SPIRE2 cDNA ORF clone, Bos taurus(cattle)

The following SPIRE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPIRE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa35970 NM_001205387.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225006 XM_024978241.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225007 XM_024978242.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225008 XM_024978244.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225009 XM_024978245.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225010 XM_024978246.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225011 XM_024978247.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225012 XM_024978248.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225013 XM_024978249.1
Latest version!
Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa35970
    Clone ID Related Accession (Same CDS sequence) NM_001205387.1
    Accession Version NM_001205387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product protein spire homolog 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000018.1, CK831845.1, EV683836.1, DV914571.1, DN522737.1 and EV747125.1. On or before Aug 31, 2013 this sequence version replaced XM_003587206.2, XM_001787738.1, XM_002694865.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571263.36181.1, SRR5571258.4640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGGT
    TCCCTCCCCG TCAGTGTCCA GTCCCAGCCT GACCCCAGCT CCCTACCACG CAGTGACATG
    AGCTCAGTCG AGGACAGGCA GGGGGCTTCC GCGGCCCCGG ACGCCAGTCA CCTGTGGCTG
    GAGTTCAGCC ACCCCGTGGA AAGCCTGGCT CTGACGGTAG AGGAGGTGAT GGACGTGCGC
    CGTGTGCTGG TGAAGGCAGA GATGGAGAAG TTCCTGCAGA ACAAGGAGCT CTTCAGCAGT
    CTGAAGAAGG GGAAGATTTG CTGCTGCTGC CGAGCCAAGT TCCCGCTGTT GTCATGGCCA
    CCCTCCTGTC TCTTTTGCAA GAGAGCCGTC TGCAACTCCT GTAGCATAAA GATGAAGATG
    CCTTCTAAGA AGTTTGCTCA CATCCCTGTC TACACACTGG GCTTTGAGAG TCCTCAGAGG
    ATGTCAACTG CCAAAACCAC ACCAACACAG AGAAGAGACG CCTTCCAATC CCTGCAAGGG
    CTGCAGTGGC GGAACGTGGA GGAGGAGTTC CCGCACATGT ACACCCACGG CTGTGTCATG
    AAGGACGTGT GCAGCGACTG CACCGGGTTC GTGGCGGATG TGGTGCACTC CAGCCGCAGG
    AGCGTGGAGG CCCTCAACAC CACGCCACGT CGTGCCCGCC AGACCCAGTC CCTGTATGTC
    CCGAACACCT GGACACTTGA CCTCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192316.1
    CDS54..2243
    Translation

    Target ORF information:

    RefSeq Version NM_001205387.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGGT
    TCCCTCCCCG TCAGTGTCCA GTCCCAGCCT GACCCCAGCT CCCTACCACG CAGTGACATG
    AGCTCAGTCG AGGACAGGCA GGGGGCTTCC GCGGCCCCGG ACGCCAGTCA CCTGTGGCTG
    GAGTTCAGCC ACCCCGTGGA AAGCCTGGCT CTGACGGTAG AGGAGGTGAT GGACGTGCGC
    CGTGTGCTGG TGAAGGCAGA GATGGAGAAG TTCCTGCAGA ACAAGGAGCT CTTCAGCAGT
    CTGAAGAAGG GGAAGATTTG CTGCTGCTGC CGAGCCAAGT TCCCGCTGTT GTCATGGCCA
    CCCTCCTGTC TCTTTTGCAA GAGAGCCGTC TGCAACTCCT GTAGCATAAA GATGAAGATG
    CCTTCTAAGA AGTTTGCTCA CATCCCTGTC TACACACTGG GCTTTGAGAG TCCTCAGAGG
    ATGTCAACTG CCAAAACCAC ACCAACACAG AGAAGAGACG CCTTCCAATC CCTGCAAGGG
    CTGCAGTGGC GGAACGTGGA GGAGGAGTTC CCGCACATGT ACACCCACGG CTGTGTCATG
    AAGGACGTGT GCAGCGACTG CACCGGGTTC GTGGCGGATG TGGTGCACTC CAGCCGCAGG
    AGCGTGGAGG CCCTCAACAC CACGCCACGT CGTGCCCGCC AGACCCAGTC CCTGTATGTC
    CCGAACACCT GGACACTTGA CCTCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225006
    Clone ID Related Accession (Same CDS sequence) XM_024978241.1
    Accession Version XM_024978241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGAGCCG TCTGCAACTC CTGTAGCATA AAGATGAAGA TGCCTTCTAA GAAGTTTGCT
    CACATCCCTG TCTACACACT GGGCTTTGAG AGTCCTCAGA GGATGTCAAC TGCCAAAACC
    ACACCAACAC AGAGAAGAGA CGCCTTCCAG TGCGTGCTGC CCTTGATCCC TGCAAGGGCT
    GCAGTGGCGG AACGTGGAGG AGGAGTTCCC GCACATGTAC ACCCACGGCT GTGTCATGAA
    GGACGTGTGC AGCGACTGCA CCGGGTTCGT GGCGGATGTG GTGCACTCCA GCCGCAGGAG
    CGTGGAGGCC CTCAACACCA CGCCACGTCG TGCCCGCCAG ACCCAGTCCC TGTATGTCCC
    GAACACCTGG ACACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834009.1
    CDS52..2349
    Translation

    Target ORF information:

    RefSeq Version XM_024978241.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGAGCCG TCTGCAACTC CTGTAGCATA AAGATGAAGA TGCCTTCTAA GAAGTTTGCT
    CACATCCCTG TCTACACACT GGGCTTTGAG AGTCCTCAGA GGATGTCAAC TGCCAAAACC
    ACACCAACAC AGAGAAGAGA CGCCTTCCAG TGCGTGCTGC CCTTGATCCC TGCAAGGGCT
    GCAGTGGCGG AACGTGGAGG AGGAGTTCCC GCACATGTAC ACCCACGGCT GTGTCATGAA
    GGACGTGTGC AGCGACTGCA CCGGGTTCGT GGCGGATGTG GTGCACTCCA GCCGCAGGAG
    CGTGGAGGCC CTCAACACCA CGCCACGTCG TGCCCGCCAG ACCCAGTCCC TGTATGTCCC
    GAACACCTGG ACACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225007
    Clone ID Related Accession (Same CDS sequence) XM_024978242.1
    Accession Version XM_024978242.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGAGCCG TCTGCAACTC CTGTAGCATA AAGATGAAGA TGCCTTCTAA GAAGTTTGCT
    CACATCCCTG TCTACACACT GGGCTTTGAG AGTCCTCAGA GGATGTCAAC TGCCAAAACC
    ACACCAACAC AGAGAAGAGA CGCCTTCCAA TCCCTGCAAG GGCTGCAGTG GCGGAACGTG
    GAGGAGGAGT TCCCGCACAT GTACACCCAC GGCTGTGTCA TGAAGGACGT GTGCAGCGAC
    TGCACCGGGT TCGTGGCGGA TGTGGTGCAC TCCAGCCGCA GGAGCGTGGA GGCCCTCAAC
    ACCACGCCAC GTCGTGCCCG CCAGACCCAG TCCCTGTATG TCCCGAACAC CTGGACACTT
    GACCTCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834010.1
    CDS49..2340
    Translation

    Target ORF information:

    RefSeq Version XM_024978242.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGAGCCG TCTGCAACTC CTGTAGCATA AAGATGAAGA TGCCTTCTAA GAAGTTTGCT
    CACATCCCTG TCTACACACT GGGCTTTGAG AGTCCTCAGA GGATGTCAAC TGCCAAAACC
    ACACCAACAC AGAGAAGAGA CGCCTTCCAA TCCCTGCAAG GGCTGCAGTG GCGGAACGTG
    GAGGAGGAGT TCCCGCACAT GTACACCCAC GGCTGTGTCA TGAAGGACGT GTGCAGCGAC
    TGCACCGGGT TCGTGGCGGA TGTGGTGCAC TCCAGCCGCA GGAGCGTGGA GGCCCTCAAC
    ACCACGCCAC GTCGTGCCCG CCAGACCCAG TCCCTGTATG TCCCGAACAC CTGGACACTT
    GACCTCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225008
    Clone ID Related Accession (Same CDS sequence) XM_024978244.1
    Accession Version XM_024978244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGAGCCCACA ACAATGGTGT CACCGGCCAG CTCGGAAGCC
    CAGGTGGTGC AGTCGCTGGG CTTCGCCATC TACCGCGCGC TGGACTGGGG GCTGGCCGAG
    CACGAGGAGC GCGAGCTCAG CCCGCAGCTG GAGCGGCTCA TCGACCTCAT GGCCAACAGC
    GACTGCGATG ACGACGGCGG TGGCGGGACG GCCGACGAAG GATACGGGGG TCCCGAGGAG
    GAAGAGGAGG CAGAGGGCGG CCCCCGCACG GTGCGCACCT TCGCGCAGGC TATGCGCCTG
    TGTGCCGCGC GCCTGACCGA GCCCCGGGGC GCCCAGACCC ACTACCAGGC CGTGTGCCGC
    GCGCTCTTCG TGGAGACGCT GGAACTGCGC GCCTTCCTCG CCAGGGTCCG GGAAGCCAAG
    GAGATGCTGC AGAGGTTCCG GGAGGATGAA CCGCAGGCAG AGAAGCCCCT TGTGGAGCTG
    GATAGCCTGA GGCGCACAGA CTGGGCCCGC CTCTGGGTCC AGCTCATGCG GGAGCTCCGC
    CATGGGGTGA AGCTGAAGAA GGTGCAGGAG CAGGAGTTCA ACCCCCTGCC CACAGAGTTC
    CAGCTCACCC CCTTCGAGAT GCTCATGCAG GACATCCGTG CCAGGAACTA CAAACTGCGC
    AAGGTCATGG TTGACGGGGA CATCCCTCCC AGAGTGAAGA AAGATGCCCA TGAACTCATC
    CTGGACTTCA TCCGCTCCCG GCCTCCACTG AAGCAGGTCT CAGAGAGAAG GCTTCGCCCC
    TTACCCCAGA AGCAGAGAAC TCTGCACGAG AAGATCCTGG AGGAGATCAA GCAGGAACGC
    AGGCTACGCC CCGTGGAGAG CAGGCGCTGG GACGGCCGGG GCGAGCGGGG GTTCGGCTCT
    CTGCCCTGCA TCCTCAACGC CTGCTCTGGG GACGTCAAAT CCACTTCCTG TATCAACCTG
    TCCATCACGG ATGTTGGGAG CAGCGCCCAG CGCCCGAGGC CCCGGGTCCT GCTCAAGGCA
    CCTACGTTGG CTGAGATGGA AGAGATGAAT ACTTCTGAGG AGGAAGAGTC ACCGTGCGGA
    GAGGGGACGC TGAAGCGAGA TCGTTCTTTT TCTGAGCATG ACCTGGTGCA GCTCCGGAGT
    GAGGTCACCT CTGGTCTGCA GCCGGCCCCT CAGCCCCCCG GAGGCCTGGA GTTGTCCCGG
    CCGCGAGCAG GCAGCATGCA GTCCTGGAGG CCGAGCCCCC TGGAGCCGGT GGAAAGGGGC
    CCAGAGAGCA TGCACACCAC TCTGGACTGG ACTAGTCCCC TGCGGTTTGT CACAGCCCCG
    TCCCTTCCCG TCGGTCAGCC AGCCACTCTA GGTTCCCTCC CCGTCAGTGT CCAGTCCCAG
    CCTGACCCCA GCTCCCTACC ACGCAGTGAC ATGAGCTCAG TCGAGGACAG GCAGGGGGCT
    TCCGCGGCCC CGGACGCCAG TCACCTGTGG CTGGAGTTCA GCCACCCCGT GGAAAGCCTG
    GCTCTGACGG TAGAGGAGGT GATGGACGTG CGCCGTGTGC TGGTGAAGGC AGAGATGGAG
    AAGTTCCTGC AGAACAAGGA GCTCTTCAGC AGTCTGAAGA AGGGGAAGAT TTGCTGCTGC
    TGCCGAGCCA AGTTCCCGCT GTTGTCATGG CCACCCTCCT GTCTCTTTTG CAAGAGAGCC
    GTCTGCAACT CCTGTAGCAT AAAGATGAAG ATGCCTTCTA AGAAGTTTGC TCACATCCCT
    GTCTACACAC TGGGCTTTGA GAGTCCTCAG AGGATGTCAA CTGCCAAAAC CACACCAACA
    CAGAGAAGAG ACGCCTTCCA GTGCGTGCTG CCCTTGATCC CTGCAAGGGC TGCAGTGGCG
    GAACGTGGAG GAGGAGTTCC CGCACATGTA CACCCACGGC TGTGTCATGA AGGACGTGTG
    CAGCGACTGC ACCGGGTTCG TGGCGGATGT GGTGCACTCC AGCCGCAGGA GCGTGGAGGC
    CCTCAACACC ACGCCACGTC GTGCCCGCCA GACCCAGTCC CTGTATGTCC CGAACACCTG
    GACACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834012.1
    CDS53..2341
    Translation

    Target ORF information:

    RefSeq Version XM_024978244.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGAGCCCACA ACAATGGTGT CACCGGCCAG CTCGGAAGCC
    CAGGTGGTGC AGTCGCTGGG CTTCGCCATC TACCGCGCGC TGGACTGGGG GCTGGCCGAG
    CACGAGGAGC GCGAGCTCAG CCCGCAGCTG GAGCGGCTCA TCGACCTCAT GGCCAACAGC
    GACTGCGATG ACGACGGCGG TGGCGGGACG GCCGACGAAG GATACGGGGG TCCCGAGGAG
    GAAGAGGAGG CAGAGGGCGG CCCCCGCACG GTGCGCACCT TCGCGCAGGC TATGCGCCTG
    TGTGCCGCGC GCCTGACCGA GCCCCGGGGC GCCCAGACCC ACTACCAGGC CGTGTGCCGC
    GCGCTCTTCG TGGAGACGCT GGAACTGCGC GCCTTCCTCG CCAGGGTCCG GGAAGCCAAG
    GAGATGCTGC AGAGGTTCCG GGAGGATGAA CCGCAGGCAG AGAAGCCCCT TGTGGAGCTG
    GATAGCCTGA GGCGCACAGA CTGGGCCCGC CTCTGGGTCC AGCTCATGCG GGAGCTCCGC
    CATGGGGTGA AGCTGAAGAA GGTGCAGGAG CAGGAGTTCA ACCCCCTGCC CACAGAGTTC
    CAGCTCACCC CCTTCGAGAT GCTCATGCAG GACATCCGTG CCAGGAACTA CAAACTGCGC
    AAGGTCATGG TTGACGGGGA CATCCCTCCC AGAGTGAAGA AAGATGCCCA TGAACTCATC
    CTGGACTTCA TCCGCTCCCG GCCTCCACTG AAGCAGGTCT CAGAGAGAAG GCTTCGCCCC
    TTACCCCAGA AGCAGAGAAC TCTGCACGAG AAGATCCTGG AGGAGATCAA GCAGGAACGC
    AGGCTACGCC CCGTGGAGAG CAGGCGCTGG GACGGCCGGG GCGAGCGGGG GTTCGGCTCT
    CTGCCCTGCA TCCTCAACGC CTGCTCTGGG GACGTCAAAT CCACTTCCTG TATCAACCTG
    TCCATCACGG ATGTTGGGAG CAGCGCCCAG CGCCCGAGGC CCCGGGTCCT GCTCAAGGCA
    CCTACGTTGG CTGAGATGGA AGAGATGAAT ACTTCTGAGG AGGAAGAGTC ACCGTGCGGA
    GAGGGGACGC TGAAGCGAGA TCGTTCTTTT TCTGAGCATG ACCTGGTGCA GCTCCGGAGT
    GAGGTCACCT CTGGTCTGCA GCCGGCCCCT CAGCCCCCCG GAGGCCTGGA GTTGTCCCGG
    CCGCGAGCAG GCAGCATGCA GTCCTGGAGG CCGAGCCCCC TGGAGCCGGT GGAAAGGGGC
    CCAGAGAGCA TGCACACCAC TCTGGACTGG ACTAGTCCCC TGCGGTTTGT CACAGCCCCG
    TCCCTTCCCG TCGGTCAGCC AGCCACTCTA GGTTCCCTCC CCGTCAGTGT CCAGTCCCAG
    CCTGACCCCA GCTCCCTACC ACGCAGTGAC ATGAGCTCAG TCGAGGACAG GCAGGGGGCT
    TCCGCGGCCC CGGACGCCAG TCACCTGTGG CTGGAGTTCA GCCACCCCGT GGAAAGCCTG
    GCTCTGACGG TAGAGGAGGT GATGGACGTG CGCCGTGTGC TGGTGAAGGC AGAGATGGAG
    AAGTTCCTGC AGAACAAGGA GCTCTTCAGC AGTCTGAAGA AGGGGAAGAT TTGCTGCTGC
    TGCCGAGCCA AGTTCCCGCT GTTGTCATGG CCACCCTCCT GTCTCTTTTG CAAGAGAGCC
    GTCTGCAACT CCTGTAGCAT AAAGATGAAG ATGCCTTCTA AGAAGTTTGC TCACATCCCT
    GTCTACACAC TGGGCTTTGA GAGTCCTCAG AGGATGTCAA CTGCCAAAAC CACACCAACA
    CAGAGAAGAG ACGCCTTCCA GTGCGTGCTG CCCTTGATCC CTGCAAGGGC TGCAGTGGCG
    GAACGTGGAG GAGGAGTTCC CGCACATGTA CACCCACGGC TGTGTCATGA AGGACGTGTG
    CAGCGACTGC ACCGGGTTCG TGGCGGATGT GGTGCACTCC AGCCGCAGGA GCGTGGAGGC
    CCTCAACACC ACGCCACGTC GTGCCCGCCA GACCCAGTCC CTGTATGTCC CGAACACCTG
    GACACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225009
    Clone ID Related Accession (Same CDS sequence) XM_024978245.1
    Accession Version XM_024978245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGGT
    TCCCTCCCCG TCAGTGTCCA GTCCCAGCCT GACCCCAGCT CCCTACCACG CAGTGACATG
    AGCTCAGTCG AGGACAGGCA GGGGGCTTCC GCGGCCCCGG ACGCCAGTCA CCTGTGGCTG
    GAGTTCAGCC ACCCCGTGGA AAGCCTGGCT CTGACGGTAG AGGAGGTGAT GGACGTGCGC
    CGTGTGCTGG TGAAGGCAGA GATGGAGAAG TTCCTGCAGA ACAAGGAGCT CTTCAGCAGT
    CTGAAGAAGG GGAAGATTTG CTGCTGCTGC CGAGCCAAGT TCCCGCTGTT GTCATGGCCA
    CCCTCCTGTC TCTTTTGCAA GAGAGCCGTC TGCAACTCCT GTAGCATAAA GATGAAGATG
    CCTTCTAAGA AGTTTGCTCA CATCCCTGTC TACACACTGG GCTTTGAGAG TCCTCAGAGG
    ATGTCAACTG CCAAAACCAC ACCAACACAG AGAAGAGACG CCTTCCAGTG CGTGCTGCCC
    TTGATCCCTG CAAGGGCTGC AGTGGCGGAA CGTGGAGGAG GAGTTCCCGC ACATGTACAC
    CCACGGCTGT GTCATGAAGG ACGTGTGCAG CGACTGCACC GGGTTCGTGG CGGATGTGGT
    GCACTCCAGC CGCAGGAGCG TGGAGGCCCT CAACACCACG CCACGTCGTG CCCGCCAGAC
    CCAGTCCCTG TATGTCCCGA ACACCTGGAC ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834013.1
    CDS73..2268
    Translation

    Target ORF information:

    RefSeq Version XM_024978245.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGGT
    TCCCTCCCCG TCAGTGTCCA GTCCCAGCCT GACCCCAGCT CCCTACCACG CAGTGACATG
    AGCTCAGTCG AGGACAGGCA GGGGGCTTCC GCGGCCCCGG ACGCCAGTCA CCTGTGGCTG
    GAGTTCAGCC ACCCCGTGGA AAGCCTGGCT CTGACGGTAG AGGAGGTGAT GGACGTGCGC
    CGTGTGCTGG TGAAGGCAGA GATGGAGAAG TTCCTGCAGA ACAAGGAGCT CTTCAGCAGT
    CTGAAGAAGG GGAAGATTTG CTGCTGCTGC CGAGCCAAGT TCCCGCTGTT GTCATGGCCA
    CCCTCCTGTC TCTTTTGCAA GAGAGCCGTC TGCAACTCCT GTAGCATAAA GATGAAGATG
    CCTTCTAAGA AGTTTGCTCA CATCCCTGTC TACACACTGG GCTTTGAGAG TCCTCAGAGG
    ATGTCAACTG CCAAAACCAC ACCAACACAG AGAAGAGACG CCTTCCAGTG CGTGCTGCCC
    TTGATCCCTG CAAGGGCTGC AGTGGCGGAA CGTGGAGGAG GAGTTCCCGC ACATGTACAC
    CCACGGCTGT GTCATGAAGG ACGTGTGCAG CGACTGCACC GGGTTCGTGG CGGATGTGGT
    GCACTCCAGC CGCAGGAGCG TGGAGGCCCT CAACACCACG CCACGTCGTG CCCGCCAGAC
    CCAGTCCCTG TATGTCCCGA ACACCTGGAC ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225010
    Clone ID Related Accession (Same CDS sequence) XM_024978246.1
    Accession Version XM_024978246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGATTAT CAGGCATGTC AAGAAGCAAG AAAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834014.1
    CDS56..2014
    Translation

    Target ORF information:

    RefSeq Version XM_024978246.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGATTAT CAGGCATGTC AAGAAGCAAG AAAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225011
    Clone ID Related Accession (Same CDS sequence) XM_024978247.1
    Accession Version XM_024978247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834015.1
    CDS55..1983
    Translation

    Target ORF information:

    RefSeq Version XM_024978247.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225012
    Clone ID Related Accession (Same CDS sequence) XM_024978248.1
    Accession Version XM_024978248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834016.1
    CDS55..1983
    Translation

    Target ORF information:

    RefSeq Version XM_024978248.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGGTGCGGCG GAGCCCACAA CAATGGTGTC ACCGGCCAGC
    TCGGAAGCCC AGGTGGTGCA GTCGCTGGGC TTCGCCATCT ACCGCGCGCT GGACTGGGGG
    CTGGCCGAGC ACGAGGAGCG CGAGCTCAGC CCGCAGCTGG AGCGGCTCAT CGACCTCATG
    GCCAACAGCG ACTGCGATGA CGACGGCGGT GGCGGGACGG CCGACGAAGG ATACGGGGGT
    CCCGAGGAGG AAGAGGAGGC AGAGGGCGGC CCCCGCACGG TGCGCACCTT CGCGCAGGCT
    ATGCGCCTGT GTGCCGCGCG CCTGACCGAG CCCCGGGGCG CCCAGACCCA CTACCAGGCC
    GTGTGCCGCG CGCTCTTCGT GGAGACGCTG GAACTGCGCG CCTTCCTCGC CAGGGTCCGG
    GAAGCCAAGG AGATGCTGCA GAGGTTCCGG GAGGATGAAC CGCAGGCAGA GAAGCCCCTT
    GTGGAGCTGG ATAGCCTGAG GCGCACAGAC TGGGCCCGCC TCTGGGTCCA GCTCATGCGG
    GAGCTCCGCC ATGGGGTGAA GCTGAAGAAG GTGCAGGAGC AGGAGTTCAA CCCCCTGCCC
    ACAGAGTTCC AGCTCACCCC CTTCGAGATG CTCATGCAGG ACATCCGTGC CAGGAACTAC
    AAACTGCGCA AGGTCATGGT TGACGGGGAC ATCCCTCCCA GAGTGAAGAA AGATGCCCAT
    GAACTCATCC TGGACTTCAT CCGCTCCCGG CCTCCACTGA AGCAGGTCTC AGAGAGAAGG
    CTTCGCCCCT TACCCCAGAA GCAGAGAACT CTGCACGAGA AGATCCTGGA GGAGATCAAG
    CAGGAACGCA GGCTACGCCC CGTGGAGAGC AGGCGCTGGG ACGGCCGGGG CGAGCGGGGG
    TTCGGCTCTC TGCCCTGCAT CCTCAACGCC TGCTCTGGGG ACGTCAAATC CACTTCCTGT
    ATCAACCTGT CCATCACGGA TGTTGGGAGC AGCGCCCAGC GCCCGAGGCC CCGGGTCCTG
    CTCAAGGCAC CTACGTTGGC TGAGATGGAA GAGATGAATA CTTCTGAGGA GGAAGAGTCA
    CCGTGCGGAG AGGGGACGCT GAAGCGAGAT CGTTCTTTTT CTGAGCATGA CCTGGTGCAG
    CTCCGGAGTG AGGTCACCTC TGGTCTGCAG CCGGCCCCTC AGCCCCCCGG AGGCCTGGAG
    TTGTCCCGGC CGCGAGCAGG CAGCATGCAG TCCTGGAGGC CGAGCCCCCT GGAGCCGGTG
    GAAAGGGGCC CAGAGAGCAT GCACACCACT CTGGACTGGA CTAGTCCCCT GCGGTTTGTC
    ACAGCCCCGT CCCTTCCCGT CGGTCAGCCA GCCACTCTAG GTTCCCTCCC CGTCAGTGTC
    CAGTCCCAGC CTGACCCCAG CTCCCTACCA CGCAGTGACA TGAGCTCAGT CGAGGACAGG
    CAGGGGGCTT CCGCGGCCCC GGACGCCAGT CACCTGTGGC TGGAGTTCAG CCACCCCGTG
    GAAAGCCTGG CTCTGACGGT AGAGGAGGTG ATGGACGTGC GCCGTGTGCT GGTGAAGGCA
    GAGATGGAGA AGTTCCTGCA GAACAAGGAG CTCTTCAGCA GTCTGAAGAA GGGGAAGATT
    TGCTGCTGCT GCCGAGCCAA GTTCCCGCTG TTGTCATGGC CACCCTCCTG TCTCTTTTGC
    AAGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225013
    Clone ID Related Accession (Same CDS sequence) XM_024978249.1
    Accession Version XM_024978249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein spire homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGAGCCCACA ACAATGGTGT CACCGGCCAG CTCGGAAGCC
    CAGGTGGTGC AGTCGCTGGG CTTCGCCATC TACCGCGCGC TGGACTGGGG GCTGGCCGAG
    CACGAGGAGC GCGAGCTCAG CCCGCAGCTG GAGCGGCTCA TCGACCTCAT GGCCAACAGC
    GACTGCGATG ACGACGGCGG TGGCGGGACG GCCGACGAAG GATACGGGGG TCCCGAGGAG
    GAAGAGGAGG CAGAGGGCGG CCCCCGCACG GTGCGCACCT TCGCGCAGGC TATGCGCCTG
    TGTGCCGCGC GCCTGACCGA GCCCCGGGGC GCCCAGACCC ACTACCAGGC CGTGTGCCGC
    GCGCTCTTCG TGGAGACGCT GGAACTGCGC GCCTTCCTCG CCAGGGTCCG GGAAGCCAAG
    GAGATGCTGC AGAGGTTCCG GGAGGATGAA CCGCAGGCAG AGAAGCCCCT TGTGGAGCTG
    GATAGCCTGA GGCGCACAGA CTGGGCCCGC CTCTGGGTCC AGCTCATGCG GGAGCTCCGC
    CATGGGGTGA AGCTGAAGAA GGTGCAGGAG CAGGAGTTCA ACCCCCTGCC CACAGAGTTC
    CAGCTCACCC CCTTCGAGAT GCTCATGCAG GACATCCGTG CCAGGAACTA CAAACTGCGC
    AAGGTCATGG TTGACGGGGA CATCCCTCCC AGAGTGAAGA AAGATGCCCA TGAACTCATC
    CTGGACTTCA TCCGCTCCCG GCCTCCACTG AAGCAGGTCT CAGAGAGAAG GCTTCGCCCC
    TTACCCCAGA AGCAGAGAAC TCTGCACGAG AAGATCCTGG AGGAGATCAA GCAGGAACGC
    AGGCTACGCC CCGTGGAGAG CAGGCGCTGG GACGGCCGGG GCGAGCGGGG GTTCGGCTCT
    CTGCCCTGCA TCCTCAACGC CTGCTCTGGG GACGTCAAAT CCACTTCCTG TATCAACCTG
    TCCATCACGG ATGTTGGGAG CAGCGCCCAG CGCCCGAGGC CCCGGGTCCT GCTCAAGGCA
    CCTACGTTGG CTGAGATGGA AGAGATGAAT ACTTCTGAGG AGGAAGAGTC ACCGTGCGGA
    GAGGGGACGC TGAAGCGAGA TCGTTCTTTT TCTGAGCATG ACCTGGTGCA GCTCCGGAGT
    GAGGTCACCT CTGGTCTGCA GCCGGCCCCT CAGCCCCCCG GAGGCCTGGA GTTGTCCCGG
    CCGCGAGCAG GCAGCATGCA GTCCTGGAGG CCGAGCCCCC TGGAGCCGGG TTCCCTCCCC
    GTCAGTGTCC AGTCCCAGCC TGACCCCAGC TCCCTACCAC GCAGTGACAT GAGCTCAGTC
    GAGGACAGGC AGGGGGCTTC CGCGGCCCCG GACGCCAGTC ACCTGTGGCT GGAGTTCAGC
    CACCCCGTGG AAAGCCTGGC TCTGACGGTA GAGGAGGTGA TGGACGTGCG CCGTGTGCTG
    GTGAAGGCAG AGATGGAGAA GTTCCTGCAG AACAAGGAGC TCTTCAGCAG TCTGAAGAAG
    GGGAAGATTT GCTGCTGCTG CCGAGCCAAG TTCCCGCTGT TGTCATGGCC ACCCTCCTGT
    CTCTTTTGCA AGAGAGCCGT CTGCAACTCC TGTAGCATAA AGATGAAGAT GCCTTCTAAG
    AAGTTTGCTC ACATCCCTGT CTACACACTG GGCTTTGAGA GTCCTCAGAG GATGTCAACT
    GCCAAAACCA CACCAACACA GAGAAGAGAC GCCTTCCAAT CCCTGCAAGG GCTGCAGTGG
    CGGAACGTGG AGGAGGAGTT CCCGCACATG TACACCCACG GCTGTGTCAT GAAGGACGTG
    TGCAGCGACT GCACCGGGTT CGTGGCGGAT GTGGTGCACT CCAGCCGCAG GAGCGTGGAG
    GCCCTCAACA CCACGCCACG TCGTGCCCGC CAGACCCAGT CCCTGTATGT CCCGAACACC
    TGGACACTTG ACCTCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834017.1
    CDS46..2226
    Translation

    Target ORF information:

    RefSeq Version XM_024978249.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spire type actin nucleation factor 2 (SPIRE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCCGGG CCGGCAGCTC CAGCGGCGAC GCGGCCGCGG GCGAGCGGGC CGGGGGCGCG 
    GGGCGCTCCG AGCCGTGGGA GCTGTCCCTG GAGGAGGTGC TGAAGGCGTA CGAGCAGCCC
    ATCAACGAGG AGCAGGCGTG GGCCGTGTGC TTCCAGTGCT GCCGCGGGCT GCGGGGCGCG
    CCGGGCGGCC GGCGGCGCAT CCGGGACACC GCGGACATCC TCCTGCGCAG AGACGGCTCG
    GTCAGCGCGC GGCGGGAGTC CGAGCCCACA ACAATGGTGT CACCGGCCAG CTCGGAAGCC
    CAGGTGGTGC AGTCGCTGGG CTTCGCCATC TACCGCGCGC TGGACTGGGG GCTGGCCGAG
    CACGAGGAGC GCGAGCTCAG CCCGCAGCTG GAGCGGCTCA TCGACCTCAT GGCCAACAGC
    GACTGCGATG ACGACGGCGG TGGCGGGACG GCCGACGAAG GATACGGGGG TCCCGAGGAG
    GAAGAGGAGG CAGAGGGCGG CCCCCGCACG GTGCGCACCT TCGCGCAGGC TATGCGCCTG
    TGTGCCGCGC GCCTGACCGA GCCCCGGGGC GCCCAGACCC ACTACCAGGC CGTGTGCCGC
    GCGCTCTTCG TGGAGACGCT GGAACTGCGC GCCTTCCTCG CCAGGGTCCG GGAAGCCAAG
    GAGATGCTGC AGAGGTTCCG GGAGGATGAA CCGCAGGCAG AGAAGCCCCT TGTGGAGCTG
    GATAGCCTGA GGCGCACAGA CTGGGCCCGC CTCTGGGTCC AGCTCATGCG GGAGCTCCGC
    CATGGGGTGA AGCTGAAGAA GGTGCAGGAG CAGGAGTTCA ACCCCCTGCC CACAGAGTTC
    CAGCTCACCC CCTTCGAGAT GCTCATGCAG GACATCCGTG CCAGGAACTA CAAACTGCGC
    AAGGTCATGG TTGACGGGGA CATCCCTCCC AGAGTGAAGA AAGATGCCCA TGAACTCATC
    CTGGACTTCA TCCGCTCCCG GCCTCCACTG AAGCAGGTCT CAGAGAGAAG GCTTCGCCCC
    TTACCCCAGA AGCAGAGAAC TCTGCACGAG AAGATCCTGG AGGAGATCAA GCAGGAACGC
    AGGCTACGCC CCGTGGAGAG CAGGCGCTGG GACGGCCGGG GCGAGCGGGG GTTCGGCTCT
    CTGCCCTGCA TCCTCAACGC CTGCTCTGGG GACGTCAAAT CCACTTCCTG TATCAACCTG
    TCCATCACGG ATGTTGGGAG CAGCGCCCAG CGCCCGAGGC CCCGGGTCCT GCTCAAGGCA
    CCTACGTTGG CTGAGATGGA AGAGATGAAT ACTTCTGAGG AGGAAGAGTC ACCGTGCGGA
    GAGGGGACGC TGAAGCGAGA TCGTTCTTTT TCTGAGCATG ACCTGGTGCA GCTCCGGAGT
    GAGGTCACCT CTGGTCTGCA GCCGGCCCCT CAGCCCCCCG GAGGCCTGGA GTTGTCCCGG
    CCGCGAGCAG GCAGCATGCA GTCCTGGAGG CCGAGCCCCC TGGAGCCGGG TTCCCTCCCC
    GTCAGTGTCC AGTCCCAGCC TGACCCCAGC TCCCTACCAC GCAGTGACAT GAGCTCAGTC
    GAGGACAGGC AGGGGGCTTC CGCGGCCCCG GACGCCAGTC ACCTGTGGCT GGAGTTCAGC
    CACCCCGTGG AAAGCCTGGC TCTGACGGTA GAGGAGGTGA TGGACGTGCG CCGTGTGCTG
    GTGAAGGCAG AGATGGAGAA GTTCCTGCAG AACAAGGAGC TCTTCAGCAG TCTGAAGAAG
    GGGAAGATTT GCTGCTGCTG CCGAGCCAAG TTCCCGCTGT TGTCATGGCC ACCCTCCTGT
    CTCTTTTGCA AGAGAGCCGT CTGCAACTCC TGTAGCATAA AGATGAAGAT GCCTTCTAAG
    AAGTTTGCTC ACATCCCTGT CTACACACTG GGCTTTGAGA GTCCTCAGAG GATGTCAACT
    GCCAAAACCA CACCAACACA GAGAAGAGAC GCCTTCCAAT CCCTGCAAGG GCTGCAGTGG
    CGGAACGTGG AGGAGGAGTT CCCGCACATG TACACCCACG GCTGTGTCAT GAAGGACGTG
    TGCAGCGACT GCACCGGGTT CGTGGCGGAT GTGGTGCACT CCAGCCGCAG GAGCGTGGAG
    GCCCTCAACA CCACGCCACG TCGTGCCCGC CAGACCCAGT CCCTGTATGT CCCGAACACC
    TGGACACTTG ACCTCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.