGAT GACATAAAGC CCAGATCTGA AGACTATGAT 
GCTGGAGGGT CTCAGGATGA TGATGGGTCA AATGACAGAG GGGTTTCTAA ATGTGGCACT 
ATGCTGTGCC ATGATTTTCT TGGAAGGAGC AGCAGTGATA CCAGTACTCC TGAAGAATTA 
AAAATATATG ATAGCAACTT AAGAATTGAA GTGAAAATGA AAAAGCAAGG TAGTAGTGAT 
CTTTTCCAAG TTAATTCAAC AAGTGATGAT GAGATTCCCA GGAAAAGGCC AGAAATTTGG 
TCTCGATCTA CAATAGTTCA TCCTAGGGAA AAAGAAAATA TTCCACGAGG CAGTGTCCAG 
TTTGTACAGG AGGTAGATCA GGTTTCTTCC TCAGCAGATG AAACAGAAGA TGAAAGGTCT 
GAAGCTGAAA ATGTTGCAGA AAATTTCTCT ACATCTAACC CAGCTCTTCA GCAGTTTCAG 
GGGATAATTA ATCTAGCTTT TGAAGATGCA ACTGAAAATG AAAGTCATGA GTTTCGTGTA 
ACTAAAAGTT TTAAAAGGTC TGTTTTACTT TCCGTAGATG AATGTGAAGA GATAGGATCT 
GATGAAGGGG AGGTCCATGC TCTCTTTCAA CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT 
GTTTTTGATG GCATTTCTCA TGAACATCAT GGAAGGACCT GCTACTCCAG ATACTCACAT 
GGAAGTGAAG GTGGTGTTTT AGAATGCAGA CAAAAGAAAA GCAATACTAT ATATAAAAAC 
AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT GACTCATCAA GAAAAGATAA ACAGAGTGCT 
TCAGCCACAG AAAAAAAGGG TACAACAGAT GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT 
CCAGGAGATA AAGTATGTAG TGGAAGCAAT GTGGATAATG ACTTTGAGAC ACACAGCAAA 
TTTTCAGATA GTGATATAAA ATCTCAAGAA AGACCATGTC ATTTGGAACT TCGTCAAAGA 
GAATCCAATT CCGACATACC AAAGAACAGC TTCACAAAGT CTCTGGACTC CTGTCGGAGT 
CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA GAAAGCCATT CTACAGCTAC CGAAAAAGCT 
AATATTGCTT TATCTGCAGG AGACATAGAT GGTTGTGACA CAGTGGCACA AATCTACATG 
TATGACCATC GGCCTTCAAA AACCCTGTCT CCAATATATG AGATGGATGT AACAGAAGCA 
TTTGAGCAGA AAATGGAATC AGAAACACAA GGTACAGACA TGGATTTTGA AGATGAGCAA 
CACTTTGCAA AACAAGACTG GACACTATTA AAGCAACTGC TCTCTGAACA GGACTCTAAC 
TTAAATATCA CAAATTCTGT TCCTGAAGAC TTAAATTTAG CACAGTATCT AATTAATCAG 
ACACTACTTT TAGCACGAGA CAGCTCAAAA CCTCAGGGTA AAGCACATGT AGACACTTTG 
AGCAGATGGA GTGAACTAAC ATCTCCATTT GATGATTCCT CAGCAAGCAT TACCATGGCT 
AGTTTTTCCT CTGAAGAATG TTCACCCCAA GGGGAATGGA CAATTCTGGA ACTGGAAACT 
CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844818.1
CDS	159..4307
Translation	MASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_024989050.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989050.1

ORF Insert Sequence:

ATGGCATCTC AGCTCCAAGA AGAATGCATT GCATTTATTA TAGAAAATTT CTCCAAGATC 
ATTCAAAGTG AAAATTTTGC TCTTCTGTTA CAGTCACAAG CAATGAGTAG CACATCTGAT 
CTGTTGGACA AAATTTTTAA AGCTGTTGAA GAAAACATTA CCACTGAGAA TAGTTGCTCG 
CTTCTTATGG CTCTGGACAC ATTACTGAAC TCTGACAGTA CAAAAGAAAT GGGTTTTACG 
TGCAAGATCC AGGCTCTGCG TGATAAGCTG TGGATCTTCC TGGTTCAGTC TTTCTATGCT 
GTTCGTCACA CAGAAAGCTG GAAGCTGATG AGCACAGATG ATCAACAGAA AATCCAAGCA 
GCTGCATTTG ACAAAGGTGA TGACCGAAGA CTTGGCAAAA AGCCTATATT CAGTAGCTCT 
CAGCAAAGAA AACAGGTTTC TGACTGTGGT GCTATAAAAA ACAAATCCGC TGGAGGAAGT 
AACAAGAAAC AGTGTCTGAG TTATCTCTCT GCTCATCAGA AGATGAAATC TGATGGATTA 
GGAGCCTCTG GACATTCATC AAGTACTAAT AGAAATTCTA TAAATAAAAC TTTGAAGCAT 
GATGATTTAA AGGAAAAGGA TCCAAAACCA GCATCCAAGA GTACAAAAGA ATTAAAAACT 
GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA GCTATCATAA AGCCCAAAAC AGAAAATAGT 
GATAATGCAA AGTCAACAAA CCTGTCACCT AGACAAGCTG TGGAAAGATC AGCAACAGCA 
GCAGCAAATG AACAGAAAAA TTCAGGAAAT GGAAAAGGAG TGAGAAATCA GGAAGAGCAA 
ATTACAGGTG CCAGACCCAA GGTACTCACT GGAAATTTAA ATGTGCAAGC CAAAGCAAAG 
CCTTTGAAGA AAGTGACAGG CAAAGATTCT CCACACCTCC GAATGGCAGG GCCCTCCAGC 
AGATCTACAA ATTCAAGTAT GGAATTACTG ACTTCCACTG ACTCTCTAGA TGAACCAAAA 
GAAAATGGGT CAATGAAAGA GAAGCTTCCT GGTCATAAAC CTTCCTCTTG TGACTCTCGA 
GGACAGACTG TGAAAAACAG TGTAGAAGGT ATCAAAACTT CCACTGTAGC AATGAAATAT 
CGACCTGTTT CAAAAGATAC CAATGGAATT TCCAATAAAA AAAGCACTCA TGAACAGGAA 
TCTAATACAC ATAACAGTGT GCTAAAGAAG ATCACCAGCA AAGGATACAG TGATCCAGTA 
CCACAGGCAA TTTTAAAAAA AAGGGGAAAT GGCAATGGAT GTGCTACAGC TCCGCAGAGG 
ACAAAGAATG CCACAGCTAA TCTTGCTAAA ACTCAAGGAT CCCAAGGATC ACCAAATTCA 
GTAAAATCTT CAGTCTCTTC AAGGCAATCT GATGAAAATG TGACAAAATT GGACCACAGT 
GTAACTACAG ATAAACAGAC GCCTAAGAGA AAAATTGTCA AGCAAGGACA AACAACTTTG 
CCTAAGATTG GTGCAAAAAA GGTCACAATG CCTAAAAACC CAAATCAACC CAAAAGAGGT 
GAAAATTTGA ATAATAAAGA TGCAAAACCG AAAACACTTC CTGAACAGGT TATACTGAAG 
ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA AGTGAACCAT CTGTTGTCCA AAAAAGTGTG 
TCTCTTGATG TGTGTGACAA TAATAACAAA GGCAGTGTTT CTGAACAGAA GCCTCATGAA 
CCTCTAACTA ATCTCACAGC CAAAACCAGT GATGCAGAAG CATTTCAGTC ACCATGCATA 
CTCGACTCAC AGAAGACACT GAACAATCAA GAAAAACAGA AGTTGGTGTT AGAATGCCAA 
AATATTTCAA ATCTGGATAA ATTAATAAAA CGTGAACTGG AATCAAAACA GATCTGTTCA 
GTTAAAAGTG AAACAAATGT TTCTGGCCAC AAAAAAATAG ATCACTGCAC CACAGCTAAA 
ATACCTTGTC ATTCTGATGA GACTGATAAT GTCGATCCAA AATTTTATAG TACCACTGCT 
CTAAAATCCA TGATTTCAAA TCCTAAAGAA AACTCTTTGA ACTCTAATCC AGTTTGTGAC 
TTAGACTCAG CACACGTAGA ACAACTCCAT TCAGAATTAG ATAGGAAGAA GCAAGTAGGG 
AGAAAAGATA CAAACCAAAA ATTAAGCATT AAATGTGTGA AAGATGTTTT ACCTTGGGTT 
CCTGAAAAGA CAAATGGTAC CTTAAATTCT GGTCAAGATG ACAGAAAATC TAAAATTCAT 
GTAGAAGAAC AGACAGTTCC TAGTCAGTTG TCTGATGACT CTGCCATGAA TGAAGACAAT 
CATGCTACAG TAGAGTCACA TATTTCCTCC AAGTGTTTTT TGGAACAAAT ATCAGGGAAA 
AATTCTAAAG ATACGGAAAC AACAGAAACT TCAGAGAGCC ACGAAACTCC AGAAGCACCA 
TTCATGAGTC ACTGGAATTT GAGTACCAAT GTTCTGCATC AGAGAGAGAG TCCTGAATCA 
GACAGTGGCA GTGTCACAAC ATCCTCTGAT GACATAAAGC CCAGATCTGA AGACTATGAT 
GCTGGAGGGT CTCAGGATGA TGATGGGTCA AATGACAGAG GGGTTTCTAA ATGTGGCACT 
ATGCTGTGCC ATGATTTTCT TGGAAGGAGC AGCAGTGATA CCAGTACTCC TGAAGAATTA 
AAAATATATG ATAGCAACTT AAGAATTGAA GTGAAAATGA AAAAGCAAGG TAGTAGTGAT 
CTTTTCCAAG TTAATTCAAC AAGTGATGAT GAGATTCCCA GGAAAAGGCC AGAAATTTGG 
TCTCGATCTA CAATAGTTCA TCCTAGGGAA AAAGAAAATA TTCCACGAGG CAGTGTCCAG 
TTTGTACAGG AGGTAGATCA GGTTTCTTCC TCAGCAGATG AAACAGAAGA TGAAAGGTCT 
GAAGCTGAAA ATGTTGCAGA AAATTTCTCT ACATCTAACC CAGCTCTTCA GCAGTTTCAG 
GGGATAATTA ATCTAGCTTT TGAAGATGCA ACTGAAAATG AAAGTCATGA GTTTCGTGTA 
ACTAAAAGTT TTAAAAGGTC TGTTTTACTT TCCGTAGATG AATGTGAAGA GATAGGATCT 
GATGAAGGGG AGGTCCATGC TCTCTTTCAA CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT 
GTTTTTGATG GCATTTCTCA TGAACATCAT GGAAGGACCT GCTACTCCAG ATACTCACAT 
GGAAGTGAAG GTGGTGTTTT AGAATGCAGA CAAAAGAAAA GCAATACTAT ATATAAAAAC 
AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT GACTCATCAA GAAAAGATAA ACAGAGTGCT 
TCAGCCACAG AAAAAAAGGG TACAACAGAT GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT 
CCAGGAGATA AAGTATGTAG TGGAAGCAAT GTGGATAATG ACTTTGAGAC ACACAGCAAA 
TTTTCAGATA GTGATATAAA ATCTCAAGAA AGACCATGTC ATTTGGAACT TCGTCAAAGA 
GAATCCAATT CCGACATACC AAAGAACAGC TTCACAAAGT CTCTGGACTC CTGTCGGAGT 
CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA GAAAGCCATT CTACAGCTAC CGAAAAAGCT 
AATATTGCTT TATCTGCAGG AGACATAGAT GGTTGTGACA CAGTGGCACA AATCTACATG 
TATGACCATC GGCCTTCAAA AACCCTGTCT CCAATATATG AGATGGATGT AACAGAAGCA 
TTTGAGCAGA AAATGGAATC AGAAACACAA GGTACAGACA TGGATTTTGA AGATGAGCAA 
CACTTTGCAA AACAAGACTG GACACTATTA AAGCAACTGC TCTCTGAACA GGACTCTAAC 
TTAAATATCA CAAATTCTGT TCCTGAAGAC TTAAATTTAG CACAGTATCT AATTAATCAG 
ACACTACTTT TAGCACGAGA CAGCTCAAAA CCTCAGGGTA AAGCACATGT AGACACTTTG 
AGCAGATGGA GTGAACTAAC ATCTCCATTT GATGATTCCT CAGCAAGCAT TACCATGGCT 
AGTTTTTCCT CTGAAGAATG TTCACCCCAA GGGGAATGGA CAATTCTGGA ACTGGAAACT 
CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_024989022.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844790.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.

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CloneID	OBa50258
Clone ID Related Accession (Same CDS sequence)	XM_024989022.1
Accession Version	XM_024989022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG ATTGTATACT CATCAAACAG AAATATAAAA 
AGCTACGAAG AAGAAATTCT TAGGAAAAAG ATAGTGGAAA GTGGAGTACC ACAAAAGAAA 
TGTGACTTCA GTGTTGGAAA TTGTACTGAC CATGATAGAA GGTTATCTGT AGAGTTCTTA 
AGTAAAAGAT CATCTGATCA TTCTCTTCTG AAACATGAAA TTCCAAAAGA TATCAACAGC 
AAAGAGGAAA ATGTAATTTC CACTGATATT TATGACTTGG AACCTGCATC TGAATTAGGA 
GAAGATTTAC TGAAGCTTTA TGTGAAACAC TGTTGCCCAG ATATTGATAT TTATGTTGAC 
GGAAAAAGTT TTAAAGCTCA CAGAGCCATT TTAAGTGCCA GATCTAGTTA TTTTGCTGCA 
ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844790.1
CDS	248..5449
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_024989022.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989022.1

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG ATTGTATACT CATCAAACAG AAATATAAAA 
AGCTACGAAG AAGAAATTCT TAGGAAAAAG ATAGTGGAAA GTGGAGTACC ACAAAAGAAA 
TGTGACTTCA GTGTTGGAAA TTGTACTGAC CATGATAGAA GGTTATCTGT AGAGTTCTTA 
AGTAAAAGAT CATCTGATCA TTCTCTTCTG AAACATGAAA TTCCAAAAGA TATCAACAGC 
AAAGAGGAAA ATGTAATTTC CACTGATATT TATGACTTGG AACCTGCATC TGAATTAGGA 
GAAGATTTAC TGAAGCTTTA TGTGAAACAC TGTTGCCCAG ATATTGATAT TTATGTTGAC 
GGAAAAAGTT TTAAAGCTCA CAGAGCCATT TTAAGTGCCA GATCTAGTTA TTTTGCTGCA 
ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803304.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801606.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.

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CloneID	OBa50256
Clone ID Related Accession (Same CDS sequence)	XM_010803304.2 , XM_010803304.1
Accession Version	XM_010803304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GTCACAAGCA ATGAGTAGCA CATCTGATCT GTTGGACAAA 
ATTTTTAAAG CTGTTGAAGA AAACATTACC ACTGAGAATA GTTGCTCGCT TCTTATGGCT 
CTGGACACAT TACTGAACTC TGACAGTACA AAAGAAATGG GTTTTACGTG CAAGATCCAG 
GCTCTGCGTG ATAAGCTGTG GATCTTCCTG GTTCAGTCTT TCTATGCTGT TCGTCACACA 
GAAAGCTGGA AGCTGATGAG CACAGATGAT CAACAGAAAA TCCAAGCAGC TGCATTTGAC 
AAAGGTGATG ACCGAAGACT TGGCAAAAAG CCTATATTCA GTAGCTCTCA GCAAAGAAAA 
CAGGTTTCTG ACTGTGGTGC TATAAAAAAC AAATCCGCTG GAGGAAGTAA CAAGAAACAG 
TGTCTGAGTT ATCTCTCTGC TCATCAGAAG ATGAAATCTG ATGGATTAGG AGCCTCTGGA 
CATTCATCAA GTACTAATAG AAATTCTATA AATAAAACTT TGAAGCATGA TGATTTAAAG 
GAAAAGGATC CAAAACCAGC ATCCAAGAGT ACAAAAGAAT TAAAAACTGT GGAAAAAAAT 
GTTTCTGGAA AGCCCAAAGC TATCATAAAG CCCAAAACAG AAAATAGTGA TAATGCAAAG 
TCAACAAACC TGTCACCTAG ACAAGCTGTG GAAAGATCAG CAACAGCAGC AGCAAATGAA 
CAGAAAAATT CAGGAAATGG AAAAGGAGTG AGAAATCAGG AAGAGCAAAT TACAGGTGCC 
AGACCCAAGG TACTCACTGG AAATTTAAAT GTGCAAGCCA AAGCAAAGCC TTTGAAGAAA 
GTGACAGGCA AAGATTCTCC ACACCTCCGA ATGGCAGGGC CCTCCAGCAG ATCTACAAAT 
TCAAGTATGG AATTACTGAC TTCCACTGAC TCTCTAGATG AACCAAAAGA AAATGGGTCA 
ATGAAAGAGA AGCTTCCTGG TCATAAACCT TCCTCTTGTG ACTCTCGAGG ACAGACTGTG 
AAAAACAGTG TAGAAGGTAT CAAAACTTCC ACTGTAGCAA TGAAATATCG ACCTGTTTCA 
AAAGATACCA ATGGAATTTC CAATAAAAAA AGCACTCATG AACAGGAATC TAATACACAT 
AACAGTGTGC TAAAGAAGAT CACCAGCAAA GGATACAGTG ATCCAGTACC ACAGGCAATT 
TTAAAAAAAA GGGGAAATGG CAATGGATGT GCTACAGCTC CGCAGAGGAC AAAGAATGCC 
ACAGCTAATC TTGCTAAAAC TCAAGGATCC CAAGGATCAC CAAATTCAGT AAAATCTTCA 
GTCTCTTCAA GGCAATCTGA TGAAAATGTG ACAAAATTGG ACCACAGTGT AACTACAGAT 
AAACAGACGC CTAAGAGAAA AATTGTCAAG CAAGGACAAA CAACTTTGCC TAAGATTGGT 
GCAAAAAAGG TCACAATGCC TAAAAACCCA AATCAACCCA AAAGAGGTGA AAATTTGAAT 
AATAAAGATG CAAAACCGAA AACACTTCCT GAACAGGTTA TACTGAAGAC TCTGCCTTCT 
TCTCAGAGAC CTTTAAAAAG TGAACCATCT GTTGTCCAAA AAAGTGTGTC TCTTGATGTG 
TGTGACAATA ATAACAAAGG CAGTGTTTCT GAACAGAAGC CTCATGAACC TCTAACTAAT 
CTCACAGCCA AAACCAGTGA TGCAGAAGCA TTTCAGTCAC CATGCATACT CGACTCACAG 
AAGACACTGA ACAATCAAGA AAAACAGAAG TTGGTGTTAG AATGCCAAAA TATTTCAAAT 
CTGGATAAAT TAATAAAACG TGAACTGGAA TCAAAACAGA TCTGTTCAGT TAAAAGTGAA 
ACAAATGTTT CTGGCCACAA AAAAATAGAT CACTGCACCA CAGCTAAAAT ACCTTGTCAT 
TCTGATGAGA CTGATAATGT CGATCCAAAA TTTTATAGTA CCACTGCTCT AAAATCCATG 
ATTTCAAATC CTAAAGAAAA CTCTTTGAAC TCTAATCCAG TTTGTGACTT AGACTCAGCA 
CACGTAGAAC AACTCCATTC AGAATTAGAT AGGAAGAAGC AAGTAGGGAG AAAAGATACA 
AACCAAAAAT TAAGCATTAA ATGTGTGAAA GATGTTTTAC CTTGGGTTCC TGAAAAGACA 
AATGGTACCT TAAATTCTGG TCAAGATGAC AGAAAATCTA AAATTCATGT AGAAGAACAG 
ACAGTTCCTA GTCAGTTGTC TGATGACTCT GCCATGAATG AAGACAATCA TGCTACAGTA 
GAGTCACATA TTTCCTCCAA GTGTTTTTTG GAACAAATAT CAGGGAAAAA TTCTAAAGAT 
ACGGAAACAA CAGAAACTTC AGAGAGCCAC GAAACTCCAG AAGCACCATT CATGAGTCAC 
TGGAATTTGA GTACCAATGT TCTGCATCAG AGAGAGAGTC CTGAATCAGA CAGTGGCAGT 
GTCACAACAT CCTCTGATGA CATAAAGCCC AGATCTGAAG ACTATGATGC TGGAGGGTCT 
CAGGATGATG ATGGGTCAAA TGACAGAGGG GTTTCTAAAT GTGGCACTAT GCTGTGCCAT 
GATTTTCTTG GAAGGAGCAG CAGTGATACC AGTACTCCTG AAGAATTAAA AATATATGAT 
AGCAACTTAA GAATTGAAGT GAAAATGAAA AAGCAAGGTA GTAGTGATCT TTTCCAAGTT 
AATTCAACAA GTGATGATGA GATTCCCAGG AAAAGGCCAG AAATTTGGTC TCGATCTACA 
ATAGTTCATC CTAGGGAAAA AGAAAATATT CCACGAGGCA GTGTCCAGTT TGTACAGGAG 
GTAGATCAGG TTTCTTCCTC AGCAGATGAA ACAGAAGATG AAAGGTCTGA AGCTGAAAAT 
GTTGCAGAAA ATTTCTCTAC ATCTAACCCA GCTCTTCAGC AGTTTCAGGG GATAATTAAT 
CTAGCTTTTG AAGATGCAAC TGAAAATGAA AGTCATGAGT TTCGTGTAAC TAAAAGTTTT 
AAAAGGTCTG TTTTACTTTC CGTAGATGAA TGTGAAGAGA TAGGATCTGA TGAAGGGGAG 
GTCCATGCTC TCTTTCAACC TTCTGTAGAT TCTCTCTCAC CTTCTGATGT TTTTGATGGC 
ATTTCTCATG AACATCATGG AAGGACCTGC TACTCCAGAT ACTCACATGG AAGTGAAGGT 
GGTGTTTTAG AATGCAGACA AAAGAAAAGC AATACTATAT ATAAAAACAA AAGCTTTCCC 
TTGGGTCTTA GTAGCGTTGA CTCATCAAGA AAAGATAAAC AGAGTGCTTC AGCCACAGAA 
AAAAAGGGTA CAACAGATGT CCTGTCCAAA GGAGGCATAC AGCTTCCTCC AGGAGATAAA 
GTATGTAGTG GAAGCAATGT GGATAATGAC TTTGAGACAC ACAGCAAATT TTCAGATAGT 
GATATAAAAT CTCAAGAAAG ACCATGTCAT TTGGAACTTC GTCAAAGAGA ATCCAATTCC 
GACATACCAA AGAACAGCTT CACAAAGTCT CTGGACTCCT GTCGGAGTCA AGTTCTGCCT 
CAGGAAGGTC AAGTGAAAGA AAGCCATTCT ACAGCTACCG AAAAAGCTAA TATTGCTTTA 
TCTGCAGGAG ACATAGATGG TTGTGACACA GTGGCACAAA TCTACATGTA TGACCATCGG 
CCTTCAAAAA CCCTGTCTCC AATATATGAG ATGGATGTAA CAGAAGCATT TGAGCAGAAA 
ATGGAATCAG AAACACAAGG TACAGACATG GATTTTGAAG ATGAGCAACA CTTTGCAAAA 
CAAGACTGGA CACTATTAAA GCAACTGCTC TCTGAACAGG ACTCTAACTT AAATATCACA 
AATTCTGTTC CTGAAGACTT AAATTTAGCA CAGTATCTAA TTAATCAGAC ACTACTTTTA 
GCACGAGACA GCTCAAAACC TCAGGGTAAA GCACATGTAG ACACTTTGAG CAGATGGAGT 
GAACTAACAT CTCCATTTGA TGATTCCTCA GCAAGCATTA CCATGGCTAG TTTTTCCTCT 
GAAGAATGTT CACCCCAAGG GGAATGGACA ATTCTGGAAC TGGAAACTCA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801606.1
CDS	249..5645
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRLLREEVHTDVTFSIGCTLFKAHKAVLLARVPDFYFHTVGHTDNLKNHEHVTVENFEASEFRTFLQIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803304.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803304.2

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GTCACAAGCA ATGAGTAGCA CATCTGATCT GTTGGACAAA 
ATTTTTAAAG CTGTTGAAGA AAACATTACC ACTGAGAATA GTTGCTCGCT TCTTATGGCT 
CTGGACACAT TACTGAACTC TGACAGTACA AAAGAAATGG GTTTTACGTG CAAGATCCAG 
GCTCTGCGTG ATAAGCTGTG GATCTTCCTG GTTCAGTCTT TCTATGCTGT TCGTCACACA 
GAAAGCTGGA AGCTGATGAG CACAGATGAT CAACAGAAAA TCCAAGCAGC TGCATTTGAC 
AAAGGTGATG ACCGAAGACT TGGCAAAAAG CCTATATTCA GTAGCTCTCA GCAAAGAAAA 
CAGGTTTCTG ACTGTGGTGC TATAAAAAAC AAATCCGCTG GAGGAAGTAA CAAGAAACAG 
TGTCTGAGTT ATCTCTCTGC TCATCAGAAG ATGAAATCTG ATGGATTAGG AGCCTCTGGA 
CATTCATCAA GTACTAATAG AAATTCTATA AATAAAACTT TGAAGCATGA TGATTTAAAG 
GAAAAGGATC CAAAACCAGC ATCCAAGAGT ACAAAAGAAT TAAAAACTGT GGAAAAAAAT 
GTTTCTGGAA AGCCCAAAGC TATCATAAAG CCCAAAACAG AAAATAGTGA TAATGCAAAG 
TCAACAAACC TGTCACCTAG ACAAGCTGTG GAAAGATCAG CAACAGCAGC AGCAAATGAA 
CAGAAAAATT CAGGAAATGG AAAAGGAGTG AGAAATCAGG AAGAGCAAAT TACAGGTGCC 
AGACCCAAGG TACTCACTGG AAATTTAAAT GTGCAAGCCA AAGCAAAGCC TTTGAAGAAA 
GTGACAGGCA AAGATTCTCC ACACCTCCGA ATGGCAGGGC CCTCCAGCAG ATCTACAAAT 
TCAAGTATGG AATTACTGAC TTCCACTGAC TCTCTAGATG AACCAAAAGA AAATGGGTCA 
ATGAAAGAGA AGCTTCCTGG TCATAAACCT TCCTCTTGTG ACTCTCGAGG ACAGACTGTG 
AAAAACAGTG TAGAAGGTAT CAAAACTTCC ACTGTAGCAA TGAAATATCG ACCTGTTTCA 
AAAGATACCA ATGGAATTTC CAATAAAAAA AGCACTCATG AACAGGAATC TAATACACAT 
AACAGTGTGC TAAAGAAGAT CACCAGCAAA GGATACAGTG ATCCAGTACC ACAGGCAATT 
TTAAAAAAAA GGGGAAATGG CAATGGATGT GCTACAGCTC CGCAGAGGAC AAAGAATGCC 
ACAGCTAATC TTGCTAAAAC TCAAGGATCC CAAGGATCAC CAAATTCAGT AAAATCTTCA 
GTCTCTTCAA GGCAATCTGA TGAAAATGTG ACAAAATTGG ACCACAGTGT AACTACAGAT 
AAACAGACGC CTAAGAGAAA AATTGTCAAG CAAGGACAAA CAACTTTGCC TAAGATTGGT 
GCAAAAAAGG TCACAATGCC TAAAAACCCA AATCAACCCA AAAGAGGTGA AAATTTGAAT 
AATAAAGATG CAAAACCGAA AACACTTCCT GAACAGGTTA TACTGAAGAC TCTGCCTTCT 
TCTCAGAGAC CTTTAAAAAG TGAACCATCT GTTGTCCAAA AAAGTGTGTC TCTTGATGTG 
TGTGACAATA ATAACAAAGG CAGTGTTTCT GAACAGAAGC CTCATGAACC TCTAACTAAT 
CTCACAGCCA AAACCAGTGA TGCAGAAGCA TTTCAGTCAC CATGCATACT CGACTCACAG 
AAGACACTGA ACAATCAAGA AAAACAGAAG TTGGTGTTAG AATGCCAAAA TATTTCAAAT 
CTGGATAAAT TAATAAAACG TGAACTGGAA TCAAAACAGA TCTGTTCAGT TAAAAGTGAA 
ACAAATGTTT CTGGCCACAA AAAAATAGAT CACTGCACCA CAGCTAAAAT ACCTTGTCAT 
TCTGATGAGA CTGATAATGT CGATCCAAAA TTTTATAGTA CCACTGCTCT AAAATCCATG 
ATTTCAAATC CTAAAGAAAA CTCTTTGAAC TCTAATCCAG TTTGTGACTT AGACTCAGCA 
CACGTAGAAC AACTCCATTC AGAATTAGAT AGGAAGAAGC AAGTAGGGAG AAAAGATACA 
AACCAAAAAT TAAGCATTAA ATGTGTGAAA GATGTTTTAC CTTGGGTTCC TGAAAAGACA 
AATGGTACCT TAAATTCTGG TCAAGATGAC AGAAAATCTA AAATTCATGT AGAAGAACAG 
ACAGTTCCTA GTCAGTTGTC TGATGACTCT GCCATGAATG AAGACAATCA TGCTACAGTA 
GAGTCACATA TTTCCTCCAA GTGTTTTTTG GAACAAATAT CAGGGAAAAA TTCTAAAGAT 
ACGGAAACAA CAGAAACTTC AGAGAGCCAC GAAACTCCAG AAGCACCATT CATGAGTCAC 
TGGAATTTGA GTACCAATGT TCTGCATCAG AGAGAGAGTC CTGAATCAGA CAGTGGCAGT 
GTCACAACAT CCTCTGATGA CATAAAGCCC AGATCTGAAG ACTATGATGC TGGAGGGTCT 
CAGGATGATG ATGGGTCAAA TGACAGAGGG GTTTCTAAAT GTGGCACTAT GCTGTGCCAT 
GATTTTCTTG GAAGGAGCAG CAGTGATACC AGTACTCCTG AAGAATTAAA AATATATGAT 
AGCAACTTAA GAATTGAAGT GAAAATGAAA AAGCAAGGTA GTAGTGATCT TTTCCAAGTT 
AATTCAACAA GTGATGATGA GATTCCCAGG AAAAGGCCAG AAATTTGGTC TCGATCTACA 
ATAGTTCATC CTAGGGAAAA AGAAAATATT CCACGAGGCA GTGTCCAGTT TGTACAGGAG 
GTAGATCAGG TTTCTTCCTC AGCAGATGAA ACAGAAGATG AAAGGTCTGA AGCTGAAAAT 
GTTGCAGAAA ATTTCTCTAC ATCTAACCCA GCTCTTCAGC AGTTTCAGGG GATAATTAAT 
CTAGCTTTTG AAGATGCAAC TGAAAATGAA AGTCATGAGT TTCGTGTAAC TAAAAGTTTT 
AAAAGGTCTG TTTTACTTTC CGTAGATGAA TGTGAAGAGA TAGGATCTGA TGAAGGGGAG 
GTCCATGCTC TCTTTCAACC TTCTGTAGAT TCTCTCTCAC CTTCTGATGT TTTTGATGGC 
ATTTCTCATG AACATCATGG AAGGACCTGC TACTCCAGAT ACTCACATGG AAGTGAAGGT 
GGTGTTTTAG AATGCAGACA AAAGAAAAGC AATACTATAT ATAAAAACAA AAGCTTTCCC 
TTGGGTCTTA GTAGCGTTGA CTCATCAAGA AAAGATAAAC AGAGTGCTTC AGCCACAGAA 
AAAAAGGGTA CAACAGATGT CCTGTCCAAA GGAGGCATAC AGCTTCCTCC AGGAGATAAA 
GTATGTAGTG GAAGCAATGT GGATAATGAC TTTGAGACAC ACAGCAAATT TTCAGATAGT 
GATATAAAAT CTCAAGAAAG ACCATGTCAT TTGGAACTTC GTCAAAGAGA ATCCAATTCC 
GACATACCAA AGAACAGCTT CACAAAGTCT CTGGACTCCT GTCGGAGTCA AGTTCTGCCT 
CAGGAAGGTC AAGTGAAAGA AAGCCATTCT ACAGCTACCG AAAAAGCTAA TATTGCTTTA 
TCTGCAGGAG ACATAGATGG TTGTGACACA GTGGCACAAA TCTACATGTA TGACCATCGG 
CCTTCAAAAA CCCTGTCTCC AATATATGAG ATGGATGTAA CAGAAGCATT TGAGCAGAAA 
ATGGAATCAG AAACACAAGG TACAGACATG GATTTTGAAG ATGAGCAACA CTTTGCAAAA 
CAAGACTGGA CACTATTAAA GCAACTGCTC TCTGAACAGG ACTCTAACTT AAATATCACA 
AATTCTGTTC CTGAAGACTT AAATTTAGCA CAGTATCTAA TTAATCAGAC ACTACTTTTA 
GCACGAGACA GCTCAAAACC TCAGGGTAAA GCACATGTAG ACACTTTGAG CAGATGGAGT 
GAACTAACAT CTCCATTTGA TGATTCCTCA GCAAGCATTA CCATGGCTAG TTTTTCCTCT 
GAAGAATGTT CACCCCAAGG GGAATGGACA ATTCTGGAAC TGGAAACTCA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803304.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801606.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50256
Clone ID Related Accession (Same CDS sequence)	XM_010803304.2 , XM_010803304.1
Accession Version	XM_010803304.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GTCACAAGCA ATGAGTAGCA CATCTGATCT GTTGGACAAA 
ATTTTTAAAG CTGTTGAAGA AAACATTACC ACTGAGAATA GTTGCTCGCT TCTTATGGCT 
CTGGACACAT TACTGAACTC TGACAGTACA AAAGAAATGG GTTTTACGTG CAAGATCCAG 
GCTCTGCGTG ATAAGCTGTG GATCTTCCTG GTTCAGTCTT TCTATGCTGT TCGTCACACA 
GAAAGCTGGA AGCTGATGAG CACAGATGAT CAACAGAAAA TCCAAGCAGC TGCATTTGAC 
AAAGGTGATG ACCGAAGACT TGGCAAAAAG CCTATATTCA GTAGCTCTCA GCAAAGAAAA 
CAGGTTTCTG ACTGTGGTGC TATAAAAAAC AAATCCGCTG GAGGAAGTAA CAAGAAACAG 
TGTCTGAGTT ATCTCTCTGC TCATCAGAAG ATGAAATCTG ATGGATTAGG AGCCTCTGGA 
CATTCATCAA GTACTAATAG AAATTCTATA AATAAAACTT TGAAGCATGA TGATTTAAAG 
GAAAAGGATC CAAAACCAGC ATCCAAGAGT ACAAAAGAAT TAAAAACTGT GGAAAAAAAT 
GTTTCTGGAA AGCCCAAAGC TATCATAAAG CCCAAAACAG AAAATAGTGA TAATGCAAAG 
TCAACAAACC TGTCACCTAG ACAAGCTGTG GAAAGATCAG CAACAGCAGC AGCAAATGAA 
CAGAAAAATT CAGGAAATGG AAAAGGAGTG AGAAATCAGG AAGAGCAAAT TACAGGTGCC 
AGACCCAAGG TACTCACTGG AAATTTAAAT GTGCAAGCCA AAGCAAAGCC TTTGAAGAAA 
GTGACAGGCA AAGATTCTCC ACACCTCCGA ATGGCAGGGC CCTCCAGCAG ATCTACAAAT 
TCAAGTATGG AATTACTGAC TTCCACTGAC TCTCTAGATG AACCAAAAGA AAATGGGTCA 
ATGAAAGAGA AGCTTCCTGG TCATAAACCT TCCTCTTGTG ACTCTCGAGG ACAGACTGTG 
AAAAACAGTG TAGAAGGTAT CAAAACTTCC ACTGTAGCAA TGAAATATCG ACCTGTTTCA 
AAAGATACCA ATGGAATTTC CAATAAAAAA AGCACTCATG AACAGGAATC TAATACACAT 
AACAGTGTGC TAAAGAAGAT CACCAGCAAA GGATACAGTG ATCCAGTACC ACAGGCAATT 
TTAAAAAAAA GGGGAAATGG CAATGGATGT GCTACAGCTC CGCAGAGGAC AAAGAATGCC 
ACAGCTAATC TTGCTAAAAC TCAAGGATCC CAAGGATCAC CAAATTCAGT AAAATCTTCA 
GTCTCTTCAA GGCAATCTGA TGAAAATGTG ACAAAATTGG ACCACAGTGT AACTACAGAT 
AAACAGACGC CTAAGAGAAA AATTGTCAAG CAAGGACAAA CAACTTTGCC TAAGATTGGT 
GCAAAAAAGG TCACAATGCC TAAAAACCCA AATCAACCCA AAAGAGGTGA AAATTTGAAT 
AATAAAGATG CAAAACCGAA AACACTTCCT GAACAGGTTA TACTGAAGAC TCTGCCTTCT 
TCTCAGAGAC CTTTAAAAAG TGAACCATCT GTTGTCCAAA AAAGTGTGTC TCTTGATGTG 
TGTGACAATA ATAACAAAGG CAGTGTTTCT GAACAGAAGC CTCATGAACC TCTAACTAAT 
CTCACAGCCA AAACCAGTGA TGCAGAAGCA TTTCAGTCAC CATGCATACT CGACTCACAG 
AAGACACTGA ACAATCAAGA AAAACAGAAG TTGGTGTTAG AATGCCAAAA TATTTCAAAT 
CTGGATAAAT TAATAAAACG TGAACTGGAA TCAAAACAGA TCTGTTCAGT TAAAAGTGAA 
ACAAATGTTT CTGGCCACAA AAAAATAGAT CACTGCACCA CAGCTAAAAT ACCTTGTCAT 
TCTGATGAGA CTGATAATGT CGATCCAAAA TTTTATAGTA CCACTGCTCT AAAATCCATG 
ATTTCAAATC CTAAAGAAAA CTCTTTGAAC TCTAATCCAG TTTGTGACTT AGACTCAGCA 
CACGTAGAAC AACTCCATTC AGAATTAGAT AGGAAGAAGC AAGTAGGGAG AAAAGATACA 
AACCAAAAAT TAAGCATTAA ATGTGTGAAA GATGTTTTAC CTTGGGTTCC TGAAAAGACA 
AATGGTACCT TAAATTCTGG TCAAGATGAC AGAAAATCTA AAATTCATGT AGAAGAACAG 
ACAGTTCCTA GTCAGTTGTC TGATGACTCT GCCATGAATG AAGACAATCA TGCTACAGTA 
GAGTCACATA TTTCCTCCAA GTGTTTTTTG GAACAAATAT CAGGGAAAAA TTCTAAAGAT 
ACGGAAACAA CAGAAACTTC AGAGAGCCAC GAAACTCCAG AAGCACCATT CATGAGTCAC 
TGGAATTTGA GTACCAATGT TCTGCATCAG AGAGAGAGTC CTGAATCAGA CAGTGGCAGT 
GTCACAACAT CCTCTGATGA CATAAAGCCC AGATCTGAAG ACTATGATGC TGGAGGGTCT 
CAGGATGATG ATGGGTCAAA TGACAGAGGG GTTTCTAAAT GTGGCACTAT GCTGTGCCAT 
GATTTTCTTG GAAGGAGCAG CAGTGATACC AGTACTCCTG AAGAATTAAA AATATATGAT 
AGCAACTTAA GAATTGAAGT GAAAATGAAA AAGCAAGGTA GTAGTGATCT TTTCCAAGTT 
AATTCAACAA GTGATGATGA GATTCCCAGG AAAAGGCCAG AAATTTGGTC TCGATCTACA 
ATAGTTCATC CTAGGGAAAA AGAAAATATT CCACGAGGCA GTGTCCAGTT TGTACAGGAG 
GTAGATCAGG TTTCTTCCTC AGCAGATGAA ACAGAAGATG AAAGGTCTGA AGCTGAAAAT 
GTTGCAGAAA ATTTCTCTAC ATCTAACCCA GCTCTTCAGC AGTTTCAGGG GATAATTAAT 
CTAGCTTTTG AAGATGCAAC TGAAAATGAA AGTCATGAGT TTCGTGTAAC TAAAAGTTTT 
AAAAGGTCTG TTTTACTTTC CGTAGATGAA TGTGAAGAGA TAGGATCTGA TGAAGGGGAG 
GTCCATGCTC TCTTTCAACC TTCTGTAGAT TCTCTCTCAC CTTCTGATGT TTTTGATGGC 
ATTTCTCATG AACATCATGG AAGGACCTGC TACTCCAGAT ACTCACATGG AAGTGAAGGT 
GGTGTTTTAG AATGCAGACA AAAGAAAAGC AATACTATAT ATAAAAACAA AAGCTTTCCC 
TTGGGTCTTA GTAGCGTTGA CTCATCAAGA AAAGATAAAC AGAGTGCTTC AGCCACAGAA 
AAAAAGGGTA CAACAGATGT CCTGTCCAAA GGAGGCATAC AGCTTCCTCC AGGAGATAAA 
GTATGTAGTG GAAGCAATGT GGATAATGAC TTTGAGACAC ACAGCAAATT TTCAGATAGT 
GATATAAAAT CTCAAGAAAG ACCATGTCAT TTGGAACTTC GTCAAAGAGA ATCCAATTCC 
GACATACCAA AGAACAGCTT CACAAAGTCT CTGGACTCCT GTCGGAGTCA AGTTCTGCCT 
CAGGAAGGTC AAGTGAAAGA AAGCCATTCT ACAGCTACCG AAAAAGCTAA TATTGCTTTA 
TCTGCAGGAG ACATAGATGG TTGTGACACA GTGGCACAAA TCTACATGTA TGACCATCGG 
CCTTCAAAAA CCCTGTCTCC AATATATGAG ATGGATGTAA CAGAAGCATT TGAGCAGAAA 
ATGGAATCAG AAACACAAGG TACAGACATG GATTTTGAAG ATGAGCAACA CTTTGCAAAA 
CAAGACTGGA CACTATTAAA GCAACTGCTC TCTGAACAGG ACTCTAACTT AAATATCACA 
AATTCTGTTC CTGAAGACTT AAATTTAGCA CAGTATCTAA TTAATCAGAC ACTACTTTTA 
GCACGAGACA GCTCAAAACC TCAGGGTAAA GCACATGTAG ACACTTTGAG CAGATGGAGT 
GAACTAACAT CTCCATTTGA TGATTCCTCA GCAAGCATTA CCATGGCTAG TTTTTCCTCT 
GAAGAATGTT CACCCCAAGG GGAATGGACA ATTCTGGAAC TGGAAACTCA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801606.1
CDS	225..5621
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRLLREEVHTDVTFSIGCTLFKAHKAVLLARVPDFYFHTVGHTDNLKNHEHVTVENFEASEFRTFLQIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803304.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803304.1

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GTCACAAGCA ATGAGTAGCA CATCTGATCT GTTGGACAAA 
ATTTTTAAAG CTGTTGAAGA AAACATTACC ACTGAGAATA GTTGCTCGCT TCTTATGGCT 
CTGGACACAT TACTGAACTC TGACAGTACA AAAGAAATGG GTTTTACGTG CAAGATCCAG 
GCTCTGCGTG ATAAGCTGTG GATCTTCCTG GTTCAGTCTT TCTATGCTGT TCGTCACACA 
GAAAGCTGGA AGCTGATGAG CACAGATGAT CAACAGAAAA TCCAAGCAGC TGCATTTGAC 
AAAGGTGATG ACCGAAGACT TGGCAAAAAG CCTATATTCA GTAGCTCTCA GCAAAGAAAA 
CAGGTTTCTG ACTGTGGTGC TATAAAAAAC AAATCCGCTG GAGGAAGTAA CAAGAAACAG 
TGTCTGAGTT ATCTCTCTGC TCATCAGAAG ATGAAATCTG ATGGATTAGG AGCCTCTGGA 
CATTCATCAA GTACTAATAG AAATTCTATA AATAAAACTT TGAAGCATGA TGATTTAAAG 
GAAAAGGATC CAAAACCAGC ATCCAAGAGT ACAAAAGAAT TAAAAACTGT GGAAAAAAAT 
GTTTCTGGAA AGCCCAAAGC TATCATAAAG CCCAAAACAG AAAATAGTGA TAATGCAAAG 
TCAACAAACC TGTCACCTAG ACAAGCTGTG GAAAGATCAG CAACAGCAGC AGCAAATGAA 
CAGAAAAATT CAGGAAATGG AAAAGGAGTG AGAAATCAGG AAGAGCAAAT TACAGGTGCC 
AGACCCAAGG TACTCACTGG AAATTTAAAT GTGCAAGCCA AAGCAAAGCC TTTGAAGAAA 
GTGACAGGCA AAGATTCTCC ACACCTCCGA ATGGCAGGGC CCTCCAGCAG ATCTACAAAT 
TCAAGTATGG AATTACTGAC TTCCACTGAC TCTCTAGATG AACCAAAAGA AAATGGGTCA 
ATGAAAGAGA AGCTTCCTGG TCATAAACCT TCCTCTTGTG ACTCTCGAGG ACAGACTGTG 
AAAAACAGTG TAGAAGGTAT CAAAACTTCC ACTGTAGCAA TGAAATATCG ACCTGTTTCA 
AAAGATACCA ATGGAATTTC CAATAAAAAA AGCACTCATG AACAGGAATC TAATACACAT 
AACAGTGTGC TAAAGAAGAT CACCAGCAAA GGATACAGTG ATCCAGTACC ACAGGCAATT 
TTAAAAAAAA GGGGAAATGG CAATGGATGT GCTACAGCTC CGCAGAGGAC AAAGAATGCC 
ACAGCTAATC TTGCTAAAAC TCAAGGATCC CAAGGATCAC CAAATTCAGT AAAATCTTCA 
GTCTCTTCAA GGCAATCTGA TGAAAATGTG ACAAAATTGG ACCACAGTGT AACTACAGAT 
AAACAGACGC CTAAGAGAAA AATTGTCAAG CAAGGACAAA CAACTTTGCC TAAGATTGGT 
GCAAAAAAGG TCACAATGCC TAAAAACCCA AATCAACCCA AAAGAGGTGA AAATTTGAAT 
AATAAAGATG CAAAACCGAA AACACTTCCT GAACAGGTTA TACTGAAGAC TCTGCCTTCT 
TCTCAGAGAC CTTTAAAAAG TGAACCATCT GTTGTCCAAA AAAGTGTGTC TCTTGATGTG 
TGTGACAATA ATAACAAAGG CAGTGTTTCT GAACAGAAGC CTCATGAACC TCTAACTAAT 
CTCACAGCCA AAACCAGTGA TGCAGAAGCA TTTCAGTCAC CATGCATACT CGACTCACAG 
AAGACACTGA ACAATCAAGA AAAACAGAAG TTGGTGTTAG AATGCCAAAA TATTTCAAAT 
CTGGATAAAT TAATAAAACG TGAACTGGAA TCAAAACAGA TCTGTTCAGT TAAAAGTGAA 
ACAAATGTTT CTGGCCACAA AAAAATAGAT CACTGCACCA CAGCTAAAAT ACCTTGTCAT 
TCTGATGAGA CTGATAATGT CGATCCAAAA TTTTATAGTA CCACTGCTCT AAAATCCATG 
ATTTCAAATC CTAAAGAAAA CTCTTTGAAC TCTAATCCAG TTTGTGACTT AGACTCAGCA 
CACGTAGAAC AACTCCATTC AGAATTAGAT AGGAAGAAGC AAGTAGGGAG AAAAGATACA 
AACCAAAAAT TAAGCATTAA ATGTGTGAAA GATGTTTTAC CTTGGGTTCC TGAAAAGACA 
AATGGTACCT TAAATTCTGG TCAAGATGAC AGAAAATCTA AAATTCATGT AGAAGAACAG 
ACAGTTCCTA GTCAGTTGTC TGATGACTCT GCCATGAATG AAGACAATCA TGCTACAGTA 
GAGTCACATA TTTCCTCCAA GTGTTTTTTG GAACAAATAT CAGGGAAAAA TTCTAAAGAT 
ACGGAAACAA CAGAAACTTC AGAGAGCCAC GAAACTCCAG AAGCACCATT CATGAGTCAC 
TGGAATTTGA GTACCAATGT TCTGCATCAG AGAGAGAGTC CTGAATCAGA CAGTGGCAGT 
GTCACAACAT CCTCTGATGA CATAAAGCCC AGATCTGAAG ACTATGATGC TGGAGGGTCT 
CAGGATGATG ATGGGTCAAA TGACAGAGGG GTTTCTAAAT GTGGCACTAT GCTGTGCCAT 
GATTTTCTTG GAAGGAGCAG CAGTGATACC AGTACTCCTG AAGAATTAAA AATATATGAT 
AGCAACTTAA GAATTGAAGT GAAAATGAAA AAGCAAGGTA GTAGTGATCT TTTCCAAGTT 
AATTCAACAA GTGATGATGA GATTCCCAGG AAAAGGCCAG AAATTTGGTC TCGATCTACA 
ATAGTTCATC CTAGGGAAAA AGAAAATATT CCACGAGGCA GTGTCCAGTT TGTACAGGAG 
GTAGATCAGG TTTCTTCCTC AGCAGATGAA ACAGAAGATG AAAGGTCTGA AGCTGAAAAT 
GTTGCAGAAA ATTTCTCTAC ATCTAACCCA GCTCTTCAGC AGTTTCAGGG GATAATTAAT 
CTAGCTTTTG AAGATGCAAC TGAAAATGAA AGTCATGAGT TTCGTGTAAC TAAAAGTTTT 
AAAAGGTCTG TTTTACTTTC CGTAGATGAA TGTGAAGAGA TAGGATCTGA TGAAGGGGAG 
GTCCATGCTC TCTTTCAACC TTCTGTAGAT TCTCTCTCAC CTTCTGATGT TTTTGATGGC 
ATTTCTCATG AACATCATGG AAGGACCTGC TACTCCAGAT ACTCACATGG AAGTGAAGGT 
GGTGTTTTAG AATGCAGACA AAAGAAAAGC AATACTATAT ATAAAAACAA AAGCTTTCCC 
TTGGGTCTTA GTAGCGTTGA CTCATCAAGA AAAGATAAAC AGAGTGCTTC AGCCACAGAA 
AAAAAGGGTA CAACAGATGT CCTGTCCAAA GGAGGCATAC AGCTTCCTCC AGGAGATAAA 
GTATGTAGTG GAAGCAATGT GGATAATGAC TTTGAGACAC ACAGCAAATT TTCAGATAGT 
GATATAAAAT CTCAAGAAAG ACCATGTCAT TTGGAACTTC GTCAAAGAGA ATCCAATTCC 
GACATACCAA AGAACAGCTT CACAAAGTCT CTGGACTCCT GTCGGAGTCA AGTTCTGCCT 
CAGGAAGGTC AAGTGAAAGA AAGCCATTCT ACAGCTACCG AAAAAGCTAA TATTGCTTTA 
TCTGCAGGAG ACATAGATGG TTGTGACACA GTGGCACAAA TCTACATGTA TGACCATCGG 
CCTTCAAAAA CCCTGTCTCC AATATATGAG ATGGATGTAA CAGAAGCATT TGAGCAGAAA 
ATGGAATCAG AAACACAAGG TACAGACATG GATTTTGAAG ATGAGCAACA CTTTGCAAAA 
CAAGACTGGA CACTATTAAA GCAACTGCTC TCTGAACAGG ACTCTAACTT AAATATCACA 
AATTCTGTTC CTGAAGACTT AAATTTAGCA CAGTATCTAA TTAATCAGAC ACTACTTTTA 
GCACGAGACA GCTCAAAACC TCAGGGTAAA GCACATGTAG ACACTTTGAG CAGATGGAGT 
GAACTAACAT CTCCATTTGA TGATTCCTCA GCAAGCATTA CCATGGCTAG TTTTTCCTCT 
GAAGAATGTT CACCCCAAGG GGAATGGACA ATTCTGGAAC TGGAAACTCA GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803305.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801607.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.

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CloneID	OBa50257
Clone ID Related Accession (Same CDS sequence)	XM_010803305.1 , XM_010803305.2
Accession Version	XM_010803305.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GGGTTTTACG TGCAAGATCC AGGCTCTGCG TGATAAGCTG 
TGGATCTTCC TGGTTCAGTC TTTCTATGCT GTTCGTCACA CAGAAAGCTG GAAGCTGATG 
AGCACAGATG ATCAACAGAA AATCCAAGCA GCTGCATTTG ACAAAGGTGA TGACCGAAGA 
CTTGGCAAAA AGCCTATATT CAGTAGCTCT CAGCAAAGAA AACAGGTTTC TGACTGTGGT 
GCTATAAAAA ACAAATCCGC TGGAGGAAGT AACAAGAAAC AGTGTCTGAG TTATCTCTCT 
GCTCATCAGA AGATGAAATC TGATGGATTA GGAGCCTCTG GACATTCATC AAGTACTAAT 
AGAAATTCTA TAAATAAAAC TTTGAAGCAT GATGATTTAA AGGAAAAGGA TCCAAAACCA 
GCATCCAAGA GTACAAAAGA ATTAAAAACT GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA 
GCTATCATAA AGCCCAAAAC AGAAAATAGT GATAATGCAA AGTCAACAAA CCTGTCACCT 
AGACAAGCTG TGGAAAGATC AGCAACAGCA GCAGCAAATG AACAGAAAAA TTCAGGAAAT 
GGAAAAGGAG TGAGAAATCA GGAAGAGCAA ATTACAGGTG CCAGACCCAA GGTACTCACT 
GGAAATTTAA ATGTGCAAGC CAAAGCAAAG CCTTTGAAGA AAGTGACAGG CAAAGATTCT 
CCACACCTCC GAATGGCAGG GCCCTCCAGC AGATCTACAA ATTCAAGTAT GGAATTACTG 
ACTTCCACTG ACTCTCTAGA TGAACCAAAA GAAAATGGGT CAATGAAAGA GAAGCTTCCT 
GGTCATAAAC CTTCCTCTTG TGACTCTCGA GGACAGACTG TGAAAAACAG TGTAGAAGGT 
ATCAAAACTT CCACTGTAGC AATGAAATAT CGACCTGTTT CAAAAGATAC CAATGGAATT 
TCCAATAAAA AAAGCACTCA TGAACAGGAA TCTAATACAC ATAACAGTGT GCTAAAGAAG 
ATCACCAGCA AAGGATACAG TGATCCAGTA CCACAGGCAA TTTTAAAAAA AAGGGGAAAT 
GGCAATGGAT GTGCTACAGC TCCGCAGAGG ACAAAGAATG CCACAGCTAA TCTTGCTAAA 
ACTCAAGGAT CCCAAGGATC ACCAAATTCA GTAAAATCTT CAGTCTCTTC AAGGCAATCT 
GATGAAAATG TGACAAAATT GGACCACAGT GTAACTACAG ATAAACAGAC GCCTAAGAGA 
AAAATTGTCA AGCAAGGACA AACAACTTTG CCTAAGATTG GTGCAAAAAA GGTCACAATG 
CCTAAAAACC CAAATCAACC CAAAAGAGGT GAAAATTTGA ATAATAAAGA TGCAAAACCG 
AAAACACTTC CTGAACAGGT TATACTGAAG ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA 
AGTGAACCAT CTGTTGTCCA AAAAAGTGTG TCTCTTGATG TGTGTGACAA TAATAACAAA 
GGCAGTGTTT CTGAACAGAA GCCTCATGAA CCTCTAACTA ATCTCACAGC CAAAACCAGT 
GATGCAGAAG CATTTCAGTC ACCATGCATA CTCGACTCAC AGAAGACACT GAACAATCAA 
GAAAAACAGA AGTTGGTGTT AGAATGCCAA AATATTTCAA ATCTGGATAA ATTAATAAAA 
CGTGAACTGG AATCAAAACA GATCTGTTCA GTTAAAAGTG AAACAAATGT TTCTGGCCAC 
AAAAAAATAG ATCACTGCAC CACAGCTAAA ATACCTTGTC ATTCTGATGA GACTGATAAT 
GTCGATCCAA AATTTTATAG TACCACTGCT CTAAAATCCA TGATTTCAAA TCCTAAAGAA 
AACTCTTTGA ACTCTAATCC AGTTTGTGAC TTAGACTCAG CACACGTAGA ACAACTCCAT 
TCAGAATTAG ATAGGAAGAA GCAAGTAGGG AGAAAAGATA CAAACCAAAA ATTAAGCATT 
AAATGTGTGA AAGATGTTTT ACCTTGGGTT CCTGAAAAGA CAAATGGTAC CTTAAATTCT 
GGTCAAGATG ACAGAAAATC TAAAATTCAT GTAGAAGAAC AGACAGTTCC TAGTCAGTTG 
TCTGATGACT CTGCCATGAA TGAAGACAAT CATGCTACAG TAGAGTCACA TATTTCCTCC 
AAGTGTTTTT TGGAACAAAT ATCAGGGAAA AATTCTAAAG ATACGGAAAC AACAGAAACT 
TCAGAGAGCC ACGAAACTCC AGAAGCACCA TTCATGAGTC ACTGGAATTT GAGTACCAAT 
GTTCTGCATC AGAGAGAGAG TCCTGAATCA GACAGTGGCA GTGTCACAAC ATCCTCTGAT 
GACATAAAGC CCAGATCTGA AGACTATGAT GCTGGAGGGT CTCAGGATGA TGATGGGTCA 
AATGACAGAG GGGTTTCTAA ATGTGGCACT ATGCTGTGCC ATGATTTTCT TGGAAGGAGC 
AGCAGTGATA CCAGTACTCC TGAAGAATTA AAAATATATG ATAGCAACTT AAGAATTGAA 
GTGAAAATGA AAAAGCAAGG TAGTAGTGAT CTTTTCCAAG TTAATTCAAC AAGTGATGAT 
GAGATTCCCA GGAAAAGGCC AGAAATTTGG TCTCGATCTA CAATAGTTCA TCCTAGGGAA 
AAAGAAAATA TTCCACGAGG CAGTGTCCAG TTTGTACAGG AGGTAGATCA GGTTTCTTCC 
TCAGCAGATG AAACAGAAGA TGAAAGGTCT GAAGCTGAAA ATGTTGCAGA AAATTTCTCT 
ACATCTAACC CAGCTCTTCA GCAGTTTCAG GGGATAATTA ATCTAGCTTT TGAAGATGCA 
ACTGAAAATG AAAGTCATGA GTTTCGTGTA ACTAAAAGTT TTAAAAGGTC TGTTTTACTT 
TCCGTAGATG AATGTGAAGA GATAGGATCT GATGAAGGGG AGGTCCATGC TCTCTTTCAA 
CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT GTTTTTGATG GCATTTCTCA TGAACATCAT 
GGAAGGACCT GCTACTCCAG ATACTCACAT GGAAGTGAAG GTGGTGTTTT AGAATGCAGA 
CAAAAGAAAA GCAATACTAT ATATAAAAAC AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT 
GACTCATCAA GAAAAGATAA ACAGAGTGCT TCAGCCACAG AAAAAAAGGG TACAACAGAT 
GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT CCAGGAGATA AAGTATGTAG TGGAAGCAAT 
GTGGATAATG ACTTTGAGAC ACACAGCAAA TTTTCAGATA GTGATATAAA ATCTCAAGAA 
AGACCATGTC ATTTGGAACT TCGTCAAAGA GAATCCAATT CCGACATACC AAAGAACAGC 
TTCACAAAGT CTCTGGACTC CTGTCGGAGT CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA 
GAAAGCCATT CTACAGCTAC CGAAAAAGCT AATATTGCTT TATCTGCAGG AGACATAGAT 
GGTTGTGACA CAGTGGCACA AATCTACATG TATGACCATC GGCCTTCAAA AACCCTGTCT 
CCAATATATG AGATGGATGT AACAGAAGCA TTTGAGCAGA AAATGGAATC AGAAACACAA 
GGTACAGACA TGGATTTTGA AGATGAGCAA CACTTTGCAA AACAAGACTG GACACTATTA 
AAGCAACTGC TCTCTGAACA GGACTCTAAC TTAAATATCA CAAATTCTGT TCCTGAAGAC 
TTAAATTTAG CACAGTATCT AATTAATCAG ACACTACTTT TAGCACGAGA CAGCTCAAAA 
CCTCAGGGTA AAGCACATGT AGACACTTTG AGCAGATGGA GTGAACTAAC ATCTCCATTT 
GATGATTCCT CAGCAAGCAT TACCATGGCT AGTTTTTCCT CTGAAGAATG TTCACCCCAA 
GGGGAATGGA CAATTCTGGA ACTGGAAACT CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801607.1
CDS	225..5483
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRLLREEVHTDVTFSIGCTLFKAHKAVLLARVPDFYFHTVGHTDNLKNHEHVTVENFEASEFRTFLQIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803305.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803305.1

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GGGTTTTACG TGCAAGATCC AGGCTCTGCG TGATAAGCTG 
TGGATCTTCC TGGTTCAGTC TTTCTATGCT GTTCGTCACA CAGAAAGCTG GAAGCTGATG 
AGCACAGATG ATCAACAGAA AATCCAAGCA GCTGCATTTG ACAAAGGTGA TGACCGAAGA 
CTTGGCAAAA AGCCTATATT CAGTAGCTCT CAGCAAAGAA AACAGGTTTC TGACTGTGGT 
GCTATAAAAA ACAAATCCGC TGGAGGAAGT AACAAGAAAC AGTGTCTGAG TTATCTCTCT 
GCTCATCAGA AGATGAAATC TGATGGATTA GGAGCCTCTG GACATTCATC AAGTACTAAT 
AGAAATTCTA TAAATAAAAC TTTGAAGCAT GATGATTTAA AGGAAAAGGA TCCAAAACCA 
GCATCCAAGA GTACAAAAGA ATTAAAAACT GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA 
GCTATCATAA AGCCCAAAAC AGAAAATAGT GATAATGCAA AGTCAACAAA CCTGTCACCT 
AGACAAGCTG TGGAAAGATC AGCAACAGCA GCAGCAAATG AACAGAAAAA TTCAGGAAAT 
GGAAAAGGAG TGAGAAATCA GGAAGAGCAA ATTACAGGTG CCAGACCCAA GGTACTCACT 
GGAAATTTAA ATGTGCAAGC CAAAGCAAAG CCTTTGAAGA AAGTGACAGG CAAAGATTCT 
CCACACCTCC GAATGGCAGG GCCCTCCAGC AGATCTACAA ATTCAAGTAT GGAATTACTG 
ACTTCCACTG ACTCTCTAGA TGAACCAAAA GAAAATGGGT CAATGAAAGA GAAGCTTCCT 
GGTCATAAAC CTTCCTCTTG TGACTCTCGA GGACAGACTG TGAAAAACAG TGTAGAAGGT 
ATCAAAACTT CCACTGTAGC AATGAAATAT CGACCTGTTT CAAAAGATAC CAATGGAATT 
TCCAATAAAA AAAGCACTCA TGAACAGGAA TCTAATACAC ATAACAGTGT GCTAAAGAAG 
ATCACCAGCA AAGGATACAG TGATCCAGTA CCACAGGCAA TTTTAAAAAA AAGGGGAAAT 
GGCAATGGAT GTGCTACAGC TCCGCAGAGG ACAAAGAATG CCACAGCTAA TCTTGCTAAA 
ACTCAAGGAT CCCAAGGATC ACCAAATTCA GTAAAATCTT CAGTCTCTTC AAGGCAATCT 
GATGAAAATG TGACAAAATT GGACCACAGT GTAACTACAG ATAAACAGAC GCCTAAGAGA 
AAAATTGTCA AGCAAGGACA AACAACTTTG CCTAAGATTG GTGCAAAAAA GGTCACAATG 
CCTAAAAACC CAAATCAACC CAAAAGAGGT GAAAATTTGA ATAATAAAGA TGCAAAACCG 
AAAACACTTC CTGAACAGGT TATACTGAAG ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA 
AGTGAACCAT CTGTTGTCCA AAAAAGTGTG TCTCTTGATG TGTGTGACAA TAATAACAAA 
GGCAGTGTTT CTGAACAGAA GCCTCATGAA CCTCTAACTA ATCTCACAGC CAAAACCAGT 
GATGCAGAAG CATTTCAGTC ACCATGCATA CTCGACTCAC AGAAGACACT GAACAATCAA 
GAAAAACAGA AGTTGGTGTT AGAATGCCAA AATATTTCAA ATCTGGATAA ATTAATAAAA 
CGTGAACTGG AATCAAAACA GATCTGTTCA GTTAAAAGTG AAACAAATGT TTCTGGCCAC 
AAAAAAATAG ATCACTGCAC CACAGCTAAA ATACCTTGTC ATTCTGATGA GACTGATAAT 
GTCGATCCAA AATTTTATAG TACCACTGCT CTAAAATCCA TGATTTCAAA TCCTAAAGAA 
AACTCTTTGA ACTCTAATCC AGTTTGTGAC TTAGACTCAG CACACGTAGA ACAACTCCAT 
TCAGAATTAG ATAGGAAGAA GCAAGTAGGG AGAAAAGATA CAAACCAAAA ATTAAGCATT 
AAATGTGTGA AAGATGTTTT ACCTTGGGTT CCTGAAAAGA CAAATGGTAC CTTAAATTCT 
GGTCAAGATG ACAGAAAATC TAAAATTCAT GTAGAAGAAC AGACAGTTCC TAGTCAGTTG 
TCTGATGACT CTGCCATGAA TGAAGACAAT CATGCTACAG TAGAGTCACA TATTTCCTCC 
AAGTGTTTTT TGGAACAAAT ATCAGGGAAA AATTCTAAAG ATACGGAAAC AACAGAAACT 
TCAGAGAGCC ACGAAACTCC AGAAGCACCA TTCATGAGTC ACTGGAATTT GAGTACCAAT 
GTTCTGCATC AGAGAGAGAG TCCTGAATCA GACAGTGGCA GTGTCACAAC ATCCTCTGAT 
GACATAAAGC CCAGATCTGA AGACTATGAT GCTGGAGGGT CTCAGGATGA TGATGGGTCA 
AATGACAGAG GGGTTTCTAA ATGTGGCACT ATGCTGTGCC ATGATTTTCT TGGAAGGAGC 
AGCAGTGATA CCAGTACTCC TGAAGAATTA AAAATATATG ATAGCAACTT AAGAATTGAA 
GTGAAAATGA AAAAGCAAGG TAGTAGTGAT CTTTTCCAAG TTAATTCAAC AAGTGATGAT 
GAGATTCCCA GGAAAAGGCC AGAAATTTGG TCTCGATCTA CAATAGTTCA TCCTAGGGAA 
AAAGAAAATA TTCCACGAGG CAGTGTCCAG TTTGTACAGG AGGTAGATCA GGTTTCTTCC 
TCAGCAGATG AAACAGAAGA TGAAAGGTCT GAAGCTGAAA ATGTTGCAGA AAATTTCTCT 
ACATCTAACC CAGCTCTTCA GCAGTTTCAG GGGATAATTA ATCTAGCTTT TGAAGATGCA 
ACTGAAAATG AAAGTCATGA GTTTCGTGTA ACTAAAAGTT TTAAAAGGTC TGTTTTACTT 
TCCGTAGATG AATGTGAAGA GATAGGATCT GATGAAGGGG AGGTCCATGC TCTCTTTCAA 
CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT GTTTTTGATG GCATTTCTCA TGAACATCAT 
GGAAGGACCT GCTACTCCAG ATACTCACAT GGAAGTGAAG GTGGTGTTTT AGAATGCAGA 
CAAAAGAAAA GCAATACTAT ATATAAAAAC AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT 
GACTCATCAA GAAAAGATAA ACAGAGTGCT TCAGCCACAG AAAAAAAGGG TACAACAGAT 
GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT CCAGGAGATA AAGTATGTAG TGGAAGCAAT 
GTGGATAATG ACTTTGAGAC ACACAGCAAA TTTTCAGATA GTGATATAAA ATCTCAAGAA 
AGACCATGTC ATTTGGAACT TCGTCAAAGA GAATCCAATT CCGACATACC AAAGAACAGC 
TTCACAAAGT CTCTGGACTC CTGTCGGAGT CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA 
GAAAGCCATT CTACAGCTAC CGAAAAAGCT AATATTGCTT TATCTGCAGG AGACATAGAT 
GGTTGTGACA CAGTGGCACA AATCTACATG TATGACCATC GGCCTTCAAA AACCCTGTCT 
CCAATATATG AGATGGATGT AACAGAAGCA TTTGAGCAGA AAATGGAATC AGAAACACAA 
GGTACAGACA TGGATTTTGA AGATGAGCAA CACTTTGCAA AACAAGACTG GACACTATTA 
AAGCAACTGC TCTCTGAACA GGACTCTAAC TTAAATATCA CAAATTCTGT TCCTGAAGAC 
TTAAATTTAG CACAGTATCT AATTAATCAG ACACTACTTT TAGCACGAGA CAGCTCAAAA 
CCTCAGGGTA AAGCACATGT AGACACTTTG AGCAGATGGA GTGAACTAAC ATCTCCATTT 
GATGATTCCT CAGCAAGCAT TACCATGGCT AGTTTTTCCT CTGAAGAATG TTCACCCCAA 
GGGGAATGGA CAATTCTGGA ACTGGAAACT CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803305.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801607.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.

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CloneID	OBa50257
Clone ID Related Accession (Same CDS sequence)	XM_010803305.1 , XM_010803305.2
Accession Version	XM_010803305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GGGTTTTACG TGCAAGATCC AGGCTCTGCG TGATAAGCTG 
TGGATCTTCC TGGTTCAGTC TTTCTATGCT GTTCGTCACA CAGAAAGCTG GAAGCTGATG 
AGCACAGATG ATCAACAGAA AATCCAAGCA GCTGCATTTG ACAAAGGTGA TGACCGAAGA 
CTTGGCAAAA AGCCTATATT CAGTAGCTCT CAGCAAAGAA AACAGGTTTC TGACTGTGGT 
GCTATAAAAA ACAAATCCGC TGGAGGAAGT AACAAGAAAC AGTGTCTGAG TTATCTCTCT 
GCTCATCAGA AGATGAAATC TGATGGATTA GGAGCCTCTG GACATTCATC AAGTACTAAT 
AGAAATTCTA TAAATAAAAC TTTGAAGCAT GATGATTTAA AGGAAAAGGA TCCAAAACCA 
GCATCCAAGA GTACAAAAGA ATTAAAAACT GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA 
GCTATCATAA AGCCCAAAAC AGAAAATAGT GATAATGCAA AGTCAACAAA CCTGTCACCT 
AGACAAGCTG TGGAAAGATC AGCAACAGCA GCAGCAAATG AACAGAAAAA TTCAGGAAAT 
GGAAAAGGAG TGAGAAATCA GGAAGAGCAA ATTACAGGTG CCAGACCCAA GGTACTCACT 
GGAAATTTAA ATGTGCAAGC CAAAGCAAAG CCTTTGAAGA AAGTGACAGG CAAAGATTCT 
CCACACCTCC GAATGGCAGG GCCCTCCAGC AGATCTACAA ATTCAAGTAT GGAATTACTG 
ACTTCCACTG ACTCTCTAGA TGAACCAAAA GAAAATGGGT CAATGAAAGA GAAGCTTCCT 
GGTCATAAAC CTTCCTCTTG TGACTCTCGA GGACAGACTG TGAAAAACAG TGTAGAAGGT 
ATCAAAACTT CCACTGTAGC AATGAAATAT CGACCTGTTT CAAAAGATAC CAATGGAATT 
TCCAATAAAA AAAGCACTCA TGAACAGGAA TCTAATACAC ATAACAGTGT GCTAAAGAAG 
ATCACCAGCA AAGGATACAG TGATCCAGTA CCACAGGCAA TTTTAAAAAA AAGGGGAAAT 
GGCAATGGAT GTGCTACAGC TCCGCAGAGG ACAAAGAATG CCACAGCTAA TCTTGCTAAA 
ACTCAAGGAT CCCAAGGATC ACCAAATTCA GTAAAATCTT CAGTCTCTTC AAGGCAATCT 
GATGAAAATG TGACAAAATT GGACCACAGT GTAACTACAG ATAAACAGAC GCCTAAGAGA 
AAAATTGTCA AGCAAGGACA AACAACTTTG CCTAAGATTG GTGCAAAAAA GGTCACAATG 
CCTAAAAACC CAAATCAACC CAAAAGAGGT GAAAATTTGA ATAATAAAGA TGCAAAACCG 
AAAACACTTC CTGAACAGGT TATACTGAAG ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA 
AGTGAACCAT CTGTTGTCCA AAAAAGTGTG TCTCTTGATG TGTGTGACAA TAATAACAAA 
GGCAGTGTTT CTGAACAGAA GCCTCATGAA CCTCTAACTA ATCTCACAGC CAAAACCAGT 
GATGCAGAAG CATTTCAGTC ACCATGCATA CTCGACTCAC AGAAGACACT GAACAATCAA 
GAAAAACAGA AGTTGGTGTT AGAATGCCAA AATATTTCAA ATCTGGATAA ATTAATAAAA 
CGTGAACTGG AATCAAAACA GATCTGTTCA GTTAAAAGTG AAACAAATGT TTCTGGCCAC 
AAAAAAATAG ATCACTGCAC CACAGCTAAA ATACCTTGTC ATTCTGATGA GACTGATAAT 
GTCGATCCAA AATTTTATAG TACCACTGCT CTAAAATCCA TGATTTCAAA TCCTAAAGAA 
AACTCTTTGA ACTCTAATCC AGTTTGTGAC TTAGACTCAG CACACGTAGA ACAACTCCAT 
TCAGAATTAG ATAGGAAGAA GCAAGTAGGG AGAAAAGATA CAAACCAAAA ATTAAGCATT 
AAATGTGTGA AAGATGTTTT ACCTTGGGTT CCTGAAAAGA CAAATGGTAC CTTAAATTCT 
GGTCAAGATG ACAGAAAATC TAAAATTCAT GTAGAAGAAC AGACAGTTCC TAGTCAGTTG 
TCTGATGACT CTGCCATGAA TGAAGACAAT CATGCTACAG TAGAGTCACA TATTTCCTCC 
AAGTGTTTTT TGGAACAAAT ATCAGGGAAA AATTCTAAAG ATACGGAAAC AACAGAAACT 
TCAGAGAGCC ACGAAACTCC AGAAGCACCA TTCATGAGTC ACTGGAATTT GAGTACCAAT 
GTTCTGCATC AGAGAGAGAG TCCTGAATCA GACAGTGGCA GTGTCACAAC ATCCTCTGAT 
GACATAAAGC CCAGATCTGA AGACTATGAT GCTGGAGGGT CTCAGGATGA TGATGGGTCA 
AATGACAGAG GGGTTTCTAA ATGTGGCACT ATGCTGTGCC ATGATTTTCT TGGAAGGAGC 
AGCAGTGATA CCAGTACTCC TGAAGAATTA AAAATATATG ATAGCAACTT AAGAATTGAA 
GTGAAAATGA AAAAGCAAGG TAGTAGTGAT CTTTTCCAAG TTAATTCAAC AAGTGATGAT 
GAGATTCCCA GGAAAAGGCC AGAAATTTGG TCTCGATCTA CAATAGTTCA TCCTAGGGAA 
AAAGAAAATA TTCCACGAGG CAGTGTCCAG TTTGTACAGG AGGTAGATCA GGTTTCTTCC 
TCAGCAGATG AAACAGAAGA TGAAAGGTCT GAAGCTGAAA ATGTTGCAGA AAATTTCTCT 
ACATCTAACC CAGCTCTTCA GCAGTTTCAG GGGATAATTA ATCTAGCTTT TGAAGATGCA 
ACTGAAAATG AAAGTCATGA GTTTCGTGTA ACTAAAAGTT TTAAAAGGTC TGTTTTACTT 
TCCGTAGATG AATGTGAAGA GATAGGATCT GATGAAGGGG AGGTCCATGC TCTCTTTCAA 
CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT GTTTTTGATG GCATTTCTCA TGAACATCAT 
GGAAGGACCT GCTACTCCAG ATACTCACAT GGAAGTGAAG GTGGTGTTTT AGAATGCAGA 
CAAAAGAAAA GCAATACTAT ATATAAAAAC AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT 
GACTCATCAA GAAAAGATAA ACAGAGTGCT TCAGCCACAG AAAAAAAGGG TACAACAGAT 
GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT CCAGGAGATA AAGTATGTAG TGGAAGCAAT 
GTGGATAATG ACTTTGAGAC ACACAGCAAA TTTTCAGATA GTGATATAAA ATCTCAAGAA 
AGACCATGTC ATTTGGAACT TCGTCAAAGA GAATCCAATT CCGACATACC AAAGAACAGC 
TTCACAAAGT CTCTGGACTC CTGTCGGAGT CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA 
GAAAGCCATT CTACAGCTAC CGAAAAAGCT AATATTGCTT TATCTGCAGG AGACATAGAT 
GGTTGTGACA CAGTGGCACA AATCTACATG TATGACCATC GGCCTTCAAA AACCCTGTCT 
CCAATATATG AGATGGATGT AACAGAAGCA TTTGAGCAGA AAATGGAATC AGAAACACAA 
GGTACAGACA TGGATTTTGA AGATGAGCAA CACTTTGCAA AACAAGACTG GACACTATTA 
AAGCAACTGC TCTCTGAACA GGACTCTAAC TTAAATATCA CAAATTCTGT TCCTGAAGAC 
TTAAATTTAG CACAGTATCT AATTAATCAG ACACTACTTT TAGCACGAGA CAGCTCAAAA 
CCTCAGGGTA AAGCACATGT AGACACTTTG AGCAGATGGA GTGAACTAAC ATCTCCATTT 
GATGATTCCT CAGCAAGCAT TACCATGGCT AGTTTTTCCT CTGAAGAATG TTCACCCCAA 
GGGGAATGGA CAATTCTGGA ACTGGAAACT CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801607.1
CDS	248..5506
Translation	MARCGEDSASPGVPLGSPGVCSKGLQRKGPCERRRLKAMVSEQLSQDLLRLLREEVHTDVTFSIGCTLFKAHKAVLLARVPDFYFHTVGHTDNLKNHEHVTVENFEASEFRTFLQIVYSSNRNIKSYEEEILRKKIVESGVPQKKCDFSVGNCTDHDRRLSVEFLSKRSSDHSLLKHEIPKDINSKEENVISTDIYDLEPASELGEDLLKLYVKHCCPDIDIYVDGKSFKAHRAILSARSSYFAAMLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803305.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803305.2

ORF Insert Sequence:

ATGGCCCGCT GTGGGGAGGA TAGCGCGTCC CCCGGGGTAC CTCTGGGATC CCCCGGAGTT 
TGCAGTAAGG GGTTGCAAAG GAAGGGACCC TGTGAGCGGC GCCGGCTGAA GGCGATGGTG 
TCGGAGCAGC TCAGCCAGGA TCTGCTCAGG CTTCTAAGAG AAGAAGTACA CACAGATGTT 
ACTTTCTCTA TAGGTTGTAC TTTGTTCAAA GCACACAAAG CAGTCCTTTT AGCAAGGGTT 
CCTGATTTCT ATTTTCATAC TGTTGGACAT ACAGATAACT TAAAAAATCA TGAACATGTC 
ACTGTGGAGA ACTTTGAAGC TTCAGAATTT AGAACATTTT TACAGATTGT ATACTCATCA 
AACAGAAATA TAAAAAGCTA CGAAGAAGAA ATTCTTAGGA AAAAGATAGT GGAAAGTGGA 
GTACCACAAA AGAAATGTGA CTTCAGTGTT GGAAATTGTA CTGACCATGA TAGAAGGTTA 
TCTGTAGAGT TCTTAAGTAA AAGATCATCT GATCATTCTC TTCTGAAACA TGAAATTCCA 
AAAGATATCA ACAGCAAAGA GGAAAATGTA ATTTCCACTG ATATTTATGA CTTGGAACCT 
GCATCTGAAT TAGGAGAAGA TTTACTGAAG CTTTATGTGA AACACTGTTG CCCAGATATT 
GATATTTATG TTGACGGAAA AAGTTTTAAA GCTCACAGAG CCATTTTAAG TGCCAGATCT 
AGTTATTTTG CTGCAATGCT GAGTGGCTGT TGGGCTGAAA GCTCCCAGGA GTGCGTTACT 
CTCCAAAGTA TAAGCCATGT AGAAATGAAT GTTATGATGC ACTTTATATA CGGAGGAACT 
TTGGACTTTC CAGTCAAAGC TAATGTTGGT CAGATACTCA ATATGGCTGA TATGTATGGA 
CTAGAAGGAT TAAAAGAAGT AGCAATCTAT ATTTTAAGAA GAGATTACTG TAATTTCTTT 
CAGAAGCCTG TTTCCAGAAG GTTGGCATCT ATATTAGAAT GCCTGATTAT TGCTCATTCA 
GTTGGAGTGG AAAGTCTTTT TGCTGACTGC ATGAAGTGGA TTGTAAAGCA CTTTGCAAGG 
TTTTGGTCTG AAAGAAGCTT TGCAAATGTA CCTCCTGAGA TTCAGAAAAG TTGTTTTAAT 
ATGTTGATTC ATTCTTTAAA TGATAAGAAT GCTGCTTTTC TTCTGATGGA AAGTGACAGG 
CTAATCGTCA GTTTACCCAG AGTGAAGTGG ACAGAGTCAG CATTGATCAT GGCATCTCAG 
CTCCAAGAAG AATGCATTGC ATTTATTATA GAAAATTTCT CCAAGATCAT TCAAAGTGAA 
AATTTTGCTC TTCTGTTACA GGGTTTTACG TGCAAGATCC AGGCTCTGCG TGATAAGCTG 
TGGATCTTCC TGGTTCAGTC TTTCTATGCT GTTCGTCACA CAGAAAGCTG GAAGCTGATG 
AGCACAGATG ATCAACAGAA AATCCAAGCA GCTGCATTTG ACAAAGGTGA TGACCGAAGA 
CTTGGCAAAA AGCCTATATT CAGTAGCTCT CAGCAAAGAA AACAGGTTTC TGACTGTGGT 
GCTATAAAAA ACAAATCCGC TGGAGGAAGT AACAAGAAAC AGTGTCTGAG TTATCTCTCT 
GCTCATCAGA AGATGAAATC TGATGGATTA GGAGCCTCTG GACATTCATC AAGTACTAAT 
AGAAATTCTA TAAATAAAAC TTTGAAGCAT GATGATTTAA AGGAAAAGGA TCCAAAACCA 
GCATCCAAGA GTACAAAAGA ATTAAAAACT GTGGAAAAAA ATGTTTCTGG AAAGCCCAAA 
GCTATCATAA AGCCCAAAAC AGAAAATAGT GATAATGCAA AGTCAACAAA CCTGTCACCT 
AGACAAGCTG TGGAAAGATC AGCAACAGCA GCAGCAAATG AACAGAAAAA TTCAGGAAAT 
GGAAAAGGAG TGAGAAATCA GGAAGAGCAA ATTACAGGTG CCAGACCCAA GGTACTCACT 
GGAAATTTAA ATGTGCAAGC CAAAGCAAAG CCTTTGAAGA AAGTGACAGG CAAAGATTCT 
CCACACCTCC GAATGGCAGG GCCCTCCAGC AGATCTACAA ATTCAAGTAT GGAATTACTG 
ACTTCCACTG ACTCTCTAGA TGAACCAAAA GAAAATGGGT CAATGAAAGA GAAGCTTCCT 
GGTCATAAAC CTTCCTCTTG TGACTCTCGA GGACAGACTG TGAAAAACAG TGTAGAAGGT 
ATCAAAACTT CCACTGTAGC AATGAAATAT CGACCTGTTT CAAAAGATAC CAATGGAATT 
TCCAATAAAA AAAGCACTCA TGAACAGGAA TCTAATACAC ATAACAGTGT GCTAAAGAAG 
ATCACCAGCA AAGGATACAG TGATCCAGTA CCACAGGCAA TTTTAAAAAA AAGGGGAAAT 
GGCAATGGAT GTGCTACAGC TCCGCAGAGG ACAAAGAATG CCACAGCTAA TCTTGCTAAA 
ACTCAAGGAT CCCAAGGATC ACCAAATTCA GTAAAATCTT CAGTCTCTTC AAGGCAATCT 
GATGAAAATG TGACAAAATT GGACCACAGT GTAACTACAG ATAAACAGAC GCCTAAGAGA 
AAAATTGTCA AGCAAGGACA AACAACTTTG CCTAAGATTG GTGCAAAAAA GGTCACAATG 
CCTAAAAACC CAAATCAACC CAAAAGAGGT GAAAATTTGA ATAATAAAGA TGCAAAACCG 
AAAACACTTC CTGAACAGGT TATACTGAAG ACTCTGCCTT CTTCTCAGAG ACCTTTAAAA 
AGTGAACCAT CTGTTGTCCA AAAAAGTGTG TCTCTTGATG TGTGTGACAA TAATAACAAA 
GGCAGTGTTT CTGAACAGAA GCCTCATGAA CCTCTAACTA ATCTCACAGC CAAAACCAGT 
GATGCAGAAG CATTTCAGTC ACCATGCATA CTCGACTCAC AGAAGACACT GAACAATCAA 
GAAAAACAGA AGTTGGTGTT AGAATGCCAA AATATTTCAA ATCTGGATAA ATTAATAAAA 
CGTGAACTGG AATCAAAACA GATCTGTTCA GTTAAAAGTG AAACAAATGT TTCTGGCCAC 
AAAAAAATAG ATCACTGCAC CACAGCTAAA ATACCTTGTC ATTCTGATGA GACTGATAAT 
GTCGATCCAA AATTTTATAG TACCACTGCT CTAAAATCCA TGATTTCAAA TCCTAAAGAA 
AACTCTTTGA ACTCTAATCC AGTTTGTGAC TTAGACTCAG CACACGTAGA ACAACTCCAT 
TCAGAATTAG ATAGGAAGAA GCAAGTAGGG AGAAAAGATA CAAACCAAAA ATTAAGCATT 
AAATGTGTGA AAGATGTTTT ACCTTGGGTT CCTGAAAAGA CAAATGGTAC CTTAAATTCT 
GGTCAAGATG ACAGAAAATC TAAAATTCAT GTAGAAGAAC AGACAGTTCC TAGTCAGTTG 
TCTGATGACT CTGCCATGAA TGAAGACAAT CATGCTACAG TAGAGTCACA TATTTCCTCC 
AAGTGTTTTT TGGAACAAAT ATCAGGGAAA AATTCTAAAG ATACGGAAAC AACAGAAACT 
TCAGAGAGCC ACGAAACTCC AGAAGCACCA TTCATGAGTC ACTGGAATTT GAGTACCAAT 
GTTCTGCATC AGAGAGAGAG TCCTGAATCA GACAGTGGCA GTGTCACAAC ATCCTCTGAT 
GACATAAAGC CCAGATCTGA AGACTATGAT GCTGGAGGGT CTCAGGATGA TGATGGGTCA 
AATGACAGAG GGGTTTCTAA ATGTGGCACT ATGCTGTGCC ATGATTTTCT TGGAAGGAGC 
AGCAGTGATA CCAGTACTCC TGAAGAATTA AAAATATATG ATAGCAACTT AAGAATTGAA 
GTGAAAATGA AAAAGCAAGG TAGTAGTGAT CTTTTCCAAG TTAATTCAAC AAGTGATGAT 
GAGATTCCCA GGAAAAGGCC AGAAATTTGG TCTCGATCTA CAATAGTTCA TCCTAGGGAA 
AAAGAAAATA TTCCACGAGG CAGTGTCCAG TTTGTACAGG AGGTAGATCA GGTTTCTTCC 
TCAGCAGATG AAACAGAAGA TGAAAGGTCT GAAGCTGAAA ATGTTGCAGA AAATTTCTCT 
ACATCTAACC CAGCTCTTCA GCAGTTTCAG GGGATAATTA ATCTAGCTTT TGAAGATGCA 
ACTGAAAATG AAAGTCATGA GTTTCGTGTA ACTAAAAGTT TTAAAAGGTC TGTTTTACTT 
TCCGTAGATG AATGTGAAGA GATAGGATCT GATGAAGGGG AGGTCCATGC TCTCTTTCAA 
CCTTCTGTAG ATTCTCTCTC ACCTTCTGAT GTTTTTGATG GCATTTCTCA TGAACATCAT 
GGAAGGACCT GCTACTCCAG ATACTCACAT GGAAGTGAAG GTGGTGTTTT AGAATGCAGA 
CAAAAGAAAA GCAATACTAT ATATAAAAAC AAAAGCTTTC CCTTGGGTCT TAGTAGCGTT 
GACTCATCAA GAAAAGATAA ACAGAGTGCT TCAGCCACAG AAAAAAAGGG TACAACAGAT 
GTCCTGTCCA AAGGAGGCAT ACAGCTTCCT CCAGGAGATA AAGTATGTAG TGGAAGCAAT 
GTGGATAATG ACTTTGAGAC ACACAGCAAA TTTTCAGATA GTGATATAAA ATCTCAAGAA 
AGACCATGTC ATTTGGAACT TCGTCAAAGA GAATCCAATT CCGACATACC AAAGAACAGC 
TTCACAAAGT CTCTGGACTC CTGTCGGAGT CAAGTTCTGC CTCAGGAAGG TCAAGTGAAA 
GAAAGCCATT CTACAGCTAC CGAAAAAGCT AATATTGCTT TATCTGCAGG AGACATAGAT 
GGTTGTGACA CAGTGGCACA AATCTACATG TATGACCATC GGCCTTCAAA AACCCTGTCT 
CCAATATATG AGATGGATGT AACAGAAGCA TTTGAGCAGA AAATGGAATC AGAAACACAA 
GGTACAGACA TGGATTTTGA AGATGAGCAA CACTTTGCAA AACAAGACTG GACACTATTA 
AAGCAACTGC TCTCTGAACA GGACTCTAAC TTAAATATCA CAAATTCTGT TCCTGAAGAC 
TTAAATTTAG CACAGTATCT AATTAATCAG ACACTACTTT TAGCACGAGA CAGCTCAAAA 
CCTCAGGGTA AAGCACATGT AGACACTTTG AGCAGATGGA GTGAACTAAC ATCTCCATTT 
GATGATTCCT CAGCAAGCAT TACCATGGCT AGTTTTTCCT CTGAAGAATG TTCACCCCAA 
GGGGAATGGA CAATTCTGGA ACTGGAAACT CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_024989037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844805.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X6, mRNA.

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CloneID	OBa50259
Clone ID Related Accession (Same CDS sequence)	XM_024989037.1 , XM_024989033.1 , XM_024989028.1 , XM_010803308.3 , XM_010803308.2
Accession Version	XM_024989037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844805.1
CDS	267..4928
Translation	MLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_024989037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989037.1

ORF Insert Sequence:

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_024989033.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844801.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X5, mRNA.

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CloneID	OBa50259
Clone ID Related Accession (Same CDS sequence)	XM_024989037.1 , XM_024989033.1 , XM_024989028.1 , XM_010803308.3 , XM_010803308.2
Accession Version	XM_024989033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844801.1
CDS	416..5077
Translation	MLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_024989033.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989033.1

ORF Insert Sequence:

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_024989028.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844796.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.

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CloneID	OBa50259
Clone ID Related Accession (Same CDS sequence)	XM_024989037.1 , XM_024989033.1 , XM_024989028.1 , XM_010803308.3 , XM_010803308.2
Accession Version	XM_024989028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844796.1
CDS	291..4952
Translation	MLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_024989028.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989028.1

ORF Insert Sequence:

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803308.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801610.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa50259
Clone ID Related Accession (Same CDS sequence)	XM_024989037.1 , XM_024989033.1 , XM_024989028.1 , XM_010803308.3 , XM_010803308.2
Accession Version	XM_010803308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801610.1
CDS	140..4801
Translation	MLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803308.3
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803308.3

ORF Insert Sequence:

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803308.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801610.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa50259
Clone ID Related Accession (Same CDS sequence)	XM_024989037.1 , XM_024989033.1 , XM_024989028.1 , XM_010803308.3 , XM_010803308.2
Accession Version	XM_010803308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801610.1
CDS	119..4780
Translation	MLSGCWAESSQECVTLQSISHVEMNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803308.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803308.2

ORF Insert Sequence:

ATGCTGAGTG GCTGTTGGGC TGAAAGCTCC CAGGAGTGCG TTACTCTCCA AAGTATAAGC 
CATGTAGAAA TGAATGTTAT GATGCACTTT ATATACGGAG GAACTTTGGA CTTTCCAGTC 
AAAGCTAATG TTGGTCAGAT ACTCAATATG GCTGATATGT ATGGACTAGA AGGATTAAAA 
GAAGTAGCAA TCTATATTTT AAGAAGAGAT TACTGTAATT TCTTTCAGAA GCCTGTTTCC 
AGAAGGTTGG CATCTATATT AGAATGCCTG ATTATTGCTC ATTCAGTTGG AGTGGAAAGT 
CTTTTTGCTG ACTGCATGAA GTGGATTGTA AAGCACTTTG CAAGGTTTTG GTCTGAAAGA 
AGCTTTGCAA ATGTACCTCC TGAGATTCAG AAAAGTTGTT TTAATATGTT GATTCATTCT 
TTAAATGATA AGAATGCTGC TTTTCTTCTG ATGGAAAGTG ACAGGCTAAT CGTCAGTTTA 
CCCAGAGTGA AGTGGACAGA GTCAGCATTG ATCATGGCAT CTCAGCTCCA AGAAGAATGC 
ATTGCATTTA TTATAGAAAA TTTCTCCAAG ATCATTCAAA GTGAAAATTT TGCTCTTCTG 
TTACAGTCAC AAGCAATGAG TAGCACATCT GATCTGTTGG ACAAAATTTT TAAAGCTGTT 
GAAGAAAACA TTACCACTGA GAATAGTTGC TCGCTTCTTA TGGCTCTGGA CACATTACTG 
AACTCTGACA GTACAAAAGA AATGGGTTTT ACGTGCAAGA TCCAGGCTCT GCGTGATAAG 
CTGTGGATCT TCCTGGTTCA GTCTTTCTAT GCTGTTCGTC ACACAGAAAG CTGGAAGCTG 
ATGAGCACAG ATGATCAACA GAAAATCCAA GCAGCTGCAT TTGACAAAGG TGATGACCGA 
AGACTTGGCA AAAAGCCTAT ATTCAGTAGC TCTCAGCAAA GAAAACAGGT TTCTGACTGT 
GGTGCTATAA AAAACAAATC CGCTGGAGGA AGTAACAAGA AACAGTGTCT GAGTTATCTC 
TCTGCTCATC AGAAGATGAA ATCTGATGGA TTAGGAGCCT CTGGACATTC ATCAAGTACT 
AATAGAAATT CTATAAATAA AACTTTGAAG CATGATGATT TAAAGGAAAA GGATCCAAAA 
CCAGCATCCA AGAGTACAAA AGAATTAAAA ACTGTGGAAA AAAATGTTTC TGGAAAGCCC 
AAAGCTATCA TAAAGCCCAA AACAGAAAAT AGTGATAATG CAAAGTCAAC AAACCTGTCA 
CCTAGACAAG CTGTGGAAAG ATCAGCAACA GCAGCAGCAA ATGAACAGAA AAATTCAGGA 
AATGGAAAAG GAGTGAGAAA TCAGGAAGAG CAAATTACAG GTGCCAGACC CAAGGTACTC 
ACTGGAAATT TAAATGTGCA AGCCAAAGCA AAGCCTTTGA AGAAAGTGAC AGGCAAAGAT 
TCTCCACACC TCCGAATGGC AGGGCCCTCC AGCAGATCTA CAAATTCAAG TATGGAATTA 
CTGACTTCCA CTGACTCTCT AGATGAACCA AAAGAAAATG GGTCAATGAA AGAGAAGCTT 
CCTGGTCATA AACCTTCCTC TTGTGACTCT CGAGGACAGA CTGTGAAAAA CAGTGTAGAA 
GGTATCAAAA CTTCCACTGT AGCAATGAAA TATCGACCTG TTTCAAAAGA TACCAATGGA 
ATTTCCAATA AAAAAAGCAC TCATGAACAG GAATCTAATA CACATAACAG TGTGCTAAAG 
AAGATCACCA GCAAAGGATA CAGTGATCCA GTACCACAGG CAATTTTAAA AAAAAGGGGA 
AATGGCAATG GATGTGCTAC AGCTCCGCAG AGGACAAAGA ATGCCACAGC TAATCTTGCT 
AAAACTCAAG GATCCCAAGG ATCACCAAAT TCAGTAAAAT CTTCAGTCTC TTCAAGGCAA 
TCTGATGAAA ATGTGACAAA ATTGGACCAC AGTGTAACTA CAGATAAACA GACGCCTAAG 
AGAAAAATTG TCAAGCAAGG ACAAACAACT TTGCCTAAGA TTGGTGCAAA AAAGGTCACA 
ATGCCTAAAA ACCCAAATCA ACCCAAAAGA GGTGAAAATT TGAATAATAA AGATGCAAAA 
CCGAAAACAC TTCCTGAACA GGTTATACTG AAGACTCTGC CTTCTTCTCA GAGACCTTTA 
AAAAGTGAAC CATCTGTTGT CCAAAAAAGT GTGTCTCTTG ATGTGTGTGA CAATAATAAC 
AAAGGCAGTG TTTCTGAACA GAAGCCTCAT GAACCTCTAA CTAATCTCAC AGCCAAAACC 
AGTGATGCAG AAGCATTTCA GTCACCATGC ATACTCGACT CACAGAAGAC ACTGAACAAT 
CAAGAAAAAC AGAAGTTGGT GTTAGAATGC CAAAATATTT CAAATCTGGA TAAATTAATA 
AAACGTGAAC TGGAATCAAA ACAGATCTGT TCAGTTAAAA GTGAAACAAA TGTTTCTGGC 
CACAAAAAAA TAGATCACTG CACCACAGCT AAAATACCTT GTCATTCTGA TGAGACTGAT 
AATGTCGATC CAAAATTTTA TAGTACCACT GCTCTAAAAT CCATGATTTC AAATCCTAAA 
GAAAACTCTT TGAACTCTAA TCCAGTTTGT GACTTAGACT CAGCACACGT AGAACAACTC 
CATTCAGAAT TAGATAGGAA GAAGCAAGTA GGGAGAAAAG ATACAAACCA AAAATTAAGC 
ATTAAATGTG TGAAAGATGT TTTACCTTGG GTTCCTGAAA AGACAAATGG TACCTTAAAT 
TCTGGTCAAG ATGACAGAAA ATCTAAAATT CATGTAGAAG AACAGACAGT TCCTAGTCAG 
TTGTCTGATG ACTCTGCCAT GAATGAAGAC AATCATGCTA CAGTAGAGTC ACATATTTCC 
TCCAAGTGTT TTTTGGAACA AATATCAGGG AAAAATTCTA AAGATACGGA AACAACAGAA 
ACTTCAGAGA GCCACGAAAC TCCAGAAGCA CCATTCATGA GTCACTGGAA TTTGAGTACC 
AATGTTCTGC ATCAGAGAGA GAGTCCTGAA TCAGACAGTG GCAGTGTCAC AACATCCTCT 
GATGACATAA AGCCCAGATC TGAAGACTAT GATGCTGGAG GGTCTCAGGA TGATGATGGG 
TCAAATGACA GAGGGGTTTC TAAATGTGGC ACTATGCTGT GCCATGATTT TCTTGGAAGG 
AGCAGCAGTG ATACCAGTAC TCCTGAAGAA TTAAAAATAT ATGATAGCAA CTTAAGAATT 
GAAGTGAAAA TGAAAAAGCA AGGTAGTAGT GATCTTTTCC AAGTTAATTC AACAAGTGAT 
GATGAGATTC CCAGGAAAAG GCCAGAAATT TGGTCTCGAT CTACAATAGT TCATCCTAGG 
GAAAAAGAAA ATATTCCACG AGGCAGTGTC CAGTTTGTAC AGGAGGTAGA TCAGGTTTCT 
TCCTCAGCAG ATGAAACAGA AGATGAAAGG TCTGAAGCTG AAAATGTTGC AGAAAATTTC 
TCTACATCTA ACCCAGCTCT TCAGCAGTTT CAGGGGATAA TTAATCTAGC TTTTGAAGAT 
GCAACTGAAA ATGAAAGTCA TGAGTTTCGT GTAACTAAAA GTTTTAAAAG GTCTGTTTTA 
CTTTCCGTAG ATGAATGTGA AGAGATAGGA TCTGATGAAG GGGAGGTCCA TGCTCTCTTT 
CAACCTTCTG TAGATTCTCT CTCACCTTCT GATGTTTTTG ATGGCATTTC TCATGAACAT 
CATGGAAGGA CCTGCTACTC CAGATACTCA CATGGAAGTG AAGGTGGTGT TTTAGAATGC 
AGACAAAAGA AAAGCAATAC TATATATAAA AACAAAAGCT TTCCCTTGGG TCTTAGTAGC 
GTTGACTCAT CAAGAAAAGA TAAACAGAGT GCTTCAGCCA CAGAAAAAAA GGGTACAACA 
GATGTCCTGT CCAAAGGAGG CATACAGCTT CCTCCAGGAG ATAAAGTATG TAGTGGAAGC 
AATGTGGATA ATGACTTTGA GACACACAGC AAATTTTCAG ATAGTGATAT AAAATCTCAA 
GAAAGACCAT GTCATTTGGA ACTTCGTCAA AGAGAATCCA ATTCCGACAT ACCAAAGAAC 
AGCTTCACAA AGTCTCTGGA CTCCTGTCGG AGTCAAGTTC TGCCTCAGGA AGGTCAAGTG 
AAAGAAAGCC ATTCTACAGC TACCGAAAAA GCTAATATTG CTTTATCTGC AGGAGACATA 
GATGGTTGTG ACACAGTGGC ACAAATCTAC ATGTATGACC ATCGGCCTTC AAAAACCCTG 
TCTCCAATAT ATGAGATGGA TGTAACAGAA GCATTTGAGC AGAAAATGGA ATCAGAAACA 
CAAGGTACAG ACATGGATTT TGAAGATGAG CAACACTTTG CAAAACAAGA CTGGACACTA 
TTAAAGCAAC TGCTCTCTGA ACAGGACTCT AACTTAAATA TCACAAATTC TGTTCCTGAA 
GACTTAAATT TAGCACAGTA TCTAATTAAT CAGACACTAC TTTTAGCACG AGACAGCTCA 
AAACCTCAGG GTAAAGCACA TGTAGACACT TTGAGCAGAT GGAGTGAACT AACATCTCCA 
TTTGATGATT CCTCAGCAAG CATTACCATG GCTAGTTTTT CCTCTGAAGA ATGTTCACCC 
CAAGGGGAAT GGACAATTCT GGAACTGGAA ACTCAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803309.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801611.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X8, mRNA.

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CloneID	OBa50260
Clone ID Related Accession (Same CDS sequence)	XM_010803309.3 , XM_010803309.2
Accession Version	XM_010803309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTA TGATGCACTT TATATACGGA GGAACTTTGG ACTTTCCAGT CAAAGCTAAT 
GTTGGTCAGA TACTCAATAT GGCTGATATG TATGGACTAG AAGGATTAAA AGAAGTAGCA 
ATCTATATTT TAAGAAGAGA TTACTGTAAT TTCTTTCAGA AGCCTGTTTC CAGAAGGTTG 
GCATCTATAT TAGAATGCCT GATTATTGCT CATTCAGTTG GAGTGGAAAG TCTTTTTGCT 
GACTGCATGA AGTGGATTGT AAAGCACTTT GCAAGGTTTT GGTCTGAAAG AAGCTTTGCA 
AATGTACCTC CTGAGATTCA GAAAAGTTGT TTTAATATGT TGATTCATTC TTTAAATGAT 
AAGAATGCTG CTTTTCTTCT GATGGAAAGT GACAGGCTAA TCGTCAGTTT ACCCAGAGTG 
AAGTGGACAG AGTCAGCATT GATCATGGCA TCTCAGCTCC AAGAAGAATG CATTGCATTT 
ATTATAGAAA ATTTCTCCAA GATCATTCAA AGTGAAAATT TTGCTCTTCT GTTACAGTCA 
CAAGCAATGA GTAGCACATC TGATCTGTTG GACAAAATTT TTAAAGCTGT TGAAGAAAAC 
ATTACCACTG AGAATAGTTG CTCGCTTCTT ATGGCTCTGG ACACATTACT GAACTCTGAC 
AGTACAAAAG AAATGGGTTT TACGTGCAAG ATCCAGGCTC TGCGTGATAA GCTGTGGATC 
TTCCTGGTTC AGTCTTTCTA TGCTGTTCGT CACACAGAAA GCTGGAAGCT GATGAGCACA 
GATGATCAAC AGAAAATCCA AGCAGCTGCA TTTGACAAAG GTGATGACCG AAGACTTGGC 
AAAAAGCCTA TATTCAGTAG CTCTCAGCAA AGAAAACAGG TTTCTGACTG TGGTGCTATA 
AAAAACAAAT CCGCTGGAGG AAGTAACAAG AAACAGTGTC TGAGTTATCT CTCTGCTCAT 
CAGAAGATGA AATCTGATGG ATTAGGAGCC TCTGGACATT CATCAAGTAC TAATAGAAAT 
TCTATAAATA AAACTTTGAA GCATGATGAT TTAAAGGAAA AGGATCCAAA ACCAGCATCC 
AAGAGTACAA AAGAATTAAA AACTGTGGAA AAAAATGTTT CTGGAAAGCC CAAAGCTATC 
ATAAAGCCCA AAACAGAAAA TAGTGATAAT GCAAAGTCAA CAAACCTGTC ACCTAGACAA 
GCTGTGGAAA GATCAGCAAC AGCAGCAGCA AATGAACAGA AAAATTCAGG AAATGGAAAA 
GGAGTGAGAA ATCAGGAAGA GCAAATTACA GGTGCCAGAC CCAAGGTACT CACTGGAAAT 
TTAAATGTGC AAGCCAAAGC AAAGCCTTTG AAGAAAGTGA CAGGCAAAGA TTCTCCACAC 
CTCCGAATGG CAGGGCCCTC CAGCAGATCT ACAAATTCAA GTATGGAATT ACTGACTTCC 
ACTGACTCTC TAGATGAACC AAAAGAAAAT GGGTCAATGA AAGAGAAGCT TCCTGGTCAT 
AAACCTTCCT CTTGTGACTC TCGAGGACAG ACTGTGAAAA ACAGTGTAGA AGGTATCAAA 
ACTTCCACTG TAGCAATGAA ATATCGACCT GTTTCAAAAG ATACCAATGG AATTTCCAAT 
AAAAAAAGCA CTCATGAACA GGAATCTAAT ACACATAACA GTGTGCTAAA GAAGATCACC 
AGCAAAGGAT ACAGTGATCC AGTACCACAG GCAATTTTAA AAAAAAGGGG AAATGGCAAT 
GGATGTGCTA CAGCTCCGCA GAGGACAAAG AATGCCACAG CTAATCTTGC TAAAACTCAA 
GGATCCCAAG GATCACCAAA TTCAGTAAAA TCTTCAGTCT CTTCAAGGCA ATCTGATGAA 
AATGTGACAA AATTGGACCA CAGTGTAACT ACAGATAAAC AGACGCCTAA GAGAAAAATT 
GTCAAGCAAG GACAAACAAC TTTGCCTAAG ATTGGTGCAA AAAAGGTCAC AATGCCTAAA 
AACCCAAATC AACCCAAAAG AGGTGAAAAT TTGAATAATA AAGATGCAAA ACCGAAAACA 
CTTCCTGAAC AGGTTATACT GAAGACTCTG CCTTCTTCTC AGAGACCTTT AAAAAGTGAA 
CCATCTGTTG TCCAAAAAAG TGTGTCTCTT GATGTGTGTG ACAATAATAA CAAAGGCAGT 
GTTTCTGAAC AGAAGCCTCA TGAACCTCTA ACTAATCTCA CAGCCAAAAC CAGTGATGCA 
GAAGCATTTC AGTCACCATG CATACTCGAC TCACAGAAGA CACTGAACAA TCAAGAAAAA 
CAGAAGTTGG TGTTAGAATG CCAAAATATT TCAAATCTGG ATAAATTAAT AAAACGTGAA 
CTGGAATCAA AACAGATCTG TTCAGTTAAA AGTGAAACAA ATGTTTCTGG CCACAAAAAA 
ATAGATCACT GCACCACAGC TAAAATACCT TGTCATTCTG ATGAGACTGA TAATGTCGAT 
CCAAAATTTT ATAGTACCAC TGCTCTAAAA TCCATGATTT CAAATCCTAA AGAAAACTCT 
TTGAACTCTA ATCCAGTTTG TGACTTAGAC TCAGCACACG TAGAACAACT CCATTCAGAA 
TTAGATAGGA AGAAGCAAGT AGGGAGAAAA GATACAAACC AAAAATTAAG CATTAAATGT 
GTGAAAGATG TTTTACCTTG GGTTCCTGAA AAGACAAATG GTACCTTAAA TTCTGGTCAA 
GATGACAGAA AATCTAAAAT TCATGTAGAA GAACAGACAG TTCCTAGTCA GTTGTCTGAT 
GACTCTGCCA TGAATGAAGA CAATCATGCT ACAGTAGAGT CACATATTTC CTCCAAGTGT 
TTTTTGGAAC AAATATCAGG GAAAAATTCT AAAGATACGG AAACAACAGA AACTTCAGAG 
AGCCACGAAA CTCCAGAAGC ACCATTCATG AGTCACTGGA ATTTGAGTAC CAATGTTCTG 
CATCAGAGAG AGAGTCCTGA ATCAGACAGT GGCAGTGTCA CAACATCCTC TGATGACATA 
AAGCCCAGAT CTGAAGACTA TGATGCTGGA GGGTCTCAGG ATGATGATGG GTCAAATGAC 
AGAGGGGTTT CTAAATGTGG CACTATGCTG TGCCATGATT TTCTTGGAAG GAGCAGCAGT 
GATACCAGTA CTCCTGAAGA ATTAAAAATA TATGATAGCA ACTTAAGAAT TGAAGTGAAA 
ATGAAAAAGC AAGGTAGTAG TGATCTTTTC CAAGTTAATT CAACAAGTGA TGATGAGATT 
CCCAGGAAAA GGCCAGAAAT TTGGTCTCGA TCTACAATAG TTCATCCTAG GGAAAAAGAA 
AATATTCCAC GAGGCAGTGT CCAGTTTGTA CAGGAGGTAG ATCAGGTTTC TTCCTCAGCA 
GATGAAACAG AAGATGAAAG GTCTGAAGCT GAAAATGTTG CAGAAAATTT CTCTACATCT 
AACCCAGCTC TTCAGCAGTT TCAGGGGATA ATTAATCTAG CTTTTGAAGA TGCAACTGAA 
AATGAAAGTC ATGAGTTTCG TGTAACTAAA AGTTTTAAAA GGTCTGTTTT ACTTTCCGTA 
GATGAATGTG AAGAGATAGG ATCTGATGAA GGGGAGGTCC ATGCTCTCTT TCAACCTTCT 
GTAGATTCTC TCTCACCTTC TGATGTTTTT GATGGCATTT CTCATGAACA TCATGGAAGG 
ACCTGCTACT CCAGATACTC ACATGGAAGT GAAGGTGGTG TTTTAGAATG CAGACAAAAG 
AAAAGCAATA CTATATATAA AAACAAAAGC TTTCCCTTGG GTCTTAGTAG CGTTGACTCA 
TCAAGAAAAG ATAAACAGAG TGCTTCAGCC ACAGAAAAAA AGGGTACAAC AGATGTCCTG 
TCCAAAGGAG GCATACAGCT TCCTCCAGGA GATAAAGTAT GTAGTGGAAG CAATGTGGAT 
AATGACTTTG AGACACACAG CAAATTTTCA GATAGTGATA TAAAATCTCA AGAAAGACCA 
TGTCATTTGG AACTTCGTCA AAGAGAATCC AATTCCGACA TACCAAAGAA CAGCTTCACA 
AAGTCTCTGG ACTCCTGTCG GAGTCAAGTT CTGCCTCAGG AAGGTCAAGT GAAAGAAAGC 
CATTCTACAG CTACCGAAAA AGCTAATATT GCTTTATCTG CAGGAGACAT AGATGGTTGT 
GACACAGTGG CACAAATCTA CATGTATGAC CATCGGCCTT CAAAAACCCT GTCTCCAATA 
TATGAGATGG ATGTAACAGA AGCATTTGAG CAGAAAATGG AATCAGAAAC ACAAGGTACA 
GACATGGATT TTGAAGATGA GCAACACTTT GCAAAACAAG ACTGGACACT ATTAAAGCAA 
CTGCTCTCTG AACAGGACTC TAACTTAAAT ATCACAAATT CTGTTCCTGA AGACTTAAAT 
TTAGCACAGT ATCTAATTAA TCAGACACTA CTTTTAGCAC GAGACAGCTC AAAACCTCAG 
GGTAAAGCAC ATGTAGACAC TTTGAGCAGA TGGAGTGAAC TAACATCTCC ATTTGATGAT 
TCCTCAGCAA GCATTACCAT GGCTAGTTTT TCCTCTGAAG AATGTTCACC CCAAGGGGAA 
TGGACAATTC TGGAACTGGA AACTCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801611.1
CDS	146..4738
Translation	MNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803309.3
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803309.3

ORF Insert Sequence:

ATGAATGTTA TGATGCACTT TATATACGGA GGAACTTTGG ACTTTCCAGT CAAAGCTAAT 
GTTGGTCAGA TACTCAATAT GGCTGATATG TATGGACTAG AAGGATTAAA AGAAGTAGCA 
ATCTATATTT TAAGAAGAGA TTACTGTAAT TTCTTTCAGA AGCCTGTTTC CAGAAGGTTG 
GCATCTATAT TAGAATGCCT GATTATTGCT CATTCAGTTG GAGTGGAAAG TCTTTTTGCT 
GACTGCATGA AGTGGATTGT AAAGCACTTT GCAAGGTTTT GGTCTGAAAG AAGCTTTGCA 
AATGTACCTC CTGAGATTCA GAAAAGTTGT TTTAATATGT TGATTCATTC TTTAAATGAT 
AAGAATGCTG CTTTTCTTCT GATGGAAAGT GACAGGCTAA TCGTCAGTTT ACCCAGAGTG 
AAGTGGACAG AGTCAGCATT GATCATGGCA TCTCAGCTCC AAGAAGAATG CATTGCATTT 
ATTATAGAAA ATTTCTCCAA GATCATTCAA AGTGAAAATT TTGCTCTTCT GTTACAGTCA 
CAAGCAATGA GTAGCACATC TGATCTGTTG GACAAAATTT TTAAAGCTGT TGAAGAAAAC 
ATTACCACTG AGAATAGTTG CTCGCTTCTT ATGGCTCTGG ACACATTACT GAACTCTGAC 
AGTACAAAAG AAATGGGTTT TACGTGCAAG ATCCAGGCTC TGCGTGATAA GCTGTGGATC 
TTCCTGGTTC AGTCTTTCTA TGCTGTTCGT CACACAGAAA GCTGGAAGCT GATGAGCACA 
GATGATCAAC AGAAAATCCA AGCAGCTGCA TTTGACAAAG GTGATGACCG AAGACTTGGC 
AAAAAGCCTA TATTCAGTAG CTCTCAGCAA AGAAAACAGG TTTCTGACTG TGGTGCTATA 
AAAAACAAAT CCGCTGGAGG AAGTAACAAG AAACAGTGTC TGAGTTATCT CTCTGCTCAT 
CAGAAGATGA AATCTGATGG ATTAGGAGCC TCTGGACATT CATCAAGTAC TAATAGAAAT 
TCTATAAATA AAACTTTGAA GCATGATGAT TTAAAGGAAA AGGATCCAAA ACCAGCATCC 
AAGAGTACAA AAGAATTAAA AACTGTGGAA AAAAATGTTT CTGGAAAGCC CAAAGCTATC 
ATAAAGCCCA AAACAGAAAA TAGTGATAAT GCAAAGTCAA CAAACCTGTC ACCTAGACAA 
GCTGTGGAAA GATCAGCAAC AGCAGCAGCA AATGAACAGA AAAATTCAGG AAATGGAAAA 
GGAGTGAGAA ATCAGGAAGA GCAAATTACA GGTGCCAGAC CCAAGGTACT CACTGGAAAT 
TTAAATGTGC AAGCCAAAGC AAAGCCTTTG AAGAAAGTGA CAGGCAAAGA TTCTCCACAC 
CTCCGAATGG CAGGGCCCTC CAGCAGATCT ACAAATTCAA GTATGGAATT ACTGACTTCC 
ACTGACTCTC TAGATGAACC AAAAGAAAAT GGGTCAATGA AAGAGAAGCT TCCTGGTCAT 
AAACCTTCCT CTTGTGACTC TCGAGGACAG ACTGTGAAAA ACAGTGTAGA AGGTATCAAA 
ACTTCCACTG TAGCAATGAA ATATCGACCT GTTTCAAAAG ATACCAATGG AATTTCCAAT 
AAAAAAAGCA CTCATGAACA GGAATCTAAT ACACATAACA GTGTGCTAAA GAAGATCACC 
AGCAAAGGAT ACAGTGATCC AGTACCACAG GCAATTTTAA AAAAAAGGGG AAATGGCAAT 
GGATGTGCTA CAGCTCCGCA GAGGACAAAG AATGCCACAG CTAATCTTGC TAAAACTCAA 
GGATCCCAAG GATCACCAAA TTCAGTAAAA TCTTCAGTCT CTTCAAGGCA ATCTGATGAA 
AATGTGACAA AATTGGACCA CAGTGTAACT ACAGATAAAC AGACGCCTAA GAGAAAAATT 
GTCAAGCAAG GACAAACAAC TTTGCCTAAG ATTGGTGCAA AAAAGGTCAC AATGCCTAAA 
AACCCAAATC AACCCAAAAG AGGTGAAAAT TTGAATAATA AAGATGCAAA ACCGAAAACA 
CTTCCTGAAC AGGTTATACT GAAGACTCTG CCTTCTTCTC AGAGACCTTT AAAAAGTGAA 
CCATCTGTTG TCCAAAAAAG TGTGTCTCTT GATGTGTGTG ACAATAATAA CAAAGGCAGT 
GTTTCTGAAC AGAAGCCTCA TGAACCTCTA ACTAATCTCA CAGCCAAAAC CAGTGATGCA 
GAAGCATTTC AGTCACCATG CATACTCGAC TCACAGAAGA CACTGAACAA TCAAGAAAAA 
CAGAAGTTGG TGTTAGAATG CCAAAATATT TCAAATCTGG ATAAATTAAT AAAACGTGAA 
CTGGAATCAA AACAGATCTG TTCAGTTAAA AGTGAAACAA ATGTTTCTGG CCACAAAAAA 
ATAGATCACT GCACCACAGC TAAAATACCT TGTCATTCTG ATGAGACTGA TAATGTCGAT 
CCAAAATTTT ATAGTACCAC TGCTCTAAAA TCCATGATTT CAAATCCTAA AGAAAACTCT 
TTGAACTCTA ATCCAGTTTG TGACTTAGAC TCAGCACACG TAGAACAACT CCATTCAGAA 
TTAGATAGGA AGAAGCAAGT AGGGAGAAAA GATACAAACC AAAAATTAAG CATTAAATGT 
GTGAAAGATG TTTTACCTTG GGTTCCTGAA AAGACAAATG GTACCTTAAA TTCTGGTCAA 
GATGACAGAA AATCTAAAAT TCATGTAGAA GAACAGACAG TTCCTAGTCA GTTGTCTGAT 
GACTCTGCCA TGAATGAAGA CAATCATGCT ACAGTAGAGT CACATATTTC CTCCAAGTGT 
TTTTTGGAAC AAATATCAGG GAAAAATTCT AAAGATACGG AAACAACAGA AACTTCAGAG 
AGCCACGAAA CTCCAGAAGC ACCATTCATG AGTCACTGGA ATTTGAGTAC CAATGTTCTG 
CATCAGAGAG AGAGTCCTGA ATCAGACAGT GGCAGTGTCA CAACATCCTC TGATGACATA 
AAGCCCAGAT CTGAAGACTA TGATGCTGGA GGGTCTCAGG ATGATGATGG GTCAAATGAC 
AGAGGGGTTT CTAAATGTGG CACTATGCTG TGCCATGATT TTCTTGGAAG GAGCAGCAGT 
GATACCAGTA CTCCTGAAGA ATTAAAAATA TATGATAGCA ACTTAAGAAT TGAAGTGAAA 
ATGAAAAAGC AAGGTAGTAG TGATCTTTTC CAAGTTAATT CAACAAGTGA TGATGAGATT 
CCCAGGAAAA GGCCAGAAAT TTGGTCTCGA TCTACAATAG TTCATCCTAG GGAAAAAGAA 
AATATTCCAC GAGGCAGTGT CCAGTTTGTA CAGGAGGTAG ATCAGGTTTC TTCCTCAGCA 
GATGAAACAG AAGATGAAAG GTCTGAAGCT GAAAATGTTG CAGAAAATTT CTCTACATCT 
AACCCAGCTC TTCAGCAGTT TCAGGGGATA ATTAATCTAG CTTTTGAAGA TGCAACTGAA 
AATGAAAGTC ATGAGTTTCG TGTAACTAAA AGTTTTAAAA GGTCTGTTTT ACTTTCCGTA 
GATGAATGTG AAGAGATAGG ATCTGATGAA GGGGAGGTCC ATGCTCTCTT TCAACCTTCT 
GTAGATTCTC TCTCACCTTC TGATGTTTTT GATGGCATTT CTCATGAACA TCATGGAAGG 
ACCTGCTACT CCAGATACTC ACATGGAAGT GAAGGTGGTG TTTTAGAATG CAGACAAAAG 
AAAAGCAATA CTATATATAA AAACAAAAGC TTTCCCTTGG GTCTTAGTAG CGTTGACTCA 
TCAAGAAAAG ATAAACAGAG TGCTTCAGCC ACAGAAAAAA AGGGTACAAC AGATGTCCTG 
TCCAAAGGAG GCATACAGCT TCCTCCAGGA GATAAAGTAT GTAGTGGAAG CAATGTGGAT 
AATGACTTTG AGACACACAG CAAATTTTCA GATAGTGATA TAAAATCTCA AGAAAGACCA 
TGTCATTTGG AACTTCGTCA AAGAGAATCC AATTCCGACA TACCAAAGAA CAGCTTCACA 
AAGTCTCTGG ACTCCTGTCG GAGTCAAGTT CTGCCTCAGG AAGGTCAAGT GAAAGAAAGC 
CATTCTACAG CTACCGAAAA AGCTAATATT GCTTTATCTG CAGGAGACAT AGATGGTTGT 
GACACAGTGG CACAAATCTA CATGTATGAC CATCGGCCTT CAAAAACCCT GTCTCCAATA 
TATGAGATGG ATGTAACAGA AGCATTTGAG CAGAAAATGG AATCAGAAAC ACAAGGTACA 
GACATGGATT TTGAAGATGA GCAACACTTT GCAAAACAAG ACTGGACACT ATTAAAGCAA 
CTGCTCTCTG AACAGGACTC TAACTTAAAT ATCACAAATT CTGTTCCTGA AGACTTAAAT 
TTAGCACAGT ATCTAATTAA TCAGACACTA CTTTTAGCAC GAGACAGCTC AAAACCTCAG 
GGTAAAGCAC ATGTAGACAC TTTGAGCAGA TGGAGTGAAC TAACATCTCC ATTTGATGAT 
TCCTCAGCAA GCATTACCAT GGCTAGTTTT TCCTCTGAAG AATGTTCACC CCAAGGGGAA 
TGGACAATTC TGGAACTGGA AACTCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTBD8 ( XM_010803309.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801611.1 Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.

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CloneID	OBa50260
Clone ID Related Accession (Same CDS sequence)	XM_010803309.3 , XM_010803309.2
Accession Version	XM_010803309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	BTB/POZ domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTA TGATGCACTT TATATACGGA GGAACTTTGG ACTTTCCAGT CAAAGCTAAT 
GTTGGTCAGA TACTCAATAT GGCTGATATG TATGGACTAG AAGGATTAAA AGAAGTAGCA 
ATCTATATTT TAAGAAGAGA TTACTGTAAT TTCTTTCAGA AGCCTGTTTC CAGAAGGTTG 
GCATCTATAT TAGAATGCCT GATTATTGCT CATTCAGTTG GAGTGGAAAG TCTTTTTGCT 
GACTGCATGA AGTGGATTGT AAAGCACTTT GCAAGGTTTT GGTCTGAAAG AAGCTTTGCA 
AATGTACCTC CTGAGATTCA GAAAAGTTGT TTTAATATGT TGATTCATTC TTTAAATGAT 
AAGAATGCTG CTTTTCTTCT GATGGAAAGT GACAGGCTAA TCGTCAGTTT ACCCAGAGTG 
AAGTGGACAG AGTCAGCATT GATCATGGCA TCTCAGCTCC AAGAAGAATG CATTGCATTT 
ATTATAGAAA ATTTCTCCAA GATCATTCAA AGTGAAAATT TTGCTCTTCT GTTACAGTCA 
CAAGCAATGA GTAGCACATC TGATCTGTTG GACAAAATTT TTAAAGCTGT TGAAGAAAAC 
ATTACCACTG AGAATAGTTG CTCGCTTCTT ATGGCTCTGG ACACATTACT GAACTCTGAC 
AGTACAAAAG AAATGGGTTT TACGTGCAAG ATCCAGGCTC TGCGTGATAA GCTGTGGATC 
TTCCTGGTTC AGTCTTTCTA TGCTGTTCGT CACACAGAAA GCTGGAAGCT GATGAGCACA 
GATGATCAAC AGAAAATCCA AGCAGCTGCA TTTGACAAAG GTGATGACCG AAGACTTGGC 
AAAAAGCCTA TATTCAGTAG CTCTCAGCAA AGAAAACAGG TTTCTGACTG TGGTGCTATA 
AAAAACAAAT CCGCTGGAGG AAGTAACAAG AAACAGTGTC TGAGTTATCT CTCTGCTCAT 
CAGAAGATGA AATCTGATGG ATTAGGAGCC TCTGGACATT CATCAAGTAC TAATAGAAAT 
TCTATAAATA AAACTTTGAA GCATGATGAT TTAAAGGAAA AGGATCCAAA ACCAGCATCC 
AAGAGTACAA AAGAATTAAA AACTGTGGAA AAAAATGTTT CTGGAAAGCC CAAAGCTATC 
ATAAAGCCCA AAACAGAAAA TAGTGATAAT GCAAAGTCAA CAAACCTGTC ACCTAGACAA 
GCTGTGGAAA GATCAGCAAC AGCAGCAGCA AATGAACAGA AAAATTCAGG AAATGGAAAA 
GGAGTGAGAA ATCAGGAAGA GCAAATTACA GGTGCCAGAC CCAAGGTACT CACTGGAAAT 
TTAAATGTGC AAGCCAAAGC AAAGCCTTTG AAGAAAGTGA CAGGCAAAGA TTCTCCACAC 
CTCCGAATGG CAGGGCCCTC CAGCAGATCT ACAAATTCAA GTATGGAATT ACTGACTTCC 
ACTGACTCTC TAGATGAACC AAAAGAAAAT GGGTCAATGA AAGAGAAGCT TCCTGGTCAT 
AAACCTTCCT CTTGTGACTC TCGAGGACAG ACTGTGAAAA ACAGTGTAGA AGGTATCAAA 
ACTTCCACTG TAGCAATGAA ATATCGACCT GTTTCAAAAG ATACCAATGG AATTTCCAAT 
AAAAAAAGCA CTCATGAACA GGAATCTAAT ACACATAACA GTGTGCTAAA GAAGATCACC 
AGCAAAGGAT ACAGTGATCC AGTACCACAG GCAATTTTAA AAAAAAGGGG AAATGGCAAT 
GGATGTGCTA CAGCTCCGCA GAGGACAAAG AATGCCACAG CTAATCTTGC TAAAACTCAA 
GGATCCCAAG GATCACCAAA TTCAGTAAAA TCTTCAGTCT CTTCAAGGCA ATCTGATGAA 
AATGTGACAA AATTGGACCA CAGTGTAACT ACAGATAAAC AGACGCCTAA GAGAAAAATT 
GTCAAGCAAG GACAAACAAC TTTGCCTAAG ATTGGTGCAA AAAAGGTCAC AATGCCTAAA 
AACCCAAATC AACCCAAAAG AGGTGAAAAT TTGAATAATA AAGATGCAAA ACCGAAAACA 
CTTCCTGAAC AGGTTATACT GAAGACTCTG CCTTCTTCTC AGAGACCTTT AAAAAGTGAA 
CCATCTGTTG TCCAAAAAAG TGTGTCTCTT GATGTGTGTG ACAATAATAA CAAAGGCAGT 
GTTTCTGAAC AGAAGCCTCA TGAACCTCTA ACTAATCTCA CAGCCAAAAC CAGTGATGCA 
GAAGCATTTC AGTCACCATG CATACTCGAC TCACAGAAGA CACTGAACAA TCAAGAAAAA 
CAGAAGTTGG TGTTAGAATG CCAAAATATT TCAAATCTGG ATAAATTAAT AAAACGTGAA 
CTGGAATCAA AACAGATCTG TTCAGTTAAA AGTGAAACAA ATGTTTCTGG CCACAAAAAA 
ATAGATCACT GCACCACAGC TAAAATACCT TGTCATTCTG ATGAGACTGA TAATGTCGAT 
CCAAAATTTT ATAGTACCAC TGCTCTAAAA TCCATGATTT CAAATCCTAA AGAAAACTCT 
TTGAACTCTA ATCCAGTTTG TGACTTAGAC TCAGCACACG TAGAACAACT CCATTCAGAA 
TTAGATAGGA AGAAGCAAGT AGGGAGAAAA GATACAAACC AAAAATTAAG CATTAAATGT 
GTGAAAGATG TTTTACCTTG GGTTCCTGAA AAGACAAATG GTACCTTAAA TTCTGGTCAA 
GATGACAGAA AATCTAAAAT TCATGTAGAA GAACAGACAG TTCCTAGTCA GTTGTCTGAT 
GACTCTGCCA TGAATGAAGA CAATCATGCT ACAGTAGAGT CACATATTTC CTCCAAGTGT 
TTTTTGGAAC AAATATCAGG GAAAAATTCT AAAGATACGG AAACAACAGA AACTTCAGAG 
AGCCACGAAA CTCCAGAAGC ACCATTCATG AGTCACTGGA ATTTGAGTAC CAATGTTCTG 
CATCAGAGAG AGAGTCCTGA ATCAGACAGT GGCAGTGTCA CAACATCCTC TGATGACATA 
AAGCCCAGAT CTGAAGACTA TGATGCTGGA GGGTCTCAGG ATGATGATGG GTCAAATGAC 
AGAGGGGTTT CTAAATGTGG CACTATGCTG TGCCATGATT TTCTTGGAAG GAGCAGCAGT 
GATACCAGTA CTCCTGAAGA ATTAAAAATA TATGATAGCA ACTTAAGAAT TGAAGTGAAA 
ATGAAAAAGC AAGGTAGTAG TGATCTTTTC CAAGTTAATT CAACAAGTGA TGATGAGATT 
CCCAGGAAAA GGCCAGAAAT TTGGTCTCGA TCTACAATAG TTCATCCTAG GGAAAAAGAA 
AATATTCCAC GAGGCAGTGT CCAGTTTGTA CAGGAGGTAG ATCAGGTTTC TTCCTCAGCA 
GATGAAACAG AAGATGAAAG GTCTGAAGCT GAAAATGTTG CAGAAAATTT CTCTACATCT 
AACCCAGCTC TTCAGCAGTT TCAGGGGATA ATTAATCTAG CTTTTGAAGA TGCAACTGAA 
AATGAAAGTC ATGAGTTTCG TGTAACTAAA AGTTTTAAAA GGTCTGTTTT ACTTTCCGTA 
GATGAATGTG AAGAGATAGG ATCTGATGAA GGGGAGGTCC ATGCTCTCTT TCAACCTTCT 
GTAGATTCTC TCTCACCTTC TGATGTTTTT GATGGCATTT CTCATGAACA TCATGGAAGG 
ACCTGCTACT CCAGATACTC ACATGGAAGT GAAGGTGGTG TTTTAGAATG CAGACAAAAG 
AAAAGCAATA CTATATATAA AAACAAAAGC TTTCCCTTGG GTCTTAGTAG CGTTGACTCA 
TCAAGAAAAG ATAAACAGAG TGCTTCAGCC ACAGAAAAAA AGGGTACAAC AGATGTCCTG 
TCCAAAGGAG GCATACAGCT TCCTCCAGGA GATAAAGTAT GTAGTGGAAG CAATGTGGAT 
AATGACTTTG AGACACACAG CAAATTTTCA GATAGTGATA TAAAATCTCA AGAAAGACCA 
TGTCATTTGG AACTTCGTCA AAGAGAATCC AATTCCGACA TACCAAAGAA CAGCTTCACA 
AAGTCTCTGG ACTCCTGTCG GAGTCAAGTT CTGCCTCAGG AAGGTCAAGT GAAAGAAAGC 
CATTCTACAG CTACCGAAAA AGCTAATATT GCTTTATCTG CAGGAGACAT AGATGGTTGT 
GACACAGTGG CACAAATCTA CATGTATGAC CATCGGCCTT CAAAAACCCT GTCTCCAATA 
TATGAGATGG ATGTAACAGA AGCATTTGAG CAGAAAATGG AATCAGAAAC ACAAGGTACA 
GACATGGATT TTGAAGATGA GCAACACTTT GCAAAACAAG ACTGGACACT ATTAAAGCAA 
CTGCTCTCTG AACAGGACTC TAACTTAAAT ATCACAAATT CTGTTCCTGA AGACTTAAAT 
TTAGCACAGT ATCTAATTAA TCAGACACTA CTTTTAGCAC GAGACAGCTC AAAACCTCAG 
GGTAAAGCAC ATGTAGACAC TTTGAGCAGA TGGAGTGAAC TAACATCTCC ATTTGATGAT 
TCCTCAGCAA GCATTACCAT GGCTAGTTTT TCCTCTGAAG AATGTTCACC CCAAGGGGAA 
TGGACAATTC TGGAACTGGA AACTCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801611.1
CDS	95..4687
Translation	MNVMMHFIYGGTLDFPVKANVGQILNMADMYGLEGLKEVAIYILRRDYCNFFQKPVSRRLASILECLIIAHSVGVESLFADCMKWIVKHFARFWSERSFANVPPEIQKSCFNMLIHSLNDKNAAFLLMESDRLIVSLPRVKWTESALIMASQLQEECIAFIIENFSKIIQSENFALLLQSQAMSSTSDLLDKIFKAVEENITTENSCSLLMALDTLLNSDSTKEMGFTCKIQALRDKLWIFLVQSFYAVRHTESWKLMSTDDQQKIQAAAFDKGDDRRLGKKPIFSSSQQRKQVSDCGAIKNKSAGGSNKKQCLSYLSAHQKMKSDGLGASGHSSSTNRNSINKTLKHDDLKEKDPKPASKSTKELKTVEKNVSGKPKAIIKPKTENSDNAKSTNLSPRQAVERSATAAANEQKNSGNGKGVRNQEEQITGARPKVLTGNLNVQAKAKPLKKVTGKDSPHLRMAGPSSRSTNSSMELLTSTDSLDEPKENGSMKEKLPGHKPSSCDSRGQTVKNSVEGIKTSTVAMKYRPVSKDTNGISNKKSTHEQESNTHNSVLKKITSKGYSDPVPQAILKKRGNGNGCATAPQRTKNATANLAKTQGSQGSPNSVKSSVSSRQSDENVTKLDHSVTTDKQTPKRKIVKQGQTTLPKIGAKKVTMPKNPNQPKRGENLNNKDAKPKTLPEQVILKTLPSSQRPLKSEPSVVQKSVSLDVCDNNNKGSVSEQKPHEPLTNLTAKTSDAEAFQSPCILDSQKTLNNQEKQKLVLECQNISNLDKLIKRELESKQICSVKSETNVSGHKKIDHCTTAKIPCHSDETDNVDPKFYSTTALKSMISNPKENSLNSNPVCDLDSAHVEQLHSELDRKKQVGRKDTNQKLSIKCVKDVLPWVPEKTNGTLNSGQDDRKSKIHVEEQTVPSQLSDDSAMNEDNHATVESHISSKCFLEQISGKNSKDTETTETSESHETPEAPFMSHWNLSTNVLHQRESPESDSGSVTTSSDDIKPRSEDYDAGGSQDDDGSNDRGVSKCGTMLCHDFLGRSSSDTSTPEELKIYDSNLRIEVKMKKQGSSDLFQVNSTSDDEIPRKRPEIWSRSTIVHPREKENIPRGSVQFVQEVDQVSSSADETEDERSEAENVAENFSTSNPALQQFQGIINLAFEDATENESHEFRVTKSFKRSVLLSVDECEEIGSDEGEVHALFQPSVDSLSPSDVFDGISHEHHGRTCYSRYSHGSEGGVLECRQKKSNTIYKNKSFPLGLSSVDSSRKDKQSASATEKKGTTDVLSKGGIQLPPGDKVCSGSNVDNDFETHSKFSDSDIKSQERPCHLELRQRESNSDIPKNSFTKSLDSCRSQVLPQEGQVKESHSTATEKANIALSAGDIDGCDTVAQIYMYDHRPSKTLSPIYEMDVTEAFEQKMESETQGTDMDFEDEQHFAKQDWTLLKQLLSEQDSNLNITNSVPEDLNLAQYLINQTLLLARDSSKPQGKAHVDTLSRWSELTSPFDDSSASITMASFSSEECSPQGEWTILELETQH

Target ORF information:

RefSeq Version	XM_010803309.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BTB (POZ) domain containing 8 (BTBD8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803309.2

ORF Insert Sequence:

ATGAATGTTA TGATGCACTT TATATACGGA GGAACTTTGG ACTTTCCAGT CAAAGCTAAT 
GTTGGTCAGA TACTCAATAT GGCTGATATG TATGGACTAG AAGGATTAAA AGAAGTAGCA 
ATCTATATTT TAAGAAGAGA TTACTGTAAT TTCTTTCAGA AGCCTGTTTC CAGAAGGTTG 
GCATCTATAT TAGAATGCCT GATTATTGCT CATTCAGTTG GAGTGGAAAG TCTTTTTGCT 
GACTGCATGA AGTGGATTGT AAAGCACTTT GCAAGGTTTT GGTCTGAAAG AAGCTTTGCA 
AATGTACCTC CTGAGATTCA GAAAAGTTGT TTTAATATGT TGATTCATTC TTTAAATGAT 
AAGAATGCTG CTTTTCTTCT GATGGAAAGT GACAGGCTAA TCGTCAGTTT ACCCAGAGTG 
AAGTGGACAG AGTCAGCATT GATCATGGCA TCTCAGCTCC AAGAAGAATG CATTGCATTT 
ATTATAGAAA ATTTCTCCAA GATCATTCAA AGTGAAAATT TTGCTCTTCT GTTACAGTCA 
CAAGCAATGA GTAGCACATC TGATCTGTTG GACAAAATTT TTAAAGCTGT TGAAGAAAAC 
ATTACCACTG AGAATAGTTG CTCGCTTCTT ATGGCTCTGG ACACATTACT GAACTCTGAC 
AGTACAAAAG AAATGGGTTT TACGTGCAAG ATCCAGGCTC TGCGTGATAA GCTGTGGATC 
TTCCTGGTTC AGTCTTTCTA TGCTGTTCGT CACACAGAAA GCTGGAAGCT GATGAGCACA 
GATGATCAAC AGAAAATCCA AGCAGCTGCA TTTGACAAAG GTGATGACCG AAGACTTGGC 
AAAAAGCCTA TATTCAGTAG CTCTCAGCAA AGAAAACAGG TTTCTGACTG TGGTGCTATA 
AAAAACAAAT CCGCTGGAGG AAGTAACAAG AAACAGTGTC TGAGTTATCT CTCTGCTCAT 
CAGAAGATGA AATCTGATGG ATTAGGAGCC TCTGGACATT CATCAAGTAC TAATAGAAAT 
TCTATAAATA AAACTTTGAA GCATGATGAT TTAAAGGAAA AGGATCCAAA ACCAGCATCC 
AAGAGTACAA AAGAATTAAA AACTGTGGAA AAAAATGTTT CTGGAAAGCC CAAAGCTATC 
ATAAAGCCCA AAACAGAAAA TAGTGATAAT GCAAAGTCAA CAAACCTGTC ACCTAGACAA 
GCTGTGGAAA GATCAGCAAC AGCAGCAGCA AATGAACAGA AAAATTCAGG AAATGGAAAA 
GGAGTGAGAA ATCAGGAAGA GCAAATTACA GGTGCCAGAC CCAAGGTACT CACTGGAAAT 
TTAAATGTGC AAGCCAAAGC AAAGCCTTTG AAGAAAGTGA CAGGCAAAGA TTCTCCACAC 
CTCCGAATGG CAGGGCCCTC CAGCAGATCT ACAAATTCAA GTATGGAATT ACTGACTTCC 
ACTGACTCTC TAGATGAACC AAAAGAAAAT GGGTCAATGA AAGAGAAGCT TCCTGGTCAT 
AAACCTTCCT CTTGTGACTC TCGAGGACAG ACTGTGAAAA ACAGTGTAGA AGGTATCAAA 
ACTTCCACTG TAGCAATGAA ATATCGACCT GTTTCAAAAG ATACCAATGG AATTTCCAAT 
AAAAAAAGCA CTCATGAACA GGAATCTAAT ACACATAACA GTGTGCTAAA GAAGATCACC 
AGCAAAGGAT ACAGTGATCC AGTACCACAG GCAATTTTAA AAAAAAGGGG AAATGGCAAT 
GGATGTGCTA CAGCTCCGCA GAGGACAAAG AATGCCACAG CTAATCTTGC TAAAACTCAA 
GGATCCCAAG GATCACCAAA TTCAGTAAAA TCTTCAGTCT CTTCAAGGCA ATCTGATGAA 
AATGTGACAA AATTGGACCA CAGTGTAACT ACAGATAAAC AGACGCCTAA GAGAAAAATT 
GTCAAGCAAG GACAAACAAC TTTGCCTAAG ATTGGTGCAA AAAAGGTCAC AATGCCTAAA 
AACCCAAATC AACCCAAAAG AGGTGAAAAT TTGAATAATA AAGATGCAAA ACCGAAAACA 
CTTCCTGAAC AGGTTATACT GAAGACTCTG CCTTCTTCTC AGAGACCTTT AAAAAGTGAA 
CCATCTGTTG TCCAAAAAAG TGTGTCTCTT GATGTGTGTG ACAATAATAA CAAAGGCAGT 
GTTTCTGAAC AGAAGCCTCA TGAACCTCTA ACTAATCTCA CAGCCAAAAC CAGTGATGCA 
GAAGCATTTC AGTCACCATG CATACTCGAC TCACAGAAGA CACTGAACAA TCAAGAAAAA 
CAGAAGTTGG TGTTAGAATG CCAAAATATT TCAAATCTGG ATAAATTAAT AAAACGTGAA 
CTGGAATCAA AACAGATCTG TTCAGTTAAA AGTGAAACAA ATGTTTCTGG CCACAAAAAA 
ATAGATCACT GCACCACAGC TAAAATACCT TGTCATTCTG ATGAGACTGA TAATGTCGAT 
CCAAAATTTT ATAGTACCAC TGCTCTAAAA TCCATGATTT CAAATCCTAA AGAAAACTCT 
TTGAACTCTA ATCCAGTTTG TGACTTAGAC TCAGCACACG TAGAACAACT CCATTCAGAA 
TTAGATAGGA AGAAGCAAGT AGGGAGAAAA GATACAAACC AAAAATTAAG CATTAAATGT 
GTGAAAGATG TTTTACCTTG GGTTCCTGAA AAGACAAATG GTACCTTAAA TTCTGGTCAA 
GATGACAGAA AATCTAAAAT TCATGTAGAA GAACAGACAG TTCCTAGTCA GTTGTCTGAT 
GACTCTGCCA TGAATGAAGA CAATCATGCT ACAGTAGAGT CACATATTTC CTCCAAGTGT 
TTTTTGGAAC AAATATCAGG GAAAAATTCT AAAGATACGG AAACAACAGA AACTTCAGAG 
AGCCACGAAA CTCCAGAAGC ACCATTCATG AGTCACTGGA ATTTGAGTAC CAATGTTCTG 
CATCAGAGAG AGAGTCCTGA ATCAGACAGT GGCAGTGTCA CAACATCCTC TGATGACATA 
AAGCCCAGAT CTGAAGACTA TGATGCTGGA GGGTCTCAGG ATGATGATGG GTCAAATGAC 
AGAGGGGTTT CTAAATGTGG CACTATGCTG TGCCATGATT TTCTTGGAAG GAGCAGCAGT 
GATACCAGTA CTCCTGAAGA ATTAAAAATA TATGATAGCA ACTTAAGAAT TGAAGTGAAA 
ATGAAAAAGC AAGGTAGTAG TGATCTTTTC CAAGTTAATT CAACAAGTGA TGATGAGATT 
CCCAGGAAAA GGCCAGAAAT TTGGTCTCGA TCTACAATAG TTCATCCTAG GGAAAAAGAA 
AATATTCCAC GAGGCAGTGT CCAGTTTGTA CAGGAGGTAG ATCAGGTTTC TTCCTCAGCA 
GATGAAACAG AAGATGAAAG GTCTGAAGCT GAAAATGTTG CAGAAAATTT CTCTACATCT 
AACCCAGCTC TTCAGCAGTT TCAGGGGATA ATTAATCTAG CTTTTGAAGA TGCAACTGAA 
AATGAAAGTC ATGAGTTTCG TGTAACTAAA AGTTTTAAAA GGTCTGTTTT ACTTTCCGTA 
GATGAATGTG AAGAGATAGG ATCTGATGAA GGGGAGGTCC ATGCTCTCTT TCAACCTTCT 
GTAGATTCTC TCTCACCTTC TGATGTTTTT GATGGCATTT CTCATGAACA TCATGGAAGG 
ACCTGCTACT CCAGATACTC ACATGGAAGT GAAGGTGGTG TTTTAGAATG CAGACAAAAG 
AAAAGCAATA CTATATATAA AAACAAAAGC TTTCCCTTGG GTCTTAGTAG CGTTGACTCA 
TCAAGAAAAG ATAAACAGAG TGCTTCAGCC ACAGAAAAAA AGGGTACAAC AGATGTCCTG 
TCCAAAGGAG GCATACAGCT TCCTCCAGGA GATAAAGTAT GTAGTGGAAG CAATGTGGAT 
AATGACTTTG AGACACACAG CAAATTTTCA GATAGTGATA TAAAATCTCA AGAAAGACCA 
TGTCATTTGG AACTTCGTCA AAGAGAATCC AATTCCGACA TACCAAAGAA CAGCTTCACA 
AAGTCTCTGG ACTCCTGTCG GAGTCAAGTT CTGCCTCAGG AAGGTCAAGT GAAAGAAAGC 
CATTCTACAG CTACCGAAAA AGCTAATATT GCTTTATCTG CAGGAGACAT AGATGGTTGT 
GACACAGTGG CACAAATCTA CATGTATGAC CATCGGCCTT CAAAAACCCT GTCTCCAATA 
TATGAGATGG ATGTAACAGA AGCATTTGAG CAGAAAATGG AATCAGAAAC ACAAGGTACA 
GACATGGATT TTGAAGATGA GCAACACTTT GCAAAACAAG ACTGGACACT ATTAAAGCAA 
CTGCTCTCTG AACAGGACTC TAACTTAAAT ATCACAAATT CTGTTCCTGA AGACTTAAAT 
TTAGCACAGT ATCTAATTAA TCAGACACTA CTTTTAGCAC GAGACAGCTC AAAACCTCAG 
GGTAAAGCAC ATGTAGACAC TTTGAGCAGA TGGAGTGAAC TAACATCTCC ATTTGATGAT 
TCCTCAGCAA GCATTACCAT GGCTAGTTTT TCCTCTGAAG AATGTTCACC CCAAGGGGAA 
TGGACAATTC TGGAACTGGA AACTCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	BTBD8
Entrez Gene ID	520456
Full Name	BTB (POZ) domain containing 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	BTBD8	XP_001098569.1
Bos taurus (cattle)	BTBD8	NP_001098670.1
Mus musculus (house mouse)	Btbd8	NP_001242920.1
Homo sapiens (human)	BTBD8	NP_899065.2
Pan troglodytes (chimpanzee)	BTBD8	XP_524768.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

BTBD8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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