MINK1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024980152.1	XP_024835920.1	misshapen-like kinase 1 isoform X13
XM_024980154.1	XP_024835922.1	misshapen-like kinase 1 isoform X15
XM_024980155.1	XP_024835923.1	misshapen-like kinase 1 isoform X16
XM_024980157.1	XP_024835925.1	misshapen-like kinase 1 isoform X18
XM_024980160.1	XP_024835928.1	misshapen-like kinase 1 isoform X21
NM_001099376.1	NP_001092846.1	misshapen-like kinase 1
XM_024980148.1	XP_024835916.1	misshapen-like kinase 1 isoform X9
XM_024980158.1	XP_024835926.1	misshapen-like kinase 1 isoform X19
XM_024980165.1	XP_024835933.1	misshapen-like kinase 1 isoform X26
XM_024980140.1	XP_024835908.1	misshapen-like kinase 1 isoform X1
XM_024980145.1	XP_024835913.1	misshapen-like kinase 1 isoform X6
XM_024980149.1	XP_024835917.1	misshapen-like kinase 1 isoform X10
XM_024980150.1	XP_024835918.1	misshapen-like kinase 1 isoform X11
XM_024980156.1	XP_024835924.1	misshapen-like kinase 1 isoform X17
XM_024980159.1	XP_024835927.1	misshapen-like kinase 1 isoform X20
XM_024980161.1	XP_024835929.1	misshapen-like kinase 1 isoform X22
XM_024980162.1	XP_024835930.1	misshapen-like kinase 1 isoform X23
XM_024980141.1	XP_024835909.1	misshapen-like kinase 1 isoform X2
XM_024980142.1	XP_024835910.1	misshapen-like kinase 1 isoform X3
XM_024980143.1	XP_024835911.1	misshapen-like kinase 1 isoform X4
XM_024980144.1	XP_024835912.1	misshapen-like kinase 1 isoform X5
XM_024980146.1	XP_024835914.1	misshapen-like kinase 1 isoform X7
XM_024980147.1	XP_024835915.1	misshapen-like kinase 1 isoform X8
XM_024980151.1	XP_024835919.1	misshapen-like kinase 1 isoform X12
XM_024980153.1	XP_024835921.1	misshapen-like kinase 1 isoform X14
XM_024980163.1	XP_024835931.1	misshapen-like kinase 1 isoform X24
XM_024980164.1	XP_024835932.1	misshapen-like kinase 1 isoform X25
XM_024980166.1	XP_024835934.1	misshapen-like kinase 1 isoform X27

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226348	XM_024980140.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226349	XM_024980141.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226350	XM_024980142.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226351	XM_024980143.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226352	XM_024980144.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226353	XM_024980145.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226354	XM_024980146.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226355	XM_024980147.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226356	XM_024980148.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226357	XM_024980149.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226358	XM_024980150.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226359	XM_024980151.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226360	XM_024980152.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226361	XM_024980153.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226362	XM_024980154.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226363	XM_024980155.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226364	XM_024980156.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226365	XM_024980157.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226366	XM_024980158.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226367	XM_024980159.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226368	XM_024980160.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226369	XM_024980161.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226370	XM_024980162.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226371	XM_024980163.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226372	XM_024980164.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226373	XM_024980165.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226374	XM_024980166.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa40630	NM_001099376.1 Latest version!	Bos taurus misshapen like kinase 1 (MINK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

MINK1 ( XM_024980140.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835908.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226348
Clone ID Related Accession (Same CDS sequence)	XM_024980140.1
Accession Version	XM_024980140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCAGCGATG GAGACACAGA CAGCGTCAGC 
ACCATGGTGG TCCACGACGT GGAAGAGATA GCCGGGCCCC AGACCCCCTA CGGGGGTGGC 
ACCATGGTGG TCCAGCGAAC CCCCGAGGAG GAGCGCAGCC TGTTGCATGC CGACAGCAAC 
GGCTACACAA ACCTGCCAGA CGTGGTCCAG CCCAGCCACT CGCCCACTGA GAGCAGCAAA 
GGTCAAAGCC CCCCCTTGAA GGATGGAGGC AGTGACTACC AGTCTCGTGG GCTGGTAAAA 
GCCCCTGGCA AGAGCTCATT CACGATGTTT GTGGACCTGG GGATCTACCA GCCCGGAGGC 
AGTGGGGACA CCATTCCCAT TACAGCTCTG GTGGGTGGAG AGGGCAGCCG GCTCGATCAG 
CTACAGTACG ACGTGAGGAA AGGCTCTGTA GTCAACGTGA ACCCCACCAA CACCCGGGCC 
CACAGCGAAA CCCCCGAGAT TCGGAAGTAC AAGAAGCGGT TCAATTCTGA GATCCTCTGT 
GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG GGCACGGAGA ATGGGCTGAT GTTGCTGGAC 
CGCAGCGGGC AGGGCAAGGT GTATGGACTC ATCGGCCGGC GGCGCTTCCA GCAAATGGAC 
GTGCTGGAGG GACTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTG 
TATTACCTGT CCTGGCTCCG GAACAAGATT CTGCACAATG ACCCGGAAGT GGAGAAGAAG 
CAAGGCTGGA CCACTGTGGG GGACATGGAG GGCTGCGGGC ACTACCGCGT GGTGAAATAC 
GAACGCATCA AGTTCCTAGT CATCGCCCTG AAGAACTCTG TGGAGGTGTA TGCCTGGGCC 
CCCAAACCTT ACCACAAATT CATGGCTTTC AAGTCCTTTG CTGACCTCCC GCACCGCCCT 
CTGCTGGTGG ACCTGACCGT AGAGGAGGGC CAGCGGCTCA AGGTCATCTA TGGCTCCAGC 
GCCGGCTTCC ACGCTGTGGA TGTGGACTCG GGGAACAGCT ACGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC GGACGGCATG 
GAGATGCTGC TGTGCTATGA GGATGAGGGT GTCTACGTCA ACACGTACGG CCGGATCATC 
AAGGACGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATCATGG GCTGGGGAGA GAAAGCCATT GAGATCCGCT CCGTGGAGAC GGGCCACCTG 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGGAACGAC 
AAGGTATTTT TCGCCTCGGT CCGCTCCGGG GGCAGCAGCC AAGTTTACTT CATGACCTTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835908.1
CDS	228..4334
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980140.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980140.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCAGCGATG GAGACACAGA CAGCGTCAGC 
ACCATGGTGG TCCACGACGT GGAAGAGATA GCCGGGCCCC AGACCCCCTA CGGGGGTGGC 
ACCATGGTGG TCCAGCGAAC CCCCGAGGAG GAGCGCAGCC TGTTGCATGC CGACAGCAAC 
GGCTACACAA ACCTGCCAGA CGTGGTCCAG CCCAGCCACT CGCCCACTGA GAGCAGCAAA 
GGTCAAAGCC CCCCCTTGAA GGATGGAGGC AGTGACTACC AGTCTCGTGG GCTGGTAAAA 
GCCCCTGGCA AGAGCTCATT CACGATGTTT GTGGACCTGG GGATCTACCA GCCCGGAGGC 
AGTGGGGACA CCATTCCCAT TACAGCTCTG GTGGGTGGAG AGGGCAGCCG GCTCGATCAG 
CTACAGTACG ACGTGAGGAA AGGCTCTGTA GTCAACGTGA ACCCCACCAA CACCCGGGCC 
CACAGCGAAA CCCCCGAGAT TCGGAAGTAC AAGAAGCGGT TCAATTCTGA GATCCTCTGT 
GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG GGCACGGAGA ATGGGCTGAT GTTGCTGGAC 
CGCAGCGGGC AGGGCAAGGT GTATGGACTC ATCGGCCGGC GGCGCTTCCA GCAAATGGAC 
GTGCTGGAGG GACTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTG 
TATTACCTGT CCTGGCTCCG GAACAAGATT CTGCACAATG ACCCGGAAGT GGAGAAGAAG 
CAAGGCTGGA CCACTGTGGG GGACATGGAG GGCTGCGGGC ACTACCGCGT GGTGAAATAC 
GAACGCATCA AGTTCCTAGT CATCGCCCTG AAGAACTCTG TGGAGGTGTA TGCCTGGGCC 
CCCAAACCTT ACCACAAATT CATGGCTTTC AAGTCCTTTG CTGACCTCCC GCACCGCCCT 
CTGCTGGTGG ACCTGACCGT AGAGGAGGGC CAGCGGCTCA AGGTCATCTA TGGCTCCAGC 
GCCGGCTTCC ACGCTGTGGA TGTGGACTCG GGGAACAGCT ACGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC GGACGGCATG 
GAGATGCTGC TGTGCTATGA GGATGAGGGT GTCTACGTCA ACACGTACGG CCGGATCATC 
AAGGACGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATCATGG GCTGGGGAGA GAAAGCCATT GAGATCCGCT CCGTGGAGAC GGGCCACCTG 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGGAACGAC 
AAGGTATTTT TCGCCTCGGT CCGCTCCGGG GGCAGCAGCC AAGTTTACTT CATGACCTTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980141.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835909.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226349
Clone ID Related Accession (Same CDS sequence)	XM_024980141.1
Accession Version	XM_024980141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCGATGGAG ACACAGACAG CGTCAGCACC 
ATGGTGGTCC ACGACGTGGA AGAGATAGCC GGGCCCCAGA CCCCCTACGG GGGTGGCACC 
ATGGTGGTCC AGCGAACCCC CGAGGAGGAG CGCAGCCTGT TGCATGCCGA CAGCAACGGC 
TACACAAACC TGCCAGACGT GGTCCAGCCC AGCCACTCGC CCACTGAGAG CAGCAAAGGT 
CAAAGCCCCC CCTTGAAGGA TGGAGGCAGT GACTACCAGT CTCGTGGGCT GGTAAAAGCC 
CCTGGCAAGA GCTCATTCAC GATGTTTGTG GACCTGGGGA TCTACCAGCC CGGAGGCAGT 
GGGGACACCA TTCCCATTAC AGCTCTGGTG GGTGGAGAGG GCAGCCGGCT CGATCAGCTA 
CAGTACGACG TGAGGAAAGG CTCTGTAGTC AACGTGAACC CCACCAACAC CCGGGCCCAC 
AGCGAAACCC CCGAGATTCG GAAGTACAAG AAGCGGTTCA ATTCTGAGAT CCTCTGTGCG 
GCCCTTTGGG GGGTCAACCT GCTGGTGGGC ACGGAGAATG GGCTGATGTT GCTGGACCGC 
AGCGGGCAGG GCAAGGTGTA TGGACTCATC GGCCGGCGGC GCTTCCAGCA AATGGACGTG 
CTGGAGGGAC TCAACTTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTGTAT 
TACCTGTCCT GGCTCCGGAA CAAGATTCTG CACAATGACC CGGAAGTGGA GAAGAAGCAA 
GGCTGGACCA CTGTGGGGGA CATGGAGGGC TGCGGGCACT ACCGCGTGGT GAAATACGAA 
CGCATCAAGT TCCTAGTCAT CGCCCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCC 
AAACCTTACC ACAAATTCAT GGCTTTCAAG TCCTTTGCTG ACCTCCCGCA CCGCCCTCTG 
CTGGTGGACC TGACCGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGCGCC 
GGCTTCCACG CTGTGGATGT GGACTCGGGG AACAGCTACG ACATCTACAT CCCTGTACAT 
ATCCAGAGCC AGATCACACC CCACGCCATC ATCTTCCTCC CCAACACGGA CGGCATGGAG 
ATGCTGCTGT GCTATGAGGA TGAGGGTGTC TACGTCAACA CGTACGGCCG GATCATCAAG 
GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTGG CCTACATCTG CTCCAACCAG 
ATCATGGGCT GGGGAGAGAA AGCCATTGAG ATCCGCTCCG TGGAGACGGG CCACCTGGAT 
GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAACGACAAG 
GTATTTTTCG CCTCGGTCCG CTCCGGGGGC AGCAGCCAAG TTTACTTCAT GACCTTGAAC 
CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835909.1
CDS	228..4331
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980141.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980141.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCGATGGAG ACACAGACAG CGTCAGCACC 
ATGGTGGTCC ACGACGTGGA AGAGATAGCC GGGCCCCAGA CCCCCTACGG GGGTGGCACC 
ATGGTGGTCC AGCGAACCCC CGAGGAGGAG CGCAGCCTGT TGCATGCCGA CAGCAACGGC 
TACACAAACC TGCCAGACGT GGTCCAGCCC AGCCACTCGC CCACTGAGAG CAGCAAAGGT 
CAAAGCCCCC CCTTGAAGGA TGGAGGCAGT GACTACCAGT CTCGTGGGCT GGTAAAAGCC 
CCTGGCAAGA GCTCATTCAC GATGTTTGTG GACCTGGGGA TCTACCAGCC CGGAGGCAGT 
GGGGACACCA TTCCCATTAC AGCTCTGGTG GGTGGAGAGG GCAGCCGGCT CGATCAGCTA 
CAGTACGACG TGAGGAAAGG CTCTGTAGTC AACGTGAACC CCACCAACAC CCGGGCCCAC 
AGCGAAACCC CCGAGATTCG GAAGTACAAG AAGCGGTTCA ATTCTGAGAT CCTCTGTGCG 
GCCCTTTGGG GGGTCAACCT GCTGGTGGGC ACGGAGAATG GGCTGATGTT GCTGGACCGC 
AGCGGGCAGG GCAAGGTGTA TGGACTCATC GGCCGGCGGC GCTTCCAGCA AATGGACGTG 
CTGGAGGGAC TCAACTTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTGTAT 
TACCTGTCCT GGCTCCGGAA CAAGATTCTG CACAATGACC CGGAAGTGGA GAAGAAGCAA 
GGCTGGACCA CTGTGGGGGA CATGGAGGGC TGCGGGCACT ACCGCGTGGT GAAATACGAA 
CGCATCAAGT TCCTAGTCAT CGCCCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCC 
AAACCTTACC ACAAATTCAT GGCTTTCAAG TCCTTTGCTG ACCTCCCGCA CCGCCCTCTG 
CTGGTGGACC TGACCGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGCGCC 
GGCTTCCACG CTGTGGATGT GGACTCGGGG AACAGCTACG ACATCTACAT CCCTGTACAT 
ATCCAGAGCC AGATCACACC CCACGCCATC ATCTTCCTCC CCAACACGGA CGGCATGGAG 
ATGCTGCTGT GCTATGAGGA TGAGGGTGTC TACGTCAACA CGTACGGCCG GATCATCAAG 
GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTGG CCTACATCTG CTCCAACCAG 
ATCATGGGCT GGGGAGAGAA AGCCATTGAG ATCCGCTCCG TGGAGACGGG CCACCTGGAT 
GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAACGACAAG 
GTATTTTTCG CCTCGGTCCG CTCCGGGGGC AGCAGCCAAG TTTACTTCAT GACCTTGAAC 
CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980142.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835910.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa226350
Clone ID Related Accession (Same CDS sequence)	XM_024980142.1
Accession Version	XM_024980142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAGATTTC GTCTTGCTGA AAGAGCGGAC CCTGGACGAG 
GCCCCCCGGC CTCCCAAGAA GGCGATGGAC TACTCATCGT CCAGCGAGGA GGTGGAGAGC 
AGCGAGGACG AGGAGGAGGA GAGCAACGGC GAGCCGTCAG AGGGGAGCAG GGACGCCCCT 
GGGGCCCGCA GCGATGGAGA CACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTGGAA 
GAGATAGCCG GGCCCCAGAC CCCCTACGGG GGTGGCACCA TGGTGGTCCA GCGAACCCCC 
GAGGAGGAGC GCAGCCTGTT GCATGCCGAC AGCAACGGCT ACACAAACCT GCCAGACGTG 
GTCCAGCCCA GCCACTCGCC CACTGAGAGC AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT 
GGAGGCAGTG ACTACCAGTC TCGTGGGCTG GTAAAAGCCC CTGGCAAGAG CTCATTCACG 
ATGTTTGTGG ACCTGGGGAT CTACCAGCCC GGAGGCAGTG GGGACACCAT TCCCATTACA 
GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC GATCAGCTAC AGTACGACGT GAGGAAAGGC 
TCTGTAGTCA ACGTGAACCC CACCAACACC CGGGCCCACA GCGAAACCCC CGAGATTCGG 
AAGTACAAGA AGCGGTTCAA TTCTGAGATC CTCTGTGCGG CCCTTTGGGG GGTCAACCTG 
CTGGTGGGCA CGGAGAATGG GCTGATGTTG CTGGACCGCA GCGGGCAGGG CAAGGTGTAT 
GGACTCATCG GCCGGCGGCG CTTCCAGCAA ATGGACGTGC TGGAGGGACT CAACTTGCTC 
ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC 
AAGATTCTGC ACAATGACCC GGAAGTGGAG AAGAAGCAAG GCTGGACCAC TGTGGGGGAC 
ATGGAGGGCT GCGGGCACTA CCGCGTGGTG AAATACGAAC GCATCAAGTT CCTAGTCATC 
GCCCTGAAGA ACTCTGTGGA GGTGTATGCC TGGGCCCCCA AACCTTACCA CAAATTCATG 
GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC CGCCCTCTGC TGGTGGACCT GACCGTAGAG 
GAGGGCCAGC GGCTCAAGGT CATCTATGGC TCCAGCGCCG GCTTCCACGC TGTGGATGTG 
GACTCGGGGA ACAGCTACGA CATCTACATC CCTGTACATA TCCAGAGCCA GATCACACCC 
CACGCCATCA TCTTCCTCCC CAACACGGAC GGCATGGAGA TGCTGCTGTG CTATGAGGAT 
GAGGGTGTCT ACGTCAACAC GTACGGCCGG ATCATCAAGG ACGTGGTGCT GCAGTGGGGA 
GAGATGCCCA CCTCTGTGGC CTACATCTGC TCCAACCAGA TCATGGGCTG GGGAGAGAAA 
GCCATTGAGA TCCGCTCCGT GGAGACGGGC CACCTGGATG GGGTCTTCAT GCACAAACGA 
GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG AACGACAAGG TATTTTTCGC CTCGGTCCGC 
TCCGGGGGCA GCAGCCAAGT TTACTTCATG ACCTTGAACC GTAACTGCAT CATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835910.1
CDS	228..4310
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980142.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980142.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAGATTTC GTCTTGCTGA AAGAGCGGAC CCTGGACGAG 
GCCCCCCGGC CTCCCAAGAA GGCGATGGAC TACTCATCGT CCAGCGAGGA GGTGGAGAGC 
AGCGAGGACG AGGAGGAGGA GAGCAACGGC GAGCCGTCAG AGGGGAGCAG GGACGCCCCT 
GGGGCCCGCA GCGATGGAGA CACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTGGAA 
GAGATAGCCG GGCCCCAGAC CCCCTACGGG GGTGGCACCA TGGTGGTCCA GCGAACCCCC 
GAGGAGGAGC GCAGCCTGTT GCATGCCGAC AGCAACGGCT ACACAAACCT GCCAGACGTG 
GTCCAGCCCA GCCACTCGCC CACTGAGAGC AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT 
GGAGGCAGTG ACTACCAGTC TCGTGGGCTG GTAAAAGCCC CTGGCAAGAG CTCATTCACG 
ATGTTTGTGG ACCTGGGGAT CTACCAGCCC GGAGGCAGTG GGGACACCAT TCCCATTACA 
GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC GATCAGCTAC AGTACGACGT GAGGAAAGGC 
TCTGTAGTCA ACGTGAACCC CACCAACACC CGGGCCCACA GCGAAACCCC CGAGATTCGG 
AAGTACAAGA AGCGGTTCAA TTCTGAGATC CTCTGTGCGG CCCTTTGGGG GGTCAACCTG 
CTGGTGGGCA CGGAGAATGG GCTGATGTTG CTGGACCGCA GCGGGCAGGG CAAGGTGTAT 
GGACTCATCG GCCGGCGGCG CTTCCAGCAA ATGGACGTGC TGGAGGGACT CAACTTGCTC 
ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC 
AAGATTCTGC ACAATGACCC GGAAGTGGAG AAGAAGCAAG GCTGGACCAC TGTGGGGGAC 
ATGGAGGGCT GCGGGCACTA CCGCGTGGTG AAATACGAAC GCATCAAGTT CCTAGTCATC 
GCCCTGAAGA ACTCTGTGGA GGTGTATGCC TGGGCCCCCA AACCTTACCA CAAATTCATG 
GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC CGCCCTCTGC TGGTGGACCT GACCGTAGAG 
GAGGGCCAGC GGCTCAAGGT CATCTATGGC TCCAGCGCCG GCTTCCACGC TGTGGATGTG 
GACTCGGGGA ACAGCTACGA CATCTACATC CCTGTACATA TCCAGAGCCA GATCACACCC 
CACGCCATCA TCTTCCTCCC CAACACGGAC GGCATGGAGA TGCTGCTGTG CTATGAGGAT 
GAGGGTGTCT ACGTCAACAC GTACGGCCGG ATCATCAAGG ACGTGGTGCT GCAGTGGGGA 
GAGATGCCCA CCTCTGTGGC CTACATCTGC TCCAACCAGA TCATGGGCTG GGGAGAGAAA 
GCCATTGAGA TCCGCTCCGT GGAGACGGGC CACCTGGATG GGGTCTTCAT GCACAAACGA 
GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG AACGACAAGG TATTTTTCGC CTCGGTCCGC 
TCCGGGGGCA GCAGCCAAGT TTACTTCATG ACCTTGAACC GTAACTGCAT CATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980143.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835911.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X4, mRNA.

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CloneID	OBa226351
Clone ID Related Accession (Same CDS sequence)	XM_024980143.1
Accession Version	XM_024980143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAGATTTC GTCTTGCTGA AAGAGCGGAC CCTGGACGAG 
GCCCCCCGGC CTCCCAAGAA GGCGATGGAC TACTCATCGT CCAGCGAGGA GGTGGAGAGC 
AGCGAGGACG AGGAGGAGGA GAGCAACGGC GAGCCGTCAG AGGGGAGCAG GGACGCCCCT 
GGGGCCCGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835911.1
CDS	228..4307
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980143.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980143.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAGATTTC GTCTTGCTGA AAGAGCGGAC CCTGGACGAG 
GCCCCCCGGC CTCCCAAGAA GGCGATGGAC TACTCATCGT CCAGCGAGGA GGTGGAGAGC 
AGCGAGGACG AGGAGGAGGA GAGCAACGGC GAGCCGTCAG AGGGGAGCAG GGACGCCCCT 
GGGGCCCGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980144.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835912.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa226352
Clone ID Related Accession (Same CDS sequence)	XM_024980144.1
Accession Version	XM_024980144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCAGCGATG GAGACACAGA CAGCGTCAGC 
ACCATGGTGG TCCACGACGT GGAAGAGATA GCCGGGCCCC AGACCCCCTA CGGGGGTGGC 
ACCATGGTGG TCCAGCGAAC CCCCGAGGAG GAGCGCAGCC TGTTGCATGC CGACAGCAAC 
GGCTACACAA ACCTGCCAGA CGTGGTCCAG CCCAGCCACT CGCCCACTGA GAGCAGCAAA 
GGTCAAAGCC CCCCCTTGAA GGATGGAGGC AGTGACTACC AGTCTCGTGG GCTGGTAAAA 
GCCCCTGGCA AGAGCTCATT CACGATGTTT GTGGACCTGG GGATCTACCA GCCCGGAGGC 
AGTGGGGACA CCATTCCCAT TACAGCTCTG GTGGGTGGAG AGGGCAGCCG GCTCGATCAG 
CTACAGTACG ACGTGAGGAA AGGCTCTGTA GTCAACGTGA ACCCCACCAA CACCCGGGCC 
CACAGCGAAA CCCCCGAGAT TCGGAAGTAC AAGAAGCGGT TCAATTCTGA GATCCTCTGT 
GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG GGCACGGAGA ATGGGCTGAT GTTGCTGGAC 
CGCAGCGGGC AGGGCAAGGT GTATGGACTC ATCGGCCGGC GGCGCTTCCA GCAAATGGAC 
GTGCTGGAGG GACTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTG 
TATTACCTGT CCTGGCTCCG GAACAAGATT CTGCACAATG ACCCGGAAGT GGAGAAGAAG 
CAAGGCTGGA CCACTGTGGG GGACATGGAG GGCTGCGGGC ACTACCGCGT GGTGAAATAC 
GAACGCATCA AGTTCCTAGT CATCGCCCTG AAGAACTCTG TGGAGGTGTA TGCCTGGGCC 
CCCAAACCTT ACCACAAATT CATGGCTTTC AAGTCCTTTG CTGACCTCCC GCACCGCCCT 
CTGCTGGTGG ACCTGACCGT AGAGGAGGGC CAGCGGCTCA AGGTCATCTA TGGCTCCAGC 
GCCGGCTTCC ACGCTGTGGA TGTGGACTCG GGGAACAGCT ACGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC GGACGGCATG 
GAGATGCTGC TGTGCTATGA GGATGAGGGT GTCTACGTCA ACACGTACGG CCGGATCATC 
AAGGACGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATCATGG GCTGGGGAGA GAAAGCCATT GAGATCCGCT CCGTGGAGAC GGGCCACCTG 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGGAACGAC 
AAGGTATTTT TCGCCTCGGT CCGCTCCGGG GGCAGCAGCC AAGTTTACTT CATGACCTTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835912.1
CDS	228..4274
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980144.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980144.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCAGCGATG GAGACACAGA CAGCGTCAGC 
ACCATGGTGG TCCACGACGT GGAAGAGATA GCCGGGCCCC AGACCCCCTA CGGGGGTGGC 
ACCATGGTGG TCCAGCGAAC CCCCGAGGAG GAGCGCAGCC TGTTGCATGC CGACAGCAAC 
GGCTACACAA ACCTGCCAGA CGTGGTCCAG CCCAGCCACT CGCCCACTGA GAGCAGCAAA 
GGTCAAAGCC CCCCCTTGAA GGATGGAGGC AGTGACTACC AGTCTCGTGG GCTGGTAAAA 
GCCCCTGGCA AGAGCTCATT CACGATGTTT GTGGACCTGG GGATCTACCA GCCCGGAGGC 
AGTGGGGACA CCATTCCCAT TACAGCTCTG GTGGGTGGAG AGGGCAGCCG GCTCGATCAG 
CTACAGTACG ACGTGAGGAA AGGCTCTGTA GTCAACGTGA ACCCCACCAA CACCCGGGCC 
CACAGCGAAA CCCCCGAGAT TCGGAAGTAC AAGAAGCGGT TCAATTCTGA GATCCTCTGT 
GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG GGCACGGAGA ATGGGCTGAT GTTGCTGGAC 
CGCAGCGGGC AGGGCAAGGT GTATGGACTC ATCGGCCGGC GGCGCTTCCA GCAAATGGAC 
GTGCTGGAGG GACTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTG 
TATTACCTGT CCTGGCTCCG GAACAAGATT CTGCACAATG ACCCGGAAGT GGAGAAGAAG 
CAAGGCTGGA CCACTGTGGG GGACATGGAG GGCTGCGGGC ACTACCGCGT GGTGAAATAC 
GAACGCATCA AGTTCCTAGT CATCGCCCTG AAGAACTCTG TGGAGGTGTA TGCCTGGGCC 
CCCAAACCTT ACCACAAATT CATGGCTTTC AAGTCCTTTG CTGACCTCCC GCACCGCCCT 
CTGCTGGTGG ACCTGACCGT AGAGGAGGGC CAGCGGCTCA AGGTCATCTA TGGCTCCAGC 
GCCGGCTTCC ACGCTGTGGA TGTGGACTCG GGGAACAGCT ACGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC GGACGGCATG 
GAGATGCTGC TGTGCTATGA GGATGAGGGT GTCTACGTCA ACACGTACGG CCGGATCATC 
AAGGACGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATCATGG GCTGGGGAGA GAAAGCCATT GAGATCCGCT CCGTGGAGAC GGGCCACCTG 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGGAACGAC 
AAGGTATTTT TCGCCTCGGT CCGCTCCGGG GGCAGCAGCC AAGTTTACTT CATGACCTTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980145.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835913.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa226353
Clone ID Related Accession (Same CDS sequence)	XM_024980145.1
Accession Version	XM_024980145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCGATGGAG ACACAGACAG CGTCAGCACC 
ATGGTGGTCC ACGACGTGGA AGAGATAGCC GGGCCCCAGA CCCCCTACGG GGGTGGCACC 
ATGGTGGTCC AGCGAACCCC CGAGGAGGAG CGCAGCCTGT TGCATGCCGA CAGCAACGGC 
TACACAAACC TGCCAGACGT GGTCCAGCCC AGCCACTCGC CCACTGAGAG CAGCAAAGGT 
CAAAGCCCCC CCTTGAAGGA TGGAGGCAGT GACTACCAGT CTCGTGGGCT GGTAAAAGCC 
CCTGGCAAGA GCTCATTCAC GATGTTTGTG GACCTGGGGA TCTACCAGCC CGGAGGCAGT 
GGGGACACCA TTCCCATTAC AGCTCTGGTG GGTGGAGAGG GCAGCCGGCT CGATCAGCTA 
CAGTACGACG TGAGGAAAGG CTCTGTAGTC AACGTGAACC CCACCAACAC CCGGGCCCAC 
AGCGAAACCC CCGAGATTCG GAAGTACAAG AAGCGGTTCA ATTCTGAGAT CCTCTGTGCG 
GCCCTTTGGG GGGTCAACCT GCTGGTGGGC ACGGAGAATG GGCTGATGTT GCTGGACCGC 
AGCGGGCAGG GCAAGGTGTA TGGACTCATC GGCCGGCGGC GCTTCCAGCA AATGGACGTG 
CTGGAGGGAC TCAACTTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTGTAT 
TACCTGTCCT GGCTCCGGAA CAAGATTCTG CACAATGACC CGGAAGTGGA GAAGAAGCAA 
GGCTGGACCA CTGTGGGGGA CATGGAGGGC TGCGGGCACT ACCGCGTGGT GAAATACGAA 
CGCATCAAGT TCCTAGTCAT CGCCCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCC 
AAACCTTACC ACAAATTCAT GGCTTTCAAG TCCTTTGCTG ACCTCCCGCA CCGCCCTCTG 
CTGGTGGACC TGACCGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGCGCC 
GGCTTCCACG CTGTGGATGT GGACTCGGGG AACAGCTACG ACATCTACAT CCCTGTACAT 
ATCCAGAGCC AGATCACACC CCACGCCATC ATCTTCCTCC CCAACACGGA CGGCATGGAG 
ATGCTGCTGT GCTATGAGGA TGAGGGTGTC TACGTCAACA CGTACGGCCG GATCATCAAG 
GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTGG CCTACATCTG CTCCAACCAG 
ATCATGGGCT GGGGAGAGAA AGCCATTGAG ATCCGCTCCG TGGAGACGGG CCACCTGGAT 
GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAACGACAAG 
GTATTTTTCG CCTCGGTCCG CTCCGGGGGC AGCAGCCAAG TTTACTTCAT GACCTTGAAC 
CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835913.1
CDS	228..4271
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980145.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980145.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GACCTCGCAG CAACTCCGCC TGGCAAATCT ATCTGCAAAG GCGGGCAGAG 
CGGGGCAGCC CCAAGCCTCC AGGGCCCCCC GCTCCGCCCC CCGGCCCGCC CAACGCCTGT 
AGTAACCCCG ACCTCAGGAG GAGCGACCCC GGCTGGGAGC GCTCAGACAG CGTCCTCCCG 
GCCTCTCATG GGCACCTCCC ACAAGCCGGC TCGCTGGAGC GGAACCGGGT GGGAGCCTCC 
TCCAAACTGG ACAACTCCCC TGTGCTCTCC CCCGGAAACA AAACCAAGCC TGATGACCAC 
CGCTCGCGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCCA TTGGTGAGGA TTTCGTCTTG 
CTGAAAGAGC GGACCCTGGA CGAGGCCCCC CGGCCTCCCA AGAAGGCGAT GGACTACTCA 
TCGTCCAGCG AGGAGGTGGA GAGCAGCGAG GACGAGGAGG AGGAGAGCAA CGGCGAGCCG 
TCAGAGGGGA GCAGGGACGC CCCTGGGGCC CGCGATGGAG ACACAGACAG CGTCAGCACC 
ATGGTGGTCC ACGACGTGGA AGAGATAGCC GGGCCCCAGA CCCCCTACGG GGGTGGCACC 
ATGGTGGTCC AGCGAACCCC CGAGGAGGAG CGCAGCCTGT TGCATGCCGA CAGCAACGGC 
TACACAAACC TGCCAGACGT GGTCCAGCCC AGCCACTCGC CCACTGAGAG CAGCAAAGGT 
CAAAGCCCCC CCTTGAAGGA TGGAGGCAGT GACTACCAGT CTCGTGGGCT GGTAAAAGCC 
CCTGGCAAGA GCTCATTCAC GATGTTTGTG GACCTGGGGA TCTACCAGCC CGGAGGCAGT 
GGGGACACCA TTCCCATTAC AGCTCTGGTG GGTGGAGAGG GCAGCCGGCT CGATCAGCTA 
CAGTACGACG TGAGGAAAGG CTCTGTAGTC AACGTGAACC CCACCAACAC CCGGGCCCAC 
AGCGAAACCC CCGAGATTCG GAAGTACAAG AAGCGGTTCA ATTCTGAGAT CCTCTGTGCG 
GCCCTTTGGG GGGTCAACCT GCTGGTGGGC ACGGAGAATG GGCTGATGTT GCTGGACCGC 
AGCGGGCAGG GCAAGGTGTA TGGACTCATC GGCCGGCGGC GCTTCCAGCA AATGGACGTG 
CTGGAGGGAC TCAACTTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTGTAT 
TACCTGTCCT GGCTCCGGAA CAAGATTCTG CACAATGACC CGGAAGTGGA GAAGAAGCAA 
GGCTGGACCA CTGTGGGGGA CATGGAGGGC TGCGGGCACT ACCGCGTGGT GAAATACGAA 
CGCATCAAGT TCCTAGTCAT CGCCCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCC 
AAACCTTACC ACAAATTCAT GGCTTTCAAG TCCTTTGCTG ACCTCCCGCA CCGCCCTCTG 
CTGGTGGACC TGACCGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGCGCC 
GGCTTCCACG CTGTGGATGT GGACTCGGGG AACAGCTACG ACATCTACAT CCCTGTACAT 
ATCCAGAGCC AGATCACACC CCACGCCATC ATCTTCCTCC CCAACACGGA CGGCATGGAG 
ATGCTGCTGT GCTATGAGGA TGAGGGTGTC TACGTCAACA CGTACGGCCG GATCATCAAG 
GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTGG CCTACATCTG CTCCAACCAG 
ATCATGGGCT GGGGAGAGAA AGCCATTGAG ATCCGCTCCG TGGAGACGGG CCACCTGGAT 
GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAACGACAAG 
GTATTTTTCG CCTCGGTCCG CTCCGGGGGC AGCAGCCAAG TTTACTTCAT GACCTTGAAC 
CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980146.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835914.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X7, mRNA.

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CloneID	OBa226354
Clone ID Related Accession (Same CDS sequence)	XM_024980146.1
Accession Version	XM_024980146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCAGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835914.1
CDS	257..4276
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980146.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980146.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCAGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980147.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835915.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa226355
Clone ID Related Accession (Same CDS sequence)	XM_024980147.1
Accession Version	XM_024980147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCGATG GAGACACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT GGAAGAGATA 
GCCGGGCCCC AGACCCCCTA CGGGGGTGGC ACCATGGTGG TCCAGCGAAC CCCCGAGGAG 
GAGCGCAGCC TGTTGCATGC CGACAGCAAC GGCTACACAA ACCTGCCAGA CGTGGTCCAG 
CCCAGCCACT CGCCCACTGA GAGCAGCAAA GGTCAAAGCC CCCCCTTGAA GGATGGAGGC 
AGTGACTACC AGTCTCGTGG GCTGGTAAAA GCCCCTGGCA AGAGCTCATT CACGATGTTT 
GTGGACCTGG GGATCTACCA GCCCGGAGGC AGTGGGGACA CCATTCCCAT TACAGCTCTG 
GTGGGTGGAG AGGGCAGCCG GCTCGATCAG CTACAGTACG ACGTGAGGAA AGGCTCTGTA 
GTCAACGTGA ACCCCACCAA CACCCGGGCC CACAGCGAAA CCCCCGAGAT TCGGAAGTAC 
AAGAAGCGGT TCAATTCTGA GATCCTCTGT GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG 
GGCACGGAGA ATGGGCTGAT GTTGCTGGAC CGCAGCGGGC AGGGCAAGGT GTATGGACTC 
ATCGGCCGGC GGCGCTTCCA GCAAATGGAC GTGCTGGAGG GACTCAACTT GCTCATCACC 
ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT 
CTGCACAATG ACCCGGAAGT GGAGAAGAAG CAAGGCTGGA CCACTGTGGG GGACATGGAG 
GGCTGCGGGC ACTACCGCGT GGTGAAATAC GAACGCATCA AGTTCCTAGT CATCGCCCTG 
AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCCAAACCTT ACCACAAATT CATGGCTTTC 
AAGTCCTTTG CTGACCTCCC GCACCGCCCT CTGCTGGTGG ACCTGACCGT AGAGGAGGGC 
CAGCGGCTCA AGGTCATCTA TGGCTCCAGC GCCGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGGAACAGCT ACGACATCTA CATCCCTGTA CATATCCAGA GCCAGATCAC ACCCCACGCC 
ATCATCTTCC TCCCCAACAC GGACGGCATG GAGATGCTGC TGTGCTATGA GGATGAGGGT 
GTCTACGTCA ACACGTACGG CCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG 
CCCACCTCTG TGGCCTACAT CTGCTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CCGTGGAGAC GGGCCACCTG GATGGGGTCT TCATGCACAA ACGAGCCCAG 
AGGCTCAAGT TCCTGTGTGA GCGGAACGAC AAGGTATTTT TCGCCTCGGT CCGCTCCGGG 
GGCAGCAGCC AAGTTTACTT CATGACCTTG AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835915.1
CDS	227..4243
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980147.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980147.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCGATG GAGACACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT GGAAGAGATA 
GCCGGGCCCC AGACCCCCTA CGGGGGTGGC ACCATGGTGG TCCAGCGAAC CCCCGAGGAG 
GAGCGCAGCC TGTTGCATGC CGACAGCAAC GGCTACACAA ACCTGCCAGA CGTGGTCCAG 
CCCAGCCACT CGCCCACTGA GAGCAGCAAA GGTCAAAGCC CCCCCTTGAA GGATGGAGGC 
AGTGACTACC AGTCTCGTGG GCTGGTAAAA GCCCCTGGCA AGAGCTCATT CACGATGTTT 
GTGGACCTGG GGATCTACCA GCCCGGAGGC AGTGGGGACA CCATTCCCAT TACAGCTCTG 
GTGGGTGGAG AGGGCAGCCG GCTCGATCAG CTACAGTACG ACGTGAGGAA AGGCTCTGTA 
GTCAACGTGA ACCCCACCAA CACCCGGGCC CACAGCGAAA CCCCCGAGAT TCGGAAGTAC 
AAGAAGCGGT TCAATTCTGA GATCCTCTGT GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG 
GGCACGGAGA ATGGGCTGAT GTTGCTGGAC CGCAGCGGGC AGGGCAAGGT GTATGGACTC 
ATCGGCCGGC GGCGCTTCCA GCAAATGGAC GTGCTGGAGG GACTCAACTT GCTCATCACC 
ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT 
CTGCACAATG ACCCGGAAGT GGAGAAGAAG CAAGGCTGGA CCACTGTGGG GGACATGGAG 
GGCTGCGGGC ACTACCGCGT GGTGAAATAC GAACGCATCA AGTTCCTAGT CATCGCCCTG 
AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCCAAACCTT ACCACAAATT CATGGCTTTC 
AAGTCCTTTG CTGACCTCCC GCACCGCCCT CTGCTGGTGG ACCTGACCGT AGAGGAGGGC 
CAGCGGCTCA AGGTCATCTA TGGCTCCAGC GCCGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGGAACAGCT ACGACATCTA CATCCCTGTA CATATCCAGA GCCAGATCAC ACCCCACGCC 
ATCATCTTCC TCCCCAACAC GGACGGCATG GAGATGCTGC TGTGCTATGA GGATGAGGGT 
GTCTACGTCA ACACGTACGG CCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG 
CCCACCTCTG TGGCCTACAT CTGCTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CCGTGGAGAC GGGCCACCTG GATGGGGTCT TCATGCACAA ACGAGCCCAG 
AGGCTCAAGT TCCTGTGTGA GCGGAACGAC AAGGTATTTT TCGCCTCGGT CCGCTCCGGG 
GGCAGCAGCC AAGTTTACTT CATGACCTTG AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980148.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835916.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa226356
Clone ID Related Accession (Same CDS sequence)	XM_024980148.1
Accession Version	XM_024980148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835916.1
CDS	227..4222
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980148.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980148.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835917.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X10, mRNA.

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CloneID	OBa226357
Clone ID Related Accession (Same CDS sequence)	XM_024980149.1
Accession Version	XM_024980149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835917.1
CDS	227..4222
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980149.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835918.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X11, mRNA.

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CloneID	OBa226358
Clone ID Related Accession (Same CDS sequence)	XM_024980150.1
Accession Version	XM_024980150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835918.1
CDS	227..4219
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980150.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835919.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa226359
Clone ID Related Accession (Same CDS sequence)	XM_024980151.1
Accession Version	XM_024980151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835919.1
CDS	227..4219
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980151.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835920.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa226360
Clone ID Related Accession (Same CDS sequence)	XM_024980152.1
Accession Version	XM_024980152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCAG CGATGGAGAC ACAGACAGCG TCAGCACCAT GGTGGTCCAC 
GACGTGGAAG AGATAGCCGG GCCCCAGACC CCCTACGGGG GTGGCACCAT GGTGGTCCAG 
CGAACCCCCG AGGAGGAGCG CAGCCTGTTG CATGCCGACA GCAACGGCTA CACAAACCTG 
CCAGACGTGG TCCAGCCCAG CCACTCGCCC ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC 
TTGAAGGATG GAGGCAGTGA CTACCAGTCT CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC 
TCATTCACGA TGTTTGTGGA CCTGGGGATC TACCAGCCCG GAGGCAGTGG GGACACCATT 
CCCATTACAG CTCTGGTGGG TGGAGAGGGC AGCCGGCTCG ATCAGCTACA GTACGACGTG 
AGGAAAGGCT CTGTAGTCAA CGTGAACCCC ACCAACACCC GGGCCCACAG CGAAACCCCC 
GAGATTCGGA AGTACAAGAA GCGGTTCAAT TCTGAGATCC TCTGTGCGGC CCTTTGGGGG 
GTCAACCTGC TGGTGGGCAC GGAGAATGGG CTGATGTTGC TGGACCGCAG CGGGCAGGGC 
AAGGTGTATG GACTCATCGG CCGGCGGCGC TTCCAGCAAA TGGACGTGCT GGAGGGACTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG 
CTCCGGAACA AGATTCTGCA CAATGACCCG GAAGTGGAGA AGAAGCAAGG CTGGACCACT 
GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGCGTGGTGA AATACGAACG CATCAAGTTC 
CTAGTCATCG CCCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCCAA ACCTTACCAC 
AAATTCATGG CTTTCAAGTC CTTTGCTGAC CTCCCGCACC GCCCTCTGCT GGTGGACCTG 
ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGCGCCGG CTTCCACGCT 
GTGGATGTGG ACTCGGGGAA CAGCTACGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACGGACG GCATGGAGAT GCTGCTGTGC 
TATGAGGATG AGGGTGTCTA CGTCAACACG TACGGCCGGA TCATCAAGGA CGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCCGTG GAGACGGGCC ACCTGGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ACGACAAGGT ATTTTTCGCC 
TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT TACTTCATGA CCTTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835920.1
CDS	227..4198
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980152.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCAG CGATGGAGAC ACAGACAGCG TCAGCACCAT GGTGGTCCAC 
GACGTGGAAG AGATAGCCGG GCCCCAGACC CCCTACGGGG GTGGCACCAT GGTGGTCCAG 
CGAACCCCCG AGGAGGAGCG CAGCCTGTTG CATGCCGACA GCAACGGCTA CACAAACCTG 
CCAGACGTGG TCCAGCCCAG CCACTCGCCC ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC 
TTGAAGGATG GAGGCAGTGA CTACCAGTCT CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC 
TCATTCACGA TGTTTGTGGA CCTGGGGATC TACCAGCCCG GAGGCAGTGG GGACACCATT 
CCCATTACAG CTCTGGTGGG TGGAGAGGGC AGCCGGCTCG ATCAGCTACA GTACGACGTG 
AGGAAAGGCT CTGTAGTCAA CGTGAACCCC ACCAACACCC GGGCCCACAG CGAAACCCCC 
GAGATTCGGA AGTACAAGAA GCGGTTCAAT TCTGAGATCC TCTGTGCGGC CCTTTGGGGG 
GTCAACCTGC TGGTGGGCAC GGAGAATGGG CTGATGTTGC TGGACCGCAG CGGGCAGGGC 
AAGGTGTATG GACTCATCGG CCGGCGGCGC TTCCAGCAAA TGGACGTGCT GGAGGGACTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG 
CTCCGGAACA AGATTCTGCA CAATGACCCG GAAGTGGAGA AGAAGCAAGG CTGGACCACT 
GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGCGTGGTGA AATACGAACG CATCAAGTTC 
CTAGTCATCG CCCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCCAA ACCTTACCAC 
AAATTCATGG CTTTCAAGTC CTTTGCTGAC CTCCCGCACC GCCCTCTGCT GGTGGACCTG 
ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGCGCCGG CTTCCACGCT 
GTGGATGTGG ACTCGGGGAA CAGCTACGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACGGACG GCATGGAGAT GCTGCTGTGC 
TATGAGGATG AGGGTGTCTA CGTCAACACG TACGGCCGGA TCATCAAGGA CGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCCGTG GAGACGGGCC ACCTGGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ACGACAAGGT ATTTTTCGCC 
TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT TACTTCATGA CCTTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835921.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X14, mRNA.

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CloneID	OBa226361
Clone ID Related Accession (Same CDS sequence)	XM_024980153.1
Accession Version	XM_024980153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835921.1
CDS	227..4195
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980153.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT 
GCAGCGCCTG TACCCCGCTC CCAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835922.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa226362
Clone ID Related Accession (Same CDS sequence)	XM_024980154.1
Accession Version	XM_024980154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCAGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835922.1
CDS	227..4186
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980154.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCAGCG ATGGAGACAC AGACAGCGTC AGCACCATGG TGGTCCACGA CGTGGAAGAG 
ATAGCCGGGC CCCAGACCCC CTACGGGGGT GGCACCATGG TGGTCCAGCG AACCCCCGAG 
GAGGAGCGCA GCCTGTTGCA TGCCGACAGC AACGGCTACA CAAACCTGCC AGACGTGGTC 
CAGCCCAGCC ACTCGCCCAC TGAGAGCAGC AAAGGTCAAA GCCCCCCCTT GAAGGATGGA 
GGCAGTGACT ACCAGTCTCG TGGGCTGGTA AAAGCCCCTG GCAAGAGCTC ATTCACGATG 
TTTGTGGACC TGGGGATCTA CCAGCCCGGA GGCAGTGGGG ACACCATTCC CATTACAGCT 
CTGGTGGGTG GAGAGGGCAG CCGGCTCGAT CAGCTACAGT ACGACGTGAG GAAAGGCTCT 
GTAGTCAACG TGAACCCCAC CAACACCCGG GCCCACAGCG AAACCCCCGA GATTCGGAAG 
TACAAGAAGC GGTTCAATTC TGAGATCCTC TGTGCGGCCC TTTGGGGGGT CAACCTGCTG 
GTGGGCACGG AGAATGGGCT GATGTTGCTG GACCGCAGCG GGCAGGGCAA GGTGTATGGA 
CTCATCGGCC GGCGGCGCTT CCAGCAAATG GACGTGCTGG AGGGACTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTGTATTACC TGTCCTGGCT CCGGAACAAG 
ATTCTGCACA ATGACCCGGA AGTGGAGAAG AAGCAAGGCT GGACCACTGT GGGGGACATG 
GAGGGCTGCG GGCACTACCG CGTGGTGAAA TACGAACGCA TCAAGTTCCT AGTCATCGCC 
CTGAAGAACT CTGTGGAGGT GTATGCCTGG GCCCCCAAAC CTTACCACAA ATTCATGGCT 
TTCAAGTCCT TTGCTGACCT CCCGCACCGC CCTCTGCTGG TGGACCTGAC CGTAGAGGAG 
GGCCAGCGGC TCAAGGTCAT CTATGGCTCC AGCGCCGGCT TCCACGCTGT GGATGTGGAC 
TCGGGGAACA GCTACGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACGGACGGC ATGGAGATGC TGCTGTGCTA TGAGGATGAG 
GGTGTCTACG TCAACACGTA CGGCCGGATC ATCAAGGACG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATCA TGGGCTGGGG AGAGAAAGCC 
ATTGAGATCC GCTCCGTGGA GACGGGCCAC CTGGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGGAAC GACAAGGTAT TTTTCGCCTC GGTCCGCTCC 
GGGGGCAGCA GCCAAGTTTA CTTCATGACC TTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980155.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835923.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa226363
Clone ID Related Accession (Same CDS sequence)	XM_024980155.1
Accession Version	XM_024980155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCGATG GAGACACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT GGAAGAGATA 
GCCGGGCCCC AGACCCCCTA CGGGGGTGGC ACCATGGTGG TCCAGCGAAC CCCCGAGGAG 
GAGCGCAGCC TGTTGCATGC CGACAGCAAC GGCTACACAA ACCTGCCAGA CGTGGTCCAG 
CCCAGCCACT CGCCCACTGA GAGCAGCAAA GGTCAAAGCC CCCCCTTGAA GGATGGAGGC 
AGTGACTACC AGTCTCGTGG GCTGGTAAAA GCCCCTGGCA AGAGCTCATT CACGATGTTT 
GTGGACCTGG GGATCTACCA GCCCGGAGGC AGTGGGGACA CCATTCCCAT TACAGCTCTG 
GTGGGTGGAG AGGGCAGCCG GCTCGATCAG CTACAGTACG ACGTGAGGAA AGGCTCTGTA 
GTCAACGTGA ACCCCACCAA CACCCGGGCC CACAGCGAAA CCCCCGAGAT TCGGAAGTAC 
AAGAAGCGGT TCAATTCTGA GATCCTCTGT GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG 
GGCACGGAGA ATGGGCTGAT GTTGCTGGAC CGCAGCGGGC AGGGCAAGGT GTATGGACTC 
ATCGGCCGGC GGCGCTTCCA GCAAATGGAC GTGCTGGAGG GACTCAACTT GCTCATCACC 
ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT 
CTGCACAATG ACCCGGAAGT GGAGAAGAAG CAAGGCTGGA CCACTGTGGG GGACATGGAG 
GGCTGCGGGC ACTACCGCGT GGTGAAATAC GAACGCATCA AGTTCCTAGT CATCGCCCTG 
AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCCAAACCTT ACCACAAATT CATGGCTTTC 
AAGTCCTTTG CTGACCTCCC GCACCGCCCT CTGCTGGTGG ACCTGACCGT AGAGGAGGGC 
CAGCGGCTCA AGGTCATCTA TGGCTCCAGC GCCGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGGAACAGCT ACGACATCTA CATCCCTGTA CATATCCAGA GCCAGATCAC ACCCCACGCC 
ATCATCTTCC TCCCCAACAC GGACGGCATG GAGATGCTGC TGTGCTATGA GGATGAGGGT 
GTCTACGTCA ACACGTACGG CCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG 
CCCACCTCTG TGGCCTACAT CTGCTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CCGTGGAGAC GGGCCACCTG GATGGGGTCT TCATGCACAA ACGAGCCCAG 
AGGCTCAAGT TCCTGTGTGA GCGGAACGAC AAGGTATTTT TCGCCTCGGT CCGCTCCGGG 
GGCAGCAGCC AAGTTTACTT CATGACCTTG AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835923.1
CDS	227..4183
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980155.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980155.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAAGC 
TATAAGCGAG CCATTGGTGA GGATTTCGTC TTGCTGAAAG AGCGGACCCT GGACGAGGCC 
CCCCGGCCTC CCAAGAAGGC GATGGACTAC TCATCGTCCA GCGAGGAGGT GGAGAGCAGC 
GAGGACGAGG AGGAGGAGAG CAACGGCGAG CCGTCAGAGG GGAGCAGGGA CGCCCCTGGG 
GCCCGCGATG GAGACACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT GGAAGAGATA 
GCCGGGCCCC AGACCCCCTA CGGGGGTGGC ACCATGGTGG TCCAGCGAAC CCCCGAGGAG 
GAGCGCAGCC TGTTGCATGC CGACAGCAAC GGCTACACAA ACCTGCCAGA CGTGGTCCAG 
CCCAGCCACT CGCCCACTGA GAGCAGCAAA GGTCAAAGCC CCCCCTTGAA GGATGGAGGC 
AGTGACTACC AGTCTCGTGG GCTGGTAAAA GCCCCTGGCA AGAGCTCATT CACGATGTTT 
GTGGACCTGG GGATCTACCA GCCCGGAGGC AGTGGGGACA CCATTCCCAT TACAGCTCTG 
GTGGGTGGAG AGGGCAGCCG GCTCGATCAG CTACAGTACG ACGTGAGGAA AGGCTCTGTA 
GTCAACGTGA ACCCCACCAA CACCCGGGCC CACAGCGAAA CCCCCGAGAT TCGGAAGTAC 
AAGAAGCGGT TCAATTCTGA GATCCTCTGT GCGGCCCTTT GGGGGGTCAA CCTGCTGGTG 
GGCACGGAGA ATGGGCTGAT GTTGCTGGAC CGCAGCGGGC AGGGCAAGGT GTATGGACTC 
ATCGGCCGGC GGCGCTTCCA GCAAATGGAC GTGCTGGAGG GACTCAACTT GCTCATCACC 
ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT 
CTGCACAATG ACCCGGAAGT GGAGAAGAAG CAAGGCTGGA CCACTGTGGG GGACATGGAG 
GGCTGCGGGC ACTACCGCGT GGTGAAATAC GAACGCATCA AGTTCCTAGT CATCGCCCTG 
AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCCAAACCTT ACCACAAATT CATGGCTTTC 
AAGTCCTTTG CTGACCTCCC GCACCGCCCT CTGCTGGTGG ACCTGACCGT AGAGGAGGGC 
CAGCGGCTCA AGGTCATCTA TGGCTCCAGC GCCGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGGAACAGCT ACGACATCTA CATCCCTGTA CATATCCAGA GCCAGATCAC ACCCCACGCC 
ATCATCTTCC TCCCCAACAC GGACGGCATG GAGATGCTGC TGTGCTATGA GGATGAGGGT 
GTCTACGTCA ACACGTACGG CCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG 
CCCACCTCTG TGGCCTACAT CTGCTCCAAC CAGATCATGG GCTGGGGAGA GAAAGCCATT 
GAGATCCGCT CCGTGGAGAC GGGCCACCTG GATGGGGTCT TCATGCACAA ACGAGCCCAG 
AGGCTCAAGT TCCTGTGTGA GCGGAACGAC AAGGTATTTT TCGCCTCGGT CCGCTCCGGG 
GGCAGCAGCC AAGTTTACTT CATGACCTTG AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980156.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835924.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X17, mRNA.

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CloneID	OBa226364
Clone ID Related Accession (Same CDS sequence)	XM_024980156.1
Accession Version	XM_024980156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835924.1
CDS	227..4162
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980156.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCAGCGATGG AGACACAGAC 
AGCGTCAGCA CCATGGTGGT CCACGACGTG GAAGAGATAG CCGGGCCCCA GACCCCCTAC 
GGGGGTGGCA CCATGGTGGT CCAGCGAACC CCCGAGGAGG AGCGCAGCCT GTTGCATGCC 
GACAGCAACG GCTACACAAA CCTGCCAGAC GTGGTCCAGC CCAGCCACTC GCCCACTGAG 
AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG GATGGAGGCA GTGACTACCA GTCTCGTGGG 
CTGGTAAAAG CCCCTGGCAA GAGCTCATTC ACGATGTTTG TGGACCTGGG GATCTACCAG 
CCCGGAGGCA GTGGGGACAC CATTCCCATT ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG 
CTCGATCAGC TACAGTACGA CGTGAGGAAA GGCTCTGTAG TCAACGTGAA CCCCACCAAC 
ACCCGGGCCC ACAGCGAAAC CCCCGAGATT CGGAAGTACA AGAAGCGGTT CAATTCTGAG 
ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC CTGCTGGTGG GCACGGAGAA TGGGCTGATG 
TTGCTGGACC GCAGCGGGCA GGGCAAGGTG TATGGACTCA TCGGCCGGCG GCGCTTCCAG 
CAAATGGACG TGCTGGAGGG ACTCAACTTG CTCATCACCA TCTCAGGGAA AAGGAACAAA 
CTGCGGGTGT ATTACCTGTC CTGGCTCCGG AACAAGATTC TGCACAATGA CCCGGAAGTG 
GAGAAGAAGC AAGGCTGGAC CACTGTGGGG GACATGGAGG GCTGCGGGCA CTACCGCGTG 
GTGAAATACG AACGCATCAA GTTCCTAGTC ATCGCCCTGA AGAACTCTGT GGAGGTGTAT 
GCCTGGGCCC CCAAACCTTA CCACAAATTC ATGGCTTTCA AGTCCTTTGC TGACCTCCCG 
CACCGCCCTC TGCTGGTGGA CCTGACCGTA GAGGAGGGCC AGCGGCTCAA GGTCATCTAT 
GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT GTGGACTCGG GGAACAGCTA CGACATCTAC 
ATCCCTGTAC ATATCCAGAG CCAGATCACA CCCCACGCCA TCATCTTCCT CCCCAACACG 
GACGGCATGG AGATGCTGCT GTGCTATGAG GATGAGGGTG TCTACGTCAA CACGTACGGC 
CGGATCATCA AGGACGTGGT GCTGCAGTGG GGAGAGATGC CCACCTCTGT GGCCTACATC 
TGCTCCAACC AGATCATGGG CTGGGGAGAG AAAGCCATTG AGATCCGCTC CGTGGAGACG 
GGCCACCTGG ATGGGGTCTT CATGCACAAA CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG 
CGGAACGACA AGGTATTTTT CGCCTCGGTC CGCTCCGGGG GCAGCAGCCA AGTTTACTTC 
ATGACCTTGA ACCGTAACTG CATCATGAAC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980157.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835925.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa226365
Clone ID Related Accession (Same CDS sequence)	XM_024980157.1
Accession Version	XM_024980157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835925.1
CDS	227..4159
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980157.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980157.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCCAT TGGTGAGGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG 
CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980158.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835926.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa226366
Clone ID Related Accession (Same CDS sequence)	XM_024980158.1
Accession Version	XM_024980158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCAG CGATGGAGAC ACAGACAGCG TCAGCACCAT GGTGGTCCAC 
GACGTGGAAG AGATAGCCGG GCCCCAGACC CCCTACGGGG GTGGCACCAT GGTGGTCCAG 
CGAACCCCCG AGGAGGAGCG CAGCCTGTTG CATGCCGACA GCAACGGCTA CACAAACCTG 
CCAGACGTGG TCCAGCCCAG CCACTCGCCC ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC 
TTGAAGGATG GAGGCAGTGA CTACCAGTCT CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC 
TCATTCACGA TGTTTGTGGA CCTGGGGATC TACCAGCCCG GAGGCAGTGG GGACACCATT 
CCCATTACAG CTCTGGTGGG TGGAGAGGGC AGCCGGCTCG ATCAGCTACA GTACGACGTG 
AGGAAAGGCT CTGTAGTCAA CGTGAACCCC ACCAACACCC GGGCCCACAG CGAAACCCCC 
GAGATTCGGA AGTACAAGAA GCGGTTCAAT TCTGAGATCC TCTGTGCGGC CCTTTGGGGG 
GTCAACCTGC TGGTGGGCAC GGAGAATGGG CTGATGTTGC TGGACCGCAG CGGGCAGGGC 
AAGGTGTATG GACTCATCGG CCGGCGGCGC TTCCAGCAAA TGGACGTGCT GGAGGGACTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG 
CTCCGGAACA AGATTCTGCA CAATGACCCG GAAGTGGAGA AGAAGCAAGG CTGGACCACT 
GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGCGTGGTGA AATACGAACG CATCAAGTTC 
CTAGTCATCG CCCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCCAA ACCTTACCAC 
AAATTCATGG CTTTCAAGTC CTTTGCTGAC CTCCCGCACC GCCCTCTGCT GGTGGACCTG 
ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGCGCCGG CTTCCACGCT 
GTGGATGTGG ACTCGGGGAA CAGCTACGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACGGACG GCATGGAGAT GCTGCTGTGC 
TATGAGGATG AGGGTGTCTA CGTCAACACG TACGGCCGGA TCATCAAGGA CGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCCGTG GAGACGGGCC ACCTGGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ACGACAAGGT ATTTTTCGCC 
TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT TACTTCATGA CCTTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835926.1
CDS	227..4138
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980158.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980158.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAGTATATCC GTCACAGGCT AGAGGAGGAG 
CAGCGACAGC TCGAGATCCT TCAGCAACAG CTGCTCCAGG AACAGGCCCT GCTGCTGGAA 
TACAAGCGGA AGCAGCTGGA GGAGCAACGG CAGTCCGAGC GCCTGCAGAG GCAGCTGCAG 
CAGGAGCACG CCTACCTCAA GTCCCTGCAG CAGCAGCAGC AACAGCAGCA GCTTCAGAAA 
CAGCAGCAGA TCCTGCCCGG GGACCGGAAG CCCCTGTATC ACTACGGTCG GGGCATCAAC 
CCTGCCGACA AGCCAGCCTG GGCCCGAGAG GTGGAAGAGA GAACAAGGAT GAACAAACAG 
CAGAACTCTC CCTTGGCCAA GAGCAAGCCA GGCAGCACAG GGCCTGAGCC CCCCATCCCC 
CAGGCCTCCC CCGGGCCCCC AGGACCCCTT TCCCAAACTC CTCCTATGCA GAGGCCGGTG 
GAGCCCCAGG AGGGACCGCA CAAGTCCCTG CAGGACCAAC CCACCCGAAA CCTGGCTGCC 
TTCCCCGCCT CCCACGACCC CGACCCCGCC GTGCCCGCAC CCACCGCCAC GCCGAGTGCC 
CGAGGAGCCG TCATCCGGCA GAACTCAGAC CCCACTTCCG AAGGGCCTGG CCCCAGCCCG 
AATCCCCCAG CCTGGGTCCG GCCTGATAAT GAGGCCCCTC CAAAGGTGCC TCAGAGGACC 
TCATCAATCG CCACTGCCCT TAACACCAGT GGGGCCGGAG GGTCCCGGCC AGCGCAGGCG 
GTCCGTGCTA GTAACCCCGA CCTCAGGAGG AGCGACCCCG GCTGGGAGCG CTCAGACAGC 
GTCCTCCCGG CCTCTCATGG GCACCTCCCA CAAGCCGGCT CGCTGGAGCG GAACCGGGTG 
GGAGCCTCCT CCAAACTGGA CAACTCCCCT GTGCTCTCCC CCGGAAACAA AACCAAGCCT 
GATGACCACC GCTCGCGGCC AGGCCGGCCC GCAGATTTCG TCTTGCTGAA AGAGCGGACC 
CTGGACGAGG CCCCCCGGCC TCCCAAGAAG GCGATGGACT ACTCATCGTC CAGCGAGGAG 
GTGGAGAGCA GCGAGGACGA GGAGGAGGAG AGCAACGGCG AGCCGTCAGA GGGGAGCAGG 
GACGCCCCTG GGGCCCGCAG CGATGGAGAC ACAGACAGCG TCAGCACCAT GGTGGTCCAC 
GACGTGGAAG AGATAGCCGG GCCCCAGACC CCCTACGGGG GTGGCACCAT GGTGGTCCAG 
CGAACCCCCG AGGAGGAGCG CAGCCTGTTG CATGCCGACA GCAACGGCTA CACAAACCTG 
CCAGACGTGG TCCAGCCCAG CCACTCGCCC ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC 
TTGAAGGATG GAGGCAGTGA CTACCAGTCT CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC 
TCATTCACGA TGTTTGTGGA CCTGGGGATC TACCAGCCCG GAGGCAGTGG GGACACCATT 
CCCATTACAG CTCTGGTGGG TGGAGAGGGC AGCCGGCTCG ATCAGCTACA GTACGACGTG 
AGGAAAGGCT CTGTAGTCAA CGTGAACCCC ACCAACACCC GGGCCCACAG CGAAACCCCC 
GAGATTCGGA AGTACAAGAA GCGGTTCAAT TCTGAGATCC TCTGTGCGGC CCTTTGGGGG 
GTCAACCTGC TGGTGGGCAC GGAGAATGGG CTGATGTTGC TGGACCGCAG CGGGCAGGGC 
AAGGTGTATG GACTCATCGG CCGGCGGCGC TTCCAGCAAA TGGACGTGCT GGAGGGACTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG 
CTCCGGAACA AGATTCTGCA CAATGACCCG GAAGTGGAGA AGAAGCAAGG CTGGACCACT 
GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGCGTGGTGA AATACGAACG CATCAAGTTC 
CTAGTCATCG CCCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCCAA ACCTTACCAC 
AAATTCATGG CTTTCAAGTC CTTTGCTGAC CTCCCGCACC GCCCTCTGCT GGTGGACCTG 
ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGCGCCGG CTTCCACGCT 
GTGGATGTGG ACTCGGGGAA CAGCTACGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACGGACG GCATGGAGAT GCTGCTGTGC 
TATGAGGATG AGGGTGTCTA CGTCAACACG TACGGCCGGA TCATCAAGGA CGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT CATGGGCTGG 
GGAGAGAAAG CCATTGAGAT CCGCTCCGTG GAGACGGGCC ACCTGGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ACGACAAGGT ATTTTTCGCC 
TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT TACTTCATGA CCTTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980159.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835927.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X20, mRNA.

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CloneID	OBa226367
Clone ID Related Accession (Same CDS sequence)	XM_024980159.1
Accession Version	XM_024980159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCAGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835927.1
CDS	227..4135
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980159.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980159.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCAGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980160.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835928.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa226368
Clone ID Related Accession (Same CDS sequence)	XM_024980160.1
Accession Version	XM_024980160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCGATGG AGACACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTG 
GAAGAGATAG CCGGGCCCCA GACCCCCTAC GGGGGTGGCA CCATGGTGGT CCAGCGAACC 
CCCGAGGAGG AGCGCAGCCT GTTGCATGCC GACAGCAACG GCTACACAAA CCTGCCAGAC 
GTGGTCCAGC CCAGCCACTC GCCCACTGAG AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG 
GATGGAGGCA GTGACTACCA GTCTCGTGGG CTGGTAAAAG CCCCTGGCAA GAGCTCATTC 
ACGATGTTTG TGGACCTGGG GATCTACCAG CCCGGAGGCA GTGGGGACAC CATTCCCATT 
ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG CTCGATCAGC TACAGTACGA CGTGAGGAAA 
GGCTCTGTAG TCAACGTGAA CCCCACCAAC ACCCGGGCCC ACAGCGAAAC CCCCGAGATT 
CGGAAGTACA AGAAGCGGTT CAATTCTGAG ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC 
CTGCTGGTGG GCACGGAGAA TGGGCTGATG TTGCTGGACC GCAGCGGGCA GGGCAAGGTG 
TATGGACTCA TCGGCCGGCG GCGCTTCCAG CAAATGGACG TGCTGGAGGG ACTCAACTTG 
CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG 
AACAAGATTC TGCACAATGA CCCGGAAGTG GAGAAGAAGC AAGGCTGGAC CACTGTGGGG 
GACATGGAGG GCTGCGGGCA CTACCGCGTG GTGAAATACG AACGCATCAA GTTCCTAGTC 
ATCGCCCTGA AGAACTCTGT GGAGGTGTAT GCCTGGGCCC CCAAACCTTA CCACAAATTC 
ATGGCTTTCA AGTCCTTTGC TGACCTCCCG CACCGCCCTC TGCTGGTGGA CCTGACCGTA 
GAGGAGGGCC AGCGGCTCAA GGTCATCTAT GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT 
GTGGACTCGG GGAACAGCTA CGACATCTAC ATCCCTGTAC ATATCCAGAG CCAGATCACA 
CCCCACGCCA TCATCTTCCT CCCCAACACG GACGGCATGG AGATGCTGCT GTGCTATGAG 
GATGAGGGTG TCTACGTCAA CACGTACGGC CGGATCATCA AGGACGTGGT GCTGCAGTGG 
GGAGAGATGC CCACCTCTGT GGCCTACATC TGCTCCAACC AGATCATGGG CTGGGGAGAG 
AAAGCCATTG AGATCCGCTC CGTGGAGACG GGCCACCTGG ATGGGGTCTT CATGCACAAA 
CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG CGGAACGACA AGGTATTTTT CGCCTCGGTC 
CGCTCCGGGG GCAGCAGCCA AGTTTACTTC ATGACCTTGA ACCGTAACTG CATCATGAAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835928.1
CDS	227..4132
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980160.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980160.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCGATGG AGACACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTG 
GAAGAGATAG CCGGGCCCCA GACCCCCTAC GGGGGTGGCA CCATGGTGGT CCAGCGAACC 
CCCGAGGAGG AGCGCAGCCT GTTGCATGCC GACAGCAACG GCTACACAAA CCTGCCAGAC 
GTGGTCCAGC CCAGCCACTC GCCCACTGAG AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG 
GATGGAGGCA GTGACTACCA GTCTCGTGGG CTGGTAAAAG CCCCTGGCAA GAGCTCATTC 
ACGATGTTTG TGGACCTGGG GATCTACCAG CCCGGAGGCA GTGGGGACAC CATTCCCATT 
ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG CTCGATCAGC TACAGTACGA CGTGAGGAAA 
GGCTCTGTAG TCAACGTGAA CCCCACCAAC ACCCGGGCCC ACAGCGAAAC CCCCGAGATT 
CGGAAGTACA AGAAGCGGTT CAATTCTGAG ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC 
CTGCTGGTGG GCACGGAGAA TGGGCTGATG TTGCTGGACC GCAGCGGGCA GGGCAAGGTG 
TATGGACTCA TCGGCCGGCG GCGCTTCCAG CAAATGGACG TGCTGGAGGG ACTCAACTTG 
CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG 
AACAAGATTC TGCACAATGA CCCGGAAGTG GAGAAGAAGC AAGGCTGGAC CACTGTGGGG 
GACATGGAGG GCTGCGGGCA CTACCGCGTG GTGAAATACG AACGCATCAA GTTCCTAGTC 
ATCGCCCTGA AGAACTCTGT GGAGGTGTAT GCCTGGGCCC CCAAACCTTA CCACAAATTC 
ATGGCTTTCA AGTCCTTTGC TGACCTCCCG CACCGCCCTC TGCTGGTGGA CCTGACCGTA 
GAGGAGGGCC AGCGGCTCAA GGTCATCTAT GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT 
GTGGACTCGG GGAACAGCTA CGACATCTAC ATCCCTGTAC ATATCCAGAG CCAGATCACA 
CCCCACGCCA TCATCTTCCT CCCCAACACG GACGGCATGG AGATGCTGCT GTGCTATGAG 
GATGAGGGTG TCTACGTCAA CACGTACGGC CGGATCATCA AGGACGTGGT GCTGCAGTGG 
GGAGAGATGC CCACCTCTGT GGCCTACATC TGCTCCAACC AGATCATGGG CTGGGGAGAG 
AAAGCCATTG AGATCCGCTC CGTGGAGACG GGCCACCTGG ATGGGGTCTT CATGCACAAA 
CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG CGGAACGACA AGGTATTTTT CGCCTCGGTC 
CGCTCCGGGG GCAGCAGCCA AGTTTACTTC ATGACCTTGA ACCGTAACTG CATCATGAAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980161.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835929.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa226369
Clone ID Related Accession (Same CDS sequence)	XM_024980161.1
Accession Version	XM_024980161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CAGCGATGGA 
GACACAGACA GCGTCAGCAC CATGGTGGTC CACGACGTGG AAGAGATAGC CGGGCCCCAG 
ACCCCCTACG GGGGTGGCAC CATGGTGGTC CAGCGAACCC CCGAGGAGGA GCGCAGCCTG 
TTGCATGCCG ACAGCAACGG CTACACAAAC CTGCCAGACG TGGTCCAGCC CAGCCACTCG 
CCCACTGAGA GCAGCAAAGG TCAAAGCCCC CCCTTGAAGG ATGGAGGCAG TGACTACCAG 
TCTCGTGGGC TGGTAAAAGC CCCTGGCAAG AGCTCATTCA CGATGTTTGT GGACCTGGGG 
ATCTACCAGC CCGGAGGCAG TGGGGACACC ATTCCCATTA CAGCTCTGGT GGGTGGAGAG 
GGCAGCCGGC TCGATCAGCT ACAGTACGAC GTGAGGAAAG GCTCTGTAGT CAACGTGAAC 
CCCACCAACA CCCGGGCCCA CAGCGAAACC CCCGAGATTC GGAAGTACAA GAAGCGGTTC 
AATTCTGAGA TCCTCTGTGC GGCCCTTTGG GGGGTCAACC TGCTGGTGGG CACGGAGAAT 
GGGCTGATGT TGCTGGACCG CAGCGGGCAG GGCAAGGTGT ATGGACTCAT CGGCCGGCGG 
CGCTTCCAGC AAATGGACGT GCTGGAGGGA CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTGTA TTACCTGTCC TGGCTCCGGA ACAAGATTCT GCACAATGAC 
CCGGAAGTGG AGAAGAAGCA AGGCTGGACC ACTGTGGGGG ACATGGAGGG CTGCGGGCAC 
TACCGCGTGG TGAAATACGA ACGCATCAAG TTCCTAGTCA TCGCCCTGAA GAACTCTGTG 
GAGGTGTATG CCTGGGCCCC CAAACCTTAC CACAAATTCA TGGCTTTCAA GTCCTTTGCT 
GACCTCCCGC ACCGCCCTCT GCTGGTGGAC CTGACCGTAG AGGAGGGCCA GCGGCTCAAG 
GTCATCTATG GCTCCAGCGC CGGCTTCCAC GCTGTGGATG TGGACTCGGG GAACAGCTAC 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACGG ACGGCATGGA GATGCTGCTG TGCTATGAGG ATGAGGGTGT CTACGTCAAC 
ACGTACGGCC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATCATGGGC TGGGGAGAGA AAGCCATTGA GATCCGCTCC 
GTGGAGACGG GCCACCTGGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GGAACGACAA GGTATTTTTC GCCTCGGTCC GCTCCGGGGG CAGCAGCCAA 
GTTTACTTCA TGACCTTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835929.1
CDS	227..4111
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980161.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980161.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CAGCGATGGA 
GACACAGACA GCGTCAGCAC CATGGTGGTC CACGACGTGG AAGAGATAGC CGGGCCCCAG 
ACCCCCTACG GGGGTGGCAC CATGGTGGTC CAGCGAACCC CCGAGGAGGA GCGCAGCCTG 
TTGCATGCCG ACAGCAACGG CTACACAAAC CTGCCAGACG TGGTCCAGCC CAGCCACTCG 
CCCACTGAGA GCAGCAAAGG TCAAAGCCCC CCCTTGAAGG ATGGAGGCAG TGACTACCAG 
TCTCGTGGGC TGGTAAAAGC CCCTGGCAAG AGCTCATTCA CGATGTTTGT GGACCTGGGG 
ATCTACCAGC CCGGAGGCAG TGGGGACACC ATTCCCATTA CAGCTCTGGT GGGTGGAGAG 
GGCAGCCGGC TCGATCAGCT ACAGTACGAC GTGAGGAAAG GCTCTGTAGT CAACGTGAAC 
CCCACCAACA CCCGGGCCCA CAGCGAAACC CCCGAGATTC GGAAGTACAA GAAGCGGTTC 
AATTCTGAGA TCCTCTGTGC GGCCCTTTGG GGGGTCAACC TGCTGGTGGG CACGGAGAAT 
GGGCTGATGT TGCTGGACCG CAGCGGGCAG GGCAAGGTGT ATGGACTCAT CGGCCGGCGG 
CGCTTCCAGC AAATGGACGT GCTGGAGGGA CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTGTA TTACCTGTCC TGGCTCCGGA ACAAGATTCT GCACAATGAC 
CCGGAAGTGG AGAAGAAGCA AGGCTGGACC ACTGTGGGGG ACATGGAGGG CTGCGGGCAC 
TACCGCGTGG TGAAATACGA ACGCATCAAG TTCCTAGTCA TCGCCCTGAA GAACTCTGTG 
GAGGTGTATG CCTGGGCCCC CAAACCTTAC CACAAATTCA TGGCTTTCAA GTCCTTTGCT 
GACCTCCCGC ACCGCCCTCT GCTGGTGGAC CTGACCGTAG AGGAGGGCCA GCGGCTCAAG 
GTCATCTATG GCTCCAGCGC CGGCTTCCAC GCTGTGGATG TGGACTCGGG GAACAGCTAC 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACGG ACGGCATGGA GATGCTGCTG TGCTATGAGG ATGAGGGTGT CTACGTCAAC 
ACGTACGGCC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATCATGGGC TGGGGAGAGA AAGCCATTGA GATCCGCTCC 
GTGGAGACGG GCCACCTGGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GGAACGACAA GGTATTTTTC GCCTCGGTCC GCTCCGGGGG CAGCAGCCAA 
GTTTACTTCA TGACCTTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980162.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835930.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X23, mRNA.

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CloneID	OBa226370
Clone ID Related Accession (Same CDS sequence)	XM_024980162.1
Accession Version	XM_024980162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CGATGGAGAC 
ACAGACAGCG TCAGCACCAT GGTGGTCCAC GACGTGGAAG AGATAGCCGG GCCCCAGACC 
CCCTACGGGG GTGGCACCAT GGTGGTCCAG CGAACCCCCG AGGAGGAGCG CAGCCTGTTG 
CATGCCGACA GCAACGGCTA CACAAACCTG CCAGACGTGG TCCAGCCCAG CCACTCGCCC 
ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC TTGAAGGATG GAGGCAGTGA CTACCAGTCT 
CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC TCATTCACGA TGTTTGTGGA CCTGGGGATC 
TACCAGCCCG GAGGCAGTGG GGACACCATT CCCATTACAG CTCTGGTGGG TGGAGAGGGC 
AGCCGGCTCG ATCAGCTACA GTACGACGTG AGGAAAGGCT CTGTAGTCAA CGTGAACCCC 
ACCAACACCC GGGCCCACAG CGAAACCCCC GAGATTCGGA AGTACAAGAA GCGGTTCAAT 
TCTGAGATCC TCTGTGCGGC CCTTTGGGGG GTCAACCTGC TGGTGGGCAC GGAGAATGGG 
CTGATGTTGC TGGACCGCAG CGGGCAGGGC AAGGTGTATG GACTCATCGG CCGGCGGCGC 
TTCCAGCAAA TGGACGTGCT GGAGGGACTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTGTATTA CCTGTCCTGG CTCCGGAACA AGATTCTGCA CAATGACCCG 
GAAGTGGAGA AGAAGCAAGG CTGGACCACT GTGGGGGACA TGGAGGGCTG CGGGCACTAC 
CGCGTGGTGA AATACGAACG CATCAAGTTC CTAGTCATCG CCCTGAAGAA CTCTGTGGAG 
GTGTATGCCT GGGCCCCCAA ACCTTACCAC AAATTCATGG CTTTCAAGTC CTTTGCTGAC 
CTCCCGCACC GCCCTCTGCT GGTGGACCTG ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC 
ATCTATGGCT CCAGCGCCGG CTTCCACGCT GTGGATGTGG ACTCGGGGAA CAGCTACGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACGGACG GCATGGAGAT GCTGCTGTGC TATGAGGATG AGGGTGTCTA CGTCAACACG 
TACGGCCGGA TCATCAAGGA CGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCCGTG 
GAGACGGGCC ACCTGGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGGA ACGACAAGGT ATTTTTCGCC TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT 
TACTTCATGA CCTTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835930.1
CDS	227..4108
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980162.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980162.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGAGC 
CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA TATGCAGCGC CTGTACCCCG CTCCCAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CGATGGAGAC 
ACAGACAGCG TCAGCACCAT GGTGGTCCAC GACGTGGAAG AGATAGCCGG GCCCCAGACC 
CCCTACGGGG GTGGCACCAT GGTGGTCCAG CGAACCCCCG AGGAGGAGCG CAGCCTGTTG 
CATGCCGACA GCAACGGCTA CACAAACCTG CCAGACGTGG TCCAGCCCAG CCACTCGCCC 
ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC TTGAAGGATG GAGGCAGTGA CTACCAGTCT 
CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC TCATTCACGA TGTTTGTGGA CCTGGGGATC 
TACCAGCCCG GAGGCAGTGG GGACACCATT CCCATTACAG CTCTGGTGGG TGGAGAGGGC 
AGCCGGCTCG ATCAGCTACA GTACGACGTG AGGAAAGGCT CTGTAGTCAA CGTGAACCCC 
ACCAACACCC GGGCCCACAG CGAAACCCCC GAGATTCGGA AGTACAAGAA GCGGTTCAAT 
TCTGAGATCC TCTGTGCGGC CCTTTGGGGG GTCAACCTGC TGGTGGGCAC GGAGAATGGG 
CTGATGTTGC TGGACCGCAG CGGGCAGGGC AAGGTGTATG GACTCATCGG CCGGCGGCGC 
TTCCAGCAAA TGGACGTGCT GGAGGGACTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTGTATTA CCTGTCCTGG CTCCGGAACA AGATTCTGCA CAATGACCCG 
GAAGTGGAGA AGAAGCAAGG CTGGACCACT GTGGGGGACA TGGAGGGCTG CGGGCACTAC 
CGCGTGGTGA AATACGAACG CATCAAGTTC CTAGTCATCG CCCTGAAGAA CTCTGTGGAG 
GTGTATGCCT GGGCCCCCAA ACCTTACCAC AAATTCATGG CTTTCAAGTC CTTTGCTGAC 
CTCCCGCACC GCCCTCTGCT GGTGGACCTG ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC 
ATCTATGGCT CCAGCGCCGG CTTCCACGCT GTGGATGTGG ACTCGGGGAA CAGCTACGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACGGACG GCATGGAGAT GCTGCTGTGC TATGAGGATG AGGGTGTCTA CGTCAACACG 
TACGGCCGGA TCATCAAGGA CGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCCGTG 
GAGACGGGCC ACCTGGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGGA ACGACAAGGT ATTTTTCGCC TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT 
TACTTCATGA CCTTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980163.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835931.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa226371
Clone ID Related Accession (Same CDS sequence)	XM_024980163.1
Accession Version	XM_024980163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCAGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835931.1
CDS	227..4075
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980163.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980163.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCAGCGA TGGAGACACA GACAGCGTCA GCACCATGGT GGTCCACGAC 
GTGGAAGAGA TAGCCGGGCC CCAGACCCCC TACGGGGGTG GCACCATGGT GGTCCAGCGA 
ACCCCCGAGG AGGAGCGCAG CCTGTTGCAT GCCGACAGCA ACGGCTACAC AAACCTGCCA 
GACGTGGTCC AGCCCAGCCA CTCGCCCACT GAGAGCAGCA AAGGTCAAAG CCCCCCCTTG 
AAGGATGGAG GCAGTGACTA CCAGTCTCGT GGGCTGGTAA AAGCCCCTGG CAAGAGCTCA 
TTCACGATGT TTGTGGACCT GGGGATCTAC CAGCCCGGAG GCAGTGGGGA CACCATTCCC 
ATTACAGCTC TGGTGGGTGG AGAGGGCAGC CGGCTCGATC AGCTACAGTA CGACGTGAGG 
AAAGGCTCTG TAGTCAACGT GAACCCCACC AACACCCGGG CCCACAGCGA AACCCCCGAG 
ATTCGGAAGT ACAAGAAGCG GTTCAATTCT GAGATCCTCT GTGCGGCCCT TTGGGGGGTC 
AACCTGCTGG TGGGCACGGA GAATGGGCTG ATGTTGCTGG ACCGCAGCGG GCAGGGCAAG 
GTGTATGGAC TCATCGGCCG GCGGCGCTTC CAGCAAATGG ACGTGCTGGA GGGACTCAAC 
TTGCTCATCA CCATCTCAGG GAAAAGGAAC AAACTGCGGG TGTATTACCT GTCCTGGCTC 
CGGAACAAGA TTCTGCACAA TGACCCGGAA GTGGAGAAGA AGCAAGGCTG GACCACTGTG 
GGGGACATGG AGGGCTGCGG GCACTACCGC GTGGTGAAAT ACGAACGCAT CAAGTTCCTA 
GTCATCGCCC TGAAGAACTC TGTGGAGGTG TATGCCTGGG CCCCCAAACC TTACCACAAA 
TTCATGGCTT TCAAGTCCTT TGCTGACCTC CCGCACCGCC CTCTGCTGGT GGACCTGACC 
GTAGAGGAGG GCCAGCGGCT CAAGGTCATC TATGGCTCCA GCGCCGGCTT CCACGCTGTG 
GATGTGGACT CGGGGAACAG CTACGACATC TACATCCCTG TACATATCCA GAGCCAGATC 
ACACCCCACG CCATCATCTT CCTCCCCAAC ACGGACGGCA TGGAGATGCT GCTGTGCTAT 
GAGGATGAGG GTGTCTACGT CAACACGTAC GGCCGGATCA TCAAGGACGT GGTGCTGCAG 
TGGGGAGAGA TGCCCACCTC TGTGGCCTAC ATCTGCTCCA ACCAGATCAT GGGCTGGGGA 
GAGAAAGCCA TTGAGATCCG CTCCGTGGAG ACGGGCCACC TGGATGGGGT CTTCATGCAC 
AAACGAGCCC AGAGGCTCAA GTTCCTGTGT GAGCGGAACG ACAAGGTATT TTTCGCCTCG 
GTCCGCTCCG GGGGCAGCAG CCAAGTTTAC TTCATGACCT TGAACCGTAA CTGCATCATG 
AACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980164.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835932.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OBa226372
Clone ID Related Accession (Same CDS sequence)	XM_024980164.1
Accession Version	XM_024980164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCGATGG AGACACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTG 
GAAGAGATAG CCGGGCCCCA GACCCCCTAC GGGGGTGGCA CCATGGTGGT CCAGCGAACC 
CCCGAGGAGG AGCGCAGCCT GTTGCATGCC GACAGCAACG GCTACACAAA CCTGCCAGAC 
GTGGTCCAGC CCAGCCACTC GCCCACTGAG AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG 
GATGGAGGCA GTGACTACCA GTCTCGTGGG CTGGTAAAAG CCCCTGGCAA GAGCTCATTC 
ACGATGTTTG TGGACCTGGG GATCTACCAG CCCGGAGGCA GTGGGGACAC CATTCCCATT 
ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG CTCGATCAGC TACAGTACGA CGTGAGGAAA 
GGCTCTGTAG TCAACGTGAA CCCCACCAAC ACCCGGGCCC ACAGCGAAAC CCCCGAGATT 
CGGAAGTACA AGAAGCGGTT CAATTCTGAG ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC 
CTGCTGGTGG GCACGGAGAA TGGGCTGATG TTGCTGGACC GCAGCGGGCA GGGCAAGGTG 
TATGGACTCA TCGGCCGGCG GCGCTTCCAG CAAATGGACG TGCTGGAGGG ACTCAACTTG 
CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG 
AACAAGATTC TGCACAATGA CCCGGAAGTG GAGAAGAAGC AAGGCTGGAC CACTGTGGGG 
GACATGGAGG GCTGCGGGCA CTACCGCGTG GTGAAATACG AACGCATCAA GTTCCTAGTC 
ATCGCCCTGA AGAACTCTGT GGAGGTGTAT GCCTGGGCCC CCAAACCTTA CCACAAATTC 
ATGGCTTTCA AGTCCTTTGC TGACCTCCCG CACCGCCCTC TGCTGGTGGA CCTGACCGTA 
GAGGAGGGCC AGCGGCTCAA GGTCATCTAT GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT 
GTGGACTCGG GGAACAGCTA CGACATCTAC ATCCCTGTAC ATATCCAGAG CCAGATCACA 
CCCCACGCCA TCATCTTCCT CCCCAACACG GACGGCATGG AGATGCTGCT GTGCTATGAG 
GATGAGGGTG TCTACGTCAA CACGTACGGC CGGATCATCA AGGACGTGGT GCTGCAGTGG 
GGAGAGATGC CCACCTCTGT GGCCTACATC TGCTCCAACC AGATCATGGG CTGGGGAGAG 
AAAGCCATTG AGATCCGCTC CGTGGAGACG GGCCACCTGG ATGGGGTCTT CATGCACAAA 
CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG CGGAACGACA AGGTATTTTT CGCCTCGGTC 
CGCTCCGGGG GCAGCAGCCA AGTTTACTTC ATGACCTTGA ACCGTAACTG CATCATGAAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835932.1
CDS	227..4072
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980164.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980164.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAAGCT ATAAGCGAGC CATTGGTGAG GATTTCGTCT TGCTGAAAGA GCGGACCCTG 
GACGAGGCCC CCCGGCCTCC CAAGAAGGCG ATGGACTACT CATCGTCCAG CGAGGAGGTG 
GAGAGCAGCG AGGACGAGGA GGAGGAGAGC AACGGCGAGC CGTCAGAGGG GAGCAGGGAC 
GCCCCTGGGG CCCGCGATGG AGACACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTG 
GAAGAGATAG CCGGGCCCCA GACCCCCTAC GGGGGTGGCA CCATGGTGGT CCAGCGAACC 
CCCGAGGAGG AGCGCAGCCT GTTGCATGCC GACAGCAACG GCTACACAAA CCTGCCAGAC 
GTGGTCCAGC CCAGCCACTC GCCCACTGAG AGCAGCAAAG GTCAAAGCCC CCCCTTGAAG 
GATGGAGGCA GTGACTACCA GTCTCGTGGG CTGGTAAAAG CCCCTGGCAA GAGCTCATTC 
ACGATGTTTG TGGACCTGGG GATCTACCAG CCCGGAGGCA GTGGGGACAC CATTCCCATT 
ACAGCTCTGG TGGGTGGAGA GGGCAGCCGG CTCGATCAGC TACAGTACGA CGTGAGGAAA 
GGCTCTGTAG TCAACGTGAA CCCCACCAAC ACCCGGGCCC ACAGCGAAAC CCCCGAGATT 
CGGAAGTACA AGAAGCGGTT CAATTCTGAG ATCCTCTGTG CGGCCCTTTG GGGGGTCAAC 
CTGCTGGTGG GCACGGAGAA TGGGCTGATG TTGCTGGACC GCAGCGGGCA GGGCAAGGTG 
TATGGACTCA TCGGCCGGCG GCGCTTCCAG CAAATGGACG TGCTGGAGGG ACTCAACTTG 
CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG 
AACAAGATTC TGCACAATGA CCCGGAAGTG GAGAAGAAGC AAGGCTGGAC CACTGTGGGG 
GACATGGAGG GCTGCGGGCA CTACCGCGTG GTGAAATACG AACGCATCAA GTTCCTAGTC 
ATCGCCCTGA AGAACTCTGT GGAGGTGTAT GCCTGGGCCC CCAAACCTTA CCACAAATTC 
ATGGCTTTCA AGTCCTTTGC TGACCTCCCG CACCGCCCTC TGCTGGTGGA CCTGACCGTA 
GAGGAGGGCC AGCGGCTCAA GGTCATCTAT GGCTCCAGCG CCGGCTTCCA CGCTGTGGAT 
GTGGACTCGG GGAACAGCTA CGACATCTAC ATCCCTGTAC ATATCCAGAG CCAGATCACA 
CCCCACGCCA TCATCTTCCT CCCCAACACG GACGGCATGG AGATGCTGCT GTGCTATGAG 
GATGAGGGTG TCTACGTCAA CACGTACGGC CGGATCATCA AGGACGTGGT GCTGCAGTGG 
GGAGAGATGC CCACCTCTGT GGCCTACATC TGCTCCAACC AGATCATGGG CTGGGGAGAG 
AAAGCCATTG AGATCCGCTC CGTGGAGACG GGCCACCTGG ATGGGGTCTT CATGCACAAA 
CGAGCCCAGA GGCTCAAGTT CCTGTGTGAG CGGAACGACA AGGTATTTTT CGCCTCGGTC 
CGCTCCGGGG GCAGCAGCCA AGTTTACTTC ATGACCTTGA ACCGTAACTG CATCATGAAC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980165.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835933.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X26, mRNA.

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CloneID	OBa226373
Clone ID Related Accession (Same CDS sequence)	XM_024980165.1
Accession Version	XM_024980165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CAGCGATGGA 
GACACAGACA GCGTCAGCAC CATGGTGGTC CACGACGTGG AAGAGATAGC CGGGCCCCAG 
ACCCCCTACG GGGGTGGCAC CATGGTGGTC CAGCGAACCC CCGAGGAGGA GCGCAGCCTG 
TTGCATGCCG ACAGCAACGG CTACACAAAC CTGCCAGACG TGGTCCAGCC CAGCCACTCG 
CCCACTGAGA GCAGCAAAGG TCAAAGCCCC CCCTTGAAGG ATGGAGGCAG TGACTACCAG 
TCTCGTGGGC TGGTAAAAGC CCCTGGCAAG AGCTCATTCA CGATGTTTGT GGACCTGGGG 
ATCTACCAGC CCGGAGGCAG TGGGGACACC ATTCCCATTA CAGCTCTGGT GGGTGGAGAG 
GGCAGCCGGC TCGATCAGCT ACAGTACGAC GTGAGGAAAG GCTCTGTAGT CAACGTGAAC 
CCCACCAACA CCCGGGCCCA CAGCGAAACC CCCGAGATTC GGAAGTACAA GAAGCGGTTC 
AATTCTGAGA TCCTCTGTGC GGCCCTTTGG GGGGTCAACC TGCTGGTGGG CACGGAGAAT 
GGGCTGATGT TGCTGGACCG CAGCGGGCAG GGCAAGGTGT ATGGACTCAT CGGCCGGCGG 
CGCTTCCAGC AAATGGACGT GCTGGAGGGA CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTGTA TTACCTGTCC TGGCTCCGGA ACAAGATTCT GCACAATGAC 
CCGGAAGTGG AGAAGAAGCA AGGCTGGACC ACTGTGGGGG ACATGGAGGG CTGCGGGCAC 
TACCGCGTGG TGAAATACGA ACGCATCAAG TTCCTAGTCA TCGCCCTGAA GAACTCTGTG 
GAGGTGTATG CCTGGGCCCC CAAACCTTAC CACAAATTCA TGGCTTTCAA GTCCTTTGCT 
GACCTCCCGC ACCGCCCTCT GCTGGTGGAC CTGACCGTAG AGGAGGGCCA GCGGCTCAAG 
GTCATCTATG GCTCCAGCGC CGGCTTCCAC GCTGTGGATG TGGACTCGGG GAACAGCTAC 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACGG ACGGCATGGA GATGCTGCTG TGCTATGAGG ATGAGGGTGT CTACGTCAAC 
ACGTACGGCC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATCATGGGC TGGGGAGAGA AAGCCATTGA GATCCGCTCC 
GTGGAGACGG GCCACCTGGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GGAACGACAA GGTATTTTTC GCCTCGGTCC GCTCCGGGGG CAGCAGCCAA 
GTTTACTTCA TGACCTTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835933.1
CDS	227..4051
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARSDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980165.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980165.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CAGCGATGGA 
GACACAGACA GCGTCAGCAC CATGGTGGTC CACGACGTGG AAGAGATAGC CGGGCCCCAG 
ACCCCCTACG GGGGTGGCAC CATGGTGGTC CAGCGAACCC CCGAGGAGGA GCGCAGCCTG 
TTGCATGCCG ACAGCAACGG CTACACAAAC CTGCCAGACG TGGTCCAGCC CAGCCACTCG 
CCCACTGAGA GCAGCAAAGG TCAAAGCCCC CCCTTGAAGG ATGGAGGCAG TGACTACCAG 
TCTCGTGGGC TGGTAAAAGC CCCTGGCAAG AGCTCATTCA CGATGTTTGT GGACCTGGGG 
ATCTACCAGC CCGGAGGCAG TGGGGACACC ATTCCCATTA CAGCTCTGGT GGGTGGAGAG 
GGCAGCCGGC TCGATCAGCT ACAGTACGAC GTGAGGAAAG GCTCTGTAGT CAACGTGAAC 
CCCACCAACA CCCGGGCCCA CAGCGAAACC CCCGAGATTC GGAAGTACAA GAAGCGGTTC 
AATTCTGAGA TCCTCTGTGC GGCCCTTTGG GGGGTCAACC TGCTGGTGGG CACGGAGAAT 
GGGCTGATGT TGCTGGACCG CAGCGGGCAG GGCAAGGTGT ATGGACTCAT CGGCCGGCGG 
CGCTTCCAGC AAATGGACGT GCTGGAGGGA CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTGTA TTACCTGTCC TGGCTCCGGA ACAAGATTCT GCACAATGAC 
CCGGAAGTGG AGAAGAAGCA AGGCTGGACC ACTGTGGGGG ACATGGAGGG CTGCGGGCAC 
TACCGCGTGG TGAAATACGA ACGCATCAAG TTCCTAGTCA TCGCCCTGAA GAACTCTGTG 
GAGGTGTATG CCTGGGCCCC CAAACCTTAC CACAAATTCA TGGCTTTCAA GTCCTTTGCT 
GACCTCCCGC ACCGCCCTCT GCTGGTGGAC CTGACCGTAG AGGAGGGCCA GCGGCTCAAG 
GTCATCTATG GCTCCAGCGC CGGCTTCCAC GCTGTGGATG TGGACTCGGG GAACAGCTAC 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACGG ACGGCATGGA GATGCTGCTG TGCTATGAGG ATGAGGGTGT CTACGTCAAC 
ACGTACGGCC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATCATGGGC TGGGGAGAGA AAGCCATTGA GATCCGCTCC 
GTGGAGACGG GCCACCTGGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GGAACGACAA GGTATTTTTC GCCTCGGTCC GCTCCGGGGG CAGCAGCCAA 
GTTTACTTCA TGACCTTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( XM_024980166.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835934.1 Bos taurus misshapen like kinase 1 (MINK1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa226374
Clone ID Related Accession (Same CDS sequence)	XM_024980166.1
Accession Version	XM_024980166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CGATGGAGAC 
ACAGACAGCG TCAGCACCAT GGTGGTCCAC GACGTGGAAG AGATAGCCGG GCCCCAGACC 
CCCTACGGGG GTGGCACCAT GGTGGTCCAG CGAACCCCCG AGGAGGAGCG CAGCCTGTTG 
CATGCCGACA GCAACGGCTA CACAAACCTG CCAGACGTGG TCCAGCCCAG CCACTCGCCC 
ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC TTGAAGGATG GAGGCAGTGA CTACCAGTCT 
CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC TCATTCACGA TGTTTGTGGA CCTGGGGATC 
TACCAGCCCG GAGGCAGTGG GGACACCATT CCCATTACAG CTCTGGTGGG TGGAGAGGGC 
AGCCGGCTCG ATCAGCTACA GTACGACGTG AGGAAAGGCT CTGTAGTCAA CGTGAACCCC 
ACCAACACCC GGGCCCACAG CGAAACCCCC GAGATTCGGA AGTACAAGAA GCGGTTCAAT 
TCTGAGATCC TCTGTGCGGC CCTTTGGGGG GTCAACCTGC TGGTGGGCAC GGAGAATGGG 
CTGATGTTGC TGGACCGCAG CGGGCAGGGC AAGGTGTATG GACTCATCGG CCGGCGGCGC 
TTCCAGCAAA TGGACGTGCT GGAGGGACTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTGTATTA CCTGTCCTGG CTCCGGAACA AGATTCTGCA CAATGACCCG 
GAAGTGGAGA AGAAGCAAGG CTGGACCACT GTGGGGGACA TGGAGGGCTG CGGGCACTAC 
CGCGTGGTGA AATACGAACG CATCAAGTTC CTAGTCATCG CCCTGAAGAA CTCTGTGGAG 
GTGTATGCCT GGGCCCCCAA ACCTTACCAC AAATTCATGG CTTTCAAGTC CTTTGCTGAC 
CTCCCGCACC GCCCTCTGCT GGTGGACCTG ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC 
ATCTATGGCT CCAGCGCCGG CTTCCACGCT GTGGATGTGG ACTCGGGGAA CAGCTACGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACGGACG GCATGGAGAT GCTGCTGTGC TATGAGGATG AGGGTGTCTA CGTCAACACG 
TACGGCCGGA TCATCAAGGA CGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCCGTG 
GAGACGGGCC ACCTGGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGGA ACGACAAGGT ATTTTTCGCC TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT 
TACTTCATGA CCTTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835934.1
CDS	227..4048
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_024980166.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980166.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGTAACCC CGACCTCAGG 
AGGAGCGACC CCGGCTGGGA GCGCTCAGAC AGCGTCCTCC CGGCCTCTCA TGGGCACCTC 
CCACAAGCCG GCTCGCTGGA GCGGAACCGG GTGGGAGCCT CCTCCAAACT GGACAACTCC 
CCTGTGCTCT CCCCCGGAAA CAAAACCAAG CCTGATGACC ACCGCTCGCG GCCAGGCCGG 
CCCGCAGATT TCGTCTTGCT GAAAGAGCGG ACCCTGGACG AGGCCCCCCG GCCTCCCAAG 
AAGGCGATGG ACTACTCATC GTCCAGCGAG GAGGTGGAGA GCAGCGAGGA CGAGGAGGAG 
GAGAGCAACG GCGAGCCGTC AGAGGGGAGC AGGGACGCCC CTGGGGCCCG CGATGGAGAC 
ACAGACAGCG TCAGCACCAT GGTGGTCCAC GACGTGGAAG AGATAGCCGG GCCCCAGACC 
CCCTACGGGG GTGGCACCAT GGTGGTCCAG CGAACCCCCG AGGAGGAGCG CAGCCTGTTG 
CATGCCGACA GCAACGGCTA CACAAACCTG CCAGACGTGG TCCAGCCCAG CCACTCGCCC 
ACTGAGAGCA GCAAAGGTCA AAGCCCCCCC TTGAAGGATG GAGGCAGTGA CTACCAGTCT 
CGTGGGCTGG TAAAAGCCCC TGGCAAGAGC TCATTCACGA TGTTTGTGGA CCTGGGGATC 
TACCAGCCCG GAGGCAGTGG GGACACCATT CCCATTACAG CTCTGGTGGG TGGAGAGGGC 
AGCCGGCTCG ATCAGCTACA GTACGACGTG AGGAAAGGCT CTGTAGTCAA CGTGAACCCC 
ACCAACACCC GGGCCCACAG CGAAACCCCC GAGATTCGGA AGTACAAGAA GCGGTTCAAT 
TCTGAGATCC TCTGTGCGGC CCTTTGGGGG GTCAACCTGC TGGTGGGCAC GGAGAATGGG 
CTGATGTTGC TGGACCGCAG CGGGCAGGGC AAGGTGTATG GACTCATCGG CCGGCGGCGC 
TTCCAGCAAA TGGACGTGCT GGAGGGACTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTGTATTA CCTGTCCTGG CTCCGGAACA AGATTCTGCA CAATGACCCG 
GAAGTGGAGA AGAAGCAAGG CTGGACCACT GTGGGGGACA TGGAGGGCTG CGGGCACTAC 
CGCGTGGTGA AATACGAACG CATCAAGTTC CTAGTCATCG CCCTGAAGAA CTCTGTGGAG 
GTGTATGCCT GGGCCCCCAA ACCTTACCAC AAATTCATGG CTTTCAAGTC CTTTGCTGAC 
CTCCCGCACC GCCCTCTGCT GGTGGACCTG ACCGTAGAGG AGGGCCAGCG GCTCAAGGTC 
ATCTATGGCT CCAGCGCCGG CTTCCACGCT GTGGATGTGG ACTCGGGGAA CAGCTACGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACGGACG GCATGGAGAT GCTGCTGTGC TATGAGGATG AGGGTGTCTA CGTCAACACG 
TACGGCCGGA TCATCAAGGA CGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT CATGGGCTGG GGAGAGAAAG CCATTGAGAT CCGCTCCGTG 
GAGACGGGCC ACCTGGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGGA ACGACAAGGT ATTTTTCGCC TCGGTCCGCT CCGGGGGCAG CAGCCAAGTT 
TACTTCATGA CCTTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MINK1 ( NM_001099376.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001092846.1 Bos taurus misshapen like kinase 1 (MINK1), mRNA.

Price & Availability↑

CloneID	OBa40630
Clone ID Related Accession (Same CDS sequence)	NM_001099376.1
Accession Version	NM_001099376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	misshapen-like kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146263.1. On or before Jul 3, 2007 this sequence version replaced XM_869465.2, XM_882582.2, XM_001254073.1, XM_001254086.1. ##Evidence-Data-START## Transcript exon combination :: BC146263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAGCTG 
CGGGTGTATT ACCTGTCCTG GCTCCGTAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092846.1
CDS	203..4135
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKAYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQILPGDRKPLYHYGRGINPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLQDQPTRNLAAFPASHDPDPAVPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGSPKPPGPPAPPPGPPNACSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDNSPVLSPGNKTKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDEEEESNGEPSEGSRDAPGARDGDTDSVSTMVVHDVEEIAGPQTPYGGGTMVVQRTPEEERSLLHADSNGYTNLPDVVQPSHSPTESSKGQSPPLKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGSRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_001099376.1
Organism	Bos taurus(cattle)
Definition	Bos taurus misshapen like kinase 1 (MINK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099376.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
CCTGCAGGCA TCTTCGAGCT GGTAGAGGTG GTCGGCAATG GCACCTACGG ACAGGTGTAC 
AAGGGTCGGC ATGTCAAGAC TGGGCAGCTG GCTGCCATCA AAGTCATGGA CGTCACGGAG 
GATGAGGAGG AAGAGATCAA ACAGGAGATC AACATGTTGA AAAAATACTC TCACCACCGC 
AACATCGCCA CCTACTATGG GGCTTTCATC AAGAAGAGCC CCCCAGGGAA CGACGACCAG 
CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCTGTAA CGGACTTGGT GAAGAACACG 
AAAGGGAATG CCCTGAAGGA GGACTGTATA GCCTACATCT GCAGGGAGAT TCTCCGGGGT 
CTGGCCCATC TCCACGCCCA CAAGGTGATC CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACAGAGA ATGCCGAGGT CAAGCTAGTG GATTTCGGGG TGAGCGCACA GCTGGACCGC 
ACCGTGGGCA GGCGGAACAC GTTCATCGGG ACCCCCTATT GGATGGCCCC CGAGGTCATC 
GCCTGTGATG AGAACCCTGA TGCCACCTAC GATTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA 
GCCCTCTTCC TCATCCCCCG GAACCCACCG CCCAGGCTCA AGTCCAAGAA ATGGTCTAAG 
AAGTTCATTG ACTTCATTGA CACGTGTCTC ATCAAGGCTT ACCTGAGCCG CCCACCCACC 
GAGCAGCTGC TCAAGTTCCC GTTCATCCGA GACCAGCCCA CCGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CAGATCCCGG AAGAAGCGAG GCGAGAAAGA GGAGACGGAA 
TACGAGTACA GCGGCAGCGA AGAGGAGGAC GACAGCCATG GAGAGGAAGG GGAGCCAAGC 
TCCATCATGA ACGTGCCGGG GGAGTCGACC CTCCGCCGGG AATTCCTCCG GCTCCAGCAG 
GAGAACAAGA GCAACTCGGA GGCTTTAAAG CAGCAGCAGC AGCAGCTGCA GCAGCAGCAA 
CAGCGAGACC CGGAGGCGCA CATCAAACAC CTCCTGCACC AGCGCCAGCG GCGCATCGAG 
GAGCAGAAGG AGGAGCGGCG GAGGGTTGAG GAGCAACAGC GACGGGAGCG GGAGCAGCGG 
AAGCTGCAGG AGAAGGAGCA GCAGCGGCGG CTGGAGGACA TGCAGGCCCT GCGGCGGGAG 
GAGGAGCGGC GGCAGGCCGA GCGCGAGCAG GAATACAAGC GGAAGCAGCT GGAGGAGCAA 
CGGCAGTCCG AGCGCCTGCA GAGGCAGCTG CAGCAGGAGC ACGCCTACCT CAAGTCCCTG 
CAGCAGCAGC AGCAACAGCA GCAGCTTCAG AAACAGCAGC AGATCCTGCC CGGGGACCGG 
AAGCCCCTGT ATCACTACGG TCGGGGCATC AACCCTGCCG ACAAGCCAGC CTGGGCCCGA 
GAGGTGGAAG AGAGAACAAG GATGAACAAA CAGCAGAACT CTCCCTTGGC CAAGAGCAAG 
CCAGGCAGCA CAGGGCCTGA GCCCCCCATC CCCCAGGCCT CCCCCGGGCC CCCAGGACCC 
CTTTCCCAAA CTCCTCCTAT GCAGAGGCCG GTGGAGCCCC AGGAGGGACC GCACAAGTCC 
CTGCAGGACC AACCCACCCG AAACCTGGCT GCCTTCCCCG CCTCCCACGA CCCCGACCCC 
GCCGTGCCCG CACCCACCGC CACGCCGAGT GCCCGAGGAG CCGTCATCCG GCAGAACTCA 
GACCCCACTT CCGAAGGGCC TGGCCCCAGC CCGAATCCCC CAGCCTGGGT CCGGCCTGAT 
AATGAGGCCC CTCCAAAGGT GCCTCAGAGG ACCTCATCAA TCGCCACTGC CCTTAACACC 
AGTGGGGCCG GAGGGTCCCG GCCAGCGCAG GCGGTCCGTG CTAGACCTCG CAGCAACTCC 
GCCTGGCAAA TCTATCTGCA AAGGCGGGCA GAGCGGGGCA GCCCCAAGCC TCCAGGGCCC 
CCCGCTCCGC CCCCCGGCCC GCCCAACGCC TGTAGTAACC CCGACCTCAG GAGGAGCGAC 
CCCGGCTGGG AGCGCTCAGA CAGCGTCCTC CCGGCCTCTC ATGGGCACCT CCCACAAGCC 
GGCTCGCTGG AGCGGAACCG GGTGGGAGCC TCCTCCAAAC TGGACAACTC CCCTGTGCTC 
TCCCCCGGAA ACAAAACCAA GCCTGATGAC CACCGCTCGC GGCCAGGCCG GCCCGCAGAT 
TTCGTCTTGC TGAAAGAGCG GACCCTGGAC GAGGCCCCCC GGCCTCCCAA GAAGGCGATG 
GACTACTCAT CGTCCAGCGA GGAGGTGGAG AGCAGCGAGG ACGAGGAGGA GGAGAGCAAC 
GGCGAGCCGT CAGAGGGGAG CAGGGACGCC CCTGGGGCCC GCGATGGAGA CACAGACAGC 
GTCAGCACCA TGGTGGTCCA CGACGTGGAA GAGATAGCCG GGCCCCAGAC CCCCTACGGG 
GGTGGCACCA TGGTGGTCCA GCGAACCCCC GAGGAGGAGC GCAGCCTGTT GCATGCCGAC 
AGCAACGGCT ACACAAACCT GCCAGACGTG GTCCAGCCCA GCCACTCGCC CACTGAGAGC 
AGCAAAGGTC AAAGCCCCCC CTTGAAGGAT GGAGGCAGTG ACTACCAGTC TCGTGGGCTG 
GTAAAAGCCC CTGGCAAGAG CTCATTCACG ATGTTTGTGG ACCTGGGGAT CTACCAGCCC 
GGAGGCAGTG GGGACACCAT TCCCATTACA GCTCTGGTGG GTGGAGAGGG CAGCCGGCTC 
GATCAGCTAC AGTACGACGT GAGGAAAGGC TCTGTAGTCA ACGTGAACCC CACCAACACC 
CGGGCCCACA GCGAAACCCC CGAGATTCGG AAGTACAAGA AGCGGTTCAA TTCTGAGATC 
CTCTGTGCGG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAATGG GCTGATGTTG 
CTGGACCGCA GCGGGCAGGG CAAGGTGTAT GGACTCATCG GCCGGCGGCG CTTCCAGCAA 
ATGGACGTGC TGGAGGGACT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAGCTG 
CGGGTGTATT ACCTGTCCTG GCTCCGTAAC AAGATTCTGC ACAATGACCC GGAAGTGGAG 
AAGAAGCAAG GCTGGACCAC TGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGCGTGGTG 
AAATACGAAC GCATCAAGTT CCTAGTCATC GCCCTGAAGA ACTCTGTGGA GGTGTATGCC 
TGGGCCCCCA AACCTTACCA CAAATTCATG GCTTTCAAGT CCTTTGCTGA CCTCCCGCAC 
CGCCCTCTGC TGGTGGACCT GACCGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC 
TCCAGCGCCG GCTTCCACGC TGTGGATGTG GACTCGGGGA ACAGCTACGA CATCTACATC 
CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACGGAC 
GGCATGGAGA TGCTGCTGTG CTATGAGGAT GAGGGTGTCT ACGTCAACAC GTACGGCCGG 
ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC 
TCCAACCAGA TCATGGGCTG GGGAGAGAAA GCCATTGAGA TCCGCTCCGT GGAGACGGGC 
CACCTGGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG 
AACGACAAGG TATTTTTCGC CTCGGTCCGC TCCGGGGGCA GCAGCCAAGT TTACTTCATG 
ACCTTGAACC GTAACTGCAT CATGAACTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MINK1
Entrez Gene ID	519558
Full Name	misshapen like kinase 1
Synonyms	MINK
General protein information	Preferred Names misshapen like kinase 1 Names misshapen-like kinase 1 GCK family kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Mink1	NP_001039429.1
Canis lupus familiaris (dog)	MINK1	XP_005619987.1
Homo sapiens (human)	MINK1	NP_722549.2
Bos taurus (cattle)	MINK1	NP_001092846.1
Rattus norvegicus (Norway rat)	Mink1	NP_001258065.1
Xenopus tropicalis (tropical clawed frog)	mink1	NP_001072739.1
	MINK1	XP_001117764.2
Pan troglodytes (chimpanzee)	MINK1	XP_511292.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MINK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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