COL20A1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015466100.1	XP_015321586.1	collagen alpha-1(XX) chain isoform X2
XM_025001114.1	XP_024856882.1	collagen alpha-1(XX) chain isoform X3
XM_015474240.1	XP_015329726.1	collagen alpha-1(XX) chain isoform X3
XM_025001113.1	XP_024856881.1	collagen alpha-1(XX) chain isoform X2
XM_015466101.1	XP_015321587.1	collagen alpha-1(XX) chain isoform X3
XM_015474238.2	XP_015329724.1	collagen alpha-1(XX) chain isoform X5
XM_015474238.1	XP_015329724.1	collagen alpha-1(XX) chain isoform X1
XM_015474239.1	XP_015329725.1	collagen alpha-1(XX) chain isoform X2
XM_015466099.1	XP_015321585.1	collagen alpha-1(XX) chain isoform X1
XM_025001112.1	XP_024856880.1	collagen alpha-1(XX) chain isoform X1
XM_025001115.1	XP_024856883.1	collagen alpha-1(XX) chain isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221968	XM_025001115.1 Latest version!	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa50056	XM_025001112.1 Latest version!	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa50057	XM_025001113.1 Latest version!	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa50058	XM_025001114.1 Latest version!	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa92383	XM_015474238.2 Latest version!	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92383	XM_015466099.1 Latest version!	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92384	XM_015466100.1 Latest version!	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92384	XM_015474239.1 Latest version!	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92385	XM_015474240.1 Latest version!	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92385	XM_015466101.1 Latest version!	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBa92383	XM_015474238.1	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

COL20A1 ( XM_025001115.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856883.1 Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa221968
Clone ID Related Accession (Same CDS sequence)	XM_025001115.1
Accession Version	XM_025001115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGAAGG AGGGAGAGTC CTGTCCTGCC 
TTCCCATCTG CCTGTGCATG TTCCTCACAG ACCCCTGGGC CCCCAGGCCC CCAAGGACCT 
CCGGGCCTCC CCGGGCGGAA CGGAGCCCCA GGAGAGCAAG GCTTCCCAGG GCCCAGGGGA 
GAGCCCGGGC CACCTGGACA GATGGGACCC GAGGGTCCTG GGGGTCAGCA GGGGTCACCG 
GGCACCCAGG GCCGCACCAT CCAGGGACCC GTGGGTCCGC CAGGGGTCAA AGGAGAGAAG 
GGGGACCACG GACACCCCGG CTTGCAGGGC CACCCCGGCC TCCAGGGCAC CCCCGGGAAG 
ATTGGCATCC AGGGACCGAA GGGAATGAGA GGCCTGGAGG GGACTGCTGG ACTGCCTGGC 
CCCCCAGGCC CTAGGGGCTT CCAAGGTACT TCGGGGGCTA GGGGCAGCAA CGGCGAGCGA 
GGACCCCCAG GGGCCGTGGG GCCCACAGGA CTGCCGGGGC CCAAGGGGGA GCGAGGAGAG 
AAGGGAGAGC CACAGTCCCT GGCCACCATC TACCAGCTCG TGGGCCAGGC TTGCGAGTCC 
GCCATCCAGA GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856883.1
CDS	95..3970
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGGAKSGDGVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQRRSPCCLK

Target ORF information:

RefSeq Version	XM_025001115.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001115.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGAAGG AGGGAGAGTC CTGTCCTGCC 
TTCCCATCTG CCTGTGCATG TTCCTCACAG ACCCCTGGGC CCCCAGGCCC CCAAGGACCT 
CCGGGCCTCC CCGGGCGGAA CGGAGCCCCA GGAGAGCAAG GCTTCCCAGG GCCCAGGGGA 
GAGCCCGGGC CACCTGGACA GATGGGACCC GAGGGTCCTG GGGGTCAGCA GGGGTCACCG 
GGCACCCAGG GCCGCACCAT CCAGGGACCC GTGGGTCCGC CAGGGGTCAA AGGAGAGAAG 
GGGGACCACG GACACCCCGG CTTGCAGGGC CACCCCGGCC TCCAGGGCAC CCCCGGGAAG 
ATTGGCATCC AGGGACCGAA GGGAATGAGA GGCCTGGAGG GGACTGCTGG ACTGCCTGGC 
CCCCCAGGCC CTAGGGGCTT CCAAGGTACT TCGGGGGCTA GGGGCAGCAA CGGCGAGCGA 
GGACCCCCAG GGGCCGTGGG GCCCACAGGA CTGCCGGGGC CCAAGGGGGA GCGAGGAGAG 
AAGGGAGAGC CACAGTCCCT GGCCACCATC TACCAGCTCG TGGGCCAGGC TTGCGAGTCC 
GCCATCCAGA GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_025001112.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856880.1 Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50056
Clone ID Related Accession (Same CDS sequence)	XM_025001112.1
Accession Version	XM_025001112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGAAGG AGGGAGAGTC CTGTCCTGCC 
TTCCCATCTG CCTGTGCATG TTCCTCACAG ACCCCTGGGC CCCCAGGCCC CCAAGGACCT 
CCGGGCCTCC CCGGGCGGAA CGGAGCCCCA GGAGAGCAAG GCTTCCCAGG GCCCAGGGGA 
GAGCCCGGGC CACCTGGACA GATGGGACCC GAGGGTCCTG GGGGTCAGCA GGGGTCACCG 
GGCACCCAGG GCCGCACCAT CCAGGGACCC GTGGGTCCGC CAGGGGTCAA AGGAGAGAAG 
GGGGACCACG GACACCCCGG CTTGCAGGGC CACCCCGGCC TCCAGGGCAC CCCCGGGAAG 
ATTGGCATCC AGGGACCGAA GGGAATGAGA GGCCTGGAGG GGACTGCTGG ACTGCCTGGC 
CCCCCAGGCC CTAGGGGCTT CCAAGGTACT TCGGGGGCTA GGGGCAGCAA CGGCGAGCGA 
GGACCCCCAG GGGCCGTGGG GCCCACAGGA CTGCCGGGGC CCAAGGGGGA GCGAGGAGAG 
AAGGGAGAGC CACAGTCCCT GGCCACCATC TACCAGCTCG TGGGCCAGGC TTGCGAGTCC 
GCCATCCAGA CGCACGTGTT GAAGCTCCAT GCCTGCACCC ACGAGAGCAC CCGGCCCCCC 
ATGCCCATCT TGGAAGCCCC CAGGGCCCTT GGCACAAGCA ACAAGGCCCG GCTCCCTGGA 
GAAGGGGGGC ATGGTGGCCC CCACCCTGGG GACAGAGGAC GGTCCCCCTG CTGCCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856880.1
CDS	95..4117
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGGAKSGDGVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_025001112.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001112.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGAAGG AGGGAGAGTC CTGTCCTGCC 
TTCCCATCTG CCTGTGCATG TTCCTCACAG ACCCCTGGGC CCCCAGGCCC CCAAGGACCT 
CCGGGCCTCC CCGGGCGGAA CGGAGCCCCA GGAGAGCAAG GCTTCCCAGG GCCCAGGGGA 
GAGCCCGGGC CACCTGGACA GATGGGACCC GAGGGTCCTG GGGGTCAGCA GGGGTCACCG 
GGCACCCAGG GCCGCACCAT CCAGGGACCC GTGGGTCCGC CAGGGGTCAA AGGAGAGAAG 
GGGGACCACG GACACCCCGG CTTGCAGGGC CACCCCGGCC TCCAGGGCAC CCCCGGGAAG 
ATTGGCATCC AGGGACCGAA GGGAATGAGA GGCCTGGAGG GGACTGCTGG ACTGCCTGGC 
CCCCCAGGCC CTAGGGGCTT CCAAGGTACT TCGGGGGCTA GGGGCAGCAA CGGCGAGCGA 
GGACCCCCAG GGGCCGTGGG GCCCACAGGA CTGCCGGGGC CCAAGGGGGA GCGAGGAGAG 
AAGGGAGAGC CACAGTCCCT GGCCACCATC TACCAGCTCG TGGGCCAGGC TTGCGAGTCC 
GCCATCCAGA CGCACGTGTT GAAGCTCCAT GCCTGCACCC ACGAGAGCAC CCGGCCCCCC 
ATGCCCATCT TGGAAGCCCC CAGGGCCCTT GGCACAAGCA ACAAGGCCCG GCTCCCTGGA 
GAAGGGGGGC ATGGTGGCCC CCACCCTGGG GACAGAGGAC GGTCCCCCTG CTGCCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_025001113.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856881.1 Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

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CloneID	OBa50057
Clone ID Related Accession (Same CDS sequence)	XM_025001113.1
Accession Version	XM_025001113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCC TGCCCGGCCC TCCACCTGGA TGGCTCCCTC 
CCAGGCTTTG ACCTGATGGC GGCCTTCGGG CTGGTGGAGA AAGAATATGC CTCCATCCGC 
GGCGTGGCCA TGGAGCCCTC GGCCTTTGGC CCCACCAGGA CGTTCACACT CTTCAAGGAC 
TCTCAGCTGA CCCGGCGGGC CAGCGACATC CACCTGGCCA CCCTGCCCCC GGAGCACACA 
GTCGTCTTCC TCCTGCGCCT GCTCCCTGAG ACTCCCCGAG AGGCCTTTGC ATTGTGGCAG 
GTGGCAAGCG AGGACTTCCA GCCCATCCTC GGGGTTCTGC TGGACGCCGG CAGGAAGTCG 
CTGACCTACT TCAACCGTGA CCCCAGGGCC ACCTTGCAGG AGGTCACCTT TGACCTGCCT 
GAAGTGAGGA GGATATTCTT TGGGAGTTTC CACAAGGTGC ATGTGGCCGT GGGCCGAGCC 
AGGGTCAGGC TCTACGTGGA CTGCCGGAAG GTGGCCGAGA GGCCCATCGG GGAGGCCGGC 
GGCCTGCCTG CTGCCGGCTT CGTCATGCTG GGCAGGTTGG CCAAAGCCCG GGGCCCCCGG 
AGCAGCTCCG CCTCGTTCCA GCTCCAGGTG CTACAGCTCG TGTGCAATGA CTCGTGGGCA 
GAGGAGGACA GGTGCTGTGA GCTCCCAGCA TCGAAGGAGG GAGAGTCCTG TCCTGCCTTC 
CCATCTGCCT GTGCATGTTC CTCACAGACC CCTGGGCCCC CAGGCCCCCA AGGACCTCCG 
GGCCTCCCCG GGCGGAACGG AGCCCCAGGA GAGCAAGGCT TCCCAGGGCC CAGGGGAGAG 
CCCGGGCCAC CTGGACAGAT GGGACCCGAG GGTCCTGGGG GTCAGCAGGG GTCACCGGGC 
ACCCAGGGCC GCACCATCCA GGGACCCGTG GGTCCGCCAG GGGTCAAAGG AGAGAAGGGG 
GACCACGGAC ACCCCGGCTT GCAGGGCCAC CCCGGCCTCC AGGGCACCCC CGGGAAGATT 
GGCATCCAGG GACCGAAGGG AATGAGAGGC CTGGAGGGGA CTGCTGGACT GCCTGGCCCC 
CCAGGCCCTA GGGGCTTCCA AGGTACTTCG GGGGCTAGGG GCAGCAACGG CGAGCGAGGA 
CCCCCAGGGG CCGTGGGGCC CACAGGACTG CCGGGGCCCA AGGGGGAGCG AGGAGAGAAG 
GGAGAGCCAC AGTCCCTGGC CACCATCTAC CAGCTCGTGG GCCAGGCTTG CGAGTCCGCC 
ATCCAGACGC ACGTGTTGAA GCTCCATGCC TGCACCCACG AGAGCACCCG GCCCCCCATG 
CCCATCTTGG AAGCCCCCAG GGCCCTTGGC ACAAGCAACA AGGCCCGGCT CCCTGGAGAA 
GGGGGGCATG GTGGCCCCCA CCCTGGGGAC AGAGGACGGT CCCCCTGCTG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856881.1
CDS	95..4114
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGGAKSGDGVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_025001113.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001113.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCC TGCCCGGCCC TCCACCTGGA TGGCTCCCTC 
CCAGGCTTTG ACCTGATGGC GGCCTTCGGG CTGGTGGAGA AAGAATATGC CTCCATCCGC 
GGCGTGGCCA TGGAGCCCTC GGCCTTTGGC CCCACCAGGA CGTTCACACT CTTCAAGGAC 
TCTCAGCTGA CCCGGCGGGC CAGCGACATC CACCTGGCCA CCCTGCCCCC GGAGCACACA 
GTCGTCTTCC TCCTGCGCCT GCTCCCTGAG ACTCCCCGAG AGGCCTTTGC ATTGTGGCAG 
GTGGCAAGCG AGGACTTCCA GCCCATCCTC GGGGTTCTGC TGGACGCCGG CAGGAAGTCG 
CTGACCTACT TCAACCGTGA CCCCAGGGCC ACCTTGCAGG AGGTCACCTT TGACCTGCCT 
GAAGTGAGGA GGATATTCTT TGGGAGTTTC CACAAGGTGC ATGTGGCCGT GGGCCGAGCC 
AGGGTCAGGC TCTACGTGGA CTGCCGGAAG GTGGCCGAGA GGCCCATCGG GGAGGCCGGC 
GGCCTGCCTG CTGCCGGCTT CGTCATGCTG GGCAGGTTGG CCAAAGCCCG GGGCCCCCGG 
AGCAGCTCCG CCTCGTTCCA GCTCCAGGTG CTACAGCTCG TGTGCAATGA CTCGTGGGCA 
GAGGAGGACA GGTGCTGTGA GCTCCCAGCA TCGAAGGAGG GAGAGTCCTG TCCTGCCTTC 
CCATCTGCCT GTGCATGTTC CTCACAGACC CCTGGGCCCC CAGGCCCCCA AGGACCTCCG 
GGCCTCCCCG GGCGGAACGG AGCCCCAGGA GAGCAAGGCT TCCCAGGGCC CAGGGGAGAG 
CCCGGGCCAC CTGGACAGAT GGGACCCGAG GGTCCTGGGG GTCAGCAGGG GTCACCGGGC 
ACCCAGGGCC GCACCATCCA GGGACCCGTG GGTCCGCCAG GGGTCAAAGG AGAGAAGGGG 
GACCACGGAC ACCCCGGCTT GCAGGGCCAC CCCGGCCTCC AGGGCACCCC CGGGAAGATT 
GGCATCCAGG GACCGAAGGG AATGAGAGGC CTGGAGGGGA CTGCTGGACT GCCTGGCCCC 
CCAGGCCCTA GGGGCTTCCA AGGTACTTCG GGGGCTAGGG GCAGCAACGG CGAGCGAGGA 
CCCCCAGGGG CCGTGGGGCC CACAGGACTG CCGGGGCCCA AGGGGGAGCG AGGAGAGAAG 
GGAGAGCCAC AGTCCCTGGC CACCATCTAC CAGCTCGTGG GCCAGGCTTG CGAGTCCGCC 
ATCCAGACGC ACGTGTTGAA GCTCCATGCC TGCACCCACG AGAGCACCCG GCCCCCCATG 
CCCATCTTGG AAGCCCCCAG GGCCCTTGGC ACAAGCAACA AGGCCCGGCT CCCTGGAGAA 
GGGGGGCATG GTGGCCCCCA CCCTGGGGAC AGAGGACGGT CCCCCTGCTG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_025001114.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856882.1 Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

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CloneID	OBa50058
Clone ID Related Accession (Same CDS sequence)	XM_025001114.1
Accession Version	XM_025001114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGGAGG GAGAGTCCTG TCCTGCCTTC 
CCATCTGCCT GTGCATGTTC CTCACAGACC CCTGGGCCCC CAGGCCCCCA AGGACCTCCG 
GGCCTCCCCG GGCGGAACGG AGCCCCAGGA GAGCAAGGCT TCCCAGGGCC CAGGGGAGAG 
CCCGGGCCAC CTGGACAGAT GGGACCCGAG GGTCCTGGGG GTCAGCAGGG GTCACCGGGC 
ACCCAGGGCC GCACCATCCA GGGACCCGTG GGTCCGCCAG GGGTCAAAGG AGAGAAGGGG 
GACCACGGAC ACCCCGGCTT GCAGGGCCAC CCCGGCCTCC AGGGCACCCC CGGGAAGATT 
GGCATCCAGG GACCGAAGGG AATGAGAGGC CTGGAGGGGA CTGCTGGACT GCCTGGCCCC 
CCAGGCCCTA GGGGCTTCCA AGGTACTTCG GGGGCTAGGG GCAGCAACGG CGAGCGAGGA 
CCCCCAGGGG CCGTGGGGCC CACAGGACTG CCGGGGCCCA AGGGGGAGCG AGGAGAGAAG 
GGAGAGCCAC AGTCCCTGGC CACCATCTAC CAGCTCGTGG GCCAGGCTTG CGAGTCCGCC 
ATCCAGACGC ACGTGTTGAA GCTCCATGCC TGCACCCACG AGAGCACCCG GCCCCCCATG 
CCCATCTTGG AAGCCCCCAG GGCCCTTGGC ACAAGCAACA AGGCCCGGCT CCCTGGAGAA 
GGGGGGCATG GTGGCCCCCA CCCTGGGGAC AGAGGACGGT CCCCCTGCTG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856882.1
CDS	95..4114
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGGAKSGDGVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_025001114.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001114.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGTGGAGCCA AGTCAGGGGA CGGAGTACCT GGTGAGGAGC AGCACCCACC CAGGGGCCCC 
ACAGGGGAGG GCCCTGCTCA GCCCACCCCA GACCTGGAGG GGCCGAACCA CGCCAGGACC 
TCAGCTGGAG GGAGAGGGAA GCCAGGCAGA CCCCAGTTCC GCTGCACACC CCCCATGCCT 
GTGGACATGA TCTTCCTGGT GGATGGGTCC TGGAGTATAG GCCACAGTCA CTTCCAGCAG 
GTCAAAGACT TCCTGGCCAG CGTCATCGAG CCCTTTGAAA TTGGGCCGGG CAAAGTCCAA 
GTAGGTCTGA CTCAGTACAG TGGAGCCCCC CAGACCGAGT GGGACCTGAA TGCCTTTGGC 
ACCAAGGAGG AGGTGTTGAA TGCTGTGCAC AACCTCCACT ACAGAGGAGG AAACACATTC 
ACAGGCCTGG CCCTGACCCA CGTGCTGGAG CAGAACCTGA AGCCCAGAGC AGGTCTCCGT 
CCAGAGGCGG CCAAGCTGGT GATTCTGGTG ACAGATGGCA AGTCCCAGGA TGATGCCCAC 
ACTGCTGGCC ACGTGCTCAA GGGTCTGGGT GTCGACATCT TTGCTGTGGG CGTGAAGAAT 
GCCGACGAAA CCGAGTTGAG GCTCCTGGCA TCCCAGCCGC TGGACATCAC CGTCCACAAT 
GTGCAGGACT TCCCCCAGCT CAGCACACTC TCCGGCCTAC TCAGCCGGCT CATCTGCCAG 
AAGGTCCAGG GCAGGAGCCC GCGTGGGAGC CCAGCTACAC CGGCCCTGGA TCCCCCTCCT 
GCTCCCACAA GGCTGGTCCT GACCCAAGTG ACCTCCTCCA GCGTCCTCTT GTCCTGGAGC 
CCGGCCCCAC AGCCGCCCCT CAAGTACCTG ATTGTGTGGA GGCCTTCCAA GGGCGGCACC 
CCCAGGGAGG TGGTGGTGGA AGGGCCCACC TCGTCAGCAC AGCTGCACAA CCTGACCTCC 
AGCACCGAGT ACCTGGTCTC TGTGTTCCCC CTCTACAAGG CTGGCGTCGG CGAGGGCCTG 
CAGGGCCTGG CGACCACAGC ACCCCTGCCC CCACCCCAGG CGCTGACCCT GGCTGCAGTG 
ACACCCAAAA CTGTCCGCCT CACCTGGCAG CCCTCGGCAG AGGCCACCCA GTACCTGGTG 
CGGTGGTCAC CTGCCTCCCC CAAGGGCGAG GAGGAGAGGA GAGAGGTGCG GGTGGGGCGG 
CCGGAGGTGC TGCTGGATGG CCTGGCCCCG GGCAGGGACT ATGACGTGTG GGTGCAGAGC 
CTTCGAGGGG CAGAGATCAG TGAGGCCCAG GGCATCCGTG CCAGGACCCC CGCCCTGACC 
ACCCCCAGAC ACCTGAGCTT CTCAGACGTG AGTCATGATT CAGCCCGCGT GTCCTGGGAG 
GGCACCGCAA GACCTGTGCG CCTGTTCAGG GTCAGCTACA TCTCGGGCAA GGGAGGCCAC 
TCGGGACAGA CAGAGGTTCC TGGGAACGCC ACCTCAGCCA TCCTGGGGCC CCTCTCCTCC 
TCTACCACCT ACATGGTCCG CGTCACCTGT CTCTACCCTG GGGGCAGCTC CTCTACCCTG 
ACCAGCCGCC TGACCACACG GAAAGTCCCC AGCCCGAGCC AGCTGTCGGT GACAGAACTG 
CCTGGGGACG AAGTCCGGCT CGAGTGGGCG GCCGCGGCGG TGTCCGGCGT GCTTGTCTAC 
CAGATCACGT GGATGCCCCT GGGAGAGGGG AAGGCCCGTG AGATTTCTGT CCCGGGGAAC 
CTGGGCACGG CCATCCTGCC TGGCCTGGGG AGGCACTCAG AGTATGAGAT CACCATCCTG 
GCCTACTACC GGGACGGGGC CCGCAGTGAC CCTGTGTCCC TCCGTTACAC CCCCGTCAGC 
CGGAGCCCAC CCTCCAACCT GGCCTTGGCC TCAGAGTCGC CTGACAGCCT GCGGGTCAGC 
TGGACGCCCC CGAGCAGTCA CGTGCTCCAC TACCGGCTCT CCTACGCGCT GGCCTCAGGC 
TCTGGACCCG AGAAGTCGAT CTCCATTCCA GGACCCGGGA GCCACGTGAC GATCCCTGAC 
CTGCTGGCGG CTACCAAGTA CCGGGTCCTG GTCTCAGCCA TCTATGGAGC AGGGGAGAGC 
GTGGCGGTGT CTGCCACAGG CCGGACGGCA GCCTGCCCGG CCCTCCACCT GGATGGCTCC 
CTCCCAGGCT TTGACCTGAT GGCGGCCTTC GGGCTGGTGG AGAAAGAATA TGCCTCCATC 
CGCGGCGTGG CCATGGAGCC CTCGGCCTTT GGCCCCACCA GGACGTTCAC ACTCTTCAAG 
GACTCTCAGC TGACCCGGCG GGCCAGCGAC ATCCACCTGG CCACCCTGCC CCCGGAGCAC 
ACAGTCGTCT TCCTCCTGCG CCTGCTCCCT GAGACTCCCC GAGAGGCCTT TGCATTGTGG 
CAGGTGGCAA GCGAGGACTT CCAGCCCATC CTCGGGGTTC TGCTGGACGC CGGCAGGAAG 
TCGCTGACCT ACTTCAACCG TGACCCCAGG GCCACCTTGC AGGAGGTCAC CTTTGACCTG 
CCTGAAGTGA GGAGGATATT CTTTGGGAGT TTCCACAAGG TGCATGTGGC CGTGGGCCGA 
GCCAGGGTCA GGCTCTACGT GGACTGCCGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCC 
GGCGGCCTGC CTGCTGCCGG CTTCGTCATG CTGGGCAGGT TGGCCAAAGC CCGGGGCCCC 
CGGAGCAGCT CCGCCTCGTT CCAGCTCCAG GTGCTACAGC TCGTGTGCAA TGACTCGTGG 
GCAGAGGAGG ACAGGTGCTG TGAGCTCCCA GCATCGGAGG GAGAGTCCTG TCCTGCCTTC 
CCATCTGCCT GTGCATGTTC CTCACAGACC CCTGGGCCCC CAGGCCCCCA AGGACCTCCG 
GGCCTCCCCG GGCGGAACGG AGCCCCAGGA GAGCAAGGCT TCCCAGGGCC CAGGGGAGAG 
CCCGGGCCAC CTGGACAGAT GGGACCCGAG GGTCCTGGGG GTCAGCAGGG GTCACCGGGC 
ACCCAGGGCC GCACCATCCA GGGACCCGTG GGTCCGCCAG GGGTCAAAGG AGAGAAGGGG 
GACCACGGAC ACCCCGGCTT GCAGGGCCAC CCCGGCCTCC AGGGCACCCC CGGGAAGATT 
GGCATCCAGG GACCGAAGGG AATGAGAGGC CTGGAGGGGA CTGCTGGACT GCCTGGCCCC 
CCAGGCCCTA GGGGCTTCCA AGGTACTTCG GGGGCTAGGG GCAGCAACGG CGAGCGAGGA 
CCCCCAGGGG CCGTGGGGCC CACAGGACTG CCGGGGCCCA AGGGGGAGCG AGGAGAGAAG 
GGAGAGCCAC AGTCCCTGGC CACCATCTAC CAGCTCGTGG GCCAGGCTTG CGAGTCCGCC 
ATCCAGACGC ACGTGTTGAA GCTCCATGCC TGCACCCACG AGAGCACCCG GCCCCCCATG 
CCCATCTTGG AAGCCCCCAG GGCCCTTGGC ACAAGCAACA AGGCCCGGCT CCCTGGAGAA 
GGGGGGCATG GTGGCCCCCA CCCTGGGGAC AGAGGACGGT CCCCCTGCTG CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015474238.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329724.1 Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92383
Clone ID Related Accession (Same CDS sequence)	XM_015474238.2 , XM_015474238.1 , XM_015466099.1
Accession Version	XM_015474238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329724.1
CDS	95..3952
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVRVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015474238.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474238.2

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015474238.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329724.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

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CloneID	OBa92383
Clone ID Related Accession (Same CDS sequence)	XM_015474238.2 , XM_015474238.1 , XM_015466099.1
Accession Version	XM_015474238.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329724.1
CDS	79..3936
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVRVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015474238.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474238.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015466099.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321585.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92383
Clone ID Related Accession (Same CDS sequence)	XM_015474238.2 , XM_015474238.1 , XM_015466099.1
Accession Version	XM_015466099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321585.1
CDS	79..3936
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVRVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTAACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015466099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466099.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCAGCCTG CCCGGCCCTC 
CACCTGGATG GCTCCCTCCC AGGCTTTGAC CTGATGGCGG CCTTCGGGCT GGTGGAGAAA 
GAATATGCCT CCATCCGCGG CGTGGCCATG GAGCCCTCGG CCTTTGGCCC CACCAGGACG 
TTCACACTCT TCAAGGACTC TCAGCTGACC CGGCGGGCCA GCGACATCCA CCTGGCCACC 
CTGCCCCCGG AGCACACAGT CGTCTTCCTC CTGCGCCTGC TCCCTGAGAC TCCCCGAGAG 
GCCTTTGCAT TGTGGCAGGT GGCAAGCGAG GACTTCCAGC CCATCCTCGG GGTTCTGCTG 
GACGCCGGCA GGAAGTCGCT GACCTACTTC AACCGTGACC CCAGGGCCAC CTTGCAGGAG 
GTCACCTTTG ACCTGCCTGA AGTGAGGAGG ATATTCTTTG GGAGTTTCCA CAAGGTGCAT 
GTGGCCGTGG GCCGAGCCAG GGTCAGGCTC TACGTGGACT GCCGGAAGGT GGCCGAGAGG 
CCCATCGGGG AGGCCGGCGG CCTGCCTGCT GCCGGCTTCG TCATGCTGGG CAGGTTGGCC 
AAAGCCCGGG GCCCCCGGAG CAGCTCCGCC TCGTTCCAGC TCCAGGTGCT ACAGCTCGTG 
TGCAATGACT CGTGGGCAGA GGAGGACAGG TGCTGTGAGC TCCCAGCATC GAAGGAGGGA 
GAGTCCTGTC CTGCCTTCCC ATCTGCCTGT GCATGTTCCT CACAGACCCC TGGGCCCCCA 
GGCCCCCAAG GACCTCCGGG CCTCCCCGGG CGGAACGGAG CCCCAGGAGA GCAAGGCTTC 
CCAGGGCCCA GGGGAGAGCC CGGGCCACCT GGACAGATGG GACCCGAGGG TCCTGGGGGT 
CAGCAGGGGT CACCGGGCAC CCAGGGCCGC ACCATCCAGG GACCCGTGGG TCCGCCAGGG 
GTCAAAGGAG AGAAGGGGGA CCACGGACAC CCCGGCTTGC AGGGCCACCC CGGCCTCCAG 
GGCACCCCCG GGAAGATTGG CATCCAGGGA CCGAAGGGAA TGAGAGGCCT GGAGGGGACT 
GCTGGACTGC CTGGCCCCCC AGGCCCTAGG GGCTTCCAAG GTACTTCGGG GGCTAGGGGC 
AGCAACGGCG AGCGAGGACC CCCAGGGGCC GTGGGGCCCA CAGGACTGCC GGGGCCCAAG 
GGGGAGCGAG GAGAGAAGGG AGAGCCACAG TCCCTGGCCA CCATCTACCA GCTCGTGGGC 
CAGGCTTGCG AGTCCGCCAT CCAGACGCAC GTGTTGAAGC TCCATGCCTG CACCCACGAG 
AGCACCCGGC CCCCCATGCC CATCTTGGAA GCCCCCAGGG CCCTTGGCAC AAGCAACAAG 
GCCCGGCTCC CTGGAGAAGG GGGGCATGGT GGCCCCCACC CTGGGGACAG AGGACGGTCC 
CCCTGCTGCC TCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015466100.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321586.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92384
Clone ID Related Accession (Same CDS sequence)	XM_015466100.1 , XM_015474239.1
Accession Version	XM_015466100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCCTGCCC GGCCCTCCAC 
CTGGATGGCT CCCTCCCAGG CTTTGACCTG ATGGCGGCCT TCGGGCTGGT GGAGAAAGAA 
TATGCCTCCA TCCGCGGCGT GGCCATGGAG CCCTCGGCCT TTGGCCCCAC CAGGACGTTC 
ACACTCTTCA AGGACTCTCA GCTGACCCGG CGGGCCAGCG ACATCCACCT GGCCACCCTG 
CCCCCGGAGC ACACAGTCGT CTTCCTCCTG CGCCTGCTCC CTGAGACTCC CCGAGAGGCC 
TTTGCATTGT GGCAGGTGGC AAGCGAGGAC TTCCAGCCCA TCCTCGGGGT TCTGCTGGAC 
GCCGGCAGGA AGTCGCTGAC CTACTTCAAC CGTGACCCCA GGGCCACCTT GCAGGAGGTC 
ACCTTTGACC TGCCTGAAGT GAGGAGGATA TTCTTTGGGA GTTTCCACAA GGTGCATGTG 
GCCGTGGGCC GAGCCAGGGT CAGGCTCTAC GTGGACTGCC GGAAGGTGGC CGAGAGGCCC 
ATCGGGGAGG CCGGCGGCCT GCCTGCTGCC GGCTTCGTCA TGCTGGGCAG GTTGGCCAAA 
GCCCGGGGCC CCCGGAGCAG CTCCGCCTCG TTCCAGCTCC AGGTGCTACA GCTCGTGTGC 
AATGACTCGT GGGCAGAGGA GGACAGGTGC TGTGAGCTCC CAGCATCGAA GGAGGGAGAG 
TCCTGTCCTG CCTTCCCATC TGCCTGTGCA TGTTCCTCAC AGACCCCTGG GCCCCCAGGC 
CCCCAAGGAC CTCCGGGCCT CCCCGGGCGG AACGGAGCCC CAGGAGAGCA AGGCTTCCCA 
GGGCCCAGGG GAGAGCCCGG GCCACCTGGA CAGATGGGAC CCGAGGGTCC TGGGGGTCAG 
CAGGGGTCAC CGGGCACCCA GGGCCGCACC ATCCAGGGAC CCGTGGGTCC GCCAGGGGTC 
AAAGGAGAGA AGGGGGACCA CGGACACCCC GGCTTGCAGG GCCACCCCGG CCTCCAGGGC 
ACCCCCGGGA AGATTGGCAT CCAGGGACCG AAGGGAATGA GAGGCCTGGA GGGGACTGCT 
GGACTGCCTG GCCCCCCAGG CCCTAGGGGC TTCCAAGGTA CTTCGGGGGC TAGGGGCAGC 
AACGGCGAGC GAGGACCCCC AGGGGCCGTG GGGCCCACAG GACTGCCGGG GCCCAAGGGG 
GAGCGAGGAG AGAAGGGAGA GCCACAGTCC CTGGCCACCA TCTACCAGCT CGTGGGCCAG 
GCTTGCGAGT CCGCCATCCA GACGCACGTG TTGAAGCTCC ATGCCTGCAC CCACGAGAGC 
ACCCGGCCCC CCATGCCCAT CTTGGAAGCC CCCAGGGCCC TTGGCACAAG CAACAAGGCC 
CGGCTCCCTG GAGAAGGGGG GCATGGTGGC CCCCACCCTG GGGACAGAGG ACGGTCCCCC 
TGCTGCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321586.1
CDS	79..3933
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVRVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015466100.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466100.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCCTGCCC GGCCCTCCAC 
CTGGATGGCT CCCTCCCAGG CTTTGACCTG ATGGCGGCCT TCGGGCTGGT GGAGAAAGAA 
TATGCCTCCA TCCGCGGCGT GGCCATGGAG CCCTCGGCCT TTGGCCCCAC CAGGACGTTC 
ACACTCTTCA AGGACTCTCA GCTGACCCGG CGGGCCAGCG ACATCCACCT GGCCACCCTG 
CCCCCGGAGC ACACAGTCGT CTTCCTCCTG CGCCTGCTCC CTGAGACTCC CCGAGAGGCC 
TTTGCATTGT GGCAGGTGGC AAGCGAGGAC TTCCAGCCCA TCCTCGGGGT TCTGCTGGAC 
GCCGGCAGGA AGTCGCTGAC CTACTTCAAC CGTGACCCCA GGGCCACCTT GCAGGAGGTC 
ACCTTTGACC TGCCTGAAGT GAGGAGGATA TTCTTTGGGA GTTTCCACAA GGTGCATGTG 
GCCGTGGGCC GAGCCAGGGT CAGGCTCTAC GTGGACTGCC GGAAGGTGGC CGAGAGGCCC 
ATCGGGGAGG CCGGCGGCCT GCCTGCTGCC GGCTTCGTCA TGCTGGGCAG GTTGGCCAAA 
GCCCGGGGCC CCCGGAGCAG CTCCGCCTCG TTCCAGCTCC AGGTGCTACA GCTCGTGTGC 
AATGACTCGT GGGCAGAGGA GGACAGGTGC TGTGAGCTCC CAGCATCGAA GGAGGGAGAG 
TCCTGTCCTG CCTTCCCATC TGCCTGTGCA TGTTCCTCAC AGACCCCTGG GCCCCCAGGC 
CCCCAAGGAC CTCCGGGCCT CCCCGGGCGG AACGGAGCCC CAGGAGAGCA AGGCTTCCCA 
GGGCCCAGGG GAGAGCCCGG GCCACCTGGA CAGATGGGAC CCGAGGGTCC TGGGGGTCAG 
CAGGGGTCAC CGGGCACCCA GGGCCGCACC ATCCAGGGAC CCGTGGGTCC GCCAGGGGTC 
AAAGGAGAGA AGGGGGACCA CGGACACCCC GGCTTGCAGG GCCACCCCGG CCTCCAGGGC 
ACCCCCGGGA AGATTGGCAT CCAGGGACCG AAGGGAATGA GAGGCCTGGA GGGGACTGCT 
GGACTGCCTG GCCCCCCAGG CCCTAGGGGC TTCCAAGGTA CTTCGGGGGC TAGGGGCAGC 
AACGGCGAGC GAGGACCCCC AGGGGCCGTG GGGCCCACAG GACTGCCGGG GCCCAAGGGG 
GAGCGAGGAG AGAAGGGAGA GCCACAGTCC CTGGCCACCA TCTACCAGCT CGTGGGCCAG 
GCTTGCGAGT CCGCCATCCA GACGCACGTG TTGAAGCTCC ATGCCTGCAC CCACGAGAGC 
ACCCGGCCCC CCATGCCCAT CTTGGAAGCC CCCAGGGCCC TTGGCACAAG CAACAAGGCC 
CGGCTCCCTG GAGAAGGGGG GCATGGTGGC CCCCACCCTG GGGACAGAGG ACGGTCCCCC 
TGCTGCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015474239.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329725.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

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CloneID	OBa92384
Clone ID Related Accession (Same CDS sequence)	XM_015466100.1 , XM_015474239.1
Accession Version	XM_015474239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCCTGCCC GGCCCTCCAC 
CTGGATGGCT CCCTCCCAGG CTTTGACCTG ATGGCGGCCT TCGGGCTGGT GGAGAAAGAA 
TATGCCTCCA TCCGCGGCGT GGCCATGGAG CCCTCGGCCT TTGGCCCCAC CAGGACGTTC 
ACACTCTTCA AGGACTCTCA GCTGACCCGG CGGGCCAGCG ACATCCACCT GGCCACCCTG 
CCCCCGGAGC ACACAGTCGT CTTCCTCCTG CGCCTGCTCC CTGAGACTCC CCGAGAGGCC 
TTTGCATTGT GGCAGGTGGC AAGCGAGGAC TTCCAGCCCA TCCTCGGGGT TCTGCTGGAC 
GCCGGCAGGA AGTCGCTGAC CTACTTCAAC CGTGACCCCA GGGCCACCTT GCAGGAGGTC 
ACCTTTGACC TGCCTGAAGT GAGGAGGATA TTCTTTGGGA GTTTCCACAA GGTGCATGTG 
GCCGTGGGCC GAGCCAGGGT CAGGCTCTAC GTGGACTGCC GGAAGGTGGC CGAGAGGCCC 
ATCGGGGAGG CCGGCGGCCT GCCTGCTGCC GGCTTCGTCA TGCTGGGCAG GTTGGCCAAA 
GCCCGGGGCC CCCGGAGCAG CTCCGCCTCG TTCCAGCTCC AGGTGCTACA GCTCGTGTGC 
AATGACTCGT GGGCAGAGGA GGACAGGTGC TGTGAGCTCC CAGCATCGAA GGAGGGAGAG 
TCCTGTCCTG CCTTCCCATC TGCCTGTGCA TGTTCCTCAC AGACCCCTGG GCCCCCAGGC 
CCCCAAGGAC CTCCGGGCCT CCCCGGGCGG AACGGAGCCC CAGGAGAGCA AGGCTTCCCA 
GGGCCCAGGG GAGAGCCCGG GCCACCTGGA CAGATGGGAC CCGAGGGTCC TGGGGGTCAG 
CAGGGGTCAC CGGGCACCCA GGGCCGCACC ATCCAGGGAC CCGTGGGTCC GCCAGGGGTC 
AAAGGAGAGA AGGGGGACCA CGGACACCCC GGCTTGCAGG GCCACCCCGG CCTCCAGGGC 
ACCCCCGGGA AGATTGGCAT CCAGGGACCG AAGGGAATGA GAGGCCTGGA GGGGACTGCT 
GGACTGCCTG GCCCCCCAGG CCCTAGGGGC TTCCAAGGTA CTTCGGGGGC TAGGGGCAGC 
AACGGCGAGC GAGGACCCCC AGGGGCCGTG GGGCCCACAG GACTGCCGGG GCCCAAGGGG 
GAGCGAGGAG AGAAGGGAGA GCCACAGTCC CTGGCCACCA TCTACCAGCT CGTGGGCCAG 
GCTTGCGAGT CCGCCATCCA GACGCACGTG TTGAAGCTCC ATGCCTGCAC CCACGAGAGC 
ACCCGGCCCC CCATGCCCAT CTTGGAAGCC CCCAGGGCCC TTGGCACAAG CAACAAGGCC 
CGGCTCCCTG GAGAAGGGGG GCATGGTGGC CCCCACCCTG GGGACAGAGG ACGGTCCCCC 
TGCTGCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329725.1
CDS	79..3933
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVRVPGEEQHPPRGPTGEGPAQPTPDLEGPNHARTSAGGRGKPGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015474239.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474239.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGAGAG TACCTGGTGA GGAGCAGCAC 
CCACCCAGGG GCCCCACAGG GGAGGGCCCT GCTCAGCCCA CCCCAGACCT GGAGGGGCCG 
AACCACGCCA GGACCTCAGC TGGAGGGAGA GGGAAGCCAG GCAGACCCCA GTTCCGCTGC 
ACACCCCCCA TGCCTGTGGA CATGATCTTC CTGGTGGATG GGTCCTGGAG TATAGGCCAC 
AGTCACTTCC AGCAGGTCAA AGACTTCCTG GCCAGCGTCA TCGAGCCCTT TGAAATTGGG 
CCGGGCAAAG TCCAAGTAGG TCTGACTCAG TACAGTGGAG CCCCCCAGAC CGAGTGGGAC 
CTGAATGCCT TTGGCACCAA GGAGGAGGTG TTGAATGCTG TGCACAACCT CCACTACAGA 
GGAGGAAACA CATTCACAGG CCTGGCCCTG ACCCACGTGC TGGAGCAGAA CCTGAAGCCC 
AGAGCAGGTC TCCGTCCAGA GGCGGCCAAG CTGGTGATTC TGGTGACAGA TGGCAAGTCC 
CAGGATGATG CCCACACTGC TGGCCACGTG CTCAAGGGTC TGGGTGTCGA CATCTTTGCT 
GTGGGCGTGA AGAATGCCGA CGAAACCGAG TTGAGGCTCC TGGCATCCCA GCCGCTGGAC 
ATCACCGTCC ACAATGTGCA GGACTTCCCC CAGCTCAGCA CACTCTCCGG CCTACTCAGC 
CGGCTCATCT GCCAGAAGGT CCAGGGCAGG AGCCCGCGTG GGAGCCCAGC TACACCGGCC 
CTGGATCCCC CTCCTGCTCC CACAAGGCTG GTCCTGACCC AAGTGACCTC CTCCAGCGTC 
CTCTTGTCCT GGAGCCCGGC CCCACAGCCG CCCCTCAAGT ACCTGATTGT GTGGAGGCCT 
TCCAAGGGCG GCACCCCCAG GGAGGTGGTG GTGGAAGGGC CCACCTCGTC AGCACAGCTG 
CACAACCTGA CCTCCAGCAC CGAGTACCTG GTCTCTGTGT TCCCCCTCTA CAAGGCTGGC 
GTCGGCGAGG GCCTGCAGGG CCTGGCGACC ACAGCACCCC TGCCCCCACC CCAGGCGCTG 
ACCCTGGCTG CAGTGACACC CAAAACTGTC CGCCTCACCT GGCAGCCCTC GGCAGAGGCC 
ACCCAGTACC TGGTGCGGTG GTCACCTGCC TCCCCCAAGG GCGAGGAGGA GAGGAGAGAG 
GTGCGGGTGG GGCGGCCGGA GGTGCTGCTG GATGGCCTGG CCCCGGGCAG GGACTATGAC 
GTGTGGGTGC AGAGCCTTCG AGGGGCAGAG ATCAGTGAGG CCCAGGGCAT CCGTGCCAGG 
ACCCCCGCCC TGACCACCCC CAGACACCTG AGCTTCTCAG ACGTGAGTCA TGATTCAGCC 
CGCGTGTCCT GGGAGGGCAC CGCAAGACCT GTGCGCCTGT TCAGGGTCAG CTACATCTCG 
GGCAAGGGAG GCCACTCGGG ACAGACAGAG GTTCCTGGGA ACGCCACCTC AGCCATCCTG 
GGGCCCCTCT CCTCCTCTAC CACCTACATG GTCCGCGTCA CCTGTCTCTA CCCTGGGGGC 
AGCTCCTCTA CCCTGACCAG CCGCCTGACC ACACGGAAAG TCCCCAGCCC GAGCCAGCTG 
TCGGTGACAG AACTGCCTGG GGACGAAGTC CGGCTCGAGT GGGCGGCCGC GGCGGTGTCC 
GGCGTGCTTG TCTACCAGAT CACGTGGATG CCCCTGGGAG AGGGGAAGGC CCGTGAGATT 
TCTGTCCCGG GGAACCTGGG CACGGCCATC CTGCCTGGCC TGGGGAGGCA CTCAGAGTAT 
GAGATCACCA TCCTGGCCTA CTACCGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGT 
TACACCCCCG TCAGCCGGAG CCCACCCTCC AACCTGGCCT TGGCCTCAGA GTCGCCTGAC 
AGCCTGCGGG TCAGCTGGAC GCCCCCGAGC AGTCACGTGC TCCACTACCG GCTCTCCTAC 
GCGCTGGCCT CAGGCTCTGG ACCCGAGAAG TCGATCTCCA TTCCAGGACC CGGGAGCCAC 
GTGACGATCC CTGACCTGCT GGCGGCTACC AAGTACCGGG TCCTGGTCTC AGCCATCTAT 
GGAGCAGGGG AGAGCGTGGC GGTGTCTGCC ACAGGCCGGA CGGCCTGCCC GGCCCTCCAC 
CTGGATGGCT CCCTCCCAGG CTTTGACCTG ATGGCGGCCT TCGGGCTGGT GGAGAAAGAA 
TATGCCTCCA TCCGCGGCGT GGCCATGGAG CCCTCGGCCT TTGGCCCCAC CAGGACGTTC 
ACACTCTTCA AGGACTCTCA GCTGACCCGG CGGGCCAGCG ACATCCACCT GGCCACCCTG 
CCCCCGGAGC ACACAGTCGT CTTCCTCCTG CGCCTGCTCC CTGAGACTCC CCGAGAGGCC 
TTTGCATTGT GGCAGGTGGC AAGCGAGGAC TTCCAGCCCA TCCTCGGGGT TCTGCTGGAC 
GCCGGCAGGA AGTCGCTGAC CTACTTCAAC CGTGACCCCA GGGCCACCTT GCAGGAGGTC 
ACCTTTGACC TGCCTGAAGT GAGGAGGATA TTCTTTGGGA GTTTCCACAA GGTGCATGTG 
GCCGTGGGCC GAGCCAGGGT CAGGCTCTAC GTGGACTGCC GGAAGGTGGC CGAGAGGCCC 
ATCGGGGAGG CCGGCGGCCT GCCTGCTGCC GGCTTCGTCA TGCTGGGCAG GTTGGCCAAA 
GCCCGGGGCC CCCGGAGCAG CTCCGCCTCG TTCCAGCTCC AGGTGCTACA GCTCGTGTGC 
AATGACTCGT GGGCAGAGGA GGACAGGTGC TGTGAGCTCC CAGCATCGAA GGAGGGAGAG 
TCCTGTCCTG CCTTCCCATC TGCCTGTGCA TGTTCCTCAC AGACCCCTGG GCCCCCAGGC 
CCCCAAGGAC CTCCGGGCCT CCCCGGGCGG AACGGAGCCC CAGGAGAGCA AGGCTTCCCA 
GGGCCCAGGG GAGAGCCCGG GCCACCTGGA CAGATGGGAC CCGAGGGTCC TGGGGGTCAG 
CAGGGGTCAC CGGGCACCCA GGGCCGCACC ATCCAGGGAC CCGTGGGTCC GCCAGGGGTC 
AAAGGAGAGA AGGGGGACCA CGGACACCCC GGCTTGCAGG GCCACCCCGG CCTCCAGGGC 
ACCCCCGGGA AGATTGGCAT CCAGGGACCG AAGGGAATGA GAGGCCTGGA GGGGACTGCT 
GGACTGCCTG GCCCCCCAGG CCCTAGGGGC TTCCAAGGTA CTTCGGGGGC TAGGGGCAGC 
AACGGCGAGC GAGGACCCCC AGGGGCCGTG GGGCCCACAG GACTGCCGGG GCCCAAGGGG 
GAGCGAGGAG AGAAGGGAGA GCCACAGTCC CTGGCCACCA TCTACCAGCT CGTGGGCCAG 
GCTTGCGAGT CCGCCATCCA GACGCACGTG TTGAAGCTCC ATGCCTGCAC CCACGAGAGC 
ACCCGGCCCC CCATGCCCAT CTTGGAAGCC CCCAGGGCCC TTGGCACAAG CAACAAGGCC 
CGGCTCCCTG GAGAAGGGGG GCATGGTGGC CCCCACCCTG GGGACAGAGG ACGGTCCCCC 
TGCTGCCTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015474240.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329726.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa92385
Clone ID Related Accession (Same CDS sequence)	XM_015474240.1 , XM_015466101.1
Accession Version	XM_015474240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGCAGACCCC AGTTCCGCTG CACACCCCCC ATGCCTGTGG ACATGATCTT CCTGGTGGAT 
GGGTCCTGGA GTATAGGCCA CAGTCACTTC CAGCAGGTCA AAGACTTCCT GGCCAGCGTC 
ATCGAGCCCT TTGAAATTGG GCCGGGCAAA GTCCAAGTAG GTCTGACTCA GTACAGTGGA 
GCCCCCCAGA CCGAGTGGGA CCTGAATGCC TTTGGCACCA AGGAGGAGGT GTTGAATGCT 
GTGCACAACC TCCACTACAG AGGAGGAAAC ACATTCACAG GCCTGGCCCT GACCCACGTG 
CTGGAGCAGA ACCTGAAGCC CAGAGCAGGT CTCCGTCCAG AGGCGGCCAA GCTGGTGATT 
CTGGTGACAG ATGGCAAGTC CCAGGATGAT GCCCACACTG CTGGCCACGT GCTCAAGGGT 
CTGGGTGTCG ACATCTTTGC TGTGGGCGTG AAGAATGCCG ACGAAACCGA GTTGAGGCTC 
CTGGCATCCC AGCCGCTGGA CATCACCGTC CACAATGTGC AGGACTTCCC CCAGCTCAGC 
ACACTCTCCG GCCTACTCAG CCGGCTCATC TGCCAGAAGG TCCAGGGCAG GAGCCCGCGT 
GGGAGCCCAG CTACACCGGC CCTGGATCCC CCTCCTGCTC CCACAAGGCT GGTCCTGACC 
CAAGTGACCT CCTCCAGCGT CCTCTTGTCC TGGAGCCCGG CCCCACAGCC GCCCCTCAAG 
TACCTGATTG TGTGGAGGCC TTCCAAGGGC GGCACCCCCA GGGAGGTGGT GGTGGAAGGG 
CCCACCTCGT CAGCACAGCT GCACAACCTG ACCTCCAGCA CCGAGTACCT GGTCTCTGTG 
TTCCCCCTCT ACAAGGCTGG CGTCGGCGAG GGCCTGCAGG GCCTGGCGAC CACAGCACCC 
CTGCCCCCAC CCCAGGCGCT GACCCTGGCT GCAGTGACAC CCAAAACTGT CCGCCTCACC 
TGGCAGCCCT CGGCAGAGGC CACCCAGTAC CTGGTGCGGT GGTCACCTGC CTCCCCCAAG 
GGCGAGGAGG AGAGGAGAGA GGTGCGGGTG GGGCGGCCGG AGGTGCTGCT GGATGGCCTG 
GCCCCGGGCA GGGACTATGA CGTGTGGGTG CAGAGCCTTC GAGGGGCAGA GATCAGTGAG 
GCCCAGGGCA TCCGTGCCAG GACCCCCGCC CTGACCACCC CCAGACACCT GAGCTTCTCA 
GACGTGAGTC ATGATTCAGC CCGCGTGTCC TGGGAGGGCA CCGCAAGACC TGTGCGCCTG 
TTCAGGGTCA GCTACATCTC GGGCAAGGGA GGCCACTCGG GACAGACAGA GGTTCCTGGG 
AACGCCACCT CAGCCATCCT GGGGCCCCTC TCCTCCTCTA CCACCTACAT GGTCCGCGTC 
ACCTGTCTCT ACCCTGGGGG CAGCTCCTCT ACCCTGACCA GCCGCCTGAC CACACGGAAA 
GTCCCCAGCC CGAGCCAGCT GTCGGTGACA GAACTGCCTG GGGACGAAGT CCGGCTCGAG 
TGGGCGGCCG CGGCGGTGTC CGGCGTGCTT GTCTACCAGA TCACGTGGAT GCCCCTGGGA 
GAGGGGAAGG CCCGTGAGAT TTCTGTCCCG GGGAACCTGG GCACGGCCAT CCTGCCTGGC 
CTGGGGAGGC ACTCAGAGTA TGAGATCACC ATCCTGGCCT ACTACCGGGA CGGGGCCCGC 
AGTGACCCTG TGTCCCTCCG TTACACCCCC GTCAGCCGGA GCCCACCCTC CAACCTGGCC 
TTGGCCTCAG AGTCGCCTGA CAGCCTGCGG GTCAGCTGGA CGCCCCCGAG CAGTCACGTG 
CTCCACTACC GGCTCTCCTA CGCGCTGGCC TCAGGCTCTG GACCCGAGAA GTCGATCTCC 
ATTCCAGGAC CCGGGAGCCA CGTGACGATC CCTGACCTGC TGGCGGCTAC CAAGTACCGG 
GTCCTGGTCT CAGCCATCTA TGGAGCAGGG GAGAGCGTGG CGGTGTCTGC CACAGGCCGG 
ACGGCCTGCC CGGCCCTCCA CCTGGATGGC TCCCTCCCAG GCTTTGACCT GATGGCGGCC 
TTCGGGCTGG TGGAGAAAGA ATATGCCTCC ATCCGCGGCG TGGCCATGGA GCCCTCGGCC 
TTTGGCCCCA CCAGGACGTT CACACTCTTC AAGGACTCTC AGCTGACCCG GCGGGCCAGC 
GACATCCACC TGGCCACCCT GCCCCCGGAG CACACAGTCG TCTTCCTCCT GCGCCTGCTC 
CCTGAGACTC CCCGAGAGGC CTTTGCATTG TGGCAGGTGG CAAGCGAGGA CTTCCAGCCC 
ATCCTCGGGG TTCTGCTGGA CGCCGGCAGG AAGTCGCTGA CCTACTTCAA CCGTGACCCC 
AGGGCCACCT TGCAGGAGGT CACCTTTGAC CTGCCTGAAG TGAGGAGGAT ATTCTTTGGG 
AGTTTCCACA AGGTGCATGT GGCCGTGGGC CGAGCCAGGG TCAGGCTCTA CGTGGACTGC 
CGGAAGGTGG CCGAGAGGCC CATCGGGGAG GCCGGCGGCC TGCCTGCTGC CGGCTTCGTC 
ATGCTGGGCA GGTTGGCCAA AGCCCGGGGC CCCCGGAGCA GCTCCGCCTC GTTCCAGCTC 
CAGGTGCTAC AGCTCGTGTG CAATGACTCG TGGGCAGAGG AGGACAGGTG CTGTGAGCTC 
CCAGCATCGA AGGAGGGAGA GTCCTGTCCT GCCTTCCCAT CTGCCTGTGC ATGTTCCTCA 
CAGACCCCTG GGCCCCCAGG CCCCCAAGGA CCTCCGGGCC TCCCCGGGCG GAACGGAGCC 
CCAGGAGAGC AAGGCTTCCC AGGGCCCAGG GGAGAGCCCG GGCCACCTGG ACAGATGGGA 
CCCGAGGGTC CTGGGGGTCA GCAGGGGTCA CCGGGCACCC AGGGCCGCAC CATCCAGGGA 
CCCGTGGGTC CGCCAGGGGT CAAAGGAGAG AAGGGGGACC ACGGACACCC CGGCTTGCAG 
GGCCACCCCG GCCTCCAGGG CACCCCCGGG AAGATTGGCA TCCAGGGACC GAAGGGAATG 
AGAGGCCTGG AGGGGACTGC TGGACTGCCT GGCCCCCCAG GCCCTAGGGG CTTCCAAGGT 
ACTTCGGGGG CTAGGGGCAG CAACGGCGAG CGAGGACCCC CAGGGGCCGT GGGGCCCACA 
GGACTGCCGG GGCCCAAGGG GGAGCGAGGA GAGAAGGGAG AGCCACAGTC CCTGGCCACC 
ATCTACCAGC TCGTGGGCCA GGCTTGCGAG TCCGCCATCC AGACGCACGT GTTGAAGCTC 
CATGCCTGCA CCCACGAGAG CACCCGGCCC CCCATGCCCA TCTTGGAAGC CCCCAGGGCC 
CTTGGCACAA GCAACAAGGC CCGGCTCCCT GGAGAAGGGG GGCATGGTGG CCCCCACCCT 
GGGGACAGAG GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329726.1
CDS	79..3954
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015474240.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474240.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGCAGACCCC AGTTCCGCTG CACACCCCCC ATGCCTGTGG ACATGATCTT CCTGGTGGAT 
GGGTCCTGGA GTATAGGCCA CAGTCACTTC CAGCAGGTCA AAGACTTCCT GGCCAGCGTC 
ATCGAGCCCT TTGAAATTGG GCCGGGCAAA GTCCAAGTAG GTCTGACTCA GTACAGTGGA 
GCCCCCCAGA CCGAGTGGGA CCTGAATGCC TTTGGCACCA AGGAGGAGGT GTTGAATGCT 
GTGCACAACC TCCACTACAG AGGAGGAAAC ACATTCACAG GCCTGGCCCT GACCCACGTG 
CTGGAGCAGA ACCTGAAGCC CAGAGCAGGT CTCCGTCCAG AGGCGGCCAA GCTGGTGATT 
CTGGTGACAG ATGGCAAGTC CCAGGATGAT GCCCACACTG CTGGCCACGT GCTCAAGGGT 
CTGGGTGTCG ACATCTTTGC TGTGGGCGTG AAGAATGCCG ACGAAACCGA GTTGAGGCTC 
CTGGCATCCC AGCCGCTGGA CATCACCGTC CACAATGTGC AGGACTTCCC CCAGCTCAGC 
ACACTCTCCG GCCTACTCAG CCGGCTCATC TGCCAGAAGG TCCAGGGCAG GAGCCCGCGT 
GGGAGCCCAG CTACACCGGC CCTGGATCCC CCTCCTGCTC CCACAAGGCT GGTCCTGACC 
CAAGTGACCT CCTCCAGCGT CCTCTTGTCC TGGAGCCCGG CCCCACAGCC GCCCCTCAAG 
TACCTGATTG TGTGGAGGCC TTCCAAGGGC GGCACCCCCA GGGAGGTGGT GGTGGAAGGG 
CCCACCTCGT CAGCACAGCT GCACAACCTG ACCTCCAGCA CCGAGTACCT GGTCTCTGTG 
TTCCCCCTCT ACAAGGCTGG CGTCGGCGAG GGCCTGCAGG GCCTGGCGAC CACAGCACCC 
CTGCCCCCAC CCCAGGCGCT GACCCTGGCT GCAGTGACAC CCAAAACTGT CCGCCTCACC 
TGGCAGCCCT CGGCAGAGGC CACCCAGTAC CTGGTGCGGT GGTCACCTGC CTCCCCCAAG 
GGCGAGGAGG AGAGGAGAGA GGTGCGGGTG GGGCGGCCGG AGGTGCTGCT GGATGGCCTG 
GCCCCGGGCA GGGACTATGA CGTGTGGGTG CAGAGCCTTC GAGGGGCAGA GATCAGTGAG 
GCCCAGGGCA TCCGTGCCAG GACCCCCGCC CTGACCACCC CCAGACACCT GAGCTTCTCA 
GACGTGAGTC ATGATTCAGC CCGCGTGTCC TGGGAGGGCA CCGCAAGACC TGTGCGCCTG 
TTCAGGGTCA GCTACATCTC GGGCAAGGGA GGCCACTCGG GACAGACAGA GGTTCCTGGG 
AACGCCACCT CAGCCATCCT GGGGCCCCTC TCCTCCTCTA CCACCTACAT GGTCCGCGTC 
ACCTGTCTCT ACCCTGGGGG CAGCTCCTCT ACCCTGACCA GCCGCCTGAC CACACGGAAA 
GTCCCCAGCC CGAGCCAGCT GTCGGTGACA GAACTGCCTG GGGACGAAGT CCGGCTCGAG 
TGGGCGGCCG CGGCGGTGTC CGGCGTGCTT GTCTACCAGA TCACGTGGAT GCCCCTGGGA 
GAGGGGAAGG CCCGTGAGAT TTCTGTCCCG GGGAACCTGG GCACGGCCAT CCTGCCTGGC 
CTGGGGAGGC ACTCAGAGTA TGAGATCACC ATCCTGGCCT ACTACCGGGA CGGGGCCCGC 
AGTGACCCTG TGTCCCTCCG TTACACCCCC GTCAGCCGGA GCCCACCCTC CAACCTGGCC 
TTGGCCTCAG AGTCGCCTGA CAGCCTGCGG GTCAGCTGGA CGCCCCCGAG CAGTCACGTG 
CTCCACTACC GGCTCTCCTA CGCGCTGGCC TCAGGCTCTG GACCCGAGAA GTCGATCTCC 
ATTCCAGGAC CCGGGAGCCA CGTGACGATC CCTGACCTGC TGGCGGCTAC CAAGTACCGG 
GTCCTGGTCT CAGCCATCTA TGGAGCAGGG GAGAGCGTGG CGGTGTCTGC CACAGGCCGG 
ACGGCCTGCC CGGCCCTCCA CCTGGATGGC TCCCTCCCAG GCTTTGACCT GATGGCGGCC 
TTCGGGCTGG TGGAGAAAGA ATATGCCTCC ATCCGCGGCG TGGCCATGGA GCCCTCGGCC 
TTTGGCCCCA CCAGGACGTT CACACTCTTC AAGGACTCTC AGCTGACCCG GCGGGCCAGC 
GACATCCACC TGGCCACCCT GCCCCCGGAG CACACAGTCG TCTTCCTCCT GCGCCTGCTC 
CCTGAGACTC CCCGAGAGGC CTTTGCATTG TGGCAGGTGG CAAGCGAGGA CTTCCAGCCC 
ATCCTCGGGG TTCTGCTGGA CGCCGGCAGG AAGTCGCTGA CCTACTTCAA CCGTGACCCC 
AGGGCCACCT TGCAGGAGGT CACCTTTGAC CTGCCTGAAG TGAGGAGGAT ATTCTTTGGG 
AGTTTCCACA AGGTGCATGT GGCCGTGGGC CGAGCCAGGG TCAGGCTCTA CGTGGACTGC 
CGGAAGGTGG CCGAGAGGCC CATCGGGGAG GCCGGCGGCC TGCCTGCTGC CGGCTTCGTC 
ATGCTGGGCA GGTTGGCCAA AGCCCGGGGC CCCCGGAGCA GCTCCGCCTC GTTCCAGCTC 
CAGGTGCTAC AGCTCGTGTG CAATGACTCG TGGGCAGAGG AGGACAGGTG CTGTGAGCTC 
CCAGCATCGA AGGAGGGAGA GTCCTGTCCT GCCTTCCCAT CTGCCTGTGC ATGTTCCTCA 
CAGACCCCTG GGCCCCCAGG CCCCCAAGGA CCTCCGGGCC TCCCCGGGCG GAACGGAGCC 
CCAGGAGAGC AAGGCTTCCC AGGGCCCAGG GGAGAGCCCG GGCCACCTGG ACAGATGGGA 
CCCGAGGGTC CTGGGGGTCA GCAGGGGTCA CCGGGCACCC AGGGCCGCAC CATCCAGGGA 
CCCGTGGGTC CGCCAGGGGT CAAAGGAGAG AAGGGGGACC ACGGACACCC CGGCTTGCAG 
GGCCACCCCG GCCTCCAGGG CACCCCCGGG AAGATTGGCA TCCAGGGACC GAAGGGAATG 
AGAGGCCTGG AGGGGACTGC TGGACTGCCT GGCCCCCCAG GCCCTAGGGG CTTCCAAGGT 
ACTTCGGGGG CTAGGGGCAG CAACGGCGAG CGAGGACCCC CAGGGGCCGT GGGGCCCACA 
GGACTGCCGG GGCCCAAGGG GGAGCGAGGA GAGAAGGGAG AGCCACAGTC CCTGGCCACC 
ATCTACCAGC TCGTGGGCCA GGCTTGCGAG TCCGCCATCC AGACGCACGT GTTGAAGCTC 
CATGCCTGCA CCCACGAGAG CACCCGGCCC CCCATGCCCA TCTTGGAAGC CCCCAGGGCC 
CTTGGCACAA GCAACAAGGC CCGGCTCCCT GGAGAAGGGG GGCATGGTGG CCCCCACCCT 
GGGGACAGAG GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_015466101.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321587.1 Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa92385
Clone ID Related Accession (Same CDS sequence)	XM_015474240.1 , XM_015466101.1
Accession Version	XM_015466101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGCAGACCCC AGTTCCGCTG CACACCCCCC ATGCCTGTGG ACATGATCTT CCTGGTGGAT 
GGGTCCTGGA GTATAGGCCA CAGTCACTTC CAGCAGGTCA AAGACTTCCT GGCCAGCGTC 
ATCGAGCCCT TTGAAATTGG GCCGGGCAAA GTCCAAGTAG GTCTGACTCA GTACAGTGGA 
GCCCCCCAGA CCGAGTGGGA CCTGAATGCC TTTGGCACCA AGGAGGAGGT GTTGAATGCT 
GTGCACAACC TCCACTACAG AGGAGGAAAC ACATTCACAG GCCTGGCCCT GACCCACGTG 
CTGGAGCAGA ACCTGAAGCC CAGAGCAGGT CTCCGTCCAG AGGCGGCCAA GCTGGTGATT 
CTGGTGACAG ATGGCAAGTC CCAGGATGAT GCCCACACTG CTGGCCACGT GCTCAAGGGT 
CTGGGTGTCG ACATCTTTGC TGTGGGCGTG AAGAATGCCG ACGAAACCGA GTTGAGGCTC 
CTGGCATCCC AGCCGCTGGA CATCACCGTC CACAATGTGC AGGACTTCCC CCAGCTCAGC 
ACACTCTCCG GCCTACTCAG CCGGCTCATC TGCCAGAAGG TCCAGGGCAG GAGCCCGCGT 
GGGAGCCCAG CTACACCGGC CCTGGATCCC CCTCCTGCTC CCACAAGGCT GGTCCTGACC 
CAAGTGACCT CCTCCAGCGT CCTCTTGTCC TGGAGCCCGG CCCCACAGCC GCCCCTCAAG 
TACCTGATTG TGTGGAGGCC TTCCAAGGGC GGCACCCCCA GGGAGGTGGT GGTGGAAGGG 
CCCACCTCGT CAGCACAGCT GCACAACCTG ACCTCCAGCA CCGAGTACCT GGTCTCTGTG 
TTCCCCCTCT ACAAGGCTGG CGTCGGCGAG GGCCTGCAGG GCCTGGCGAC CACAGCACCC 
CTGCCCCCAC CCCAGGCGCT GACCCTGGCT GCAGTGACAC CCAAAACTGT CCGCCTCACC 
TGGCAGCCCT CGGCAGAGGC CACCCAGTAC CTGGTGCGGT GGTCACCTGC CTCCCCCAAG 
GGCGAGGAGG AGAGGAGAGA GGTGCGGGTG GGGCGGCCGG AGGTGCTGCT GGATGGCCTG 
GCCCCGGGCA GGGACTATGA CGTGTGGGTG CAGAGCCTTC GAGGGGCAGA GATCAGTGAG 
GCCCAGGGCA TCCGTGCCAG GACCCCCGCC CTGACCACCC CCAGACACCT GAGCTTCTCA 
GACGTGAGTC ATGATTCAGC CCGCGTGTCC TGGGAGGGCA CCGCAAGACC TGTGCGCCTG 
TTCAGGGTCA GCTACATCTC GGGCAAGGGA GGCCACTCGG GACAGACAGA GGTTCCTGGG 
AACGCCACCT CAGCCATCCT GGGGCCCCTC TCCTCCTCTA CCACCTACAT GGTCCGCGTC 
ACCTGTCTCT ACCCTGGGGG CAGCTCCTCT ACCCTGACCA GCCGCCTGAC CACACGGAAA 
GTCCCCAGCC CGAGCCAGCT GTCGGTGACA GAACTGCCTG GGGACGAAGT CCGGCTCGAG 
TGGGCGGCCG CGGCGGTGTC CGGCGTGCTT GTCTACCAGA TCACGTGGAT GCCCCTGGGA 
GAGGGGAAGG CCCGTGAGAT TTCTGTCCCG GGGAACCTGG GCACGGCCAT CCTGCCTGGC 
CTGGGGAGGC ACTCAGAGTA TGAGATCACC ATCCTGGCCT ACTACCGGGA CGGGGCCCGC 
AGTGACCCTG TGTCCCTCCG TTACACCCCC GTCAGCCGGA GCCCACCCTC CAACCTGGCC 
TTGGCCTCAG AGTCGCCTGA CAGCCTGCGG GTCAGCTGGA CGCCCCCGAG CAGTCACGTG 
CTCCACTACC GGCTCTCCTA CGCGCTGGCC TCAGGCTCTG GACCCGAGAA GTCGATCTCC 
ATTCCAGGAC CCGGGAGCCA CGTGACGATC CCTGACCTGC TGGCGGCTAC CAAGTACCGG 
GTCCTGGTCT CAGCCATCTA TGGAGCAGGG GAGAGCGTGG CGGTGTCTGC CACAGGCCGG 
ACGGCCTGCC CGGCCCTCCA CCTGGATGGC TCCCTCCCAG GCTTTGACCT GATGGCGGCC 
TTCGGGCTGG TGGAGAAAGA ATATGCCTCC ATCCGCGGCG TGGCCATGGA GCCCTCGGCC 
TTTGGCCCCA CCAGGACGTT CACACTCTTC AAGGACTCTC AGCTGACCCG GCGGGCCAGC 
GACATCCACC TGGCCACCCT GCCCCCGGAG CACACAGTCG TCTTCCTCCT GCGCCTGCTC 
CCTGAGACTC CCCGAGAGGC CTTTGCATTG TGGCAGGTGG CAAGCGAGGA CTTCCAGCCC 
ATCCTCGGGG TTCTGCTGGA CGCCGGCAGG AAGTCGCTGA CCTACTTCAA CCGTGACCCC 
AGGGCCACCT TGCAGGAGGT CACCTTTGAC CTGCCTGAAG TGAGGAGGAT ATTCTTTGGG 
AGTTTCCACA AGGTGCATGT GGCCGTGGGC CGAGCCAGGG TCAGGCTCTA CGTGGACTGC 
CGGAAGGTGG CCGAGAGGCC CATCGGGGAG GCCGGCGGCC TGCCTGCTGC CGGCTTCGTC 
ATGCTGGGCA GGTTGGCCAA AGCCCGGGGC CCCCGGAGCA GCTCCGCCTC GTTCCAGCTC 
CAGGTGCTAC AGCTCGTGTG CAATGACTCG TGGGCAGAGG AGGACAGGTG CTGTGAGCTC 
CCAGCATCGA AGGAGGGAGA GTCCTGTCCT GCCTTCCCAT CTGCCTGTGC ATGTTCCTCA 
CAGACCCCTG GGCCCCCAGG CCCCCAAGGA CCTCCGGGCC TCCCCGGGCG GAACGGAGCC 
CCAGGAGAGC AAGGCTTCCC AGGGCCCAGG GGAGAGCCCG GGCCACCTGG ACAGATGGGA 
CCCGAGGGTC CTGGGGGTCA GCAGGGGTCA CCGGGCACCC AGGGCCGCAC CATCCAGGGA 
CCCGTGGGTC CGCCAGGGGT CAAAGGAGAG AAGGGGGACC ACGGACACCC CGGCTTGCAG 
GGCCACCCCG GCCTCCAGGG CACCCCCGGG AAGATTGGCA TCCAGGGACC GAAGGGAATG 
AGAGGCCTGG AGGGGACTGC TGGACTGCCT GGCCCCCCAG GCCCTAGGGG CTTCCAAGGT 
ACTTCGGGGG CTAGGGGCAG CAACGGCGAG CGAGGACCCC CAGGGGCCGT GGGGCCCACA 
GGACTGCCGG GGCCCAAGGG GGAGCGAGGA GAGAAGGGAG AGCCACAGTC CCTGGCCACC 
ATCTACCAGC TCGTGGGCCA GGCTTGCGAG TCCGCCATCC AGACGCACGT GTTGAAGCTC 
CATGCCTGCA CCCACGAGAG CACCCGGCCC CCCATGCCCA TCTTGGAAGC CCCCAGGGCC 
CTTGGCACAA GCAACAAGGC CCGGCTCCCT GGAGAAGGGG GGCATGGTGG CCCCCACCCT 
GGGGACAGAG GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321587.1
CDS	79..3954
Translation	MSVRVRPHLGVGLCLYLSVTLGLSQGQASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPRAGDPEQEVMLTTKTPKATVGGLSPSKGYTLQIFELTGSGNILLARREFVIEDLKSNSVSRSSQRPLGATLEPTPSLVGSPDLEPPGALAPSQDPPTLGRPQFRCTPPMPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPGKVQVGLTQYSGAPQTEWDLNAFGTKEEVLNAVHNLHYRGGNTFTGLALTHVLEQNLKPRAGLRPEAAKLVILVTDGKSQDDAHTAGHVLKGLGVDIFAVGVKNADETELRLLASQPLDITVHNVQDFPQLSTLSGLLSRLICQKVQGRSPRGSPATPALDPPPAPTRLVLTQVTSSSVLLSWSPAPQPPLKYLIVWRPSKGGTPREVVVEGPTSSAQLHNLTSSTEYLVSVFPLYKAGVGEGLQGLATTAPLPPPQALTLAAVTPKTVRLTWQPSAEATQYLVRWSPASPKGEEERREVRVGRPEVLLDGLAPGRDYDVWVQSLRGAEISEAQGIRARTPALTTPRHLSFSDVSHDSARVSWEGTARPVRLFRVSYISGKGGHSGQTEVPGNATSAILGPLSSSTTYMVRVTCLYPGGSSSTLTSRLTTRKVPSPSQLSVTELPGDEVRLEWAAAAVSGVLVYQITWMPLGEGKAREISVPGNLGTAILPGLGRHSEYEITILAYYRDGARSDPVSLRYTPVSRSPPSNLALASESPDSLRVSWTPPSSHVLHYRLSYALASGSGPEKSISIPGPGSHVTIPDLLAATKYRVLVSAIYGAGESVAVSATGRTACPALHLDGSLPGFDLMAAFGLVEKEYASIRGVAMEPSAFGPTRTFTLFKDSQLTRRASDIHLATLPPEHTVVFLLRLLPETPREAFALWQVASEDFQPILGVLLDAGRKSLTYFNRDPRATLQEVTFDLPEVRRIFFGSFHKVHVAVGRARVRLYVDCRKVAERPIGEAGGLPAAGFVMLGRLAKARGPRSSSASFQLQVLQLVCNDSWAEEDRCCELPASKEGESCPAFPSACACSSQTPGPPGPQGPPGLPGRNGAPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTIQGPVGPPGVKGEKGDHGHPGLQGHPGLQGTPGKIGIQGPKGMRGLEGTAGLPGPPGPRGFQGTSGARGSNGERGPPGAVGPTGLPGPKGERGEKGEPQSLATIYQLVGQACESAIQTHVLKLHACTHESTRPPMPILEAPRALGTSNKARLPGEGGHGGPHPGDRGRSPCCLK

Target ORF information:

RefSeq Version	XM_015466101.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466101.1

ORF Insert Sequence:

ATGAGCGTCC GCGTGCGCCC TCACCTCGGA GTCGGCCTCT GTCTGTATCT GAGTGTCACC 
CTGGGGCTCA GCCAGGGACA AGCGAGTGGC CGCCTGAGGC TGGCGGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCAGAA GGGAGCAGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCAGGG CAGGGGACCC AGAGCAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACTGTGGGGG GCCTGAGCCC CTCCAAGGGG TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCAGGGAACA TCCTGCTGGC TCGCAGGGAG TTTGTGATCG AGGATCTGAA GAGTAACTCT 
GTGAGCAGGA GCAGCCAGAG GCCACTGGGG GCAACCCTGG AGCCCACCCC TTCCCTTGTG 
GGGAGCCCAG ACCTTGAGCC CCCAGGGGCC CTGGCCCCAA GCCAAGATCC ACCCACGCTT 
GGCAGACCCC AGTTCCGCTG CACACCCCCC ATGCCTGTGG ACATGATCTT CCTGGTGGAT 
GGGTCCTGGA GTATAGGCCA CAGTCACTTC CAGCAGGTCA AAGACTTCCT GGCCAGCGTC 
ATCGAGCCCT TTGAAATTGG GCCGGGCAAA GTCCAAGTAG GTCTGACTCA GTACAGTGGA 
GCCCCCCAGA CCGAGTGGGA CCTGAATGCC TTTGGCACCA AGGAGGAGGT GTTGAATGCT 
GTGCACAACC TCCACTACAG AGGAGGAAAC ACATTCACAG GCCTGGCCCT GACCCACGTG 
CTGGAGCAGA ACCTGAAGCC CAGAGCAGGT CTCCGTCCAG AGGCGGCCAA GCTGGTGATT 
CTGGTGACAG ATGGCAAGTC CCAGGATGAT GCCCACACTG CTGGCCACGT GCTCAAGGGT 
CTGGGTGTCG ACATCTTTGC TGTGGGCGTG AAGAATGCCG ACGAAACCGA GTTGAGGCTC 
CTGGCATCCC AGCCGCTGGA CATCACCGTC CACAATGTGC AGGACTTCCC CCAGCTCAGC 
ACACTCTCCG GCCTACTCAG CCGGCTCATC TGCCAGAAGG TCCAGGGCAG GAGCCCGCGT 
GGGAGCCCAG CTACACCGGC CCTGGATCCC CCTCCTGCTC CCACAAGGCT GGTCCTGACC 
CAAGTGACCT CCTCCAGCGT CCTCTTGTCC TGGAGCCCGG CCCCACAGCC GCCCCTCAAG 
TACCTGATTG TGTGGAGGCC TTCCAAGGGC GGCACCCCCA GGGAGGTGGT GGTGGAAGGG 
CCCACCTCGT CAGCACAGCT GCACAACCTG ACCTCCAGCA CCGAGTACCT GGTCTCTGTG 
TTCCCCCTCT ACAAGGCTGG CGTCGGCGAG GGCCTGCAGG GCCTGGCGAC CACAGCACCC 
CTGCCCCCAC CCCAGGCGCT GACCCTGGCT GCAGTGACAC CCAAAACTGT CCGCCTCACC 
TGGCAGCCCT CGGCAGAGGC CACCCAGTAC CTGGTGCGGT GGTCACCTGC CTCCCCCAAG 
GGCGAGGAGG AGAGGAGAGA GGTGCGGGTG GGGCGGCCGG AGGTGCTGCT GGATGGCCTG 
GCCCCGGGCA GGGACTATGA CGTGTGGGTG CAGAGCCTTC GAGGGGCAGA GATCAGTGAG 
GCCCAGGGCA TCCGTGCCAG GACCCCCGCC CTGACCACCC CCAGACACCT GAGCTTCTCA 
GACGTGAGTC ATGATTCAGC CCGCGTGTCC TGGGAGGGCA CCGCAAGACC TGTGCGCCTG 
TTCAGGGTCA GCTACATCTC GGGCAAGGGA GGCCACTCGG GACAGACAGA GGTTCCTGGG 
AACGCCACCT CAGCCATCCT GGGGCCCCTC TCCTCCTCTA CCACCTACAT GGTCCGCGTC 
ACCTGTCTCT ACCCTGGGGG CAGCTCCTCT ACCCTGACCA GCCGCCTGAC CACACGGAAA 
GTCCCCAGCC CGAGCCAGCT GTCGGTGACA GAACTGCCTG GGGACGAAGT CCGGCTCGAG 
TGGGCGGCCG CGGCGGTGTC CGGCGTGCTT GTCTACCAGA TCACGTGGAT GCCCCTGGGA 
GAGGGGAAGG CCCGTGAGAT TTCTGTCCCG GGGAACCTGG GCACGGCCAT CCTGCCTGGC 
CTGGGGAGGC ACTCAGAGTA TGAGATCACC ATCCTGGCCT ACTACCGGGA CGGGGCCCGC 
AGTGACCCTG TGTCCCTCCG TTACACCCCC GTCAGCCGGA GCCCACCCTC CAACCTGGCC 
TTGGCCTCAG AGTCGCCTGA CAGCCTGCGG GTCAGCTGGA CGCCCCCGAG CAGTCACGTG 
CTCCACTACC GGCTCTCCTA CGCGCTGGCC TCAGGCTCTG GACCCGAGAA GTCGATCTCC 
ATTCCAGGAC CCGGGAGCCA CGTGACGATC CCTGACCTGC TGGCGGCTAC CAAGTACCGG 
GTCCTGGTCT CAGCCATCTA TGGAGCAGGG GAGAGCGTGG CGGTGTCTGC CACAGGCCGG 
ACGGCCTGCC CGGCCCTCCA CCTGGATGGC TCCCTCCCAG GCTTTGACCT GATGGCGGCC 
TTCGGGCTGG TGGAGAAAGA ATATGCCTCC ATCCGCGGCG TGGCCATGGA GCCCTCGGCC 
TTTGGCCCCA CCAGGACGTT CACACTCTTC AAGGACTCTC AGCTGACCCG GCGGGCCAGC 
GACATCCACC TGGCCACCCT GCCCCCGGAG CACACAGTCG TCTTCCTCCT GCGCCTGCTC 
CCTGAGACTC CCCGAGAGGC CTTTGCATTG TGGCAGGTGG CAAGCGAGGA CTTCCAGCCC 
ATCCTCGGGG TTCTGCTGGA CGCCGGCAGG AAGTCGCTGA CCTACTTCAA CCGTGACCCC 
AGGGCCACCT TGCAGGAGGT CACCTTTGAC CTGCCTGAAG TGAGGAGGAT ATTCTTTGGG 
AGTTTCCACA AGGTGCATGT GGCCGTGGGC CGAGCCAGGG TCAGGCTCTA CGTGGACTGC 
CGGAAGGTGG CCGAGAGGCC CATCGGGGAG GCCGGCGGCC TGCCTGCTGC CGGCTTCGTC 
ATGCTGGGCA GGTTGGCCAA AGCCCGGGGC CCCCGGAGCA GCTCCGCCTC GTTCCAGCTC 
CAGGTGCTAC AGCTCGTGTG CAATGACTCG TGGGCAGAGG AGGACAGGTG CTGTGAGCTC 
CCAGCATCGA AGGAGGGAGA GTCCTGTCCT GCCTTCCCAT CTGCCTGTGC ATGTTCCTCA 
CAGACCCCTG GGCCCCCAGG CCCCCAAGGA CCTCCGGGCC TCCCCGGGCG GAACGGAGCC 
CCAGGAGAGC AAGGCTTCCC AGGGCCCAGG GGAGAGCCCG GGCCACCTGG ACAGATGGGA 
CCCGAGGGTC CTGGGGGTCA GCAGGGGTCA CCGGGCACCC AGGGCCGCAC CATCCAGGGA 
CCCGTGGGTC CGCCAGGGGT CAAAGGAGAG AAGGGGGACC ACGGACACCC CGGCTTGCAG 
GGCCACCCCG GCCTCCAGGG CACCCCCGGG AAGATTGGCA TCCAGGGACC GAAGGGAATG 
AGAGGCCTGG AGGGGACTGC TGGACTGCCT GGCCCCCCAG GCCCTAGGGG CTTCCAAGGT 
ACTTCGGGGG CTAGGGGCAG CAACGGCGAG CGAGGACCCC CAGGGGCCGT GGGGCCCACA 
GGACTGCCGG GGCCCAAGGG GGAGCGAGGA GAGAAGGGAG AGCCACAGTC CCTGGCCACC 
ATCTACCAGC TCGTGGGCCA GGCTTGCGAG TCCGCCATCC AGACGCACGT GTTGAAGCTC 
CATGCCTGCA CCCACGAGAG CACCCGGCCC CCCATGCCCA TCTTGGAAGC CCCCAGGGCC 
CTTGGCACAA GCAACAAGGC CCGGCTCCCT GGAGAAGGGG GGCATGGTGG CCCCCACCCT 
GGGGACAGAG GACGGTCCCC CTGCTGCCTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL20A1
Entrez Gene ID	519501
Full Name	collagen type XX alpha 1 chain
General protein information	Preferred Names collagen type XX alpha 1 chain Names collagen alpha-1(XX) chain collagen, type XX, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Col20a1	XP_006235874.1
Homo sapiens (human)	COL20A1	NP_065933.2
Pan troglodytes (chimpanzee)	COL20A1	XP_001148451.1
Bos taurus (cattle)	COL20A1	XP_002692297.2
Mus musculus (house mouse)	Col20a1	NP_082794.1
Gallus gallus (chicken)	COL20A1	NP_001004392.1
Canis lupus familiaris (dog)	COL20A1	XP_543096.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL20A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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